CN109007848A - It is a kind of to improve sexual function and the preparation method of antifatigue compound oligopeptide, its oral preparation and the oral preparation - Google Patents
It is a kind of to improve sexual function and the preparation method of antifatigue compound oligopeptide, its oral preparation and the oral preparation Download PDFInfo
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- CN109007848A CN109007848A CN201810775281.6A CN201810775281A CN109007848A CN 109007848 A CN109007848 A CN 109007848A CN 201810775281 A CN201810775281 A CN 201810775281A CN 109007848 A CN109007848 A CN 109007848A
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- oligopeptide
- walnut
- oyster
- compound
- sexual function
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
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- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
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Abstract
The present invention is for Chinese herbal medicine existing defect in terms of improving sexual function in the prior art, the preparation method of a kind of raising sexual function and antifatigue compound oligopeptide, its oral preparation and the oral preparation is provided, the compound oligopeptide is aided with Chinese yam polysaccharide and is prepared using oyster oligopeptide and walnut oligopeptide as primary raw material.The oyster oligopeptide and walnut oligopeptides that the compound oligopeptide is obtained using pure natural edible biological material extraction are aided with Chinese yam polysaccharide as primary raw material, for different application condition, prepare the oral preparation of different dosage forms, can effectively relieve fatigue, and improve sexual function.
Description
Technical field
The invention belongs to male sexual function health care product technical fields, and in particular to a kind of to improve the compound oligomeric of sexual function
The preparation method of peptide, its oral preparation and the oral preparation.
Background technique
Sex dysfunction is a kind of common multiple diseases, directly affects the mental health of male, damages goodwill as between spouses,
Jeopardize social stability, additionally with the shape of diabetes, liver and kidney disease, cardiovascular disease, prostatic disorders and mental disease etc.
At there is close ties.Sex dysfunction often makes male generate sense of defeat or self-negation psychology, damages goodwill as between spouses and family
Stablize, and then influences social function.Therefore, how being effectively improved male sexual disfunction, to have become today's society urgently to be resolved
Medical problem, and since clinical medicine has a different degrees of side effect, find safely and effectively native chemical object come improvement property function
Energy obstacle is extremely urgent.
Oyster (Ostrea gigas thunberg), Ostreidae oyster category are commonly called as oyster, and oyster is a kind of rich in albumen
Bivalve marine mollusc, eat and medical value it is very high.Compendium of Material Medica is recorded: " toast maximum phase of an eclipse beauty, makes us thin to oyster meat
Skin, U.S. color, tonifying kidney and strengthening yang, and void can be controlled, and solve erysipelas ", prompt oyster to have certain effect to sexual function is improved.Utilize the modern times
It is sub- that the oyster peptide that biotechnology is extracted from Oyster Protein contains the essential arginine of a large amount of manufacture sperms, microelement
Lead, selenium and vitamin abundant, the former arginine are the main components for manufacturing sperm, and sub- lead promotes hormonal secretion, and
Selenium is also to manufacture the indispensable microelement of sperm.It recent studies have shown that, small molecule oyster polypeptide can pass through raising property function
Can the expression quantity of related gene enhance the sexual function of male mice.
Walnut (Juglans regia L.), alias English walnut, small walnut, Juglans mandshurica, juglans mandshurica etc. are Juglandaceae juglans
Plant.As advanced green food, since ancient times, the healthcare function of walnut is recognized high praise, there is Longevity, long live
Title.Traditional Chinese Medicine thinks walnut natural disposition mild, sweet flavor and nontoxic, there is promoting digestion and enriching blood in the course of disease treatment
The special effect reposed with moistening lung.The study found that long-term consumption hickory nut can apparent pre- anti-aging, prostate cancer, prevent and treat painstaking effort
Pipe disease and improvement sexual function, in addition, applicant is research shows that walnut oligopeptide has antifatigue, anti-oxidant, anti-radiation, raising
The physiological functions such as immune, anti-aging, prompt walnut to have some improvement sexual function.
At present about the publication for improving sexual function is improved, Chinese herbal medicine formula is mostly used, drug or battalion are prepared into
Health care product is supported, Chinese patent (CN101181063A) discloses a kind of nutrition and health care for treating male sterility and sexual disorder
Product, the pharmaceutical composition are mainly made of the following bulk drugs as unit of parts by weight: 20 parts of fructus lycii, 18 parts of silkworm chrysalis, Chinese yam 15
Part, 15 parts of cortex cinnamomi, 13 parts of mulberry fruit, 12 parts of fructus alpiniae oxyphyllae, 12 parts of dateplum persimmon and 10 parts of pollen pini.Chinese patent (CN101766778A) is public
Cloth a kind of raising male's sexual and the Chinese medicine composition of the treatment of sexual dysfunction and preparation method thereof, mainly by dietotherapeutic
Chinese medicine root of kirilow rhodiola, RADIX POLYGONI MULTIFLORI PREPARATA, Cortex Eucommiae, fructus alpiniae oxyphyllae, Semen Cuscutae, Radix Astragali, Chinese yam, fructus lycii, semen astragali complanati, turtle shell, Semen sesami nigrum, Fu
Siberian cocklebur, dens draconis, Radix Polygalae, radix cyathulae, jujube etc. are process.Although its formula of the patent announced contains Chinese yam, main component
It is still Chinese herbal medicine, needs to be considered in safety, and takes for a long time and be possible to also indefinite to human body bring side effect.
Summary of the invention
The present invention provides a kind of raising property function for Chinese herbal medicine existing defect in terms of improving sexual function in the prior art
Can and antifatigue compound oligopeptide and its oral preparation, the compound oligopeptide obtained with pure natural edible biological material extraction
Oyster oligopeptide and walnut oligopeptide be primary raw material, be aided with Chinese yam polysaccharide, for different application condition, prepare different dosage forms
Oral preparation, can effectively relieve fatigue, improve sexual function.
The present invention adopts the following technical scheme:
A kind of to improve sexual function and antifatigue compound oligopeptide, the compound oligopeptide is low with oyster oligopeptide and walnut
Poly- peptide is primary raw material, is aided with Chinese yam polysaccharide and is prepared.
Further, in terms of the compound oligopeptide of 1kg, dosage of each component is as follows: oyster oligopeptide 150-650g, walnut are oligomeric
Peptide 100-550g, surplus are Chinese yam polysaccharide.
Further, the oyster oligopeptide is the small molecule bioactive extracted from oyster using biological enzymolysis technology
Peptide, oligopeptide of the relative molecular mass less than 1000 account for 90% or more.
Further, enzyme used in the biological enzymolysis technology be alkali protease (2.4AU/g, Novi of Denmark letter),
Neutral proteinase (0.8AU/g, Novi of Denmark letter), papain (2000U/g, Wolsen company), trypsase
One of (1250USP/mg, Novi of Denmark letter) and flavor protease (1000LAPU/g, Novi of Denmark letter).
Further, the oyster oligopeptide will also carry out flavoring processing after extracting, and the flavoring processing is to pass through high score
Sub- substance embeds the oyster oligopeptide after separation, and polymer substance used is beta-cyclodextrin, maltodextrin, soybean separation
One of albumen and N-LOK converted starch, wherein N-LOK converted starch is purchased from National starch&chemical
Ltd. the U.S..
The specific extraction process of the oyster oligopeptide are as follows: fresh oyster --- go out by decladding mashing --- enzymolysis processing ---
--- --- --- concentration --- tender taste processing --- is dried separating treatment clarifying treatment enzymatic treatment.
Wherein, the described decladding mashing is removal oyster shell, takes oyster meat, when mashing, the mass ratio of water and oyster meat are as follows:
1-5:1 obtains oyster slurries；
The parameter of the enzymolysis processing are as follows: hydrolysis temperature is 40-65 DEG C, pH 4-10, time 4-10h, enzyme addition
Amount is the 0.5-5% of oyster grind slurries quality, obtains enzymolysis liquid after enzymatic hydrolysis；Wherein, enzyme can select alkali protease (2.4AU/g, pellet
Wheat Novi letter), neutral proteinase (0.8AU/g, Novi of Denmark letter), papain (2000U/g, Wolsen company), pancreas egg
One or more of white enzyme (1250USP/mg, Novi of Denmark letter) and flavor protease (1000LAPU/g, Novi of Denmark letter).
The destroy the enzyme treatment are as follows: enzymolysis liquid is heated to boil, and keep 5-15min by boiling water enzyme deactivation 5-15min.
The clarifying treatment are as follows: take supernatant spare using centrifuge separation enzymolysis solution after enzyme deactivation, centrifuge separation uses
10000-15000r/min is centrifuged 10-20min.
The separating treatment uses UF membrane or chromatography, and UF membrane carries out ultrafiltration using cellulosic ultrafiltration membrane, will
It is 10ku, 5ku, 3ku, 1ku ultrafiltration membrane below, feed pressure that supernatant obtained by clarifying treatment, which passes sequentially through molecular cut off,
0.6Mpa-1.0Mpa, temperature are 20 DEG C -30 DEG C；Chromatography is separated using gel chromatography, successively uses Sephadex G-25
It is separated with Sephadex G-10, mobile phase is deionized water.
The concentration is that nanofiltration or vacuum concentration are handled, and obtains concentrate, wherein the technological parameter of nanofiltration are as follows: pressure
0.1-1MPa, 25-50 DEG C of temperature, time 10-100min, the technological parameter of vacuum concentration are as follows: 30-60 DEG C, pressure 0.5-
1MPa。
The flavoring processing is that the oyster oligopeptide after verifying separation by polymer is embedded, selected high score
Son is one of beta-cyclodextrin, maltodextrin, soybean protein isolate and N-LOK converted starch, the additive amount of polymer substance
For the 0.1-10% of concentrate quality, embedding uses high-pressure homogeneous or high pressure microjet method, wherein high-pressure homogeneous technique
Parameter are as follows: pressure 20-120MPa, number 1-3 times, 30-60 DEG C of temperature, the technological parameter of high pressure microjet are as follows: pressure 20-
150MPa, number 1-3 times.
The drying process is using spray drying or vacuum freeze drying, the condition of spray drying are as follows: spray drying import
160-200 DEG C of temperature, 80-85 DEG C of outlet temperature；Vacuum freeze drying condition are as follows: vacuum degree 0.014MPa, condenser temperature be-
40 DEG C -- 60 DEG C, drying time 20-30h, up to powdered oyster oligopeptide after drying.
Further, the walnut oligopeptide is the small molecule biology extracted from walnut using biological enzymolysis technology
Active peptide, oligopeptide of the relative molecular mass less than 1000 account for 80% or more.
Further, the biological enzymolysis technology use stepwise discretization method, and successively using alkali protease (2.4AU/g,
Novozymes Company of Denmark), neutral proteinase (0.8AU/g, Novozymes Company of Denmark) and flavor protease (1000LAPU/g, it is red
Wheat Novozymes Company) carry out enzymolysis processing.
Further, before the walnut oligopeptide extracts, decortication processing and ungrease treatment are carried out to walnut kernel in advance.
The specific extraction process of the walnut oligopeptide are as follows: walnut kernel peeling handles --- ungrease treatment --- at homogenate
--- --- --- clarifying treatment --- separating treatment --- is dried destroy the enzyme treatment enzymolysis processing reason.
Wherein, the walnut kernel peeling processing uses hot-water soak or enzymatic isolation method, the technological parameter of hot-water soak are as follows: 70-
90 DEG C, 30-60min；The solid-liquid ratio of enzymatic isolation method selection cellulase, walnut kernel and water is 1:3 (mass/volume), enzyme additive amount
For the 0.1-0.5% of walnut kernel quality, enzymolysis time 20min-60min, 35-60 DEG C of temperature.
The ungrease treatment uses subcritical fluid extraction technology, using the ethanol water that mass fraction is 60% as entrainment
Agent, 100-160 DEG C of temperature, pressure 5-10MPa, time 20-60min takes walnut kernel spare after extraction.
The homogenized uses high-pressure homogeneous, parameter are as follows: pressure 20-120MPa, number 1-3 times, and 30-60 DEG C of temperature,
Walnut slurries are obtained after homogenized.
The enzymolysis processing use stepwise discretization method, and using alkali protease (2.4AU/g, Novozymes Company of Denmark),
Neutral proteinase (0.8AU/g, Novozymes Company of Denmark) and flavor protease (1000LAPU/g, Novozymes Company of Denmark), enzyme
Order of addition are as follows: first add alkali protease (additive amount be walnut grind slurries quality 0.2-1%, pH8-10,40-60 DEG C of temperature, when
Between 0.5-2h), then add neutral proteinase (additive amount be walnut grind slurries quality 0.2-1%, pH6-8,40-60 DEG C of temperature, the time
1-3h), finally add flavor protease (additive amount be walnut grind slurries quality 0.2-1%, pH6-8,30-50 DEG C of temperature, the time
0.5-1h), enzymolysis liquid is obtained after enzymatic hydrolysis.
Destroy the enzyme treatment is high temperature enzyme deactivation method, enzymolysis liquid is placed in the water-bath that temperature is 90-100 DEG C, and 10-30min is handled.
After the completion of enzyme deactivation, the clarifying treatment takes supernatant spare using centrifuge separation, and centrifuge separation uses 4000-
10000r/min is centrifuged 10-30min.
The separating treatment uses UF membrane or chromatography, and UF membrane carries out ultrafiltration using cellulosic ultrafiltration membrane, will be clear
It is 10ku, 5ku, 3ku, 1ku ultrafiltration membrane below, feed pressure that clear processing gained supernatant, which passes sequentially through molecular cut off,
0.6Mpa-1.0Mpa, temperature are 20 DEG C -30 DEG C；Chromatography using gel chromatography separate, successively using Sephadex G-25,
Sephadex G-10 is separated, and mobile phase is deionized water.
The drying process is using spray drying or vacuum freeze drying, the condition of spray drying are as follows: spray drying import
160-200 DEG C of temperature, 80-85 DEG C of outlet temperature；Vacuum freeze drying condition are as follows: vacuum degree 0.014MPa, condenser temperature be-
40 DEG C -- 60 DEG C, drying time 20-30h；Up to powdered walnut oligopeptide after drying.
The oral preparation of above-mentioned raising sexual function and antifatigue compound oligopeptide, the dosage form of the oral preparation include
Effervescent tablet, solid beverage and oral solution；Compound oligopeptide of the present invention is extracted with pure natural raw material and is obtained, and does not add chemical conjunction
At preservative, bacteriostatic agent, corrigent, not only can be used as food consumption, be also used as nourishing ingredient and make an addition to all kinds of foods
In product, at the same oral preparation of its preparation eat it is easy to carry, be it is a kind of it is highly-safe, absorb rapidly, fatigue is effectively relieved, mentions
The novel foodstuff of high sexual function.
The preparation method of above-mentioned raising sexual function and antifatigue compound oligopeptide effervescent tablet, comprising the following steps:
(1) premix is handled: oyster oligopeptide, walnut oligopeptide and Chinese yam polysaccharide are mixed by above-mentioned consumption proportion,
And disintegrating agent is added and stirs and evenly mixs, obtain premix；
Wherein, oyster oligopeptide, walnut oligopeptide and Chinese yam polysaccharide are mixed by following consumption proportion: compound with 1kg
Oligopeptide meter: oyster oligopeptide 150-650g, walnut oligopeptides 100-550g, surplus is Chinese yam polysaccharide；
The disintegrating agent is that citric acid, tartaric acid and malonic acid are one such, and disintegrating agent additive amount is compound oligomeric
Disintegrating agent is dissolved in after forming solution in 70 DEG C of distilled water and is added in compound oligopeptide by the 5-50% of peptide quality；
(2) pelletization treatment: the premix that step (1) obtains is pelletized, and obtains solid particle, and granulation method therefor is
High-shearing granulation combination fluidized bed drying or spray-drying process.
The high-shearing granulation condition is that agitating paddle shear rate is 100-500r/min, cutter rotating velocity 200-
Whole grain, drying condition is dried by fluidized bed after the completion of wet granular is granulated in 2000r/min, Granulation time 1-15min are as follows:
Inlet air temperature is 50-80 DEG C, intake 1000-3000m3/ h, control particle water content discharge in 1%-5%, by dry
Grain crosses 1.5mm sieve whole grain.
The spray drying granulation is spray-dried 180-220 DEG C of inlet temperature, outlet using Spray Grain-make Drier
90-120 DEG C of temperature.
(3) compressing tablet process: the solid particle and mix lubricant that step (2) is obtained carry out compression molding, obtain effervesce
Piece；
The lubricant is one of magnesium stearate or polyethylene glycol, and additive amount is the 1-5% of mass of solid particles, pressure
Piece pressure is 0.2-1.5Mpa.
(4) sterilization packaging: the effervescent tablet that step (3) is obtained carries out radiation sterilization, packaging.
The preparation method of above-mentioned raising sexual function and antifatigue compound oligopeptide solid beverage, comprising the following steps:
(1) premix is handled: oyster oligopeptide, walnut oligopeptide and Chinese yam polysaccharide are mixed by above-mentioned consumption proportion,
It is pulverized, smashed compound oligopeptide crosses 100-500 mesh, obtains compound oligopeptide powder；
Wherein, oyster oligopeptide, walnut oligopeptide and Chinese yam polysaccharide are mixed by following consumption proportion: compound with 1kg
Oligopeptide meter: oyster oligopeptide 150-650g, walnut oligopeptide 100-550g, surplus is Chinese yam polysaccharide；
(2) preparation of compound oligopeptide solution: compound oligopeptide powder is dissolved in 60-80 DEG C of hot water, is sufficiently mixed
Even, progress is high-pressure homogeneous, obtains compound oligopeptide solution；Wherein, high-pressure homogeneous condition is pressure 20-100MPa, number 1-3
It is secondary；
(3) concentration: the resulting compound oligopeptide solution of step 2) being used and is concentrated in vacuo, vacuum degree 0.5-1MPa,
60-80 DEG C of temperature, obtain concentrate；
(4) dry: the resulting concentrate in rapid 3) being spray-dried, is spray-dried 120-160 DEG C of inlet temperature, outlet
65-85 DEG C of temperature；
(5) sterilization packaging: radiation sterilization, irradiation dose 5kGy-35kGy, packaging.
The preparation method of above-mentioned raising sexual function and antifatigue compound oligomeric peptide oral liquid, includes the following steps:
(1) preparation of compound oligopeptide solution: oyster oligopeptide, walnut oligopeptide and Chinese yam polysaccharide are matched by above-mentioned dosage
Than being mixed and being dissolved in distilled water, stir；
(2) preparation of seasoning liquid: sweetener and acidity regulator being dissolved in compound oligopeptide solution, are sufficiently stirred,
Using filter-cloth filtering, seasoning liquid is obtained；
Wherein, the sweetener is that honey or xylitol are one of, and additive amount is the 1-10% of solution quality, acid
Degree regulator is that citric acid, lactic acid or malic acid are one of, and additive amount is the 0.2-2% of solution quality.The filter cloth mistake
Filter, the filter cloth mesh number 100-200 mesh of use；
(3) the resulting seasoning liquid of step 2) homogenization: is subjected to high-pressure homogeneous processing；
Wherein, the high-pressure homogeneous condition is pressure 10-50MPa, 40-65 DEG C of temperature, number 1-2 times；
(4) sterilization treatment: using high-temperature short-time sterilization for liquid obtained by rapid 3), and actual temp is 100-130 DEG C, and the time is
10-60s；
(5) filling process: by step (4) sterilized liquid it is filling in the container of disinfection to get finished product；Wherein, filling temperature
Degree is 80-95 DEG C, and the disinfection is sterilized using chlorine water.
Beneficial effects of the present invention are as follows:
Walnut oligopeptide prepared by the present invention is by walnut kernel by decortication and ungrease treatment, and then passes through biological enzymolysis skill
Art is prepared.
Since walnut kernel brown endotesta contains phenols and tannin, the mouthfeel and color of walnut product often will affect
Pool, also, this substance can cause protein solubility to decline, to reduce walnut oligopeptide with protein covalent bond
Yield, so needing to remove it.The method for generalling use dipping by lye at present carries out the decortication processing of walnut kernel, this method has
Following drawback: 1) by alkaline process immersion treatment, the content of walnut kernel essential amino acid can decline, and the type of limiting amino acid increases
Add, amino acid score decline；2) spent lye has some impact on environment, and the institute's effluent of walnut kernel lye dipping workshop is Huang
Black troubled liquor, surface are and organic containing lipid, protein and walnut kernel powder etc. with white foam and a large amount of suspended matters
Object, each contamination index of the waste water is higher than national secondary discharge standard after measured, and having to pass through wastewater treatment can discharge, this
Sample considerably increases production cost.And the hot-water soak processing used in the present invention and enzymolysis processing, walnut kernel nutritional ingredient damage
It loses less and pollution-free, is a kind of green, efficient method.
To reduce fat content in product, protein active in walnut kernel is protected, the yield of protein is improved, usually by walnut
Benevolence carries out ungrease treatment.Currently used degreasing method can cause walnut egg frequently with organic solvent method degreasing, hot environment
The denaturation of white matter and the loss of other nutritional ingredients, and organic solvent also results in certain pollution to environment.What the present invention used
Subcritical fluid techniques degreasing, actually to the abstraction technique of grease, due to subcritical fluid extraction grease, extraction cycle consumption
When it is short, and extraction yield is high, and walnut oil colours is shallow, bright, not will cause the denaturation of walnut kernel albumen, and one side can reach in this way
To the purpose of degreasing, on the other hand, the walnut oil extracted is best in quality, can be developed further into Related product.
Therefore, by peeling above, defatting technology, remain the nutriment of walnut kernel to the maximum extent, and improve
The yield of walnut oligopeptide.
The intrinsic fishy smell of oyster, and due to producing small peptide and micro hardship in the enzymatic hydrolysis preparation process of oyster oligopeptide
Flavor nucleotide has certain adverse effect to sense organ.The present invention has carried out flavoring processing to the oyster oligopeptide of enzymatic hydrolysis preparation.Mesh
Before to be usually used in the technology of flavoring and taste masking include: addition corrigent, bitter tasting retarding agent, taste bud paralyzant, coating, amberlite
Rouge technology etc..Inclusion technique is the flavoring technology for continuing to develop and occurring recently as Modern preparations technology.The present invention is for the first time
Flavoring processing is carried out to oyster oligopeptide using inclusion technique, compared to using additive, highly-safe, significant effect, simultaneously
Easy to operate compared to ion-exchange-resin process, production cost is low.The present invention uses high pressure homogenization technique and high pressure microjet skill
Art is included, and is very significantly improved by the high-pressure homogeneous partial size for handling obtained emulsion and dispersibility.With it is traditional
High-pressure homogeneous mode is compared, and high pressure microjet processing pressure is higher, and faster, collision energy is bigger for fluid velocity, and product particle is more
Carefully, up to nanoscale, to improve stability of solution, the oyster oligopeptide embedding rate by flavoring processing is high with this condition,
Delicate mouthfeel.
In the preparation process of biologically active peptide, in order to obtain the peptide fragment of high activity, enzymolysis process is vital.Enzymatic hydrolysis
Technique is broadly divided into single enzyme and multi-enzymatic hydrolysis method, and multi-enzymatic hydrolysis method is divided into mixed enzyme and substep enzyme hydrolysis method.Production at present
Using it is most be single enzymolysis method, this method simple process, but generally oligomeric compared with high yield pulp1 and relatively small molecular weight in order to obtain
Peptide, then it is more to consume enzyme amount, takes a long time, product has stronger bitter taste and more salinity.And the complex enzyme applied in the present invention point
Step enzymatic isolation method prepares oligopeptide, and less enzyme amount and short period can get the lesser oligopeptide of molecular weight, and better assure that
The functional activity of polypeptide.In the present invention, complex enzyme zymohydrolysis condition can be controlled by substep to control degree of hydrolysis, to obtain
The polypeptide of required molecular weight ranges.Oyster oligopeptide and walnut oligopeptide prepared by the present invention are being visited using degree of hydrolysis as index
It has begged in the theory of enzymolysis kinetics, has optimized enzyme digestion reaction parameter, the digestion action site based on different enzymes passes through hydrolysis
Degree, oligomeric peptide yield and amino acid form to screen the type of enzyme and optimization enzymolysis process, are less than so that molecular weight be prepared
1000 oligopeptide, the advantages of reaching absorptivity high, good biocompatibility.
Specific embodiment
In order to keep technical purpose of the invention, technical scheme and beneficial effects clearer, combined with specific embodiments below
Technical solution of the present invention is further illustrated.
The preparation embodiment 1 of oyster oligopeptide:
(1) decladding is beaten: taking fresh oyster, decladding takes oyster meat, adds water, and the mass ratio of water and oyster meat is 1:1, breaks into
Slurries；
(2) enzymolysis processing: enzymolysis processing is carried out to slurries obtained by step (1) using biological enzymolysis technology, enzyme used is selected
Alkali protease (2.4AU/g, Novi of Denmark letter), enzyme additive amount is the 2% of oyster grind slurries quality, and hydrolysis temperature is 50 DEG C, pH
It is 8, enzymolysis time 6h obtains enzymolysis liquid, degree of hydrolysis 20.3%；
(3) enzymolysis liquid obtained by step (2) destroy the enzyme treatment: is subjected to boiling water enzyme deactivation 10min；
(4) clarifying treatment: the enzymolysis liquid after step (3) destroy the enzyme treatment takes supernatant spare using centrifuge separation, centrifugation point
From 10000r/min is used, it is centrifuged 10min；
(5) separating treatment: supernatant obtained by step (4) is used into UF membrane, UF membrane selects cellulosic ultrafiltration membrane to carry out
Ultrafiltration, it is 10ku, 5ku, -3ku, 1ku ultrafiltration membrane below, feed pressure when ultrafiltration that supernatant, which passes sequentially through molecular cut off,
1.0Mpa, temperature are 30 DEG C, obtain ultrafiltrate；
(6) method for concentration: ultrafiltrate is concentrated using nanofiltration, the technological parameter of nanofiltration are as follows: pressure 1MPa, temperature 30
DEG C, time 100min obtains concentrate；
(7) flavoring is handled: carrying out embedding treatment to concentrate obtained by step (6), embedding treatment selects beta-cyclodextrin, addition
Amount is the 1% of concentrate quality, passes through high-pressure homogeneous method and completes embedding, specific technological parameter are as follows: pressure 100MPa, it is secondary
Number 3 times, 30 DEG C of temperature；
(8) be dried: to mixture obtained by step (7) embedding treatment using spray drying, condition are as follows: be spray-dried into
200 DEG C of temperature, 85 DEG C of outlet temperature of mouth；It is dry to complete up to oyster oligopeptide powder.
By trichloroacetic acid soluble nitrogen detection method, the small peptide content of oyster oligopeptide powder is 88%.
It is detected through HPLC MS, in the extracted oyster oligopeptide of embodiment 1, relative molecular mass is small
It in 1000 oligopeptide accounting 98.21%, is found through aas determination, content of beary metal is low, in the remaining embodiments
Determining heavy metals are suitable with the testing result of embodiment 1, therefore long-term use will not influence human health.
The molecular weight distribution of 1 oyster oligopeptide of table
2 oyster oligopeptide heavy metal analysis result of table
The preparation embodiment 2 of oyster oligopeptide:
(1) decladding is beaten: taking fresh oyster, decladding takes oyster meat, adds water, the mass ratio of water and oyster meat are as follows: 2:1,
Break into slurries；
(2) enzymolysis processing: enzymolysis processing is carried out to slurries obtained by step (1) using biological enzymolysis technology, enzyme used is selected
Neutral proteinase (0.8AU/g, Novi of Denmark letter), enzyme additive amount is the 2% of substrate quality, and hydrolysis temperature is 40 DEG C, pH 7,
Time is 8h, obtains enzymolysis liquid, degree of hydrolysis 19.4%；
(3) enzymolysis liquid obtained by step (2) destroy the enzyme treatment: is subjected to boiling water enzyme deactivation 10min；
(4) clarifying treatment: the enzymolysis liquid after step (3) destroy the enzyme treatment takes supernatant spare using centrifuge separation, centrifugation point
From 15000r/min is used, it is centrifuged 10min；
(5) separating treatment: supernatant obtained by step (4) is used into UF membrane, UF membrane selects cellulosic ultrafiltration membrane to carry out
Ultrafiltration, it is 10ku, 5ku, 3ku, 1ku ultrafiltration membrane below, feed pressure when ultrafiltration that supernatant, which passes sequentially through molecular cut off,
0.8Mpa, temperature are 20 DEG C, obtain ultrafiltrate；
(6) method for concentration: ultrafiltrate is using vacuum concentration, the technological parameter of vacuum concentration are as follows: temperature 50 C, pressure are
0.8MPa obtains concentrate；
(7) flavoring is handled: embedding treatment is carried out to concentrate obtained by step (6), embedding treatment selects soybean protein isolate,
Additive amount is the 5% of concentrate quality, passes through high pressure microjet and completes embedding, specific technological parameter are as follows: pressure 50MPa, it is secondary
Number 1 time, 20 DEG C of temperature；
(8) it is dried: to mixture obtained by step (7) embedding treatment using spray drying.Condition are as follows: be spray-dried into
180 DEG C of temperature, 80 DEG C of outlet temperature of mouth；It is dry to complete up to oyster oligopeptide powder.
By trichloroacetic acid soluble nitrogen detection method, the small peptide content of oyster oligopeptide powder is 78.1%.
It is detected through HPLC MS, in the extracted oyster oligopeptide of embodiment 2, relative molecular mass is small
In 1000 oligopeptide accounting 96.27%.
The molecular weight distribution of 3 oyster oligopeptide of table
The preparation embodiment 3 of oyster oligopeptide:
(1) decladding is beaten: taking fresh oyster, decladding takes oyster meat, adds water, the mass ratio of water and oyster meat are as follows: 5:1,
Break into slurries；
(2) enzymolysis processing: enzymolysis processing is carried out to slurries obtained by step (1) using biological enzymolysis technology, enzyme used is selected
Trypsase (1250USP/mg, Novi of Denmark letter), enzyme additive amount is the 3% of substrate quality, and hydrolysis temperature is 50 DEG C, pH 7,
Time is 10h, obtains enzymolysis liquid, degree of hydrolysis 40.0%；
(3) enzymolysis liquid obtained by step (2) destroy the enzyme treatment: is subjected to boiling water enzyme deactivation 10min；
(4) clarifying treatment: the enzymolysis liquid after step (3) destroy the enzyme treatment takes supernatant spare using centrifuge separation, centrifugation point
From 15000r/min is used, it is centrifuged 20min；
(5) separating treatment: supernatant obtained by step (4) is separated using gel chromatography, successively uses Sephadex G-
25, Sephadex G-10 is separated, and mobile phase is deionized water, obtains separating liquid；
(6) concentration, the technological parameter of nanofiltration method for concentration: are carried out using nanofiltration to separating liquid obtained by step (6) are as follows:
Pressure 1MPa, 25 DEG C of temperature, time 100min obtains concentrate；
(7) flavoring is handled: carrying out embedding treatment to concentrate obtained by step (6), embedding treatment selects N-LOK denaturation to form sediment
Powder, additive amount are the 10% of concentrate quality, complete embedding, specific technological parameter by the method for high pressure microjet are as follows: pressure
Strong 150MPa, number 3 times, 20 DEG C of temperature；
(8) it is dried: vacuum freeze drying, condition are as follows: vacuum degree is used to mixture obtained by step (7) embedding treatment
For 0.014MPa, condenser temperature is -60 DEG C, drying time 30h；It is dry to complete up to oyster oligopeptide powder.
By trichloroacetic acid soluble nitrogen detection method, the small peptide content of oyster oligopeptide powder is 90.1%.
It is detected through HPLC MS, in the extracted oyster oligopeptide of embodiment 3, relative molecular mass is small
In 1000 oligopeptide accounting 99.07%.
The molecular weight distribution of 4 oyster oligopeptide of table
The preparation embodiment 1 of walnut oligopeptide:
(1) decortication is handled: being taken walnut kernel to carry out decortication processing using hot-water soak, 80 DEG C of hot water temperature, is impregnated 30min；
(2) ungrease treatment: the walnut meat after peeling uses subcritical-fluid extraction technology, is 60% with mass fraction
Ethanol water is entrainer, and 120 DEG C of temperature, pressure 5Mpa, time 30min take walnut kernel spare after extraction；
(3) homogenized: the pretreated walnut kernel of step (2) is subjected to homogenized, homogenized is using high-pressure homogeneous
Method, pressure 50MPa, number 2 times, 40 DEG C of temperature；
(4) slurries after step (3) homogenized enzymolysis processing: are used into alkali protease (2.4AU/g, Novi of Denmark
Letter company), neutral proteinase (0.8AU/g, Novozymes Company of Denmark) and flavor protease (1000LAPU/g, Novi of Denmark letter
Company) carry out enzymolysis processing, the order of addition of enzyme are as follows: and first adding alkali protease, (additive amount is walnut grind slurries quality
0.2%, pH10,40 DEG C of temperature, time 2h), then add neutral proteinase (additive amount is 0.5%, pH7 of walnut grind slurries quality,
Temperature 50 C, time 2h), finally add flavor protease (additive amount be walnut grind slurries quality 1%, pH7, temperature 50 C, when
Between 0.5h), obtain enzymolysis liquid, degree of hydrolysis 35.2%；
(5) destroy the enzyme treatment: enzymolysis liquid obtained by step (4) is subjected to water-bath destroy the enzyme treatment, temperature is 95 DEG C, water-bath 20min；
(6) clarifying treatment: step (5) enzymolysis solution after enzyme deactivation is centrifuged using 4000r/min, centrifugation
20min takes supernatant；
(7) separating treatment: ultrafiltration is carried out using cellulosic ultrafiltration membrane to supernatant obtained by step (6), supernatant successively leads to
Crossing molecular cut off is 10ku, 5ku, 3ku, and 1ku ultrafiltration membrane below, feed pressure 0.8Mpa when ultrafiltration, temperature is 30 DEG C,
Obtain ultrafiltrate；
(8) it is dried: to ultrafiltrate obtained by step (7) using spray drying, being spray-dried 200 DEG C of inlet temperature, out
85 DEG C of temperature of mouth obtains walnut oligopeptide powder after the completion of dry.
By trichloroacetic acid soluble nitrogen detection method, the small peptide content of walnut oligopeptide powder is 78.1%.
It is detected through HPLC MS, in walnut oligopeptide, oligopeptide of the relative molecular mass less than 1000 is accounted for
It than 86.5%, detects and finds through atomic absorption light spectral method, content of beary metal is low, in the remaining embodiments determining heavy metals and implementation
The testing result of example 1 is suitable, and long-term use will not influence human health；.
The molecular weight distribution of 5 walnut oligopeptide of table
6 walnut oligopeptide heavy metal analysis result of table
The preparation embodiment 2 of walnut oligopeptide:
(1) decortication is handled: being taken walnut kernel to use and is carried out decortication processing using enzymatic isolation method, selects cellulase herein, enzyme adds
Dosage be walnut quality 0.1%, enzymolysis time 20min, 40 DEG C of temperature；
(2) ungrease treatment: the walnut meat after peeling uses subcritical-fluid extraction technology, with mass fraction for 60% second
Alcohol solution is entrainer, and 120 DEG C of temperature, pressure 5Mpa, time 40min take walnut kernel spare after extraction；
(3) homogenized: the pretreated walnut kernel of step (2) is subjected to homogenized, homogenized is using high-pressure homogeneous
Method, pressure 80MPa, number 3 times, temperature 60 C；
(4) slurries after step (3) homogenized enzymolysis processing: are used into alkali protease (2.4AU/g, Novi of Denmark
Letter company), neutral proteinase (0.8AU/g, Novozymes Company of Denmark) and flavor protease (1000LAPU/g, Novi of Denmark letter
Company) carry out enzymolysis processing, enzyme order of addition are as follows: first add alkali protease (additive amount be walnut grind slurries quality 1%,
PH8,40 DEG C of temperature, time 2h), then add neutral proteinase (additive amount is 1%, pH8 of walnut grind slurries quality, temperature 60 C,
Time 3h), finally add flavor protease (additive amount be walnut grind slurries quality 0.5%, pH8,40 DEG C of temperature, time 1h),
Obtain enzymolysis liquid, degree of hydrolysis 38.9%；
(5) destroy the enzyme treatment: enzymolysis liquid obtained by step (4) is subjected to water-bath destroy the enzyme treatment, temperature is 95 DEG C, water-bath 20min；
(6) clarifying treatment: step (5) enzymolysis solution after enzyme deactivation is centrifuged using 10000r/min, centrifugation
10min takes supernatant；
(7) separating treatment: ultrafiltration is carried out using cellulosic ultrafiltration membrane to supernatant obtained by step (6), supernatant successively leads to
Crossing molecular cut off is 10ku, 5ku, 3ku, 1ku ultrafiltration membrane below；Feed pressure 1.0Mpa, temperature are 30 DEG C, obtain ultrafiltration
Liquid；
(8) it is dried: vacuum freeze drying, condition is used to ultrafiltrate obtained by step (7) are as follows: vacuum degree is
0.014MPa, condenser temperature are -50 DEG C, drying time 30h；Walnut oligopeptide powder is obtained after the completion of dry.
By trichloroacetic acid soluble nitrogen detection method, the small peptide content of walnut oligopeptide powder is 70.8%.
It is detected through HPLC MS, in walnut oligopeptide, oligopeptide of the relative molecular mass less than 1000 is accounted for
Than 87.5%.
The molecular weight distribution of 7 walnut oligopeptide of table
The preparation embodiment 3 of walnut oligopeptide:
(1) decortication is handled: taking walnut kernel to carry out decortication processing using enzymatic isolation method, enzymatic isolation method selects cellulase, enzyme addition
Amount is the 0.5% of walnut quality, enzymolysis time 60min, temperature 60 C；
(2) ungrease treatment: the walnut meat after peeling uses subcritical-fluid extraction technology, with mass fraction for 60% second
Alcohol water is entrainer, and 160 DEG C of temperature, pressure 10Mpa, time 20min take walnut kernel spare after extraction；
(3) homogenized: the pretreated walnut kernel of step (2) is subjected to homogenized, homogenized is using high-pressure homogeneous
Method, pressure 20-120MPa, number 1 time, 30 DEG C of temperature；
(4) slurries after step (3) homogenized enzymolysis processing: are used into alkali protease (2.4AU/g, Novi of Denmark
Letter company), neutral proteinase (0.8AU/g, Novozymes Company of Denmark) and flavor protease (1000LAPU/g, Novi of Denmark letter
Company) carry out enzymolysis processing, enzyme order of addition are as follows: first add alkali protease (additive amount be walnut grind slurries quality 0.2%,
PH10, temperature 60 C, time 0.5h), then add neutral proteinase (1%, pH7 of the additive amount for walnut grind slurries quality, temperature 60
DEG C, time 1h), flavor protease (additive amount is 1%, pH6 of walnut grind slurries quality, temperature 50 C, time 1h) is finally added,
Obtain enzymolysis liquid, degree of hydrolysis 40.1%；
(5) destroy the enzyme treatment: enzymolysis liquid obtained by step (4) is subjected to water-bath destroy the enzyme treatment, temperature is 95 DEG C, water-bath 20min；
(6) clarifying treatment: step (5) enzymolysis solution after enzyme deactivation is centrifuged using 10000r/min, centrifugation
30min takes supernatant；
(7) separating treatment: supernatant obtained by step (6) is separated using gel chromatography, successively uses Sephadex G-
25, Sephadex G-10 is separated, and mobile phase is deionized water, obtains separating liquid；
(8) it is dried: vacuum freeze drying, condition is used to separating liquid obtained by step (7) are as follows: vacuum degree is
0.014MPa, condenser temperature are -60 DEG C, drying time 20h；Walnut oligopeptide powder is obtained after the completion of dry.
By trichloroacetic acid soluble nitrogen detection method, the small peptide content of walnut oligopeptide powder is 81.5%.
It is detected through HPLC MS, in walnut oligopeptide, oligopeptide of the relative molecular mass less than 1000 is accounted for
Than 89.5%.
The molecular weight distribution of 8 walnut oligopeptide of table
Compound oligopeptide effervescent tablet embodiment 1
(1) premix is handled: oyster oligopeptide, walnut oligopeptide and Chinese yam polysaccharide are pressed following dosage: oyster oligopeptide
150g is added 70 DEG C of citric acid solution, stirs and evenly mixs, premixed after walnut oligopeptide 550g, Chinese yam polysaccharide 300g mixing
Material；Wherein, the dosage of citric acid is the 20% of compound oligopeptide quality, and disperses suitable 70 DEG C of distilled water for citric acid and make
With；
(2) whole grain is handled: the premix that step (1) obtains being pelletized using wet granulation, obtains solid particle；System
Grain uses high-shearing granulation, condition are as follows: agitating paddle shear rate is 100r/min, cutter rotating velocity 200r/min, when granulation
Between 5min, wet granular be granulated after the completion of, whole grain, drying condition are dried by fluidized bed are as follows: inlet air temperature be 60 DEG C, air inlet
2000m3/h is measured, control particle water content discharges at 3%, and dry particle is crossed 1.5mm sieve whole grain.
(3) compressing tablet process: the solid particle that step (2) obtains is mixed with magnesium stearate, is carried out compression molding, is obtained effervesce
Piece；Wherein, stearic acid additive amount is the 5% of mass of solid particles, tableting pressure 1Mpa；
(4) packaging sterilizing: by effervescent tablet radiation sterilization, the irradiation dose of irradiation sterilization is 25kGy, packaging.
After tested, the effervescent tablet of the embodiment 1 preparation, in water disintegration time 180s, pH5.8, gas production 80mL, hardness
48N, friability 0.8% are dissolved in 200mL water, and solution is in faint yellow, clear, free from extraneous odour.
It improves sexual function measure of merit: this effervescent tablet solution being subjected to stomach-filling intervention to bull ICR mouse, is intervened
45d assesses its sexual function as intervention group；Blank group (stomach-filling equivalent aqua sterilisa), blank group bull ICR are set simultaneously
Mouse rides incubation period 1.67s, rides number 7 times, mates 2 times, and compound oligopeptide effervescent tablet intervention group, mouse ride latent
Volt phase 0.45s rides number 11 times, mates 3 times；Wherein intervention group and blank group are respectively provided with 6 mouse, and to be randomly assigned,
Similarly hereinafter.
Antifatigue measure of merit: this effervescent tablet solution is subjected to stomach-filling intervention to bull ICR mouse, intervenes 30d, makees
For intervention group, its antifatigue effect is assessed, while blank group (stomach-filling equivalent aqua sterilisa) is set, comparison mice burden swimming power exhausts
Time, blank group bull ICR mouse be 5 ± 0.8min, and the mouse of compound oligopeptide effervescent tablet intervention group be 8.5 ±
0.7min, antifatigue significant effect.
Compound oligopeptide effervescent tablet embodiment 2
(1) premix is handled: oyster oligopeptide, walnut oligopeptide and Chinese yam polysaccharide are pressed following dosage: oyster oligopeptide
400g is added 70 DEG C of malonic acid solution, stirs and evenly mixs, premixed after walnut oligopeptide 300g, Chinese yam polysaccharide 300g mixing
Material；Wherein, the dosage of malonic acid is the 50% of compound oligopeptide quality, and disperses malonic acid in suitable 70 DEG C of distilled water
It uses；
(2) be granulated, whole grain processing: the premix that step (1) is obtained is granulated whole grain, spray drying using spray granulation
200 DEG C of inlet temperature, 100 DEG C of outlet temperature, obtain solid particle；
(3) compressing tablet process: step (2) obtained solid particle is mixed with polyethylene glycol, is carried out compression molding, is obtained effervesce
Piece；Wherein, polyethylene glycol additive amount is the 10% of mass of solid particles, tableting pressure 1Mpa；
(4) packaging sterilizing: by effervescent tablet radiation sterilization, the irradiation dose of irradiation sterilization is 35kGy, packaging.
The effervescent tablet of the embodiment 2 preparation, in water disintegration time 150s, pH4.8, gas production 100mL, hardness 58N are crisp
Broken degree 0.4%, is dissolved in 200mL water, and solution is in faint yellow, clear, free from extraneous odour.
It improves sexual function measure of merit: this effervescent tablet solution being subjected to stomach-filling intervention to bull ICR mouse, is intervened
45d assesses its sexual function as intervention group；Blank group (stomach-filling equivalent aqua sterilisa), blank group bull ICR are set simultaneously
Mouse rides incubation period 1.67s, rides number 7 times, mates 2 times, and compound oligopeptide effervescent tablet intervention group, mouse ride latent
Volt phase 0.43s rides number 12 times, mates 4 times.
Antifatigue measure of merit: this effervescent tablet solution is subjected to stomach-filling intervention to bull ICR mouse, intervenes 30d, makees
For intervention group, its antifatigue effect is assessed, while blank group (stomach-filling equivalent aqua sterilisa) is set, comparison mice burden swimming power exhausts
Time, 5 ± 0.8min of naive mice, and compound oligopeptide effervescent tablet intervention group 9.25 ± 0.7min of mouse, antifatigue effect
Significantly.
Compound oligopeptide solid beverage embodiment 1
(1) premix is handled: oyster oligopeptide, walnut oligopeptide and Chinese yam polysaccharide are pressed following dosage: oyster oligopeptide
400g is pulverized after walnut oligopeptide 300g, Chinese yam polysaccharide 300g mixing, smashes it through 200 meshes；
(2) the compound oligopeptide powder after step (1) sieving the preparation of compound oligopeptide solution: is dissolved in 80 DEG C of hot water
In, it is sufficiently mixed uniformly, carries out high-pressure homogeneous, pressure 50MPa, number 1 time, obtain compound oligopeptide solution；
(3) concentration: by the resulting compound oligopeptide solution of step (2) using vacuum concentration, vacuum degree 1MPa, temperature
80 DEG C, obtain concentrate；
(4) dry: rapid (3) resulting concentrate being spray-dried, is spray-dried 120 DEG C of inlet temperature, outlet temperature
85 DEG C of degree；
(5) sterilization packaging: the solid powder radiation sterilization after step (4) are dried, irradiation dose 25kGy, packaging are
It can.
The solid beverage of the embodiment 1 preparation, can be quickly soluble in cold water or hot water, and for solution in faint yellow, clarification is saturating
It is bright, not stratified, free from extraneous odour.
It improves sexual function measure of merit: this solid beverage being subjected to stomach-filling intervention to bull ICR mouse, intervenes 45d,
As intervention group, its sexual function is assessed；It is arranged blank group (stomach-filling equivalent aqua sterilisa) simultaneously, blank group bull ICR mouse,
Incubation period 1.67s is ridden, is ridden number 7 times, is mated 2 times, and compound oligopeptide solid beverage intervention group, mouse ride incubation period
0.43s rides number 12 times, mates 4 times.
Antifatigue measure of merit: carrying out stomach-filling intervention to bull ICR mouse for this solid beverage, intervene 30d, as
Intervention group assesses its antifatigue effect, while blank group (stomach-filling equivalent aqua sterilisa) is arranged, when comparison mice burden swimming power exhausts
Between, 5 ± 0.8min of naive mice, and compound oligopeptide effervescent tablet intervention group 9.35 ± 0.7min of mouse, antifatigue effect are aobvious
It writes.
Compound oligopeptide solid beverage embodiment 2
(1) premix is handled: oyster oligopeptide, walnut oligopeptide and Chinese yam polysaccharide are pressed following dosage: oyster oligopeptide
650g is pulverized, smashed compound oligopeptide powder mistake after walnut oligopeptide 100g, Chinese yam polysaccharide 250g mixing
500 meshes；
(2) the compound oligopeptide powder after step (1) sieving the preparation of compound oligopeptide solution: is dissolved in 60 DEG C of hot water
In, it is sufficiently mixed uniformly, carries out high-pressure homogeneous；Pressure 100MPa, number 2 times；
(3) concentration: the resulting compound oligopeptide of step (2) is molten using vacuum concentration, vacuum degree 1MPa, temperature 70
℃；
(4) dry: rapid (3) resulting concentrate being spray-dried, is spray-dried 160 DEG C of inlet temperature, outlet temperature
85 DEG C of degree；
(5) sterilization packaging: by step (4) the dry compound oligopeptide powder radiation sterilization of gained, irradiation dose 35kGy,
Packaging.
The solid beverage of the embodiment 2 preparation, can be quickly soluble in cold water and hot water, and for solution in faint yellow, clarification is saturating
It is bright, not stratified, free from extraneous odour.
It improves sexual function measure of merit: this solid beverage being subjected to stomach-filling intervention to bull ICR mouse, intervenes 45d,
As intervention group, its sexual function is assessed；It is arranged blank group (stomach-filling equivalent aqua sterilisa) simultaneously, blank group bull ICR mouse,
Incubation period 1.67s is ridden, is ridden number 7 times, is mated 2 times, and compound oligopeptide solid beverage intervention group, mouse ride incubation period
0.3s rides number 13 times, mates 7 times.
Antifatigue measure of merit: carrying out stomach-filling intervention to bull ICR mouse for this solid beverage, intervene 30d, as
Intervention group assesses its antifatigue effect, while blank group (stomach-filling equivalent aqua sterilisa) is arranged, when comparison mice burden swimming power exhausts
Between, 5 ± 0.8min of naive mice, and compound oligopeptide effervescent tablet intervention group 8.15 ± 0.6min of mouse, antifatigue effect are aobvious
It writes.
Compound oligomeric peptide oral liquid embodiment 1
(1) preparation of compound oligopeptide solution: by oyster oligopeptide 150g, walnut oligopeptide 550g, Chinese yam polysaccharide 300g
It is dissolved in 5000mL distilled water, is sufficiently mixed, stir evenly；
(2) preparation of seasoning liquid: honey and citric acid are made an addition in compound oligopeptide solution, and honey additive amount is compound
The 10% of oligopeptide solution quality, citric acid additive amount is the 2% of compound oligopeptide solution quality, after being sufficiently stirred, using 200
Mesh filter-cloth filtering obtains seasoning liquid；
(3) homogenization: by the high-pressure homogeneous processing of the progress of seasoning liquid obtained by step (2), pressure 50MPa, temperature 50 C is secondary
Number 1 time；
(4) sterilization treatment: using high-temperature short-time sterilization for the seasoning liquid after rapid (3) homogenization, and temperature is 130 DEG C, when
Between be 30s；
(5) filling process: by step (4) sterilizing after seasoning liquid it is filling chlorine water disinfection container in finished product is made, fill
95 DEG C of temperature of dress.
Improve sexual function measure of merit: it is dry that the oral solution of the embodiment 1 preparation carries out stomach-filling to bull ICR mouse
In advance, intervene 45d as intervention group and assess its sexual function；It is arranged blank group (stomach-filling equivalent aqua sterilisa) simultaneously, blank group adult
Male ICR mouse rides incubation period 1.67s, rides number 7 times, mates 2 times, and compound oligomeric peptide oral liquid intervention group, mouse
Incubation period 0.45s is ridden, is ridden number 11 times, is mated 3 times.
Antifatigue measure of merit: this oral solution is subjected to stomach-filling intervention to bull ICR mouse, intervenes 30d, as dry
Pre- group, its antifatigue effect is assessed, while blank group (stomach-filling equivalent aqua sterilisa) is set, when comparison mice burden swimming power exhausts
Between, 5 ± 0.8min of naive mice, and compound oligopeptide effervescent tablet intervention group 8.22 ± 0.5min of mouse, antifatigue effect are aobvious
It writes.
Compound oligomeric peptide oral liquid embodiment 2
(1) preparation of compound oligopeptide solution: by oyster oligopeptide 650g, walnut oligopeptide 100g, Chinese yam polysaccharide 250g
It is dissolved in 5000mL distilled water, is sufficiently mixed, stir evenly；
(2) preparation of seasoning liquid: honey and citric acid are made an addition in compound oligopeptide solution, and honey additive amount is compound
The 5% of oligopeptide solution quality, citric acid additive amount are the 0.2% of compound oligopeptide solution quality, are sufficiently stirred, using 100
Mesh filter-cloth filtering obtains seasoning liquid；
(3) homogenization: carrying out high-pressure homogeneous processing for the resulting seasoning liquid of step (2), pressure 20MPa, and 40 DEG C of temperature,
Number 2 times；
(4) sterilization treatment: the rapid resulting seasoning liquid of (3) homogeneous is used into high-temperature short-time sterilization, temperature is 110 DEG C, the time
For 60s；
(5) filling process: the seasoning liquid after step (4) sterilizing is filling in the container of chlorine water disinfection, filling temperature 85
℃
It improves sexual function measure of merit: this solid beverage being subjected to stomach-filling intervention to bull ICR mouse, intervenes 45d,
As intervention group, its sexual function is assessed；It is arranged blank group (stomach-filling equivalent aqua sterilisa) simultaneously, blank group bull ICR mouse,
Incubation period 1.67s is ridden, is ridden number 7 times, is mated 2 times, and compound oligomeric peptide oral liquid intervention group, mouse ride incubation period
0.3s rides number 13 times, mates 7 times.
Antifatigue measure of merit: this oral solution is subjected to stomach-filling intervention to bull ICR mouse, intervenes 30d, as dry
Pre- group, its antifatigue effect is assessed, while blank group (stomach-filling equivalent aqua sterilisa) is set, when comparison mice burden swimming power exhausts
Between, 5 ± 0.8min of naive mice, and compound oligopeptide effervescent tablet intervention group 8.42 ± 0.8min of mouse, antifatigue effect are aobvious
It writes.
Next, to verify compound oligopeptide and its antifatigue effect of oral preparation, in conjunction with a variety of biochemistry means
It is further studied.
Effete test embodiment 1 --- improve sexual function effect test
Bull ICR mouse 12 is taken only to be randomly divided into intervention group and blank group (each 6), intervention group uses institute of the present invention
It states compound oligopeptide or oyster oligopeptide or walnut oligopeptide carries out stomach-filling intervention, specific practice are as follows: will be of the present invention compound
Oligopeptide or oyster oligopeptide or walnut oligopeptide are dissolved in suitable quantity of water by metering listed in table is made solution stomach-filling, intervenes
45d, blank group stomach-filling equivalent aqua sterilisa or without any processing, assesses its sexual function.
Mouse sexual organ index is tested first, statistical result is as shown in table 9 below, then each to mouse cavernous body
Biochemical indicator is detected, and statistical result is as shown in the following table 10 to 13, wherein testosterone measurement is counted using γ-radio-immunity
Device, NO measurement use kit measurement.
The influence of 9 oyster oligopeptide compatibility walnut oligopeptide of table and Chinese yam polysaccharide to mouse sexual organ index
Note: * indicates P < 0.01 compared with blank control group；
Influence of the 10 oyster oligopeptide of table to mouse cavernous body testosterone and NO content
Note: * indicates P < 0.05 compared with blank control group；
Influence of the 11 walnut oligopeptide of table to mouse cavernous body testosterone and NO content
Note: * indicates P < 0.05 compared with blank control group；
The influence of 12 oyster oligopeptide compatibility walnut oligopeptide of table and Chinese yam polysaccharide to mouse cavernous body testosterone and NO content
Note: * indicates P < 0.05 compared with blank control group；
The influence of 13 oyster oligopeptide compatibility walnut oligopeptide of table and Chinese yam polysaccharide to mouse cavernous body cGMP, PDE5 content
Note: * indicates P < 0.01 compared with blank control group；
To sum up, intervened 45 days using carrying out compound oligopeptide stomach-filling to Adult female ICR mouse, by number of copulations with
And sexual organ index is assessed, and the effect of compound oligopeptide improves sexual function is shown；Further, it shows compound oligomeric
Peptide can be improved serum NO level and testosterone concentration, cavernous body NO and cGMP level, reduces cavernous body PDE5 level, confirms in mechanism
The effect of sexual function can be improved in compound oligopeptide and its oral preparation.
Effete test embodiment 2 --- antifatigue effect test
Bull ICR mouse 12 is taken only to be randomly divided into intervention group and blank group (each 6), intervention group uses institute of the present invention
It states compound oligopeptide or oyster oligopeptide or walnut oligopeptide carries out stomach-filling intervention, specific practice are as follows: will be of the present invention compound
Oligopeptide or oyster oligopeptide or walnut oligopeptide are dissolved in suitable quantity of water by metering listed in table is made solution stomach-filling, intervenes
30d, blank group stomach-filling equivalent amount of water or without any processing assess its antifatigue effect.
Swimming with a load attached to the body test and statistical time are carried out to intervention group and naive mice first, then pass through full-automatic biochemical
Instrument detects mice serum biochemical indicator, kit measurement hepatic glycogen and muscle glycogen, and statistical result is as shown in table 14 to 19.
14 oyster peptide compatibility walnut oligopeptide of table and the evaluation of Chinese yam polysaccharide anti-fatigue effect
Note: * indicates P < 0.05 compared with blank control group；
The evaluation of 15 oyster oligopeptide anti-fatigue effect of table
Note: * indicates P < 0.05 compared with blank control group；
The evaluation of 16 walnut oligopeptide anti-fatigue effect of table
Note: * indicates P < 0.05 compared with blank control group；
17 oyster oligopeptide compatibility walnut oligopeptide of table and the evaluation of Chinese yam polysaccharide anti-fatigue effect
Note: * indicates P < 0.05 compared with blank control group；
The influence of 18 oyster oligopeptide compatibility walnut oligopeptide of table and Chinese yam polysaccharide to mice serum biochemical indicator
Note: * indicates P < 0.05 compared with blank control group；
The influence of 19 oyster oligopeptide compatibility walnut oligopeptide of table and Chinese yam polysaccharide to mouse hepatic glycogen muscle glycogen
Note: * indicates P < 0.05 compared with blank control group；
It is compound oligomeric using being carried out to Adult female ICR mouse further to verify the antifatigue effect of compound oligopeptide
Peptide stomach-filling is intervened 30 days, by exhausting the analysis of time to mice burden swimming power, shows the antifatigue effect of compound oligopeptide；
Further, showing compound oligopeptide can be improved lactic acid dehydrogenase activity, reduces blood lactase acid and serum urea nitrogen content, mentions
High muscle glycogen storage level confirms that compound oligopeptide and its oral preparation have antifatigue effect in mechanism.
It should be noted last that: technical solution of the present invention that the above embodiments are only illustrative and not limiting is any right
The equivalent replacement and do not depart from the modification of spirit and scope of the invention or locally replace that the present invention carries out, should all cover in this hair
Within bright protective scope of the claims.
Claims (9)
1. a kind of improve sexual function and antifatigue compound oligopeptide, which is characterized in that the compound oligopeptide is oligomeric with oyster
Peptide and walnut oligopeptide are primary raw material, are aided with Chinese yam polysaccharide and are prepared.
2. according to claim 1 improve sexual function and antifatigue compound oligopeptide, which is characterized in that compound with 1kg
Oligopeptide meter, dosage of each component are as follows: oyster oligopeptide 150-650g, walnut oligopeptide 100-550g, and surplus is that Chinese yam is more
Sugar.
3. according to claim 1 improve sexual function and antifatigue compound oligopeptide, which is characterized in that the oyster is low
Poly- peptide is the small molecule bioactive peptide extracted from oyster using biological enzymolysis technology, and relative molecular mass is less than 1000
Oligopeptide account for 90% or more.
4. according to claim 3 improve sexual function and antifatigue compound oligopeptide, which is characterized in that the biological enzyme
Enzyme used in solution technology is one in alkali protease, neutral proteinase, papain, trypsase and flavor protease
Kind.
5. according to claim 3 improve sexual function and antifatigue compound oligopeptide, which is characterized in that the oyster is low
Poly- peptide will also carry out flavoring processing after extracting, the flavoring processing be oyster oligopeptide after verifying separation by polymer into
Row embedding, polymer substance used are one of beta-cyclodextrin, maltodextrin, soybean protein isolate and N-LOK converted starch.
6. according to claim 1 improve sexual function and antifatigue compound oligopeptide, which is characterized in that the walnut is low
Poly- peptide is the small molecule bioactive peptide extracted from walnut using biological enzymolysis technology, and relative molecular mass is less than
1000 oligopeptide accounts for 80% or more.
7. according to claim 6 improve sexual function and antifatigue compound oligopeptide, which is characterized in that the biological enzyme
Solution technology uses stepwise discretization method, and successively carries out enzymolysis processing using alkali protease, neutral proteinase and flavor protease.
8. according to claim 6 improve sexual function and antifatigue compound oligopeptide, which is characterized in that the walnut is low
Before poly- peptide extracts, decortication processing and ungrease treatment are carried out to walnut in advance.
9. claim 1 to 8 is described in any item to improve sexual function and the oral preparation of antifatigue compound oligopeptide, feature
It is, the dosage form of the oral preparation includes effervescent tablet, solid beverage and oral solution.
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| CN112680492A (en) * | 2020-12-04 | 2021-04-20 | 广州天启生物科技有限公司 | Fishy smell-free low-molecular-weight oyster peptide and preparation method thereof |
| CN112741199A (en) * | 2019-10-29 | 2021-05-04 | 中国食品发酵工业研究院有限公司 | Oyster peptide with sexual function improving effect and preparation method thereof |
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