CN109007272A - Lactobacillus lactis feed addictive - Google Patents

Lactobacillus lactis feed addictive Download PDF

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Publication number
CN109007272A
CN109007272A CN201810511982.9A CN201810511982A CN109007272A CN 109007272 A CN109007272 A CN 109007272A CN 201810511982 A CN201810511982 A CN 201810511982A CN 109007272 A CN109007272 A CN 109007272A
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freeze
dried powder
powder
sea
blackcurrant
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张奎昌
张志年
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Jiangsu Qianyaotang Traditional Chinese Medicine Research Institute Co Ltd
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Jiangsu Qianyaotang Traditional Chinese Medicine Research Institute Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/24Compounds of alkaline earth metals, e.g. magnesium
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00Medicinal preparations containing inorganic active ingredients
    • A61K33/44Elemental carbon, e.g. charcoal, carbon black
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56Materials from animals other than mammals
    • A61K35/57Birds; Materials from birds, e.g. eggs, feathers, egg white, egg yolk or endothelium corneum gigeriae galli
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/12Antidiarrhoeals
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00General protective or antinoxious agents
    • A61P39/02Antidotes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/157Lactis

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  • Animal Behavior & Ethology (AREA)
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  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Fodder In General (AREA)

Abstract

Lactobacillus lactis feed addictive disclosed by the invention, it is characterized in that being uniformly mixed by lactobacillus lactis, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, the charing egg-shell meal for adding surplus again is uniformly mixed, composite packaging is made: lactobacillus lactis 30000 hundred million -150000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 5-50g, Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-dried powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-150g, remaining is charing egg-shell meal.Viable bacteria contained by the present invention is clear, application and Quantitatively Selecting conducive to user, using sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder and charing egg-shell meal as the matrix of additive, stability, trophism and the adsorptivity of additive institute mycetome can not only be improved, the nutritive value for promoting product simultaneously promotes the metabolism of animal, improves body resistance against diseases, promote growth of animal, the final economic benefit for improving aquaculture.

Description

Lactobacillus lactis feed addictive
Technical field
The invention belongs to feed additive fields, are related to a kind of for cultivated animals probiotic feed additive.Specifically It is related to a kind of lactobacillus lactis feed addictive.
Background technique
The lactic acid bacteria that lactic acid bacteria (lactic acid bacteria, LAB) is currently known has more than 100 kind.Lactic acid bacteria Applicating history is very long, such as the production and production of dairy products, pickles, ensilage.In the enteron aisle of humans and animals there is A large amount of lactic acid bacteria.Lactobacillus preparation is a kind of preparation containing live lactobacillus, entitled alctasin.Lactic acid bacteria grows in enteron aisle Development decomposes carbohydrate and generates lactic acid and form acidic environment, to inhibit the proliferation of harmful bacteria in intestinal canal, and prevent from producing poison and It causes a disease.The strain for preparing lactobacillus preparation has lactobacillus acidophilus, lactobacillus bulgaricus, streptococcus lactis and streptococcus fecalis etc..
Lactobacillus is the normal settler of animal (especially poultry) enteron aisle.Poultry went out after shell in a few hours can be Streptococcus fecalis, enterobacteria (Enterobateria) and the clostridium (Clostridia) that discovery is dispersed in enteron aisle, with Bacillus acidi lactici just starts to settle down in alimentary canal in a couple of days afterwards.Lactobacillus is the main Pseudomonas of fowl piperazine capsule flora, with large intestine Bacillus, streptococcus (Streptococi), which are compared, occupies advantage.Bacillus acidi lactici is adhered to fowl prolixity capsular epithelium, and this adhesion has kind Specificity.Fowl piperazine capsule Bacillus acidi lactici is the Bacillus acidi lactici source that poultry maintains intestinal bacteria balance.Bacillus acidi lactici is maintaining to digest The aspect of stablizing of the normal microflora system in road plays conclusive effect.Most probiotic products be Lactobacillus or other Lactic acid-producing bacteria.
Lactic acid bacteria anaerobism or amphimicrobian growth, still are able to survive under pH3.0-4.5 acid condition.It is in animal intestinal tract Middle by monosaccharide, especially Lactose conversion is lactic acid, to reduce intestinal pH, prevents foreign bacteria in the field planting of enteron aisle, inhibits large intestine The growth of the pathogens such as bacillus, detection of Salmonella, and the growth of final lactic acid bacteria inhibiting itself.Lactic acid bacteria also can be by adjusting enteron aisle PH promotes gastrointestinal peristalsis to activate pepsin, helps the digestion of food, absorbs, mitigate flatulence and promote liver function etc.. Lactic acid bacteria inhibits the growth of pathogen by competitive exclusion effect (competitive binding site).Lactic acid bacteria can also stimulate siberian crabapple System improves immunity of organisms, improves intestinal tissue to the resistivity of bacteria attack.Some Bacillus acidi lacticis can neutralize certain toxin, As lactobacillus bulgaricus can neutralize escherichia coli enterotoxin, but precise mechanism is unclear.Bacillus acidi lactici also can produce itself Some digestive ferments, such as invertase, lactase and peptase, help to improve animal digestion function.Sawada report, certain lactic acid Contain a kind of safe and effective natural anti-hypertension compound SG-1 in bacilli-cell wall.Lang Zhongwu is equal to 1994 in egg young bird Brood of layers chichen is added in daily grain of chicken, as a result feed efficiency improves 8.73%-11.23%;Xiao Zhenfeng etc. (1995) examination Verify bright, Brood of layers chichen is compared compared with antibiotic control group, improves feed efficiency 4.6%.Furthermore Bacillus acidi lactici can The digestive function of small intestine is improved by increasing surface area.Animal is in stress be (as birth, wean, daily ration variation, intestinal pH are abnormal Increase) during, total variation tendency is that pathogen is increased, and Bacillus acidi lactici quantity is reduced.Lactobacillus is successfully established in enteron aisle PH can be made to become stable.
The characteristics of lactic acid bacteria are as follows: be 1. the main fungal component of many animals alimentary canal, normal flora can be formed: 2. in micro- need Lactic acid is generated under oxygen or anaerobic condition;3. there is stronger acid resistance;4. generating a kind of bacteriocin
(Bacteriocin), hay bacillus, staphylococcic growth can effectively be inhibited.The shortcomings that lactic acid bacteria is that temperature capacity is poor, Dead under the conditions of 65 ~ 75 DEG C, instantaneous high-temperature can be killed and be failed during feed granules.The type of preparation of such bacterium It is various, using earliest, most extensively.
Lactobacillus lactis (Lactobacillus Lactis) is also known as German-style Lactobacillus lactate subspecies, is Minister Agriculture of China's public affairs " feed addictive catalogue (2013) " for accusing No. 2045 announcement are allowed for the microorganism of cultivated animals, with regard to current lactic acid cream bar The dosage form that bacterium uses as additive has aqua and pulvis two major classes, and aqueous solution dosage form is because in thallus aqua easy to use Nutritional substance continues to be metabolized, and growth fermentation, therefore, the thallus of this dosage form plays pendulum, and storage life is shorter.Pulvis Dosage form is then that thallus is dehydrated at a slow speed the thallus lived to using drying means such as low temperature hot wind, freezings, and thallus is made to be in steady Fixed drying dormant state recycles substance that can be feeding to be uniformly mixed as filler and relatively stable powder-type is made Biological feed additive.But with regard to current manufacturing enterprise in view of raw material be easy to get and processing cost, often select rice bran, wheat bran, One of corn flour, maize cob meal, peanut shell powder, conch meal, vermiculite power, mountain flour, calcium carbonate, medical stone, zeolite powder are several Kind mixture, some also use plant straw powder or grass meal as filler material.The considerations of these are in line with production cost, then have ignored The stability of microbial bacteria and the trophism value of feed addictive.
In order in use, have certain adhesion, microbial bacteria feed addictive be made to exist in microbial bacteria additive It admixes when being mixed in animal feed, can be adsorbed on animal feed, convenient for the uniformity in animal feeding and be fed into bacterium amount Maximization, some products take pains a certain amount of binder of addition.
In order to make to raise the relative constant of animal stomach inner pH value, acid is continued up to after so that the foodstuff being fed into is entered small intestine The effect of change, in the product often simultaneously using acidulants such as citric acid, lactic acid, hydrochloric acid, fumaric acid, but these acidulants are not only straight It connects and locally causes acidity too strong microbial bacteria contained by additive, influence the survival of thallus, meanwhile, these acidifying substances are to cultivation Animal also without the nutritive value directly utilized, meanwhile, there is very big irritation to the gastrointestinal tract of the cubs of livestock and poultry, chick, Compatibility is using not ideal enough.
Above-mentioned deficiency can be overcome by developing one kind, can either keep stability, the trophism of institute's mycetome, be had again to thallus There is adhesion, while having again to the filler used and providing the additive for microbe feedstuff of nutritive value to cultivated animals, is Promote the basis of feed additive industry long-term stability development.
Summary of the invention
The purpose of the present invention is to provide a kind of new single type lactobacillus lactis feed addictive, which is raised Not only there is feed additives thallus to stablize, and the thallus time-to-live is long, and used auxiliary agents have certain adhesiveness And adsorptivity, thallus can be made preferably to be bonding on one with auxiliary agents, meanwhile, complementary additive has certain acid Property can not only provide nutriment abundant after animal feeding, while to be able to maintain stomach inner pH value relatively permanent for its acidic materials It is fixed, into small intestine after the effect of continuing up to acidification.The purpose is to provide the lactic acid cream bar of a kind of more stability and trophism Bacterium feed addictive.
Lactobacillus lactis feed addictive of the present invention, it is characterized in that by lactobacillus lactis and a certain amount of seabuckthorn fruit powder, Rosa roxburghii Tratt Powder, wintercherry powder, blackcurrant powder, cactus powder and charing egg-shell meal uniformly mix, and obtain the new lactobacillus lactis feed of one kind and add Add agent, the thallus of this additive is stablized, and the thallus time-to-live is long, and seabuckthorn fruit powder, Ribes burejense powder, wintercherry powder, blackcurrant powder are containing sugar Amount is high, and especially cactus powder all has preferable Adhesion property, and charing egg-shell meal also has preferable adsorptivity.It is wherein husky Spine, Rosa roxburghii Tratt, wintercherry, blackcurrant are the ascorbic fruits of high-content, and acidity is extremely strong, are not only provided to cultivated animals abundant The nutriments such as vitamin, the acidic materials after feeding play the effect for promoting digestion well, to highlight this The purpose of invention.
In order to realize the present invention, the following technical solutions are specifically adopted:
Lactobacillus lactis feed addictive, feature it be first by lactobacillus lactis, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, acid It starches freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder to be uniformly mixed, then adds the charing egg-shell meal of surplus and be uniformly mixed, it is multiple It closes packaging to be made: lactobacillus lactis 30000 hundred million -150000 hundred million, sea-buckthorn freeze-dried powder 5- in every 1000g feed addictive 50g, Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-dried powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-150g, Remaining is charing egg-shell meal.
Lactobacillus lactis described above is lactobacillus lactis dry powder product.
The lactobacillus lactis dry powder product, can be used it is commercially available be used as every gram of feed addictive it is dense containing viable bacteria The weight proportion of the preparation that degree is no less than 250 hundred million, the lactobacillus lactis dry powder product and the filler material in formula is by bacterium The thallus content of soma powder and final products determines.
The lactobacillus lactis dry powder product also can use the culture medium that existing method selection accordingly optimizes and pass through liquid Then body Zymolysis Equipment culture is separated the thallus in fermentation liquid with high speed centrifugation mode, be placed in dry indoor cold wind Or bacterium mud drying is made with vacuum freeze drying mode, every gram of viable bacteria concentration no less than 250 hundred million thallus of acquisition are dry Powder product.
Sea-buckthorn freeze-dried powder described above, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, It being made by following methods, technical process is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove carpopodium respectively, The impurity such as rotten fruit, rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step;Rinsing condition described above is With the citric acid of 40 DEG C of water addition weight 0.1%, 15min is rinsed, and be stirred continuously, pulls out to be put into pure water and rinse 3 times;On Stating freezing is that will be cooled to rapidly -45 DEG C or less after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively;Above-mentioned distillation Dry process conditions are temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dry until moisture is less than 6%/w;It is above-mentioned Low-temperature grinding is the crushing that temperature of charge is in 0 DEG C or less progress, 100 mesh of grinding particle size >.
Composite packaging described above uses interior outer bag composite packaging, and inner bag uses polypropylene plastics pocket, and outer packing uses aluminium Foil bag is filled with silica dehydrator pouch between interior outer bag.
Charing egg-shell meal described above is made by following methods:
(1) dry, dipping first cleans up eggshell, dries moisture, is dried 1-2 hours, then pulverizes into advance 20-30 mesh coarse powder sprays the salt (sodium chloride) that prepared weight fraction is 25% by weight 1:0.5 on eggshell coarse powder Solution is mixed in sprinkling, is placed after mixing 6-7 hours, then uniformly mixing is primary again, then places 6-7 hours;The purpose is to Salt is allowed effectively to penetrate into eggshell organization internal gap;
(2) charing impregnated eggshell powder raw material is put into rotary carbonizing pot, 420-450 DEG C at a temperature of rotate charcoal Change 70-80 minutes, (carbonization process there should be corresponding scraping blade, to prevent raw material in carbonization process until the observation complete blackening of raw material Viscous pot and agglomeration);
(3) raw material carbonized is rapidly moved in activation furnace by activation, is closed fire door, is passed through carbon dioxide gas, is caught up with to the greatest extent wherein Air, at 720-800 DEG C anoxybiotic activate 55-85 minute (corresponding turning mechanism is set in activation furnace, make activation raw material it is every It can be stirred within 10 minutes once, to guarantee activation fully and uniformly);
(4) after activated material is taken out in cleaning, it is allowed to cool room temperature, activated material is gradually rinsed in rinse bath with clear water, until Until basic inspection does not measure sodium chloride;After having cleaned sodium chloride, it is small that activated material 2.5-3 is submerged with the hydrochloric acid that mass ratio is 12% When, after impregnating, with 95 DEG C of pure water washing activated materials, until material grain is there is no after white, then with the drift of room temperature pure water It is washed till neutral (pH value is about in 6.9-7.1), filtering obtains rinse activator material;
(5) dry and screening, which dries the activated material of rinsed clean at 115-120 DEG C to water content, is lower than 6%(w), cross 60 Mesh, the coarse grain after screening are pulverized again, are made all by 60 meshes to get charing egg-shell meal product.
Drying described above uses heated-air drying or far-infrared ray drying;Dry condition is 180 DEG C -300 DEG C.It is dried Purpose be that can make the crisp matter of eggshell, increase eggshell hole be conducive to sprinkling saline solution effectively penetrate eggshell internal voids In.
Eggshell described above is the mixed of any one or a few in egg shell, Ovum Anas domestica putamina, goose egg-shell and quail eggshell Close eggshell.
The prioritization scheme of lactobacillus lactis feed addictive of the present invention is, first by lactobacillus lactis, sea-buckthorn freeze-dried powder, thorn Pears freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add the charing egg-shell meal of surplus It is uniformly mixed, composite packaging is made: lactobacillus lactis 50000 hundred million -150000 hundred million in every 1000g feed addictive, husky Spine freeze-dried powder 10-50g, Rosa roxburghii freeze-dried powder 10-50g, wintercherry freeze-dried powder 5-30g, blackcurrant freeze-dried powder 5-30g, cactus freeze-drying Powder 70-150g, remaining is charing egg-shell meal.
The further prioritization scheme of the present invention is lactobacillus lactis feed addictive, it is characterized in that first by lactic acid cream bar Bacterium, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add The charing egg-shell meal of surplus is uniformly mixed, and composite packaging is made: the lactobacillus lactis 70000 in every 1000g feed addictive Hundred million -150000 hundred million, sea-buckthorn freeze-dried powder 15-50g, Rosa roxburghii freeze-dried powder 15-50g, wintercherry freeze-dried powder 8-30g, blackcurrant freeze-dried powder 8-30g, cactus freeze-dried powder 85-150g, remaining is charing egg-shell meal.
Further optimization plan of the present invention is lactobacillus lactis feed addictive, it is characterized in that first by lactic acid cream Bacillus, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder are uniformly mixed, then add The charing egg-shell meal of surplus is added to be uniformly mixed, composite packaging is made: the lactobacillus lactis 90000 in every 1000g feed addictive A hundred million -150000 hundred million, sea-buckthorn freeze-dried powder 20-50g, Rosa roxburghii freeze-dried powder 20-50g, wintercherry freeze-dried powder 10-30g, blackcurrant freeze-drying Powder 10-30g, cactus freeze-dried powder 100-150g, remaining is charing egg-shell meal.
Lactobacillus preparation is more and more as the research report of feed addictive in recent years, is related to face also more and more extensively, from Pig, chicken to ox recycle from production performance, poultry house enhancement of environment, feces of livestock and poultry to various aspects such as livestock and poultry pestilence prevention and treatments, several Cover whole fields of animal productiong.
Some researches show that add daily gain and feed that lactobacillus preparation is remarkably improved chick in Broiler Diet Conversion ratio.Beneficial microbe bacterium is added in piglet, chick, laying hen and broiler chicken feed by Pu member flood, and gaining effect is obvious, Piglet through feeding can increase weight 13.2% than control group every, and chick survival rate improves 17.80%.An Yongyi meat in 4-7 weeks With Lemonal is added in chick fodder, discovery reduces the Broiler chicks death rate to some extent.
Lactic acid bacteria can effectively prevent the generation of livestock and poultry, and test result confirms, prevent large intestine using probiotics The effect of angstrom uncommon bacterium infection is significantly better than chemoprophylaxis.Thus suggest, produce in practice, can be taken with probiotics in poultry husbandry The infection of pre- bacteriological protection is used for for drug, this can not only to obtain ideal preventive effect, and can also overcome chemoprophylaxis band The many negative effects come.
The effect of improving aquaculture is added beneficial micro- to inhibit pond culture harmful microbe proliferation to pond Biological agent can inhibit harmful microbe proliferation, to achieve the effect that containing.Since lactic acid bacteria class beneficial bacterium can generate Content of material adjusts the internal and external environment of aquatic animal, has anti-antagonism and antioxidation, to increase aquatic animal Premunition.Microbe bacteria preparation not only increases the yield of fish in experiment pool, and reduces the morbidity of fish, shows micro- Biological agent promote cultivation object growth and development, improve water environment, in terms of have broad application prospects. It is demonstrated experimentally that microorganism formulation have coexisted with host, keep enough bacterial numbers and vigor, can be special as dominant bacteria etc. Point.
The feeding effect of microorganism inoculant, the feeding effect in relation to lactic acid bacteria class microorganism inoculant.Report both at home and abroad Compare more, be all generally speaking positive-effect, has and improve speed of weight increment, improve efficiency of feed utilization, prevent and cure diseases, reduce death Rate, the effect finally increased economic efficiency.As for the big problem of using effect amplitude of variation, mainly due in production and storage Quality control method is incapable caused, and the living cells quantity as contained in product is few, or contains microbe species and label institute Column are not inconsistent.It causes in the factor for influencing microorganism inoculant feeding effect.
Currently, most of its bacterial strain of microorganism fodder bacteria preparation compositions, colonization ability of bacterial strain etc. are influenced using effect The factor of fruit.Key using active ingredient is after guaranteeing that these microorganisms are mixed into feed, to be able to maintain its active extract and extremely adopted Food enters animal intestinal tract, and breeds in corresponding enteron aisle spots localization.In cultivation, the application of microorganism formulation, some effects are shown It writes, some is then not satisfactory, or even can't see positive effect, the not outer factor for having following several respects of tracing it to its cause.
(1) strain
Whether excellent ignored as the strain of microbial bacteria extremely, appearing not to be any seed can increase production equally, therefore strain Source and screen it is particularly significant.U.S. FDA, which defines more than 40 kinds of bacterium, all can serve as the selection strain of probiotics, China The bacterial strain of Ministry of Agriculture's official approval production mainly has bacillus, lactic acid bacteria, fecal bacteria, thermophilic bacterium to eat into lonely bacterium and saccharomycete etc., rule These fixed strains can only be prompted to people and can use or permit using, but are applied to methods and applications effect and still have factors It needs to consider.
(2) viable bacteria content
Viable bacteria content is the key that product quality.Microorganism inoculant must contain a certain amount of viable bacteria, can be only achieved expected effect Fruit, it is certain it is noted that dose-effect relationship in microbe bacteria preparation.
(3) stability of preparation
Microorganism inoculant is with the extension of time, number of viable gradually decreases, it means that its effect gradually decreases.How to protect The stability for holding viable bacteria in probiotics is to develop and produce the practical problem faced, and the factor for influencing stability mainly has temperature Degree, humidity, acidity, machinery, nutrition etc..
(4) timeliness is applied
According to microbe bacteria preparation to the clinical prevention experience of animal, microorganism inoculant early stage, juvenile stage, stress the phase Application effect is most obvious, in early days application refer to animal birth after and stress phase early application, so that beneficial microbe is first impressions are strongest, shape At dominant microflora.
It (5) is suitable for metering
The effect of animal applications microorganism inoculant is only reached certain by being realized due to microorganism administration dosage Amount, and the regular hour is maintained, flora advantage could be formed, ecological effect is obtained.In order to guarantee that beneficial bacterium can play work With suitable additive amount is important, and additive amount is insufficient or excessive all to be can not achieve the desired results.Additive amount deficiency cannot reach Desired effect, excessive addition can only make weight gain and food conversion be deteriorated, feed cost caused to rise, because microorganism itself Breeding is also required to consumption energy, and excessive microbes fight for nutriment with animal itself.
(6) specific aim is applied
Poultry microorganism formulation some is used as feed addictive, and some is used for disease preventing and treating, and purposes is different, strain and recipe ratio Example is different.The purpose that microorganism inoculant is used as feed addictive is to promote growth, improves efficiency of feed utilization, is mostly used gemma bar Bacterium, saccharomycete etc. are made;Microorganism formulation for preventing disease is mostly used strong beneficial of the acid producing abilities such as Bacillus acidi lactici, enterococcus Microorganism is made.The microorganism of animal species and animal physiological state is generally lactic acid bacteria, bacillus, saccharomycete etc., and fits What it is for ruminant is fungi and yeasts etc..
For the feeding effect and influence factor of microorganism formulation, lactobacillus lactis feed addictive of the invention is made every effort to The preparation of strain single type buys the definite guarantee for making every effort to number of viable to strain, clearly quantifies to invention product viable bacteria content, Guarantee that the product provided can guarantee the standard of its viable bacteria content, bacterium contained by the product for enabling producer clearly to select Kind, while but also its clear viable bacteria content, accomplishes purposefully to select and apply.In order to reduce the viable bacteria loss of product, to institute With the selection of adjuvant excipient, the trophism of animal feeding is considered not only, while in view of to lactobacillus lactis contained by it Trophism and stability.In order to realize product in early stage, juvenile stage application effect, therefore, inventive article is selected complementary Raw material had both considered the nutritional need of young animal, while in view of product is to the palatability of young animal, therefore, the present invention The complementary raw material that valence is low, trophism is poor, palatability is poor, difference is absorbed and utilized are not used.
In order to allow producer to pass through the specific aim of selection microbial bacteria feed addictive clear optimal dose and application A large amount of investigation to market, most owner thirst for buying single type additive for microbe feedstuff, make it possible to independently determine suitable Suitable dosage and determine that it targetedly applies cultivated animals according to their own needs.Thus, a kind of single type of the application The exploitation of lactobacillus lactis feed addictive, substantially overcomes the deficiencies in the prior art, has substantially met the demand of user.
Sea-buckthorn (Hippophae rhamnoides Linn.), fruit nutriment rich in and biological active matter Matter can be widely applied to many fields of the national economy such as food, medicine, light industry, space flight, agriculture and animal husbandry fishery.Fructus hippophae and oil With very high medical value, there is a cough-relieving apophlegmatic, relieving dyspepsia, promoting blood circulation to remove blood stasis effect, can treat gastric duodenal ulcer with And indigestion etc..
Sea buckthorn fruit is full of nutrition, according to surveying and determination in its fruit containing multivitamin, fatty acid, microelement, sub- oil element, The various amino acid of Hippophate flavone, superoxides isoreactivity substance and needed by human body.Wherein Vitamin C content is high, and every 100 grams In fruit juice, Vitamin C content can reach 825-1100 milligrams, is 2-3 times of Kiwi berry, is known as the laudatory title of the king of vitamin C.Contain Sugared 7.5%-10%, the 3%-5% containing acid.
Rosa roxburghii Tratt (Rosa roxbunghii) is fruit also known as the mountain king fruit, thorn of the perennial machaka rosa roxburghii of rosaceae Certain kind of berries fruit, thatch pears etc..With clearing heat and promoting fluid, stomach strengthening and digestion promoting, relieving summer-heat and other effects.Rosa roxburghii Tratt vitamin B rich in, C, carrotene, The ingredients such as malic acid, citric acid are usually used in deficiency of stomach-Yin, appetite stimulator, indigestion or eating accumulation, glutted full bored, diarrhea Loose stool;Pyreticosis or hot summer weather hurt saliva, thirsty, scanty drak urine.
Mature Rosa roxburghii Tratt is fleshy hypertrophy, sweet and sour, fruit are rich in sugar, vitamin, carrotene, organic acid and more than 20 kinds of ammonia More than base acid, 10 microelement and superoxide dismutase, especially Vitamin C content beneficial to human body are high, are to work as It is highest in preceding fruit, it is 50 times of citrus 841.58 milligrams -3541.13 milligrams of content in every 100 grams of fresh fruits, Kiwi berry 10 times, the laudatory title with " king of vitamin C ".Fruit of Grossularia burejensis Berger has very high nutritive value and medical value, sour, puckery, flat, Digestion-promoting spleen-invigorating restrains stopping leak, for treating having indigestion abdominal distension, dysentery, enteritis, vitamin C deficiency etc..
Wintercherry (Physali alkekengiL.) also known as groundcherry, franchet groundcherry persistent calyx or fruit, cape gooseberry etc..Containing more in Fruit of Physalis Kind of nutritional ingredient, wherein the content of calcium be 73.1 times of tomato, 13.8 times of carrot, ascorbic content is tomato 6.4 times, 5.4 times of carrot.With unique flavor and nutrition abundant.Fruit of Physalis aromatic flavour, delicious.Berry is rich Containing vitamin C, beta carotene, more than 20 kinds of mineral elements and 18 kinds of amino acid, there is heat-clearing, removing toxic substances, diuresis effect is usually used in Control heat-syndrome cough, pharyngalgia, jaundice, dysentery, in water, the treatment of furunculosis, erysipelas etc..
Blackcurrant scientific name elements of black currant (Ribes nigrum L.) also known as currant, purple plum etc., ripening fruits are black Small berries, it is interior to be rich in vitamin C, anthocyanidin.Blackcurrant is not only a kind of delicious flavour, fruit full of nutrition, and has Good medicinal efficacy.Blackcurrant fruit is rich in mineral matter element, and the Zn content in mineral matter element is high, can be used as and comes for zinc Source;Ascorbic content is high, hence it is evident that is higher than citrus, hawthorn, also contains phosphorus, magnesium, potassium, calcium, anthocyanidin, phenolic substances.Seed Linoleic acid content is high.Ascorbic content is very rich in blackcurrant, it is often more important that is also melted into containing a large amount of antioxygen Point, this assures ascorbic function and effect.Blackcurrant is rich in such as anthocyanidin, vitamin C, flavones, Quercetin, red bayberry The substances such as alcohol, phenolic acid, catechin and blackcurrant polysaccharide, these are all the bioactive substances with good anti-oxidation function.
Cactus (scientific name: Opuntia stricta (Haw.) Haw.var.dillenii (Ker-Gawl.) Benson), It is a kind of plant of Opuntia.Alias celestial being palm, kwan-yin palm etc..Cactus is the meat shrub that grows thickly, the wide ovum of top branch Shape, obovate elliptic or subcircular;Flower spoke shape, holder obovate.It is used as medicine with complete stool.The four seasons can adopt.
Cactus is cool in nature, sour, nontoxic.Have the promoting flow of qi and blood circulation, heat-clearing toxin-expelling functions.Mainly have clearing heat and detoxicating, eliminating stasis to subdue swelling, The functions such as the promoting flow of qi and blood circulation, benefiting stomach and stopping pain.Cactus contains amino acid and various trace elements in needed by human 8, and embraces green The preciousness ingredient such as lotus, Kok Beattie cents, cholla stem, starches through cactus crude drug and analyzes, contain pectin, polysaccharide and glue exudate.From herb point From extracting to obtain freidelin ketone (friedelin), -3 α -ol of freidelin (friedelan-3- α-ol), taraxerone (taraxerone) and taraxerol (taraxerol).
Eggshell, quantity is biggish in China's poultry farming chicken, duck, goose and quail etc., using egg, duck's egg, As in food processing process, the useless eggshell enormous amount generated is provided according to existing disclosed report for goose egg and quail egg Material display, the eggshell total amount that a medium-sized city is monthly discarded reach 60-100 tons, and account for egg size by Eggshell weight 11% calculates, The eggshell that about 1,500,000 tons of the daily output in China, not only pollutes environment, but also waste of resource in this way.Discarded eggshell is integrated It utilizes, resource utilization can not only be improved and avoids environmental pollution, and be greatly improved the economic benefit and social benefit of enterprise.
Wherein egg shell is in China's yield maximum, and egg shell accounts for the 10-12% in entire egg, by under film, shell on shell Film and eggshell three parts composition, eggshell is a kind of calcareous hard shell, is located on shell between film and shell lower film.In the change of egg shell In studying point, minerals account for 95%, and protein 3.3%, moisture 1%, fat 0.03%, remaining is carbohydrate and other have Machine substance (not including certainly, a large amount of remaining egg white in eggshell in the above ingredient).Eggshell mineral ingredient is mainly Calcium carbonate accounts for 94% or so, remaining is magnesium carbonate and calcium phosphate, and calcium, phosphorus content account for 34.9% and of eggshell dry matter respectively 0.2%.In eggshell, seven kinds of vitamins and various trace elements and some important enzymes, peptide matters can be measured, he Although content seldom, be all the substance needed in human body or animal body.
In the prior art, it is replenished the calcium with egg-shell meal, has the product of the grinds after sterilizing by eggshell in people's product, also have The application of organic calcium is prepared into using primary or secondary clacining method.It is at present most widely in drug, food and nutriment In preparation, micron order, submicron order and nano level superfine powder are mostly used, is added to various food, nutrition kind, has reached very Good effect.Application of the eggshell in China's animal-breeding is taken mostly by eggshell cleaning, drying, and milling is added into feed The method of feeding, since grinding particle size is larger, being absorbed and utilized for animal is poor, can't reach good application effect.
The present invention is to clean eggshell, after high temperature drying, then be made one through spice, charing, activation, cleaning, drying, screening The adsorbent of kind function admirable, the program not only remains the nutrition that eggshell utilizes, while making the abundant carbonaceous of eggshell, not only right It is contained it is calcareous form organic calcium, and form the filling substrate with adsorption capacity.
The beneficial effects of the present invention are:
(1) product of the present invention can highlight the viable bacteria content of lactobacillus lactis, significantly improve thallus in closed composite packaging Survival rate, through batches of products reserved sample observing 12 months at room temperature, lactobacillus lactis average viability is and right 95.3% It is 83.7% according to the lactobacillus lactis survival rate in product (filler of reference substance is wheat bran);Product of the present invention is single type lactic acid Lactobacillus feed addictive can provide the selection of the specific aim and quantization with application to producer, have conducive to livestock and poultry Targetedly it is easy to use.
(2) pass through sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder and cream Lactobacillus lactis mixes, can be preferably due to the stickum in the colloidal substance that these dry powder formulations are rich in, especially cactus The adhesive effect between additive and feed is increased, and improves the utilization rate of additive;
(3) by sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder high-content amino acid, vitamin, mineral element, more Sugar.The nutritional ingredients such as the vitamin C of organic acid, especially high-content and various bioactivators, to raising animal immune energy Power is simultaneously obviously improved meat, Egg Quality, the acidic materials in especially above-mentioned dry powder, utilization, promotion animal to contained microorganism Digestion all has significant effect;Since sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant are rich in oligosaccharide kind polysaccharide, because of oligosaccharide kind carbon hydrate Object can effectively stimulate the proliferation of intestinal beneficial microbes, harmful bacteria can be blocked to stick intestinal mucosa.Polysaccharide therein can also Enhance the pharmacological action and ecological effect of probiotics.
(4) due to using Freeze Drying Technique means, make sea-buckthorn obtained, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-drying Powder can maximumlly remain its active constituent, have the smell that distinctive sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus are applied And flavour, free from extraneous odour, add the preference of animal.
(5) eggshell is first subjected to drying and other treatment through high temperature using charing egg-shell meal, further through spice, charing, activation, clear It washes, dry and sieves, form a kind of excellent adsorbent, which not only forms absorption to the saccharomycete in product Power, while making the eggshell after charing that still there is the organic calcium effect of activation, meanwhile, after animal feeding, have well to stomach The toxin of enteron aisle has suction-operated, has stop dysentery, antidiarrheal effect for the animal of diarrhea and diarrhea, has good active carbon Medicinal effects.
(6) preparation in the present invention to sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus freeze-dried powder is not removed the peel, deseeding step Suddenly, it is therefore an objective to by being crushed after freeze-drying, so that its skin and seed is worn into fine powder, make anthocyanidin, oil in its skin and seed Class, organic acid, bud fragrance substance are retained, and the nutritive value of its freeze-dried powder is promoted.
(7) present invention is lyophilized using sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus Powder, matrix of the charing egg-shell meal as additive contain constants such as a large amount of calcium, phosphorus, potassium, sodium, magnesium, zinc and micro in matrix Element, vitamin C still rich in, anthocyanidin, beta carotene, malic acid, citric acid, protein, amino acid, nucleic acid, Portugal Grape sugar, lipoidis, carbohydrate, B family vitamin such as B1、B2、B6、B12And the physiological activity such as folic acid, niacin, pantothenic acid, inositol Substance, not only improves the nutritive value of lactobacillus lactis additive, while promoting the metabolism of animal, improves the disease-resistant of body Ability promotes growth of animal, finally improves the economic benefit of its aquaculture.
(8) product of the present invention uses interior outer bag composite packaging, and interior outer bag interlayer is filled with silica-gel desiccant, and product does not return Tide, no agglomeration, product is loose, good fluidity, can preferably keep exterior quality and inherent quality.
Operation to not having its technical process stated in detail in the present invention is in the common sense of those skilled in the art Operation.With regard to the purchase and storage of heretofore described sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant fresh fruit and fresh cactus, the present invention The construction of people's unit has 20,000 tons of freezer facility, has enough guarantees to storage fresh fruit material.
Specific embodiment
Embodiment 1
The preparation of sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder:
Sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, the cactus for taking fresh mature respectively remove carpopodium, the impurity such as rotten fruit, with containing heavy 40 DEG C of aqueous solutions of 0.1% citric acid are measured, 15min is rinsed under constant stirring and pulls out, be put into pure water and rinse 3 times, pull out It is drained;It will be moved into refrigerator after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively, be cooled to -45 DEG C rapidly Below;Respectively by the sea-buckthorn freezed, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus move into lyophilization machine in, sublimation temperature control- 22--32 DEG C, vacuum degree 120-150Pa, make dry materials to moisture < 6%(w), the time about needs 18 hours, is put into after taking-up In freezing crusher, control temperature of charge is no more than 0 DEG C, after being crushed to 100 mesh of fineness >, respectively obtains sea-buckthorn freeze-dried powder, thorn Pears freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder, are fitted into polybag and seal, and are stored in 0 DEG C or less It is spare in freezer.
Embodiment 2
Carbonize the preparation of egg-shell meal:
Eggshell is cleaned up first, dries moisture, is placed in electric baking case, the heated-air drying 2 hours under the conditions of 200 DEG C, After taking-up is let cool, 20 mesh coarse powder are pulverized into, spraying prepared weight ratio on eggshell coarse powder by weight 1:0.5 is 25% Salt solution (i.e. egg-shell meal: 25% salt solution=1:0.5 ratio) is mixed in sprinkling, places 6 hours after mixing, then Uniformly mixing is primary again, then places 6 hours;Impregnated eggshell powder raw material is put into rotary carbonizing pot, in 420 DEG C of backspins Turn charing 80 minutes, (carbonization process prevents charcoal in being equipped with the scraping blade rotated automatically in charing pot until observing the complete blackening of raw material Raw material glues pot and agglomeration during change);The raw material carbonized is rapidly moved in activation furnace, CO is passed through2Gas is caught up with therein to the greatest extent Air, anoxybiotic activates 80 minutes and (controllable stirring is arranged in activation furnace and stirs blade, makes every in activation raw material at 760 DEG C It is stirred within 10 minutes once, guarantees activation material fully and uniformly);After activation, activated material is taken out, is allowed to cool normal Temperature gradually rinses activated material in rinse bath with clear water, until basic inspection does not measure sodium chloride, has cleaned sodium chloride Afterwards, it is submerged activated material 2.5 hours with the hydrochloric acid of mass ratio 12%, is finished wait impregnate, with 95 DEG C of pure water washing activated materials, until Expect that grain filters 7.0 there is no after white, then with room temperature pure water rinse to pH value, obtains rinse activator material;By rinsed clean Activated material, which is dried at 115 DEG C to water content, is lower than 6%, crosses 60 meshes, and the coarse grain after screening is pulverized again, makes all to pass through 60 meshes are to get charing egg-shell meal product.
Embodiment 3
Carbonize the preparation of egg-shell meal:
Eggshell is cleaned up first, dries moisture, is placed in far-infrared dryer, it is 1 hour dry under the conditions of 300 DEG C, it takes After letting cool out, 30 mesh coarse powder are pulverized into, spray the food that prepared weight ratio is 25% on eggshell coarse powder by weight 1:0.5 Salting liquid (i.e. egg-shell meal: 25% salt solution=1:0.5 ratio) is mixed in sprinkling, places 7 hours after mixing, then again Uniformly mixing is primary, then places 7 hours;Impregnated eggshell powder raw material is put into rotary carbonizing pot, is rotated at 450 DEG C Charing 70 minutes, (carbonization process prevents from carbonizing in being equipped with the scraping blade rotated automatically in charing pot until observing the complete blackening of raw material Raw material glues pot and agglomeration in the process);The raw material carbonized is rapidly moved in activation furnace, CO is passed through2Gas catches up with sky therein to the greatest extent Gas, anoxybiotic activates 55 minutes and (controllable stirring is arranged in activation furnace and stirs blade, makes in activation raw material every 10 at 800 DEG C Minute is stirred once, guarantees activation material fully and uniformly);After activation, activated material is taken out, is allowed to cool normal Temperature gradually rinses activated material in rinse bath with clear water, until basic inspection does not measure sodium chloride, has cleaned sodium chloride Afterwards, it is submerged activated material 3 hours with the hydrochloric acid of mass ratio 12%, is finished wait impregnate, with 95 DEG C of pure water washing activated materials, until material Grain filters 7.0 there is no after white, then with room temperature pure water rinse to pH value, obtains rinse activator material;By the work of rinsed clean Material, which is dried at 120 DEG C to water content, is lower than 6%, crosses 60 meshes, the coarse grain after screening is pulverized again, made all by 60 Mesh is to get charing egg-shell meal product.
Embodiment 4
A kind of lactobacillus lactis feed addictive is frozen by lactobacillus lactis dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry Dry powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus charing egg-shell meal be uniformly mixed, compound packet Dress is made: the lactobacillus lactis 30000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 5g, Rosa roxburghii freeze-dried powder 5g, acid Freeze-dried powder 3g, blackcurrant freeze-dried powder 3g, cactus freeze-dried powder 150g are starched, remaining is charing egg-shell meal;Specific production method is to claim The difference of Example 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder It mixes, then lactobacillus lactis dry powder is uniformly admixed, then addition surplus carbonizes egg-shell meal as made from embodiment 2 and is sufficiently mixed After uniformly, composite packaging is carried out;Composite packaging is inner bag using polypropylene plastics pocket, and outer bag uses aluminium foil bag, between interior outer bag Add a few packet silica-gel desiccants, to prevent water suction.
Embodiment 5
A kind of lactobacillus lactis feed addictive is frozen by lactobacillus lactis dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry Dry powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus charing egg-shell meal be uniformly mixed, compound packet Dress is made: the lactobacillus lactis 50000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 50g, Wintercherry freeze-dried powder 3g, blackcurrant freeze-dried powder 3g, cactus freeze-dried powder 135g, remaining is charing egg-shell meal;Specific production method is, Weigh embodiment 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-drying respectively Powder mixes, then lactobacillus lactis dry powder is uniformly admixed, and then it is sufficiently mixed to carbonize egg-shell meal as made from embodiment 2 for addition surplus After closing uniformly, composite packaging is carried out;Composite packaging is inner bag using polypropylene plastics pocket, and outer bag uses aluminium foil bag, interior outer bag it Between plus a few packet silica-gel desiccants, to prevent water suction.
Embodiment 6
A kind of lactobacillus lactis feed addictive is frozen by lactobacillus lactis dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry Dry powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus charing egg-shell meal be uniformly mixed, compound packet Dress is made: the lactobacillus lactis 70000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 15g, Rosa roxburghii freeze-dried powder 50g, Wintercherry freeze-dried powder 8g, blackcurrant freeze-dried powder 8g, cactus freeze-dried powder 90g, remaining is charing egg-shell meal;Specific production method is, Weigh embodiment 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-drying respectively Powder mixes, then lactobacillus lactis dry powder is uniformly admixed, and then it is sufficiently mixed to carbonize egg-shell meal as made from embodiment 2 for addition surplus After closing uniformly, composite packaging is carried out;Composite packaging is inner bag using polypropylene plastics pocket, and outer bag uses aluminium foil bag, interior outer bag it Between plus a few packet silica-gel desiccants, to prevent water suction.
Embodiment 7
A kind of lactobacillus lactis feed addictive is frozen by lactobacillus lactis dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry Dry powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus charing egg-shell meal be uniformly mixed, compound packet Dress is made: the lactobacillus lactis 90000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 25g, Rosa roxburghii freeze-dried powder 25g, Wintercherry freeze-dried powder 5g, blackcurrant freeze-dried powder 5g, cactus freeze-dried powder 85g, remaining is charing egg-shell meal;Specific production method is, Weigh embodiment 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-drying respectively Powder mixes, then lactobacillus lactis dry powder is uniformly admixed, and then it is sufficiently mixed to carbonize egg-shell meal as made from embodiment 2 for addition surplus After closing uniformly, composite packaging is carried out;Composite packaging is inner bag using polypropylene plastics pocket, and outer bag uses aluminium foil bag, interior outer bag it Between plus a few packet silica-gel desiccants, to prevent water suction.
Embodiment 8
A kind of lactobacillus lactis feed addictive is frozen by lactobacillus lactis dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry Dry powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus charing egg-shell meal be uniformly mixed, compound packet Dress is made: the lactobacillus lactis 150000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 15g, Rosa roxburghii freeze-dried powder 30g, Wintercherry freeze-dried powder 15g, blackcurrant freeze-dried powder 15g, cactus freeze-dried powder 75g, remaining is charing egg-shell meal;Specific production method It is to weigh embodiment 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus respectively Freeze-dried powder mixes, then lactobacillus lactis dry powder is uniformly admixed, and then addition surplus carbonizes egg-shell meal as made from embodiment 2 and fills Divide after mixing, carries out composite packaging;Composite packaging is inner bag using polypropylene plastics pocket, and outer bag uses aluminium foil bag, inside and outside Add a few packet silica-gel desiccants between bag, to prevent water suction.
Embodiment 9
A kind of lactobacillus lactis feed addictive is frozen by lactobacillus lactis dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry Dry powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus charing egg-shell meal be uniformly mixed, compound packet Dress is made: the lactobacillus lactis 130000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 25g, Rosa roxburghii freeze-dried powder 25g, Wintercherry freeze-dried powder 20g, blackcurrant freeze-dried powder 20g, cactus freeze-dried powder 90g, remaining is charing egg-shell meal;Specific production method It is to weigh embodiment 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus respectively Freeze-dried powder mixes, then lactobacillus lactis dry powder is uniformly admixed, and then addition surplus carbonizes egg-shell meal as made from embodiment 3 and fills Divide after mixing, carries out composite packaging;Composite packaging is inner bag using polypropylene plastics pocket, and outer bag uses aluminium foil bag, inside and outside Add a few packet silica-gel desiccants between bag, to prevent water suction.
Embodiment 10
A kind of lactobacillus lactis feed addictive is frozen by lactobacillus lactis dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry Dry powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus charing egg-shell meal be uniformly mixed, compound packet Dress is made: the lactobacillus lactis 100000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 25g, Rosa roxburghii freeze-dried powder 20g, Wintercherry freeze-dried powder 30g, blackcurrant freeze-dried powder 30g, cactus freeze-dried powder 95g, remaining is charing egg-shell meal;Specific production method It is to weigh embodiment 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus respectively Freeze-dried powder mixes, then lactobacillus lactis dry powder is uniformly admixed, and then addition surplus carbonizes egg-shell meal as made from embodiment 3 and fills Divide after mixing, carries out composite packaging;Composite packaging is inner bag using polypropylene plastics pocket, and outer bag uses aluminium foil bag, inside and outside Add a few packet silica-gel desiccants between bag, to prevent water suction.
Embodiment 11
A kind of lactobacillus lactis feed addictive is frozen by lactobacillus lactis dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry Dry powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus charing egg-shell meal be uniformly mixed, compound packet Dress is made: the lactobacillus lactis 110000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 40g, Wintercherry freeze-dried powder 12g, blackcurrant freeze-dried powder 12g, cactus freeze-dried powder 80g, remaining is charing egg-shell meal;Specific production method It is to weigh embodiment 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus respectively Freeze-dried powder mixes, then lactobacillus lactis dry powder is uniformly admixed, and then addition surplus carbonizes egg-shell meal as made from embodiment 3 and fills Divide after mixing, carries out composite packaging;Composite packaging is inner bag using polypropylene plastics pocket, and outer bag uses aluminium foil bag, inside and outside Add a few packet silica-gel desiccants between bag, to prevent water suction.
Embodiment 12
A kind of lactobacillus lactis feed addictive is frozen by lactobacillus lactis dry powder, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry Dry powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus charing egg-shell meal be uniformly mixed, compound packet Dress is made: the lactobacillus lactis 120000 hundred million in every 1000g feed addictive, sea-buckthorn freeze-dried powder 50g, Rosa roxburghii freeze-dried powder 50g, Wintercherry freeze-dried powder 10g, blackcurrant freeze-dried powder 10g, cactus freeze-dried powder 100g, remaining is charing egg-shell meal;Specific production method It is to weigh embodiment 1 sea-buckthorn freeze-dried powder obtained, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus respectively Freeze-dried powder mixes, then lactobacillus lactis dry powder is uniformly admixed, and then addition surplus carbonizes egg-shell meal as made from embodiment 3 and fills Divide after mixing, carries out composite packaging;Composite packaging is inner bag using polypropylene plastics pocket, and outer bag uses aluminium foil bag, inside and outside Add a few packet silica-gel desiccants between bag, to prevent water suction.

Claims (9)

1. lactobacillus lactis feed addictive, feature it be first by lactobacillus lactis, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, Wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus charing egg-shell meal be uniformly mixed, Composite packaging is made: lactobacillus lactis 30000 hundred million -150000 hundred million, sea-buckthorn freeze-dried powder 5- in every 1000g feed addictive 50g, Rosa roxburghii freeze-dried powder 5-50g, wintercherry freeze-dried powder 3-30g, blackcurrant freeze-dried powder 3-30g, cactus freeze-dried powder 50-150g, Remaining is charing egg-shell meal;The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-drying Powder is made by following methods, and technical process is that fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus is taken to remove fruit respectively Stalk, the impurity such as rotten fruit, rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step;Rinsing item described above Part is to rinse 15min, and be stirred continuously with the citric acid of 40 DEG C of water addition weight 0.1%, pulls out to be put into pure water and rinses 3 It is secondary;Above-mentioned freezing is will to be cooled to rapidly -45 DEG C or less after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively;It is above-mentioned The process conditions of lyophilization are temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dry until moisture is less than 6%/w; The charing egg-shell meal is made by following methods: (1) dry, dipping first cleans up eggshell, dries moisture, first Row is dried 1-2 hours, is then pulverized into 20-30 mesh coarse powder, is sprayed on eggshell coarse powder by weight 1:0.5 prepared Salt (sodium chloride) solution that weight fraction is 25% is mixed in sprinkling, is placed after mixing 6-7 hours, then uniformly mixed again Unification time, then place 6-7 hours;The purpose is to allow salt effectively to penetrate into eggshell organization internal gap;(2) charing will Impregnated eggshell powder raw material is put into rotary carbonizing pot, is carbonized 70-80 minutes, is seen in 420-450 DEG C of at a temperature of rotation Until examining the complete blackening of raw material;(3) raw material carbonized is rapidly moved in activation furnace by activation, is closed fire door, is passed through titanium dioxide Carbon gas catches up with air therein to the greatest extent, and anoxybiotic activates 55-85 minutes at 720-800 DEG C;(4) it after activated material is taken out in cleaning, allows It is cooled to room temperature, activated material is gradually rinsed in rinse bath with clear water, until not measuring sodium chloride until examining substantially;Cleaning After complete sodium chloride, submerged activated material 2.5-3 hours with the hydrochloric acid that mass ratio is 12%, after impregnating, with 95 DEG C of pure water Activated material is washed, until material grain is there is no after white, then room temperature pure water rinse to neutrality is used, filters, obtain rinse activator material; (5) dry and screening, which dries the activated material of rinsed clean at 115-120 DEG C to water content, is lower than 6%/w, crosses 60 meshes, Coarse grain after screening is pulverized again, make all by 60 meshes to get.
2. lactobacillus lactis feed addictive, feature it be first by lactobacillus lactis, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, Wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus charing egg-shell meal be uniformly mixed, Composite packaging is made: lactobacillus lactis 50000 hundred million -150000 hundred million, sea-buckthorn freeze-dried powder in every 1000g feed addictive 10-50g, Rosa roxburghii freeze-dried powder 10-50g, wintercherry freeze-dried powder 5-30g, blackcurrant freeze-dried powder 5-30g, cactus freeze-dried powder 70- 150g, remaining is charing egg-shell meal;The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, celestial being People slaps freeze-dried powder, is made by following methods, and technical process is to take fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, celestial being respectively The palm removes carpopodium, the impurity such as rotten fruit, and rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step;It is described above Rinsing condition be to be rinsed 15min, and be stirred continuously, pulled out and be put into pure water with the citric acid of 40 DEG C of water addition weight 0.1% Middle rinsing 3 times;Above-mentioned freezing be will be cooled to rapidly after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively -45 DEG C with Under;The process conditions of above-mentioned lyophilization are temperature-22-- 32 DEG C, vacuum degree 120-150Pa, it is dry to moisture less than 6%/ Until w;The charing egg-shell meal is made by following methods: (1) dry, dipping first cleans up eggshell, dries water Point, it is dried 1-2 hours in advance, then pulverizes into 20-30 mesh coarse powder, spray and match on eggshell coarse powder by weight 1:0.5 Salt (sodium chloride) solution that the weight fraction made is 25% mixes in sprinkling, places 6-7 hours after mixing, then again Uniformly mixing is primary, then places 6-7 hours;The purpose is to allow salt effectively to penetrate into eggshell organization internal gap;(2) charcoal Impregnated eggshell powder raw material is put into rotary carbonizing pot by change, carbonizes 70-80 points in 420-450 DEG C of at a temperature of rotation Clock, until observing the complete blackening of raw material;(3) raw material carbonized is rapidly moved in activation furnace by activation, is closed fire door, is passed through Carbon dioxide gas catches up with air therein to the greatest extent, and anoxybiotic activates 55-85 minutes at 720-800 DEG C;(4) activated material is taken out in cleaning Afterwards, it is allowed to cool room temperature, activated material is gradually rinsed in rinse bath with clear water, sodium chloride is not measured until examining substantially and is Only;After having cleaned sodium chloride, submerged activated material 2.5-3 hours with the hydrochloric acid that mass ratio is 12%, after impregnating, with 95 DEG C Pure water washing activated material until material grain is there is no after white, then uses room temperature pure water rinse to neutrality, filters, must rinse work Material;(5) dry and screening, which dries the activated material of rinsed clean at 115-120 DEG C to water content, is lower than 6%/w, crosses 60 Mesh, the coarse grain after screening are pulverized again, make all by 60 meshes to get.
3. lactobacillus lactis feed addictive, feature it be first by lactobacillus lactis, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, Wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus charing egg-shell meal be uniformly mixed, Composite packaging is made: lactobacillus lactis 70000 hundred million -150000 hundred million, sea-buckthorn freeze-dried powder in every 1000g feed addictive 15-50g, Rosa roxburghii freeze-dried powder 15-50g, wintercherry freeze-dried powder 8-30g, blackcurrant freeze-dried powder 8-30g, cactus freeze-dried powder 85- 150g, remaining is charing egg-shell meal powder;The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, Cactus freeze-dried powder, is made by following methods, and technical process is to take fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, celestial being respectively People's palm removes carpopodium, the impurity such as rotten fruit, and rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step;Above-mentioned institute The rinsing condition stated is to rinse 15min, and be stirred continuously with the citric acid of 40 DEG C of water addition weight 0.1%, pull out be put into it is pure It is rinsed 3 times in water;Above-mentioned freezing is will to be cooled to rapidly -45 DEG C after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively Below;The process conditions of above-mentioned lyophilization are temperature-22-- 32 DEG C, and vacuum degree 120-150Pa is dried to moisture and is less than Until 6%/w;The charing egg-shell meal is made by following methods: (1) dry, dipping first cleans up eggshell, dries in the air Solid carbon dioxide point is dried 1-2 hours in advance, then pulverizes into 20-30 mesh coarse powder, spray on eggshell coarse powder by weight 1:0.5 Salt (sodium chloride) solution that prepared weight fraction is 25% is spilt, is mixed in sprinkling, places 6-7 hours after mixing, so Uniformly mixing is primary again afterwards, then places 6-7 hours;The purpose is to allow salt effectively to penetrate into eggshell organization internal gap; (2) impregnated eggshell powder raw material is put into rotary carbonizing pot by charing, carbonizes 70- in 420-450 DEG C of at a temperature of rotation 80 minutes, until observing the complete blackening of raw material;(3) raw material carbonized is rapidly moved in activation furnace by activation, closes fire door, It is passed through carbon dioxide gas, catches up with air therein to the greatest extent, anoxybiotic activates 55-85 minutes at 720-800 DEG C;(4) cleaning, which is taken out, lives After material, it is allowed to cool room temperature, activated material is gradually rinsed in rinse bath with clear water, does not measure sodium chloride until examining substantially Until;After having cleaned sodium chloride, submerged activated material 2.5-3 hours with the hydrochloric acid that mass ratio is 12%, after impregnating, with 95 DEG C pure water washing activated material until material grain is there is no after white, then uses room temperature pure water rinse to neutrality, filters, must rinse Activated material;(5) dry and screening, which dries the activated material of rinsed clean at 115-120 DEG C to water content, is lower than 6%/w, mistake 60 meshes, the coarse grain after screening are pulverized again, make all by 60 meshes to get.
4. lactobacillus lactis feed addictive, feature it be first by lactobacillus lactis, sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, Wintercherry freeze-dried powder, blackcurrant freeze-dried powder, cactus freeze-dried powder be uniformly mixed, then add surplus charing egg-shell meal be uniformly mixed, Composite packaging is made: lactobacillus lactis 90000 hundred million -150000 hundred million, sea-buckthorn freeze-dried powder in every 1000g feed addictive 20-50g, Rosa roxburghii freeze-dried powder 20-50g, wintercherry freeze-dried powder 10-30g, blackcurrant freeze-dried powder 10-30g, cactus freeze-dried powder 100- 150g, remaining is charing egg-shell meal;The sea-buckthorn freeze-dried powder, Rosa roxburghii freeze-dried powder, wintercherry freeze-dried powder, blackcurrant freeze-dried powder, celestial being People slaps freeze-dried powder, is made by following methods, and technical process is to take fresh sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, celestial being respectively The palm removes carpopodium, the impurity such as rotten fruit, and rinsing, freezing, lyophilization, low-temperature grinding are not removed the peel, deseeding step;It is described above Rinsing condition be to be rinsed 15min, and be stirred continuously, pulled out and be put into pure water with the citric acid of 40 DEG C of water addition weight 0.1% Middle rinsing 3 times;Above-mentioned freezing be will be cooled to rapidly after sea-buckthorn, Rosa roxburghii Tratt, wintercherry, blackcurrant, cactus sabot respectively -45 DEG C with Under;The process conditions of above-mentioned lyophilization are temperature-22-- 32 DEG C, vacuum degree 120-150Pa, it is dry to moisture less than 6%/ Until w;The charing egg-shell meal is made by following methods: (1) dry, dipping first cleans up eggshell, dries water Point, it is dried 1-2 hours in advance, then pulverizes into 20-30 mesh coarse powder, spray and match on eggshell coarse powder by weight 1:0.5 Salt (sodium chloride) solution that the weight fraction made is 25% mixes in sprinkling, places 6-7 hours after mixing, then again Uniformly mixing is primary, then places 6-7 hours;The purpose is to allow salt effectively to penetrate into eggshell organization internal gap;(2) charcoal Impregnated eggshell powder raw material is put into rotary carbonizing pot by change, carbonizes 70-80 points in 420-450 DEG C of at a temperature of rotation Clock, until observing the complete blackening of raw material;(3) raw material carbonized is rapidly moved in activation furnace by activation, is closed fire door, is passed through Carbon dioxide gas catches up with air therein to the greatest extent, and anoxybiotic activates 55-85 minutes at 720-800 DEG C;(4) activated material is taken out in cleaning Afterwards, it is allowed to cool room temperature, activated material is gradually rinsed in rinse bath with clear water, sodium chloride is not measured until examining substantially and is Only;After having cleaned sodium chloride, submerged activated material 2.5-3 hours with the hydrochloric acid that mass ratio is 12%, after impregnating, with 95 DEG C Pure water washing activated material until material grain is there is no after white, then uses room temperature pure water rinse to neutrality, filters, must rinse work Material;(5) dry and screening, which dries the activated material of rinsed clean at 115-120 DEG C to water content, is lower than 6%/w, crosses 60 Mesh, the coarse grain after screening are pulverized again, make all by 60 meshes to get.
5. the lactobacillus lactis feed addictive as described in claim 1-4, which is characterized in that the lactobacillus lactis is Commercially available every gram is no less than 250 hundred million thalli dry powder products containing viable bacteria concentration.
6. the lactobacillus lactis feed addictive as described in claim 1-4, which is characterized in that the low-temperature grinding is material Temperature is in 0 DEG C or less and is crushed.
7. the lactobacillus lactis feed addictive as described in claim 1-4, which is characterized in that in the composite packaging uses Outer bag composite packaging, inner bag use polypropylene plastics pocket, and outer packing uses aluminium foil bag, is filled with silica-gel desiccant between interior outer bag Pouch.
8. the lactobacillus lactis feed addictive as described in claim 1-4, which is characterized in that the eggshell be egg shell, Any one or a few mixing eggshell in Ovum Anas domestica putamina, goose egg-shell and quail eggshell.
9. the lactobacillus lactis feed addictive as described in claim 1-4, which is characterized in that the drying uses hot air drying Dry or far-infrared ray drying;The drying condition is 180 DEG C -300 DEG C.
CN201810511982.9A 2018-05-25 2018-05-25 Lactobacillus lactis feed addictive Withdrawn CN109007272A (en)

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Application publication date: 20181218