CN109006481A - A kind of method of cotton embryo sprouting and rooting - Google Patents
A kind of method of cotton embryo sprouting and rooting Download PDFInfo
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- CN109006481A CN109006481A CN201810876053.8A CN201810876053A CN109006481A CN 109006481 A CN109006481 A CN 109006481A CN 201810876053 A CN201810876053 A CN 201810876053A CN 109006481 A CN109006481 A CN 109006481A
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- cotton
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- cotton embryo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The invention discloses a kind of methods of cotton embryo sprouting and rooting, are related to new cotton variety Cultivating techniques field.It include: that selection is bloomed greater than the normal cotton embryo of 25d development；Culture supporter is floated by cotton embryo of porous foam plate；Using transparent germination box as culture carrier, with light water (tap water or distilled water, bottled mineral water etc.) for culture solution；Cotton embryo, which is put in supporter hole, contacts cotton embryo lower part with the water surface；Cystosepiment is put in the transparent germination box equipped with water, will be germinateed to be placed in illumination box after box seals with transparent preservative film and cultivated；For 24 hours, the dark culture time is 0h to light application time, and intensity of illumination 12000lx, cultivation temperature is 30 DEG C.Above method whole operation process and cotton boll, culture substrate and embryo culture are not necessarily to sterilization treatment, easy to operate, are easy to grasp；The above method only needs light water as culture substrate without complicated culture medium and nutrient solution, at low cost, can promote the use of on a large scale.
Description
Technical field
The present invention relates to new cotton variety Cultivating techniques field more particularly to a kind of methods of cotton embryo sprouting and rooting.
Background technique
Crop embryo culture refer to the embryo direct development seedling that plant is induced by artificial in vitro culture and save seed at
Ripe, germination process technology, can effectively shorten generation time.The selection in embryo age and medium component are to children when plant embryo culture
Embryo culture successfully plays key effect.Different plant species suitable embryo culture time and cultural method have differences.Jia Chunlan etc. exists
During the adding generation of Chinese cabbage, the progress of the falling raw embryo in vitro culture in green ripe stage seed is stripped, maturity period 10- can be shortened
20d.35d rataria induces seedling after Yan Huiling etc. pollinates capsicum, can shorten seed maturity 20 to 30d.Mobini and
The rataria in pea Post flowering 13~17d embryo age is placed in HS culture medium and carries out IMMATURE EMBRYOS CULTURE seedling by warkentin etc., can in 1 year
Continuously to breed mostly generation, and it is successfully established 2 pea RILs.Yao etc. research in find, due to environmental condition to pollination of blooming after
The development of embryo has a significant impact, and is suitable for that the embryo age number cultivated is not fixed and invariable.Higher temperature condition, appropriate control water will promote
Into embryonic development, shift to an earlier date the embryo age of suitable in vitro culture.
Currently, cotton belongs to the longer crop of growth cycle, and it is more complicated to belong to tetraploid plant heredity, when cultivating kind
Between it is long, two generations of generation can only be added within cotton 1 year at present, cotton breeding plus generation rely primarily on Planting in the different location and carry out plus generation, and place is
Cotton breeding-Hainan south numerous added-generation 1 year two generation of realization.In terms of In vitro Embryo sprouting and rooting, this grade of Wang Haibo is invented 1 year
Mostly for fast breeding technology, method will induce the direct seedling of rataria in conjunction with shortening developmental process in vitro, and this method can be
Cotton further shortens the cotton breeding period.Ma Zhiying etc. realizes that the cotton of 20~25d of Post flowering can make rataria using culture medium
Direct germination and Plantlet formation shorten seed to mature and Germination And Seedling time.
Conventional method is using culture medium culture, and kinds of culture medium is more, such as: common MS culture medium, the culture medium need 18
Kind reagent and agar, configuration process are comparatively laborious.Routine culture needs gnotobasis and sterile working, as: to culture medium, culture institute
Need triangular flask or culture dish that sterilizing installation is needed to carry out sterilization treatment；Perhaps, cotton boll carries out sterilization treatment, and whole operation mistake
Journey need to operate on superclean bench；Conventional method requires strictly, and misoperation easily causes pollution and culture is caused to fail.It is conventional
Culture medium cotton embryo culture is at high cost, cumbersome, technical requirements are high, can not popularize use on a large scale.
Summary of the invention
In view of this, main purpose is to solve cotton the embodiment of the invention provides a kind of method of cotton embryo sprouting and rooting
Flower embryo cultivates the problem at high cost, cumbersome, technology is complicated and large-scale application is relatively difficult.
In order to achieve the above objectives, invention broadly provides following technical solutions:
On the one hand, the embodiment of the invention provides a kind of method of cotton embryo sprouting and rooting, the method includes the steps:
Cotton embryo: selection is bloomed greater than the normotrophic cotton boll of 25d, and cotton embryo is taken to carry out seedling culture；Wherein, when taking embryo
Cotton boll does not need to carry out sterilization treatment, and operating process carries out under open environment, does not need gnotobasis；
Floating culture supporter: it selects to float on the water surface and the plate-shaped body with multiple through-holes is cultivated as floating
Supporter, the through-hole is used to for the cotton embryo being stuck in the surface of above support so that the cotton embryo is without falling into water；
Culture carrier: select transparent germination box as culture carrier；
Culture substrate: select light water as culture substrate；
The transparent germination box and water are not necessarily to sterilization treatment；Cotton embryo cultivation is put in the culture buoyant support
Through-hole in；The floating culture supporter for being placed with the cotton embryo is placed in the transparent hair equipped with the light water
In bud box；The water surface of the sprouting location contacts of the cotton embryo to the light water；
The transparent germination box for being placed with cotton embryo is sealed using transparent preservative film, forms raising seedling of cotton sealing
Body；
The raising seedling of cotton seal is placed in culture environment and carries out Embryo Culture；
Wherein, culture environment: light application time for 24 hours, dark culture time 0h, 30 DEG C of cultivation temperature, intensity of illumination 12000lx.
Preferably, the detailed process for taking cotton embryo are as follows: first clean cotton boll surface with tap water, then with roll paper or suction
Water paper dries the water on cotton boll surface, is cut cotton boll with blade, and kind shell and cotton embryo are removed, and chooses length greater than 7mm's
Cotton embryo carries out seedling culture.
Preferably, floating culture supporter is cystosepiment, the thickness 5mm of the cystosepiment, the cystosepiment
Thickness design should allow to contact the water surface at the radicle sprouting of cotton embryo.
Preferably, the diameter of the through-hole is 3mm-7mm, the spacing of two neighboring through-hole is 2cm.
Preferably, the quantity for planting the cotton embryo put on the floating culture supporter is 25-30 plants.
Preferably, the light water is tap water, pure water or distilled water.
Compared with prior art, the beneficial effects of the present invention are:
Culture solution of the invention is that light water (such as tap water, pure water or distilled water) is cultivated, and is not necessarily to MS culture medium
Or prepare and contain complicated ingredient nutrient solution, it reduces cost, simplify operation；
Operating environment of the invention be it is open, without operating on superclean bench, reduce environmental requirement, simplify behaviour
Make, reduces cost；
Culture solution that the present invention uses, culture carrier, without professional sterilising apparatus, reduce operation without carrying out sterilization treatment
Difficulty simplifies operating process, reduces toxigenic capacity；
Breeding method of the invention is more obvious for the cotton embryo effect of embryo age 25d or more；
Breeding method of the invention optimizes condition of culture, and illumination is extended for 24 hours, and temperature control is 30 in cultivation temperature
DEG C, seedling fast speed.
Detailed description of the invention
Fig. 1 is the cotton embryo pictorial diagram that 20d embryo age is cultivated on MS culture medium provided by the invention；
Fig. 2 is the cotton embryo seedling pictorial diagram that 20d embryo age is cultivated on MS culture medium provided by the invention；
Fig. 3 is the cotton embryo pictorial diagram that 25d embryo age is cultivated on MS culture medium provided by the invention；
Fig. 4 is the cotton embryo seedling pictorial diagram that 25d embryo age is cultivated on MS culture medium provided by the invention；
Fig. 5 is the cotton embryo culture third day root growth pictorial diagram in common Aquaponic 25d embryo age provided by the invention；
Fig. 6 is the 4th day root growth pictorial diagram of cotton embryo culture in common Aquaponic 25d embryo age provided by the invention；
Fig. 7 is the cotton embryo seedling pictorial diagram in common Aquaponic 25d embryo age provided by the invention.
Specific embodiment
For further illustrate the present invention to reach the technical means and efficacy that predetermined goal of the invention is taken, below with compared with
Good embodiment, to specific embodiment, technical solution, feature and its effect applied according to the present invention, detailed description is as follows.Under
Stating the special characteristic, structure or feature in multiple embodiments in bright can be combined by any suitable form.
Embodiment 1
Cotton Post flowering carries out listed date registration, chooses the normal cotton of development after 25d or 30d, carry out surface clean,
The removing of embryo is carried out, development cotton boll is chosen and is cultivated greater than 7mm embryo；5mm left and right thickness cystosepiment is chosen, length and width can basis
The size of germination box determines；Upper punch is punched on cystosepiment, and aperture diameter size 3-7mm or so is connect with embryo germination portion
Touch the water surface without falling into the water subject to, between hole distance be greater than 2cm；The embryo that will have been removed, is put into the hole accomplished fluently；Choosing
Cotton germination box (germination box is transparent germination box) is taken to pour into distilled water, water is in germination 2/3 position of box；It will be placed with simultaneously
The cystosepiment of cotton embryo is put into germination box, so that it is suspended in the water surface, while being sealed with transparent preservative film；It both can be with after sealing
It is put into illumination box；Operating environment, condition of culture, cultivation results are shown in that Tables 1 and 2, incubation are as shown in Figure 5-Figure 7.
Embodiment 2
2 difference from Example 1 of the present embodiment is that the light water that the present embodiment 2 is selected is bottled mineral water, culture
Operating environment, condition of culture, cultivation results are shown in Tables 1 and 2.
Embodiment 3
3 difference from Example 1 of the present embodiment is that the light water that the present embodiment 3 is selected is tap water, culture operation
Environment, condition of culture, cultivation results are shown in Tables 1 and 2.
Comparative example 1
As shown in Figs 1-4, this comparative example and embodiment the difference is that, be cotton embryo is placed in MS culture medium into
Row culture, then entire culture system is put into transparent sealing equipment and is cultivated；
The specific cultivating process of the comparative example of cotton embryo: MS culture medium (a great number of elements, microelement, molysite, calcium will be prepared
Salt, organic matter, 3% sucrose, agar 0.7%, specific amount of substance can refer to related data), carry out the triangular flask for being dispensed into 150ml
Interior, additional amount about 50ml is sealed with sealed membrane, carries out sterilization treatment with high-pressure sterilizing pot, is taken out triangular flask after having sterilized cold
But the culture after, for subsequent cotton embryo.Cotton Post flowering carries out listed date registration, chooses development normally after 25d or 30d
Cotton.The cotton boll fetched is cleaned to the spot on removal surface with tap water, and is put on the skin with blotting paper dry.It uses on superclean bench surface
70% alcohol sterilizes, and carries out sterilization processing with ultraviolet lamp.In superclean bench, first hand is carried out with 70% alcohol
Sterilizing, then carries out surface sterilizing for the cotton boll after cleaning with 70% alcohol, with the scalpel after sterilizing, cotton boll is cut,
By the removing of embryo, chooses length and be greater than 7mm embryo, be put into the culture dish for bacterium of having gone out in advance；It will will be above-mentioned in superclean bench
Cotton embryo is put into the triangular flask for being pre-loaded with MS culture medium by embryo with the tweezers to sterilize, and cotton embryo radicle sprouts position insertion
On culture medium, every bottle is put into 8 plants or so.Then ParafilmTM is used.Whole operation is sterile working.Cultivate operating environment, training
The condition of supporting, cultivation results are shown in Tables 1 and 2.
The breeding condition of table 1. embodiment 1-3 and comparative example 1
Seedling number of days unit of the 2. embodiment 1-3 of table from comparative example 1 under different condition of culture: d (day)
The embodiment of the present invention 1-3 use three kinds of training liquid, comparative example 1 use MS culture medium, respectively to 15d, 20d, 25d,
30d embryo age carries out different light irradiation times, treatment of different temperature.
The above experimental data shows:
1. the present invention utilizes different culture medium, different condition of culture are compared；As a result, it has been found that: in 20d, 25d, 30d
In cotton immature embryos culture, in the case where handling 4 condition of culture, the seedling result of embryo is superior to control, at remaining in comparative example, embodiment
The condition of reason.Therefore, the experimental results showed that the optimum culture condition the most of cotton embryo are as follows: temperature: 30 DEG C；Light: for 24 hours；It is dark: 0h；
Illumination: 12000Lx.
2. comparative example 1 and embodiment 1-3 are in the culture of 20d, 25d, 30d cotton, cotton under the conditions of identical culture environment
Embryo seedling time difference is smaller.Its comprehensive operation difficulty and cost, embodiment 3 can be used as culture 25 days or more cotton embryo seedlings
Culture substrate.
3. preferably 25d or more cotton embryo carries out seedling culture according to the seedling time in not homeomorphism age.
In conclusion the method for the present invention is to be suitable for being cultivated greater than the cotton embryo of 25d；Culture substrate be water (tap water,
Mineral water or distilled water)；Condition of culture: temperature: 30 DEG C；Light: secretly for 24 hours: 0h；Illumination: 12000Lx.
The successful place of sprouting and rooting breeding method of the present invention is following two points:
1. the cotyledon in cotton embryo has the function of providing nutriment, nutriment can be provided for seedling, selection is big
In the cotton embryo of 25d, each substance of cotyledon accumulates more, enough embryonic development seedlings, and water can provide enough moisture and carry out
Growth therefore, there is no need to additionally to add nutriment and be cultivated.
2. being because culture solution is water, including that nutriment is fewer, together without carrying out related sterilizing in the present embodiment
When, continual illumination for 24 hours can inhibit the growth of some fungies and bacterium in experiment, and the seedling time is shorter, therefore, pole
It is few that pollution example occurs.
Above-mentioned breeding method of the invention is at low cost, technical operation is simple, is easy to grasp, and the big rule of cotton embryo may be implemented
The optimal case of mould culture is conducive to the development of cotton generation technique more than a year.
Place, those skilled in the art can not select from the prior art to the greatest extent in the embodiment of the present invention.
Disclosed above is only a specific embodiment of the invention, but scope of protection of the present invention is not limited thereto, is appointed
What those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, answer
It is included within the scope of the present invention.Therefore, protection scope of the present invention should be with above-mentioned scope of protection of the claims
It is quasi-.
Claims (6)
1. a kind of method of cotton embryo sprouting and rooting, which is characterized in that the described method comprises the following steps:
Cotton embryo: choosing greater than 25 days normotrophic cotton bolls of blooming, and cotton embryo is taken to carry out seedling culture；Wherein, cotton when taking embryo
Bell does not need to carry out sterilization treatment, and operating process carries out under open environment, does not need gnotobasis；
Floating culture supporter: it selects to float on the water surface and the plate-shaped body with multiple through-holes is as floating culture support
Object, the through-hole is used to for the cotton embryo being stuck in the surface of above support so that the cotton embryo is without falling into water；
Culture carrier: select transparent germination box as culture carrier；
Culture substrate: select light water as culture substrate；
The transparent germination box and water are not necessarily to sterilization treatment；The cotton embryo is planted and is put in the logical of the culture buoyant support
In hole；The floating culture supporter for being placed with the cotton embryo is placed in the transparent germination box equipped with the light water
It is interior；The water surface of the sprouting location contacts of the cotton embryo to the light water；
The transparent germination box for being placed with cotton embryo is sealed using transparent preservative film, forms raising seedling of cotton seal；
The raising seedling of cotton seal is placed in culture environment and carries out Embryo Culture；
Wherein, culture environment: light application time for 24 hours, dark culture time 0h, 30 DEG C of cultivation temperature, intensity of illumination 12000lx.
2. a kind of method of cotton embryo sprouting and rooting as described in claim 1, which is characterized in that described to take the specific of cotton embryo
Process are as follows: first clean cotton boll surface with tap water, then dried the water on cotton boll surface with roll paper or blotting paper, with blade by cotton boll
It cuts, kind shell and cotton embryo is removed, choose cotton embryo of the length greater than 7mm and carry out seedling culture.
3. a kind of method of cotton embryo sprouting and rooting as described in claim 1, which is characterized in that supporter is cultivated in the floating
For cystosepiment, the thickness 5mm of the cystosepiment or so.
4. a kind of method of cotton embryo sprouting and rooting as described in claim 1, which is characterized in that the diameter of the through-hole is
3mm-7mm, the spacing of two neighboring through-hole are 2cm.
5. a kind of method of cotton embryo sprouting and rooting as described in claim 1, which is characterized in that supporter is cultivated in the floating
The upper quantity for planting the cotton embryo put is 25-30 plants.
6. a kind of method of cotton embryo sprouting and rooting as described in claim 1, which is characterized in that the light water is originally
Water, pure water or distilled water.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109874665A (en) * | 2019-04-11 | 2019-06-14 | 新疆农业科学院经济作物研究所 | A kind of selection of the salt tolerant cotton variety based on embryo sprouting and rooting |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992010087A1 (en) * | 1990-12-10 | 1992-06-25 | Rhone Poulenc Agrochimie | Artificial seeds |
| CN101120656A (en) * | 2007-09-21 | 2008-02-13 | 河北农业大学 | Culture medium and culture method for cotton immature embryo in vitro culture and plantlet formation |
| CN101263777A (en) * | 2007-12-19 | 2008-09-17 | 华中农业大学 | Method for using sponge block and solution culture facilities to breed vegetable seedling |
| CN102986417A (en) * | 2012-11-14 | 2013-03-27 | 殷勇 | Method for rapidly cultivating cotton seedlings |
| CN104255404A (en) * | 2014-08-27 | 2015-01-07 | 江苏沿海地区农业科学研究所 | Method for rapid hydroponic identification of cotton germplasm salt tolerance and application |
| CN207321822U (en) * | 2017-10-30 | 2018-05-08 | 成都大学 | A kind of germination device |
-
2018
- 2018-08-03 CN CN201810876053.8A patent/CN109006481A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992010087A1 (en) * | 1990-12-10 | 1992-06-25 | Rhone Poulenc Agrochimie | Artificial seeds |
| CN101120656A (en) * | 2007-09-21 | 2008-02-13 | 河北农业大学 | Culture medium and culture method for cotton immature embryo in vitro culture and plantlet formation |
| CN101263777A (en) * | 2007-12-19 | 2008-09-17 | 华中农业大学 | Method for using sponge block and solution culture facilities to breed vegetable seedling |
| CN102986417A (en) * | 2012-11-14 | 2013-03-27 | 殷勇 | Method for rapidly cultivating cotton seedlings |
| CN104255404A (en) * | 2014-08-27 | 2015-01-07 | 江苏沿海地区农业科学研究所 | Method for rapid hydroponic identification of cotton germplasm salt tolerance and application |
| CN207321822U (en) * | 2017-10-30 | 2018-05-08 | 成都大学 | A kind of germination device |
Non-Patent Citations (1)
| Title |
|---|
| 赵岩等: ""破除棉花种子休眠方法的探讨"", 《赤峰学院学报（自然科学版）》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109874665A (en) * | 2019-04-11 | 2019-06-14 | 新疆农业科学院经济作物研究所 | A kind of selection of the salt tolerant cotton variety based on embryo sprouting and rooting |
| CN109874665B (en) * | 2019-04-11 | 2022-06-14 | 新疆农业科学院经济作物研究所 | Breeding method of salt-tolerant cotton variety based on rapid embryo seedling establishment |
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