CN108991091A - A kind of cold fresh beef composite preservative and application containing chitosan oligosaccharide and tea polyphenols - Google Patents

A kind of cold fresh beef composite preservative and application containing chitosan oligosaccharide and tea polyphenols Download PDF

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Publication number
CN108991091A
CN108991091A CN201810836334.0A CN201810836334A CN108991091A CN 108991091 A CN108991091 A CN 108991091A CN 201810836334 A CN201810836334 A CN 201810836334A CN 108991091 A CN108991091 A CN 108991091A
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CN
China
Prior art keywords
beef
tea polyphenols
chitosan oligosaccharide
cold
fresh
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810836334.0A
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Chinese (zh)
Inventor
刘程惠
陈立国
吴轶南
尤晓宏
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Dalian Minzu University
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Dalian Nationalities University
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Publication date
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Priority to CN201810836334.0A priority Critical patent/CN108991091A/en
Publication of CN108991091A publication Critical patent/CN108991091A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B4/00General methods for preserving meat, sausages, fish or fish products
    • A23B4/14Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
    • A23B4/18Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
    • A23B4/20Organic compounds; Microorganisms; Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The present invention relates to a kind of cold fresh beef composite preservative containing chitosan oligosaccharide and tea polyphenols and applications, belong to antistaling agent technical field.A kind of cold fresh beef composite preservative containing chitosan oligosaccharide and tea polyphenols is made of the raw material of following weight percent, chitosan oligosaccharide 0.04-0.06%, tea polyphenols 0.1-0.5%, adds to 100% with water dissolution.It is lengthened using the beef cold storing and fresh-keeping time of the antistaling agent, and the process for making antistaling agent is simple, raw material is environmentally friendly, is easy the utilization that is absorbed by the body, has the function of health care, meets the environment protection requirement of food fresh keeping.

Description

A kind of cold fresh beef composite preservative and application containing chitosan oligosaccharide and tea polyphenols
Technical field
The invention belongs to antistaling agent technical fields, and in particular to a kind of cold fresh compound guarantor of beef containing chitosan oligosaccharide and tea polyphenols Fresh dose.
Background technique
Cold fresh beef quality delicacy, delicious flavour, full of nutrition, protein, potassium and ferro element rich in, especially Its carnitine and content of sarcosine is even more to be much higher than other meats, and metabolism, the enhancing that can speed up body fat are immune Power and the formation for promoting muscle, therefore cold fresh beef already becomes the consumption mainstream in domestic and international meat market instantly.But it is cold Fresh beef quarantines from raw material, butchers, divides acid discharge, then to will appear micro- life during the entire process of packaging, transport, storage, sale It is phenomena such as object pollution, protein oxidation, Pigment degradation and fat oxidation, short so as to cause its shelf life, substantially reduce cold fresh ox The commodity value of meat.Currently, common preservation method is directly to add chemical preservative into cold fresh beef, but part is chemical Antistaling agent, which has an impact to environment and exists to human body, to be endangered, and is queried by consumer.
Summary of the invention
To overcome security risk existing for chemical preservative, the present invention provide it is a kind of for cold fresh beef containing chitosan oligosaccharide and The composite preservative of tea polyphenols.The antistaling agent is not only environmentally friendly, moreover it is possible to improve the immunity of the human body to a certain extent, beneficial to body Body health.
The present invention is as follows using technical solution: a kind of cold antistaling beef agent containing chitosan oligosaccharide and tea polyphenols, effective component Including chitosan oligosaccharide and tea polyphenols, which is made of the raw material of following weight percent, and chitosan oligosaccharide 0.04-0.06%, tea are more Phenol 0.1-0.5% adds to 100% with water dissolution.Chitosan oligosaccharide concentration is 0.06% in preferred formula, tea polyphenols concentration is 0.5%, 100% is added to water dissolution.
The preparation method of the cold fresh beef composite preservative: chitosan oligosaccharide, tea polyphenols are accurately weighed according to formula rate And tea polyphenols stirring and dissolving is added chitosan oligosaccharide progress by water, the water that the mass fraction that water is first added needed for accounting for is 20-30% Ultrasonic wave is dissolved until solution clear, the addition of remaining water is uniformly mixed up to a kind of containing chitosan oligosaccharide again later and The cold antistaling beef agent solution of tea polyphenols.
Another object of the present invention is that the application of above-mentioned composite preservative is claimed, and chooses aged beef, picks Except being divided into beef clod after fascia;It is 6~10MPa to beef surface spraying composite preservative 10~30 seconds in spray pressure;Spray It is put on screen tray after leaching and dries 3~5min in an aseptic environment, carrying out vacuum degree later is that 0.1MPa is packed;After packaging Cold fresh beef is stored in 0-4 DEG C of environment, and wherein the ratio of beef and antistaling agent is 5:1-10:1.
Chitosan oligosaccharide is as chitosan by low polymer obtained by degradation, it is safe and non-toxic and is unique edible Alkaline oligosaccharides, be adjustable intestinal microecology, improve intestinal tissue form and enhancing immune function, have chitosan it is unexistent The water-soluble and easy advantage absorbed by organism.Chitosan oligosaccharide is mainly to pass through the agglutination for influencing bacterial cell membrane lipid, Cause intracellular matter to leak, is finally reached the purpose for destroying cell wall integrity, makes cell that can not form certain knot of tissue Structure, to slow down bacterial multiplication.
Tea polyphenols have the effects that anticancer, antithrombotic, prevention and treatment cardiovascular and cerebrovascular disease and improve immunity.Tea polyphenols pass through increasing Add cell permeability, in conjunction with inhereditary material, interferes the growth and breeding of microorganism, additionally it is possible to act on the enzyme or function of microorganism Energy albumen, is suppressed its normal growth.
The device have the advantages that are as follows:
(1) chitosan oligosaccharide and tea polyphenols of the present invention are easy to be absorbed by the body as antistaling agent, and have the function of health care;
(2) manufacture craft of the present invention is simple, environmentally friendly, meets the environment protection requirement of food fresh keeping.
(3) the beef color stability fresh-keeping using the present invention, organoleptic quality is good, and beef long fresh-keeping period reaches 15~25 It, total plate count and Volatile Base Nitrogen meet national Specification, keep second level freshness range, can be applied to industrial metaplasia It produces.
Specific embodiment
The invention will be further described with reference to embodiments.
Reference examples:
Aged beef is chosen, the beef clod of about 1000g is divided into after rejecting fascia;It is 8MPa to ox in spray pressure It is sprayed 100g water 30 seconds on meat, gnotobasis on screen tray is put into after spray and dries 5min, (vacuum degree is vacuum-packed later 0.1MPa);Cold fresh beef after packaging is stored in 2 DEG C of environment.
Embodiment 1:
Tea polyphenols 0.1% are weighed by following weight percent, the water that the mass fraction of water is 25% is first added needed for accounting for By tea polyphenols stirring and dissolving, adds chitosan oligosaccharide 0.04% and carry out ultrasonic wave dissolution until solution clear, fill into again later Remaining water is uniformly mixed up to a kind of cold antistaling beef agent solution containing chitosan oligosaccharide and tea polyphenols;Choose aged ox Meat is divided into the beef clod of about 1000g after rejecting fascia;It is that 8MPa sprays 100g composite preservative on beef in spray pressure 30 seconds, guarantees composite preservative in beef clod surface filming but do not form drop and be advisable;Gnotobasis on screen tray is put into after spray 5min is dried, is vacuum-packed later (vacuum degree 0.1MPa);Cold fresh beef after packaging is stored in 2 DEG C of environment.
Embodiment 2:
Tea polyphenols 0.1% are weighed by following weight percent, the water that the mass fraction of water is 25% is first added needed for accounting for By tea polyphenols stirring and dissolving, adds chitosan oligosaccharide 0.06% and carry out ultrasonic wave dissolution until solution clear, fill into again later Remaining water is uniformly mixed up to a kind of cold antistaling beef agent solution containing chitosan oligosaccharide and tea polyphenols;It chooses aged Beef is divided into the beef clod of about 1000g after rejecting fascia;It is that 8MPa sprays 100g composite fresh-keeping on beef in spray pressure Agent 30 seconds, guarantees composite preservative in beef clod surface filming but do not form drop and be advisable;Asepsis ring on screen tray is put into after spray 5min is dried in border, is vacuum-packed later (vacuum degree 0.1MPa);Cold fresh beef after packaging is stored in 2 DEG C of environment.
Embodiment 3:
Tea polyphenols 0.2% are weighed by following weight percent, the water that the mass fraction of water is 25% is first added needed for accounting for By tea polyphenols stirring and dissolving, adds chitosan oligosaccharide 0.06% and carry out ultrasonic wave dissolution until solution clear, fill into again later Remaining water is uniformly mixed up to a kind of cold antistaling beef agent solution containing chitosan oligosaccharide and tea polyphenols;It chooses aged Beef is divided into the beef clod of about 1000g after rejecting fascia;It is that 8MPa sprays 100g composite fresh-keeping on beef in spray pressure Agent 30 seconds, guarantees composite preservative in beef clod surface filming but do not form drop and be advisable;Asepsis ring on screen tray is put into after spray 5min is dried in border, is vacuum-packed later (vacuum degree 0.1MPa);Cold fresh beef after packaging is stored in 2 DEG C of environment.
Embodiment 4:
Tea polyphenols 0.3% are weighed by following weight percent, the water that the mass fraction of water is 25% is first added needed for accounting for By tea polyphenols stirring and dissolving, adds chitosan oligosaccharide 0.06% and carry out ultrasonic wave dissolution until solution clear, fill into again later Remaining water is uniformly mixed up to a kind of cold antistaling beef agent solution containing chitosan oligosaccharide and tea polyphenols;It chooses aged Beef is divided into the beef clod of about 1000g after rejecting fascia;It is that 8MPa sprays 100g composite fresh-keeping on beef in spray pressure Agent 30 seconds, guarantees composite preservative in beef clod surface filming but do not form drop and be advisable;Asepsis ring on screen tray is put into after spray 5min is dried in border, is vacuum-packed later (vacuum degree 0.1MPa);Cold fresh beef after packaging is stored in 2 DEG C of environment.
Embodiment 5:
Tea polyphenols 0.5% are weighed by following weight percent, the water that the mass fraction of water is 25% is first added needed for accounting for By tea polyphenols stirring and dissolving, adds chitosan oligosaccharide 0.06% and carry out ultrasonic wave dissolution until solution clear, fill into again later Remaining water is uniformly mixed up to a kind of cold antistaling beef agent solution containing chitosan oligosaccharide and tea polyphenols;It chooses aged Beef is divided into the beef clod of about 1000g after rejecting fascia;It is that 8MPa sprays 100g composite fresh-keeping on beef in spray pressure Agent 30 seconds, guarantees composite preservative in beef clod surface filming but do not form drop and be advisable;Asepsis ring on screen tray is put into after spray 5min is dried in border, is vacuum-packed later (vacuum degree 0.1MPa);Cold fresh beef after packaging is stored in 2 DEG C of environment.
(1) detection method: pass through the microbiological indicator and Volatile Base Nitrogen (TVB-N) of the cold fresh beef in measurement storage front and back Situation of change judge fresh-keeping effect.
The criterion of cold fresh beef freshness rank is as shown in table 1:
1 freshness rank criterion of table
Freshness rank Total plate count/lg (CFU/g) TVB-N/(mg/100g)
Level-one freshness < 4.0 ≤15
Second level freshness 4.0-6.0 15~20
Rotten meat >6.0 ≥20
(2) test result:
Through detecting, the cold fresh beef of control, cold storing and fresh-keeping 10 days, total plate count 5.45g (CFU/g), coliform 5.33lg (CFU/g), TVB-N value 15.97mg/100g, belong to second level freshness range.
The cold fresh beef of embodiment 1, cold storing and fresh-keeping 15 days, total plate count 4.83lg (CFU/g), coliform 5.02lg (CFU/g), TVB-N value 15.47mg/100g belongs to second level freshness range.
The cold fresh beef of embodiment 2, cold storing and fresh-keeping 20 days, total plate count 5.26lg (CFU/g), coliform 5.23lg (CFU/g), TVB-N value 16.00mg/100g belongs to second level freshness range.
The cold fresh beef of embodiment 3, cold storing and fresh-keeping 20 days, total plate count 5.41lg (CFU/g), coliform 5.33lg (CFU/g), TVB-N value 16.73mg/100g belongs to second level freshness range.
The cold fresh beef of embodiment 4, cold storing and fresh-keeping 25 days, total plate count 5.91lg (CFU/g), coliform 5.50lg (CFU/g), TVB-N value 16.96mg/100g belongs to second level freshness range.
The cold fresh beef of embodiment 5, cold storing and fresh-keeping 25 days, total plate count 5.84lg (CFU/g), coliform 5.46lg (CFU/g), TVB-N value 16.44mg/100g belongs to second level freshness range.

Claims (4)

1. a kind of cold fresh beef composite preservative containing chitosan oligosaccharide and tea polyphenols, characterized in that antistaling agent is by following weight percent The raw material of ratio is made, chitosan oligosaccharide 0.04-0.06%, tea polyphenols 0.1-0.5%, adds to 100% with water dissolution.
2. the mass ratio of application of the antistaling agent as described in claim 1 on beef is fresh-keeping, beef and antistaling agent is 5:1-10:1.
3. application according to claim 2, characterized in that choose aged beef, be divided into beef after rejecting fascia Block;It is 6~10MPa to beef surface spraying composite preservative 10~30 seconds in spray pressure;It is put into after spray on screen tray in nothing 3~5min is dried under collarium border, carrying out vacuum degree later is that 0.1MPa is packed;Cold fresh beef after packaging is stored in 0-4 In DEG C environment.
4. a kind of preparation method of the cold antistaling beef agent containing chitosan oligosaccharide and tea polyphenols, characterized in that the following steps are included:
S1. chitosan oligosaccharide, tea polyphenols and water are weighed according to weight ratio;
Tea polyphenols stirring and dissolving is obtained tea polyphenols solution by the water for being S2. 20-30% with the required mass fraction that water is added;
S3. chitosan oligosaccharide is added in S2 tea polyphenols solution, ultrasonic dissolution obtains the mixed solution of clear;
S4. remaining water is added in S3 mixed solution, is stirred evenly.
CN201810836334.0A 2018-07-26 2018-07-26 A kind of cold fresh beef composite preservative and application containing chitosan oligosaccharide and tea polyphenols Pending CN108991091A (en)

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Cited By (6)

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CN110100877A (en) * 2019-06-17 2019-08-09 滁州学院 A kind of Halogen boils the preservation method of Spiced beef
CN110506784A (en) * 2019-09-03 2019-11-29 中华全国供销合作总社杭州茶叶研究所 The compound cold fresh-keeping agent for meat of catechin
CN111011468A (en) * 2019-12-19 2020-04-17 浙江海洋大学 Preparation and application of agar oligosaccharide-gallic acid covalent complex
CN111789158A (en) * 2020-07-16 2020-10-20 无穷食品有限公司 Poultry meat preservative and preparation method and application thereof
CN111838294A (en) * 2020-07-27 2020-10-30 甘肃农业大学 Method for refreshing mutton containing tea polyphenol
CN115024356A (en) * 2022-05-11 2022-09-09 西南大学 Composite biological preservative special for chilled mutton

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110100877A (en) * 2019-06-17 2019-08-09 滁州学院 A kind of Halogen boils the preservation method of Spiced beef
CN110506784A (en) * 2019-09-03 2019-11-29 中华全国供销合作总社杭州茶叶研究所 The compound cold fresh-keeping agent for meat of catechin
CN111011468A (en) * 2019-12-19 2020-04-17 浙江海洋大学 Preparation and application of agar oligosaccharide-gallic acid covalent complex
CN111789158A (en) * 2020-07-16 2020-10-20 无穷食品有限公司 Poultry meat preservative and preparation method and application thereof
CN111838294A (en) * 2020-07-27 2020-10-30 甘肃农业大学 Method for refreshing mutton containing tea polyphenol
CN115024356A (en) * 2022-05-11 2022-09-09 西南大学 Composite biological preservative special for chilled mutton

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Application publication date: 20181214