CN108982502B - Multilayer signal coplane parallel detection device based on gradient reflection - Google Patents
Multilayer signal coplane parallel detection device based on gradient reflection Download PDFInfo
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- CN108982502B CN108982502B CN201810852792.3A CN201810852792A CN108982502B CN 108982502 B CN108982502 B CN 108982502B CN 201810852792 A CN201810852792 A CN 201810852792A CN 108982502 B CN108982502 B CN 108982502B
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Abstract
The invention belongs to the technical field of optical signal detection, and discloses a multilayer signal coplanar parallel detection device based on gradient reflection, which comprises: an optical signal source for generating a multilayer optical signal having an axial displacement difference; a remote focusing assembly for focusing and imaging the multi-layer optical signal at a remote location; the axial reflection compensation component is positioned at the remote position and used for carrying out axial displacement difference compensation on the multilayer optical signal; the area array detector is used for detecting coplanar optical signals formed by the compensated multilayer optical signals; the optical signal source, the remote focusing assembly and the axial reflection compensation assembly are sequentially arranged in the axial direction of the multilayer optical signal. The invention improves the detection efficiency of the detector when obtaining the images of different axial positions of the sample.
Description
Technical Field
The invention relates to the technical field of optical signal detection, in particular to a multilayer signal coplanar parallel detection device based on gradient reflection.
Background
In the field of biological imaging, shallow surface imaging is often used when imaging large samples. The piezoelectric scanner drives the objective lens to move axially or the three-dimensional translation stage drives the sample to generate mechanical cutting to remove the imaged sample part. In this way, the detector sequentially detects different axial position signals of the sample. However, when further increase of the volume of the imaged sample is required, the acquisition time of the microscopic imaging system will be substantially extended. In addition, when detecting transient changes in the biological signal of a living body, the sequential imaging approach cannot obtain a large volume of axial signal before the signal disappears. Therefore, a detection method capable of rapidly obtaining a biological three-dimensional signal is required.
Disclosure of Invention
The invention aims to overcome the technical defects and provides a multilayer optical signal coplanar parallel detection method and device based on gradient reflection so as to shorten the detection time interval of a detector when obtaining images of different axial positions of a sample.
For ease of description, herein are defined: axial, a direction parallel to the multilayer signal propagation direction; the transverse direction is the direction vertical to the axial direction, namely an imaging plane; remote location, imaging location of multi-layer signals.
In order to achieve the above technical object, a technical solution of the present invention provides a multilayer signal coplanar parallel detection apparatus based on gradient reflection, including:
an optical signal source for generating a multilayer optical signal having an axial displacement difference;
a remote focusing assembly for focusing and imaging the multi-layer optical signal at a remote location;
the axial reflection compensation component is positioned at the remote position and used for carrying out axial displacement difference compensation on the multilayer optical signal; and
the planar array detector is used for detecting coplanar optical signals formed by compensating the multilayer optical signals;
the optical signal source, the remote focusing assembly and the axial reflection compensation assembly are sequentially arranged in the axial direction of the multilayer optical signal.
Compared with the prior art, the invention has the beneficial effects that: the coplanar parallel detection of multilayer signals is realized, and the three-dimensional imaging speed of the system is improved; shortening the detection time interval of the detector when the detector obtains different axial positions of the sample; the method has strong applicability to signals with different shapes and different three-dimensional parameters, and breaks through the limitation of simultaneously detecting three-dimensional signals in the traditional imaging mode.
Drawings
FIG. 1 is a schematic diagram of a projection of a multilayer signal in an XZ plane;
FIG. 2 is a schematic projection of a multi-layer signal onto an XY plane;
FIG. 3 is a schematic projection of a multilayer signal onto the YZ plane;
FIG. 4 is a schematic view of the structure of the detection device;
FIG. 5 is a front view of a gradient compensating mirror;
fig. 6 is a right side view of fig. 5.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
For convenience of explanation, an example of a multilayer signal in which the condition is satisfied is given, and signals to which the present invention is applicable are not limited thereto. As shown in FIGS. 1 to 3, the multi-layer signal is a three-strip signal having both axial and transverse intervals, wherein the Z-axis direction is the axial direction, the XY plane is the imaging plane of the parallel objective lens, and the distances of the signals on the imaging plane are respectively Δ x1And Δ x2At axial intervals of the optical signal of Δ z respectively1And Δ z2。
The invention provides a multilayer signal coplanar parallel detection method based on gradient reflection, which comprises the following steps:
and 3, coplanarity of the compensated multilayer signals and clear imaging on the same detection surface.
Preferably, the axial reflection compensation in step 2 comprises the following steps:
step 2.1, focusing and imaging a multilayer signal sent by a signal source at a remote position;
2.2, measuring the axial and transverse intervals among all the component signals in the multilayer signals at a remote position by using a plane mirror;
and 2.3, selecting a gradient compensation reflector matched with the measurement result of the step 2.2, placing the gradient compensation reflector at a remote position, compensating the multilayer signals after calibration, and reflecting and sending the multilayer signals to a detector.
Preferably, the measurement in step 2.2 comprises the following steps:
step 2.21, axially moving the plane mirror until one layer of signals in the multilayer signals can be clearly imaged, and recording the image and the position of the plane mirror;
step 2.22, repeating step 2.21, so that each layer of signals in the multilayer signals are clearly imaged in sequence, and recording the position of the plane mirror and the image when each layer of signals is clearly imaged, wherein the distance between all the recorded positions is the axial interval between all the component signals in the multilayer signals;
and 2.23, superposing all recorded single-layer clear images to the same image, selecting the central position of each component signal as a measurement position, respectively measuring the transverse interval of adjacent component signals, and dividing by the system amplification rate to obtain the transverse interval between the component signals.
Preferably, the calibration in step 2.3 comprises the following steps:
step 2.31, transversely moving the gradient compensation reflector until one layer of signals in the multilayer signals is positioned at the center of the corresponding compensation plane of the gradient compensation reflector;
step 2.32, repeating step 2.31, so that each layer in the multilayer signals is positioned at the center of the corresponding compensation plane of the gradient compensation reflector;
step 2.33, axially moving the gradient compensation reflector until one layer of signals in the multilayer signals can be clearly imaged;
and 2.34, repeating the step 2.33 until the multilayer signals can be clearly imaged, and finishing calibration.
Preferably, the multilayer signal is an optical signal; the component signals of the multi-layer signal have a spacing in both the axial and transverse directions, so that the signals are projected onto the same detection plane simultaneously without overlap.
Preferably, the gradient compensation mirror comprises a plurality of compensation planes arranged in parallel, the axial physical spacing of adjacent compensation planes is half of the axial spacing of corresponding adjacent optical signals, and the lateral distance between the centers of adjacent compensation planes is equal to the lateral distance of corresponding adjacent signals.
The technical solution of the present invention further provides a multilayer signal coplanar parallel detection apparatus based on gradient reflection, as shown in fig. 4, including: an optical signal source, a remote focusing component, an axial reflection compensation component 6 and an area array detector 8.
An optical signal source for generating a multi-layer optical signal having an axial displacement difference.
A remote focusing assembly for focusing and imaging the multi-layer optical signal at a remote location.
An axial reflection compensation component 6 located at the remote location for axial displacement difference compensation of the multilayer optical signal.
And the area array detector 8 is used for detecting coplanar optical signals formed by the compensated multilayer optical signals.
Wherein the optical signal source, the remote focusing assembly and the axial reflection compensation assembly 6 are sequentially arranged in the axial direction of the multilayer optical signal. The multilayer optical signals sequentially pass through the remote focusing assembly and the axial reflection compensation assembly 6 and then are received and detected by the area array detector 8.
Preferably, there is a lateral separation between the constituent signals of the multi-layered optical signal generated by the optical signal source, such that the signals are projected onto the same detection surface simultaneously without overlap.
Preferably, the remote focusing assembly comprises a first objective lens 1, a first lens 2, a second lens 3 and a second objective lens 5, wherein the multilayer optical signal enters from the first objective lens 1, is refracted by the first lens 2 and the second lens 3 in sequence, and then is imaged at a remote position through the second objective lens 5.
Preferably, the numerical aperture of the second objective lens 5 is not smaller than that of the first objective lens 1.
Preferably, the ratio of the refractive indexes of the medium in which the second objective lens 5 and the first objective lens 1 are located is equal to the ratio of the focal lengths of the second lens 3 and the first lens 2. The aberration on the back pupil plane of the first objective lens 1 due to the axial shift of the signals of different layers before the first objective lens 1 will be cancelled by the second objective lens 5, so that the signals with axial shift can be imaged at a remote location without aberration. Wherein, the first lens 2 and the second lens 3 form a lens pair which can transfer the phase of the back pupil surface of the first objective lens 1 to the back pupil surface position of the second objective lens 5.
Preferably, the axial reflection compensation assembly 6 comprises a gradient compensation mirror comprising a plurality of compensation planes arranged in parallel, each layer of the multilayer optical signal being reflected on a corresponding reflection surface; the axial physical separation of adjacent compensation planes is half of the axial spacing of corresponding adjacent optical signals, and the lateral distance of the centers of adjacent compensation planes is equal to the lateral distance of corresponding adjacent signals.
As shown in fig. 5 and 6, M1、M2And M3Compensation planes of the gradient compensation mirror, respectively, the spacing of the compensation planes in the x-direction of the XY plane being the same as the distance of the multilayer signal in the imaging plane, respectively, Δ x1And Δ x2. The axial spacing of the compensation planes is respectively half of the axial spacing of the corresponding adjacent optical signals, i.e. the axial spacing of the compensation planes is respectively 1/2 Δ z1And 1/2 Δ z2。
Preferably, each compensation plane of the gradient compensation mirror is arranged perpendicular to an axial direction of the multilayer optical signal.
Preferably, the transition surface between the adjacent compensation planes is perpendicular to the compensation planes to reduce the projection area of the transition surface on the XY plane, thereby increasing the effective area of the compensation planes.
Preferably, the optical fiber laser further comprises a signal beam splitter 4, which is located between the second lens 3 and the second objective lens 5 of the remote focusing assembly, and is used for separating the coplanar optical signal formed after the compensation by the axial reflection compensation assembly 6, and transmitting the coplanar optical signal to the same detection surface of the area array detector 8 for signal detection.
Preferably, the device further comprises a barrel mirror 7, which is located between the signal beam splitter 4 and the area array detector 8, and is used for transmitting the coplanar optical signal separated by the signal beam splitter 4 to the area array detector 8.
The above-mentioned multi-layered optical signal is in the form of a strip, which is only a general embodiment of the present invention and is not intended to limit the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. A multilayer signal coplanar parallel detection device based on gradient reflection is characterized by comprising:
an optical signal source for generating a multilayer optical signal having an axial displacement difference; the axial direction is a direction parallel to the propagation direction of the multilayer signals;
a remote focusing assembly for focusing and imaging the multi-layer optical signal at a remote location;
the axial reflection compensation component is positioned at the remote position and used for carrying out axial displacement difference compensation on the multilayer optical signal; and
the planar array detector is used for detecting coplanar optical signals formed by compensating the multilayer optical signals;
the optical signal source, the remote focusing assembly and the axial reflection compensation assembly are sequentially arranged in the axial direction of the multilayer optical signal; transverse intervals exist among the composition signals of the multilayer optical signals generated by the optical signal source, so that the signals are projected to the same detection surface at the same time without overlapping;
the axial reflection compensation component comprises a gradient compensation reflector, the gradient compensation reflector comprises a plurality of compensation planes which are arranged in parallel, and each layer of signal of the multilayer optical signal is reflected on the corresponding compensation plane; the axial physical interval of the adjacent compensation planes is half of the axial distance of the adjacent component signals in the corresponding multilayer optical signals, and the transverse distance of the centers of the adjacent compensation planes is equal to the transverse distance of the corresponding adjacent component signals;
each compensation plane of the gradient compensation reflector is arranged perpendicular to the axial direction of the multilayer optical signal;
the transition surface between the adjacent compensation planes is perpendicular to the compensation planes.
2. The apparatus of claim 1, wherein: the remote focusing assembly comprises a first objective lens, a first lens, a second lens and a second objective lens, wherein the multilayer optical signal enters from the first objective lens, is refracted by the first lens and the second lens in sequence, and then is imaged at a remote position through the second objective lens.
3. The apparatus of claim 2, wherein: the numerical aperture of the second objective lens is not smaller than that of the first objective lens.
4. The apparatus of claim 2, wherein: the ratio of the refractive indexes of the medium where the second objective lens and the first objective lens are located is equal to the ratio of the focal lengths of the second lens and the first lens.
5. The apparatus of claim 1, wherein: the signal beam splitter is positioned between the second lens and the second objective of the remote focusing assembly and used for separating coplanar optical signals formed after the compensation of the axial reflection compensation assembly and transmitting the coplanar optical signals to the same detection surface of the area array detector for clear imaging.
6. The apparatus of claim 5, wherein: the cylindrical mirror is positioned between the signal beam splitter and the area array detector.
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| JP2007213708A (en) * | 2006-02-09 | 2007-08-23 | Pulstec Industrial Co Ltd | Optical pickup device for multilayer optical disk |
| CN101089893A (en) * | 2007-07-19 | 2007-12-19 | 宋兆会 | 3D mould set method based on picture and its used multi-equidistance parallel linear form light emission device |
| CN101819319A (en) * | 2009-12-28 | 2010-09-01 | 中国科学院西安光学精密机械研究所 | Fluorescence microscopy method to generate multi-layer polished sections by utilizing Fresnel biprism and device |
| WO2018052905A1 (en) * | 2016-09-16 | 2018-03-22 | The Trustees Of Columbia University In The City Of New York | Three-dimensional imaging using swept, confocally aligned planar excitation and a customized image splitter |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007054351A1 (en) * | 2005-11-11 | 2007-05-18 | Siemens Aktiengesellschaft | Measuring system for three-dimensional objects |
| JP2007213708A (en) * | 2006-02-09 | 2007-08-23 | Pulstec Industrial Co Ltd | Optical pickup device for multilayer optical disk |
| CN101089893A (en) * | 2007-07-19 | 2007-12-19 | 宋兆会 | 3D mould set method based on picture and its used multi-equidistance parallel linear form light emission device |
| CN101819319A (en) * | 2009-12-28 | 2010-09-01 | 中国科学院西安光学精密机械研究所 | Fluorescence microscopy method to generate multi-layer polished sections by utilizing Fresnel biprism and device |
| WO2018052905A1 (en) * | 2016-09-16 | 2018-03-22 | The Trustees Of Columbia University In The City Of New York | Three-dimensional imaging using swept, confocally aligned planar excitation and a customized image splitter |
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