CN108977478A - A method of utilizing bean dregs hydrolyzate fermented-producing bacteria cellulose - Google Patents
A method of utilizing bean dregs hydrolyzate fermented-producing bacteria cellulose Download PDFInfo
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- CN108977478A CN108977478A CN201810812041.9A CN201810812041A CN108977478A CN 108977478 A CN108977478 A CN 108977478A CN 201810812041 A CN201810812041 A CN 201810812041A CN 108977478 A CN108977478 A CN 108977478A
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- 239000001913 cellulose Substances 0.000 title claims abstract description 63
- 229920002678 cellulose Polymers 0.000 title claims abstract description 63
- 244000046052 Phaseolus vulgaris Species 0.000 title claims abstract description 55
- 235000010627 Phaseolus vulgaris Nutrition 0.000 title claims abstract description 55
- 238000000034 method Methods 0.000 title claims abstract description 24
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000000843 powder Substances 0.000 claims abstract description 15
- 238000010790 dilution Methods 0.000 claims abstract description 11
- 239000012895 dilution Substances 0.000 claims abstract description 11
- 238000004519 manufacturing process Methods 0.000 claims abstract description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 9
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 18
- 239000001963 growth medium Substances 0.000 claims description 9
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 241000589220 Acetobacter Species 0.000 claims description 5
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 claims description 5
- RGHNJXZEOKUKBD-SQOUGZDYSA-N Gluconic acid Natural products OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 claims description 5
- 239000000174 gluconic acid Substances 0.000 claims description 5
- 235000012208 gluconic acid Nutrition 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 239000000835 fiber Substances 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 abstract description 51
- 238000000855 fermentation Methods 0.000 abstract description 29
- 230000004151 fermentation Effects 0.000 abstract description 29
- 238000002360 preparation method Methods 0.000 abstract description 12
- 238000012545 processing Methods 0.000 abstract description 9
- 238000001035 drying Methods 0.000 abstract description 5
- 230000001954 sterilising effect Effects 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 239000000706 filtrate Substances 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 abstract description 3
- 238000004321 preservation Methods 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 238000012805 post-processing Methods 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 abstract description 2
- 230000003068 static effect Effects 0.000 abstract 2
- 239000002253 acid Substances 0.000 abstract 1
- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 239000012153 distilled water Substances 0.000 description 8
- 239000002609 medium Substances 0.000 description 7
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- 244000005700 microbiome Species 0.000 description 5
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 238000005904 alkaline hydrolysis reaction Methods 0.000 description 3
- 239000006227 byproduct Substances 0.000 description 3
- 150000001720 carbohydrates Chemical class 0.000 description 3
- 235000014633 carbohydrates Nutrition 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
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- 238000012360 testing method Methods 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 2
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- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 239000008104 plant cellulose Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000011218 seed culture Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 235000002918 Fraxinus excelsior Nutrition 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000002956 ash Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000009194 climbing Effects 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000002440 industrial waste Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
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- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000012879 subculture medium Substances 0.000 description 1
- 238000000967 suction filtration Methods 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
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- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
Abstract
The invention discloses a kind of method for hydrolysis of bean dregs and utilize the method for bean dregs hydrolyzate fermented-producing bacteria cellulose, this method comprises the following steps: (1) preparation of bean dregs hydrolyzate: the bean dregs dry powder saved after drying is added in dilution heat of sulfuric acid, it is filtered after being hydrolyzed under high temperature, the filtrate of collection is bean dregs hydrolyzate；(2) preparation of fermentation medium: the bean dregs hydrolyzate of preservation is taken out, and the substances such as yeast powder are added, and adjusts its pH to faintly acid or neutrality, spare after sterilizing；(3) static fermentation: the acetic acid bacteria seed liquor prepared is inoculated into the bean dregs hydrolyzate handled well, is carried out static fermentation and is produced bacteria cellulose.Compared with existing bacteria cellulose zymotechnique, the present invention can not only efficiently produce bacteria cellulose, and by greatly reducing production cost using the waste of food processing, new outlet also is had found for the post-processing of bean dregs simultaneously, with the advantages such as high efficiency, low cost, environmental-friendly, have a good application prospect.
Description
Technical field
The invention belongs to microorganism and fermentation engineering field, be related to a kind of culture substrate for synthesizing bacteria cellulose and
Preparation method, and in particular to a kind of effectively hydrolyzing bean dregs and the method using bean dregs hydrolyzate fermented-producing bacteria cellulose.
Background technique
At the end of the 19th century, British scientist Blang has found bacteria cellulose in experiment for the first time.Bacteria cellulose and plant are fine
Dimension element is the same, is all to be got up the macromolecular formed by glucose by β-Isosorbide-5-Nitrae-glucosides key connection, and compared to plant cellulose,
There are many unique properties for bacteria cellulose.Firstly, the purity of bacteria cellulose is very high, it is practically free of hemicellulose, wooden
The impurity such as element and pectin；Secondly, the elasticity modulus of bacteria cellulose is very high, the usually several times of plant cellulose or even ten numbers
Times, high elastic modulus imparts the high characteristic of bacteria cellulose tensile strength；Bacteria cellulose is with extremely strong water imbibition and thoroughly
Water gas permeability can absorb the moisture of 60-700 times of its dry weight；Bacteria cellulose biocompatibility with higher, adaptability and good
Good biodegradability；It, can be according to requiring, to bacterium fibre finally, bacteria cellulose has Modulatory character in the synthesis process
Dimension element carries out property transformation.Now, bacteria cellulose is widely used to food, papermaking, medicine and other fields, achieves very big
Economic benefit.
China is bean product production and consumption big country, inevitably by-product during bean product processing
The substances such as bean dregs, the new fresh bean dreg that China generates every year at present are up to tens million of tons, these new fresh bean dregs or are dropped or feed
Livestock and poultry, such processing mode do not only result in a series of environmental problems, and also result in the huge wasting of resources.According to
It measures, carbohydrate, crude protein, crude fat and content of ashes are respectively 50-60%, 13-20%, 6-12% and 3- in bean dregs
5%, several kinds of mineral elements and vitamin are furthermore also contained in bean dregs, such as Fe, Ca, Zn, Mg, K, P, Mn and vitamin B.Due to it
High nutrition ingredient allows to be used as the culture substrate of microorganism to produce green product.However, in bean dregs, most of eggs
White matter and carbohydrate exist usually in the form of macromolecule polyalcohol, so being difficult to be directly used as the nutrient source of microorganism.
Therefore, microorganism is improved to the utilization rate of these substances, need to carry out a degree of degradation to it.By bean dregs in certain method
Culture substrate after processing as acetic acid bacteria synthesis bacteria cellulose, not only can be improved micro-organisms bacteria cellulose can
Duration, but also environmental pollution relevant to these industrial wastes are handled can be reduced.
Summary of the invention
The technical problem to be solved in the invention is: providing a kind of side that bacteria cellulose is synthesized using bean dregs hydrolyzate
Method reduces production cost under the premise of guaranteeing high yield bacteria cellulose.
In order to solve the above technical problems, The technical solution adopted by the invention is as follows:
A method of using bean dregs hydrolyzate fermented-producing bacteria cellulose, bacteria cellulose production strain inoculated is arrived
In culture medium containing bean dregs hydrolyzate, fermented-producing bacteria cellulose.
Wherein, the bacteria cellulose production bacterial strain is Chinese Xun Shi gluconic acid acetobacter CCTCC NO:M 2016696.
Wherein, the culture medium containing bean dregs hydrolyzate is formulated as follows:
Bean dregs hydrolyzed liquid fraction 25%~75%, 0~10g/L of yeast powder, 0~5g/L of disodium hydrogen phosphate, citric acid 0
~5g/L；PH 4.0~7.0.
Wherein, the bean dregs hydrolyzate is prepared as follows:
Dry okara powder is added in dilution heat of sulfuric acid, 10~120min is hydrolyzed under the conditions of 100~121 DEG C, is filtered, is collected
Filtrate obtains bean dregs hydrolyzate.
Wherein, the condition of fermented-producing bacteria cellulose is as follows: 25~35 DEG C of 3~15d of culture.
Wherein, the mass fraction of sulfuric acid is 2% in the dilution heat of sulfuric acid.
In the present invention, the preprocess method of bean dregs raw material is as follows: wet bean dregs being laid in pallet, are placed in 80 DEG C of baking ovens
Drying to constant weight, and dry bean dregs are then carried out pulverization process using pulverizer, collect 100 mesh part below, sealing is placed on
The dry, place of being protected from light saves.
The activation method of Chinese Xun Shi gluconic acid acetobacter is as follows: the acetic acid bacteria that will be deposited in 4 DEG C of refrigerators with bevel-faced form
Bacterial strain is transferred to secondary culture base (glucose, 20g/L；Yeast powder, 5g/L；Peptone, 5g/L；Disodium hydrogen phosphate, 2.7g/L；
Citric acid, 1.15g/L；Agar powder, 20g/L, pH 5.0) on, 3-5d is cultivated under conditions of 30 DEG C.Wherein, the acetic acid
Bacterium is Chinese Xun Shi gluconic acid acetobacter CCTCC NO:M 2016696, has been preserved in China typical culture collection center, preservation
Address: the Chinese Wuhan Wuhan University, preservation date on November 29th, 2016.
The preparation of Chinese Xun Shi gluconic acid acetobacter seed liquor: two activated single colonies of picking are inoculated into the kind of 50mL
Sub- culture medium (glucose, 20g/L；Yeast powder, 5g/L；Peptone, 5g/L；Disodium hydrogen phosphate, 2.7g/L；Citric acid, 1.15g/
L, pH 5.0 is divided in 250mL triangular flask), it is cultivated under conditions of 30 DEG C, 150r/min for 24 hours up to seed liquor.
The preparation of fermentation medium: hydrolyzate is taken out, and being diluted to concentration of reduced sugar is about 20g/L or so, adjusts its pH
It is spare after high pressure steam sterilization to 6.0 or so.The objects such as suitable yeast powder, phosphate can be added into hydrolyzate according to demand
Matter improves bacteria cellulose output.
Inoculation and fermentation: the seed liquor of preparation is inoculated into fermentation medium, and control inoculum concentration is 10% (v/v), so
After place it in 30 DEG C of stationary culture 6d.
The processing of bacteria cellulose film: the bacteria cellulose film that system surface layer after fermentation is formed takes out, with distillation
Water repeated flushing, then 2h is impregnated under conditions of 80 DEG C with the NaOH solution of 0.1mol/L.After alkaline hydrolysis, with distilled water and
0.5% acetic acid repeated flushing, until cellulose membrane is in neutrality.Cellulose wet-coating after purifying is placed on clean plate,
It is placed in 80 DEG C of baking ovens that drying to constant weight, institute's value subtracts plate and is self-possessed up to the dry weight of cellulose membrane.
The culture medium that fermentation produces bacteria cellulose at present is mostly semisynthetic medium, the groups such as yeast powder therein, peptone
It is point expensive, cause the fermentation costs of bacteria cellulose higher；And carbohydrate rich in bean dregs, protein,
The nutriments such as fat can not only reduce the production cost of cellulose using bean dregs come fermented-producing bacteria cellulose, moreover it is possible to
Solve bean dregs irrational utilization bring environmental problem.Therefore, the present invention, which provides, a kind of synthesizes bacterium using bean dregs hydrolyzate
The method of cellulose reduces production cost under the premise of guaranteeing high yield bacteria cellulose.
The utility model has the advantages that
The present invention can not only efficiently produce bacteria cellulose, and by being substantially reduced using the waste of food processing
Production cost, while new outlet also is had found for the post-processing of bean dregs, it is excellent with high efficiency, low cost, environmental-friendly etc.
Gesture has a good application prospect.
Detailed description of the invention
The fermentation results figure of Fig. 1 embodiment 1.
The fermentation results figure of Fig. 2 embodiment 2.
Specific embodiment
Embodiment 1: bean dregs hydrolyzate is directly used as culture substrate fermentation production bacteria cellulose after adjusting pH.
(1) preparation of bean dregs hydrolyzate: the dilution heat of sulfuric acid that mass fraction is 2% is prepared with distilled water and the concentrated sulfuric acid, is claimed
It takes 10g to do okara powder to be placed in triangular flask, the sulfuric acid solution for measuring 100mL is added in triangular flask, and sealing, which is placed on, goes out
In bacterium pot, 20min is hydrolyzed under the conditions of 121 DEG C.
(2) dilution of hydrolyzate: after pyrohydrolysis, carrying out suction filtration processing for reaction system, until no liquid ooze for
Only, filtrate is collected, and is settled to 100mL.The concentration of reduced sugar that DNS method measures is about 48g/L, and the distillation of 140mL is added
Water makes concentration of reduced sugar be down to about 20g/L into hydrolyzate.
(3) preparation of fermentation medium: after the bean dregs hydrolyzate pH after dilution is adjusted to 6.0, it is sub-packed in 500mL triangle
In bottle, volume 90mL is dispensed, sterilizing 20min is spare at 121 DEG C.
(4) acetic acid bacteria strain activates: the acetic acid bacteria strain being deposited in 4 DEG C of refrigerators with bevel-faced form is transferred to passage training
It supports on base, cultivates 5d under conditions of 30 DEG C.
(5) preparation of seed liquor: two activated single colonies of picking, be inoculated into the seed culture medium of 50mL 30 DEG C,
It is cultivated under conditions of 150r/min for 24 hours up to seed liquor.
(6) inoculation and fermentation: the seed liquor prepared is inoculated into fermentation medium, and control inoculum concentration is 10% (v/
V), 30 DEG C of stationary culture 6d are placed it in after mixing.
(7) processing of bacteria cellulose film: after fermentation, the bacteria cellulose film that fermented and cultured primary surface is formed is taken
Out, with distilled water repeated flushing, then with the NaOH solution of 0.1mol/L 2h is impregnated under conditions of 80 DEG C.After alkaline hydrolysis, use
Distilled water and 0.5% acetic acid repeated flushing, until cellulose membrane is in neutrality.Cellulose wet-coating after purifying is placed in cleaning
On plate, it is placed in 80 DEG C of baking ovens that drying to constant weight, institute's value subtracts plate and is self-possessed up to the dry weight of cellulose membrane.
(8) in fermentation liquid acetic acid concentration measurement: use high performance liquid chromatography, testing conditions are as follows: chromatographic column:
Bio-Rad HPX-87H；Mobile phase: 0.005mol/L H2SO4；Column temperature: 65 DEG C；Flow velocity: 0.6mL/min；Detection wavelength:
217nm。
(9) Catalysis experiments result: after fermentation, obtained experimental result is as shown in Figure 1.
The 6d the result shows that acetic acid bacteria ferments in bean dregs hydrolyzate, consumes the reduced sugar of 7.52g/L, forms
The bacteria cellulose of 2.01g/L and the by-product acetic acid of 1.69g/L, after fermentation, the pH of culture medium is down to 4.69 by 6.02.
Embodiment 2: make culture substrate fermentation after the optimization of bean dregs hydrolyzate and produce bacteria cellulose.
(1) preparation of bean dregs hydrolyzate: the dilution heat of sulfuric acid that mass fraction is 2% is prepared with distilled water and the concentrated sulfuric acid, is claimed
It takes 10g to do okara powder to be placed in triangular flask, the sulfuric acid solution for measuring 100mL is added in triangular flask, and sealing, which is placed on, goes out
In bacterium pot, 20min is hydrolyzed under the conditions of 121 DEG C.
(2) dilution of hydrolyzate: after pyrohydrolysis, reaction system is divided in 50mL centrifuge tube, in 8000r/
It is centrifuged 20min under conditions of min, discards solid content, collects supernatant, and be settled to 100mL.The reduction that DNS method measures
Sugared concentration is about 48g/L, and the distilled water of 140mL is added into hydrolyzate, concentration of reduced sugar is made to be down to about 20g/L.
(3) optimization of hydrolyzate: real by Plackett-Burman experiment, steepest hill climbing experiment and Box-Behnken
Test, determine hydrolyzate be suitble to bacteria cellulose fermentation condition are as follows: add the yeast powder of 4g/L, the disodium hydrogen phosphate of 1.7g/L,
The citric acid of 1.15g/L adjusts its pH to 6.0 in the bean dregs hydrolyzate after dilution.
(4) preparation of fermentation medium: handling hydrolyzate according to the method described above, is sub-packed in 500mL triangular flask, point
Volume 90mL is filled, sterilizing 20min is spare at 121 DEG C.
(5) acetic acid bacteria strain activates: the acetic acid bacteria strain being deposited in 4 DEG C of refrigerators with bevel-faced form is transferred to passage training
It supports on base, cultivates 5d under conditions of 30 DEG C.
(6) preparation of seed liquor: two activated single colonies of picking, be inoculated into the seed culture medium of 50mL 30 DEG C,
It is cultivated under conditions of 150r/min for 24 hours up to seed liquor.
(7) inoculation and fermentation: the seed liquor prepared is inoculated into fermentation medium, and control inoculum concentration is 10% (v/
V), 30 DEG C of stationary culture 6d are placed it in after mixing.
(8) processing of bacteria cellulose film: after fermentation, the bacteria cellulose film that fermented and cultured primary surface is formed is taken
Out, with distilled water repeated flushing, then with the NaOH solution of 0.1mol/L 2h is impregnated under conditions of 80 DEG C.After alkaline hydrolysis, use
Distilled water and 0.5% acetic acid repeated flushing, until cellulose membrane is in neutrality.Cellulose wet-coating after purifying is placed in cleaning
On plate, it is placed in 80 DEG C of baking ovens that drying to constant weight, institute's value subtracts plate and is self-possessed up to the dry weight of cellulose membrane.
(9) in fermentation liquid acetic acid concentration measurement: use high performance liquid chromatography, testing conditions are as follows: chromatographic column:
Bio-Rad HPX-87H；Mobile phase: 0.005mol/L H2SO4；Column temperature: 65 DEG C；Flow velocity: 0.6mL/min；Detection wavelength:
217nm。
(10) Catalysis experiments result: after fermentation, obtained experimental result is as shown in Figure 2.
The 6d the result shows that acetic acid bacteria ferments in the bean dregs hydrolyzate for being added to yeast powder, disodium hydrogen phosphate, citric acid,
The reduced sugar for consuming 9.23g/L forms the bacteria cellulose of 3.22g/L and the by-product acetic acid of 1.53g/L, fermentation ends
Afterwards, the pH of culture medium is down to 4.87 by 6.02.
Claims (6)
1. a kind of method using bean dregs hydrolyzate fermented-producing bacteria cellulose, which is characterized in that produce bacteria cellulose
Strain inoculated is into the culture medium containing bean dregs hydrolyzate, fermented-producing bacteria cellulose.
2. the method according to claim 1 using bean dregs hydrolyzate fermented-producing bacteria cellulose, the bacterial fibers
Element production bacterial strain is Chinese Xun Shi gluconic acid acetobacter CCTCC NO:M 2016696.
3. the method according to claim 1 using bean dregs hydrolyzate fermented-producing bacteria cellulose, which is characterized in that institute
The culture medium containing bean dregs hydrolyzate is stated, is formulated as follows: bean dregs hydrolyzed liquid fraction 25%~75%, yeast powder 0~
10g/L, 0~5g/L of disodium hydrogen phosphate, 0~5g/L of citric acid；PH 4.0~7.0.
4. the method according to claim 1 or 3 using bean dregs hydrolyzate fermented-producing bacteria cellulose, feature exist
In the bean dregs hydrolyzate is prepared as follows:
Dry okara powder is added in dilution heat of sulfuric acid, 10~120min is hydrolyzed under the conditions of 100~121 DEG C, filter is collected in filtering
Liquid obtains bean dregs hydrolyzate.
5. the method according to claim 1 using bean dregs hydrolyzate fermented-producing bacteria cellulose, which is characterized in that hair
Ferment condition is as follows: 25~35 DEG C of 3~15d of culture.
6. the method according to claim 4 using bean dregs hydrolyzate fermented-producing bacteria cellulose, which is characterized in that institute
The mass fraction of sulfuric acid is 2% in the dilution heat of sulfuric acid stated.
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| LU501659B1 (en) | 2022-02-28 | 2023-08-28 | Univerza V Mariboru | A food waste extract for cultivation of microorganisms and production of bacterial cellulose and a process for obtaining said extract |
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| CN106399421A (en) * | 2016-10-17 | 2017-02-15 | 天津工业大学 | Method of utilizing industrial and agricultural waste soybean meal to produce nanoscale bacterial cellulose |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106399421A (en) * | 2016-10-17 | 2017-02-15 | 天津工业大学 | Method of utilizing industrial and agricultural waste soybean meal to produce nanoscale bacterial cellulose |
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| 陈海超: "醋酸菌发酵产细菌纤维素培养基和培养条件的优化", 《食品工业科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| LU501659B1 (en) | 2022-02-28 | 2023-08-28 | Univerza V Mariboru | A food waste extract for cultivation of microorganisms and production of bacterial cellulose and a process for obtaining said extract |
| EP4234709A1 (en) | 2022-02-28 | 2023-08-30 | Univerza v Mariboru | A food waste extract for cultivation of microorganisms and production of bacterial cellulose and a process for obtaining said extract |
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