CN108546660A - Chitin deacetylase superior strain and its application - Google Patents
Chitin deacetylase superior strain and its application Download PDFInfo
- Publication number
- CN108546660A CN108546660A CN201810328527.5A CN201810328527A CN108546660A CN 108546660 A CN108546660 A CN 108546660A CN 201810328527 A CN201810328527 A CN 201810328527A CN 108546660 A CN108546660 A CN 108546660A
- Authority
- CN
- China
- Prior art keywords
- chitin deacetylase
- rhodococcus equi
- chitin
- fermentation
- deacetylase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
- C12Y305/01041—Chitin deacetylase (3.5.1.41)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The invention belongs to biotechnologies, and in particular to chitin deacetylase superior strain and its application.The chitin deacetylase superior strain is specially Rhodococcus equi (Rhodococcus equi) F6, and deposit number is CGMCC No.14861.The speed of bacterial strain production chitin deacetylase is exceedingly fast, and is that report can produce the Rhodococcus equi of chitin deacetylation, and the most fast bacterial strain of all fermentation production chitin deacetylases in the prior art for the first time at present, is easy to large-scale culture.
Description
Technical field：
The invention belongs to biotechnologies, and in particular to chitin deacetylase superior strain and its application.
Background technology：
Chitin also known as chitin, scientific name are (Isosorbide-5-Nitrae) -2- acetylaminohydroxyphenylarsonic acids 2- deoxidations-callose, are nature lifes
A kind of content contained by object is only second to the glycosaminoglycan of cellulose, is primarily present in invertebrate, as shrimp, insect, seaweed,
In fungi and yeast.But chitin is not soluble in water, sour, alkali and organic solvent, so it is without too big commercial value, and its
Product chitosan after deacetylate is then widely used in the industries such as medicine, food, chemical industry, cosmetics.Production shell is poly- at present
The main chemical method to be used of sugar there are problems, for example, the reaction time is long, energy consumption greatly, unstable product quality, especially arrange
Huge environmental pollution can be caused by putting object.
Chitin deacetylase can take off the production deacetylation of the acetyl group on chitin stabilization, molecular vibrational temperature model
Narrow chitosan product is enclosed, a new approach is provided to solve the problems, such as that chemical method production chitosan exists.It is domestic at present
Only have a small amount of document report, microbe-derived chitin deacetylase about the research of chitin deacetylase producing strains outside
Also mainly based on fungi, bacterium is less.
Currently, relating to the use of the report of the natural microbial fermentation method production chitin deacetylase screened both at home and abroad
Relatively fewer, tri- patent relevances of domestic CN102676485A, CN104450832A and CN104498365A are relatively high.Its
Middle CN102676485A is disclosed with short handle pears spore mould fermented and cultured 90-100 hours, obtains chitin deacetylase；
CN104450832A is disclosed using bacillus alcalophilus fermented and cultured 60-72h, obtains chitin deacetylase；；
CN104498365A is disclosed using strains A spergillus versicolor X fermentation 72-100h, and it is de- to obtain chitin
Acetyl group enzyme crude enzyme liquid.
But that there are fermentation times is long for current microbial fermentation production chitin deacetylase, enzyme activity is low etc. a series of asks
Topic, so the enzyme fails to realize industrialization always at present.Therefore, the excellent producing enzyme novel bacterial of screenability is still from natural environment
It is one of the important topic for solving CDA industrial applications, it may have important practical value and research value.
Invention content：
In view of the deficiencies of the prior art, the present invention is intended to provide the Rhodococcus equi of one plant of energy high yield chitin deacetylase
And its fermentation process and application, the strain enzyme-producing speed of high yield chitin deacetylase provided by the invention are exceedingly fast, and are current
Report can produce the Rhodococcus equi of chitin deacetylation for the first time, and chitin deacetylation is produced in all fermentations in the prior art
The most fast bacterial strain of enzyme, is easy to large-scale culture.
First of the present invention is designed to provide the Rhodococcus equi of plant height production chitin deacetylase
(Rhodococcus equi) F6, the bacterial strain are preserved in Chinese microorganism strain preservation conservator on November 6th, 2017
Meeting common micro-organisms center, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postal
100101 are compiled, deposit number is CGMCC No.14861.
The physicochemical property of Rhodococcus equi (Rhodococcus equi) F6 is as follows：Gram-positive bacteria, LB culture mediums
Upper bacterium colony is spherical in shape, moist, and colony colour is soft pink.
Second object of the present invention is that provide the Rhodococcus equi CGMCC No.14861 takes off in production chitin
Application in acetyl group enzyme.
Third object of the present invention is to provide a kind of using Rhodococcus sp CGMCC No.14861 fermenting and producing chitins
The production method of deacetylase, it is specific as follows：
(1) seed culture
Condition of culture：Speed of agitator is 160-200rpm, and temperature is 30-37 DEG C, and ferment 12-24h；
Seed culture medium：Peptone 5-10g/L, yeast extract 3-8g/L, sodium chloride 5-10g/L, surplus are water, pH6.0-
7.0；
(2) fermented and cultured
Fermentation condition：Inoculum concentration 2-10%, speed of agitator 160-200rpm, temperature are 30-40 DEG C, and ferment 6-10h；
Fermentation medium：Yeast extract 5-10g/L, glucose 0.5-2.0g/L, magnesium sulfate 1.0g/L, potassium dihydrogen phosphate
0.3g/L, dipotassium hydrogen phosphate 1.0g/L, sodium chloride 0.5-2.0g/L, surplus are water, pH6.0-7.0；
It ferments after 6-10h, the chitin deacetylase content in zymotic fluid reaches 170-180U/mL.
Advantageous effect：
Rhodococcus equi CGMCC No.14861 provided by the invention can utilize common carbon source and nitrogen source, quickly carry out cell
The accumulation of culture and chitin deacetylase.By condition optimizing, 6-10h enzymatic productions can reach maximum, per mL zymotic fluids
Enzyme activity is 173.6U.Compared with prior art, the Rhodococcus equi CGMCC No.14861 provided using invention are, it can be achieved that rapidly
Chitin deacetylase is accumulated, achievees the effect that high yield, there is extensive prospects for commercial application.
Description of the drawings：
Fig. 1 is the bacterium colony picture and microscope photo of F6；
Wherein, A is bacterium colony figure；B is microscope photo；
Fig. 2 F6 bacterial strain phylogenetic trees；
Fig. 3 fermentation process curves.
Specific implementation mode：
In order to make the object, technical solution and advantage of this patent be more clearly understood, below in conjunction with specific embodiment, to this
Patent is further elaborated.It should be appreciated that specific embodiment described herein is only used to explain this patent, not
For limiting the present invention.
The screening of 1 F6 bacterial strains of embodiment
The soil sample that the ground such as Shandong, Shenyang, Chengdu acquire is made after suspension and is coated on enriched medium (fine powder chitin
2.5g/L, dipotassium hydrogen phosphate 0.7g/L, magnesium sulfate 0.5g/L, sodium chloride 0.1g/L), then the bacterium solution in enriched medium is passed through
It is coated on screening and culturing medium (tobacco brown spot pathogen 2.5g/L, dipotassium hydrogen phosphate 0.7g/L, potassium dihydrogen phosphate after sterile water dilution
0.3g/L, magnesium sulfate 0.1g/L, paranitroacetanilide 2.0g/L, agar 20g/L) 30 DEG C of cultures 3~5 days.By the training of acquisition
Object transferred species is supported on new solid plate, plate streaking is until there is single bacterium colony.After triangular flask enzymatic production detects secondary screening,
One plant of pure culture is finally obtained, number is F6 bacterial strains.
F6 bacterial strains have been subjected to 16SrDNA identifications, have been evolved by being built with the partial sequence obtained from NCBI blast
It sets (shown in Fig. 2), determines that F6 is Rhodococcus equi (Rhodococcus equi) with MP methods structure chadogram.Rhodococcus sp F6 is in 2017
On November 6, in is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number CGMCC
No.14861。
The enzymatic production of 2 Rhodococcus equi CGMCC No.14861 of embodiment
The Rhodococcus sp CGMCC No.14861 that embodiment 1 obtains are subjected to multiple batches of fermented and cultured.
(1) seed culture
Condition of culture：Speed of agitator is 200rpm, and temperature is 37 DEG C, and ferment 12h；
Seed culture medium is：Peptone 10g/L, yeast extract 5g/L, sodium chloride 10g/L, surplus are water, pH6.0-7.0；
(2) fermented and cultured
Fermentation condition：Inoculum concentration 8%, speed of agitator 200rpm, temperature are 37 DEG C；
Fermentation medium：Yeast extract 5g/L, glucose 0.5g/L, magnesium sulfate 1.0g/L, potassium dihydrogen phosphate 0.3g/L, phosphorus
Sour hydrogen dipotassium 1.0g/L, sodium chloride 0.5g/L, surplus are water, pH6.0-7.0；
Ferment 30h, and zymotic fluid is periodically taken to carry out chitin deacetylase Enzyme activity assay.Enzymatic production curve such as Fig. 3 institutes
Show, reaches maximum enzyme activity when fermenting to 8h, be determined to 173.6U/mL.
Enzyme activity assay method is as follows：
Zymotic fluid 12000r/min centrifuges 5min, is passed through after then washing thalline with the phosphate buffer solution of pH=7.0
Sonicator is crushed, and broken condition is：Power 30% opens 3s and stops 5s, time 55min.Then again through 12000r/
Min centrifuges 5min and obtains crude enzyme liquid.0.3mL crude enzyme liquids be added 0.3mL200mg/L paranitroacetanilide solution and
The phosphate buffer solution of 0.9mLpH=7.0 reacts 15min in 45 DEG C, detects 400nm light absorption values, calculated by standard curve
Go out enzyme activity.
The enzymatic production of 3 Rhodococcus equi CGMCC No.14861 of embodiment
The Rhodococcus sp CGMCC No.14861 that embodiment 1 obtains are subjected to multiple batches of fermented and cultured.
(1) seed culture
Condition of culture：Speed of agitator is 160rpm, and temperature is 30 DEG C, and fermentation is for 24 hours；
Seed culture medium is：Peptone 5g/L, yeast extract 8g/L, sodium chloride 5g/L, surplus are water, pH6.0-7.0；
(2) fermented and cultured
Fermentation condition：Inoculum concentration 10%, speed of agitator 160rpm, temperature are 40 DEG C；
Fermentation medium：Yeast extract 10g/L, glucose 2.0g/L, magnesium sulfate 1.0g/L, potassium dihydrogen phosphate 0.3g/L,
Dipotassium hydrogen phosphate 1.0g/L, sodium chloride 2.0g/L, surplus are water, pH6.0-7.0；
When fermentation is to 6h, zymotic fluid enzyme activity reaches 180.5U/mL after measured.
The substrate catalytic test of 4 enzyme of embodiment
The crude enzyme liquid that case study on implementation 2 obtains is separately added into excessive different substrates (substrate see the table below), substrate is with pH
=4.0 0.2M phosphate-citric acid solution is dissolved, the second discharged by high performance liquid chromatography detection deacetylation
Acid content verifies catalytic activity of the enzyme to different substrates, generates acetic acid content as standard using paranitroacetanilide, passes through inspection
It surveys, finds chitosan and chitin monomer of the crude enzyme liquid to tobacco brown spot pathogen, different deacetylations of strain fermentation generation
N-acetylglucosamine has certain deacetylation, has good directive significance for the commercial application of the enzyme,
Testing result is as follows：
Several embodiments of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
Cannot the limitation to the scope of the claims therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art,
Under the premise of not departing from this patent design, the respective embodiments described above can also make several deformations, combination and improve, these all belong to
In the protection domain of this patent.Therefore, the protection domain of this patent should be subject to claim.
Claims (3)
1. a plant height produces the Rhodococcus equi of chitin deacetylase, which is characterized in that the Rhodococcus equi is specially Ma Hongqiu
Bacterium (Rhodococcus equi) F6, deposit number are CGMCC No.14861.
2. the Rhodococcus equi of high yield chitin deacetylase as described in claim 1 is in producing chitin deacetylase
Using.
3. application of the Rhodococcus equi as claimed in claim 2 in producing chitin deacetylase, which is characterized in that fermentation
The method for producing chitin deacetylase is specific as follows：
(1) seed culture
Condition of culture：Speed of agitator is 160-200rpm, and temperature is 30-37 DEG C, and ferment 12-24h；
Seed culture medium：Peptone 5-10g/L, yeast extract 3-8g/L, sodium chloride 5-10g/L, surplus are water, pH6.0-7.0；
(2) fermented and cultured
Fermentation condition：Inoculum concentration 2-10%, speed of agitator 160-200rpm, temperature are 30-40 DEG C, and ferment 6-10h；
Fermentation medium：Yeast extract 5-10g/L, glucose 0.5-2.0g/L, magnesium sulfate 1.0g/L, potassium dihydrogen phosphate 0.3g/
L, dipotassium hydrogen phosphate 1.0g/L, sodium chloride 0.5-2.0g/L, surplus are water, pH6.0-7.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810328527.5A CN108546660B (en) | 2018-04-13 | 2018-04-13 | Chitin deacetylase high-yield strain and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810328527.5A CN108546660B (en) | 2018-04-13 | 2018-04-13 | Chitin deacetylase high-yield strain and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108546660A true CN108546660A (en) | 2018-09-18 |
| CN108546660B CN108546660B (en) | 2021-05-25 |
Family
ID=63514930
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810328527.5A Active CN108546660B (en) | 2018-04-13 | 2018-04-13 | Chitin deacetylase high-yield strain and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108546660B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110628679A (en) * | 2019-10-09 | 2019-12-31 | 江苏海洋大学 | Raoultella ornithinolytica G10, enzyme production method, product and application |
| CN110699344A (en) * | 2019-10-24 | 2020-01-17 | 山东理工大学 | Comprehensive utilization process of citric acid fermentation tailings |
| CN111154747A (en) * | 2020-01-19 | 2020-05-15 | 天津科技大学 | Method for improving chitin deacetylase yield through mixed fermentation |
| CN111172141A (en) * | 2020-01-19 | 2020-05-19 | 天津科技大学 | Chitin deacetylase |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2171047A1 (en) * | 2007-07-23 | 2010-04-07 | Henkel AG & Co. KGaA | Removal of by-products from crosslinkable preparations |
| CN104109636A (en) * | 2014-06-30 | 2014-10-22 | 浙江树人大学 | Aspergillus versicolor SD-3 and its application in preparation of chitin deacetylase |
| CN104498365A (en) * | 2014-11-17 | 2015-04-08 | 华南理工大学 | Bacterial strain capable of producing chitin deacetylase and application of bacterial strain in production of chitin deacetylase through fermentation |
-
2018
- 2018-04-13 CN CN201810328527.5A patent/CN108546660B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2171047A1 (en) * | 2007-07-23 | 2010-04-07 | Henkel AG & Co. KGaA | Removal of by-products from crosslinkable preparations |
| CN104109636A (en) * | 2014-06-30 | 2014-10-22 | 浙江树人大学 | Aspergillus versicolor SD-3 and its application in preparation of chitin deacetylase |
| CN104498365A (en) * | 2014-11-17 | 2015-04-08 | 华南理工大学 | Bacterial strain capable of producing chitin deacetylase and application of bacterial strain in production of chitin deacetylase through fermentation |
Non-Patent Citations (1)
| Title |
|---|
| 活泼: "高活性甲壳素脱乙酰基酶产生菌种筛选", 《浙江农业科学》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110628679A (en) * | 2019-10-09 | 2019-12-31 | 江苏海洋大学 | Raoultella ornithinolytica G10, enzyme production method, product and application |
| CN110628679B (en) * | 2019-10-09 | 2022-05-24 | 江苏海洋大学 | Raoultella ornithinolytica G10, enzyme production method, product and application |
| CN110699344A (en) * | 2019-10-24 | 2020-01-17 | 山东理工大学 | Comprehensive utilization process of citric acid fermentation tailings |
| CN110699344B (en) * | 2019-10-24 | 2022-03-18 | 山东理工大学 | Comprehensive utilization process of citric acid fermentation tailings |
| CN111154747A (en) * | 2020-01-19 | 2020-05-15 | 天津科技大学 | Method for improving chitin deacetylase yield through mixed fermentation |
| CN111172141A (en) * | 2020-01-19 | 2020-05-19 | 天津科技大学 | Chitin deacetylase |
| CN111154747B (en) * | 2020-01-19 | 2022-04-08 | 天津科技大学 | Method for improving chitin deacetylase yield through mixed fermentation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108546660B (en) | 2021-05-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104911125B (en) | A kind of chitosan enzyme-producing bacteria and its application | |
| CN101555461B (en) | Bacterial strain LT3 producing alkalescence cellulase and breeding method and initial optimization of cellulase production conditions thereof | |
| CN104894017B (en) | A kind of bacillus licheniformis for producing feruloyl esterase and its application | |
| CN108546660A (en) | Chitin deacetylase superior strain and its application | |
| CN104195080A (en) | Bacillus sp capable of producing alginate lyase and application thereof | |
| CN102199554B (en) | Saccharomyces cerevisiae strain with multiple-stress resistance, and application thereof in cellulose alcohol fermentation | |
| CN102174433B (en) | Clostridium beijerinckii with high stress resistance and application thereof | |
| CN103451133B (en) | Bacillus circulans and application for same in preparation for ferulic acid decarboxylase | |
| CN104312928B (en) | One plant of cellulase producing strain and its application | |
| CN101671645B (en) | Method for preparing beta-mannase and special strain thereof | |
| CN103571772A (en) | Novel butanol producing strain and method for producing butanol by using same | |
| CN106811438A (en) | A kind of straw degradative acidifying microbial inoculum and preparation method thereof | |
| CN110484471A (en) | The method that one plant height produces the acidproof bacterial strain of bacteria cellulose and its produces bacteria cellulose | |
| CN108753642A (en) | One plant of production algin catenase bacterial strain Yue Shi Flavobacterium | |
| CN108823116A (en) | One plant of Rhodococcus equi mutant strain for producing chitin deacetylase and its application | |
| CN109666605B (en) | Cellulose biotransformation method of artificial flora | |
| CN117229979B (en) | Extended microbubble strain for producing algin lyase and application thereof | |
| CN110684691A (en) | Preparation process of microbial agent based on directional screening of microorganisms | |
| CN104357364A (en) | Streptomycete strain and method for preparing alkali-resistant salt-resistant xylanase by using same | |
| CN110093298A (en) | Ester perfume (or spice) microbacterium MCDA02 and its method for producing chitin deacetylase | |
| CN102690773B (en) | Enterobacteria strain FY-07 and method thereof for producing bacterial cellulose by static liquid submerged fermentation | |
| CN101531979B (en) | Klebsiella pneumoniae and method of preparing hydrogen and 2, 3-butanediol thereby | |
| CN105349461A (en) | Agarase generating vibrio alginolyticus and application thereof | |
| CN107118980A (en) | Solution keratan microbacterium MCDA02 and its enzyme producing method and product from ocean | |
| CN105368715B (en) | A kind of thermophilic loose mould of degraded cellulose and its application in stalk fermentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |