CN1089722A - 相对荧光强度测定方法 - Google Patents

相对荧光强度测定方法 Download PDF

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Publication number
CN1089722A
CN1089722A CN92114572A CN92114572A CN1089722A CN 1089722 A CN1089722 A CN 1089722A CN 92114572 A CN92114572 A CN 92114572A CN 92114572 A CN92114572 A CN 92114572A CN 1089722 A CN1089722 A CN 1089722A
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China
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fluorescence
relative intensity
peak
intensity
assay method
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CN92114572A
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李杰生
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China Chongqing International Economic And Technical Cooperation Corp
CHONGQING BRANCH OF TRAFFIC BANK
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China Chongqing International Economic And Technical Cooperation Corp
CHONGQING BRANCH OF TRAFFIC BANK
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Priority to CN92114572A priority Critical patent/CN1089722A/zh
Publication of CN1089722A publication Critical patent/CN1089722A/zh
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Abstract

本发明涉及对荧光光谱分析方法的一种改进。 以不同峰、谷的荧光强度之差,即相对荧光强度,作为 测定指标,可方便地提高分析、鉴别效果。在纸币鉴 别中,可以使部分漏检的伪币得以检出。

Description

本发明涉及一种荧光光谱分析方法的改进。
一般荧光光谱分析所检测的是特定波长的荧光强度,而实际上,同一对象的不同波长的荧光强度之差,往往能够进一步描述该对象的特征。例如纸币的荧光鉴别,特征峰波长为440nm左右。在紫外光激发下,真币也并非完全没有荧光(见图1),只是较弱而已,有时候还与伪币的荧光强度接近,甚至更强。这样就有可能造成漏检。实际上伪币除440nm左右有一峰之外,在395nm左右尚有一波谷,二者之差较大(见图2);而真币在395nm左右没有这样的波谷。现有技术没有对这样的相对荧光强度加以利用是一个遗漏。此现有技术可参阅《生物化学检验技术》(人民卫生出版社1990年5月第一版)、CN20910060U和SE8903·358-A等。
本发明所提供的新方法,即可避免现有技术的上述遗漏,使荧光分析的鉴别能力进一步提高。
本发明正是利用不同波长的荧光强度之差,尤其是那些具有特征意义的峰峰、谷谷和峰谷之间的荧光强度之差来作为一种参数,以进一步提高荧光分析的能力。
下面结合附图3,并以纸币鉴别为例,介绍本发明的内容。伪币在紫外光激发下产生440nm左右的波峰1和395nm的波谷2,上述荧光光谱分别由具有特定频率响应的探头3和探头4检出,送往差值放大器5,其输出△F即相对荧光强度,经后级处理后即可作为分析鉴别指标。也可用单色器将峰、谷值分别检出经处理后得到△F。
本发明与现有技术相比,可显著提高荧光分析鉴别的能力,而设备费用的增加极少。
用上述双探头分别对准395nm和440nm进行纸币鉴别,可使相当一部份被目前一般荧光纸币鉴别器漏检的伪币方便、准确地检出。

Claims (3)

1、一种荧光光谱测定方法,其特征在于:测定对象是物质的不同波长的荧光强度之差,即相对荧光强度。
2、由权利要求1所述的荧光光谱测定方法,其特征在于:该相对荧光强度是具有特征意义的峰峰、谷谷和峰谷值之差。
3、由权利要求2后所述的荧光光谱测定方法,其特征在于:在纸币鉴别中,以440nm左右的峰值和395nm左右的谷值之差作为相对荧光强度,以此作为鉴别伪币的指标。
CN92114572A 1992-12-21 1992-12-21 相对荧光强度测定方法 Pending CN1089722A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN92114572A CN1089722A (zh) 1992-12-21 1992-12-21 相对荧光强度测定方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN92114572A CN1089722A (zh) 1992-12-21 1992-12-21 相对荧光强度测定方法

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CN1089722A true CN1089722A (zh) 1994-07-20

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CN92114572A Pending CN1089722A (zh) 1992-12-21 1992-12-21 相对荧光强度测定方法

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1096608C (zh) * 1996-12-09 2002-12-18 吉赛克与德弗连特股份有限公司 检测荧光和磷光的设备和方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1096608C (zh) * 1996-12-09 2002-12-18 吉赛克与德弗连特股份有限公司 检测荧光和磷光的设备和方法

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