CN108967368A - A kind of drosophila anesthesia outfit - Google Patents
A kind of drosophila anesthesia outfit Download PDFInfo
- Publication number
- CN108967368A CN108967368A CN201811175690.9A CN201811175690A CN108967368A CN 108967368 A CN108967368 A CN 108967368A CN 201811175690 A CN201811175690 A CN 201811175690A CN 108967368 A CN108967368 A CN 108967368A
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- China
- Prior art keywords
- drosophila
- anesthesia
- refrigerant
- low temperature
- anaesthetic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 241000255581 Drosophila <fruit fly, genus> Species 0.000 title claims abstract description 149
- 206010002091 Anaesthesia Diseases 0.000 title claims abstract description 79
- 230000037005 anaesthesia Effects 0.000 title claims abstract description 79
- 239000003507 refrigerant Substances 0.000 claims abstract description 64
- 230000003444 anaesthetic effect Effects 0.000 claims abstract description 22
- 238000005057 refrigeration Methods 0.000 claims abstract description 10
- 239000000523 sample Substances 0.000 claims abstract description 10
- 239000004744 fabric Substances 0.000 claims abstract description 9
- 230000002528 anti-freeze Effects 0.000 claims abstract description 8
- 230000001105 regulatory effect Effects 0.000 claims abstract 2
- 239000000463 material Substances 0.000 claims description 12
- 229910052751 metal Inorganic materials 0.000 claims description 8
- 239000002184 metal Substances 0.000 claims description 8
- 239000000615 nonconductor Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- 238000004804 winding Methods 0.000 claims description 3
- 238000009413 insulation Methods 0.000 claims description 2
- 238000002474 experimental method Methods 0.000 abstract description 6
- 238000011160 research Methods 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 230000002631 hypothermal effect Effects 0.000 description 8
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 6
- 206010020591 Hypercapnia Diseases 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 238000012216 screening Methods 0.000 description 5
- 235000013399 edible fruits Nutrition 0.000 description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 239000004411 aluminium Substances 0.000 description 3
- 229910052782 aluminium Inorganic materials 0.000 description 3
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 3
- 229910052802 copper Inorganic materials 0.000 description 3
- 239000010949 copper Substances 0.000 description 3
- 229910001220 stainless steel Inorganic materials 0.000 description 3
- 239000010935 stainless steel Substances 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000255588 Tephritidae Species 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 206010000117 Abnormal behaviour Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000010513 Stupor Diseases 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000001949 anaesthesia Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000007877 drug screening Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 229920000620 organic polymer Polymers 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
Abstract
A kind of drosophila anesthesia outfit, belongs to anesthesia outfit technical field.It is made of low temperature chamber, multiple drosophila anaesthetic rooms, refrigerant block cabin, drosophila anesthesia bottle, drosophila anesthesia bottle bracket, drosophila station, refrigeration compressor and temperature control device；Drosophila station is made of the operating table surface and refrigerant room for being covered with antifreeze cloth, and temperature control device is made of temp probe and temperature controller；Drosophila anesthesia bottle is placed in drosophila anaesthetic room by drosophila anesthesia bottle bracket, and multiple drosophila anaesthetic rooms are placed in low temperature chamber separately, and snakelike heat exchange tube is wrapped in the outer surface of drosophila anaesthetic room；The temperature in low temperature chamber is regulated and controled by refrigeration compressor and temperature controller, makes temperature range -15~-5 degree Celsius；For refrigerant block freight space in the lower part of low temperature chamber, inside is placed with refrigerant block.The device has the characteristics that biological safety is high, environmental-friendly, convenient when realizing drosophila anesthesia, supports to provide instrument as the experiment of model organism, scientific research, teaching and production using drosophila.
Description
Technical field
The invention belongs to anesthesia outfit technical fields, and in particular to a kind of drosophila anesthesia outfit.
Background technique
Drosophila is important genetics research model organism.Drosophila as model organism be widely used in tumour, aging and
The screening of human sexual selection related mechanism and drug, has important application value.
When carrying out the studies above and screening using drosophila as model organism, need to anaesthetize drosophila, in anesthesia
Under drosophila is observed, screened and is shifted.
Currently, there are mainly three types of the technological means anaesthetized both at home and abroad to drosophila, etherization, carbon dioxide narcosis with
And hypothermic anesthesia, three kinds of anesthesia are compared below:
1, etherization: after organic solvent etherization drosophila, ether can be more than 450 small in the intracorporal residual of drosophila
When, the behavior of drosophila of this strong influence, the behavior of drosophila to using drosophila as the related disease molecular mechanism of model organism and
Drug screening is of great significance, the residual effect of the ether authenticity of correlative study.
2, carbon dioxide narcosis: carbon dioxide narcosis drosophila is more mature anesthesia, but related experiment equipment
And experimental situation is more demanding, needs to monitor the oxygen concentration of environment at any time during the experiment, biological safety and environmental-friendly
Property it is insufficient, while carbon dioxide narcosis also be easy to cause the abnormal behavior of drosophila, is unfavorable for the medicine using drosophila as model organism
Object screening operation.
3, hypothermic anesthesia: currently, generally acknowledging in the world relative to ether, carbon dioxide narcosis, row of the hypothermic anesthesia to drosophila
It is minimum to influence.For conditions such as optimum temperature and times for exploring drosophila hypothermic anesthesia, We conducted series of studies, applications
Patent of invention " method that a kind of pair of drosophila implements hypothermic anesthesia " (ZL201410267683.7) and utility model patent " one kind
Drosophila anesthesia outfit " (ZL20140320714.6).Drosophila hypothermic anesthesia obtains more close with its safety and environment friendly
Note, University Of Shanxi has similarly carried out the design of drosophila hypothermic anaesthesia device, and has applied for a kind of utility model patent " liquid-cooling type
Drosophila anesthesia outfit " (ZL201421077554.8).
In order to further increase safety, environment friendly and the convenience of drosophila hypothermic anesthesia, this application provides one
Kind drosophila anesthesia outfit, meets using drosophila as the demand of the experiment of research object, teaching, scientific research and production.
Summary of the invention
The object of the present invention is to provide a kind of drosophila anesthesia outfits, now determine that low temperature (- 15~-5 degrees Celsius) temperature in fact
Drosophila in range is controllably anaesthetized, which has high, environmental-friendly, the convenient and fast spy of biological safety when realizing drosophila anesthesia
Point is supported to provide instrument as the experiment of model organism, scientific research, teaching and production using drosophila.
A kind of drosophila anesthesia outfit of the present invention, by low temperature chamber (copper, aluminium or stainless steel material are made), Duo Geguo
Fly anaesthetic room (cylindrical structure made of copper, aluminium or stainless steel material), refrigerant block cabin (copper, aluminium or stainless steel material system
At), drosophila anesthesia bottle, drosophila anesthesia bottle bracket, drosophila station (being made of the operating table surface and refrigerant room that are covered with antifreeze cloth),
Refrigeration compressor and temperature control device (being made of temp probe and temperature controller) form；Drosophila anesthesia bottle passes through drosophila anesthesia bottle
Bracket is placed in drosophila anaesthetic room, and multiple drosophila anaesthetic rooms are placed in low temperature chamber separately, snakelike heat exchange tube winding
In the outer surface of drosophila anaesthetic room；Be provided on low temperature chamber with drosophila made of heat insulation function material anaesthetize chamber cap 7, with
Guarantee the low temperature environment of low temperature chamber；Temp probe in temperature control device is placed on the top on the inside of low temperature chamber, passes through refrigerant compression
Machine and temperature controller regulate and control the temperature in low temperature chamber, make temperature range -15~0 degree Celsius；Refrigerant block freight space in
The lower part of low temperature chamber, inside are placed with refrigerant block, take out refrigerant block when needing out of refrigerant block cabin and are placed on drosophila station
In refrigerant room；Anesthesia to drosophila is realized in drosophila anaesthetic room, completed in the operating table surface for being covered with antifreeze cloth for a long time or
The observation of short time drosophila, screening and transfer.
Above-mentioned drosophila anesthesia outfit, wherein the drosophila anesthesia bottle bracket and utility model patent
(ZL201420320714.6) drosophila anesthesia bottle bracket is consistent, i.e. the size of drosophila anesthesia bottle bracket and drosophila anesthesia bottle size
It matches, is made of or the metal by being coated by hot non-conductor material is constituted the non-conductor material of heat.Drosophila anesthesia
Bottle can be placed in drosophila anesthesia bottle bracket, is fixed and does not shake.Drosophila anaesthetizes chamber size and drosophila anesthesia bottle bracket ruler
Very little to match, drosophila anesthesia bottle is placed on drosophila anesthesia bottle bracket, and drosophila anesthesia bottle bracket is placed in drosophila anaesthetic room, is consolidated
Determine and does not shake.
Refrigerant is enclosed in the enclosure space that refrigeration compressor and snakelike heat exchange tube are formed, the fruit with snakelike heat exchange tube winding
Fly anaesthetizes chamber outer wall and carries out heat exchange, adjusts temperature in low temperature chamber by the temp probe and temperature controller that are built in low temperature chamber
Degree (temperature of set temperature controller, when measuring in low temperature chamber temperature via temp probe and being higher than set temperature, temperature control
Device adjusts refrigeration compressor start-up operation and freezes, and reduces temperature in low temperature chamber；It is measured in low temperature chamber when via temp probe
Temperature when reaching set temperature, temperature controller control refrigeration compressor stops working).
Refrigerant block freight space can be pulled out in low temperature chamber lower part, refrigerant block cabin in the form of extracting.Inside is placed with refrigerant block cabin
Pallet is provided with water-collecting tray and discharge outlet on the pallet of refrigerant block cabin, and refrigerant block is placed on the pallet of refrigerant block cabin.Refrigerant block is by ice
(metal block class storage cold material is exactly metal block itself, and metal block is in low temperature for a long time for bag, ice chest, metal block class storage cold material
In environment, itself can cool down, and if metal block is transferred out from low temperature environment, it can continue to absorb environment for quite a long time
Heat, to play the role of to environment temperature reduction, this certain environment only be the lesser environment in space, such as our fruit
Fly station；Same ice bag, ice chest are also by storing up cold organic polymer and water or glycerol content and antifreeze cloth or plastics
Shell/outer cover is made, and is placed in low temperature environment and freezes before use, is taken out when use from low temperature environment, ice bag or ice chest
It can continue the heat for absorbing environment for quite a long time, to play the role of to environment temperature reduction, this is also to small spatial loop
The common equipment that border cools down) it is made, size and specification do not require, and can be placed in the refrigerant room of drosophila station
?.Refrigerant block is usually placed in refrigerant block cabin and is pre-chilled, and takes out from refrigerant block cabin be placed on drosophila station when needed
Refrigerant room in, cool down to drosophila station, it is ensured that drosophila is under prolonged narcosis, operator can compared with
The observation, screening and transfer to drosophila are completed in for a long time.Drosophila station is made of glass or low-temperature resistant plastic.
The present invention does not limit the model and specification of the compressor of drosophila anesthesia outfit, refrigerant and temperature controller,
All devices for being able to achieve cooling and temperature control, refrigerant can meet functional need of the invention.Refrigerant does not have with drosophila in the present invention
There is direct contact, refrigerant is enclosed in compressor and heat exchange coiled pipe, will not be formed and be revealed to environment, will not be with experimental subjects
Drosophila or experimental implementation person are contacted, are polluted, which further improves the biological safety of drosophila anesthesia outfit and
Environment friendly.
Operator can select to add in the room of refrigerant room in drosophila station cold according to the time demand of concrete operations
Matchmaker's block does not add the progress long-time drosophila operation of refrigerant block or the operation of short time drosophila.
The drosophila anesthesia outfit that the inside of the invention describes is the equipment suitable for 6 sets and drosophila anesthesia bottle, if necessary
Amplification or contracting appropriate can be carried out based on the basic element of character of the present invention by carrying out more perhaps less drosophila anesthesia simultaneously
Subtract.
Detailed description of the invention
Fig. 1: drosophila anesthesia outfit structural schematic diagram of the present invention；
Fig. 2: the fast cabin schematic diagram of internal structure of refrigerant (top view that Fig. 2 is refrigerant block cabin described in Fig. 1) of the present invention；
Fig. 3: drosophila anesthesia bottle carrier structure schematic diagram of the present invention；
Fig. 4: drosophila anesthesia bottle structural schematic diagram of the present invention；
Fig. 5: drosophila station structural schematic diagram of the present invention；
Wherein the names of the parts are as follows: drosophila anaesthetic room 1 (multiple drosophila anaesthetic rooms 1 are placed on jointly in 1 low temperature chamber 2),
(multiple drosophila anaesthetic rooms share a set of for low temperature chamber 2, snakelike heat exchange tube 3, temp probe 4, snakelike heat exchange tube and compressor interface 5
Snakelike heat exchange tube), refrigerant block cabin 6, drosophila anaesthetize chamber cap 7, refrigerant block cabin pallet 8, refrigerant block 9, water-collecting tray 10, discharge outlet 11；
Drosophila anesthesia bottle bracket 12；Drosophila anesthesia bottle 13；The operating table surface 14 for being covered with antifreeze cloth (can be hemispherical dome structure to be also possible to
Square position structure, be conducive to operation), refrigerant room 15, drosophila station 16.
Compressor, refrigerant, temperature controller model are not required in the present invention, can be realized required temperature control of the present invention
The device of system can be used.
Specific embodiment
It elaborates with reference to the accompanying drawings and examples to the present invention:
Embodiment 1:
Granted patent is reached using temperature in refrigeration compressor, cryogenic probe 4 and temperature controller control low temperature chamber 2
(ZL201410267683.7) temperature；
Drosophila enters drosophila anesthesia bottle 13 through drosophila transfer operation, and drosophila anesthesia bottle is placed on drosophila anesthesia bottle holder 12 and adds
On, it opens drosophila and anaesthetizes chamber cap 7, drosophila anesthesia bottle bracket 12 is put into drosophila anaesthetic room 1 together with drosophila anesthesia bottle, covers fruit
Fly anaesthetizes chamber cap 7, carries out drosophila anesthesia according to granted patent (ZL201410267683.7) time requirement；According to drosophila fiber crops
The use of refrigerant block is selected after liquor-saturated the time required to subsequent operation.
If operation needs the time longer, needs to open refrigerant block cabin 7 using refrigerant block, take out refrigerant block 9, remove fruit
Refrigerant block 9 is put into drosophila station refrigerant room 15, then will be covered with anti-by the operating table surface 14 for being covered with antifreeze cloth of fly station 16
The operating table surface 14 for freezing cloth is covered places on cabin 15 in the drosophila station refrigerant that placed refrigerant block, and composition can be for a long time to fruit
The drosophila station 16 that fly is observed, screened and shifted can be transferred to drosophila behaviour through the postanesthetic drosophila of drosophila anaesthetic room 1
Make to carry out subsequent operation in the operating table surface 14 of platform.
If operation needs the time shorter, do not need that 14 Gai Wei of operating table surface of antifreeze cloth will be covered with using refrigerant block
On the drosophila station refrigerant room 15 for placing refrigerant block, the fruit that can be observed with the short time drosophila, screened and be shifted is formed
Fly station 16, after carrying out in the operating table surface 14 that drosophila station can be transferred to through the postanesthetic drosophila of drosophila anaesthetic room 1
Continuous operation.
When drosophila anesthesia outfit works, the condensed water or frost of the generation of in low temperature chamber and refrigerant block surface stop in instrument
It can only drip and be collected in the water-collecting tray 10 of refrigerant block cabin pallet 8 after use, terminate shutdown after a certain period of time in experiment, draw
The water in water-collecting tray 10 is discharged through discharge outlet 11 in refrigerant block cabin pallet 8 out.
Claims (6)
1. a kind of drosophila anesthesia outfit, it is characterised in that: anaesthetized by low temperature chamber, multiple drosophila anaesthetic rooms, refrigerant block cabin, drosophila
Bottle, drosophila anesthesia bottle bracket, drosophila station, refrigeration compressor and temperature control device composition；Drosophila station is by being covered with antifreeze cloth
Operating table surface and refrigerant room composition, temperature control device is made of temp probe and temperature controller；Drosophila anesthesia bottle passes through drosophila
Anesthesia bottle bracket is placed in drosophila anaesthetic room, and multiple drosophila anaesthetic rooms are placed in low temperature chamber separately, snakelike heat exchange
Pipe is wrapped in the outer surface of drosophila anaesthetic room；It is provided on low temperature chamber with drosophila anaesthetic room made of heat insulation function material
Lid, temp probe is placed on the top on the inside of low temperature chamber, by refrigeration compressor and temperature controller to the temperature in low temperature chamber
Regulated and controled, makes temperature range -15~-5 degree Celsius；For refrigerant block freight space in the lower part of low temperature chamber, inside is placed with refrigerant block.
2. a kind of drosophila anesthesia outfit as described in claim 1, it is characterised in that: the size of drosophila anesthesia bottle bracket and drosophila
Anesthesia bottle size matches, and is made of or the metal structure by being coated by hot non-conductor material the non-conductor material of heat
At；Drosophila anesthesia bottle is placed in drosophila anesthesia bottle bracket, is fixed and is not shaken；Drosophila anaesthetizes chamber size and drosophila anesthesia bottle
Bracket size matches, and drosophila anesthesia bottle is placed on drosophila anesthesia bottle bracket, and drosophila anesthesia bottle bracket is placed on drosophila anaesthetic room
It is interior, it is fixed and does not shake.
3. a kind of drosophila anesthesia outfit as described in claim 1, it is characterised in that: refrigerant be enclosed in refrigeration compressor with it is snakelike
In the enclosure space that heat exchanger tube is formed, heat exchange is carried out with the drosophila anesthesia chamber outer wall of snakelike heat exchange tube winding.
4. a kind of drosophila anesthesia outfit as described in claim 1, it is characterised in that: refrigerant block cabin can be drawn in the form of extracting
Out.
5. a kind of drosophila anesthesia outfit as described in claim 1, it is characterised in that: be placed with refrigerant block cabin inside refrigerant block cabin
Pallet is provided with water-collecting tray and discharge outlet on the pallet of refrigerant block cabin, and refrigerant block is placed on the pallet of refrigerant block cabin.
6. a kind of drosophila anesthesia outfit as described in claim 1, it is characterised in that: refrigerant block is by ice bag, ice chest or metal
Storage cold material is made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811175690.9A CN108967368A (en) | 2018-10-10 | 2018-10-10 | A kind of drosophila anesthesia outfit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811175690.9A CN108967368A (en) | 2018-10-10 | 2018-10-10 | A kind of drosophila anesthesia outfit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108967368A true CN108967368A (en) | 2018-12-11 |
Family
ID=64543413
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811175690.9A Pending CN108967368A (en) | 2018-10-10 | 2018-10-10 | A kind of drosophila anesthesia outfit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108967368A (en) |
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|---|---|---|---|---|
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2018
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