CN108967193A - A kind of Potato Shoot-tips removing fast breeding technique method - Google Patents
A kind of Potato Shoot-tips removing fast breeding technique method Download PDFInfo
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- CN108967193A CN108967193A CN201810889805.4A CN201810889805A CN108967193A CN 108967193 A CN108967193 A CN 108967193A CN 201810889805 A CN201810889805 A CN 201810889805A CN 108967193 A CN108967193 A CN 108967193A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Soil Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of Potato Shoot-tips to remove fast breeding technique method, and the seedbed uses field soil, turf, vermiculite and perlite to mix with the mass ratio of 1:1:1:1；The stipes when cutting section propagation is with 1 section, i.e. one section of a leaf, and upper end will put down its blade, the length of lower end 1-2cm, oblique cutting or lies against media surface.The present invention is simplified and optimizes culture medium, so that potato reproduction speed increases, less, seedling percent is high for easy to operate, band virus；And vermiculite, the perlite of setting can reduce the infection probability of seedling.
Description
Technical field
The present invention relates to potato sets to cultivate field, and specifically a kind of Potato Shoot-tips remove fast breeding technique method.
Background technique
Potato is as a kind of important grain, vegetables and raw material of industry dual-purpose crop, unit area short with growth cycle
Yield height, stem tuber rich in nutrition content, it is widely used the features such as, it has also become be only second in the world rice, wheat, corn the 4th
Generalized grain crop.With the adjustment of agricultural structure, the cultivated area of potato constantly expands.With it is agrotechnical not
Disconnected progress, at present in the cultivating process of potato, potato detoxicating technology is also widely used.Potato detoxicating skill
Art is to cut potato young shoot point production cone, carries out tissue cultures, is bred as detoxification test tube plantlet, and then bear virus-free stem tuber.
And stem apex detoxification, tissue cultures, virus-free potato seed breeding are combined, it is capable of forming a complete fast breeding technique.But
Existing tissue culture medium (TCM) price is more high, so that increased costs.
Summary of the invention
The purpose of the present invention is to provide a kind of Potato Shoot-tips to remove fast breeding technique method, to solve above-mentioned background technique
The problem of middle proposition.
To achieve the above object, the invention provides the following technical scheme:
A kind of Potato Shoot-tips removing fast breeding technique method, includes the following steps:
(1) the not damaged five potato haulm blocks damaged by worms are selected, impregnate potato ball 20min with the gibberellin of 0.50-1mg/kg, so
Potato haulm block is placed on the indoor casting bed of temperature afterwards and educates bud；When bud grows to 4-5cm, the upper end stem apex section of clip 1.5-3cm,
It carries out disinfection after peelling off the blade being clear to the tweezers after disinfection.
(2) the above-mentioned stem apex section stripped is bound up with gauze, is then successively rinsed 20 minutes with tap water, with the second of 70-75%
Alcohol sterilizes 10-20s, with 1% hypochlorite disinfectant 5-6min, then with aseptic water washing 3-5 times, and is blotted with the filter paper after sterilizing
The surface moisture of stem apex section.
(3) to above-mentioned steps (2), treated that stem apex section is removed on the table, first peels spire off in anatomical lens,
Until cutting the stem apex for only having a phyllopodium when exposing the growing point of two phyllopodium.
(4) stem apex stripped is placed in culture medium and is cultivated, the culture medium is preferable with MS culture medium, adds
NAA0.01mg/L, CCC50ppm replace sucrose to breed using table sugar, and sugared concentration is advisable with 20g/L, agar concentration summer
For 7.0g/L, winter 6.5g/L；PH to 6.5 is reconciled in no addition mother liquor when reconciling pH value simultaneously, when drug whole
After addition, then PH is adjusted to 5.6-5.8.
(5) when the stem apex in step (4) is sprouted to 4-6cm, to the seedling of stem apex removing culture by cutting section propagation, every plant
Offspring's a part retain, a part carry out viruses indentification, healthy nontoxic seedling by kind sort out number, in case breeding.
(6) it is quickly bred by the way of cutting section propagation.
(7) by expand it is numerous after detoxification test tube plantlet completely transplanted with tap water Chinese and Western and cultivated into seedbed afterwards.
(8) detoxic seedling stem section is sprayed indolebutyric acid 1 time of 50-200ppm again.
(9) in hot-house culture, every 1h-2h sprays water 1 time, and each time is 5-10min, and night does not spray water, and uses 60-
70% sunshade net carries out sunshade.
(10) by stem section culture 6-9d, observation is taken root after new line, removes sunshade net, technology on daytime water spray, and carry out full-time
Illumination continues to cultivate；After stem section is taken root, every 3-4d sprays 1 nutrient solution.
(11) it is long to 8-10cm to stem section to continue culture, and after there is 3-5 piece blade, detoxic seedling is uprooped, and moves
It plants into greenhouse and carries out original silkworm egg production.
As further scheme of the invention: the seedbed uses field soil, turf, vermiculite and perlite with 1:1:1:1
Mass ratio mix.
As further scheme of the invention: illumination is required in 2000lx-3000lx, light application time in the step (6)
For 14h/d-16h/d；The temperature daytime of tissue culture room controls at 20-25 DEG C.Night controls at 15-20 DEG C；Humid control is in 70%-
80%。
As further scheme of the invention: the stipes in the step (6) when cutting section propagation is with 1 section, i.e., one
Ye Yijie, upper end will put down its blade, the length of lower end 1-2cm, oblique cutting or lie against media surface.
Compared with prior art, the present invention is simplified and optimizes culture medium, so that potato reproduction speed increases, operation letter
Less, seedling percent is high for single, band virus；And vermiculite, the perlite of setting can reduce the infection probability of seedling.
Specific embodiment
The technical solution of the patent is explained in further detail With reference to embodiment.
A kind of Potato Shoot-tips removing fast breeding technique method, includes the following steps:
(1) the not damaged five potato haulm blocks damaged by worms are selected, impregnate potato ball 20min with the gibberellin of 0.50-1mg/kg, so
Potato haulm block is placed on the indoor casting bed of temperature afterwards and educates bud；When bud grows to 4-5cm, the upper end stem apex section of clip 1.5-3cm,
It carries out disinfection after peelling off the blade being clear to the tweezers after disinfection.
(2) the above-mentioned stem apex section stripped is bound up with gauze, is then successively rinsed 20 minutes with tap water, with the second of 70-75%
Alcohol sterilizes 10-20s, with 1% hypochlorite disinfectant 5-6min, then with aseptic water washing 3-5 times, and is blotted with the filter paper after sterilizing
The surface moisture of stem apex section.
(3) to above-mentioned steps (2), treated that stem apex section is removed on the table, first peels spire off in anatomical lens,
Until cutting the stem apex for only having a phyllopodium when exposing the growing point of two phyllopodium.
(4) stem apex stripped is placed in culture medium and is cultivated, the culture medium is preferable with MS culture medium, adds
NAA0.01mg/L, CCC50ppm replace sucrose to breed using table sugar, and sugared concentration is advisable with 20g/L, agar concentration summer
For 7.0g/L, winter 6.5g/L；PH to 6.5 is reconciled in no addition mother liquor when reconciling pH value simultaneously, when drug whole
After addition, then PH is adjusted to 5.6-5.8；The addition of the CCC is so that the young plant stipes of potato shortens, growing way is good, seedling
Strong, dark green leaf color, stalk thickening；NAA enables to well developed root system.
(5) when the stem apex in step (4) is sprouted to 4-6cm, to the seedling of stem apex removing culture by cutting section propagation, every plant
Offspring's a part retain, a part carry out viruses indentification, healthy nontoxic seedling by kind sort out number, in case breeding.
(6) it is quickly bred by the way of cutting section propagation, the stipes when cutting section propagation is with 1 section, i.e., one
Ye Yijie, upper end will put down its blade, the length of lower end 1-2cm, oblique cutting or lie against media surface.
Illumination is required in 2000lx-3000lx, light application time 14h/d-16h/d in breeding；The temperature of tissue culture room is white
Its control is at 20-25 DEG C.Night controls at 15-20 DEG C；Humid control is in 70%-80%.
(7) by expand it is numerous after detoxification test tube plantlet completely transplanted with tap water Chinese and Western and cultivated into seedbed afterwards；The seedling
Bed uses field soil, turf, vermiculite and perlite to mix with the mass ratio of 1:1:1:1.
(8) detoxic seedling stem section is sprayed indolebutyric acid 1 time of 50-200ppm again.
(9) in hot-house culture, every 1h-2h sprays water 1 time, and each time is 5-10min, and night does not spray water, and uses 60-
70% sunshade net carries out sunshade.
(10) by stem section culture 6-9d, observation is taken root after new line, removes sunshade net, technology on daytime water spray, and carry out full-time
Illumination continues to cultivate；After stem section is taken root, every 3-4d sprays 1 nutrient solution.
(11) it is long to 8-10cm to stem section to continue culture, and after there is 3-5 piece blade, detoxic seedling is uprooped, and moves
It plants into greenhouse and carries out original silkworm egg production.
The production cost result for producing 100,000 plants of detoxic seedlings is detailed in following table:
The present invention is simplified and optimizes culture medium, easy to operate, band virus is few so that potato reproduction speed increases, seedling at
Motility rate is high；And vermiculite, the perlite of setting can reduce the infection probability of seedling.
The preferred embodiment of the patent is described in detail above, but this patent is not limited to above-mentioned embodiment party
Formula within the knowledge of one of ordinary skill in the art can also be under the premise of not departing from this patent objective
Various changes can be made.
Claims (5)
1. a kind of Potato Shoot-tips remove fast breeding technique method, which comprises the steps of:
(1) the not damaged five potato haulm blocks damaged by worms are selected, impregnate potato ball 20min with the gibberellin of 0.50-1mg/kg, so
Potato haulm block is placed on the indoor casting bed of temperature afterwards and educates bud；When bud grows to 4-5cm, the upper end stem apex section of clip 1.5-3cm,
It carries out disinfection after peelling off the blade being clear to the tweezers after disinfection；
(2) the above-mentioned stem apex section stripped is bound up with gauze, is then successively rinsed 20 minutes with tap water, is disappeared with the ethyl alcohol of 70-75%
Malicious 10-20s with 1% hypochlorite disinfectant 5-6min, then with aseptic water washing 3-5 times, and blots stem apex with the filter paper after disinfection
The surface moisture of section；
(3) to above-mentioned steps (2), treated that stem apex section is removed on the table, first peels spire off in anatomical lens, until
When exposing the growing point of two phyllopodium, the stem apex for only having a phyllopodium is cut；
(4) stem apex stripped is placed in culture medium and is cultivated, the culture medium is preferable with MS culture medium, adds
NAA0.01mg/L, CCC50ppm replace sucrose to breed using table sugar, and sugared concentration is advisable with 20g/L, agar concentration summer
For 7.0g/L, winter 6.5g/L；PH to 6.5 is reconciled in no addition mother liquor when reconciling pH value simultaneously, when drug whole
After addition, then PH is adjusted to 5.6-5.8；
(5) when the stem apex in step (4) is sprouted to 4-6cm, to the seedling of stem apex removing culture by cutting section propagation, after every plant
Generation a part retains, and a part carries out viruses indentification, and healthy nontoxic seedling sorts out number by kind, in case breeding；
(6) it is quickly bred by the way of cutting section propagation；
(7) by expand it is numerous after detoxification test tube plantlet completely transplanted with tap water Chinese and Western and cultivated into seedbed afterwards；
(8) detoxic seedling stem section is sprayed indolebutyric acid 1 time of 50-200ppm again；
(9) in hot-house culture, every 1h-2h sprays water 1 time, and each time is 5-10min, and night does not spray water, and uses 60-70%
Sunshade net carry out sunshade；
(10) by stem section culture 6-9d, observation is taken root after new line, removes sunshade net, technology on daytime water spray, and carry out Full daylight photograph
Continue to cultivate；After stem section is taken root, every 3-4d sprays 1 nutrient solution；
(11) it is long to 8-10cm to stem section to continue culture, and after there is 3-5 piece blade, detoxic seedling is uprooped, and is transplanted to
Original silkworm egg production is carried out in greenhouse.
2. a kind of Potato Shoot-tips according to claim 1 remove fast breeding technique method, which is characterized in that adopt in the seedbed
It is mixed with field soil, turf, vermiculite and perlite with the mass ratio of 1:1:1:1.
3. a kind of Potato Shoot-tips according to claim 1 remove fast breeding technique method, which is characterized in that the step
(6) illumination is required in 2000lx-3000lx, light application time 14h/d-16h/d in；The temperature daytime of tissue culture room controls in 20-
25℃。
4. night controls at 15-20 DEG C；Humid control is in 70%-80%.
5. a kind of Potato Shoot-tips according to claim 1 remove fast breeding technique method, which is characterized in that the step
(6) stipes in when cutting section propagation is with 1 section, i.e. one section of a leaf, upper end will put down its blade, the length of lower end 1-2cm, oblique cutting
Or lie against media surface.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810889805.4A CN108967193A (en) | 2018-08-07 | 2018-08-07 | A kind of Potato Shoot-tips removing fast breeding technique method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810889805.4A CN108967193A (en) | 2018-08-07 | 2018-08-07 | A kind of Potato Shoot-tips removing fast breeding technique method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111448986A (en) * | 2020-04-09 | 2020-07-28 | 宁夏佳立马铃薯产业有限公司 | Production and preparation method of virus-free seed potatoes |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105191793A (en) * | 2015-09-10 | 2015-12-30 | 宁波市农业科学研究院 | Efficient breeding method of purple potato tissue culture seedlings |
| CN107736244A (en) * | 2017-10-22 | 2018-02-27 | 威海市农业科学院 | Potato Shoot-tips peel off detoxifying fast breeding method |
| CN108157180A (en) * | 2018-01-19 | 2018-06-15 | 内蒙古中薯联企业管理有限公司 | A kind of method of the open industrial fast breeding of potato virus-free plantlet |
-
2018
- 2018-08-07 CN CN201810889805.4A patent/CN108967193A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105191793A (en) * | 2015-09-10 | 2015-12-30 | 宁波市农业科学研究院 | Efficient breeding method of purple potato tissue culture seedlings |
| CN107736244A (en) * | 2017-10-22 | 2018-02-27 | 威海市农业科学院 | Potato Shoot-tips peel off detoxifying fast breeding method |
| CN108157180A (en) * | 2018-01-19 | 2018-06-15 | 内蒙古中薯联企业管理有限公司 | A kind of method of the open industrial fast breeding of potato virus-free plantlet |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111448986A (en) * | 2020-04-09 | 2020-07-28 | 宁夏佳立马铃薯产业有限公司 | Production and preparation method of virus-free seed potatoes |
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