CN108949631A - A kind of application of nucleopore membranes in intestinal contents separation - Google Patents
A kind of application of nucleopore membranes in intestinal contents separation Download PDFInfo
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- CN108949631A CN108949631A CN201810851952.2A CN201810851952A CN108949631A CN 108949631 A CN108949631 A CN 108949631A CN 201810851952 A CN201810851952 A CN 201810851952A CN 108949631 A CN108949631 A CN 108949631A
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- 239000012528 membrane Substances 0.000 title claims abstract description 76
- 238000000926 separation method Methods 0.000 title claims abstract description 43
- 210000003736 gastrointestinal content Anatomy 0.000 title claims abstract description 27
- 244000005700 microbiome Species 0.000 claims abstract description 25
- 239000011148 porous material Substances 0.000 claims abstract description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- 241000186000 Bifidobacterium Species 0.000 claims description 2
- 241000588724 Escherichia coli Species 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 241000194033 Enterococcus Species 0.000 claims 1
- 239000012535 impurity Substances 0.000 abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 9
- 241000894006 Bacteria Species 0.000 abstract description 6
- 238000000605 extraction Methods 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 5
- 230000002550 fecal effect Effects 0.000 abstract description 3
- 239000006185 dispersion Substances 0.000 abstract description 2
- 238000002955 isolation Methods 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 abstract description 2
- 238000001914 filtration Methods 0.000 description 9
- 239000010410 layer Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 239000000243 solution Substances 0.000 description 6
- 210000000936 intestine Anatomy 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 230000000968 intestinal effect Effects 0.000 description 3
- 239000002504 physiological saline solution Substances 0.000 description 3
- 239000013049 sediment Substances 0.000 description 3
- 241001478240 Coccus Species 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000010429 evolutionary process Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 241000605008 Spirillum Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000003486 chemical etching Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000010181 polygamy Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 230000005476 size effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- MEYZYGMYMLNUHJ-UHFFFAOYSA-N tunicamycin Natural products CC(C)CCCCCCCCCC=CC(=O)NC1C(O)C(O)C(CC(O)C2OC(C(O)C2O)N3C=CC(=O)NC3=O)OC1OC4OC(CO)C(O)C(O)C4NC(=O)C MEYZYGMYMLNUHJ-UHFFFAOYSA-N 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
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- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Medicinal Preparation (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of application of nucleopore membranes in intestinal contents separation, in fecal water dispersion liquid after removal of impurities, using nucleopore membrane technology separation and Extraction enteric microorganism, substitute traditional centrifugal isolation technics, the bacterium that can effectively shut off in entire claimed range first, extraction process is standardized, it is more accurate controllable, it is reproducible.Replace the unstability and uncertainty of conventional centrifugal separation, and greatly reduces the loss of bacterium.Easy to operate, high degree of automation simultaneously.Furthermore it is possible to by dimensional effect, separate to the property of can choose required enteric microorganism by using the nucleopore membranes of different pore size, can be used for being accurately controlled the relative abundance of different strain.
Description
Technical field
The invention belongs to the separation technology fields of the field of medical instrument technology, especially intestinal contents.
Background technique
The human body intestinal canal microorganism moment with human growth, not only helps digest food, and even more human body is effective against the external world
The barrier of pathogen invasion.The intestine microenvironment for the balance and stability that very long evolutionary process is formed for human body nutrition,
It is metabolized and is immunized and play a crucial role.There are a large amount of symbiotic microorganisms in human body, they largely reside in the intestines of people
In road, quantity is more than 1,000,000,000,000,000 (1014The order of magnitude), it is 10 times or more of human body cell sum.In very long evolutionary process,
Enteric microorganism and the mankind have reached good cooperation, immune play a crucial role to the nutrition of human body, metabolism and all.
Functional flora in healthy human faecal mass is transplanted to trouble as the effective means for rebuilding intestinal flora by caprophyl transplanting
In person's gastrointestinal tract, new intestinal flora balance is rebuild, realizes the treatment of enteron aisle and parenterally disease.A variety of floras are had been used at present
Diseases related treatment and pilot study, and be considered as breakthrough Advances in Medicine in recent years.But to divide from excrement
From caprophyl, what it is for medical staff is huge challenge.Therefore, design a kind of automation, standardized caprophyl separation and return
Receipts system, has very important significance.
Currently, automating separation and recovery system for caprophyl, other impurity are filtered to remove using multi-stag, the country is existing
Some literature reports, such as CN103330961B, CN105624024B and CN204097475U.But these caprophyls separation and
Recovery system obtains bacterium solution after being all filtered separation only for the impurity in excrement solution, makes subsequently through centrifuge centrifugation
Solution layering, outwells supernatant to retain useful flora.This collection handles collection method, operation be inconvenient and also due to
The limitation of technology used causes many small and light bacteriums to be disposed with supernatant, and there are also a large amount of excrement in reservation species
Just residue (more than 40 μm or more), and most of enteric bacteria size is less than 5 μm.And due to the diversity of fecal specimens and again
Polygamy and the otherness that everyone operates it is as a result uncontrollable so that this method otherness is very big, cannot achieve it is standardized can
Repetitive operation.In addition, these methods caprophyl obtained is all mixing, the micro- life of specific enteron aisle can not be selectively sieved
Object will accurately treat intestines problem for future, and be unable to satisfy.
Nucleopore membranes are to be punched to form with chemical etching reaming on megohmite insulant film by heavy ion, are a kind of secondary filters
With the ideal filter membrane of sieve particle, microcellular structure is cylindrical vent and pore size is uniform, belongs to screening filtration mechanism, when filtering
It has good selectivity all particle entrapments greater than aperture, bearing capacity on the surface of filter membrane as sieve
By force, high temperature-resistant disinfected, chemistry and biological stability are good.But in the prior art, the application of nucleopore membranes is provided to remove water
In impurity and harmful microorganism, finally realize the effect to purify water.What is shut off in nucleopore membranes is useless impurity.And it needs
The solution of purification is free of and a large amount of impurity, and bacterial content is relatively fewer, and filtering technique difficulty is little.Bacterium in enteron aisle accounts for whole
40% or more of a intestinal contents, the solution muddiness degree of content are far longer than the filtration application of common nucleopore membranes.This hair
The bright filtering feature that nucleopore membranes are creatively utilized passes through the nucleopore of different pore size in the separation application of intestinal contents
Film filtering, collects different types of intestinal contents, what is retained in nucleopore membranes is all the product of desired acquisition.
Summary of the invention
In order to solve the above-mentioned technical problem, the present invention is efficient, accurate, controllable to realize by the way of nucleopore membranes filtering
Specific intestinal contents are collected on ground, and specific technical solution is as follows:
The application of a kind of nucleopore membranes in intestinal contents separation, it is characterised in that: the intestinal contents separation uses
Nucleopore membranes.
Further, the aperture of the nucleopore membranes is 0.01-40 μm, preferably 0.5-10 μm.
Further, the nucleopore membranes are a tunic.
Further, the nucleopore membranes are two layers or two layers or more film, and the nucleopore membranes of different pore size are according to the object that shuts off
It is required that aperture is to small from being sequentially arranged greatly, for collecting various sizes of intestinal contents.
Further, the intestinal contents are enteric microorganism, including Bifidobacterium, Bacillus acidi lactici, Escherichia coli, intestines
Coccus etc., the size of the enteric microorganism is between 0.1 μm -40 μm, and preferably 0.5 μm -10 μm.
Further, the nucleopore membranes are the downward cone of the conical surface, and the nucleopore membranes are propped up by the cone of adjoining dimensions
The conical surface of frame support, the conical stent is equipped with hole；Or the nucleopore membranes are that cylinder is barrel-shaped, the nucleopore membranes are by ruler
The very little barrel-shaped bracket support of similar cylinder, the side and bottom surface of the barrel-shaped bracket of cylinder are equipped with hole.
Further, the nucleopore membranes are rotated by axis of the vertical central axis of circular cone or cylinder barrel.
Further, apply negative pressure under the nucleopore membranes.
Further, further include removal step before carrying out intestinal contents separation, remove large scale impurity.
Further, the intestinal contents separation carries out under nitrogen protection, to guarantee that the maximum of anaerobic bacteria survives
Rate.
Further, application of the nucleopore membranes in intestinal contents separation, it is characterised in that: the enteron aisle content
It can be directly placed into freeze dryer after object separation, be lyophilized into powder.
The beneficial technical effect of the present invention lies in: 1) nucleopore membranes point that using side wall filtering and rotating separation technology are combined
From enteric microorganism is extracted, traditional centrifugal isolation technics is substituted, can make extraction process more accurate controllable first, separation
It is more efficient while easy to operate, high degree of automation, furthermore it is possible to pass through size by using the nucleopore membranes of different pore size
Effect is optionally sequestered required enteric microorganism, for being accurately controlled the relative abundance of different strain；2) nitrogen is used
Gas shielded can ensure the survival of anaerobic type enteric microorganism during the extraction process.
Detailed description of the invention
Fig. 1 is the application in embodiment 1 by the nucleopore membranes in enteric microorganism separation, is received on nucleopore membrane mesh
The product 2g solid collected the object that shuts off is dissolved in 25ml physiological saline, the photo under 400 power microscopes.
Fig. 2 is that 2g solid precipitates in the lower sediment thing obtained in comparative example by the method that conventional centrifugal separates
Object is dissolved in 25ml physiological saline, the photo under 400 power microscopes.
Specific embodiment
Embodiment 1
A kind of application of nucleopore membranes in enteric microorganism separation, using single layer nucleopore membrane mesh, nucleopore membrane aperture is 0.5
μm.Nucleopore membranes are cone, and are supported by the conical stent of adjoining dimensions that the side and bottom surface of bracket are equipped with hole, are used
In through filtered water.Nucleopore membranes and bracket are rotated by axis of container vertical central axis, revolving speed 800rpm, and in every layer of core
Apply negative pressure under pore membrane, negative pressure value is 0.5 atmospheric pressure, for accelerating the separation of microorganism and water.Excrement after removal of impurities
Aqueous dispersions pass through nucleopore membranes, and after separation, the collected solid object that shuts off takes 2g to be dissolved in 25ml physiology on nucleopore membrane mesh
In salt water, observed under 400 power microscopes, as shown in Figure 1.
Comparative example
A kind of enteric microorganism separation method, the excrement suspension after removal of impurities carry out the micro- life of enteron aisle using centrifugation
The separation of object, 1500rpm, 3 minutes, in triplicate.Final 2g solid sediment is taken to be dissolved in 25ml physiological saline, 400
It is observed under power microscope, as shown in Figure 2.Figure it is seen that in the lower sediment thing being collected into, content of microorganisms
It is very low.Therefore, the effect of centrifuge separation collection microorganism is poor, since content of microorganisms is very low in product, so that therapeutic effect
It is bad.
As it can be seen that embodiment 1, compared with comparative example, extraction process is more accurate controllable, and separative efficiency is high, obtained
In product, the concentration of microorganism is higher, while easy to operate, high degree of automation.
Embodiment 2
A kind of application of nucleopore membranes in enteric microorganism separation, nucleopore membranes are the nucleopore membrane mesh of three layers of different pore size,
According to aperture from being sequentially arranged in a reservoir to small greatly, nucleopore membrane aperture is respectively 5 μm, 1.5 μm, 0.5 μm.Three layers of nucleopore membranes are equal
It is barrel-shaped for cylinder, and supported by the barrel-shaped bracket of the cylinder of adjoining dimensions, the side and bottom surface of bracket are equipped with hole, for penetrating
Filtered water.Nucleopore membranes and bracket are rotated by axis of container vertical central axis, revolving speed 800rpm, and under every layer of nucleopore membranes
Apply negative pressure, negative pressure value is 0.5 atmospheric pressure, for accelerating the separation of microorganism and water.Fecal water after removal of impurities dissipates
Liquid passes through nucleopore membranes, and various sizes of enteric microorganism is trapped in three layers of nucleopore membranes by filtering respectively, wherein 5 μm of nucleopore membranes
What is be mainly collected into is spirillum, and what 1.5 μm of nucleopore membranes were mainly collected into is bacillus, and what 0.5 μm of nucleopore membranes was mainly collected into is
Coccus.
As it can be seen that embodiment 2, compared with comparative example, in addition to extraction process is more accurate controllable, separative efficiency is high, simultaneously
It is easy to operate, other than high degree of automation, by using the nucleopore membranes of different pore size, by dimensional effect, the property of can choose
Enteric microorganism required for separating can be used for accurately treating not for being accurately controlled the relative abundance of different strain
The intestines problem of same type.
Embodiments of the present invention have been described in detail above, but also only to facilitate understand and explanation and illustrate, do not answer
It is considered as limitation of the present invention.The staff of any technical field can implement preferably according to the technique and scheme of the present invention
Case study on implementation, but it is all these change belong to scope of the presently claimed invention.
Claims (11)
1. a kind of application of nucleopore membranes in intestinal contents separation, it is characterised in that: the intestinal contents separation uses core
Pore membrane.
2. application of the nucleopore membranes as described in claim 1 in intestinal contents separation, it is characterised in that: the nucleopore membranes
Aperture is 0.01-40 μm, preferably 0.5-10 μm.
3. application of the nucleopore membranes as described in claim 1 in intestinal contents separation, it is characterised in that: the nucleopore membranes are
One layer of filter membrane.
4. application of the nucleopore membranes as described in claim 1 in intestinal contents separation, it is characterised in that: the nucleopore membranes are
Two layers or two layers or more filter membrane, the nucleopore membranes of different pore size are according to the requirement aperture to the object that shuts off from being sequentially arranged to small greatly.
5. application of the nucleopore membranes as described in claim 1 in intestinal contents separation, it is characterised in that: the enteron aisle content
Object is enteric microorganism, including Bifidobacterium, Bacillus acidi lactici, Escherichia coli, enterococcus etc., and the size of the enteric microorganism exists
Between 0.1 μm -40 μm, preferably 0.5 μm -10 μm.
6. application of the nucleopore membranes as described in claim 1 in intestinal contents separation, it is characterised in that: the nucleopore membranes are
The downward cone of the conical surface, the nucleopore membranes are supported by the conical stent of adjoining dimensions, on the conical surface of the conical stent
Equipped with hole；Or the nucleopore membranes are that cylinder is barrel-shaped, the nucleopore membranes are supported by the barrel-shaped bracket of cylinder of adjoining dimensions, described
The side and bottom surface of the barrel-shaped bracket of cylinder are equipped with hole.
7. nucleopore membranes as claimed in claim 6 intestinal contents separation in application, it is characterised in that: the nucleopore membranes with
The vertical central axis of circular cone or cylinder barrel is axis rotation.
8. application of the nucleopore membranes as described in claim 1 in intestinal contents separation, it is characterised in that: in the nucleopore membranes
Lower application negative pressure.
9. application of the nucleopore membranes as described in claim 1 in intestinal contents separation, it is characterised in that: carrying out in enteron aisle
It further include removal step before tolerant separation.
10. application of the nucleopore membranes as described in claim 1 in intestinal contents separation, it is characterised in that: in the enteron aisle
Tolerant separation carries out under nitrogen protection.
11. application of the nucleopore membranes as described in claim 1 in intestinal contents separation, it is characterised in that: in the enteron aisle
It is directly placed into freeze dryer after tolerant separation, is lyophilized into powder.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810851952.2A CN108949631A (en) | 2018-07-30 | 2018-07-30 | A kind of application of nucleopore membranes in intestinal contents separation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201810851952.2A CN108949631A (en) | 2018-07-30 | 2018-07-30 | A kind of application of nucleopore membranes in intestinal contents separation |
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| CN108949631A true CN108949631A (en) | 2018-12-07 |
Family
ID=64466206
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| CN201810851952.2A Pending CN108949631A (en) | 2018-07-30 | 2018-07-30 | A kind of application of nucleopore membranes in intestinal contents separation |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050244943A1 (en) * | 2004-03-15 | 2005-11-03 | Ladisch Michael R | Cell concentration and pathogen recovery |
| CN101952457A (en) * | 2007-12-21 | 2011-01-19 | 3M创新有限公司 | The department of microbiology of the fluid sample analysis method of unifying |
| CN102459567A (en) * | 2009-06-18 | 2012-05-16 | 默克专利股份公司 | Method for isolating cells |
| CN103725670A (en) * | 2012-10-15 | 2014-04-16 | 深圳华大基因科技有限公司 | Method for extracting nucleic acid from biological sample |
| CN107158781A (en) * | 2017-07-01 | 2017-09-15 | 上海谷奇核孔膜材料科技有限公司 | Liquid precise filter |
| CN107949391A (en) * | 2015-05-14 | 2018-04-20 | 克雷斯顿沃控股公司 | For faecal microbiota transplanting composition and be used to prepare and using they method and for delivering their device |
| CN108676704A (en) * | 2018-07-30 | 2018-10-19 | 骆奇 | A kind of intestinal contents separating and reclaiming device, method and products thereof |
-
2018
- 2018-07-30 CN CN201810851952.2A patent/CN108949631A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050244943A1 (en) * | 2004-03-15 | 2005-11-03 | Ladisch Michael R | Cell concentration and pathogen recovery |
| CN101952457A (en) * | 2007-12-21 | 2011-01-19 | 3M创新有限公司 | The department of microbiology of the fluid sample analysis method of unifying |
| US20110143334A1 (en) * | 2007-12-21 | 2011-06-16 | 3M Innovative Properties Company | Microbiological systems and methods of fluid sample analysis |
| CN102459567A (en) * | 2009-06-18 | 2012-05-16 | 默克专利股份公司 | Method for isolating cells |
| CN103725670A (en) * | 2012-10-15 | 2014-04-16 | 深圳华大基因科技有限公司 | Method for extracting nucleic acid from biological sample |
| CN107949391A (en) * | 2015-05-14 | 2018-04-20 | 克雷斯顿沃控股公司 | For faecal microbiota transplanting composition and be used to prepare and using they method and for delivering their device |
| CN107158781A (en) * | 2017-07-01 | 2017-09-15 | 上海谷奇核孔膜材料科技有限公司 | Liquid precise filter |
| CN108676704A (en) * | 2018-07-30 | 2018-10-19 | 骆奇 | A kind of intestinal contents separating and reclaiming device, method and products thereof |
Non-Patent Citations (8)
| Title |
|---|
| M.L. TORTORELLO等: "Rapid identification of Escherichia coli 0157:H7 in bovine feces using the antibody-direct epifluorescent filter technique ( Ab-DEFT)", 《VETERINARY MICROBIOLOGY》 * |
| M.L. TORTORELLO等: "Rapid identification of Escherichia coli 0157:H7 in bovine feces using the antibody-direct epifluorescent filter technique ( Ab-DEFT)", 《VETERINARY MICROBIOLOGY》, vol. 51, 31 December 1996 (1996-12-31), pages 7610 - 7617 * |
| MARIA C. PORTILLO等: "Cell Size Distributions of Soil Bacterial and Archaeal Taxa", 《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》 * |
| MARIA C. PORTILLO等: "Cell Size Distributions of Soil Bacterial and Archaeal Taxa", 《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》, vol. 79, 31 December 2013 (2013-12-31), pages 343 - 349 * |
| 王守忠等: "新型的啤酒除菌方法――核孔膜过滤", 《酿酒》 * |
| 王守忠等: "新型的啤酒除菌方法――核孔膜过滤", 《酿酒》, no. 01, 31 December 1988 (1988-12-31), pages 31 - 37 * |
| 祝洪山: "滤膜过滤清除溶液中的细菌", 《国外医学.卫生学分册》 * |
| 祝洪山: "滤膜过滤清除溶液中的细菌", 《国外医学.卫生学分册》, no. 06, 25 November 1993 (1993-11-25), pages 46 - 47 * |
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