Disclosure of Invention
In order to overcome the defects of the prior art, the first aim of the invention is to provide a preparation method of small molecular group water, which has the advantages of simple process and low production cost, and the prepared small molecular group water has good stability.
The second purpose of the invention is to provide small molecular group water which has good stability.
A third object of the present invention is to provide a use of small molecular group water.
In order to achieve the first object of the invention, the present invention adopts the following technical scheme:
the invention provides a preparation method of small molecular group water, which comprises the following steps:
collecting fog: collecting mist water by high-pressure concentration;
resin filtration: passing the mist through an ion resin exchange layer;
and (3) filtering by using activated carbon: the water coming out of the ion resin exchange layer passes through the active carbon layer;
reverse osmosis treatment: the water coming out of the active carbon layer passes through a reverse osmosis membrane;
nuclear magnetic resonance treatment: and (3) treating the water coming out of the reverse osmosis membrane by using a nuclear magnetic resonance apparatus to obtain the small molecular group water.
Further, in the mist water collecting step, the operation of high-pressure concentration is to concentrate air at high pressure by adopting a dehumidifier. The dehumidifier is also called a dehumidifier and consists of a compressor, a heat exchanger, a fan, a water container, a shell and a controller. The working principle of the dehumidifier is as follows: the moisture is condensed on the low-temperature surface so as to collect condensed water, when the device is operated, the fan pumps moist air into the machine, water molecules in the air are condensed into water drops through the heat exchanger, and the water drops are collected by the water container so as to obtain fog.
Further, in the step of collecting the mist, the collected mist is the mist in the air of the lake surface or the lake side. The water mist in the air on the lake surface or the lake side has smaller impurities and higher pollution-free degree, is more suitable for preparing the small molecular group water, and is also more suitable for extracting plant polysaccharide.
Further, the mist water sequentially passes through the ion resin exchange layer, the activated carbon layer and the reverse osmosis membrane by using a high-pressure pump; wherein, the high-pressure pump is as the power transmission device of fog water, can make fog water pass ion resin exchange layer, activated carbon layer and reverse osmosis membrane easily.
Further, the pressure of the high-pressure pump is 10-15 times of the normal pressure value. The pressure can enable mist water to better pass through the ion resin exchange layer, the activated carbon layer and the reverse osmosis membrane.
Further, in the resin filtration step, the ion resin exchange layer includes a cation resin exchange layer. Wherein the cation resin exchange layer is mainly used for reducing the hardness in fog water, namely Ca in water is removed by the cation resin exchange layer 2+ 、Mg 2+ And then the metal ion impurities in the fog water are removed.
Further, in the activated carbon filtering step, the activated carbon layer comprises a macroporous activated carbon layer and a small-pore activated carbon layer which are sequentially arranged; wherein, the macroporous active carbon layer is convenient for mist water to be led in and filter impurities, and the small pore active carbon layer is convenient for reducing the number of water molecules of water molecular groups.
Further, the aperture of the macroporous active carbon layer is 100 nm-10000 nm, and the aperture of the small-pore active carbon layer is 0.3 nm-2 nm. Wherein, the aperture is the aperture active carbon layer of 0.3nm ~ 2nm, under the drive transmission effect of high-pressure pump, can reduce the hydrone quantity of hydrone group well. Currently, water clusters consisting of less than 10 water molecules are generally referred to as small clusters, and the diameter of each water molecule is 0.4nm. When the mist water filtered by the resin filtration and the macroporous active carbon layer passes through the small-pore active carbon layer with the aperture of 0.3-2 nm under the drive of the high-pressure pump, the number of water molecules of the water molecule group can be reduced, and the mist water coming out of the small-pore active carbon layer is small-molecular group water.
Further, in the reverse osmosis treatment step, the pore diameter of the reverse osmosis membrane is 0.3 nm-2 nm. Mist water passes through the reverse osmosis membrane after passing through the active carbon layer, so that the number of water molecules of the water molecule groups can be further reduced.
Further, in the nuclear magnetic resonance treatment step, the treatment time by using a nuclear magnetic resonance apparatus is 1.5-2.5 hours; the nuclear magnetic resonance frequency is 200-400 HMz. The nuclear magnetic resonance treatment has the effects that on one hand, the number of water molecules of the water molecule groups can be further reduced, and on the other hand, the small molecular group water obtained after the nuclear magnetic resonance treatment has good stability.
Wherein, due to the synergistic effect of mist water collection, resin filtration, active carbon filtration, reverse osmosis treatment and nuclear magnetic resonance treatment in the preparation process, the small molecular group water can be prepared, and the prepared small molecular group water has better stability.
In order to achieve the second object of the invention, the present invention adopts the following technical scheme:
the invention provides small molecular group water which is prepared by the preparation method of the small molecular group water. The small molecular group water prepared by the preparation method has good stability due to the synergistic effect of resin filtration, active carbon filtration, reverse osmosis treatment and nuclear magnetic resonance treatment in the preparation process, and can be stored for 12 months.
In order to achieve the third object of the present invention, the present invention adopts the following technical scheme:
the invention provides application of small molecular group water, which is applied to extraction of plant polysaccharide. Because the water of the small molecular group has better dissolving capacity and penetrating power than common tap water or deionized water, the dissolving capacity of the plant polysaccharide can be more and the dissolving variety of the plant polysaccharide can be more abundant when the water is applied to extracting the plant polysaccharide.
Further, the plant polysaccharide is prepared from the raw materials including the small molecular group water and the to-be-extracted matter; the extract comprises radix Scutellariae, poria and Lentinus Edodes; and/or
The plant polysaccharide comprises the following raw materials in percentage by weight:
the plant polysaccharide is prepared into a product for resisting respiratory diseases by using small molecular group water.
The invention selects different plant tissues to mix for extracting plant polysaccharide, and can improve the immunity of human body and the functions of bacteriostasis and antivirus by the respective special performances of different polysaccharide molecules in the baical skullcap root, the white poria and the lentinus edodes. Wherein, fresh radix scutellariae, white poria cocos and mushroom can be directly ground or smashed into homogenate, and dried radix scutellariae, white poria cocos and mushroom can be directly smashed into powder. In addition, the small molecular group water can also avoid the plant polysaccharide from being too viscous in the extraction process, thereby improving the extraction rate of the polysaccharide. In addition, when the product for resisting the respiratory diseases is prepared, the extracted plant polysaccharide is dissolved by using the small molecular group water as a solvent, so that the product for resisting the respiratory diseases is prepared, and the plant polysaccharide dissolved by the small molecular group water can enter a human body more easily, and has better functions of resisting viruses and improving immunity.
Compared with the prior art, the invention has the beneficial effects that:
(1) The preparation method of the small molecular group water is to prepare the small molecular group water through mist water collection, resin filtration, active carbon filtration, reverse osmosis treatment and nuclear magnetic resonance treatment, wherein the mist water is adopted as a preparation raw material, so that the water source has smaller impurities, higher pollution-free degree and higher purification degree, and is more beneficial to preparing the small molecular group water. Wherein the resin filtration step is capable of removing impurities and contaminants in the mist water, particularly metal ion impurities such as Ca 2+ 、Mg 2+ . And in the step of filtering by the activated carbon, impurities and pollutants in defogging water can be further removed on the one hand, and the number of water molecules in water molecule groups can be reduced on the other hand. And the reverse osmosis treatment step can further remove impurities, pollutants and bacteria in water and further reduce the number of water molecules in the water molecular groups. The nuclear magnetic resonance treatment step can further reduce the number of water molecules in the water molecule group, and the stability of the obtained small molecule group water is better. Therefore, due to the synergistic effect of mist water collection, resin filtration, active carbon filtration, reverse osmosis treatment and nuclear magnetic resonance treatment in the preparation process, the small molecular group water can be prepared, and the prepared small molecular group water has good stability and can be stored for 12 months.
(2) The preparation method of the small molecular group water has the characteristics of simple process, low production cost and suitability for large-scale production.
(3) The small molecular group water is prepared by the synergistic effect of mist water collection, resin filtration, active carbon filtration, reverse osmosis treatment and nuclear magnetic resonance treatment, so that the prepared small molecular group water has better stability.
(4) The application of the small molecular group water is applied to extracting plant polysaccharide. Because the water of the small molecular group has better dissolving capacity and penetrating power than common tap water or deionized water, the dissolving capacity of the plant polysaccharide can be more and the dissolving variety of the plant polysaccharide can be more abundant when the water is applied to extracting the plant polysaccharide.
Detailed Description
In order to make the technical problems, technical schemes and beneficial effects to be solved more clear, the invention is further described in detail below with reference to the embodiments. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
The terminology used in the embodiments of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used in this specification, the singular forms "a", "an", and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise.
In the embodiment of the invention, the preparation method of the small molecular group water comprises the following steps:
collecting fog: collecting mist water by high-pressure concentration;
resin filtration: passing the mist through an ion resin exchange layer;
and (3) filtering by using activated carbon: the water coming out of the ion resin exchange layer passes through the active carbon layer;
reverse osmosis treatment: the water coming out of the active carbon layer passes through a reverse osmosis membrane;
nuclear magnetic resonance treatment: and (3) treating the water coming out of the reverse osmosis membrane by using a nuclear magnetic resonance apparatus to obtain the small molecular group water.
Wherein, adopt fog as the preparation raw materials, can make the impurity of water source littleer, pollution-free degree is higher, and the purification degree is higher, more does benefit to the preparation small molecule group water. Wherein, the resin filtering step can remove impurities and pollutants in the mist water, in particular to remove impurity ions in the mist water. And in the step of filtering by the activated carbon, impurities and pollutants in defogging water can be further removed on the one hand, and the number of water molecules in water molecule groups can be reduced on the other hand. And the reverse osmosis treatment step can further remove impurities, pollutants and bacteria in water and further reduce the number of water molecules in the water molecular groups. The nuclear magnetic resonance treatment step can further reduce the number of water molecules in the water molecule group, and the stability of the obtained small molecule group water is better. Therefore, due to the synergistic effect of mist water collection, resin filtration, active carbon filtration, reverse osmosis treatment and nuclear magnetic resonance treatment in the preparation process, the small molecular group water can be prepared, and the prepared small molecular group water has good stability and can be stored for 12 months.
In the embodiment of the invention, in the mist water collecting step, the operation of high-pressure concentration is to adopt a dehumidifier to concentrate air at high pressure; the dehumidifier is also called a dehumidifier and consists of a compressor, a heat exchanger, a fan, a water container, a shell and a controller. The working principle of the dehumidifier is as follows: the moisture is condensed on the low-temperature surface so as to collect condensed water, when the device is operated, the fan pumps moist air into the machine, water molecules in the air are condensed into water drops through the heat exchanger, and the water drops are collected by the water container so as to obtain fog.
In the embodiment of the invention, in the step of collecting the fog, the collected fog is the fog in the air of the lake surface or the lake side. The water mist in the air on the lake surface or the lake side has smaller impurities and higher pollution-free degree, is more suitable for preparing the small molecular group water, and is also more suitable for extracting plant polysaccharide.
In the embodiment of the invention, mist water sequentially passes through an ion resin exchange layer, an active carbon layer and a reverse osmosis membrane by utilizing a high-pressure pump; wherein, the high-pressure pump is as the power transmission device of fog water, can make fog water pass ion resin exchange layer, activated carbon layer and reverse osmosis membrane easily.
In the embodiment of the invention, the pressure of the high-pressure pump is 10-15 times of the pressure value of the normal pressure. The pressure can enable mist water to better pass through the ion resin exchange layer, the activated carbon layer and the reverse osmosis membrane.
In the embodiment of the invention, in the resin filtering step, the ion resin exchange layer comprises a cation resin exchange layer. Wherein the cation resin exchange layer is mainly used for reducing the hardness in fog water, namely Ca in water is removed by the cation resin exchange layer 2+ 、Mg 2+ And then the metal ion impurities in the fog water are removed.
In the embodiment of the invention, in the activated carbon filtering step, the activated carbon layer comprises a macroporous activated carbon layer and a small-pore activated carbon layer which are sequentially arranged; wherein, the macroporous active carbon layer is convenient for mist water to be led in and filter impurities, and the small pore active carbon layer is convenient for reducing the number of water molecules of water molecular groups.
In the embodiment of the invention, the aperture of the macroporous active carbon layer is 100 nm-10000 nm, and the aperture of the small-pore active carbon layer is 0.3 nm-2 nm. Wherein, the aperture is the aperture active carbon layer of 0.3nm ~ 2nm, under the drive transmission effect of high-pressure pump, can reduce the hydrone quantity of hydrone group well. Currently, water clusters consisting of less than 10 water molecules are generally referred to as small clusters, and the diameter of each water molecule is 0.4nm. When the mist water filtered by the resin filtration and the macroporous active carbon layer passes through the small-pore active carbon layer with the aperture of 0.3-2 nm under the drive of the high-pressure pump, the number of water molecules of the water molecule group can be reduced, and the mist water coming out of the small-pore active carbon layer is small-molecular group water.
In the embodiment of the invention, in the reverse osmosis treatment step, the pore diameter of the reverse osmosis membrane is 0.3 nm-2 nm. Wherein, the reverse osmosis membrane with the aperture of 0.3 nm-2 nm can well cooperate to reduce the number of water molecules of the water molecule group under the drive transmission action of the high-pressure pump. When the small molecular group water coming out of the small pore active carbon layer enters into the reverse osmosis membrane with the aperture of 0.3 nm-2 nm, on one hand, the water molecular number of the water molecular group is further enhanced to be less than 10, and on the other hand, the water molecular number of the water molecular group can be further reduced by the pores with smaller aperture in the reverse osmosis membrane.
In the embodiment of the invention, in the nuclear magnetic resonance treatment step, the treatment time by using a nuclear magnetic resonance apparatus is 1.5-2.5 hours; the nuclear magnetic resonance frequency is 200-400 HMz. The nuclear magnetic resonance treatment has the effects that on one hand, the number of water molecules of the water molecule groups can be further reduced, and on the other hand, the small molecular group water obtained after the nuclear magnetic resonance treatment has good stability.
In the embodiment of the invention, the small molecular group water is prepared by the preparation method of the small molecular group water. The small molecular group water prepared by the preparation method has good stability due to the synergistic effect of resin filtration, active carbon filtration, reverse osmosis treatment and nuclear magnetic resonance treatment in the preparation process, and can be stored for 12 months.
In the embodiment of the invention, the application of the small molecular group water is applied to the extraction of plant polysaccharide. Because the water of the small molecular group has better dissolving capacity and penetrating power than common tap water or deionized water, the dissolving capacity of the plant polysaccharide can be more and the dissolving variety of the plant polysaccharide can be more abundant when the water is applied to extracting the plant polysaccharide.
In the embodiment of the invention, the plant polysaccharide is prepared from the following raw materials of small molecular group water and a to-be-extracted substance; the extract comprises Scutellariae radix, poria and Lentinus Edodes;
the plant polysaccharide comprises the following raw materials in percentage by weight:
the plant polysaccharide is prepared into respiratory disease resisting product through small molecular group water.
Because the types of polysaccharide contained in the baical skullcap root, the white poria and the lentinus edodes to be extracted are different, the invention selects different plant tissues to mix for extracting plant polysaccharide, and the functions of improving the immunity and bacteriostasis and antivirus of human body can be improved through the respective unique properties of different polysaccharide molecules in the baical skullcap root, the white poria and the lentinus edodes. Wherein, fresh radix scutellariae, white poria cocos and mushroom can be directly ground or smashed into homogenate, and dried radix scutellariae, white poria cocos and mushroom can be directly smashed into powder. In addition, the small molecular group water can also avoid the plant polysaccharide from being too viscous in the extraction process, thereby improving the extraction rate of the polysaccharide. In addition, when the product for resisting the respiratory diseases is prepared, the extracted plant polysaccharide is dissolved by using the small molecular group water as a solvent, so that the product for resisting the respiratory diseases is prepared, and the plant polysaccharide dissolved by the small molecular group water can enter a human body more easily, and has better functions of resisting viruses and improving immunity.
The following description is made with reference to specific embodiments.
Example 1
The preparation method of the small molecular group water comprises the following steps:
collecting fog: collecting mist water by high-pressure concentration; wherein, the operation of high-pressure concentration is to use a dehumidifier to concentrate air at high pressure; in this embodiment, the collected mist is the mist in the air around the lake;
resin filtration: passing the mist through an ion resin exchange layer; in this embodiment, the ion-resin exchange layer includes a cation-resin exchange layer;
and (3) filtering by using activated carbon: the water coming out of the ion resin exchange layer passes through the active carbon layer; in this embodiment, the activated carbon layer includes a macroporous activated carbon layer and a microporous activated carbon layer that are sequentially disposed; in this example, the pore diameter of the macroporous active carbon layer is 100nm to 10000nm, and the pore diameter of the small pore active carbon layer is 0.3nm to 2nm.
Reverse osmosis treatment: the water coming out of the active carbon layer passes through a reverse osmosis membrane; in this example, the pore diameter of the reverse osmosis membrane is 0.3nm to 2nm.
Nuclear magnetic resonance treatment: and (3) treating the water coming out of the reverse osmosis membrane by using a nuclear magnetic resonance apparatus to obtain the small molecular group water. In this embodiment, the treatment time by using the nmr is 2 hours; the frequency of nuclear magnetic resonance was 300HMz.
The mist water sequentially passes through the ion resin exchange layer, the activated carbon layer and the reverse osmosis membrane by utilizing a high-pressure pump; in this embodiment, the pressure of the high-pressure pump is 13 times the value of the normal pressure.
Example 2
The difference between this embodiment and embodiment 1 is that in this embodiment, the collected mist is the mist in the air of the lake surface; in this example, the treatment time by the nmr was 1.5 hours; the frequency of nuclear magnetic resonance was 400HMz. In this embodiment, the pressure of the high-pressure pump is 10 times the value of the normal pressure. The remaining technical scheme and preparation steps of this example are the same as those of example 1.
Example 3
The difference between this embodiment and embodiment 1 is that in this embodiment, the collected mist is the mist in the air of the lake; in this embodiment, the treatment time by using the nmr is 2.5 hours; the frequency of nuclear magnetic resonance was 200HMz. In this embodiment, the pressure of the high-pressure pump is 15 times the value of the normal pressure. The remaining technical scheme and preparation steps of this example are the same as those of example 1.
Example 4
The difference between this embodiment and embodiment 1 is that in this embodiment, the collected mist is the mist in the air of the lake surface; in this embodiment, the treatment time by using the nuclear magnetic resonance apparatus is 1.8 hours; the frequency of nuclear magnetic resonance was 350HMz. In this embodiment, the pressure of the high-pressure pump is 11 times the value of the normal pressure. The remaining technical scheme and preparation steps of this example are the same as those of example 1.
Example 5
The difference between this embodiment and embodiment 1 is that in this embodiment, the collected mist is the mist in the air of the lake; in this embodiment, the treatment time by using the nmr is 2.2 hours; the frequency of nuclear magnetic resonance was 250HMz. In this embodiment, the pressure of the high-pressure pump is 14 times the atmospheric pressure value. The remaining technical scheme and preparation steps of this example are the same as those of example 1.
Example 6
A small molecular group water is prepared by the preparation method of the small molecular group water in the embodiment 1.
Example 7
The application of the small molecular group water is characterized in that the small molecular group water in the embodiment 6 is applied to the extraction of plant polysaccharide.
The plant polysaccharide is prepared from small molecular group water and extract; the extract comprises Scutellariae radix, poria and Lentinus Edodes;
in this embodiment, the plant polysaccharide comprises the following raw materials in percentage by weight:
the plant polysaccharide is prepared into respiratory disease resisting product through small molecular group water.
Example 8
The application of the small molecular group water is characterized in that the small molecular group water in the embodiment 6 is applied to the extraction of plant polysaccharide.
The plant polysaccharide is prepared from small molecular group water and extract; the extract comprises Scutellariae radix, poria and Lentinus Edodes;
in this embodiment, the plant polysaccharide comprises the following raw materials in percentage by weight:
the plant polysaccharide is prepared into respiratory disease resisting product through small molecular group water.
Example 9
The application of the small molecular group water is characterized in that the small molecular group water in the embodiment 6 is applied to the extraction of plant polysaccharide.
The plant polysaccharide is prepared from small molecular group water and extract; the extract comprises Scutellariae radix, poria and Lentinus Edodes;
in this embodiment, the plant polysaccharide comprises the following raw materials in percentage by weight:
the plant polysaccharide is prepared into respiratory disease resisting product through small molecular group water.
Example 10
The application of the small molecular group water is characterized in that the small molecular group water in the embodiment 6 is applied to the extraction of plant polysaccharide.
The plant polysaccharide is prepared from small molecular group water and extract; the extract comprises Scutellariae radix, poria and Lentinus Edodes;
in this embodiment, the plant polysaccharide comprises the following raw materials in percentage by weight:
the plant polysaccharide is prepared into respiratory disease resisting product through small molecular group water.
Comparative example 1
The difference between the plant polysaccharide and the comparative example 7 is that the comparative example uses purified water instead of the small molecular group water of the example 7. Wherein the purified water is also called deionized water, i.e. non-small molecular group water. The remaining technical scheme and preparation procedure of this comparative example are the same as in example 7.
Experimental test:
(1) Antiviral testing
A plant polysaccharide prepared in example 7 is diluted by small molecular group water to prepare a plant polysaccharide solution with the concentration of 0.5mg/mL, then a mixture of human adenovirus B7-enhanced green fluorescent protein (HAdV-B7-EGFP) and the prepared plant polysaccharide solution is smeared on the surface of a DMEM/F-12 culture medium to prepare test samples, wherein seven test samples with different polysaccharide concentrations are respectively prepared, and the mass volume concentration of the plant polysaccharide in the test samples is respectively 0.5mg/L, 0.6mg/L, 0.7mg/L, 0.8mg/L, 0.9mg/L, 1.0mg/L and 2.0mg/L, and the polysaccharide concentrations of the seven test samples are respectively recorded as 5%, 6%, 7%, 8%, 9%, 10% and 20% for convenience in recording. Wherein the concentration of HAdV-B7-EGFP in the seven test samples is 200TCID 50 0.1mL. Then, a virus control sample and a blank control sample were prepared, and nine test samples were taken in total. The virus control sample was a sample to which no plant polysaccharide was added with respect to the seven test samples, and the blank control sample was a sample to which no plant polysaccharide and HAdV-B7-EGFP were added with respect to the seven test samples.
Nine test samples prepared as described above were then placed in 96-well plates at 37℃and 5% CO 2 Incubating for 1h in an incubator; and single-layer adherent growth in 96-well plate, discarding culture medium with virus cell density of 90%, adding 100 μl of the above nine test samples to each well, respectively, setting 3 multiple wells for each test sample, and adding 5% CO at 37deg.C 2 Incubating for 2 hours in an incubator; the mixture of plant polysaccharide solution and HAdV-B7-EGFP was then discarded, 100. Mu.L of DMEM/F-12 medium was added to each well, and the mixture was then incubated at 37℃and 5% CO 2 Culturing in an incubator for 3 days. And then photographing the nine finally obtained samples by using an inverted microscope to obtain an inverted microscope image with antiviral effect comparison as shown in figure 1.
As can be seen from the test results in FIG. 1, the sample with the concentration of plant polysaccharide recorded as 10%, namely, the sample with the concentration of plant polysaccharide recorded as 1.0mg/L, can obviously inhibit virus infection. The sample with the plant polysaccharide concentration recorded as 20 percent, namely the sample with the plant polysaccharide concentration of 2.0mg/L, can completely inhibit virus infection.
Therefore, through the antiviral test, the plant polysaccharide prepared by the extraction method has good antiviral function.
(2) Antiviral testing with small molecule cluster water and purified water
Comparing the plant polysaccharide prepared in example 7 with the plant polysaccharide prepared in comparative example 1 according to the above-mentioned (1) antiviral test method, respectively preparing a small molecular group water sample and a purified water sample, wherein the concentration of the plant polysaccharide in the small molecular group water sample and the purified water sample is 1.0mg/L, and the concentration of the HAdV-B7-EGFP is 200TCID 50 0.1mL. And (2) photographing the small molecular group water sample and the purified water sample which are cultured according to the antiviral test method (1) by using an inverted microscope to obtain an inverted microscope image of the antiviral effect comparison of the plant polysaccharide shown in the figure 2.
From the test result graph of fig. 2, it can be seen that the application of the small molecular group water to the extracted plant polysaccharide has better antiviral effect than the application of the purified water to the extracted plant polysaccharide. Therefore, the small molecular group water has better dissolving power and penetrating power when extracting plant polysaccharide due to smaller molecular groups, and further more polysaccharide can be dissolved out from plant tissues, so that the extracted plant polysaccharide has better antiviral effect compared with the plant polysaccharide extracted by purified water.
(3) Immunity Performance test
The immunity performance test is to detect the influence of the plant polysaccharide solution prepared by the invention on the weight of the immune organs of mice.
The plant polysaccharide prepared in the example 7 is diluted by small molecular group water to prepare a plant polysaccharide solution with the concentration of 0.5mg/mL, and then the plant polysaccharide solution is diluted by physiological saline with the mass percent concentration of 0.9% to prepare test samples, wherein the test samples with the different polysaccharide concentrations are respectively prepared, the mass volume concentration of the plant polysaccharide in the test samples is respectively 1.0mg/L and 0.1mg/L, and the polysaccharide concentrations of the two test samples are respectively recorded as 10% and 1% for the convenience of recording. A blank was then prepared for a total of three test samples. The blank sample is physiological saline with the mass percentage concentration of 0.9 percent.
36 Kunming mice weighing 15-18 g are randomly divided into 3 groups, and 12 mice are selected from each group. Then, the above prepared 10% plant polysaccharide solution, 1% plant polysaccharide solution and blank control sample were used to continuously perfuse the above 3 groups of mice for one week, and the amount of the perfused stomach was 0.3mL/10g per day according to the weight of the mice, and were recorded as group A, group B and group C, respectively. Mice were then sacrificed 2h after the last administration of the lavage, and the weights of the mice, thymus and spleen were weighed, respectively, and the ratio of the weights (mg) of thymus and spleen to the weights (g) of thymus and spleen was used as an index of thymus and spleen.
The effect of the three test samples on the weight of the immune organs of the mice is shown in Table 1 below.
TABLE 1 results of test samples for effects on the weight of immune organs in mice
Group of
|
Weight/g
|
Thymus weight/mg
|
Thymus index/(mg/g)
|
Spleen weight/mg
|
Spleen index/(mg/g)
|
Group A
|
16.82±1.10
|
71.61
|
4.52±0.47
|
112.89
|
6.71±0.44
|
Group B
|
16.35±0.78
|
60.55
|
4.01±0.19
|
99.50
|
6.09±0.29
|
Group C
|
16.74±0.83
|
48.87
|
2.92±0.14
|
86.91
|
5.19±0.26 |
From the test results shown in Table 1, it was found that the test sample having a plant polysaccharide concentration of 10% had the greatest effect on the body weight, thymus mass and spleen mass of mice, and that the thymus mass and spleen mass were increased by about 23mg to 26mg relative to the normal saline control group. In addition, the test sample with the plant polysaccharide concentration of 1% has weight gain of 10-13 mg relative to the normal saline blank control group. Therefore, the large-dose plant polysaccharide solution has obvious influence on the weight gain of thymus and spleen of the mice, and the plant polysaccharide solution provided by the invention can improve the immunity performance of the mice. Therefore, the plant polysaccharide prepared by the invention can improve the immunity of human bodies and the functions of bacteriostasis and antivirus through the respective special performances of different polysaccharide molecules in the baical skullcap root, the white poria and the lentinus edodes. In addition, as the small molecular group water is adopted in the extraction of the plant polysaccharide and the small molecular group water is adopted in the preparation of the plant polysaccharide solution, the plant polysaccharide dissolved by the small molecular group water can enter the human body more easily, and the functions of resisting viruses and improving immunity are achieved.
The foregoing description of the preferred embodiments of the present application is not intended to be limiting, but is intended to cover any and all modifications, equivalents, and alternatives falling within the spirit and principles of the present application.