CN108949561A - Intelligent immunity cell culture oscillator - Google Patents
Intelligent immunity cell culture oscillator Download PDFInfo
- Publication number
- CN108949561A CN108949561A CN201810980295.1A CN201810980295A CN108949561A CN 108949561 A CN108949561 A CN 108949561A CN 201810980295 A CN201810980295 A CN 201810980295A CN 108949561 A CN108949561 A CN 108949561A
- Authority
- CN
- China
- Prior art keywords
- test tube
- buffer
- cell culture
- dashpot
- concussion plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
Abstract
The present invention provides a kind of intelligent immunity cell culture oscillator, including power device, concussion plate, mounting platform and controller, the power device includes motor, crankshaft and buffer, the motor driven crankshafts move in a circle, crankshaft drives buffer by connecting rod, buffer is fixed on concussion plate, and vibration back and forth has imitated the process of artificial concussion plus all standing of touching silica gel completely.It the beneficial effects of the invention are as follows due to the adoption of the above technical scheme, improves and effectively shakes effect in cell cultivation process, realize the automation and accuracy of oscillation frequency adjustment, improve the efficiency of cell culture.
Description
Technical field
The present invention relates to a kind of oscillator, a kind of particularly intelligent immunity cell culture oscillator.
Background technique
Tumor vaccine cells treatment is a kind of tumor treatment model emerging, with significant curative effect, but in immunocyte
Laboratory cultures during, it may appear that cell wall built-up and aggregation the problems such as, influence the amplification and quality of immunocyte.
In the prior art, the wall built-up of cell and rendezvous problem are mainly to be solved by the concussion to cell culture tube.
What oscillator on the market actually rose at present is stirring action.This stirring action to the wall built-up of immunocyte and
It is not significant to assemble at all useless or effect.Therefore, in immunocyte incubation, still laboratory technician is needed to shake by hand
It swings, leads to the unstable quality of immunocyte, influence the effect for the treatment of, and labor intensive, limit culture efficiency.
Summary of the invention
The object of the present invention is to provide a kind of intelligent immunity cell culture oscillators of automation, with concussion and soft silicon back and forth
The beating movement of manpower is imitated in glue stop；The journey of cell aggregation and wall built-up is judged automatically using laser scanning and micro processor
Degree automatically adjusts oscillation frequency.
The technical scheme is that
A kind of intelligent immunity cell culture oscillator, including power device, concussion plate, mounting platform and controller,
The power device includes motor, crankshaft and buffer, and the motor driven crankshafts move in a circle, and crankshaft passes through
Connecting rod drives buffer, and buffer is fixed on concussion plate；
The concussion plate upper surface is equipped with no less than 2 test tube fixed frames, and concussion plate lower surface is equipped with directive wheel and braking
Block, stopper tab two sides are equipped with braking silica gel, braking silica gel solid on the mounting platform, be additionally provided on the mounting platform with
The track that directive wheel matches；
The motor is electrically connected with the controller.
The power that the present invention utilizes motor to generate realizes the straight reciprocating motion of culture test tube by crankshaft and concussion plate,
Motor band dynamic crankshaft, crankshaft drive buffer, and buffer is fixed with concussion plate, to drive concussion plate.Also, the present invention is also set
Braking silica gel is set, this vibration back and forth has imitated the process of artificial concussion plus all standing of touching silica gel completely.
Preferably, power device further includes reduction gearing, and the adjusting of oscillation frequency is realized by reduction gearing.
The test tube fixed frame is equipped with test tube jack, and laser detector, the laser detection dress are equipped at test tube jack
Set laser beam emitting device and laser receiver including being located at test tube jack two sides, laser beam emitting device and laser receiver
It is electrically connected with the controller.
Using Laser emission and receive obtained light transmittance come the aggregation of discernable cell and wall built-up degree, controller according to
Laser light transmission rate determines oscillation frequency, if laser light transmission rate is high, illustrates that immunocyte aggregation and wall built-up be not serious, then slows down
Vibration frequency；The acceleration shock if light transmittance is low on the contrary.
Each test tube jack is on test tube receptacle axis direction is equipped with no less than 3 groups of laser detectors in parallel.Three groups
Laser detector can detect the different location of culture test tube, improve the accuracy of transmittance data, in addition, for training
Light transmittance in nutrient solution can permit a degree of light transmittance decline.And for the tube wall of not culture solution, it is not allow to exempt from
Existing for epidemic disease cell, multiple groups detection can be achieved to distinguish observation.
The top and bottom of the test tube jack are respectively equipped with silicone gasket.Silicone gasket plays protection to culture test tube and makees
With, prevent its because concussion with the direct collision of test tube fixed frame lead to breakage.
Buffer includes dashpot closed at both ends and and the sliding block that moves back and forth in dashpot, the sliding block and connecting rod
It is connected, the dashpot is connected with concussion plate, and the both ends of dashpot are equipped with buffer spring.Connecting rod band movable slider, sliding block hit bullet
Spring, spring drive dashpot, and dashpot is connected with concussion plate.The deformation quantity for braking silica gel itself is limited, it is desirable to whole device
Coordination operation, the motion frequency needs of the motion frequency of sliding block and stopper tab work in coordination.Sliding block is driven by the crankshaft, fortune
When the end of dynamic frequency, that is, stopper tab hits braking silica gel, the speed of sliding block is lower, dynamics deficiency is hit, at this point, buffering elastic
Spring just plays the role of energy storage and promotes concussion, and when sliding block is moved to side, part kinetic energy storage caused by sliding block is in slow
It rushes in spring, when stopper tab touches braking silica gel, the kinetic energy of buffer spring release storage drives dashpot and concussion plate
It is hit energetically with braking silica gel, improves concussion effect.
The advantages and positive effects of the present invention are: due to the adoption of the above technical scheme, improving cell cultivation process
In effectively shake effect, realize oscillation frequency adjustment automation and accuracy, improve the efficiency of cell culture.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of present invention concussion plate
Fig. 2 is the structural schematic diagram of power device under present invention concussion plate
Fig. 3 is the structural schematic diagram for shaking test tube fixed frame on plate
In figure:
1, plate 2, crankshaft 3, buffer are shaken
4, connecting rod 5, braking silica gel 6, track
7, shell 11, test tube fixed frame 12, directive wheel
13, stopper tab 31, dashpot 32, sliding block
33, buffer spring 111, laser beam emitting device 112, laser receiver
113, silicone gasket
Specific embodiment
As shown in Figure 1-3,
The present invention, a kind of intelligent immunity cell culture oscillator, including power device, concussion plate 1, mounting platform and control
Device,
The power device includes motor, crankshaft 2 and buffer 3, and the motor driven crankshafts 2 move in a circle, crankshaft 2
Buffer 3 is driven by connecting rod 4, buffer 3 is fixed on concussion plate 1；
Buffer 3 include dashpot 31 closed at both ends and and the sliding block 32 that is moved back and forth in dashpot 31, the cunning
Block, 32 are connected with connecting rod 4, and the dashpot 31 is connected with concussion plate 1, and the both ends of dashpot 31 are equipped with buffer spring 33.
1 upper surface of concussion plate is equipped with no less than 2 test tube fixed frames 11, and concussion 1 lower surface of plate is equipped with directive wheel 12
With stopper tab 13,13 two sides of stopper tab are equipped with braking silica gel 5, and braking silica gel 5 is fixed on mounting platform, and the installation is flat
The track 6 matched with directive wheel 12 is additionally provided on platform；
The motor is electrically connected with the controller.
The test tube fixed frame 11 is equipped with test tube jack, is equipped with laser detector, the laser detection at test tube jack
Device includes the laser beam emitting device 111 and laser receiver 112 positioned at test tube jack two sides, 111 He of laser beam emitting device
Laser receiver 112 is electrically connected with the controller.
Each test tube jack is on test tube receptacle axis direction is equipped with no less than 3 groups of laser detectors in parallel.
The top and bottom of the test tube jack are respectively equipped with silicone gasket 113.
One embodiment of the present invention has been described in detail above, but the content is only preferable implementation of the invention
Example, should not be considered as limiting the scope of the invention.It is all according to all the changes and improvements made by the present patent application range
Deng should still be within the scope of the patent of the present invention.
Claims (5)
1. a kind of intelligent immunity cell culture oscillator, which is characterized in that including power device, concussion plate, mounting platform and control
Device processed,
The power device includes motor, crankshaft and buffer, and the motor driven crankshafts move in a circle, and crankshaft passes through connecting rod
Buffer is driven, buffer is fixed on concussion plate；
The concussion plate upper surface is equipped with no less than 2 test tube fixed frames, and concussion plate lower surface is equipped with directive wheel and stopper tab,
Stopper tab two sides are equipped with braking silica gel, and braking silica gel solid is additionally provided with and is oriented on the mounting platform due on mounting platform
Take turns the track matched；
The motor is electrically connected with the controller.
2. intelligent immunity cell culture oscillator according to claim 1, it is characterised in that: the test tube fixed frame is equipped with
Test tube jack, laser detector is equipped at test tube jack, and the laser detector includes swashing positioned at test tube jack two sides
Light emitting devices and laser receiver, laser beam emitting device and laser receiver are electrically connected with the controller.
3. intelligent immunity cell culture oscillator according to claim 2, it is characterised in that: each test tube jack is in examination
No less than 3 groups of laser detectors are equipped on pipe receptacle axis direction in parallel.
4. intelligent immunity cell culture oscillator according to claim 3, it is characterised in that: the upper end of the test tube jack
Silicone gasket is respectively equipped with lower end.
5. intelligent immunity cell culture oscillator according to claim 1, it is characterised in that: buffer includes closed at both ends
Dashpot and be connected with the sliding block that is moved back and forth in dashpot, the sliding block with connecting rod, the dashpot and concussion plate phase
Even, the both ends of dashpot are equipped with buffer spring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810980295.1A CN108949561A (en) | 2018-08-27 | 2018-08-27 | Intelligent immunity cell culture oscillator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810980295.1A CN108949561A (en) | 2018-08-27 | 2018-08-27 | Intelligent immunity cell culture oscillator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108949561A true CN108949561A (en) | 2018-12-07 |
Family
ID=64474480
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810980295.1A Pending CN108949561A (en) | 2018-08-27 | 2018-08-27 | Intelligent immunity cell culture oscillator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108949561A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112337372A (en) * | 2020-10-21 | 2021-02-09 | 南京秉蓬生物科技有限公司 | Shaking mixing device for cell culture and use method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202786253U (en) * | 2012-08-22 | 2013-03-13 | 索微生物技术（苏州）有限公司 | Automatic blood culture instrument |
| CN104014270A (en) * | 2014-06-28 | 2014-09-03 | 葛华鹏 | Vibrating type test tube shaking device |
| CN204380599U (en) * | 2015-01-14 | 2015-06-10 | 强怒萍 | A kind of vibrating type blood test-tube oscillating uniform device |
| WO2017107837A1 (en) * | 2015-12-23 | 2017-06-29 | 上海吉凯基因科技有限公司 | Fully automated continuous cell culture system |
| CN107570072A (en) * | 2017-10-27 | 2018-01-12 | 孙宇飞 | A kind of chemistry uses speed governing rocking equipment |
-
2018
- 2018-08-27 CN CN201810980295.1A patent/CN108949561A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202786253U (en) * | 2012-08-22 | 2013-03-13 | 索微生物技术（苏州）有限公司 | Automatic blood culture instrument |
| CN104014270A (en) * | 2014-06-28 | 2014-09-03 | 葛华鹏 | Vibrating type test tube shaking device |
| CN204380599U (en) * | 2015-01-14 | 2015-06-10 | 强怒萍 | A kind of vibrating type blood test-tube oscillating uniform device |
| WO2017107837A1 (en) * | 2015-12-23 | 2017-06-29 | 上海吉凯基因科技有限公司 | Fully automated continuous cell culture system |
| CN107570072A (en) * | 2017-10-27 | 2018-01-12 | 孙宇飞 | A kind of chemistry uses speed governing rocking equipment |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112337372A (en) * | 2020-10-21 | 2021-02-09 | 南京秉蓬生物科技有限公司 | Shaking mixing device for cell culture and use method |
| CN112337372B (en) * | 2020-10-21 | 2022-04-12 | 南京秉蓬生物科技有限公司 | Shaking mixing device for cell culture and use method |
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| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
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| RJ01 | Rejection of invention patent application after publication | ||
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Application publication date: 20181207 |