CN108949499A - A kind of rotary reagent extraction amplification device - Google Patents
A kind of rotary reagent extraction amplification device Download PDFInfo
- Publication number
- CN108949499A CN108949499A CN201810606066.3A CN201810606066A CN108949499A CN 108949499 A CN108949499 A CN 108949499A CN 201810606066 A CN201810606066 A CN 201810606066A CN 108949499 A CN108949499 A CN 108949499A
- Authority
- CN
- China
- Prior art keywords
- slot
- reagent
- elution
- oil groove
- amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000003321 amplification Effects 0.000 title claims abstract description 77
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 77
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 46
- 238000000605 extraction Methods 0.000 title claims abstract description 15
- 230000007246 mechanism Effects 0.000 claims abstract description 56
- 238000010828 elution Methods 0.000 claims abstract description 41
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 36
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 36
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 36
- 238000005192 partition Methods 0.000 claims abstract description 29
- 239000000463 material Substances 0.000 claims abstract description 27
- 238000005336 cracking Methods 0.000 claims abstract description 24
- 239000000284 extract Substances 0.000 claims abstract description 22
- 238000005406 washing Methods 0.000 claims abstract description 11
- 239000003480 eluent Substances 0.000 claims abstract description 7
- 230000002101 lytic effect Effects 0.000 claims abstract description 7
- 238000004140 cleaning Methods 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 44
- 230000003139 buffering effect Effects 0.000 claims description 24
- 238000007789 sealing Methods 0.000 claims description 24
- 239000011324 bead Substances 0.000 claims description 7
- 238000011144 upstream manufacturing Methods 0.000 claims description 5
- 239000012744 reinforcing agent Substances 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 17
- 230000003416 augmentation Effects 0.000 abstract description 3
- 238000006243 chemical reaction Methods 0.000 description 9
- 238000000034 method Methods 0.000 description 9
- 230000008569 process Effects 0.000 description 7
- 238000012864 cross contamination Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 238000012408 PCR amplification Methods 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- 238000003556 assay Methods 0.000 description 3
- 230000005611 electricity Effects 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 230000005389 magnetism Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003190 augmentative effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Abstract
The present invention provides a kind of rotary reagents to extract amplification device comprising: extract storehouse comprising cracking mechanism, washing mechanism and the elution mechanism being sequentially distributed according to sample flow direction；It cracks and is equipped with lytic reagent and magnetisable material in mechanism；Washing mechanism includes the first oil groove and sink, is equipped with cleaning solution in sink；First oil groove is located between cracking mechanism and sink；Eluting mechanism includes the second oil groove and elution slot, is equipped with eluent in elution slot；Second oil groove is located between sink and elution slot；Oily phase is equipped in first oil groove and the second oil groove；Mobilizable partition is equipped between adjacent two slot；It cracks and is equipped with mobilizable partition between mechanism and the first oil groove；Driving mechanism comprising the sliding block of sliding can be flowed to according to sample；Sliding block is equipped with magnet；Amplification pipe is located at elution mechanism downstream；Amplification pipe is connected to elution slot.The present invention can rotatably integrate the extraction of nucleic acid and augmentation detection under totally-enclosed environment, high-efficient simple, as a result accurately.
Description
Technical field
The present invention relates to a kind of rotary reagents to extract amplification device.
Background technique
Nucleic acid diagnostic assay method based on round pcr is the core technology in molecular diagnosis.Nucleic acid is mentioned automatically
It takes process and PCR amplification detection process mutually integrated, integrated nucleic acid extraction and augmentation detection system is constructed, for further
Nucleic acid diagnostic assay efficiency is improved, realizes that the automation of entire nucleic acid diagnostic assay process has important practical significance.
Chinese patent 201120449250.5 discloses a kind of nucleic acid amplification and detection reaction tube, including mutual cooperation tube body
With pipe lid, tube body includes the liquid storage area being located above and underlying nucleic acid amplification area, completes to react in nucleic acid amplification area
And the real-time detections means such as fluorescence signal acquisition；Medium altitude groove is equipped with inside pipe lid, and the volume in nucleic acid amplification area is respectively smaller than
Or equal to the volume of liquid storage area and the volume of medium altitude groove.
Chinese patent 201521043284.9 discloses a kind of isothermal nucleic acid amplification device and experimental method, including plate is former
Position nucleic acid amplification chip, the chip is by being bonded to each other, slidable upper and lower layer carrier is constituted, and upper and lower layer carrier is same size
Rectangle, when lower layer is fixed, upper layer carrier can relative friction sliding.Various open is opened up on the upper binding face of lower layer's carrier
The downward groove runner of mouth, microcavity, open up groove discharge orifice, the microcavity of various opening upwards on the lower binding face of upper layer carrier,
And some import and export through-holes.
Chinese patent 201620284715.9 discloses a kind of multi-functional nucleic acid augmentative instrument, including control system, switch electricity
Source, host computer, heating circulation system and sample module, control system connection switch power supply, host computer and heating circulation system, add
The top of heat circulating system connects sample module, 384 orifice plates of the sample module equipped with 0.2mL, 96 orifice plates, 48 orifice plates and
12 orifice plates of 1.5mL.
Chinese patent application 201710163203.6 discloses integrated nucleic acid extraction and augmentation detection system, the system packet
Include disposable micro-fluidic chip and mating detection device.Wherein micro-fluidic chip includes reaction member and waste liquid storage unit, core
When piece works, being mutually mixed between reaction reagent, between reaction reagent and magnetic bead is realized by vibration module, is driven by magnetic bead
Dynamic model block, be sequentially completed nucleic acid absorption, purifying and etc.；PCR amplification reagent is allowed to rinse magnetic bead, elution by fluid drive module
Amplification template on magnetic bead passes through heating module and fluorescent collecting mould into the convection current PCR reactor constructed by vertical channel
Block realizes pcr amplification reaction and detection.
The defect of above-mentioned technical proposal is:
1, structure is complicated, needs vacuum pump；And lysate is more sticky, vacuum pump operation is easy to produce bubble, influences to mention
It takes；
2, waste liquid can pass through nucleic acid amplification test chamber, there is waste liquid residual contamination, influence nucleic acid amplification detection；
Both 3, detection process is not closed, and the environment outside detection device is caused to contact with the reagent in detection device, cause
There are the risks of cross contamination；
4, detection process is cumbersome, and multiple links need to increase manual operations；
5, detection time is long.
Therefore, how to provide a kind of without vacuum pump, instrument and equipment structure is simpler；Bubble is not generated, is conducive to extract
It goes on smoothly, obtains the higher nucleic acid of purity；With individual nucleic acid amplification test chamber, there is no waste liquid remaining influence；It detected
Journey closing, the reagent extraction amplification device that can effectively avoid external environment and detection reagent cross contamination become industry and need to solve
Certainly the problem of.
Summary of the invention
In view of the shortcomings of the prior art, the object of the present invention is to provide a kind of rotary reagents to extract amplification device, knot
Structure is simple, efficiently and accurately, and detection process closing can effectively avoid external environment and detection reagent cross contamination.
To achieve the goals above, the present invention provides a kind of rotary reagents to extract amplification device, and reagent extracts amplification
Device includes:
Storehouse is extracted, extracting storehouse includes the cracking mechanism being sequentially distributed according to sample flow direction, washing mechanism and elution mechanism；It splits
It solves and is equipped with lytic reagent and magnetisable material in mechanism；Washing mechanism includes the first oil groove and sink, is equipped with washing in sink
Liquid；First oil groove is located between cracking mechanism and sink；Eluting mechanism includes the second oil groove and elution slot, elutes and is equipped in slot
Eluent；Second oil groove is located between sink and elution slot；Oily phase is equipped in first oil groove and the second oil groove；Adjacent two slot
Between be equipped with mobilizable partition；It cracks and is equipped with mobilizable partition between mechanism and the first oil groove；Partition is rotatable close
Seal axis；
Driving mechanism, driving mechanism include the sliding block that sliding can be flowed to according to sample；Sliding block is equipped with magnet；
Amplification pipe, amplification pipe are located at elution mechanism downstream；Amplification pipe is connected to elution slot.
In the present invention, extract in storehouse, for the quantity of slot depending on needed for carried reagent or solid matter, capacity can be not to the utmost
It is identical.
In the present invention, driving mechanism further comprises the power part for driving sliding block to run, and power part can be electricity
Machine or cylinder.
In the present invention, the nucleic acid generated after sample cracking is attached on magnetisable material；When slide block movement, driven by magnet
Magnetisable material movement, to drive nucleic acid movement.
In the present invention, each slot is along lineal layout；In use, extract storehouse can be horizontally arranged, vertically place or
Slant setting.
In the present invention, when sliding block is by separating, driving mechanism can make partition fail, and the slot for separating two sides communicates；Part
Separate permanent failure, partial partition restores once again after sliding block passes through.
The present invention solve nucleic acid extraction and nucleic acid amplification detection cannot rotary, nucleic acid extraction and nucleic acid amplification detected
The technical issues of journey trivial operations, high-efficient simple, as a result accurately.
Another specific embodiment according to the present invention, cracking mechanism include according to sample flow direction be sequentially distributed magnetic slot,
Splitter and engagement groove；It cracks in mechanism, mobilizable partition is equipped between adjacent two slot；It is set between engagement groove and the first oil groove
There is mobilizable partition.
Another specific embodiment, cracking mechanism further comprise the sample positioned at magnetic slot upstream to sample lid according to the present invention
This slot, sample are located in sample slot；Lytic reagent includes that cracking reinforcing agent and cracking combine liquid, is respectively arranged on splitter and combination
In slot；Magnetisable material is set in magnetic slot.
In the present solution, sample slot is equipped with the feed inlet for adding sample, feed inlet is equipped with sample lid, is used for sample
Add rear enclosed sample slot.
Another specific embodiment according to the present invention, extracting storehouse further comprises buffering liquid groove, and buffering liquid groove is located at elution
Slot downstream, and mobilizable partition is equipped between elution slot；Buffering liquid groove is located at amplification pipe upstream, and communicates with amplification pipe.
In the present solution, buffering liquid groove is connected with amplification pipe by connector, connector is equipped with discharge port；Sample is by going out
Material mouth flows into amplification pipe from buffering liquid groove.
In addition it is also possible to not set amplification pipe, and carried out amplification reaction in buffering liquid groove；At this point, no setting is required connector,
Discharge port is directly set on buffering liquid groove, the outflow for sample after amplified reaction.
Another specific embodiment according to the present invention is equipped with rotatable sealing axis between adjacent two slot, seals and sets on axis
There is channel.
Another specific embodiment according to the present invention is equipped with the seal groove with sealing axis cooperation, sealing between adjacent two slot
Slot is correspondingly arranged with sealing axis.
Another specific embodiment according to the present invention, magnet are located at center, are permanent magnet or electromagnet.
Another specific embodiment according to the present invention, magnetisable material are magnetic bead.
Another specific embodiment according to the present invention, it further comprises top cover that reagent, which extracts amplification device, and top cover is detachable
Silo roof end is extracted in ground connection, and is closed and extracted each slot in storehouse.
In the present solution, the size and shape for extracting each slot in storehouse is on-demand customized, quantity is depending on reaction requirement.
It seals axis and is equipped with knob, knob is located above channel；Top cover is equipped with several rotating holes, and axis is corresponding sets with sealing
It sets；Knob passes through corresponding rotating hole.
Top cover further comprises two piston holes, is located above elution slot and buffering liquid groove；Each piston hole is equal
There is a piston to pass through.
Another specific embodiment according to the present invention, amplification pipe is interior to be equipped with the amplification examination expanded for realizing nucleic acid increment
Agent, amplifing reagent are PCR platform reagent or constant-temperature amplification reagent.
Operating principle of the invention is following (magnet is electromagnet):
1, sample slot is added in sample, closes feed inlet；Meanwhile sliding block is placed in below sample slot；At this point, magnet is not yet logical
Electricity does not have magnetism；
2, sliding block is mobile to amplification pipe direction, and partition failure, liquid flows into magnetic slot；
3, sliding block continues to move to, and partition failure, liquid flows into splitter；
4, sliding block continues to move to, and partition failure, liquid flows into engagement groove；
5, magnetisable material is uniformly diffused into cracking and combines in liquid；Sample is cleaved, and discharges nucleic acid；Nucleic acid is with magnetisable material knot
It is combined；
6, magnet is powered, and starts have magnetism；Sliding block continues to move to, and partition failure, magnetisable material flows into the first oil groove；
7, sliding block continues to move to, and separates temporarily failure, and magnetisable material flows into sink；When sliding block is located at below sink
When, the partition between the first oil groove and sink restores, and continues to separate the first oil groove and sink；
8, magnet powers off, and magnetisable material is uniformly diffused into cleaning solution；Impurity on magnetisable material is washed off, and is transferred to
In cleaning solution；
9, magnet is powered, and sliding block continues to move to, and partition failure, magnetisable material flows into the second oil groove；
10, sliding block continues to move to, and separates temporarily failure, and magnetisable material flows into elution slot；When sliding block is located at below elution slot
When, the partition between the second oil groove and elution slot restores, and continues to separate the second oil groove and elution slot；
11, magnet powers off, and magnetisable material is uniformly diffused into eluent；Nucleic acid on magnetisable material is washed off, and is shifted
Into eluent；
12, magnet is powered, and sliding block continues to move to, and partition failure, liquid flows into buffering liquid groove；Sliding block back moves, and is parked in
It elutes below slot, is retained in magnetisable material in elution slot；Nucleic acid stays in buffering liquid groove；
13, in the case where the external world squeezes, the liquid in buffering liquid groove flows into amplification pipe, and carries out nucleic acid amplification in amplification is managed.
Compared with prior art, the present invention have it is following the utility model has the advantages that
Nucleic acid extraction and nucleic acid amplification can be detected seamless union by the present invention, it is only necessary to sample be added, can be obtained in 1 hour
As a result；Whole process carries out under totally-enclosed environment without being further added by operating procedure, can effectively avoid external environment and inspection
Test agent cross contamination；Lysate, cleaning solution, eluent are separately fixed at individual liquid storage cavity, and with oily mutually by liquid
It separates two-by-two, mutually will not directly be contacted between liquid, avoid cross contamination；Amplification pipe be also be separately provided, be more advantageous into
Row amplified reaction.
The present invention is described in further detail with reference to the accompanying drawing.
Detailed description of the invention
Fig. 1 is that the reagent of embodiment 1 extracts the explosive view of amplification device；
Fig. 2 is that the reagent of embodiment 1 extracts the cross-sectional view of amplification device；
Fig. 3 is the structural schematic diagram in the extraction storehouse of embodiment 1；
Fig. 4 is the structural schematic diagram of the top cover of embodiment 1；
Fig. 5 is the structural schematic diagram of the sliding block of embodiment 1；
Fig. 6 is the structural schematic diagram of the sealing axis of embodiment 1；
Fig. 7 is that the reagent of embodiment 1 extracts the overall structure diagram of amplification device.
Specific embodiment
Embodiment 1
A kind of rotary reagent extraction amplification device is present embodiments provided, as shown in figs. 1-7 comprising extraction storehouse 21,
Crack mechanism 22, washing mechanism 23, elution mechanism 24, driving mechanism 25, amplification pipe 26 and top cover 27.
Wherein, extracting storehouse 21 includes the cracking mechanism 22 being sequentially distributed according to sample flow direction, washing mechanism 23, elution mechanism
24 and buffering liquid groove 2101.
It cracks and is equipped with lytic reagent and magnetisable material in mechanism 22；Magnetisable material is magnetic bead.
Washing mechanism 23 includes the first oil groove 2301 and sink 2302, is equipped with cleaning solution in sink 2302；First oil
Slot 2301 is located between cracking mechanism 22 and sink 2302.
Eluting mechanism 24 includes the second oil groove 2401 and elution slot 2402, is equipped with eluent in elution slot 2402；Second oil
Slot 2401 is located between sink 2302 and elution slot 2402；Oily phase is equipped in first oil groove 2301 and the second oil groove 2401；
Mobilizable partition is equipped between adjacent two slot；It cracks and is equipped with mobilizable partition between mechanism 22 and the first oil groove 2301.
Cracking mechanism 22 includes the sample slot 2201 being sequentially distributed according to sample flow direction, magnetic slot 2202, splitter 2203
With engagement groove 2204；It cracks in mechanism 22, mobilizable partition is equipped between adjacent two slot；Engagement groove 2204 and the first oil groove
Mobilizable partition is equipped between 2301；Sample slot 2201 is equipped with the feed inlet 2205 for adding sample, feed inlet 2205
It is equipped with sample lid 2206, adds rear enclosed sample slot 2201 for sample.
Lytic reagent includes that cracking reinforcing agent and cracking combine liquid, is respectively arranged in splitter 2203 and engagement groove 2204；
Magnetisable material is set in magnetic slot 2202.
Driving mechanism 25 includes the sliding block 2501 of sliding being flowed to according to sample and for driving the dynamic of the operation of sliding block 2501
Power component (not shown), power part are motor；Sliding block 2501 is equipped with magnet 2502；Magnet 2502 is located in sliding block 2501
The heart is electromagnet.
It expands pipe 26 and is located at elution 24 downstream of mechanism；Amplification pipe 26 is connected to elution slot 2402；It is equipped with and is used in amplification pipe
Realize that the amplifing reagent of nucleic acid increment amplification, amplifing reagent are constant-temperature amplification reagent.
Buffering liquid groove 2101 is located at elution 2402 downstream of slot, and is equipped with mobilizable partition between elution slot 2402；It is slow
Fliud flushing slot 2101 is located at amplification 26 upstream of pipe, and communicates with amplification pipe 26；Buffering liquid groove 2101 and amplification pipe 26 pass through connector
2102 are connected, and connector 2102 is equipped with discharge port 2103；Sample is flowed into from buffering liquid groove 2101 by discharge port 2103 and is expanded
Pipe 26.
In the present embodiment, rotatable sealing axis 28 is equipped between adjacent two slot, sealing axis 28 is equipped with 2801 He of channel
Knob 2802, knob 2802 are located at 2801 top of channel；Detachably connected 21 top of extraction storehouse of top cover 27, and close and extract storehouse
21 each slots；The seal groove 29 cooperated with sealing axis 28 is equipped between adjacent two slot, seal groove 29 is correspondingly arranged with sealing axis 28.
Top cover 27 is equipped with several rotating holes 2701 and two piston holes 2702, is correspondingly arranged with sealing axis 28；Knob
2802 pass through corresponding rotating hole 2701；Two piston holes 2702 are located at 2101 top of elution slot 2402 and buffering liquid groove；
Each piston hole 2702 has a piston 2703 to pass through.
The application method of the present embodiment is as follows:
1, sample lid 2206 is removed, sample is added, covers sample lid 2206；At this point, between channel 2801 and its two side channel
Line it is vertical, prevent the substance circulating in two slots, play the role of isolation；
2, under power part driving, sliding block 2501 is mobile toward amplification 26 direction of pipe；When sliding block 2501 is by sealing axis 28
When, sealing axis 28 is rotated by 90 °, and adjacent two slot is connected to by channel 2801；
3, sample flows into magnetic slot 2202 through channel 2801, and magnetisable material carries sample information；Under the effect of magnet 2502,
The magnetisable material for carrying sample information is continued to move to sliding block 2501；When by sealing axis 28, step 2 is repeated；First oil groove
Sealing axis 28 between sealing axis 28 and the second oil groove 2401 between 2301 and sink 2302 and elution slot 2402 is in cunning
After block 2501 passes through, it is rotated by 90 °, recloses two side channels；Remaining sealing axis 28 no longer rotates；
4, when magnetisable material is retained in elution slot 2402, and nucleic acid is stayed in buffering liquid groove 2101, work is pushed down on
Plug 2703 squeezes buffering liquid groove 2101；Liquid in buffering liquid groove 2101 flows into amplification pipe 26, and carries out in amplification pipe 26
PCR amplification.
Embodiment 2
The present embodiment the difference from embodiment 1 is that: extract storehouse place vertically.
Embodiment 3
The present embodiment the difference from embodiment 1 is that: buffering liquid groove and amplification pipe between be equipped with third oil groove, third oil groove
It is interior to be equipped with oily phase；It is equipped with rotatable sealing axis between buffering liquid groove and third oil groove, is equipped between amplification pipe and third oil groove
Rotatable sealing axis.
Although the present invention is disclosed above in the preferred embodiment, it is not intended to limit the invention the range of implementation.Any
The those of ordinary skill in field is not departing from invention scope of the invention, improves when can make a little, i.e., all according to this hair
Bright done same improvement, should be the scope of the present invention and is covered.
Claims (10)
1. a kind of rotary reagent extracts amplification device, wherein the reagent extracts amplification device and includes:
Storehouse is extracted, the storehouse of extracting includes the cracking mechanism being sequentially distributed according to sample flow direction, washing mechanism and elution mechanism；Institute
It states in cracking mechanism and is equipped with lytic reagent and magnetisable material；The washing mechanism includes the first oil groove and sink, the washing
Cleaning solution is equipped in slot；First oil groove is between the cracking mechanism and the sink；The elution mechanism includes
Second oil groove and elution slot, the elution slot is interior to be equipped with eluent；Second oil groove is located at the sink and the elution
Between slot；Oily phase is equipped in first oil groove and second oil groove；Mobilizable partition is equipped between adjacent two slot；Institute
It states and is equipped with mobilizable partition between cracking mechanism and first oil groove；The partition is rotatable sealing axis；
Driving mechanism, the driving mechanism include the sliding block that sliding can be flowed to according to sample；The sliding block is equipped with magnet；
Amplification pipe, the amplification pipe are located at the elution mechanism downstream；The amplification pipe is connected to the elution slot.
2. reagent as described in claim 1 extracts amplification device, wherein the cracking mechanism includes flowing to successively according to sample
Magnetic slot, splitter and the engagement groove of distribution；In the cracking mechanism, mobilizable partition is equipped between adjacent two slot；It is described
Mobilizable partition is equipped between engagement groove and first oil groove.
3. reagent as claimed in claim 2 extracts amplification device, wherein the cracking mechanism further comprises being located at the magnetic
The sample slot of property slot upstream, sample are located in the sample slot；The lytic reagent includes that cracking reinforcing agent and cracking combine liquid,
It is respectively arranged in the splitter and the engagement groove；The magnetisable material is set in the magnetic slot.
4. reagent as claimed in claim 3 extracts amplification device, wherein the extraction storehouse further comprises buffering liquid groove, institute
It states buffering liquid groove and is located at the elution slot downstream, and be equipped with mobilizable partition between the elution slot；The buffering liquid groove
It is communicated positioned at the amplification pipe upstream, and with the amplification pipe.
5. reagent as claimed in claim 4 extracts amplification device, wherein rotatable sealing axis is equipped between adjacent two slot,
The sealing axis is equipped with channel.
6. reagent as claimed in claim 5 extracts amplification device, wherein be equipped between adjacent two slot and cooperate with the sealing axis
Seal groove, the seal groove is correspondingly arranged with the sealing axis.
7. reagent as described in claim 1 extracts amplification device, wherein the magnet is located at the center, for forever
Magnet or electromagnet.
8. reagent as described in claim 1 extracts amplification device, wherein the magnetisable material is magnetic bead.
9. reagent as claimed in claim 6 extracts amplification device, wherein it further comprises top that the reagent, which extracts amplification device,
Lid, the detachably connected extraction silo roof end of top cover.
10. reagent as described in claim 1 extracts amplification device, wherein be equipped in the amplification pipe for realizing nucleic acid increasing
Measure the amplifing reagent of amplification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810606066.3A CN108949499A (en) | 2018-06-12 | 2018-06-12 | A kind of rotary reagent extraction amplification device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810606066.3A CN108949499A (en) | 2018-06-12 | 2018-06-12 | A kind of rotary reagent extraction amplification device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108949499A true CN108949499A (en) | 2018-12-07 |
Family
ID=64488845
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810606066.3A Pending CN108949499A (en) | 2018-06-12 | 2018-06-12 | A kind of rotary reagent extraction amplification device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108949499A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111321056A (en) * | 2020-02-27 | 2020-06-23 | 浙江大学 | Cartridge device and method for nucleic acid extraction and analysis |
| CN114574324A (en) * | 2022-04-26 | 2022-06-03 | 广州国家实验室 | Sample extraction device |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040161788A1 (en) * | 2003-02-05 | 2004-08-19 | Shuqi Chen | Sample processing |
| CN101149376A (en) * | 2006-09-19 | 2008-03-26 | 财团法人工业技术研究院 | Magnetic bead type inspected body separation device |
| CN103897016A (en) * | 2012-12-26 | 2014-07-02 | 深圳华大基因研究院 | Magnetic bead-based treatment device and treatment method using the same |
| CN104762193A (en) * | 2015-04-21 | 2015-07-08 | 北京化工大学 | Microfluidic device for automatic extraction of nucleic acid |
| CN105562132A (en) * | 2016-01-04 | 2016-05-11 | 上海医脉赛科技有限公司 | Device for extracting and detecting biological sample |
| CN107541462A (en) * | 2017-09-13 | 2018-01-05 | 北京理工大学 | One kind is used for the system and application process of nucleic acid purification, amplification and genetic test |
-
2018
- 2018-06-12 CN CN201810606066.3A patent/CN108949499A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040161788A1 (en) * | 2003-02-05 | 2004-08-19 | Shuqi Chen | Sample processing |
| CN101149376A (en) * | 2006-09-19 | 2008-03-26 | 财团法人工业技术研究院 | Magnetic bead type inspected body separation device |
| CN103897016A (en) * | 2012-12-26 | 2014-07-02 | 深圳华大基因研究院 | Magnetic bead-based treatment device and treatment method using the same |
| CN104762193A (en) * | 2015-04-21 | 2015-07-08 | 北京化工大学 | Microfluidic device for automatic extraction of nucleic acid |
| CN105562132A (en) * | 2016-01-04 | 2016-05-11 | 上海医脉赛科技有限公司 | Device for extracting and detecting biological sample |
| CN107541462A (en) * | 2017-09-13 | 2018-01-05 | 北京理工大学 | One kind is used for the system and application process of nucleic acid purification, amplification and genetic test |
Non-Patent Citations (2)
| Title |
|---|
| 王念跃;马超;李传燕;王芳;: "磁性纳米颗粒Fe_3O_4@SiO_2的制备及在全血DNA提取中的应用", vol. 34, no. 03, pages 169 - 172 * |
| 郑旭煦: "水暖工操作技术要领图解", vol. 2, 武汉：华中科技大学出版社, pages: 90 - 92 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111321056A (en) * | 2020-02-27 | 2020-06-23 | 浙江大学 | Cartridge device and method for nucleic acid extraction and analysis |
| CN111321056B (en) * | 2020-02-27 | 2023-11-21 | 浙江大学 | Cartridge device and method for nucleic acid extraction and analysis |
| CN114574324A (en) * | 2022-04-26 | 2022-06-03 | 广州国家实验室 | Sample extraction device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110452802A (en) | It is complete to extract molecular diagnosis micro-fluidic chip and microfluidic system | |
| CN104487562B (en) | Biochemical treatment apparatus | |
| CN107398307A (en) | A kind of integrated micro-flow control chip | |
| CN108642148A (en) | A kind of nucleic acid amplification detection micro-fluidic chip and its detection method | |
| CN103454439B (en) | A kind of micro-fluidic chip integral type sampling system | |
| CN105349401A (en) | Multifunctional integrated microfluidic nucleic acid analysis chip and preparation and analysis method thereof | |
| CN206375900U (en) | A kind of miniflow bag for nucleic acid integration detection | |
| CN207933420U (en) | A kind of micro-fluidic chip of genetic test | |
| CN206375901U (en) | A kind of integrated closed microfluidic nucleic acid detection means | |
| CN110308295A (en) | A kind of micro-fluidic multichannel sample introduction and cleaning device and cleaning method with it | |
| CN105879936A (en) | Whole blood filtration and quantitative transfer micro-fluidic chip | |
| CN109603936B (en) | Elastic micro-fluidic chip for tuberculosis detection | |
| CN111909835A (en) | Closed micro-fluidic nucleic acid detection card box | |
| CN109289949A (en) | A kind of full-automatic microlayer model emulsion generating means and its generation method | |
| CN107723210A (en) | Novel nucleic acids detect micro flow control chip device | |
| CN205517819U (en) | Whole blood filtration and ration are moved and are got micro -fluidic chip | |
| CN108753968B (en) | Kit for detecting methylation of cervical cancer PAX1 gene | |
| CN108949499A (en) | A kind of rotary reagent extraction amplification device | |
| CN110193387A (en) | A kind of totally-enclosed micro-fluidic chip and lotion droplet preparation system | |
| WO2020107641A1 (en) | Biological reaction device provided with microfluidic or nanofluidic structure | |
| CN108949498A (en) | A kind of Full enclosed integrated reagent extraction amplification device | |
| CN108913530A (en) | A kind of Full enclosed integrated reagent extraction amplification method | |
| CN209254790U (en) | A kind of full-automatic microlayer model emulsion generating means | |
| CN209481641U (en) | A kind of anti-pollution gene assaying device | |
| CN108004129A (en) | A kind of nucleic acid integration Multiple detection box body and detection method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |