CN206375901U - A kind of integrated closed microfluidic nucleic acid detection means - Google Patents
A kind of integrated closed microfluidic nucleic acid detection means Download PDFInfo
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- CN206375901U CN206375901U CN201621377472.XU CN201621377472U CN206375901U CN 206375901 U CN206375901 U CN 206375901U CN 201621377472 U CN201621377472 U CN 201621377472U CN 206375901 U CN206375901 U CN 206375901U
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Abstract
The utility model is related to a kind of integrated closed microfluidic nucleic acid detection means, main to include one piece of substrate plate, and substrate plate is provided with multistage runner, and the pre-packaged chamber for having a reagent.Pass through unique flow passage between its core hybrid reaction chamber and other chambers.The runner needed for being gated using outside breach ring-type instrument is only needed during detection of nucleic acids, you can the order of control reagent reacting.Device is provided with bar code, scans bar code, and the parameter of detection of nucleic acids, such as sample type, temperature, time etc. are then obtained on software kit.Closing can be realized using the utility model, integration is inexpensive, easily detection of nucleic acids.
Description
Technical field
The utility model is related to diagnostic technique in molecular biology field, more particularly to a kind of integrated closed microfluidic nucleic acid inspection
Survey device.
Background technology
Detection of nucleic acids suffers from very heavy in many biochemical analysis fields such as clinical medicine, forensic identification, heredity inspection etc.
Big effect, has been widely used in the fields such as biological medicine.Detection of nucleic acids means used in present every field are still
Old is traditional method, i.e., manually extract the nucleic acid in sample first, then good to extraction purification in commercial PCR instrument
Nucleic acid expanded or simultaneously detect (real-time fluorescence PCR), or PCR terminate after by detected through gel electrophoresis amplified production.It is whole
Individual detection process operation is comparatively laborious, disperses, is also easy to produce cross pollution.If therefore by the closed reality of detection of nucleic acids integrated form
It is existing, cross contamination risk can be greatly reduced, the accuracy and security of detection is improved.
The fast development of microflow control technique is provided convenience for nucleic acid detection technique in recent years, and microflow control technique can be by routine
Operating process miniaturization and scale.But it is integrated with the micro-valves of many in most of microfluidic chip devices, the small member such as Micropump
Part controls the flowing of liquid, and it processes smart small, complexity, and peripheral control system is also excessively complicated, this to detect every time into
This is too high, is not suitable for substantial amounts of experimental study.
The utility model is directed to above present situation, a kind of integrated closed microfluidic nucleic acid detection means of design, it is to avoid sample
Pollution in extraction and amplification procedure, effectively raises the convenience and accuracy of detection of nucleic acids.
Utility model content
The utility model purpose is to provide a kind of integrated closed microfluidic nucleic acid detection means, and laboratory technician only need to will treat sample
Originally the relevant position in device is added to, then only empirically flow operations can accomplished detection of nucleic acids to the present apparatus.
The technical solution of the utility model is：A kind of integrated closed microfluidic nucleic acid detection means, it is main to include one piece of base
Bottom plate, substrate plate is provided with multistage runner, and the pre-packaged chamber for having a reagent.
All reagents needed for detection of nucleic acids, the cracking of nucleic acid extraction part are contained in chamber on described substrate plate
Liquid, magnetic nanoparticle, with reference to liquid, multiple kinds of cleaning agent, eluent, augmentation detection reaction solution etc..Core chamber on substrate plate
It is hybrid reaction chamber, other chambers are distributed in around mixture reaction chamber, between each chamber and hybrid reaction chamber
There is flow passage.When carrying out detection of nucleic acids, only need outside by a simple breach ring-type instrument switching chamber indoor liquid
Flow into the order of hybrid reaction chamber.Chamber on substrate plate is flexible, can be by pressing chamber so that liquid in chamber
Outflow, without on substrate plate integrated multiple valve pumps control the flowing of liquid.Due to the characteristic distributions of each chamber so that multiple
It is that can also reduce substrate plate upper chamber as waste chamber in reagent storage chamber, course of reaction before chamber detection of nucleic acids
Number so that whole device is more compact.
Augmentation detection chamber in described chamber is provided with multiple, the Multiple detection available for nucleic acid.
The substrate plate is provided with a well, and sample to be detected flows into runner by well, and sample passes through runner stream
Enter lysate reaction chamber.The well outside can be sealed after injecting sample, prevent liquid from being overflowed from well.
Bar code is additionally provided with described substrate plate, bar code scanning is carried out before carrying out detection of nucleic acids, can be corresponding
The automatic parameter set needed for experiment, such as temperature, time etc. in control software.
The material of described substrate plate can be dimethyl silicone polymer (PDMS), polymethyl methacrylate (PMMA),
Makrolon (PC) or other polymeric materials.
When carrying out detection of nucleic acids, first sample is added in device by the well, then according to the flow of experiment,
Chamber needed for being gated with breach ring-type instrument, presses chamber, the liquid in chamber is flowed into hybrid reaction chamber by runner
Reacted, the mixing of reaction solution can be by being spaced pressing hybrid reaction chamber and gating chamber repeatedly, can be to mixing during reaction
Reaction chamber carries out Magneto separate, and heating etc. is operated.After the elution that nucleic acid is completed in hybrid reaction chamber, augmentation detection chamber is gated
Room, the augmentation detection that multiple augmentation detection chambers carry out nucleic acid, real time inspection detection knot are pressed into by the good nucleic acid of extraction purification
Really.Greatly simplified the invention enables the equipment of detection of nucleic acids, reduce the cost of detection, make detection of nucleic acids more immediately, just
It is prompt, efficient, accurate.
Brief description of the drawings
The overall top view of Fig. 1 present invention；
The overall side view of Fig. 2 present invention；
Fig. 3 nucleic acid cleavage schematic diagrames；
Fig. 4 nucleic acid and magnetic bead cohesive process schematic diagram；
Nucleic acid amplification detects schematic diagram is carried out after Fig. 5 Nucleic Acid Elutions；
Wherein：1- substrate plates, 2- wells, 3- runners, 4- lysate chambers, 5- magnetic nanoparticle chambers, 6- is combined
Sap cavity room, 7- cleaning fluid I chambers, 8- cleaning fluid II chambers, 9- cleaning fluid III chambers, 10- hybrid reaction chambers, 11- eluents
Chamber, 12- augmentation detection chambers, 13- the barcode size or text fields, 14- breach ring-type instruments, 15- magnetic nanoparticles.
Embodiment
The utility model is done and further explained with reference to embodiment and accompanying drawing 1-5.The present embodiment, which is used, to be passed through
Introduce the detection of nucleic acids procedure declaration the utility model for being specifically based on paramagnetic particle method.This embodiment is merely to illustrate the utility model,
But it is not used to limit practical range of the present utility model.
The utility model is related to a kind of integrated closed microfluidic nucleic acid detection means, is the entirety of the present apparatus as shown in Figure 1
Top view, the utility model is mainly by a substrate plate 1, well 2 and some runners 3, and multiple chambers and bar code 13 are constituted,
All reagents before device experiment first needed for pre-packaged experiment, device entirety side view is as shown in Figure 2.
Before detection of nucleic acids experiment starts, first scan on bar code 13, software kit and corresponding parameter, such as sample class are set
Type, temperature, time etc..Then pressed using ring-type breach instrument 14 around centered on hybrid reaction chamber 10, breach side
To towards lysate chamber 4, therefore the only flow passage of lysate chamber 4 and hybrid reaction chamber 10, other runners are equal
It is blocked, sample is then injected into runner 3 subsequently into lysate chamber 4 by well 2, pressing lysate chamber 4 makes it
In liquid enter hybrid reaction chamber 10 carry out cracking reaction, as shown in Figure 3.After the completion of cracking, ring-type breach instrument is unclamped
14, its breach direction is gone to towards after magnetic nanoparticle chamber 5 again by compression, now magnetic nanoparticle chamber 5 and mixed
The flow passage of reaction chamber 10 is closed, then presses magnetic nanoparticle chamber 5, magnetic nanoparticle 15 is entered into hybrid reaction chamber
Room 10, as shown in Figure 4.Ring-type breach instrument 14 is unclamped, its breach direction is gone to towards after combination sap cavity room 6 again by compression,
Hybrid reaction chamber 10 is pressed into reference to liquid, interval pressing, which combines sap cavity room 6 and hybrid reaction chamber 10, makes the liquid in chamber
Body is fully mixed, the fully absorption nucleic acid of magnetic nanoparticle 15.After the completion of magnetic nanoparticle 15 and nucleic acid are combined, outside applies
Magnet carries out Magneto separate to hybrid reaction chamber 10, and the clear liquid waste liquid in hybrid reaction chamber 10 is then pressed into what is made the return trip empty respectively
In chamber.Its breach direction is gone to towards after cleaning fluid I chambers 7 again by compression, cleaning fluid I is pressed into hybrid reaction chamber
In 10, mixed reaction solution repeats Magneto separate operation after cleaning fully.The operation of cleaning for the first time is repeated, cleaning fluid is gated successively
II chambers and cleaning fluid III chambers are cleaned to the magnetic nanoparticle 15 in hybrid reaction chamber 10.Three cleanings are completed
Backgating eluent chamber 11, hybrid reaction chamber 10 is pressed into by eluent, to its external heat to 65 DEG C ten minutes, then
Magneto separate operation is carried out, the extraction purification of nucleic acid is completed.Augmentation detection chamber 12 is gated again, as shown in figure 5, sample of nucleic acid is pressed
Augmentation detection chamber 12 is pressed into, real-time fluorescence augmentation detection, Real Time Observation testing result is carried out.
According to above-mentioned operating process, it is convenient that the utility model can be realized, safe and efficient, inexpensive detection of nucleic acids.
Claims (7)
1. a kind of integrated closed microfluidic nucleic acid detection means, it is characterised in that including：
Substrate plate, the substrate plate is provided with multistage runner and the pre-packaged chamber for having a reagent；The chamber includes：Crack sap cavity
Room, magnetic nanoparticle chamber, with reference to sap cavity room, cleaning fluid I chambers, cleaning fluid II chambers, cleaning fluid III chambers, mixing is anti-
Answer chamber, eluent chamber, augmentation detection chamber；
Comprising the reagent needed for detection in the chamber, core chamber is hybrid reaction chamber, and other chambers are distributed in mixing
Around thing reaction chamber, provided with unique connection runner between each chamber and hybrid reaction chamber, coordinate external drive gating
Runner.
2. device according to claim 1, it is characterised in that the substrate plate is provided with a well, the well
Outside it can be sealed after injecting sample, for preventing liquid from being overflowed from well.
3. device according to claim 1, it is characterised in that the chamber is flexible, for when pressing chamber, making
Obtain the liquid outflow in chamber.
4. device according to claim 1, it is characterised in that be additionally provided with bar code on described substrate plate, for carrying out
Bar code scanning is carried out before detection of nucleic acids, the parameter needed for experiment is obtained.
5. device according to claim 4, it is characterised in that the parameter includes reaction temperature, time.
6. device according to claim 1, it is characterised in that：Augmentation detection chamber in the chamber is used provided with multiple
In the Multiple detection of nucleic acid.
7. device according to claim 1, it is characterised in that：The material of the substrate plate is dimethyl silicone polymer
(PDMS), polymethyl methacrylate (PMMA), makrolon (PC) or other polymeric materials.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621377472.XU CN206375901U (en) | 2016-12-14 | 2016-12-14 | A kind of integrated closed microfluidic nucleic acid detection means |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621377472.XU CN206375901U (en) | 2016-12-14 | 2016-12-14 | A kind of integrated closed microfluidic nucleic acid detection means |
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| CN206375901U true CN206375901U (en) | 2017-08-04 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107723210A (en) * | 2017-11-19 | 2018-02-23 | 杭州安弼晟生物科技有限公司 | Novel nucleic acids detect micro flow control chip device |
| CN107841447A (en) * | 2017-12-13 | 2018-03-27 | 上海汉谟医疗科技有限公司 | A kind of apparatus and method of extraction purification nucleic acid |
| CN107893020A (en) * | 2017-11-27 | 2018-04-10 | 深圳华炎微测医疗科技有限公司 | Molecule diagnoses micro-fluidic chip and molecule diagnosis micro-fluidic chip system and their application |
| CN108192816A (en) * | 2018-02-05 | 2018-06-22 | 宁波东夏生物科技有限公司 | A kind of sample automatically processes and nucleic acid amplifier and application method |
| CN110186742A (en) * | 2019-07-08 | 2019-08-30 | 苏州新海生物科技股份有限公司 | POCT detection device and sample evenly mixing device |
| CN111073810A (en) * | 2019-12-20 | 2020-04-28 | 深圳市华迈生物医疗科技有限公司 | Microfluidic chip, system and method integrating nucleic acid extraction, amplification and detection |
| CN112126642A (en) * | 2019-06-25 | 2020-12-25 | 京东方科技集团股份有限公司 | Nucleic acid extraction device and method thereof |
| WO2023216361A1 (en) * | 2022-05-07 | 2023-11-16 | 合肥诺迈基生物科技有限公司 | Poct micro-fluidic chip, detection system, detection method, and application |
-
2016
- 2016-12-14 CN CN201621377472.XU patent/CN206375901U/en active Active
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107723210A (en) * | 2017-11-19 | 2018-02-23 | 杭州安弼晟生物科技有限公司 | Novel nucleic acids detect micro flow control chip device |
| CN107893020A (en) * | 2017-11-27 | 2018-04-10 | 深圳华炎微测医疗科技有限公司 | Molecule diagnoses micro-fluidic chip and molecule diagnosis micro-fluidic chip system and their application |
| CN107841447A (en) * | 2017-12-13 | 2018-03-27 | 上海汉谟医疗科技有限公司 | A kind of apparatus and method of extraction purification nucleic acid |
| CN108192816A (en) * | 2018-02-05 | 2018-06-22 | 宁波东夏生物科技有限公司 | A kind of sample automatically processes and nucleic acid amplifier and application method |
| CN112126642A (en) * | 2019-06-25 | 2020-12-25 | 京东方科技集团股份有限公司 | Nucleic acid extraction device and method thereof |
| CN110186742A (en) * | 2019-07-08 | 2019-08-30 | 苏州新海生物科技股份有限公司 | POCT detection device and sample evenly mixing device |
| CN111073810A (en) * | 2019-12-20 | 2020-04-28 | 深圳市华迈生物医疗科技有限公司 | Microfluidic chip, system and method integrating nucleic acid extraction, amplification and detection |
| WO2023216361A1 (en) * | 2022-05-07 | 2023-11-16 | 合肥诺迈基生物科技有限公司 | Poct micro-fluidic chip, detection system, detection method, and application |
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Effective date of registration: 20220915 Address after: 518000 1002, building 1, Senyang Electronic Technology Park, west area, Guangming Gaoxin Park, Tianliao community, Yutang street, Guangming District, Shenzhen, Guangdong Province Patentee after: SHENZHEN LEMNISCARE MEDICAL TECHNOLOGY CO.,LTD. Address before: 518000 Room 201, building A, No. 1, Qian Wan Road, Qianhai Shenzhen Hong Kong cooperation zone, Shenzhen, Guangdong (Shenzhen Qianhai business secretary Co., Ltd.) Patentee before: SHENZHEN AIKETE BIOTECHNOLOGY CO.,LTD. |
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