CN108946927B - Sludge rapid culture method for realizing nitrite accumulation in sewage - Google Patents
Sludge rapid culture method for realizing nitrite accumulation in sewage Download PDFInfo
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Abstract
The invention discloses a sludge rapid culture method for realizing nitrite accumulation in sewage, which comprises the following steps: s1: taking a proper amount of activated sludge mixed liquor at an aerobic nitrification section of a sewage treatment plant, measuring the sludge concentration (MLSS) value of the activated sludge mixed liquor, discarding supernatant, washing the activated sludge mixed liquor twice with clear water, diluting and concentrating the activated sludge mixed liquor according to the MLSS value of the activated sludge mixed liquor to keep the sludge concentration of the activated sludge mixed liquor at 3000-4000mg/L, and adding a proper amount of sludge into a reactor; s2: adding simulated wastewater, and comparing the activity of ammonia oxidizing bacteria with the activity of nitrate bacteria; s3: selecting a proper treatment method according to the ratio, namely whether sludge domestication is carried out or not, adding sulfide with corresponding concentration, and then treating with free ammonia with certain concentration; compared with the prior art, the activated sludge treated by the method can keep higher accumulation of nitrite nitrogen for a longer period of time, has better treatment effect, is not easy to generate activity rebound of nitrate bacteria (NOB), and has more obvious inhibition effect.
Description
Technical Field
The invention belongs to the technical field of biological sewage treatment, and particularly relates to a sludge rapid culture method for realizing nitrite accumulation in sewage.
Background
The sewage treatment is one of the important problems in the environmental treatment process, and with the concern of people on the energy consumption problem in the sewage treatment process, an energy-saving sewage treatment mode is more and more popular, such as a short-cut nitrification and denitrification process, the process stays at a nitrite stage by controlling the nitrification process, on one hand, the aeration quantity is reduced, on the other hand, nitrite is directly reduced into nitrogen by denitrifying bacteria, and the supply of a carbon source is reduced, so that the process is an energy-saving sewage treatment process, in particular to the combination of the short-cut nitrification and anaerobic ammonia oxidation process, and is one of the leading-edge hotspots in the field of sewage treatment, wherein the effective proceeding of the short-cut nitrification process is the key for ensuring the anaerobic ammonia oxidation process, but the reaction process of the short-cut nitrification is difficult to control, and the accumulation of nitrite is.
Under the condition of sufficient dissolved oxygen, the activity of nitrate bacteria (NOB) is higher than that of Ammonia Oxidizing Bacteria (AOB), nitrite is converted into nitrate immediately, the concentration of nitrite in effluent is extremely low, and at present, related researches on regulating and controlling nitrite accumulation mainly focus on controlling dissolved oxygen, pH and the like of sewage in a reaction system, continuously inhibiting the nitrate bacteria and blocking a way of converting nitrite into nitrate, and in addition, part of substances such as Free Ammonia (FA), Free Nitrous Acid (FNA) and perchloric acid ClO are discovered in recent years4 -Volatile Fatty Acids (VFAs) and the like have a significantly stronger inhibitory effect on nitrate bacteria (NOB) than on Ammonia Oxidizing Bacteria (AOB), thereby enabling the control of the accumulation of nitrite nitrogen, such as: chinese patent No.: 201010508420.2, publication date: 2011.04.06 discloses a method for realizing SBR nitrosation-denitrosation at low temperature by controlling the accumulation of free ammonia, and the invention relates to a method for inhibiting the activity of Nitrobacteria (NOB) by controlling the concentration of Free Ammonia (FA) in a reaction system at low temperature (10-18 ℃) so as to achieve the accumulation of nitrite; the following steps are repeated: chinese patent No.: 201310347376.5, publication date: 2013, 11 and 20, disclose a method and a device for realizing the persistent and stable accumulation of nitrite under low temperature conditions, and the sludge is acclimated by adopting two-stage aeration and a mode of regulating and controlling the aeration intensity to achieve the effect of accumulating the nitrite, but the research generally needs more complex process control, has more strict requirements on treatment conditions, has more complex devices and has higher equipment cost. Currently, there are few reports of effective nitrite accumulation resulting from the combined treatment of activated sludge by multiple treatment methods.
Disclosure of Invention
The invention aims to provide a sludge rapid culture method for realizing nitrite accumulation in sewage, which can generate stable and long-term nitrite accumulation and provide conditions for effectively carrying out anaerobic ammonia oxidation and other processes.
The technical scheme of the invention is as follows:
a sludge rapid culture method for realizing nitrite accumulation in sewage comprises the following steps:
s1: taking a proper amount of activated sludge mixed liquor at an aerobic nitrification section of a sewage treatment plant, measuring the sludge concentration (MLSS) value of the activated sludge mixed liquor, discarding supernatant, washing the activated sludge mixed liquor twice with clear water, diluting and concentrating the activated sludge mixed liquor according to the MLSS value of the activated sludge mixed liquor to keep the sludge concentration of the activated sludge mixed liquor at 3000-4000mg/L, and adding a proper amount of sludge into a reactor;
s2: adding simulated wastewater, wherein the simulated wastewater quality is as follows: 25mg/L NH4Cl-N、25mg/L NaNO2-N and 250mg/L glucose, without adding nitrate, controlling the dissolved oxygen concentration in the reactor at 2.0mg/L, reacting for 2 hours, taking water samples every 20 minutes to detect NH in the sewage4 +-N、NO3 --N、NO2 --N content, determining the sludge biomass concentration (MLVSS) in the mixed liquor and calculating its ammonia oxidizing bacterial activity (rAOB) and nitric acid bacterial activity (rNOB) when the ammonia nitrogen and nitrous states conversion in the wastewater is complete, respectively, wherein rAOB and rNOB are expressed as follows in mg N/(g MLVSS h) and comparing their ammonia oxidizing bacterial activity (rAOB) and nitric acid bacterial activity (rNOB) activities;
s3: selecting a proper treatment method according to the ratio, namely whether sludge domestication is carried out or not, adding sulfide with corresponding concentration, and then treating with free ammonia with certain concentration.
The principle of the method is that the tolerance of Ammonia Oxidizing Bacteria (AOB) to sulfides with certain concentration is stronger than that of nitric acid bacteria (NOB), after the sulfides are removed, the ammonia oxidizing bacteria can recover quickly, so that the bacteria occupy dominant population positions, the tolerance of nitric acid bacteria (NOB) to the sulfides is poor, the activity is easily inhibited, the effect of free ammonia on two types of bacteria is similar to that of the free ammonia, after the Ammonia Oxidizing Bacteria (AOB) in aerobic sludge occupy dominant positions, ammonia nitrogen is oxidized into nitrite nitrogen and cannot be timely converted into nitrate nitrogen, so that effluent contains nitrite with higher concentration, and the two types of bacteria have better accumulated nitrite effect when being combined to treat the activated sludge.
Further, when rAOB/rNOB >0.5, the treatment was as follows:
(1) firstly, 100-150mg/L Na is adopted2S-S processing, wherein the adding mode is as follows: adding a certain amount of Na2S, adjusting the pH value to 7.2 by using 0.5mol/L hydrochloric acid or sodium hydroxide, stirring under anaerobic conditions, in the first stage, performing stirring treatment by using a magnetic stirring rod, wherein the stirring speed is 1500r/min and the stirring time is 3-5h, 45-60% of activated sludge is ensured to be in a suspended state during stirring, in the second stage, the stirring speed is 900r/min and the stirring time is 7-10h, in the third stage, the stirring speed is controlled to be 700r/min and the stirring time is 2-3h, 50-65% of activated sludge is ensured to be in a suspended state during stirring, and the temperature is controlled to be 25-45 ℃ during stirring;
(2) adding 400-450mg/L NH into an activated sludge system4Cl-N, 0.5mol/L sodium hydroxide is used for adjusting the pH value to 8.1, the concentration of free ammonia in the system is 25-32mg/L, the reactor is at the temperature of 25-30 ℃, the water inlet time is controlled to be 15min, the water outlet time is 12min, the hydraulic retention time is 25min, the aeration amount is controlled to be 0.2-0.4L/min, the stirring speed is 500-800r/min, and for NO2 -Real-time observation of the-N content when NO is present2 -When the content of-N is increased, carrying out anoxic stirring at the stirring speed of 550-700r/min, stopping stirring after 1.2-2h treatment, carrying out water-mud separation after the hydraulic retention time is 45min and the air floatation treatment is 20-35min, and discharging supernatant after 0.5h standing.
Further, when rAOB/rNOB <0.5, it is treated as follows:
(1) putting a proper amount of activated sludge into a reactor to simulate wastewater culture, controlling the low dissolved oxygen to be 1.0mg/L, controlling the temperature to be 33-35 ℃, adjusting the pH value to be 5.9-6.2, carrying out first water inflow, wherein the water inflow of the wastewater is 60-75% of the volume of the reactor, carrying out aeration treatment, and the hydraulic retention time is 7-8h, when the concentration of dissolved oxygen in the reactor rises to 2.0mg/L, stopping aeration treatment, feeding water again, wherein the entering amount of wastewater is 50-60% of the water inlet volume of the first wastewater amount, precipitating for 25-35min, discharging water, feeding water for the second time, adopting a continuous water inlet and outlet mode, feeding water gradually according to 70% of the reactor volume each time, keeping hydraulic retention time for 6-12h, continuously aerating for 4-6h, precipitating for 30-40min, and carrying out periodic culture and acclimation for 2-3 d;
(2) adopts 100-150mg/L Na2S-S processing, wherein the adding mode is as follows: adding a certain amount of Na2S, adjusting the pH value to about 7.2 by using 0.5mol/L hydrochloric acid or sodium hydroxide, stirring under anaerobic conditions, in the first stage, performing stirring treatment by using a magnetic stirring rod, wherein the stirring speed is 1500r/min and the stirring time is 3-5h, in the second stage, the stirring speed is 800-900r/min and the stirring time is 7-10h, in the third stage, the stirring speed is controlled at 700r/min and the stirring time is 2-3h, and the temperature is controlled at 25-45 ℃;
(3) adding 400-450mg/L NH4Cl-N into an activated sludge system, adjusting the pH to 8.1 by using 0.5mol/L sodium hydroxide, wherein the concentration of free ammonia in the system is 25-32mg/L, controlling the water inlet time to be 15min, the water outlet time to be 12min, the hydraulic retention time to be 25min, controlling the aeration rate to be 0.2-0.4L/min, the stirring speed to be 500-800r/min and controlling the NO to be NO2 -Real-time observation of the-N content when NO is present2 -When the content of-N is increased, carrying out anoxic stirring at the stirring speed of 550-700r/min, stopping stirring after 1.2-2h, staying for 45min by hydraulic power, carrying out air floatation treatment for 20-35min for mud-water separation, and discharging supernatant after 0.5h of standing. Compared with the prior art, the invention has the beneficial effects that:
(1) according to the invention, the enrichment treatment of ammonia oxidizing bacteria is carried out on the sludge with low ammonia oxidizing bacteria activity, and the combined action of sulfide and free ammonia on inhibiting aerobic microorganisms is adopted, so that the activities and population changes of ammonia oxidizing bacteria and nitric acid bacteria in the activated sludge are regulated and controlled, higher nitrite accumulation is generated, complicated operation parameter control is not needed, the treatment conditions are easy to achieve, and the method can be better applied to the current engineering application;
(2) compared with the prior art, the activated sludge treated by the method can keep higher accumulation of nitrite nitrogen for a longer period of time, has better treatment effect, is not easy to generate activity rebound of nitrate bacteria (NOB), and has more obvious inhibition effect.
Drawings
FIG. 1 is a block flow diagram of the present invention.
Detailed Description
For a further understanding of the present invention, reference is made to FIG. 1 and the following examples.
Example 1
This example relates to a method for treating activated sludge for accumulation of nitrite when the activated sludge has a high activity of ammonia oxidizing bacteria after concentration or dilution after washing, i.e., rAOB/rNOB > 0.5.
The activated sludge is activated sludge of an aerobic section of a certain sewage treatment plant in Nanjing, the concentration of the activated sludge is measured to be 3500mg/L, dilution or concentration is not needed, the original sludge concentration is maintained after washing twice by clear water, the rAOB/rNOB value is measured to be 0.58 to 0.5, enrichment of ammonia oxidizing bacteria is not needed, and meanwhile, an activated sludge system only carrying out treatment on free ammonia with the same concentration is used for comparison.
Adding Na to the reactor2S to make the concentration of the Na to be 100mg/L2S-S, adding a certain amount of Na2S, adjusting the pH value to about 7.2 by using 0.5mol/L hydrochloric acid, stirring under anaerobic conditions, in the first stage, stirring by using a magnetic stirring rod, wherein the stirring speed is 1000r/min, the stirring time is 3 hours, 45% of activated sludge is ensured to be in a suspension state during stirring, in the second stage, the stirring speed is 800r/min, the stirring time is 7 hours, in the third stage, the stirring speed is controlled to be 500r/min, the stirring time is 2 hours, 50% of activated sludge is ensured to be in a suspension state during stirring, the temperature is controlled to be 25 ℃, and then 400mg/L NH is added into an activated sludge system4Cl-N, and adjusting the pH to 8.1 with 0.5mol/L sodium hydroxide, which corresponds to a free ammonia concentration of 2 in the system5mg/L, the reactor is carried out at the temperature of 25 ℃, the water inlet time is controlled to be 15min, the water outlet time is controlled to be 12min, the hydraulic retention time is 25min, the aeration rate is controlled to be 0.2L/min, the stirring speed is 500r/min, and NO is treated2 -Real-time observation of the-N content when NO is present2 -When the content of-N is increased, carrying out anoxic stirring at the stirring speed of 550r/min, stopping stirring after 1.2h of treatment, carrying out hydraulic retention time of 45min, carrying out air flotation treatment for 20min, carrying out mud-water separation, standing for 0.5h, and discharging supernatant.
In 1-6 days after the reactor normally operates, the dissolved oxygen of the activated sludge system treated by sulfide and free ammonia is controlled to be 1mg/L, the accumulation rate of nitrite in effluent (effluent nitrite nitrogen/effluent nitrite nitrogen + nitrate nitrogen) gradually rises to 85 percent and reaches stability, the dissolved oxygen of the activated sludge system only treated by the free ammonia keeps approximately the same as that of an experimental group after inoculation, the accumulation rate of nitrite nitrogen in effluent rises to be maintained at 62 percent in the first two days and then gradually falls to about 35 percent, and the activated sludge system only treated by the free ammonia reaches stability in 11 days, and the result shows that the mixed treatment of sulfide and free ammonia can effectively inhibit the activity of nitrate bacteria (NOB), so that Ammonia Oxidizing Bacteria (AOB) become dominant species and reach a more stable nitrite accumulation effect, and the time for achieving stable short-cut nitrification is shortened by 5 days, and the short-range nitration effect is better.
Example 2
This example is a treatment method in which activated sludge is treated to form an accumulation of nitrite nitrogen under conditions where the activated sludge has low activity of Ammonia Oxidizing Bacteria (AOB), i.e., rabo/rNOB < 0.5.
The activated sludge is activated sludge of an aerobic nitrification reaction section of a certain sewage treatment plant in Nanjing, the concentration of the sludge is determined to be 5200mg/L, the activated sludge is diluted to 3000mg/L according to the proportion after being washed by clear water, and the rAOB/rNOB value is determined to be 0.41<0.5, putting a proper amount of activated sludge into the reactor to simulate wastewater culture, controlling the low dissolved oxygen to be 1.0mg/L, controlling the temperature to be 35 ℃, adjusting the pH value to be 6.2, feeding water for the first time, wherein the water inflow of the wastewater is 60 percent of the volume of the reactor, carrying out aeration treatment, the hydraulic retention time is 8 hours, and when the reactor is filled with water, the reactor is filled with activated sludgeThe concentration of dissolved oxygen is increased to 2.0mg/L, then aeration treatment is stopped, water is fed again, the inlet amount of wastewater is 55 percent of the volume of the first wastewater, precipitation is carried out for 35min, water is discharged, water is fed for the second time, a continuous water inlet and outlet mode is adopted, water is fed gradually according to 70 percent of the volume of a reactor each time, the hydraulic retention time is 12h, aeration is continued for 6h, precipitation is carried out for 40min, periodic culture and acclimatization are carried out for 3d, Na is added into the reactor2S to make the concentration reach 150mg/L Na2S-S, adjusting the pH value to 7.2 by adopting 0.5mol/L hydrochloric acid, stirring under anaerobic conditions, stirring by using a magnetic stirring rod at the first stage of 1500r/min for 5h, stirring at 900r/min for 10h, stirring at 700r/min for 3h at 45 ℃, and adding 450mg/L NH into an activated sludge system4Cl-N, and 0.5mol/L sodium hydroxide is used for adjusting the pH value to 8.1, which is equivalent to that the concentration of free ammonia in the system is 32mg/L (which is higher concentration), the reactor is at the temperature of 30 ℃, the water inlet time is controlled to be 15min, the water outlet time is 12min, the hydraulic retention time is 25min, the aeration amount is controlled to be 0.4L/min, the stirring speed is 800r/min, and for NO2 -Real-time observation of the-N content when NO is present2 -And when the content of the N is increased, carrying out anoxic stirring at the stirring speed of 700r/min, stopping stirring after treating for 2 hours, staying for 45 minutes under hydraulic power, carrying out air floatation treatment for 35 minutes, carrying out sludge-water separation after standing for 0.5 hour, discharging supernatant, adding wastewater with the ammonia nitrogen concentration of 80mg/L and the COD of 420mg/L as inlet water, and carrying out normal short-cut nitrification treatment. Meanwhile, activated sludge treated with only free ammonia was used as a control.
Respectively inoculating the activated sludge of the experimental group and the activated sludge of the control group to the corresponding SBR, setting aeration intensity, controlling the dissolved oxygen of the activated sludge to be between 1 and 2mg/L, controlling the temperature to be 20 ℃, controlling the Hydraulic Retention Time (HRT) to be 6 hours, controlling the pH value in the reaction system to be between 6.5 and 7.5, and operating for a period of time.
After inoculation in a reactor, the active sludge system is treated by mixing sulfide and free ammonia within 1 to 7 days, the cumulative rate of the effluent nitrite nitrogen (effluent nitrite nitrogen/effluent nitrite nitrogen + nitrate nitrogen) gradually rises to 81 percent and reaches a stable state, while in the control group, namely, in the activated sludge system only treated by free ammonia, the accumulation rate of nitrite nitrogen in effluent is gradually increased to 64 percent within 1-12d and then gradually decreased to 40 percent, the experimental result shows that the mixed treatment of sulfide and free ammonia can effectively inhibit the activity of nitrate bacteria (NOB), can reach relatively high accumulation rate of nitrite within a short time, compared with the activated sludge only treated by free ammonia, the time for achieving short-cut nitrification is shortened by 5 days, and the reactor can stably run for one week after the combined treatment of sulfide and free ammonia.
Example 3
This example is a treatment process for activated sludge under conditions where the activated sludge has a high activity of Ammonia Oxidizing Bacteria (AOB), i.e., rAOB/rNOB > 0.5.
The activated sludge is activated sludge of an aerobic nitrification reaction section of a certain sewage treatment plant in Nanjing, the concentration of the sludge is measured to be 2600mg/L, the activated sludge is concentrated to 4000mg/L according to the proportion after being washed by clear water, and the rAOB/rNOB value is measured to be 0.62>0.5 addition of Na to the reactor without enrichment of Ammonia Oxidizing Bacteria (AOB)2S to make the concentration of the sodium sulfate reach 125mg/L Na2S-S, adjusting the pH value to 7.2 by adopting 0.5mol/L hydrochloric acid, stirring under anaerobic conditions, in the first stage, stirring by using a magnetic stirring rod, wherein the stirring speed is 1300r/min, the stirring time is 4h, 50% of activated sludge is ensured to be in a suspension state during stirring, in the second stage, the stirring speed is 850r/min, the stirring time is 8h, in the third stage, the stirring speed is controlled to be 600r/min, the stirring time is 2h, 55% of activated sludge is ensured to be in a suspension state during stirring, the temperature is controlled to be 30 ℃, and then 425mg/L NH is added into an activated sludge system4Cl-N, and 0.5mol/L sodium hydroxide is used for adjusting the pH value to 8.1, namely the concentration of free ammonia in the system is changed to 30mg/L, the reactor is controlled at the temperature of 28 ℃, the water inlet time is 15min, the water outlet time is 12min, the hydraulic retention time is 25min, the aeration rate is controlled to be 0.3L/min, the stirring speed is 700r/min, and for NO2 -Real-time observation of the-N content when NO is present2 -Increased content of-NAnd (3) performing anoxic stirring at the stirring speed of 650r/min for many times, stopping stirring after 1.8h treatment, performing hydraulic retention for 45min, performing sludge-water separation after 25min of air flotation treatment, standing for 0.5h, discharging supernatant, adding wastewater with the ammonia nitrogen concentration of 40mg/L and the COD of 220mg/L as inlet water, performing normal short-range nitrification treatment, and taking activated sludge only subjected to free ammonia treatment as a control.
After the activated sludge system normally runs in a reactor, the accumulation rate of nitrite nitrogen in effluent (effluent nitrite nitrogen/effluent nitrite nitrogen + nitrate nitrogen) of the activated sludge system treated by mixing sulfide and free ammonia gradually rises to 89% within 1-7 days, and the effluent is stable, while the accumulation rate of nitrite nitrogen in effluent gradually rises to 72% within 1-11 days and then gradually falls to 52% in a control group, namely the activated sludge system treated by only free ammonia, and experimental results show that the mixed treatment of sulfide and free ammonia can effectively inhibit the activity of nitric acid bacteria (NOB), and the accumulated rate of nitrite can reach a relatively high accumulation rate within a short time after the treatment of free ammonia, and the time for achieving stable short-cut nitrification is shortened by 4 days.
It should be noted that, for those skilled in the art, in light of the present disclosure and the specific embodiments thereof, modifications can be made and still other methods can be used to implement the functions and effects described in the present invention without departing from the scope of the present invention.
Claims (2)
1. A sludge rapid culture method for realizing sewage nitrite accumulation is characterized by comprising the following steps:
s1: taking a proper amount of activated sludge mixed liquor at an aerobic nitrification section of a sewage treatment plant, measuring the MLSS value of the activated sludge mixed liquor, removing supernatant, washing twice with clear water, diluting and concentrating the activated sludge mixed liquor according to the MLSS value to keep the sludge concentration of the activated sludge mixed liquor at 3000-4000mg/L, and adding a proper amount of sludge into a reactor;
s2: adding simulated wastewater, wherein the simulated wastewater quality is as follows: 25mg/L NH4Cl-N、25mg/L NaNO2-N and 250mg/L glucose, without adding nitrate, controlling the dissolved oxygen concentration in the reactor at 2.0mg/L, reacting for 2 hours, taking water samples every 20 minutes to detect NH in the sewage4 +-N、NO3 --N、NO2 --N content, determining the sludge biomass concentration MLVSS in the mixed liquor, and calculating its ammonia oxidizing bacteria activity rAOB and nitric acid bacteria activity rNOB when the ammonia nitrogen and nitrous state conversion in the sewage is complete, respectively, wherein rAOB and rNOB are expressed as follows, in mg N/(g MLVSS h), and comparing their ammonia oxidizing bacteria activity rAOB with their nitric acid bacteria activity rNOB;
s3: selecting a proper treatment method according to the ratio, namely whether sludge domestication is carried out or not, adding sulfide with corresponding concentration, and then treating with free ammonia with certain concentration;
in the processing method of step S3, raab/rNOB >0.5 is processed as follows:
(1) firstly, 100-150mg/L Na is adopted2S-S processing, wherein the adding mode is as follows: adding a certain amount of Na2S, adjusting the pH value to 7.2 by using 0.5mol/L hydrochloric acid or sodium hydroxide, stirring under anaerobic conditions, in the first stage, stirring by using a magnetic stirring rod at the stirring speed of 1000-1500r/min for 3-5h while ensuring that 45-60% of the activated sludge is in a suspended state, in the second stage, stirring at the stirring speed of 800-900r/min for 7-10h, in the third stage, controlling the stirring speed at 700r/min for 500-3 h while ensuring that 50-65% of the activated sludge is in a suspended state while stirring, and controlling the temperature to 25-45℃；
(2) Adding 400-450mg/L NH into an activated sludge system4Cl-N, 0.5mol/L sodium hydroxide is used for adjusting the pH value to 8.1, the concentration of free ammonia in the system is 25-32mg/L, the reactor is at the temperature of 25-30 ℃, the water inlet time is controlled to be 15min, the water outlet time is 12min, the hydraulic retention time is 25min, the aeration amount is controlled to be 0.2-0.4L/min, the stirring speed is 500-800r/min, and for NO2 -Real-time observation of-N content, NO2 -Increasing the content of N, carrying out anoxic stirring at the stirring speed of 550-700r/min, stopping stirring after treating for 1.2-2h, keeping the hydraulic retention time for 45min, carrying out mud-water separation after air flotation treatment for 20-35min, and discharging supernatant after standing for 0.5 h;
in the processing method of step S3, raab/rNOB <0.5, the processing is as follows:
(1) putting a proper amount of activated sludge into a reactor to simulate wastewater culture, controlling the low dissolved oxygen to be 1.0mg/L, controlling the temperature to be 33-35 ℃, adjusting the pH value to be 5.9-6.2, feeding water for the first time, wherein the water inflow of the wastewater is 60-75% of the volume of the reactor, carrying out aeration treatment, wherein the hydraulic retention time is 7-8h, the concentration of the dissolved oxygen in the reactor is increased to 2.0mg/L, stopping the aeration treatment, feeding water again, the feeding amount of the wastewater is 50-60% of the volume of the wastewater for the first time, carrying out precipitation for 25-35min, draining, feeding water for the second time, adopting a continuous water feeding and discharging mode, feeding water gradually according to 70% of the volume of the reactor each time, carrying out hydraulic retention time to be 6-12h, continuously aerating for 4-6h, precipitating for 30-40min, and carrying out periodic culture and acclimation for 2-3 d;
(2) adopts 100-150mg/L Na2S-S processing, wherein the adding mode is as follows: adding a certain amount of Na2S, adjusting the pH value to 7.2 by using 0.5mol/L hydrochloric acid or sodium hydroxide, stirring under anaerobic conditions, in the first stage, performing stirring treatment by using a magnetic stirring rod, wherein the stirring speed is 1500r/min and the stirring time is 3-5h, in the second stage, the stirring speed is 800-;
(3) adding 400-450mg/L NH into an activated sludge system4Cl-N, 0.5mol/L sodium hydroxide is used for adjusting the pH value to 8.1, the concentration of free ammonia in the system is 25-32mg/L, the reactor is at the temperature of 25-30 ℃, the water inlet time is controlled to be 15min, the water outlet time is 12min, the hydraulic retention time is 25min, the aeration amount is controlled to be 0.2-0.4L/min, the stirring speed is 500-800r/min, and for NO2 -Real-time observation of-N content, NO2 -Increasing the content of-N, carrying out anoxic stirring at the stirring speed of 550-700r/min, stopping stirring after 1.2-2h, staying for 45min by using hydraulic power, carrying out sludge-water separation after 20-35min by air floatation treatment, and discharging supernatant after 0.5h of standing.
2. The method for rapidly culturing the sludge for realizing the nitrite accumulation in the sewage according to claim 1, wherein the pH value in the step (1) is adjusted to 7.2 by using 0.5mol/L of sodium hydroxide.
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| CN110921820B (en) * | 2019-12-25 | 2021-12-03 | 北京工业大学 | Method for quickly starting normal-temperature short-cut nitrification of municipal sewage by using benzethonium chloride |
| CN111470614B (en) * | 2020-05-06 | 2022-03-29 | 北京工业大学 | Preparation and application of high-accumulation-rate short-cut nitrifying composite bacteria embedded bioactive filler |
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