KR100644854B1 - Method and apparatus for enriching anaerobic ammonium oxidizing bacteria continuously - Google Patents

Abstract

A method and apparatus for enriching anaerobic ammonium-oxidizing bacteria continuously are provided to significantly reduce the growth time of anaerobic ammonium-oxidizing bacteria, so that the initial operation time of wastewater treatment process is reduced. The method for enriching anaerobic ammonium-oxidizing bacteria continuously comprises the steps of: introducing the culture medium into an upflow reactor having anaerobic ammonium-oxidizing bacteria and anaerobic granular sludge and culturing the anaerobic ammonium-oxidizing bacteria(S1); introducing the total or a part of the releasing water from the upflow reactor into loss-preventing culture vessel of the anaerobic ammonium-oxidizing bacteria which is packed with nonwoven fabrics having the positive charge, and culturing the anaerobic ammonium-oxidizing bacteria(S2); and introducing the releasing water from the culture vessel into the reactor of the step(S1), wherein the retention time of solids in the upflow reactor is 50 days or more. The apparatus for enriching anaerobic ammonium-oxidizing bacteria continuously comprises the upflow reactor(10) and the loss-preventing culture vessel of anaerobic ammonium-oxidizing bacteria(20).

Description

METHOD AND APPARATUS FOR ENRICHING ANAEROBIC AMMONIUM OXIDIZING BACTERIA CONTINUOUSLY}

1 is a schematic view showing the configuration of a continuous culture apparatus of anaerobic ammonium oxide bacteria according to the present invention.

* Explanation of symbols on the main parts of the drawings *

1: Upflow Reactor 2: Anaerobic Ammonium Oxide Bacteria Loss Prevention Culture Tank

3: inflow pump 4: recirculation pump

5: sedimentation tank 6: generated nitrogen gas

7: redox potential meter 8: air ingress protector

The present invention relates to a method and apparatus for continuously culturing anaerobic ammonium oxidizing bacteria. The present invention relates to wastewater containing high concentrations of nitrogen such as reflux water, landfill leachate, and livestock wastewater generated after anaerobic treatment of treatment-producing sludge such as sewage and wastewater. The present invention relates to a method and apparatus for continuously culturing anaerobic ammonium oxidizing bacteria required for treatment.

Conventional combinations of conventional nitrification / denitrification reactions have been most commonly used for nitrogen removal of sewage, wastewater, and the like. In particular, in the case of sewage treatment, many nitrification / denitrification reaction processes have been used.

The biological nitrification is a reaction in which heterotrophic nitrification microorganisms use oxygen as an electron acceptor to oxidize ammonia nitrogen to nitrate nitrogen, which is reduced to nitrogen gas by denitrifying microorganisms under anoxic conditions. [Scheme 1] is a nitrification reaction for oxidizing ammonia nitrogen to nitrate nitrogen, and [Scheme 2] is a denitrification reaction for reducing nitrate nitrogen to nitrogen gas.

^{_{NH 4 + + 2O 2 → NO}} 3 - + 2H + + H 2 O

^{_{6NO 3 - + 5CH 3 OH +}} CO 2 → 3N 2 + 6HCO 3 - + 7H 2 O

As can be seen from [Scheme 1], in the case of nitrification, 2 moles of oxygen are consumed to oxidize 1 mole of ammonia nitrogen to nitrate nitrogen. As can be seen from [Scheme 2], in the case of denitrification 5 moles of methanol are needed to reduce 6 moles of nitrate nitrogen. On the other hand, in general, the COD / N ratio of the influent wastewater for efficiently removing nitrogen in the biological nitrogen removal process using the principles of the schemes is required 3 to 6.

In such nitrification / denitrification reaction, efficient oxygen supply and alkalinity supply are essential. Especially, since additional denitrification microorganisms require a large amount of external carbon source as electron donor, it requires not only energy but also large amount of organic material. There is a disadvantage in that a large operating cost is required for processing. Furthermore, unlike sewage having a concentration of ammonia nitrogen in the inflow water of 100 ppm or less, there is a problem in that the treatment of wastewater containing a high concentration of nitrogen requires a huge cost.

As a process for reducing the energy consumption and the amount of the organic source according to the denitrification after nitrification, a process of oxidizing ammonia nitrogen in wastewater to nitrite nitrogen and then denitrifying nitrite nitrogen is known.

This process is called the so-called Single reactor system for High activity Ammonia Removal over Nitrite (SHARON) process. The following schemes represent the reaction schemes of the SHARON process reaction.

^{_{NH 4 + + 1.5O 2 ->}} NO 2 - + H 2 O + 2H +

^{_{6NO 2 - + 3CH 3 OH +}} 3CO 2 -> 3N 2 + 6HCO 3 - + 3H 2 O

The SHARON reaction is to partially oxidize ammonia nitrogen to nitrite nitrogen by inhibiting growth in the reactor of nitrobacter, which is a nitrite nitrogen oxidizing bacterium, by adjusting the hydraulic residence time, pH and temperature of the biological reactor. As can be seen from the reaction schemes 3 and 4, the oxygen requirement of 25% compared with the conventional complete nitrification process is reduced, and the organic carbon source of 40% compared with the conventional denitrification process is advantageous.

However, the SHRON method is currently not actively commercialized because it is very difficult to stably partially oxidize ammonia nitrogen to nitrite nitrogen in actual wastewater treatment.

On the other hand, since it was recently observed that ammonia nitrogen was removed from a denitrification fluidized bed reactor treating the effluent of a methane production reactor (see US Patent No. 5,078,884), anaerobic conversion of ammonia nitrogen and nitrite nitrogen to nitrogen gas was performed. The presence of ammonium oxide was confirmed. [Scheme 5] is a reaction equation showing a nitrogen gas conversion reaction between ammonia nitrogen and nitrite nitrogen by anaerobic ammonium oxide, and [Scheme 6] is a general reaction equation.

_{^{1.0NH 4 + + 1.32NO 2 - +}} 0.066HCO 3 - + 0.13H + -> 1.02N 2 + 0.26NO 3 - + 0.066CH 2 O 0.5 N 0.15 + 2.03H 2 O

NH ₄ ⁺ + 0.75 O _2- > 0.5 N ₂

As can be seen from the above schemes, when nitrogen is removed by anaerobic ammonium oxidation by anaerobic ammonium oxidizing bacteria, only 37.5% of oxygen is required as compared to conventional nitrogen removal process, and external nitrogen required for denitrification The supply of carbon sources is not necessary at all. Therefore, the operating cost is drastically reduced and very economical.

However, the anaerobic ammonium oxidizing bacterium has a disadvantage in that it is difficult to culture in a general natural environment because the growth rate of the microorganisms is 11 to 12 days as well as the growth rate is very difficult.

In other words, it is known that incubation period of 6 months to 1 year to operate the anaerobic ammonium oxide bacteria having a microbial doubling time of 11 to 12 days at the activity and nitrogen load 0.4 kg N / m ³ -day or more applicable to the actual process This means that even if the design and manufacture of the biological nitrogen removal process is successful, the commissioning period for normal operation will be 6 months or more, so the commercial application is impossible or very difficult.

Therefore, in the conventional nitrogen removal process using anaerobic ammonium oxide bacteria, it is necessary to secure a technology capable of stably culturing the anaerobic ammonium oxide bacteria in a short time.

Accordingly, the present invention is to solve the problems of the prior art as described above, an object of the present invention is to provide an anaerobic ammonium oxide bacterium capable of converting ammonia nitrogen and nitrite nitrogen into nitrogen gas under anaerobic or anoxic conditions. In culturing, it provides a method and apparatus for continuously culturing anaerobic ammonium oxidizing bacteria which can be used for nitrogen removal by culturing the anaerobic ammonium oxidizing bacteria which have a long time for microbial culturing and difficult growing conditions.

In order to achieve the above object, the present invention provides a continuous culture method of anaerobic ammonium oxidizing bacteria, in which a culture medium is introduced into an upflow reactor in which microorganisms having anaerobic ammonium oxidizing bacteria and anaerobic granular sludge are planted, and anaerobic ammonium oxidation Culturing the bacteria (S1); Culturing anaerobic ammonium oxidizing bacteria by injecting a part or all of the effluent from the reactor of step S1 into the anaerobic ammonium oxidizing bacteria loss prevention culture tank filled with a positively charged nonwoven fabric (S2); And it is characterized in that it comprises the step (S3) of introducing the effluent from the culture tank of the step S2 back to the reactor of the step S1.

The method according to the invention is characterized in that for using the reactor in which the solid residence time is maintained for at least 50 days as the reactor in the step S1.

In the method according to the present invention, in the step S1, 1 to 3 mm of anaerobic granular sludge is planted in the reactor at least 50% of the total reactor volume and microorganisms having an activity of anaerobic ammonium oxidizing bacteria are planted to 10% of the total reactor volume. Characterized in that.

The method according to the invention, characterized in that for maintaining the flow rate of the effluent of the step S1 to 50m / hr or more.

The method according to the invention is characterized in that the redox potential in the reactor is maintained at -100 to -150 mV within 30 days of culturing the anaerobic ammonium oxidizing bacteria in the step S1.

The method according to the present invention is characterized in that in the step S1 by adding hydrogen sulfide and hydrazine to the reactor to control the redox potential.

The method according to the present invention is characterized in that the redox potential in the reactor is maintained at -30 to -100 mV after 30 days of culturing the anaerobic ammonium oxidizing bacteria in the step S1.

The method according to the invention is characterized in that the redox potential is adjusted by adjusting the molar ratio of ammonia nitrogen / nitrite nitrogen introduced into the reactor in the step S1 to 0.9 to 0.95.

The present invention for achieving the above object, as a continuous culture device of anaerobic ammonium oxidizing bacteria, microorganisms and anaerobic granular sludge with the activity of anaerobic ammonium oxidizing bacteria is planted, the culture solution is introduced into the anaerobic ammonium oxidizing bacteria is cultured Upflow reactor; And an anaerobic ammonium oxide bacteria loss prevention culture tank in which part or all of the effluent from the reactor is introduced, and a positively charged nonwoven fabric is filled, and the discharged effluent is introduced into the reactor again.

The device according to the invention is characterized in that the solid residence time of the reactor is maintained for at least 50 days.

The apparatus according to the present invention is characterized in that 1 to 3 mm of anaerobic granular sludge is planted in the reactor at least 50% of the total reactor volume, and microorganisms with the activity of anaerobic ammonium oxidizing bacteria are planted to 10% of the total reactor volume. It is done.

The apparatus according to the present invention is characterized in that the flow rate of the effluent of the reaction tank is 50m / hr or more.

The apparatus according to the present invention is characterized in that the redox potential in the reactor is maintained at -100 to -150 mV within 30 days of culturing the anaerobic ammonium oxide.

The device according to the invention is characterized in that hydrogen sulfide and hydrazine are added to the reactor.

The apparatus according to the present invention is characterized in that the redox potential in the reactor is maintained at -30 to -100 mV after 30 days of culture of the anaerobic ammonium oxide.

The apparatus according to the invention is characterized in that the molar ratio of ammonia nitrogen / nitrite nitrogen introduced into the reactor is 0.9 to 0.95.

Hereinafter, the method and apparatus for continuous culture of anaerobic ammonium oxide bacteria according to the present invention will be described in detail.

1 is a schematic view showing the configuration of a continuous culture apparatus of anaerobic ammonium oxide bacteria according to the present invention.

As shown in Figure 1, the continuous culture apparatus of anaerobic ammonium oxidizing bacteria according to the present invention is a part or all of the upflow reactor 10 and the effluent water from the upflow reactor 10 is introduced into the culture medium by the inflow pump 30 is recycle pump The anaerobic ammonium oxide bacteria loss prevention culture tank 20 which flows in by 40 is provided. The upflow reactor 10 is provided with a settling tank 50 at the top, the redox potentiometer 70 is mounted on the settling tank 50, the nitrogen gas 60 generated from the settling tank 50 is discharged. Outflow water passing through the settling tank 50 is passed through the air inlet protector 80 to the outside.

In the upflow reactor 10, microorganisms having the activity of anaerobic ammonium oxidizing bacteria are planted, and anaerobic granular sludge is planted. The culture solution is introduced into the upflow reactor 10, and anaerobic ammonium oxide bacteria are cultured. As the upflow reactor 10, an upflow continuous flow reactor having a solids residence time of 50 days or more is preferable. When the microorganism retention time is 50 days or more, growth conditions capable of stably growing anaerobic ammonium oxide bacteria having extremely slow growth rates may be provided.

The anaerobic ammonium oxide bacteria loss prevention culture tank 20 is filled with a non-woven fabric having a positive charge therein. By providing a positively charged nonwoven fabric in this manner, it is possible to facilitate the attachment of the negatively charged anaerobic ammonium oxide bacteria. Accordingly, when the effluent from the reaction tank 10 is introduced into the culture tank 20, it is possible to minimize the loss of anaerobic ammonium oxide bacteria in the effluent. At this time, for example, polypropylene is used as a material of the nonwoven fabric.

As such, the present invention employs an upflow reactor, and provides growth conditions for the growth of anaerobic ammonium oxidizing bacteria by maintaining the microorganism residence time in the upflow reactor for a predetermined time or more. In addition, the front end of the reactor is equipped with an anaerobic ammonium oxide bacterium prevention culture tank that can prevent the loss of anaerobic ammonium oxide bacteria, so that part or all of the effluent passing through the reactor is recycled without loss. Allow culture. As a result, anaerobic ammonium oxidizing bacteria having a long doubling time and difficult growing conditions can be continuously cultured in a short time.

Moreover, in the reactor, at the beginning of the culture, anaerobic granular sludge of 1 to 3 mm is planted at least 50% of the total reactor volume, and microorganisms having anaerobic oxidizing bacteria are planted to 10% of the total reactor volume within 3 months after the culture. It can be made to exhibit a nitrogen removal activity of 1kgN / m ³ -d.

In the reactor, the effluent from the reactor is maintained at 50 m / hr or more to increase the substrate contact rate of the anaerobic ammonium oxide bacteria with ammonium nitrogen and to facilitate the discharge of by-product nitrogen gas. As a result, the concentrations of the introduced ammonia nitrogen and the nitrite nitrogen can be diluted, thereby reducing the inhibitory nutrition and increasing the activity early in the initial culture.

In addition, in order to maintain optimal growth conditions of anaerobic ammonium oxidizing bacteria, the redox potential in the reactor is maintained at -100 to -150 mV or less within 30 days of culture. As such, when the redox potential is adjusted, anaerobic ammonium oxide shows excellent activity in comparison with the case where it is not. At this time, in order to control the redox potential in the above range, hydrogen sulfide and hydrazine are added to the reactor as a reducing agent in the early stage of culturing anaerobic ammonium oxide.

On the other hand, in the initial operating conditions where the activity of the anaerobic ammonium oxidants is relatively low, the optimum environment for the growth of the anaerobic ammonium oxidants is important. Sufficient alkalinity, supply of trace metals such as iron and manganese is also required.

Furthermore, after one month of continuous culture in the reactor, the activity of anaerobic ammonium oxidizing bacteria can be increased more quickly by setting the redox potential to -30 to -100 mV. In this case, in order to control the redox potential, the molar ratio of ammonia nitrogen to nitrite nitrogen is in the range of 0.9 to 0.95 instead of the injection of a reducing agent, and thus redox due to the lack of nitrite nitrogen in the reactor. The potential can be operated in the range of -30 to -100 mV.

Hereinafter, the present invention will be described in more detail by explaining preferred embodiments of the present invention. However, the present invention is not limited to the following examples, and various forms of embodiments can be implemented within the scope of the appended claims, and the following examples are only common in the art while making the disclosure of the present invention complete. It is intended to facilitate the implementation of the invention to those with knowledge.

Synthetic medium preparation

In order to evaluate the anaerobic ammonium oxidizing bacteria culture apparatus according to the present example and the comparative example, a synthetic medium was prepared and used as shown in Table 1 below.

Water (g / L) Target concentration (mg / L) (NH ₄ ) ₂ SO ₄ 0.330 70 NaNO ₂ 0.345 70 NaHCO ₃ 0.504 72 KH ₂ PO ₄ 0.027 6 MgSO _{4 7} H ₂ O 0.123 12 CaCl ₂ · 2H ₂ O 0.176 48 Hydrazine _{(N 2 H 4 · H 2} O) 0.025 14.0 Hydroxylamine (NH ₂ OH) 0.035 0.5 Trace Component Solution I * 1ml / L Trace Component Solution II ** 1ml / L Trace Component Solution I * EDTA 5 FeSO ₄ 7H ₂ O 5 Trace Component Solution II ** ZnSO ₄ · 7H ₂ O 0.430 CoCl ₂ · 6H ₂ O 0.240 MnCl ₂ 4H ₂ O 0.990 CuSO ₄ · 5H ₂ O 0.250 Na ₂ MoO ₄ 2H ₂ O 0.220 NiCl ₂ · 6H ₂ O 0.190 Na ₂ SeO ₄ · 10H ₂ O 0.210 H ₃ BO ₃ 0.014

-Production of culture device-

The culture apparatus of the Example and the comparative example was produced as follows, respectively.

An apparatus capable of continuously culturing anaerobic ammonium oxide was produced. An upflow continuous flow reactor having an effective volume of 3.35 L was made of acrylic, and a continuous culture apparatus was constructed by connecting a culture vessel filled with a nonwoven fabric made of polypropylene to the front end of the upflow continuous flow reactor (Example). A synthetic medium prepared as a culture medium was introduced into the reactor through a pump, and a recycle was performed between the culture tank and the reactor through a pump. A precipitation tank was installed at the top of the reactor, and a redox potential meter was installed at the precipitation tank. Nitrogen gas generated in the sedimentation tank was discharged and the generated gas was separated by gas solid separator (GSS) at the top of the reactor, and even a small amount of generated gas could be accurately measured by a wet gas meter (TG05 type, Ritter). It was made. Effluent from the settling tank was allowed to flow through the air inlet protector.

On the other hand, in order to compare the performance with the continuous culture device according to the embodiment, the device was configured in the same manner as in the above embodiment, but the culture device was produced without connecting the culture tank (comparative example).

In order to control the temperature of each reactor of the above Examples and Comparative Examples, a constant temperature device equipped with a PID control heater in a box of 127 cm (width) × 70 cm (length) × 151 cm (height) was manufactured and The reactor was placed.

In addition, an automatic device (Model IMC61, Isteck Inc.) and a computer for constant monitoring of pH, temperature, and ORP, which affect the growth of microorganisms having anaerobic ammonium oxide bacteria, were installed around each reactor.

Anaerobic granular sludge collected from the UASB (Upflow Anaerobic Sludge Blanket) process for drinking wastewater was used as a seedling microorganism. The anaerobic granular sludge was charged to 50% of the total volume of each reactor of the examples and comparative examples.

Microorganisms with the Activity of Anaerobic Ammonium Oxides Brocardia Anammoxidans ) was charged to 10% of the total volume of each reactor of the examples and comparative examples.

The lower portion of each reactor was filled with a volume of about 90 mL of ceramic balls having a diameter of 7 to 10 mm to prevent loss of seed microorganisms, and prepared in each reactor by using a micro pump (Model QSY with RH, Fluidmetering Inc.). One synthetic medium was introduced.

Driving and measuring

The culture apparatus of Examples and Comparative Examples was operated and measured as follows.

It was operated for 1 day at the beginning of operation as a hydraulic retention time. The internal circulation was circulated above 10Q relative to the inflow flow rate and the upward flow rate in each reactor was maintained at 50 m / hr or more.

The initial inlet nitrogen load was about 0.14 kg N / m ³ -day (synthetic medium was prepared to be so cultured) and the pH was not artificially controlled. The thermostat was operated so that the temperature in the reactor was 35 ± 1 ° C. at moderate temperature.

Effluents and microorganisms were collected periodically to observe the nitrogen removal and anaerobic ammonium nitrogen oxidation of plant microorganisms during continuous culture. In addition, changes in nitrogen gas composition in the effluent gas were monitored daily. Thereafter, the operating conditions were changed according to the degree of removal of ammonia nitrogen and nitrite nitrogen, the composition and the amount of nitrogen gas in the generated gas.

The gas generated from the continuous incubator was measured using a gas meter. The gas content was 1/8 inch diameter, 6 ft long stainless column filled with Porapak Q (80/100), and TCD (Thermal Conductivity). Gas components were analyzed by GC (HP 6890N) equipped with a detector. The generated gas was analyzed under operating conditions of an inlet temperature of 80 ° C, an oven temperature of 70 ° C, and a detector temperature of 200 ° C. Argon gas was used as a carrier gas. Sample analysis of pH, alkalinity, VSS, NH ₃ -N was analyzed according to Standard Methods (APHA, 1998), and NO ₂ -N, NO ₃ -N analysis was performed using ion chromatography (DX-500, DIONEX). .

- result -

The analysis result in the culture apparatus which concerns on an Example and a comparative example is as follows.

The continuous culture apparatus of the example in which the incubator filled with the positively charged polypropylene nonwoven fabric was placed in front of the upflow continuous flow reactor started showing excellent anaerobic ammonium oxidation activity 10 days after the culture. Increasing the concentration of ammonia nitrogen and nitrite nitrogen in the inflow substrate from 70 to 500 mg / L showed excellent activity and showed high nitrogen conversion rate of 1 kg N / m ³ -d within 3 months of culture. .

On the other hand, the culture apparatus of the comparative example was not able to efficiently use ammonia nitrogen as the inflow substrate and showed a low nitrogen conversion rate of less than 0.1 kg N / m ³ -d despite the long-term culture for 3 months. This is because the anaerobic ammonium oxidizing bacteria proliferated and were not easily attached to the granular anaerobic ammonium oxidizing bacteria and were lost to the effluent.

On the other hand, in the comparative example, the VSS concentration in the effluent was found to be between 30 and 100 mg / L, but in the case of the example, the VSS concentration in the effluent was maintained below 10 mg / L, and in the comparative example, depending on the loss of anaerobic ammonium oxide Activity did not increase easily.

The molar ratio of 0.26 (see prior art [Reaction Scheme 5]): 1.32: For general and the formation of nitrate nitrogen as a by-product during the anaerobic ammonium oxidation, the resulting ratio of _{^{NH 4 +: NO 2 -:}} - = 1 NO 3 It is known to follow.

In the present embodiment, under normal conditions, about 0.24 mole of nitrate nitrogen was generated per ammonia nitrogen removed, and the amount of nitrogen gas similar to the theoretical nitrogen gas generation amount based on the removed ammonia nitrogen shown in Equation 1 below. Was confirmed to occur.

Theoretical daily gas generation based on removed NH ₄ ⁺ (mL N ₂ / day, based on temperature 35 ° C) = [{NH ₄ ⁺ removal (mg / L) × flow rate (L / day)} × 1.02N ₂ mM / 1 NH ₄ ⁺ mM × 22.4 mL / mM × (273 + 35) / 273

At the beginning of operation, less than 5% of methane gas was generated by organic matter in anaerobic granular sludge, and less than 0.1% of N ₂ O was generated, but N ₂ O was not detected in the generated gas after one month of continuous culture. Heterotrophic denitrification did not appear to occur.

As such, in the conventional process without a separate culture tank at the front end, anaerobic ammonium oxide bacteria are discharged into the effluent and a culture period of about 1 year or more is required to reach a condition of 1 kg N / m ³ -day. According to the present invention, it is possible to stably remove nitrogen even under a load of 1 kg N / m ³ -day, which is about 2.5 times the existing nitrogen removal process, within three months of significantly increasing the activity.

According to the method and apparatus for continuously culturing anaerobic ammonium oxidizing bacteria of the present invention, the growth time of anaerobic ammonium oxidizing bacteria with extremely slow growth rate can be drastically shortened. Therefore, the initial operation time of the highly concentrated nitrogen-containing wastewater treatment process using anaerobic ammonium oxidizing bacteria can be drastically reduced, so that even if the process is actually applied, the period can be shortened and the stability of the process can be expected. There is no.

Claims (16)

As a continuous culture method of anaerobic ammonium oxide bacteria, Introducing a culture solution into an upflow reactor in which microorganisms having anaerobic ammonium oxide bacteria and anaerobic granular sludge are planted and culturing the anaerobic ammonium oxide bacteria (S1); Culturing anaerobic ammonium oxidizing bacteria by injecting a part or all of the effluent from the reactor of step S1 into the anaerobic ammonium oxidizing bacteria loss prevention culture tank filled with a positively charged nonwoven fabric (S2); And Continuous method of anaerobic ammonium oxidizing bacteria comprising the step (S3) of introducing the effluent from the culture tank of the step S2 back to the reactor of the step S1. The method of claim 1, Method of continuous culture of anaerobic ammonium oxide bacteria characterized in that for using the reactor in which the solid residence time is maintained for 50 days or more as the reactor in the step S1. The method according to claim 1 or 2, Anaerobic ammonium oxide, characterized in that the anaerobic granular sludge of 1 to 3mm in the reactor is planted in 50% or more of the total reactor volume and the microorganism having the activity of anaerobic ammonium oxide bacteria is planted to 10% of the total reactor volume in the step S1. Continuous culture method of bacteria. The method according to claim 1 or 2, Continuous culture method of anaerobic ammonium oxide bacteria characterized in that the flow rate of the effluent of the step S1 is maintained at 50m / hr or more. The method according to claim 1 or 2, The method of continuous culture of anaerobic ammonium oxide bacteria, characterized in that to maintain the redox potential in the reactor to -100 to -150 mV within 30 days of the culture of the anaerobic ammonium oxide bacteria in the step S1. The method of claim 5, The method of continuous culturing anaerobic ammonium oxidizing bacteria, characterized in that to control the redox potential by adding hydrogen sulfide and hydrazine to the reactor in the step S1. The method according to claim 1 or 2, After 30 days of culturing the anaerobic ammonium oxide bacteria in the step S1, the redox potential in the reactor is maintained at -30 to -100mV characterized in that the continuous culture method of anaerobic ammonium oxide bacteria. The method of claim 7, wherein Method of continuously culturing anaerobic ammonium oxidizing bacteria, characterized in that by controlling the molar ratio of ammonia nitrogen / nitrite nitrogen introduced into the reactor in the step S1 to 0.9 to 0.95. As a continuous culture device of anaerobic ammonium oxide bacteria, An upflow reactor in which microorganisms having an activity of anaerobic ammonium oxidizing bacteria and anaerobic granular sludge are seeded, and culture medium is introduced to incubate anaerobic ammonium oxidizing bacteria; And Anaerobic ammonium, characterized in that it comprises a portion or all of the effluent from the reactor, the positively charged non-woven fabric is filled, the discharged effluent flowing into the reactor again anaerobic ammonium oxide loss prevention culture tank; Continuous culture apparatus of oxidizing bacteria. The method of claim 9, Continuous culture apparatus of anaerobic ammonium oxide bacteria characterized in that the solid residence time of the reactor is maintained for 50 days or more. The method according to claim 9 or 10, Anaerobic granular sludge of 1 to 3 mm is planted in the reactor at least 50% of the total reactor volume, and the microorganisms having the activity of anaerobic ammonium oxidizing bacteria are planted to 10% of the total reactor volume. Culture device. The method according to claim 9 or 10, Continuous culture apparatus of anaerobic ammonium oxide bacteria characterized in that the flow rate of the effluent of the reactor is 50m / hr or more. The method according to claim 9 or 10, Continuous culture apparatus of anaerobic ammonium oxidizing bacteria, characterized in that the redox potential in the reactor is maintained at -100 to -150 mV within 30 days of culturing the anaerobic ammonium oxidizing bacteria. The method of claim 13, Hydrogen sulfide and hydrazine is added to the reactor, characterized in that the continuous culture device of anaerobic ammonium oxide bacteria. The method according to claim 9 or 10, After 30 days of culturing the anaerobic ammonium oxidizing bacteria redox potential in the reactor is characterized in that the continuous culture apparatus of the anaerobic ammonium oxidizing bacteria is maintained at -30 to -100mV. The method of claim 15, Continuous culture apparatus of anaerobic ammonium oxide bacteria characterized in that the molar ratio of ammonia nitrogen / nitrite nitrogen to the reactor is 0.9 to 0.95.

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