CN108943518A - A kind of bionical duodenum and preparation method thereof - Google Patents
A kind of bionical duodenum and preparation method thereof Download PDFInfo
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- CN108943518A CN108943518A CN201811143735.4A CN201811143735A CN108943518A CN 108943518 A CN108943518 A CN 108943518A CN 201811143735 A CN201811143735 A CN 201811143735A CN 108943518 A CN108943518 A CN 108943518A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C33/00—Moulds or cores; Details thereof or accessories therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B29C41/00—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor
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- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
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Abstract
The invention discloses a kind of bionical duodenums and preparation method thereof, belong to bionics techniques field and field of biotechnology.Bionical duodenum of the invention is made by being applied in the accurate human body intestinal canal anatomical model duodenum of medicine Gastroenterology dept. after mixing substrate (one of silica gel, latex or hydrogel or a variety of) and auxiliary material (silicone oil and/or curing agent) according to certain mass ratio；Bionical duodenal simulation performance of the invention is superior, and there is people really duodenal form and physiological structure, the duodenal digestive environments of people can really be simulated, such as, this bionical duodenal inside has annular corrugations, enteral surface area can be increased, food is preferably digested and assimilated for microbial flora in duodenum, simultaneously, this bionical duodenal inside has intestinal villi and intestinal villi is hollow structure, intestinal juice can be secreted, the function of duodenal digestion and absorption is greatly reappeared.
Description
Technical field
The present invention relates to a kind of bionical duodenums and preparation method thereof, belong to bionics techniques field and biotechnology neck
Domain.
Background technique
Food, drug research process in, it is objective accurately evaluate food, drug the intracorporal digestion process of people for
It determines food component, pharmaceutical ingredients and they is most important to the effect of human body alimentary canal.
The in-vitro simulated device of people's alimentary canal (such as SHIME, IViDiS, TIM, DGM, HGS) be to the digestive system of people and
Its digestive environments, computational fluid dynamics behavior in the digestive tract carry out the device of human simulation, analog difference sample model in vitro
In the influence to enteric microorganism of digestion process and these samples, so that the research and development for new type functional food provide largely
Valuable data replace completely if predicting live test as " preceding test " or part replace live test, reachable
To the purpose for reducing cost and time, raising repeatability and accuracy, and without theoretical limitation.
Therefore, the in-vitro simulated device of people's alimentary canal is more crucial in the research to food, drug or even microorganism etc.,
Huge convenience is provided for the research of Food Science, human nutriology, is that mouse experiment or human volunteer experiment cannot
Analogy.
And main body of the alimentary canal (mainly including stomach, small intestine, large intestine) as the in-vitro simulated device of people's alimentary canal is simulated, it is right
Really gastral emulator is most important in the simulation performance of the entire in-vitro simulated device of people's alimentary canal by people.
Currently, people make simulation alimentary canal model usually using materials such as silica gel, latexes, digestion can be reappeared well
Digestive environments in road, such as it is inviscid, not soluble in water, do not react with soda acid, high-low temperature resistant.
But the internal physiological structures of these existing alimentary canal models made of silica gel, latex are smooth, single
, for example, small intestine model, these small intestine models are often with there is following fault of construction: first is that not having intestinal villi, contacting enteral
Food area is too small, causes the function to the enteral digestion and absorption of realization limited；Second is that intestinal juice is embodied in the form of directly adding,
Intestinal fluid secretion is uneven, leads to the measurement result inaccuracy of experiment.
Also, these alimentary canal models are usually simply made as overall structure, for example, disposable with silica gel or latex
It is molded into whole stomach, these whole stomaches cannot simulate " returning jet flow " function between antrum and body of stomach, lead to the measurement result of experiment
Inaccuracy.
Meanwhile these alimentary canal models made by silica gel, latex are in simulation performance, such as hardness, expansion rate, elasticity side
Face is actually and not fully up to expectations, and still there are a certain distance with the true alimentary canal of people.
Therefore, it is badly in need of carrying out Bionic digestion road innovation upgrading to promote its simulation performance, promote its simulation real human body
The function at gastral digestive environments (inviscid, not soluble in water, do not react with soda acid, high-low temperature resistant) and each position of alimentary canal
The authenticity of energy.
Summary of the invention
To solve the above problems, the present invention provides a kind of bionical duodenums and preparation method thereof.This bionical 12 finger
Intestines be by by substrate (one of silica gel, latex or hydrogel or a variety of) and auxiliary material (curing agent) according to certain mass ratio
Medicine digestion is applied to after (mass ratio between substrate, silicone oil and curing agent is 100:0.5~1.5:2.5~3.5) mixing
It is made in the accurate human body intestinal canal anatomical model duodenum of section；This bionical duodenal superior (Shao Er A of simulation performance
Hardness be 40 ± 5, expansion rate be 22 ± 5%, elasticity is 250 ± 50%), and there is people really duodenal form and physiology
Structure can really simulate the duodenal digestive environments of people, for example, this bionical duodenal inside has annular wrinkle
Pleat can increase enteral surface area, and then preferably digest and assimilate food for microbial flora in duodenum, meanwhile, this is imitative
It is hollow structure that raw duodenal inside, which has intestinal villi and intestinal villi, can secrete intestinal juice, greatly reappears 12 and refers to
The function of the digestion and absorption of enteral, therefore, this bionical duodenum analog food, drug, microorganism etc. are in digestive system ten
Truth function in two duodenum 12, food, drug research process in have great application prospect.
Technical scheme is as follows:
The present invention provides a kind of bionical duodenal preparation method, the method is that plastic material is put into medicine
It is modeled in the accurate human body duodenum anatomical model of Gastroenterology dept., human body duodenum anatomical model is removed, in plasticity material
Each recess corresponding with intestinal villi on human body duodenum anatomical model of material stabs out duck eye with spicule, by needle
Shape object remains on plastic material and carries out curing molding to plastic material, obtains having inside true duodenum
The duodenum mold of physiological structure；Bionical duodenal substrate will be made according to certain mass ratio and auxiliary material is mixed
It closes, obtains bionical duodenum raw material；Bionical duodenum raw material are applied to duodenum mould according to certain thickness
Has the one side being in contact with human body duodenum anatomical model, standing, which makes to be applied to bionical the 12 of duodenum die surface, refers to
Intestines raw material coagulation forming extracts spicule and simultaneously sloughs duodenum mold, obtains having intestinal villi and is hollow bionical
Duodenum；
Or the method is that plastic material is put into the accurate human body duodenum anatomical model of medicine Gastroenterology dept. to carry out
Modeling, remove human body duodenum anatomical model, plastic material each with it is small on human body duodenum anatomical model
The corresponding recess of intestinal villus stabs out duck eye with spicule, and spicule is remained on plastic material and to plasticity
Material carries out curing molding, obtains the duodenum mold with true duodenum internal physiological structures；According to certain matter
Amount is mixed than that will make bionical duodenal substrate and auxiliary material, obtains bionical duodenum raw material；According to certain
Bionical duodenum raw material are applied to the one side that duodenum mold is in contact with human body duodenum anatomical model by thickness,
The bionical duodenum raw material coagulation forming for making to be applied to duodenum die surface is stood, spicule is only extracted, is had
There is intestinal villi and is hollow bionical duodenum；In the side that bionical duodenum is not in contact with duodenum mold
Bionical duodenum raw material are applied to spacer according to certain thickness and not referred to bionical 12 by one layer of spacer of upper covering
In the side that intestines are in contact, stand the bionical duodenum raw material coagulation forming for making to be applied to separator surface, removal every
From object and duodenum mold is sloughed, is obtained with duodenum physiological structure villus, and bionical the 12 of intestinal juice can be secreted and referred to
Intestines；
The substrate includes one of silica gel, latex or hydrogel or a variety of；The auxiliary material includes silicone oil and/or solidification
Agent.
In one embodiment of the invention, the accurate human body intestinal canal anatomical model of the medicine Gastroenterology dept. is true enteron aisle
1:1 hollow mold.
In one embodiment of the invention, the plastic material includes clay, paraffin, glass or steel.
In one embodiment of the invention, the plastic material includes clay, paraffin, glass or steel.
In one embodiment of the invention, the clay is soft ceramic mud.
In one embodiment of the invention, the method is that soft ceramic mud is put into the accurate human body 12 of medicine Gastroenterology dept.
It is modeled in duodenum 12 anatomical model, removes human body duodenum anatomical model, each in soft ceramic mud refers to human body 12
The corresponding recess of intestinal villi stabs out duck eye with spicule on intestines anatomical model, and spicule is remained on soft ceramic mud
And soft ceramic mud is subjected to baking makes its curing molding, natural cooling generates crack to prevent mold to be quickly cooled down, and obtains having true
The duodenum mold of real duodenum internal physiological structures；Bionical duodenal substrate will be made according to certain mass ratio
It is put into the row of vacuumizing in vacuum machine after being mixed with auxiliary material to steep, obtains bionical duodenum raw material；According to certain thickness
Bionical duodenum raw material are applied to the inside of duodenum mold, standing makes to be applied to the imitative of duodenum die surface
Raw duodenum raw material coagulation forming extracts needle and simultaneously sloughs duodenum mold, obtains having intestinal villi and is hollow
Bionical duodenum；
Or the method is that first soft ceramic mud is put into the accurate human body duodenum anatomical model of medicine Gastroenterology dept. to build
Mould removes human body duodenum anatomical model, in each and intestinal villi on human body duodenum anatomical model of soft ceramic mud
Corresponding recess stabs out duck eye with spicule, and remaining on soft ceramic mud and soft ceramic mud is carried out baking spicule makes
Its curing molding, natural cooling generate crack to prevent mold to be quickly cooled down, obtain having true duodenum internal physiological knot
The duodenum mold of structure；According to certain mass ratio will make bionical duodenal substrate and auxiliary material mixed after be put into
The row of vacuumizing steeps in vacuum machine, obtains bionical duodenum raw material；According to certain thickness by bionical duodenum raw material
It is applied to the inside of duodenum mold, stands the bionical duodenum raw material solidification for making to be applied to duodenum die surface
Molding, only extracts needle, obtains having intestinal villi and for hollow bionical duodenum；Do not refer to 12 in bionical duodenum
In the side that intestines mold is in contact cover one layer of spacer, according to certain thickness by bubble-free mixed raw material be applied to every
In the side not being in contact with bionical duodenum from object, the bionical duodenum former material for making to be applied to separator surface is stood
Expect coagulation forming, remove spacer and slough duodenum mold, obtains with duodenum physiological structure villus, and can secrete
The bionical duodenum of intestinal juice.
In one embodiment of the invention, the roasted time is 15~30min.
In one embodiment of the invention, the diameter of the duck eye is 0.2~1mm
In one embodiment of the invention, each in soft ceramic mud on human body duodenum anatomical model
When the corresponding recess of intestinal villi stabs out duck eye, spicule should be made to penetrate soft ceramic mud and make one end of spicule beyond soft pottery
The side at least 2mm that mud is in contact with human body duodenum anatomical model.
In one embodiment of the invention, the diameter of the duodenum mold is 3~4cm, 20~30cm of length.
In one embodiment of the invention, the mass ratio between the substrate, silicone oil and curing agent is 100:
(0.5~1.5): (2.5~3.5).
In one embodiment of the invention, the mass ratio between the substrate and auxiliary material is 100:1:3.
In one embodiment of the invention, the substrate is silica gel.
In one embodiment of the invention, the silica gel is transparent silica gel or translucent silica gel.
In one embodiment of the invention, the curing agent includes ethyl orthosilicate, vinyl triamine, DETA ammonia second
One of base piperazine AE, m-phenylene diamine (MPD) m-PDAMPD, diaminodiphenyl-methane DDM or HT-972DEH-50 or a variety of.
In one embodiment of the invention, the condition of the row of vacuumizing bubble be vacuum machine negative pressure -0.1~
0.09MPa, 5~10min of time.
In one embodiment of the invention, the condition of the row's of vacuumizing bubble is vacuum machine negative pressure 0.09MPa, time
7min。
In one embodiment of the invention, the bionical duodenum raw material are applied to duodenum die surface
With a thickness of 1~2mm.
In one embodiment of the invention, the bionical duodenum raw material are applied to duodenum die surface
With a thickness of 1.5mm.
In one embodiment of the invention, the time of the standing is 2~4h.
In one embodiment of the invention, the time of the standing is 3h.
In one embodiment of the invention, the spacer includes oiliness paper or preservative film.
In one embodiment of the invention, the bionical duodenum raw material are applied to separator surface surface
With a thickness of 1~5mm.
In one embodiment of the invention, the bionical duodenum raw material are applied to separator surface surface
With a thickness of 1.5mm.
The present invention provides the bionical duodenums being prepared using the bionical duodenal preparation method of above-mentioned one kind.
The present invention provides the bionical duodenal preparation methods of above-mentioned one kind or above-mentioned bionical 12 be prepared to refer to
Application of the intestines in the simulation practical digestive system of human body.
The utility model has the advantages that
(1) bionical duodenum simulation performance of the invention it is superior (shore a hardness be 40 ± 5, expansion rate be 22 ± 5%,
Elasticity is 250 ± 50%), there is people really duodenal form and physiological structure, can really simulate in people's duodenum
Digestive environments；
(2) bionical duodenal inside of the invention has annular corrugations, can increase enteral surface area, and then for ten
Two finger gut microfloras preferably digest and assimilate food；
(3) bionical duodenal inside of the invention, which has intestinal villi and intestinal villi, to be double-deck hollow knot
Structure greatly reappears ten if intestinal juice, which is filled to hollow space, can make intestinal villi secrete intestinal juice automatically during wriggling
The function of digestion and absorption in two duodenum 12；
(4) bionical duodenum of the invention can be dynamically transparent or translucent, be able to achieve true and real-time 12
The observation in vitro of Digestive States inside duodenum 12；
(5) bionical duodenum of the invention has inviscid, not soluble in water, not anti-with soda acid using silica gel as substrate
It answers, the advantages such as high-low temperature resistant, duodenal digestion and absorption environment can be reappeared well；
(6) bionical duodenum analog food of the invention, drug, microorganism etc. are in digestive system duodenum
Truth function, food, drug research process in have great application prospect.
Detailed description of the invention
Fig. 1: the bionical duodenal full face of the present invention；
Fig. 2: the bionical duodenal reverse side photo of the present invention；
Fig. 3: the bionical duodenal intestinal villi photo of the present invention；
Fig. 4: the bionical duodenal intestinal villi photo of the present invention.
Specific embodiment
The present invention will be further elaborated combined with specific embodiments below.
Detection method involved in following embodiments is as follows:
Intestinal surface accumulates detection method:
S=2 π r1h1+n(π-1)r2h2；
Wherein, S is intestinal surface product, r1For intestines radius, h1For intestines height, n is intestinal wall quantity, r2For intestinal wall radius, h2For intestinal wall
It is high.
Transparency detection method:
Using range estimation, whether observation silica gel is transparent, bright, has bubble, and stretch silica gel, sees whether whiten, whiten
Be it is opaque, the foods such as rice, corn are put into silica gel inside, the appearance that whether can clearly see food seen.
If the surface of food can be clearly apparent, transparency is good；If it can be seen that the profile of food, transparency are moderate；If
It can't see the image of food, then poor transparency.
Hardness (Shao Er hardness) detection method:
It is measured with Shao Er hardometer, the surface of stomach is pressed in scleroscopic pressing, measurement pressing indentation stomach surface
Depth.
Expansion rate detection method:
Pe=(W*m/A) × 100%；
Wherein, Pe is expansion rate, and W is overall balance loading, and A is silica gel area, and m is the lever ratio of pressurized equipment.
Elastomeric check method:
Extension test is carried out to silica gel on tensile testing machine, measures silica gel axial elongation and transverse extension amount, until
Until silica gel is broken.
C=(D-d) × 100%/d；
Wherein, C is spring rate, and D is elongation, and d is silica gel just long amount.
Villus secretes intestinal juice Function detection:
Bionical duodenum is fixed on enteron aisle by the flange-interface of pipeline and visualizes in bionical reactor that (enteron aisle is visual
Change bionical reactor referring to the patent of invention of Publication No. CN108318625A), 100ml pancreatic juice is added and refers to silica gel bionical 12
In intestines, reactor 10min, the volume of observation secretion pancreatic juice are run.
Embodiment 1: bionical duodenal preparation
(1) prepare pottery mud: by soft ceramic mud kneading repeatedly, obtaining the preferable soft ceramic mud of ductility；
(2) duodenum mold is suppressed: the soft ceramic mud mixed being carried out to be put into the accurate human body duodenum of medicine Gastroenterology dept.
It in anatomical model, firmly squeezes, removes human body duodenum anatomical model, obtain that there are true duodenum internal physiological structures
Soft ceramic mud duodenum mold, mould diameter 3cm, length 25cm；
(3) hollow villus production: taking diameter is that 0.5mm needle is several, is pricked at each marking on above-mentioned mold, and stamp is straight
Diameter 1mm, the duck eye of deep 5mm, needle remain in mould inside
(4) duodenum mold baking molding: duodenum mold is put into 140 DEG C of oven and is baked 15 minutes, makes 12
The molding of duodenum 12 mold cured, natural cooling obtain bionical duodenum mold；
(8) prepare silica gel: will be placed in a beaker, fill by the transparent silica gel of 100:1:3, silicone oil and curing agent ethyl orthosilicate
Divide stirring, is uniformly mixed；
(9) uniformly mixed silica gel is put into vacuum machine, negative pressure 0.09MPa, the row's of vacuumizing bubble the time 5 minutes, obtains
Bubble-free silica gel；
(10) bionical duodenum die surface obtained above is uniformly smeared into bubble-free silica gel, thickness about 1mm；
(11) uniform duodenum mold will be smeared and stand 2 hours, after making its thorough coagulation forming, by the needle outside mold
It pulls out, obtains having intestinal villi and for hollow duodenum silica gel model；
(12) needle is only pulled out by above-mentioned, the duodenum die surface for not falling off silica gel covers an oiliness paper, and outside is put
Setting a diameter is 2cm, and the hollow cylinder of high 4cm is fixed to glue；
(13) gap between the oiliness paper and cylindrical body of duodenum mold is uniformly smeared to the silica gel of bubble-free, is stood
2 hours, to its thorough coagulation forming；
(14) silica gel mould solidified is placed in water 10 minutes, after water by oiliness paper softening after, by oiliness paper from
It is taken out in hollow cylinder；
(15) it after silica gel being sloughed duodenum mold, using certain pruning modes, can obtain with duodenum
Physiological structure intestinal wall gauffer and villus, and villus can secrete the bionical duodenum silica gel model of intestinal juice.
Embodiment 2: bionical duodenal preparation
(1) prepare pottery mud: by soft ceramic mud kneading repeatedly, obtaining the preferable soft ceramic mud of ductility；
(2) duodenum mold is suppressed: the soft ceramic mud mixed being carried out to be put into the accurate human body duodenum of medicine Gastroenterology dept.
It in anatomical model, firmly squeezes, removes human body duodenum anatomical model, obtain that there are true duodenum internal physiological structures
Soft ceramic mud duodenum mold, mould diameter 3.5cm, length 25cm；
(3) hollow villus production: taking diameter is that 0.3mm needle is several, is pricked at each marking on above-mentioned mold, and stamp is straight
Diameter 0.5mm, the duck eye of deep 5mm, needle remain in mould inside
(4) duodenum mold baking molding: duodenum mold is put into 150 DEG C of oven and is baked 20 minutes, makes 12
The molding of duodenum 12 mold cured, natural cooling obtain bionical duodenum mold；
(8) prepare silica gel: will be placed in a beaker by the transparent silica gel of 100:1.5:3, silicone oil and curing agent vinyl triamine,
It is sufficiently stirred, is uniformly mixed；
(9) uniformly mixed silica gel is put into vacuum machine, negative pressure 0.09MPa, the row's of vacuumizing bubble the time 5 minutes, obtains
Bubble-free silica gel；
(10) bionical duodenum die surface obtained above is uniformly smeared into bubble-free silica gel, thickness about 1.5mm；
(11) uniform duodenum mold will be smeared and stand 4 hours, after making its thorough coagulation forming, by the needle outside mold
It pulls out, obtains having intestinal villi and for hollow duodenum silica gel model；
(12) needle is only pulled out by above-mentioned, the duodenum die surface for not falling off silica gel covers an oiliness paper, and outside is put
Setting a diameter is 2cm, and the hollow cylinder of high 4cm is fixed to glue；
(13) gap between the oiliness paper and cylindrical body of duodenum mold is uniformly smeared to the silica gel of bubble-free, is stood
1 hour, to its thorough coagulation forming；
(14) silica gel mould solidified is placed in water 10 minutes, after water by oiliness paper softening after, by oiliness paper from
It is taken out in hollow cylinder；
(15) it after silica gel being sloughed duodenum mold, using certain pruning modes, can obtain with duodenum
Physiological structure intestinal wall gauffer and villus, and villus can secrete the bionical duodenum silica gel model of intestinal juice.
Embodiment 3: bionical duodenal preparation
(1) prepare pottery mud: by soft ceramic mud kneading repeatedly, obtaining the preferable soft ceramic mud of ductility；
(2) duodenum mold is suppressed: the soft ceramic mud mixed being carried out to be put into the accurate human body duodenum of medicine Gastroenterology dept.
It in anatomical model, firmly squeezes, removes human body duodenum anatomical model, obtain that there are true duodenum internal physiological structures
Soft ceramic mud duodenum mold, mould diameter 4cm, length 30cm；
(3) hollow villus production: taking diameter is that 0.1mm needle is several, is pricked at each marking on above-mentioned mold, and stamp is straight
Diameter 0.2mm, the duck eye of deep 5mm, needle remain in mould inside
(4) duodenum mold baking molding: duodenum mold is put into 130 DEG C of oven and is baked 30 minutes, makes 12
The molding of duodenum 12 mold cured, natural cooling obtain bionical duodenum mold；
(8) prepare silica gel: will be set by the transparent silica gel of 100:1:2.5, silicone oil and curing agent diaminodiphenyl-methane DDM
It in beaker, is sufficiently stirred, is uniformly mixed；
(9) uniformly mixed silica gel is put into vacuum machine, negative pressure 0.09MPa, the row's of vacuumizing bubble the time 5 minutes, obtains
Bubble-free silica gel；
(10) bionical duodenum die surface obtained above is uniformly smeared into bubble-free silica gel, thickness about 2mm；
(11) uniform duodenum mold will be smeared and stand 1 hour, after making its thorough coagulation forming, by the needle outside mold
It pulls out, obtains having intestinal villi and for hollow duodenum silica gel model；
(12) needle is only pulled out by above-mentioned, the duodenum die surface for not falling off silica gel covers an oiliness paper, and outside is put
Setting a diameter is 2cm, and the hollow cylinder of high 4cm is fixed to glue；
(13) gap between the oiliness paper and cylindrical body of duodenum mold is uniformly smeared to the silica gel of bubble-free, is stood
1 hour, to its thorough coagulation forming；
(14) silica gel mould solidified is placed in water 10 minutes, after water by oiliness paper softening after, by oiliness paper from
It is taken out in hollow cylinder；
(15) it after silica gel being sloughed duodenum mold, using certain pruning modes, can obtain with duodenum
Physiological structure intestinal wall gauffer and villus, and villus can secrete the bionical duodenum silica gel model of intestinal juice.
Comparative example 1: bionical duodenal preparation (no villus)
(1) prepare pottery mud: by soft ceramic mud kneading repeatedly, obtaining the preferable soft ceramic mud of ductility；
(2) duodenum mold is suppressed: the soft ceramic mud mixed being carried out to be put into the accurate human body duodenum of medicine Gastroenterology dept.
It in anatomical model, firmly squeezes, removes human body duodenum anatomical model, obtain that there are true duodenum internal physiological structures
Soft ceramic mud duodenum mold, mould diameter 3cm, length 30cm；
(4) duodenum mold baking molding: duodenum mold is put into 130 DEG C of oven and is baked 30 minutes, makes 12
The molding of duodenum 12 mold cured, natural cooling obtain bionical duodenum mold；
(8) prepare silica gel: will be placed in a beaker, fill by the transparent silica gel of 100:1:3, silicone oil and curing agent ethyl orthosilicate
Divide stirring, is uniformly mixed；
(9) uniformly mixed silica gel is put into vacuum machine, negative pressure 0.09MPa, the row's of vacuumizing bubble the time 5 minutes, obtains
Bubble-free silica gel；
(10) bionical duodenum die surface obtained above is uniformly smeared into bubble-free silica gel, thickness about 1.5mm；
(11) uniform duodenum mold will be smeared and stand 1 hour, make its thorough coagulation forming；
(15) it after silica gel being sloughed duodenum mold, using certain pruning modes, can obtain with duodenum
The bionical duodenum silica gel model of physiological structure intestinal wall gauffer.
Comparative example 2: bionical duodenal preparation (silica gel does not vacuumize after mixing)
(1) prepare pottery mud: by soft ceramic mud kneading repeatedly, obtaining the preferable soft ceramic mud of ductility；
(2) duodenum mold is suppressed: the soft ceramic mud mixed being carried out to be put into the accurate human body duodenum of medicine Gastroenterology dept.
It in anatomical model, firmly squeezes, removes human body duodenum anatomical model, obtain that there are true duodenum internal physiological structures
Soft ceramic mud duodenum mold, mould diameter 3.5cm, length 30cm；
(3) hollow villus production: taking diameter is that 0.2mm needle is several, is pricked at each marking on above-mentioned mold, and stamp is straight
Diameter 0.5mm, the duck eye of deep 5mm, needle remain in mould inside
(4) duodenum mold baking molding: duodenum mold is put into 140 DEG C of oven and is baked 20 minutes, makes 12
The molding of duodenum 12 mold cured, natural cooling obtain bionical duodenum mold；
(8) prepare silica gel: beaker will be placed in by the transparent silica gel of 100:0.5:2.5, silicone oil and curing agent ethyl orthosilicate
In, it is sufficiently stirred, is uniformly mixed；
(9) bionical duodenum die surface obtained above is uniformly smeared into silica gel, thickness about 1.5mm；
(10) uniform duodenum mold will be smeared and stand 2 hours, after making its thorough coagulation forming, by the needle outside mold
It pulls out, obtains having intestinal villi and for hollow duodenum silica gel model；
(11) needle is only pulled out by above-mentioned, the duodenum die surface for not falling off silica gel covers an oiliness paper, and outside is put
Setting a diameter is 2cm, and the hollow cylinder of high 4cm is fixed to glue；
(12) gap between the oiliness paper and cylindrical body of duodenum mold is uniformly smeared to the silica gel of bubble-free, is stood
2 hours, to its thorough coagulation forming；
(13) silica gel mould solidified is placed in water 10 minutes, after water by oiliness paper softening after, by oiliness paper from
It is taken out in hollow cylinder；
(14) it after silica gel being sloughed duodenum mold, using certain pruning modes, can obtain with duodenum
Physiological structure intestinal wall gauffer and villus, and villus can secrete the bionical duodenum silica gel model of intestinal juice.
Comparative example 3: bionical duodenal preparation (smearing silica gel thickness to increase)
(1) prepare pottery mud: by soft ceramic mud kneading repeatedly, obtaining the preferable soft ceramic mud of ductility；
(2) duodenum mold is suppressed: the soft ceramic mud mixed being carried out to be put into the accurate human body duodenum of medicine Gastroenterology dept.
It in anatomical model, firmly squeezes, removes human body duodenum anatomical model, obtain that there are true duodenum internal physiological structures
Soft ceramic mud duodenum mold, mould diameter 4cm, length 30cm；
(3) hollow villus production: taking diameter is that 0.4mm needle is several, is pricked at each marking on above-mentioned mold, and stamp is straight
Diameter 0.6mm, the duck eye of deep 5mm, needle remain in mould inside
(4) duodenum mold baking molding: duodenum mold is put into 130 DEG C of oven and is baked 30 minutes, makes 12
The molding of duodenum 12 mold cured, natural cooling obtain bionical duodenum mold；
(8) prepare silica gel: beaker will be placed in by the transparent silica gel of 100:1.5:3.5, silicone oil and curing agent ethyl orthosilicate
In, it is sufficiently stirred, is uniformly mixed；
(9) uniformly mixed silica gel is put into vacuum machine, negative pressure 0.09MPa, the row's of vacuumizing bubble the time 5 minutes, obtains
Bubble-free silica gel；
(10) bionical duodenum die surface obtained above is uniformly smeared into bubble-free silica gel, thickness about 6mm；
(11) uniform duodenum mold will be smeared and stand 2 hours, after making its thorough coagulation forming, by the needle outside mold
It pulls out, obtains having intestinal villi and for hollow duodenum silica gel model；
(12) needle is only pulled out by above-mentioned, the duodenum die surface for not falling off silica gel covers an oiliness paper, and outside is put
Setting a diameter is 2cm, and the hollow cylinder of high 4cm is fixed to glue；
(13) gap between the oiliness paper and cylindrical body of duodenum mold is uniformly smeared to the silica gel of bubble-free, is stood
2 hours, to its thorough coagulation forming；
(14) silica gel mould solidified is placed in water 10 minutes, after water is by the softening of oiliness paper, by oiliness paper from sky
It is taken out in heart cylindrical body；
(15) it after silica gel being sloughed duodenum mold, using certain pruning modes, can obtain with duodenum
Physiological structure intestinal wall gauffer and villus, and villus can secrete the bionical duodenum silica gel model of intestinal juice.
Comparative example 4: bionical duodenal preparation (not adding silicone oil)
(1) prepare pottery mud: by soft ceramic mud kneading repeatedly, obtaining the preferable soft ceramic mud of ductility；
(2) duodenum mold is suppressed: the soft ceramic mud mixed being carried out to be put into the accurate human body duodenum of medicine Gastroenterology dept.
It in anatomical model, firmly squeezes, removes human body duodenum anatomical model, obtain that there are true duodenum internal physiological structures
Soft ceramic mud duodenum mold, mould diameter 4cm, length 30cm；
(3) hollow villus production: taking diameter is that 0.4mm needle is several, is pricked at each marking on above-mentioned mold, and stamp is straight
Diameter 0.6mm, the duck eye of deep 5mm, needle remain in mould inside
(4) duodenum mold baking molding: duodenum mold is put into 130 DEG C of oven and is baked 30 minutes, makes 12
The molding of duodenum 12 mold cured, natural cooling obtain bionical duodenum mold；
(8) prepare silica gel: will be placed in a beaker, be sufficiently stirred by the transparent silica gel of 100:3 and curing agent ethyl orthosilicate,
It is uniformly mixed；
(9) uniformly mixed silica gel is put into vacuum machine, negative pressure 0.09MPa, the row's of vacuumizing bubble the time 5 minutes, obtains
Bubble-free silica gel；
(10) bionical duodenum die surface obtained above is uniformly smeared into bubble-free silica gel, thickness about 2mm；
(11) uniform duodenum mold will be smeared and stand 2 hours, after making its thorough coagulation forming, by the needle outside mold
It pulls out, obtains having intestinal villi and for hollow duodenum silica gel model；
(12) needle is only pulled out by above-mentioned, the duodenum die surface for not falling off silica gel covers an oiliness paper, and outside is put
Setting a diameter is 2cm, and the hollow cylinder of high 4cm is fixed to glue；
(13) gap between the oiliness paper and cylindrical body of duodenum mold is uniformly smeared to the silica gel of bubble-free, is stood
2 hours, to its thorough coagulation forming；
(14) silica gel mould solidified is placed in water 10 minutes, after water is by the softening of oiliness paper, by oiliness paper from sky
It is taken out in heart cylindrical body；
(15) it after silica gel being sloughed duodenum mold, using certain pruning modes, can obtain with duodenum
Physiological structure intestinal wall gauffer and villus, and villus can secrete the bionical duodenum silica gel model of intestinal juice.
Comparative example 5: bionical duodenal preparation (addition glycerol)
(1) prepare pottery mud: by soft ceramic mud kneading repeatedly, obtaining the preferable soft ceramic mud of ductility；
(2) duodenum mold is suppressed: the soft ceramic mud mixed being carried out to be put into the accurate human body duodenum of medicine Gastroenterology dept.
It in anatomical model, firmly squeezes, removes human body duodenum anatomical model, obtain that there are true duodenum internal physiological structures
Soft ceramic mud duodenum mold, mould diameter 4cm, length 30cm；
(3) hollow villus production: taking diameter is that 0.4mm needle is several, is pricked at each marking on above-mentioned mold, and stamp is straight
Diameter 0.6mm, the duck eye of deep 5mm, needle remain in mould inside
(4) duodenum mold baking molding: duodenum mold is put into 130 DEG C of oven and is baked 30 minutes, makes 12
The molding of duodenum 12 mold cured, natural cooling obtain bionical duodenum mold；
(8) prepare silica gel: will be placed in a beaker, fill by the transparent silica gel of 100:1:3, glycerol and curing agent ethyl orthosilicate
Divide stirring, is uniformly mixed；
(9) uniformly mixed silica gel is put into vacuum machine, negative pressure 0.09MPa, the row's of vacuumizing bubble the time 5 minutes, obtains
Bubble-free silica gel；
(10) bionical duodenum die surface obtained above is uniformly smeared into bubble-free silica gel, thickness about 2mm；
(11) uniform duodenum mold will be smeared and stand 2 hours, after making its thorough coagulation forming, by the needle outside mold
It pulls out, obtains having intestinal villi and for hollow duodenum silica gel model；
(12) needle is only pulled out by above-mentioned, the duodenum die surface for not falling off silica gel covers an oiliness paper, and outside is put
Setting a diameter is 2cm, and the hollow cylinder of high 4cm is fixed to glue；
(13) gap between the oiliness paper and cylindrical body of duodenum mold is uniformly smeared to the silica gel of bubble-free, is stood
2 hours, to its thorough coagulation forming；
(14) silica gel mould solidified is placed in water 10 minutes, after water is by the softening of oiliness paper, by oiliness paper from sky
It is taken out in heart cylindrical body；
(15) it after silica gel being sloughed duodenum mold, using certain pruning modes, can obtain with duodenum
Physiological structure intestinal wall gauffer and villus, and villus can secrete the bionical duodenum silica gel model of intestinal juice.
Embodiment 4: bionical duodenal detection
The bionical duodenum that takes human body duodenum anatomical model, the embodiment 1-3 of same length to be prepared, comparative example
Bionical duodenum and same kind of products at abroad (8 model of Fast purchased from Dragon Skin company of the U.S. that 1-5 is prepared
The bionical duodenum of silica gel) carry out intestinal surface product, transparency, hardness, expansion rate, elasticity and villus Function detection, detection is tied
Fruit such as table 1.
As shown in Table 1, bionical duodenum of the invention is closer to true duodenal index comparison, with foreign countries
Similar product comparison, index is more excellent, therefore, the alternative true duodenum of bionical duodenum of the invention come predict living body try
It tests, replaces completely or part replaces live test, achieve the purpose that reduce cost and time, improve repeatability and accuracy, and
And not theoretical limitation.
1 performance test results of table
Although the present invention has been described by way of example and in terms of the preferred embodiments, it is not intended to limit the invention, any to be familiar with this skill
The people of art can do various change and modification, therefore protection model of the invention without departing from the spirit and scope of the present invention
Enclosing subject to the definition of the claims.
Claims (10)
1. a kind of bionical duodenal preparation method, which is characterized in that the method is that plastic material is put into medicine to disappear
It is modeled in the accurate human body duodenum anatomical model of change section, human body duodenum anatomical model is removed, in plastic material
Each recess corresponding with intestinal villi on human body duodenum anatomical model stab out duck eye with spicule, will be needle-shaped
Object remains on plastic material and carries out curing molding to plastic material, obtains having raw inside true duodenum
Manage the duodenum mold of structure；Bionical duodenal substrate will be made according to certain mass ratio and auxiliary material mixes,
Obtain bionical duodenum raw material；According to certain thickness by bionical duodenum raw material be applied to duodenum mold with
The one side that human body duodenum anatomical model is in contact stands the bionical duodenum original for making to be applied to duodenum die surface
Material solidification molding extracts spicule and simultaneously sloughs duodenum mold, obtains having intestinal villi and is hollow bionical 12
Duodenum 12；
Or the method is that plastic material is put into the accurate human body duodenum anatomical model of medicine Gastroenterology dept. to model,
Human body duodenum anatomical model is removed, in each and intestinal villi on human body duodenum anatomical model of plastic material
Corresponding recess stabs out duck eye with spicule, by spicule remain on plastic material and to plastic material into
Row curing molding obtains the duodenum mold with true duodenum internal physiological structures；It will according to certain mass ratio
It makes bionical duodenal substrate and auxiliary material is mixed, obtain bionical duodenum raw material；It will according to certain thickness
Bionical duodenum raw material are applied to the one side that duodenum mold is in contact with human body duodenum anatomical model, and standing makes
It is applied to the bionical duodenum raw material coagulation forming of duodenum die surface, spicule is only extracted, obtains with small intestine
Villus and be hollow bionical duodenum；It is covered in the side that bionical duodenum is not in contact with duodenum mold
Bionical duodenum raw material are applied to spacer according to certain thickness and not connected with bionical duodenum by one layer of spacer
In the side of touching, the bionical duodenum raw material coagulation forming for making to be applied to separator surface is stood, removal spacer is simultaneously
Duodenum mold is sloughed, is obtained with duodenum physiological structure villus, and the bionical duodenum of intestinal juice can be secreted；
The substrate includes one of silica gel, latex or hydrogel or a variety of；The auxiliary material includes silicone oil and/or curing agent.
2. a kind of bionical duodenal preparation method as described in claim 1, which is characterized in that the plastic material packet
Containing clay, paraffin, glass or steel.
3. bionical duodenal preparation method as claimed in claim 1 or 2 a kind of, which is characterized in that the duck eye it is straight
Diameter is 0.2~1mm.
4. the bionical duodenal preparation method of one kind a method according to any one of claims 1-3, which is characterized in that described plastic
When each recess corresponding with intestinal villi on human body duodenum anatomical model of property material stabs out duck eye, needle should be made
Shape object penetrates plastic material and one end of spicule is made to be in contact beyond plastic material with human body duodenum anatomical model
The side at least 2mm.
5. the bionical duodenal preparation method of one kind as described in claim 1-4 is any, which is characterized in that the substrate,
Mass ratio between silicone oil and curing agent is 100:(0.5~1.5): (2.5~3.5).
6. the bionical duodenal preparation method of one kind a method as claimed in any one of claims 1 to 5, which is characterized in that described bionical ten
Two duodenum 12 raw material just can be applied after need to carrying out the row's of vacuumizing bubble to duodenum mold；The condition of the row's of vacuumizing bubble
For -0.1~0.09MPa, 5~10min of time.
7. the bionical duodenal preparation method of one kind as described in claim 1-6 is any, which is characterized in that described bionical ten
Two duodenum 12 raw material be applied to duodenum die surface with a thickness of 1~2mm.
8. the bionical duodenal preparation method of one kind as claimed in claim 1, which is characterized in that the standing
Time is 2~4h.
9. bionical 12 finger that any bionical duodenal preparation method of one kind of application claim 1-8 is prepared
Intestines.
10. any bionical duodenal preparation method of one kind of claim 1-8 as claimed in claim 9 is prepared into
To bionical duodenum simulation the practical digestive system of human body in application.
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| PCT/CN2018/117135 WO2020062521A1 (en) | 2018-09-29 | 2018-11-23 | Bionic digestive tract and preparation method and application for bionic digestive tract |
| US16/875,106 US11721241B2 (en) | 2018-09-29 | 2020-05-15 | Bionic digestive tract as well as preparation method and application thereof |
| US18/325,338 US20230306875A1 (en) | 2018-09-29 | 2023-05-30 | Bionic Digestive Tract as well as Preparation Method and Application Thereof |
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| CN103706124A (en) * | 2012-10-06 | 2014-04-09 | 南京大五教育科技有限公司 | Bionic goldfish manufacturing process |
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