CN108931629A - The situation in centrifugal blood is determined using measurement pressure - Google Patents
The situation in centrifugal blood is determined using measurement pressure Download PDFInfo
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- CN108931629A CN108931629A CN201810560634.0A CN201810560634A CN108931629A CN 108931629 A CN108931629 A CN 108931629A CN 201810560634 A CN201810560634 A CN 201810560634A CN 108931629 A CN108931629 A CN 108931629A
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Abstract
The invention discloses a kind of method for determining blood sample situation, the method includes：Blood sample is provided；The metering probe with pump is provided, the pump is for drawing and distributing；The metering probe is inserted into the blood sample with selected distance；The pressure of the measurement during sample is drawn or sample distributes between the sample and pump；The pressure measured is compared with reference value；And issue the signal presence or absence of the situation.The invention discloses a kind of for confirming or detecting in centrifugation of blood samples with the presence or absence of the method for selected blood constitutent layer, the method includes：Measure the pressure that doubtful selected layer in probe is measured during absorption or distribution；The pressure measured is compared with reference value, wherein can confirm the selected layer of the blood constitutent if the pressure and the reference value that measure are substantially the same.In a preferred embodiment, the reference value is the pressure limit of pre-selection.
Description
The application be on 2 7th, 2012 the applying date, it is entitled " using surveying application No. is 201210033058.7
Constant-pressure determines the situation in centrifugal blood " patent of invention divisional application.
The cross reference of related application
Present patent application be filed in the U.S. Provisional Application No.61/439,947 of 2 months 2011 7 mesh non-transitory it is special
Benefit application, the disclosure of which, which is incorporated by reference, to be incorporated herein.
Background technique
The present invention relates to the measurement pressure used in absorption or assigning process to determine the situation in centrifugal blood.Specifically
It says, the present invention relates to detections or confirmation, and packed red cells are drawn from centrifugal blood.
It mostly donates blood and is divided into and (being separated into) following components：Red blood cell, blood platelet, coagulation factor, blood plasma, antibody (are exempted from
Epidemic disease globulin) and leucocyte.As the case may be, people may only receive the cell in blood, only receive the blood coagulation in blood
The factor, or only receive some other blood constitutents.Specific treatment can be realized by being only transfused selected blood constitutent, reduced and caused secondary make
Risk can also efficiently use the different component from same unit blood and treat several patients.
Packed red cells (PRBC) are most common blood transfusion components, it can restore the oxygen carrying capacity of blood.The component can
To be supplied to the patient of blood loss or severe anemia.By the fluid components of red blood cell and blood (blood plasma), and with other cells and
The separation of class cellular component.Red blood cell is concentrated the step, so that their occupied spaces are smaller, because referred to herein as term " is pressed
Product ".Red blood cell can refrigerate most 42 days.Under special circumstances, rare blood type is for example, saved, red blood cell can freeze at most
10 years.Therefore, ability PRBC separated with the other components for being used to transfuse blood is extremely important.
Immunohematology technical field is the science about antigen and antibody, because they are related to transfusing blood to donor and transfusing blood
The management of service.Immunohematological application includes defining blood group, and the identification gestational period may cause incompatible blood transfusion and shifting
The undesirable antibody of plant or complication.Receptor (patient) and donor blood are detected to ensure transfusion safety.The typing of blood
It can manually perform, or (such as Ortho-Clinical Diagnostics, Inc. are sold in automatically or semi-automatically system
Ortho ) in execute.
In immunohematology and clinical chemistry, whole blood must be rotated into its different component by being centrifugated sometimes
Layer, then can be analyzed.These layers are mainly red by blood plasma, the buffy coat containing leucocyte and blood plasma and hematocrit
Cell (PRBC) is constituted.
In addition to above-mentioned listed, packed red cells are due also to many reasons are particularly important.In immunohematology, need
PRBC carries out some type of test.PRBC usually with compared with the saline diluent suitably prepared at 0.8% and 4.0% it is dilute
The dilute form for releasing ratio uses.Draw PRBC, it is necessary to make to measure the plasma layer that probe passes through centrifugal blood.
Therefore, from the point of view of blood transfusion and the two viewpoints of immunohematology, it is important to be able to identify PRBC, and make itself and its
He separates in blood constitutent.It identifies PRBC and the known method for separating it with other blood constitutents includes：Tested (such as
The typing of blood) or test after imaging system is used to carry out operator's observation before.Test shortage accuracy after such, and if
Separation is incomplete or has drawn the layer of mistake, needs to re-start test.From the point of view of blood transfusion and the two viewpoints of immunohematology,
Other situations being able to detect in centrifugal blood, such as the error condition being not exclusively centrifugated or other error conditions, and
It is very important.
It there is a need for being able to PBRC floor and other blood constitutent areas to distinguish after different blood constitutent layers, such as centrifuge separation
It separates, and can determine other situations that may be present in centrifugation of blood samples.
Summary of the invention
The present invention relates to the method for the processing above problem, this method can determine the situation in centrifugal blood, such as will
PRBC layers are come with the identification of other centrifugal blood components.
The method that one aspect of the present invention relates to determining situation in blood sample.This method includes：Blood sample is provided；It provides
Metering probe with pump, the pump is for drawing and distributing；Probe, which will be measured, with selected distance is inserted into blood sample；It is drawn in sample
Or the pressure between measuring sample during sample distribution and pumping；Measurement pressure is compared with reference value；And there are shapes
Condition or there is no send signal when situation.In a preferred embodiment, reference value includes pressure distribution, pre-selection pressure model
It encloses or the ratio of pressure distribution preselected portions drops.
Another aspect of the present invention is provided for confirming or detecting in centrifugation of blood samples with the presence or absence of selected blood group
The method of layering.This method includes：The pressure of doubtful selected layer in probe is drawn or is measured during distributing in measurement；Pressure will be measured
It is compared with reference value；Wherein if measurement pressure is essentially identical with reference value, selected blood constitutent layer is confirmed.One
In a preferred embodiment, reference value is the pressure limit of pre-selection.
In another preferred embodiment, selected layer is packed red cells, other layers be blood plasma, and for confirm or
It detects in centrifugation of blood samples and further includes with the presence or absence of the method for selected blood constitutent layer：It is moved relative to each other the spy of metering probe
Needle top and centrifugation of blood samples will measure probe and be located in above centrifugation of blood samples；Make to measure probe and sample moves relative to each other
It is dynamic, probe will be measured and shift to blood sample surface；Test sample surface；Blood plasma is drawn from plasma layer；Drawing the same of plasma layer
When measure pressure；Reference value is compared with measurement pressure to confirm whether the layer is blood plasma；Aspiration probes top is moved into
In doubtful packed red cells layer；It draws doubtful layer and measures pressure；And reference value is compared with measurement pressure to confirm
Whether the doubtful layer being drawn is selected packed red cells layer.
An additional aspect of the present invention is provided for detecting the boundary in centrifugation of blood samples between blood plasma and packed red cells layer
The method in face.This method includes：Metering probe is located in above centrifugation of blood samples；It is moved relative to each other probe and sample,
So that probe is mobile towards sample and enters sample；Measure the first pressure of the first depth in sample；Second is deep in measurement sample
Second pressure at degree；And compare the first and second pressure, to determine whether the interface is located at the first depth and the second depth
Between.
By detailed description of preferred embodiment hereafter, the more objects, features and advantages of the present invention are for this field skill
It is obvious for art personnel.
Detailed description of the invention
Fig. 1 is shown can probe top in the conventional metered dose probe and sample container that preferred embodiment uses according to the present invention
The schematic diagram at end.
Fig. 2 shows be distributed according to the Pressure versus time pressure of blood plasma in the suction process of preferred embodiment and PRBC.
Fig. 3 shows the Pressure versus time pressure of blood plasma and PRBC in the assigning process according to preferred embodiment and is distributed.
Fig. 4 show according to preferred embodiment, when continuously drawing PRBC and blood plasma in same tips of probes blood plasma and
The Pressure versus time pressure of PRBC is distributed.
Fig. 5 show according to preferred embodiment, when continuously drawing blood plasma and PRBC in same tips of probes blood plasma and
The Pressure versus time pressure of PRBC is distributed.
Fig. 6 shows the Pressure versus time pressure of blood plasma and PRBC point in multiple suction process according to preferred embodiment
Cloth.
Specific embodiment
Although it is according to the present invention for by measurement pressure come determine situation in centrifugation of blood samples (such as confirmation or detection select
Determine blood constitutent) various applications be not restricted by, but specific available application is still led in immunohematology or the technology of blood transfusion
Within the scope of domain.The instrument that can be used in conjunction with the invention and method in extensive range will simultaneously discuss in more detail below.
The present invention determines sample using the viscosity difference indicated by the pressure difference between situations different in centrifugal blood
In whether there is particular condition.For example, in a preferred embodiment, the present invention uses selected blood constitutent and other blood groups
/ viscosity difference, so as to detect whether to have selected blood constitutent and select blood during can determine metering matters
The absorption and distribution condition of liquid component.
In a preferred embodiment, the present invention uses PRBC (being known as RBC in figure) and other blood constitutent (predominantly blood
Slurry) between viscosity difference, so as to detect whether there are PRBC and during being able to confirm that metering matters select blood constitutent
Absorption and distribution condition.Blood constitutent is mainly described as selected situation by rest part described herein, in particular, by blood
Slurry or PRBC are described as selected blood constitutent.However, thus the present invention is not restricted, as long as blood constitutent centrifugation enters certain
One layer and have different from other blood constitutent layers viscosity, then any other blood constitutent can be selected layer.For example, choosing
Determining blood constitutent can be the buffy coat containing leucocyte and blood platelet.In addition, by measurement pressure and with reference to duty pressure it
Between pressure difference determine any other situation be also covered by the scope of the invention in.
According to the concentration of PRBC, for the viscosity of PRBC usually in 8-12 centipoise (cp) or higher range, the viscosity is significant
Greater than the viscosity of blood plasma, the viscosity of blood plasma is usually in the range of less than 2 to 3cp.It is believed that PRBC has the reason of bigger viscosity
It is since it is with higher solid (i.e. red blood cell) concentration, with higher resistance to flow.In general, in PRBC layers
Concentration is about 80% red blood cell and 20% blood plasma.
Since PRBC has bigger resistance to flow (it is assumed that other situations, as absorption/apportionment ratio keeps identical), therefore
During metering matters (draw or distribute), the differences in viscosity between PRBC and blood plasma will form different pressure distributions.In other words
It says, compared with blood plasma, it is necessary to which its flowing can just be made by applying bigger pressure to PRBC.Fig. 2 shows the pressure of blood plasma and PRBC
Difference in distribution.The pressure can be monitored by the pressure sensor (such as pressure transducer) in metering system, the metering
System includes the metering probe that can be used for drawing and/or distributing.Suitable conventional metered dose including pressure sensor and metering probe
System has in (for example) United States Patent (USP) No.6,484,556,6,060,320,5,750,881,5,540,081 and 7,361,509
Described, the full text of these patents is herein incorporated by reference.Only requirement is that absorption or distribution liquid can be measured
Pressure between the pumping mechanism of measuring equipment in space.This is usually using between absorption/distribution tips of probes and pump
Pressure transducer realize, as shown in Figure 1.Fig. 1 is the schematic diagram that probe was drawn/distributed to combined type, which includes suction pipe
10, the pressure transducer 20 for pressure between measuring pump 30 and liquid (being in this case centrifugation of blood samples).The spy
Needle further includes positioned at the disposable metering top 40 of pipette tip.In the figure, in the PRBC in metering top insertion test tube 50
Close to the position of test tube bottom.Test tube contains the centrifugal blood that height is A, which is separated into blood plasma fractions 60 and height
Degree is the part PRBC 70 of B.
It executes according to the method for the present invention, obtains whole blood, it is then using centrifuge and ripe using those skilled in the art
The technology known is centrifuged.Whole blood is usually centrifuged in the collection containers of such as test tube.
After blood is separated into its component layers, selected layer can usually be separated from other components by drawing.
For example, the bottom of collection containers will be located at as the PRBC layer of most intensive component.At this point, according to the side of performed method
Metering probe can be moved to an off multiple points in heart blood by face.
In the one PRBC layers preferred embodiment for selected layer, will metering probe to be moved to collection containers bottom attached
Closely, the preferred position away from several millimeters of container bottom.If correctly executing centrifuge separation, PRBC will be located at the bottom of container.
The pump of metering probe will start and start PRBC sucking top.Top can be disposable or fixed (i.e. permanently).One
The advantage on secondary property top is not needing complicated tips of probes cleaning system, and farthest reduces in different blood samples
Between the chance that continues to use.Typical disposable top includes Microtip and VersatipTM, both tops are by Ortho-
Clinical Diagnostics, Inc. are sold, and in (for example) United States Patent (USP) No.6,797,518 and U.S. for having announced it is special
It is described in benefit application No.2003-0022380 A1, the full text of the two patents is herein incorporated by reference.If
Tips of probes be it is fixed, then can be used described in the U.S. Patent application No.2005-0196867 A1 such as announced
Those probes and probe cleaning system.
After PRBC is sucked top, first pressure distribution will be generated.It is surveyed by the pressure transducer being connected with metering probe
Pressure distribution is obtained, and can be recorded by the computer being connected with metering probe.It can make in the comparison being described below
It is distributed with complete pressure, or the Chosen Point that more preferably a part of pressure distribution or pressure can be used to be distributed.At one
One or more pressure measuring values in preferred embodiment, during can recorde metering matters at known time.In another reality
It applies in example, can determine and record the ratio drop of pressure distribution selected part.
Now it is necessary to by the first pressure generated for PRBC distribution (or pressure measuring value or ratio drop) and reference value or pressure
Power is compared.The form of reference value will depend on measured and record object.For example, if measured value is complete pressure
Distribution will be compared with pressure distribution, therefore reference value will be distributed for second pressure.If measured value
For the selected pressure measuring value in distribution map, then comparing will be compared with pressure measuring value, therefore reference value can be single
A measured value or some pressure limit for forming upper pressure limit and low pressure limit.Equally, if measured value is the one of pressure distribution
Partial ratio drop, then comparing will be compared with the ratio drop of the same section of pressure distribution.Can also with it is above two or more
A variety of combinations are compared.
As described above, it is distributed with second pressure if the measured value during metering matters is first pressure distribution
It is compared to ensure that PRBC has been drawn.Second pressure distribution can derive from multiple sources.Second pressure distribution can be
The preparatory measurement distribution map of blood sample derived from another blood plasma and/or PRBC can store in the meter with control metering probe
In the connected storage device of calculation machine.Alternatively, second pressure distribution can be plasma layer in identical centrifugation of blood samples
Observed pressure distribution (this is it is usually because most of test distribution map is all absorption blood plasma first).Computer will measure
The distribution of one pressure is compared with second pressure distribution.The combination of a variety of above-mentioned technologies can also be used to be compared to obtain more
Strong conclusion.
If second pressure distribution is the distribution map of PRBC, and the distribution map is pressed with first believed as PRBC distribution map
Power distribution is substantially the same, then this will illustrate or confirmation PRBC is actually the object being drawn, and then can be by required
Purpose uses the PRBC being drawn, such as above-mentioned application.As used herein, " substantially the same " refers to measurement pressure and ginseng
Difference≤20% of value is examined, more preferably≤10%, and most preferably≤5%.If second pressure distribution is point of blood plasma
Butut, and the distribution map differs markedly from the first pressure distribution believed as PRBC distribution map, then and this will explanation or confirmation
PRBC is actually the object being drawn, and then can use the PRBC being drawn by required purpose, such as above-mentioned
Using.As used herein, " significantly different " refers to the 25% of the difference of measurement pressure and reference value, more preferably >=30%, more
Preferably >=35%, and most preferably >=40%.Table 1 has been illustrated below, this is shown more clearly with chart mode
Concept.
Table 1- pressure distribution table
If pressure distribution is inconsistent with above-mentioned distribution map, illustrate that PRBC is not drawn, and blood is separated into it
The operation of each component do not succeed perhaps probe be located at incorrect gauge height or fluid components level be not be expected
It is horizontal.For example, it may be possible to the case where being not exclusively centrifugated or not being centrifugated occurred.In this case, first pressure point
Cloth (or other measured values) will be similar to that whole blood pressure distribution, and such pressure distribution will between blood plasma pressure be distributed and
Between the distribution of PRBC pressure.Metering probe can indicate mistake, for example, by alarm or pause metering probe or with metering probe
The operation of connected instrument, operator or instrument can take operation appropriate at this time, such as further investigated or opened again
Begin the process.Hereafter the other conditions that may cause erroneous condition are described in more detail.
Although the description in context, which is related to for pressure being distributed, is used as measured value and record value, institute above also can be used
Other measured values stated (such as than drop, discrete pressure measured value).Alternatively, as described above, it is complete with measurement
Pressure distribution on the contrary, can draw or distribute start after measure specified time at discrete pressure measured value, be then compared
To check that measurement pressure is within a predetermined range or outside preset range.The range is determined by empirical test.
According to another aspect of the present invention, plasma layer and PRBC layers are all sucked out, are used subsequently to such as typing of blood.?
In this case, usable detection method is confirmed whether that two layers are successfully sucked out.Whole blood sample is centrifuged with will be red as described above
Cell is separated from blood plasma.Sample is present in the collection containers for testing/separating, and the volume of a container/fluid is high
It spends possibly unknown.
Such as above-mentioned metering probe level is mobile to be located in its own above sample, and is moved to blood vertically downward
The surface of slurry.Although metering probe is moved relative to sample as described above in the present embodiment as described herein and other embodiments
It is dynamic, it is to be understood that only requirement is that metering probe and sample are in contact with each other.This again may be by sample container along fixation
The direction for measuring probe is mobile to realize.In a preferred embodiment, the top surface of probe sensing blood plasma.Measure top
It can be used any amount of known technology, such as in United States Patent (USP) No.5,273,717,5,143,849,5,133,392,5,
Those technologies described in 111,703 and 4,272,482 sense the top of liquid.
Once finding flow surface, the blood plasma of volume needed for metering probe will be drawn is used for subsequent applications.It is walked in the absorption
During rapid, such as by using the pressure inside above-mentioned pressure transducer measurement top, and pressure measuring value is generated, such as schemed
The distribution of first pressure shown in 2.Reference value is generated as described above, and such as second pressure is distributed.Compare pressure measuring value and reference
Value, the distribution of such as the first and second pressure.It is blood plasma or PRBC according to second pressure distribution, pressure is distributed as substantially
It is identical or dramatically different.As described above, if the comparison between the distribution of the first and second pressure is shown, and blood plasma is not sucked out,
Produce error signal.Otherwise, metering probe will then extract top out from fluid, and continue to distribute blood plasma as needed.
Draw plasma layer it is at least some after, will draw PRBC layers.If do not completed, probe is measured by removing and is used for blood plasma
The metering top of aspirate, and change new disposable top.In the case where non-disposable metering top, top can be in blood
It is washed between slurry aspirate and PRBC aspirate.As described above, metering probe is moved in sample container vertically downward, and lead to
Any remaining blood plasma is crossed, until in several millimeters away from sample container bottom.As described above, metering probe is drawn in PRBC and measurement
Portion's pump/top pressure, to obtain pressure measuring value, such as first pressure is distributed.Reference value, the such as second pressure are generated as described above
Power distribution.Compare pressure measuring value and reference value, the distribution of such as the first and second pressure.It is blood plasma according to second pressure distribution
Or PRBC's, pressure is distributed as substantially the same or dramatically different.As described above, if the first and second pressure are distributed
Between comparison show PRBC be not sucked out, then produce error signal.Otherwise, metering probe will then extract top out from fluid
End, and continue to distribute PRBC as needed.
Although the present embodiment is described as drawing plasma layer first, PRBC layers can be equally drawn first, then draws blood
Pulp layer.
According to another aspect of the present invention, the present invention can be used for positioning blood plasma/bed boundary PRBC of Centrifuge A sample.It is practical
On, metering probe can pass through Centrifuge A sample vertically downward.During passing through sample, continuously or in the preselected of entire sample depth
Point pipette samples.Sampling is more frequent, and the detection at interface is more accurate, therefore continuous pressure measured value is preferred.When metering probe
Top when reaching interface, pressure measuring value will rapidly change, and such as have reached interface by the large change instruction than drop.
The effective application of the present invention in this respect is to provide the hematocrit value percent value of Patient Sample A.Red blood cell ratio
Hold (Ht or HCT) or hematocrit (PCV) or red cell body fraction (EVF) is red blood cell ratio shared in blood volume
Example.The value is about 48% generally for male, is 38% for women.The value and hemoglobin concentration, white blood cell count(WBC) and
Platelet count is considered as the integral part of people's Complete blood count results together.The volume of packed red cells is obtained divided by the total volume of blood sample
To PCV.Because having used test tube, this can be calculated by the length of measurement layer.As an example using Fig. 1, HCT or pcv value can
By the way that PRBC layers of B are determined divided by blood sample total volume A.
Referring now to non-limiting embodiment shown in the drawings.Fig. 2 shows the pressures of blood plasma and PRBC in suction process
Power (being indicated with voltage) is distributed than the pressure of time (millisecond).The pressure distribution of blood plasma is shown with square (■) and labeled as song
The pressure of line B, PRBC, which are distributed, to be shown with diamond shape (◆) and labeled as curve A.In the present example, 25 μ L are drawn from centrifugation of blood samples
Blood plasma and 25 μ L PRBC.If Fig. 2 is clearly shown, the pressure change of the higher PRBC of viscosity is bigger.
Fig. 3 shows the pressure (voltage) of blood plasma and PRBC in assigning process and is distributed than the pressure of time (millisecond).Blood plasma
Pressure distribution shown with square (■) and labeled as curve B, and the pressure distribution of PRBC is shown and is marked with diamond shape (◆)
It is denoted as curve A.In the present example, 25 μ L blood plasma and 25 μ L PRBC are distributed from centrifugation of blood samples.If Fig. 3 is clearly shown, viscosity
The pressure change of higher PRBC is bigger.
Fig. 4 is shown using identical disposable top during identical suction process, successively draws blood plasma and PRBC
Example.As shown on the left side of the figure, the beginning of absorption is PRBC, and as shown on the right, the second part of absorption is blood plasma.Y-
Axis indicates the relative pressure in normal specimens between two kinds of different fluid types, which has been centrifuged, so that all red blood cells
In the bottom of sample container.X- axis is time (millisecond).As illustrated in FIG. 4, when reaching interface, pressure, which exists, not to be connected significantly
Continuous property.The PRBC and successively drawing for blood plasma are used especially for positioning the interface between blood plasma and PRBC layers.
Other than absorption sequence is opposite, Fig. 5 is roughly the same with Fig. 4.This shows that pressure difference can be drawn independently of fluid type
Sequentially, for identifying which kind of fluid type is in where.In fact, this method can be used for searching for blood plasma and the interface of PRBC, remove
Except this, it may also be used for determine the hematocrite value of particular patient.
Fig. 6 shows the various pressure distribution of the Pressure versus time of blood plasma and PRBC in suction process, wherein drawing 10 μ
Before L blood plasma or PRBC, have 200 μ L diluent fluids in top.First three distribution map since top is blood plasma, and
And following two distribution maps are PRBC.As shown, the pressure of blood plasma and PRBC are distributed it even if having diluent in top
Between difference still highly significant.This is highly useful discovery, because usually to it before PRBC is added to testing element
It is diluted.According to above-mentioned discovery, one aspect of the present invention provides the streamline form method of combination PRBC and diluent.More
It says to body, according to preferred embodiment, probe is inserted on top, and be moved to diluent source.The diluent of selected amount is sucked
Top.After diluent is sucked top, top is moved into centrifugation of blood samples.Probe moves to the bottom of centrifugal blood, i.e. PRBC layers
Desired location.Metering system is in fact PRBC using the layer that present invention confirmation is sucked out.PRBC sucking is included into diluent
Behind top, diluent and PRBC are distributed to groove and mixed.Then, identical top distributes diluted PRBC to test member
Part.
According to another aspect of the present invention, the present invention can be used for determining other situations that may be present in centrifugal blood.Example
Such as, it measures pressure difference or it is insufficient, can be used for determining whether the centrifugation of sample is appropriate.If it is improper not to be centrifuged or be centrifuged, pressure
Measured value is same or similar with whole blood sample, and there are the differences of very little between the pressure measuring value of sample bottom at the top of sample
It is worth or without difference.Alternatively, the pressure limit that pressure measuring value can be such as preselected with reference value is compared.
If preselected pressure limit is PRBC or blood platelet, and pressure measuring value is except preselected range, then be from
The heart improperly indicates.Alternatively, if preselected range is the pressure (pressure of such as whole blood being not exclusively centrifuged
Range), then pressure measuring value will be in preselected range.
It can be compared by measurement pressure and with the reference value such as distribution of another pressure or preselected pressure limit
Other situations to determine can comprise determining whether the pre-separation of existing blood constitutent.For example, if plasma layer from
PRBC layers of separation, and removing from sample container, then pressure measuring value will be corresponding with PRBC layer those of expected value, regardless of spy
In the sample whether pin position.If finding plasma layer, pressure measuring value deficiency correspondingly will trigger erroneous condition.Phase
Same process only just keeps authenticity when plasma layer exists and finds PRBC layers.
Another situation that can be determined by measurement pressure is the apical acquisition of the whether measured probe of fibrinogen.When
When fibrinogen sucks probe with fluid, fibrinogen will generate resistance to flow and (tend to that fluid is made to become more tacky
It is thick), this will need metering pump to provide bigger pressure could draw fluid.This bigger resistance to flow and measurement gained pressure
The ability of power is, it can be achieved that absorption to the fibrinogen (or any other particle) detected in fluid layer.In order to complete this
Class detection imports metering probe in centrifugation of blood samples layer.When drawing plasma layer, by measurement pressure or distribution map and not fibre-bearing
The reference value of the fluid of proteinogen is compared.If measurement pressure indicates higher viscosity, possible fibrinogen or its
He measures probe as blood plasma sucks by particle.In the case, erroneous condition can be indicated.
If indicating erroneous condition, as described above, metering probe can indicate mistake, such as measured by alarm or pause
Probe or the operation of instrument being connected with metering probe, operator or instrument can take operation appropriate at this time, for example, carry out into
One step is looked into or is restarted the process.
It will be apparent to those skilled in the art that can various modification can be adapted to the compound of the present invention, composition and treatment process and
Change.Thus, intend covering these modifications and changes by the present invention, as long as these modifications and changes fall into appended claims
And its range of equivalent.
The disclosure full text of all publications cited above is clearly incorporated herein by reference, like they are each
From being incorporated by reference.
Claims (11)
1. a kind of method that packed red cells layer is drawn from centrifugation of blood samples, the method includes：
The sample container for having centrifugation of blood samples is provided, the centrifugation of blood samples be separated into blood plasma fractions at the top of centrifugation of blood samples and from
The packed red cells part of painstaking effort sample bottom；
Probe will be measured, centrifugation of blood samples is inserted into selected distance, close to centrifugation of blood samples bottom；
The pressure of measurement based on centrifugation of blood samples generates first pressure distribution；
By being at least partly compared with the reference value for being related to second pressure distribution for first pressure distribution；With
If first pressure distribution is substantially the same with reference value, the hematocrit of volume needed for drawing from centrifugation of blood samples bottom is red thin
Born of the same parents arrive metering probe in part, leave the blood plasma fractions at the top of centrifugation of blood samples in sample container.
2. diluent is sucked according to the method described in claim 1, it is included in front of metering probe insertion centrifugation of blood samples
Measure probe.
3. according to the method described in claim 2, it includes from metering probe that the hematocrit of absorption mixed with diluent is red thin
Born of the same parents distribute to individual testing element part.
4. according to the method described in claim 1, wherein reference value is complete second pressure distribution.
5. according to the method described in claim 1, wherein reference value is the preselected portions of second pressure distribution.
6. according to the method described in claim 1, wherein reference value is the ratio drop of second pressure distribution preselected portions.
7. according to the method described in claim 1, wherein second pressure distribution derives from the pressure measured in advance point of another blood sample
Cloth.
If 8. according to the method described in claim 1, it include first pressure distribution be markedly different from reference value, issue mistake
Accidentally signal existing for situation.
9. according to the method described in claim 8, wherein the erroneous condition is caused by being not exclusively centrifuged.
10. according to the method described in claim 8, wherein the erroneous condition is caused by the fibrinogen in centrifugation of blood samples.
11. according to the method described in claim 1, wherein generating first pressure distribution includes carrying out multiple pressure measurement.
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| CN201810560634.0A CN108931629B (en) | 2012-02-07 | 2012-02-07 | Determining conditions in centrifuged blood using measured pressure |
| CN2012100330587A CN103245774A (en) | 2012-02-07 | 2012-02-07 | Method for determining centrifugal blood conditions through measured pressure |
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| CN103245774A (en) | 2013-08-14 |
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