CN108913544A - Pathogenic microorganism efficiently concentrating device and its enrichment method in a kind of water environment - Google Patents
Pathogenic microorganism efficiently concentrating device and its enrichment method in a kind of water environment Download PDFInfo
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- CN108913544A CN108913544A CN201810870930.0A CN201810870930A CN108913544A CN 108913544 A CN108913544 A CN 108913544A CN 201810870930 A CN201810870930 A CN 201810870930A CN 108913544 A CN108913544 A CN 108913544A
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 298
- 244000000010 microbial pathogen Species 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims description 28
- 238000001914 filtration Methods 0.000 claims abstract description 76
- 239000003480 eluent Substances 0.000 claims abstract description 66
- 238000010828 elution Methods 0.000 claims abstract description 60
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 47
- 239000008399 tap water Substances 0.000 claims description 17
- 235000020679 tap water Nutrition 0.000 claims description 17
- 239000000463 material Substances 0.000 claims description 11
- 230000008569 process Effects 0.000 claims description 8
- 239000010865 sewage Substances 0.000 claims description 8
- 229910001220 stainless steel Inorganic materials 0.000 claims description 7
- 239000010935 stainless steel Substances 0.000 claims description 7
- 238000002604 ultrasonography Methods 0.000 claims description 6
- 238000011001 backwashing Methods 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 5
- 230000009514 concussion Effects 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims description 4
- 239000013535 sea water Substances 0.000 claims description 4
- 239000002352 surface water Substances 0.000 claims description 4
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 3
- 239000000872 buffer Substances 0.000 claims description 3
- 239000000919 ceramic Substances 0.000 claims description 3
- 239000000839 emulsion Substances 0.000 claims description 3
- 229920006351 engineering plastic Polymers 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 239000008363 phosphate buffer Substances 0.000 claims description 3
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 3
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 3
- 229920001296 polysiloxane Polymers 0.000 claims description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical class OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 3
- 239000004094 surface-active agent Substances 0.000 claims description 2
- 238000001514 detection method Methods 0.000 abstract description 17
- 238000012544 monitoring process Methods 0.000 abstract description 2
- 230000000813 microbial effect Effects 0.000 abstract 1
- 241000224467 Giardia intestinalis Species 0.000 description 18
- 229940085435 giardia lamblia Drugs 0.000 description 18
- 241000607142 Salmonella Species 0.000 description 17
- 241000223936 Cryptosporidium parvum Species 0.000 description 14
- 210000003250 oocyst Anatomy 0.000 description 14
- 241000607768 Shigella Species 0.000 description 13
- 244000005700 microbiome Species 0.000 description 12
- 238000011084 recovery Methods 0.000 description 11
- 238000004064 recycling Methods 0.000 description 10
- 230000001954 sterilising effect Effects 0.000 description 9
- 238000004659 sterilization and disinfection Methods 0.000 description 9
- 201000010099 disease Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 239000002775 capsule Substances 0.000 description 5
- 238000004506 ultrasonic cleaning Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 235000020188 drinking water Nutrition 0.000 description 4
- 239000003651 drinking water Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 210000004215 spore Anatomy 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 102000002322 Egg Proteins Human genes 0.000 description 3
- 108010000912 Egg Proteins Proteins 0.000 description 3
- 241000589902 Leptospira Species 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 210000004681 ovum Anatomy 0.000 description 3
- 241000700605 Viruses Species 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000003463 adsorbent Substances 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 239000004800 polyvinyl chloride Substances 0.000 description 2
- 239000008400 supply water Substances 0.000 description 2
- 241000223935 Cryptosporidium Species 0.000 description 1
- 108010034145 Helminth Proteins Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000002337 anti-port Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000008394 flocculating agent Substances 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 244000000013 helminth Species 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000001471 micro-filtration Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 229910052573 porcelain Inorganic materials 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
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- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The present invention relates to pathogenic microorganism efficiently concentrating devices in water environment, belong to water environment technical field of microbial detection.The enriching apparatus includes filtration fraction and control section, and filter core is placed in filter core barrel bore in filtration fraction and there are spaces between filter core and filter core barrel bore wall；Filter core sleeve top is provided with water sample water inlet；Filter core bottom end is provided with water sample water outlet；The inner wall that water sample water outlet passes through filter core sleeve extends to outside；Control section includes threeway, water sample filtering control section and elution control section；Water sample filters control section and has been sequentially communicated the first check valve and water sample filtering peristaltic pump according to water sample elution order；Elution control section has been sequentially communicated elution flow container, elution peristaltic pump and second one-way valve according to eluent inflow direction；Water sample water outlet, the first check valve are connected to second one-way valve by threeway.It realizes the efficiently concentrating to low concentration pathogenic microorganism in water environment, provides technical support for the pathophorous detection of Jie's water, monitoring.
Description
Technical field
The invention belongs to the pathogenic microorganism examination technical fields in water environment, and in particular to the micro- life of cause of disease in a kind of water environment
Object efficiently concentrating device and its enrichment method.
Background technique
All kinds of pathogenic microorganisms (including bacterium, protozoon, virus etc.) are that Jie's water is caused to spread the disease present in water environment
The main reason for outburst, not only endangers population health, can also bring serious economic loss, or even generate serious bad society
It will affect.The propagation of pathogenic microorganism Jie's water can frequently result in group and break out event, and global distribution is presented, wherein causing a disease
Bacterium, helminth, virus etc. are main inducings.According to statistics, in world wide every year because caused by cause pathogeny imcrobe infection 5 years old with
Lower children's diarrhae death toll is up to more than 130 ten thousand.
Jie's water propagate pathogenic microorganism generally have the characteristics that it is pathogenic strong, low amounts intake can lead to population infection,
Its content in water environment is generally seldom simultaneously, and current common detection methods is caused to cannot achieve the accurate of pathogenic microorganism
Detection, as a result, it is often necessary to by way of enrichment, by after being enriched with to the pathogenic microorganism in water sample with bulk mass
It is able to satisfy the demand of detection.Currently, pathogenic microorganism enrichment common method has filtration method, flocculence, absorption method etc. in water environment,
In practical applications can for different experiment purposes, water body environment and select different enrichment methods.
Filtration method is pathogenic microorganism enrichment method more common at present, generally based on microfiltration membranes, ultrafiltration membrane etc., but by
It is limited in the ability that filter membrane is recycled, thus its cost is relatively high.Flocculation rule needs to come using a large amount of flocculant real
The use of the purpose being now enriched with, a large amount of flocculants can cause certain potential hazard to environment.And absorption method is due to adsorbent material
Regeneration it is comparatively laborious, influence being used repeatedly for adsorbent material.
Summary of the invention
In view of this, the purpose of the present invention is to provide pathogenic microorganism efficiently concentrating device and its richnesses in a kind of water environment
Set method can be widely used in the enrichment of pathogenic microorganism in multiple water environments using described device.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical scheme：
The present invention provides pathogenic microorganism efficiently concentrating devices in a kind of water environment, including filtration fraction and control unit
Point：
The filtration fraction includes filter core and filter core sleeve；The filter core is placed in the filter core barrel bore and the filter core
There are spaces between the internal chamber wall of the filter core sleeve；The top of the filter core sleeve is provided with water sample water inlet；The filter
The bottom end of core is provided with water sample water outlet；The inner wall that the water sample water outlet passes through filter core sleeve extends to outside；
The control section includes threeway, water sample filtering control section and elution control section；The water sample filtering control
Part has been sequentially communicated the first check valve and water sample filtering peristaltic pump according to water sample elution order；First check valve is opened
Opening direction is opened along water sample filtering peristaltic pump direction；
The elution control section according to eluent inflow direction be sequentially communicated elution flow container 9, elution peristaltic pump and
Second one-way valve；The opening direction of the second one-way valve is opened along water sample water outlet direction；
The second one-way valve of the water sample water outlet, the first check valve of water sample filtering control section and elution control section
It is connected to by threeway.
Preferably, the material of the filter core sleeve includes stainless steel, glass, PVC or engineering plastics material.
Preferably, the aperture of the filter core includes 0.45~1 μm.
Preferably, the filter core includes stainless steel filter core, stud filter core or ceramic element.
Preferably, all connections are connected to using conduit in the control section.
Preferably, the material of the conduit includes emulsion tube, silicone tube, PVC hose etc..
Preferably, the enriching apparatus further includes cabinet；The filtration fraction and control section are located at the cabinets cavity
In.
Preferably, the filtration fraction is removably attached in the inner cavity of cabinet.
Preferably, the method being removably secured includes screw, hasp or pipe collar.
Preferably, the water sample water inlet 4 extends to 10 outside of cabinet by conduit.
The present invention provides a kind of methods based on pathogenic microorganism in described device enriched water environment, including following step
Suddenly：
1) flow velocity of water sample to be detected is controlled with water sample filtering with peristaltic pump, the flow velocity for setting water sample to be detected passes through
Water sample water inlet enters the space between filter core sleeve and filter core, and the filtering of water sample is then realized through filtration core, filtered
Water sample is discharged by water sample water outlet；
2) after water sample to be detected filters, made to be contained in washing in elution flow container with elution peristaltic pump coutroi velocity
De- liquid enters the intermediate space inside filter core from water sample water outlet with the flow velocity that sets, to make eluent in a manner of backwashing
The space between filter core and filter core sleeve is reached, the process of backwash is completed；
3) after process subject to backwash, water sample water inlet and water sample water outlet is closed, will be contained in the step 2)
The filtration fraction of eluent using ultrasound or concussion carry out elutriation processing, the eluent after elutriation is handled in filtration fraction from
The discharge of water sample water inlet, collects eluent, pathogenic microorganism after being enriched with.
Preferably, in the step 1) water body to be detected include tap water, water supply water, sewage, recycled water, it is secondary go out
Water, underground water, surface water, precipitation or seawater.
Preferably, it is 4.5~9.8 that the eluent, which is pH value, the non-ionic surface that mass concentration is 0.01~0.1%
The phosphate buffer or Tris salt buffer of activating agent Tween-20.
The present invention provides pathogenic microorganism efficiently concentrating devices in a kind of water environment, including filtration fraction and control unit
Point, wherein using peristaltic pump to control water sample to be detected enters sleeve and filter by the water sample water inlet on sleeve with certain flow velocity
Space between core, and enter by filter element filtering face the intermediate cylinder of filter core, it is discharged from water sample water outlet.During elutriation,
Peristaltic pump controls eluent back through filter core by antiport, to realize washing for the microorganism for being enriched in cartridge surface
It is de-.Enriching apparatus provided by the invention has by selecting the filter core of different pore size to realize the enrichment of multiple-microorganism using wide
General feature.Enriching apparatus provided by the invention makes full use of the advantages of filter element filtering technology, then complement filter control section and
Control section is eluted, enrichment is realized and is eluted in same device and complete, while the set-up mode of filtration fraction of the present invention, meet
The filtering of water sample with bulk mass passes to realize the efficiently concentrating of low concentration pathogenic microorganism in water environment for water environment intermediary water
Broadcast the detection of disease, monitoring provides technical support.
Enriching apparatus provided by the invention has the characteristics that precision is high, flow is big, structure is uniform simultaneously, further, pottery
Porcelain filter core, stainless steel filter core, stud filter core etc. also have the advantages such as corrosion resistance, wearability be strong, and it can be used repeatedly, significantly reduces
Use cost.
The present invention provides a kind of method based on pathogenic microorganism in described device enriched water environment, the method operations
Simplicity, effect is reliable, it is big to be capable of handling volume of water sample, and can recycle reuse, production cost is effectively reduced.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of enriching apparatus of the present invention, in figure：1. filter core；2. filter core sleeve；3. water sample mistake
Peristaltic pump is used in filter；4. water sample water inlet；5. water sample water outlet；6. threeway；7. peristaltic pump is used in elution；8. second one-way valve；9. washing
De- flow container；10. cabinet；11. the first check valve.
Specific embodiment
The present invention provides pathogenic microorganism efficiently concentrating devices in a kind of water environment, as shown in Figure 1, including filtration fraction
The control section and：The filtration fraction includes filter core and filter core sleeve；The filter core is placed in the filter core barrel bore and described
There are spaces between filter core and the internal chamber wall of the filter core sleeve；The top of the filter core sleeve is provided with water sample water inlet；Institute
The bottom end for stating filter core is provided with water sample water outlet；The inner wall that the water sample water outlet passes through filter core sleeve extends to outside；It is described
Control section includes threeway, water sample filtering control section and elution control section；Water sample filtering control section is according to water sample
Elution order has been sequentially communicated the first check valve and water sample filtering peristaltic pump；The opening direction of first check valve is along water
Spline filter is opened with peristaltic pump direction；The elution control section according to eluent inflow direction be sequentially communicated elution flow container,
Elution peristaltic pump and second one-way valve；The opening direction of the second one-way valve is opened along water sample water outlet direction；
The second one-way valve of the water sample water outlet, the first check valve of water sample filtering control section and elution control section passes through threeway
Connection.
In the present invention, the material of the filter core sleeve preferably includes stainless steel, glass, PVC or engineering plastics material.
In the present invention, the aperture of the filter core preferably includes 0.45~1 μm.It is selected not according to different types of microorganism
Filter core with aperture is enriched with.The filter core preferably includes stainless steel filter core, stud filter core or ceramic element.
In the present invention, all connections are preferably connected to using conduit in the control section.The material of the conduit is preferred
Including emulsion tube, silicone tube, PVC hose etc..The present invention is not particularly limited the method for the connection, using known to field
Connectivity scheme.
In a preferred embodiment of the invention, the enriching apparatus further includes cabinet；The filtration fraction and control unit quartile
In the cabinets cavity.The filtration fraction is removably attached in the inner cavity of cabinet.The side being removably secured
Method includes screw, hasp or pipe collar etc..The water sample water inlet extends on the outside of the cabinet.
The present invention provides a kind of methods based on pathogenic microorganism in described device enriched water environment, including following step
Suddenly：
1) flow velocity that water sample to be detected is controlled with water sample filtering with peristaltic pump, makes water sample to be detected pass through water with certain flow rate
Sample water inlet enters the cavity between filter core sleeve and filter core, then realizes and filters through filtration core, and filtered water sample is from water sample
Water outlet discharge；
2) after water sample to be detected filters, made to be contained in washing in elution flow container with elution peristaltic pump coutroi velocity
The certain flow velocity of de- liquid enters the intermediate space inside filter core from water sample water outlet, so that de- liquid be made to reach in a manner of backwashing
The process of backwash is completed in space between filter core and filter core sleeve；
3) after process subject to backwash, water sample water inlet and water sample water outlet is closed, will be contained in the step 2)
The filtration fraction of eluent using ultrasound or concussion carry out elutriation processing, the eluent after elutriation is handled in filtration fraction from
The discharge of water sample water inlet, collects eluent, obtains being collected into centrifuge tube containing the eluent of pathogenic microorganism after enrichment, use
In the detection of next step.
The present invention controls the flow velocity of water sample to be detected with water sample filtering with peristaltic pump, and water sample to be detected is made to intake by water sample
Mouth enters in filter core sleeve, through filter element filtering, is discharged by filtered water sample from water sample water outlet.
In the present invention, the water body to be detected preferably include tap water, water supply water, sewage, recycled water, it is secondary go out
Water, underground water, surface water, precipitation or seawater.The flow velocity is preferably not higher than 5L/min, more preferably 2~4L/min, optimal
It is selected as 3L/min.
In the present invention, when the process filter element filtering, pathogenic microorganism is retained in filter element filtering in water sample to be detected
On face.The selection in the aperture of the filter core is carried out according to the size of pathogenic microorganism to be enriched with, preferred generally for pathogen
The filter core in 0.45 μm of aperture, for the filter core in preferably 1.0 μm apertures of protozoon.
In the present invention, the volume of water sample to be measured is handled according to the water quality of pending water：For tap water, water supply
It is preferably 50~100L that water, underground water, precipitation etc., which handle volume,；For sewage, recycled water, secondary water-supply, surface water, seawater etc.
Handling volume is preferably 10~50L.
After water sample filtering to be detected, present invention elution peristaltic pump coutroi velocity will be contained in elution flow container
Eluent enters the space among filter core from water sample water outlet, and the eluent reaches filter core and filter core set in a manner of backwashing
In space between cylinder, the filtration fraction containing eluent is obtained.
In the present invention, the flow velocity is preferably not higher than 5L/min, more preferably 2~4L/min, most preferably 3L/
min.The eluent is preferably that pH value is 4.5~9.8, the nonionic surface active agent that mass concentration is 0.01~0.1%
Tween-20 phosphate buffer or Tris salt buffer.The time of the elution is preferably 5~30min, more preferably
10min.Eluent will be enriched with when in the space that the eluent reaches between filter core and filter core sleeve in a manner of backwashing
After the pathogenic microorganism in filter element filtering face elutes, the eluent containing pathogenic microorganism after enrichment is obtained.
After process subject to backwash, water sample water inlet and water sample water outlet are closed, the present invention described will contain elution
The filtration fraction of liquid carries out elutriation processing using ultrasound or concussion, and the eluent after elutriation is handled in filtration fraction is from water sample
Water inlet discharge, collects eluent, obtains being collected into centrifuge tube, being used for down containing the eluent of pathogenic microorganism after enrichment
The detection of one step.
In the present invention, the parameter of the ultrasonic treatment is as follows：Ultrasonic power is in 30~100KHz, preferably 40KHz, time
For 5~45min, temperature is 10~45 DEG C.The present invention is not particularly limited the excusing from death processing with instrument, using this field institute
In well known ultrasonic cleaning equipment.
In the present invention, the rate of recovery statistical method of pathogenic microorganism is as follows in the water environment：The eluent that will be obtained
It is concentrated, is observed under the microscope or pathogenic microorganism is counted using selective medium, count the micro- life of cause of disease
The quantity of object；The quantity of obtained pathogenic microorganism is substituted into formula (1) to the recycling that pathogenic microorganism in water environment is calculated
Rate.
The cause of disease added in the rate of recovery (%)=pathogenic microorganism number/water sample to be measured of pathogenic microorganism in water environment
The quantity formula (1) of microorganism
Pathogenic microorganism efficiently concentrating device in a kind of water environment provided by the invention is carried out below with reference to embodiment detailed
Thin explanation, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
The enrichment of Cryptosporidium parvum oocysts suspended and giardia lamblia stiles sporangiocyst in 100L tap water
It takes 100L city tap-water, after 121 DEG C of high pressure sterilizations, Cryptosporidium parvum oocysts suspended and giardia lamblia stiles sporangiocyst, people is added
Work pollutes tap water, its concentration is made to respectively reach 1/L.Select 1.0 μm of aperture filter core, starting water sample filtering peristaltic pump 3, control
Water sample flow velocity 2L/min processed makes water sample enter filter core sleeve 2 from water sample water inlet 4, is filtered by filter core 1, to water sample mistake
Stop filtering peristaltic pump 3 after having filtered.
Later, eluent is packed into elution flow container 9, starts elution peristaltic pump 7, eluent is made to pass through filter core intermediate gaps
And enriched microorganism is eluted by back flush mode, gap between filter core 1 and filter core sleeve 2 is fully entered to eluent
Afterwards, filter core sleeve 2 and filter core 1 are removed, inlet and outlet is closed with clip, places it in ultrasonic cleaning machine or oscillator
Elution, recycling eluent carry out Cryptosporidium parvum oocysts suspended and the detection of giardia lamblia stiles sporangiocyst, Cryptosporidium parvum oocysts suspended and giardia lamblia stiles
The rate of recovery of sporangiocyst reaches 25%~100%.
Embodiment 2
The enrichment of salmonella and Shigella in 100L tap water
It takes 100L city tap-water, after 121 DEG C of high pressure sterilizations, salmonella and Shigella bacterium solution, artificial contamination is added
Tap water makes its concentration respectively reach 10CFU/L.Select 0.45 μm of aperture filter core, starting water sample filtering peristaltic pump 3, control
Water sample flow velocity 2L/min makes water sample enter filter core sleeve 2 from water sample water inlet 4, is filtered by filter core 1, filters to water sample
Stop filtering peristaltic pump 3 after complete.
Later, a certain amount of eluent is packed into elution flow container 9, starts elution peristaltic pump 7, eluent is made to pass through filter core 1
Intermediate gaps and the elution that enriched microorganism is carried out by back flush mode, fully enter filter core 1 and filter core sleeve 2 to eluent
Between behind gap, remove filter core sleeve 2 and filter core 1, close water sample water inlet 4 and water sample water outlet 5 with clip, place it in super
It is eluted in sound washer or oscillator, recycling eluent carries out salmonella and Shigella detection, salmonella and shiga
The bacterium rate of recovery respectively reaches 60% or more.
Embodiment 3
The enrichment of Leptospira in 100L tap water
It takes 100L city tap-water, after 121 DEG C of high pressure sterilizations, Leptospira polypide is added, artificial contamination's water sample makes it
Concentration reaches 1/L.1.0 μm of aperture filter core is selected, starting water sample filtering peristaltic pump 3 controls water sample flow velocity 2L/min, makes water
Sample enters filter core sleeve 2 from water sample water inlet 4, is filtered by filter core 1, stops filtering peristaltic pump after water sample has filtered
3。
Later, a certain amount of eluent is packed into elution flow container 9, starts elution peristaltic pump 7, pass through eluent in filter core
Between gap and the elution of enriched microorganism is carried out by back flush mode, to eluent fully enter filter core 1 and filter core sleeve 2 it
Between behind gap, remove filter core sleeve 2 and filter core 1, close water sample water inlet 4 and water sample water outlet 5 with clip, place it in ultrasound
It is eluted in washer or oscillator, recycling eluent carries out Leptospira detection, and the rate of recovery reaches 35% or more.
Embodiment 4
The enrichment of Cryptosporidium parvum oocysts suspended and giardia lamblia stiles sporangiocyst in 50L drinking water resource
It takes 50L drinking water resource (underground water), after 121 DEG C of high pressure sterilizations, Cryptosporidium parvum oocysts suspended and giardia lamblia stiles is added
Sporangiocyst, artificial contamination's tap water make its concentration respectively reach 1/L.1.0 μm of aperture filter core is selected, starting water sample is filtered with compacted
Dynamic pump 3, controls water sample flow velocity 2L/min, so that water sample is entered filter core sleeve 2 from water sample water inlet 4, is filtered by filter core 1,
Stop filtering peristaltic pump 3 after water sample has filtered.
Later, a certain amount of eluent is packed into elution flow container 9, starts elution peristaltic pump 7, eluent is made to pass through filter core 1
Intermediate gaps and the elution that enriched microorganism is carried out by back flush mode, fully enter filter core 1 and sleeve filter core 2 to eluent
Between behind gap, remove filter core sleeve 2 and filter core 1, close water sample water inlet 4 and water sample water outlet 5 with clip, place it in super
It is eluted in sound washer or oscillator, recycling eluent carries out Cryptosporidium parvum oocysts suspended and the detection of giardia lamblia stiles sporangiocyst, hidden spore
The rate of recovery of worm's ovum capsule and giardia lamblia stiles sporangiocyst reaches 25%~100%.
Embodiment 5
The enrichment of salmonella and Shigella in 50L drinking water resource
It takes 50L drinking water resource (underground water), after 121 DEG C of high pressure sterilizations, salmonella and Shigella bacterium solution is added, makes
Its concentration respectively reaches 10CFU/L.0.45 μm of aperture filter core is selected, starting water sample filtering peristaltic pump 3 controls water sample flow velocity
2L/min makes water sample enter filter core sleeve 2 from water sample water inlet 4, is filtered by filter core 1, is stopped after water sample has filtered
Filtering peristaltic pump 3.
Later, a certain amount of eluent is packed into elution flow container 9, starts elution peristaltic pump 7, pass through eluent in filter core
Between gap and the elution of enriched microorganism is carried out by back flush mode, fully enter filter core 1 to eluent and filter core sleeve 2 filtered
Between core behind gap, filter core sleeve 2 and filter core 1 are removed, water sample water inlet 4 and water sample water outlet 5 is closed with clip, places it in
It is eluted in ultrasonic cleaning machine or oscillator, recycling eluent carries out salmonella and Shigella detection, and salmonella and will are congratulated
The Salmonella rate of recovery reaches 60% or more.
Embodiment 6
The enrichment of Cryptosporidium parvum oocysts suspended and giardia lamblia stiles sporangiocyst in 20L sewage
It takes 20L city domestic sewage, after 121 DEG C of high pressure sterilizations, Cryptosporidium parvum oocysts suspended and giardia lamblia stiles sporangiocyst, people is added
Work pollutes tap water, its concentration is made to respectively reach 1/L.Select 1.0 μm of aperture filter core, starting water sample filtering peristaltic pump 3, control
Water sample flow velocity 2L/min processed makes water sample enter filter core sleeve 2 from water sample water inlet 4, is filtered by filter core 1, to water sample mistake
Stop filtering peristaltic pump 3 after having filtered.
Later, a certain amount of eluent is packed into elution flow container 9, starts elution peristaltic pump 7, pass through eluent in filter core
Between gap and the elution of enriched microorganism is carried out by back flush mode, to eluent fully enter filter core 1 and filter core sleeve 2 it
Between behind gap, remove filter core sleeve 2 and filter core 1, close water sample water inlet 4 and water sample water outlet 5 with clip, place it in ultrasound
It is eluted in washer or oscillator, recycling eluent carries out Cryptosporidium parvum oocysts suspended and the detection of giardia lamblia stiles sporangiocyst, Cryptosporidium
The rate of recovery of egg capsule and giardia lamblia stiles sporangiocyst reaches 15%~100%.
Embodiment 7
The enrichment of salmonella and Shigella in 20L sewage
It takes 20L city domestic sewage, after 121 DEG C of high pressure sterilizations, salmonella and Shigella bacterium solution is added, makes its concentration
Respectively reach 10CFU/L.0.45 μm of aperture filter core is selected, starting water sample filtering peristaltic pump 3 controls water sample flow velocity 2L/min,
So that water sample is entered filter core sleeve 2 from water sample water inlet 4, be filtered by filter core 1, stops filtering with compacted after water sample has filtered
Dynamic pump 3.
Later, a certain amount of eluent is packed into elution flow container 9, starts elution peristaltic pump 7, eluent is made to pass through filter core 1
Intermediate gaps and the elution that enriched microorganism is carried out by back flush mode, fully enter filter core 1 and filter core sleeve 2 to eluent
Between behind gap, remove filter core sleeve 2 and filter core 1, close water sample water inlet 4 and water sample water outlet 5 with clip, place it in super
It is eluted in sound washer or oscillator, recycling eluent carries out salmonella and Shigella detection, salmonella and shiga
The bacterium rate of recovery reaches, and 50% or more.
Embodiment 8
The enrichment of Cryptosporidium parvum oocysts suspended and giardia lamblia stiles sporangiocyst in 50L recycled water
Take 50L recycled water plant effluent, after 121 DEG C of high pressure sterilizations, Cryptosporidium parvum oocysts suspended and giardia lamblia stiles sporangiocyst are artificial dirty
Tap water is contaminated, its concentration is made to respectively reach 1/L.1.0 μm of aperture filter core is selected, starting water sample filtering peristaltic pump 3 controls water
Sample flow velocity 2L/min makes water sample enter filter core sleeve 2 from water sample water inlet 4, is filtered by filter core 1, has been filtered to water sample
Stop filtering peristaltic pump 3 afterwards.
Later, a certain amount of eluent is packed into elution flow container 9, and filter core sleeve 2 and filter core 1 is removed into reversed fixation, opened
Dynamic elution peristaltic pump 7, making eluent by 1 intermediate gaps of filter core and by back flush mode progress, enriched microorganism is washed
It is de-, it is fully entered between filter core 1 and filter core sleeve 2 behind gap after eluent, removes filter core sleeve 2 and filter core 1, closed with clip
Water sample water inlet 4 and water sample water outlet 5, place it in ultrasonic cleaning machine or oscillator and elute, and recycling eluent carries out hidden spore
Sub- worm's ovum capsule and the detection of giardia lamblia stiles sporangiocyst, the two rate of recovery reach 15% or more.
Embodiment 9
The enrichment of salmonella and Shigella in 50L recycled water
It takes 50L recycled water plant effluent, after 121 DEG C of high pressure sterilizations, salmonella and Shigella bacterium solution is added, makes its concentration
Respectively reach 10CFU/L.0.45 μm of aperture filter core is selected, starting water sample filtering peristaltic pump 3 controls water sample flow velocity 2L/min,
So that water sample is entered filter core sleeve 2 from water sample water inlet 4, be filtered by filter core 1, stops filtering with compacted after water sample has filtered
Dynamic pump 3.
Later, a certain amount of eluent is packed into elution flow container 9, and filter core sleeve 2 and filter core 1 is removed into reversed fixation, opened
Dynamic elution peristaltic pump 7, making eluent by 1 intermediate gaps of filter core and by back flush mode progress, enriched microorganism is washed
It is de-, it is fully entered between filter core 1 and filter core sleeve 2 behind gap after eluent, removes filter core sleeve 2 and filter core 1, closed with clip
Water sample water inlet 4 and water sample water outlet 5, place it in ultrasonic cleaning machine or oscillator and elute, and recycling eluent carries out sramana
Salmonella and Shigella detection, salmonella and the Shigella rate of recovery reach, and 35% or more.
Embodiment 10
Enrichment of the reuse filter core to Cryptosporidium parvum oocysts suspended and giardia lamblia stiles sporangiocyst in 100L tap water
Used 1.0 μm of filter cores are taken, after being impregnated with dilute acid soln, distilled water cleaning, the hidden spore being subsequently used in tap water
The enrichment of sub- worm's ovum capsule and giardia lamblia stiles sporangiocyst, and clean repeatedly, using multiple.Cryptosporidium parvum oocysts suspended and giardia lamblia stiles spore
The enriching and recovering rate of capsule reaches 10% -100%.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. pathogenic microorganism efficiently concentrating device in a kind of water environment, which is characterized in that including filtration fraction and control section：
The filtration fraction includes filter core and filter core sleeve；The filter core is placed in the filter core barrel bore and the filter core and institute
State between the internal chamber wall of filter core sleeve that there are spaces；The top of the filter core sleeve is provided with water sample water inlet；The filter core
Bottom end is provided with water sample water outlet；The inner wall that the water sample water outlet passes through filter core sleeve extends to outside；
The control section includes threeway, water sample filtering control section and elution control section；The water sample filters control section
The first check valve and water sample filtering peristaltic pump have been sequentially communicated according to water sample elution order；The opening square of first check valve
To be along water sample filtering peristaltic pump direction open；
The elution control section has been sequentially communicated elution flow container, elution peristaltic pump and the second list according to eluent inflow direction
To valve；The opening direction of the second one-way valve is opened along water sample water outlet direction；
The second one-way valve of the water sample water outlet, the first check valve of water sample filtering control section and elution control section passes through
Threeway connection.
2. enriching apparatus according to claim 1, which is characterized in that the material of the filter core sleeve includes stainless steel, glass
Glass, PVC or engineering plastics material.
3. enriching apparatus according to claim 1, which is characterized in that the aperture of the filter core includes 0.45~1 μm；It is described
Filter core includes stainless steel filter core, stud filter core or ceramic element.
4. enriching apparatus according to claim 1, which is characterized in that the mode of all connections uses in the control section
Conduit connection；The material of the conduit includes emulsion tube, silicone tube or PVC hose.
5. enriching apparatus described according to claim 1~any one of 4, which is characterized in that the enriching apparatus further includes
Cabinet；The filtration fraction and control section are located in the cabinets cavity.
6. enriching apparatus according to claim 5, which is characterized in that the filtration fraction is removably attached to cabinet
In inner cavity.
7. enriching apparatus according to claim 6, which is characterized in that the water sample water inlet is extended to described by conduit
On the outside of cabinet.
8. a kind of method based on pathogenic microorganism in claim 1~7 any one described device enriched water environment, feature
It is, includes the following steps：
1) flow velocity of water sample to be detected is controlled with water sample filtering with peristaltic pump, the flow velocity for setting water sample to be detected passes through water sample
Water inlet enters the space between filter core sleeve and filter core, the filtering of water sample is then realized through filtration core, by filtered water sample
It is discharged by water sample water outlet；
2) after water sample to be detected filters, the eluent being contained in elution flow container is made with elution peristaltic pump coutroi velocity
Enter the intermediate space inside filter core from water sample water outlet with the flow velocity of setting, eluent is made to reach filter core in a manner of backwashing
The process of backwash is completed in space between filter core sleeve；
3) after process subject to backwash, water sample water inlet and water sample water outlet are closed, elution will be contained in the step 2)
The filtration fraction of liquid carries out elutriation processing using ultrasound or concussion, and the eluent after elutriation is handled in filtration fraction is from water sample
Water inlet discharge, collects eluent, pathogenic microorganism after being enriched with.
9. according to the method described in claim 8, it is characterized in that, in the step 1) water body to be detected include tap water, from
Come source water, sewage, recycled water, secondary water outlet, underground water, surface water, precipitation or seawater.
10. method according to claim 8 or claim 9, which is characterized in that the eluent is that pH value is 4.5~9.8, quality
The phosphate buffer or Tris salt buffer for the nonionic surface active agent Tween-20 that concentration is 0.01~0.1%.
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| CN201810870930.0A CN108913544A (en) | 2018-08-02 | 2018-08-02 | Pathogenic microorganism efficiently concentrating device and its enrichment method in a kind of water environment |
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| CN112129608A (en) * | 2020-10-10 | 2020-12-25 | 奎泰斯特(上海)科技有限公司 | Water filtering and elutriating method |
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Application publication date: 20181130 |