CN108912207A - High-throughput Solid phase peptide synthssis device - Google Patents
High-throughput Solid phase peptide synthssis device Download PDFInfo
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- CN108912207A CN108912207A CN201810631764.9A CN201810631764A CN108912207A CN 108912207 A CN108912207 A CN 108912207A CN 201810631764 A CN201810631764 A CN 201810631764A CN 108912207 A CN108912207 A CN 108912207A
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- Prior art keywords
- pipe
- waste collection
- peptide
- peptide systhesis
- opening
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 53
- 239000007790 solid phase Substances 0.000 title claims abstract description 17
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 58
- 239000002699 waste material Substances 0.000 claims abstract description 37
- 239000007788 liquid Substances 0.000 claims abstract description 25
- 229910001873 dinitrogen Inorganic materials 0.000 claims abstract description 8
- 239000011229 interlayer Substances 0.000 claims abstract description 7
- 239000011521 glass Substances 0.000 claims abstract description 4
- 230000000452 restraining effect Effects 0.000 claims abstract description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 25
- 238000007789 sealing Methods 0.000 claims description 7
- 238000000926 separation method Methods 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 239000007789 gas Substances 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 abstract description 4
- 238000003786 synthesis reaction Methods 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 230000006641 stabilisation Effects 0.000 abstract description 2
- 238000011105 stabilization Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 4
- 238000010647 peptide synthesis reaction Methods 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 230000002194 synthesizing effect Effects 0.000 description 2
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention belongs to Peptide systhesis equipment technical field, in particular to a kind of high-throughput Solid phase peptide synthssis device, including constant temperature pipe support, Peptide systhesis pipe, waste collection pipe, nitrogen gas tank and waste collection bottle；The constant temperature pipe support includes the frame body with hollow interlayer, and multiple cylindrical channels are arranged on the frame body on vertical direction；Sand core plate, the liquid valve door that bottom is arranged liquid outlet and is mounted on liquid outlet are set inside the Peptide systhesis pipe；Restraining position ring block is fixed in Peptide systhesis pipe outer wall upper end；The waste collection pipe is the glass tube being arranged diagonally downward, is connected with nitrogen gas tank, vacuum pump and waste collection bottle；By the way that constant temperature pipe support is arranged, so that the Peptide systhesis pipe being fixed on inside pipe support is in temperature constant state, is conducive to the stabilization of Peptide systhesis, efficiently carries out, by the way that multiple Peptide systhesis pipes are arranged, multiple groups synthesis can be carried out simultaneously, improve production efficiency.
Description
Technical field
The invention belongs to Peptide systhesis equipment technical field, in particular to a kind of high-throughput Solid phase peptide synthssis device.
Background technique
Peptide systhesis is that a synthesis in solid state sequence is generally synthesized from N-terminal (aminoterminal) to C-terminal (c-terminus).It is past more
Peptide synthesis is the referred to as liquid phase synthesizing method carried out in the solution.Solid phase peptide synthssis is developed successfully from Merrifield in 1963
Since method, by continuous improve and perfect, one had become in peptide and protein synthesis to solid phase method today is common
Technology shows the incomparable advantage of classical liquid phase synthesizing method, to greatly alleviate the difficulty of every step product purification.
The advantages of synthesis in solid state, which is mainly manifested in initial reactants and products, to be connected on solid phase carrier, therefore can
To carry out all reactions in a reaction vessel, it is convenient for automatic operation, excessive reactant, which is added, can obtain high yield
The product of rate, while product is easily separated.Chemically synthesized polypeptide now can be in the automation Peptide synthesizer of process control
Upper progress.Commercialized Peptide synthesizer is expensive, and cannot be customized according to Research Requirements, and not side is used in scientific research
Just.
Summary of the invention
The present invention solves the above-mentioned technical problems in the prior art, provides a kind of high-throughput Solid phase peptide synthssis dress
It sets.
To solve the above problems, technical scheme is as follows：
High-throughput Solid phase peptide synthssis device, including constant temperature pipe support, Peptide systhesis pipe, waste collection pipe, nitrogen gas tank and useless
Liquid receiving flask；
The constant temperature pipe support includes the frame body with hollow interlayer, and multiple cylinders are arranged on vertical direction on the frame body and lead to
Road；The hollow interlayer connection water inlet pipe and outlet pipe；
Sand core plate, the liquid valve that bottom is arranged liquid outlet and is mounted on liquid outlet are set inside the Peptide systhesis pipe
Door；The outer diameter of the Peptide systhesis pipe is less than or equal to the inner diameter of the cylindrical channel of constant temperature pipe support；The polypeptide closes
At the fixed restraining position ring block in pipe outer wall upper end, the outer diameter of the stop collar is greater than the inner diameter of the cylindrical channel of constant temperature pipe support；
The waste collection pipe is the glass tube being arranged diagonally downward, and waste collection pipe upper end setting first is open,
Multiple waste collection mouths, the waste collection mouth and Peptide systhesis pipe is arranged in the second opening of lower end setting and third opening, side wall
Liquid outlet be connected；First opening is connected with nitrogen gas tank；Second opening is connected with waste collection bottle, and described second opens
Waste liquid valve is set at mouthful；The third opening is connected with vacuum pump.
As an improvement of the present invention, Peptide systhesis tube top portion setting sealing cover and the air guide across sealing cover
Pipe, the gas-guide tube access nitrogen separation device, and the nitrogen outlet of the nitrogen separation device accesses nitrogen by pipeline and collects
Bottle, the nitrogen receiving flask access waste collection pipe upper end by nitrogen circulation pipe.Nitrogen can be recycled, save at
This.
As an improvement of the present invention, pressure detector is set in the waste collection pipe.Pressure in detectable system
Power avoids pressure excessive, generates dangerous.
As an improvement of the present invention, the third opening setting and the second opening surface.Liquid is avoided to flow into true
Sky pump.
As an improvement of the present invention, the number of the Peptide systhesis pipe is 4-10.Number is more, the treatment effeciency moon
It is high.
Compared with the existing technology, advantages of the present invention is as follows,
High-throughput Solid phase peptide synthssis device of the invention, by the way that constant temperature pipe support is arranged, so that being fixed on inside pipe support
Peptide systhesis pipe is in temperature constant state, is conducive to the stabilization of Peptide systhesis, efficiently carries out, by the way that multiple Peptide systhesis pipes are arranged,
Multiple groups synthesis can be carried out simultaneously, improve production efficiency.
Detailed description of the invention
Fig. 1 is high-throughput Solid phase peptide synthssis schematic device；
Fig. 2 is the high-throughput Solid phase peptide synthssis schematic device of recyclable nitrogen；
In figure：1 it is constant temperature pipe support, 11 be frame body, 12 be cylindrical channel, 13 be water inlet pipe, 14 be outlet pipe, 2 is more
Peptide synthesis pipe, 21 be sand core plate, 22 be liquid outlet, 23 be liquid valve door, 24 be stop collar, 3 be waste collection pipe, 31 be first
Opening, 32 be the second opening, 33 be third opening, 34 be waste collection mouth, 35 be waste liquid valve, 36 be vacuum pump, 4 be nitrogen
Tank, 41 be sealing cover, 42 be gas-guide tube, 43 be nitrogen separation device, 44 be nitrogen receiving flask, 45 be nitrogen circulation pipe, 46 be
Pressure detector, 5 are waste collection bottles.
Specific embodiment
Embodiment 1：
Referring to Fig. 1-2, high-throughput Solid phase peptide synthssis device, including constant temperature pipe support 1, Peptide systhesis pipe 2, waste collection pipe
3, nitrogen gas tank 4 and waste collection bottle 5；
The constant temperature pipe support 1 includes the frame body 11 with hollow interlayer, and multiple circles are arranged on vertical direction on the frame body 11
Cylindrical passageway 12；The hollow interlayer connection water inlet pipe 13 and outlet pipe 14；
Sand core plate 21 is set inside the Peptide systhesis pipe 2, and bottom is arranged liquid outlet 22 and is mounted on liquid outlet 22
Liquid valve door 23；The inner wall that the outer diameter of the Peptide systhesis pipe 2 is less than or equal to the cylindrical channel 12 of constant temperature pipe support 1 is straight
Diameter；The fixed restraining position ring block 24 in the 2 outer wall upper end of Peptide systhesis pipe, the outer diameter of the stop collar 24 are greater than the circle of constant temperature pipe support 1
The inner diameter of cylindrical passageway 12；
The waste collection pipe 3 is the glass tube being arranged diagonally downward, 3 upper end of waste collection pipe setting, first opening
31,32 mouthfuls and third opening 33 are opened in lower end setting second, and side wall is arranged multiple waste collection mouths 34, the waste collection mouth 34 with
The liquid outlet 22 of Peptide systhesis pipe 2 is connected；First opening 31 is connected with nitrogen gas tank 4；Described second opens 32 mouthfuls receives with waste liquid
Collect bottle 5 to be connected, described second opens setting waste liquid valve 35 at 32 mouthfuls；The third opening 33 is connected with vacuum pump 36.
Embodiment 2：
Referring to Fig. 1-2, sealing cover 41 is set as an improvement of the present invention, at the top of the Peptide systhesis pipe 2 and is passed through
The gas-guide tube 42 of sealing cover 41, the gas-guide tube 42 access nitrogen separation device 43, and the nitrogen of the nitrogen separation device 43 goes out
Mouth accesses nitrogen receiving flask 44 by pipeline, and the nitrogen receiving flask 44 is accessed on waste collection pipe 3 by nitrogen circulation pipe 45
End.Nitrogen can be recycled, save the cost.
Embodiment 3：
Referring to Fig. 1-2, as an improvement of the present invention, pressure detector 46 is set in the waste collection pipe 3.It can examine
Pressure in survey system avoids pressure excessive, generates dangerous.
Embodiment 4：
Referring to Fig. 1-2, as an improvement of the present invention, 33 setting of third opening is opened right above 32 mouthfuls with second.
Liquid is avoided to flow into vacuum pump 36.
Embodiment 5：
Referring to Fig. 1-2, as an improvement of the present invention, the number of the Peptide systhesis pipe 2 is 4-10.Number is got over
More, the treatment effeciency moon is high.
The present invention can also be formed in combination with example 1 at least one of described technical characteristic of embodiment 2,3,4,5
New embodiment.
It should be noted that above-described embodiment is only presently preferred embodiments of the present invention, there is no for the purpose of limiting the invention
Protection scope, the equivalent substitution or substitution made on the basis of the above all belong to the scope of protection of the present invention.
Claims (5)
1. high-throughput Solid phase peptide synthssis device, which is characterized in that including constant temperature pipe support, Peptide systhesis pipe, waste collection pipe, nitrogen
Gas tank and waste collection bottle；
The constant temperature pipe support includes the frame body with hollow interlayer, and multiple cylindrical channels are arranged on the frame body on vertical direction；
The hollow interlayer connection water inlet pipe and outlet pipe；
Sand core plate, the liquid valve door that bottom is arranged liquid outlet and is mounted on liquid outlet are set inside the Peptide systhesis pipe；Institute
State Peptide systhesis pipe outer diameter be less than or equal to constant temperature pipe support cylindrical channel inner diameter；Outside the Peptide systhesis pipe
Restraining position ring block is fixed in wall upper end, and the outer diameter of the stop collar is greater than the inner diameter of the cylindrical channel of constant temperature pipe support；
The waste collection pipe is the glass tube being arranged diagonally downward, the first opening of waste collection pipe upper end setting, lower end
The second opening of setting and third opening, the multiple waste collection mouths of side wall setting, the waste collection mouth and Peptide systhesis pipe go out
Liquid mouth is connected；First opening is connected with nitrogen gas tank；Second opening is connected with waste collection bottle, second opening
Waste liquid valve is set；The third opening is connected with vacuum pump.
2. high throughput Solid phase peptide synthssis device as described in claim 1, which is characterized in that Peptide systhesis tube top portion is set
Sealing cover and the gas-guide tube across sealing cover are set, the gas-guide tube accesses nitrogen separation device, the nitrogen of the nitrogen separation device
Gas outlet accesses nitrogen receiving flask by pipeline, and the nitrogen receiving flask accesses waste collection pipe upper end by nitrogen circulation pipe.
3. high throughput Solid phase peptide synthssis device as described in claim 1, which is characterized in that setting in the waste collection pipe
Pressure detector.
4. high throughput Solid phase peptide synthssis device as described in claim 1, which is characterized in that the third opening setting and the
Right above two openings.
5. high throughput Solid phase peptide synthssis device as described in claim 1, which is characterized in that the number of the Peptide systhesis pipe
It is 4-10.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810631764.9A CN108912207A (en) | 2018-06-19 | 2018-06-19 | High-throughput Solid phase peptide synthssis device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810631764.9A CN108912207A (en) | 2018-06-19 | 2018-06-19 | High-throughput Solid phase peptide synthssis device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108912207A true CN108912207A (en) | 2018-11-30 |
Family
ID=64421979
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810631764.9A Pending CN108912207A (en) | 2018-06-19 | 2018-06-19 | High-throughput Solid phase peptide synthssis device |
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| Country | Link |
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| CN (1) | CN108912207A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109851657A (en) * | 2019-03-28 | 2019-06-07 | 东山腾新食品有限公司 | A kind of efficient high-flux polypeptide synthetic method and its device |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203269832U (en) * | 2013-03-14 | 2013-11-06 | 苏州强耀生物科技有限公司 | 96-channel semiautomatic polypeptide synthesizer |
| CN204122110U (en) * | 2014-09-29 | 2015-01-28 | 骏马化纤股份有限公司 | For the nitrogen cycle device of solid phase polycondensation |
| CN205088163U (en) * | 2015-11-06 | 2016-03-16 | 河南工程学院 | Controllable polypeptide solid -phase synthesis system of temperature |
| CN208776625U (en) * | 2018-06-19 | 2019-04-23 | 南京肽业生物科技有限公司 | High-throughput Solid phase peptide synthssis device |
-
2018
- 2018-06-19 CN CN201810631764.9A patent/CN108912207A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203269832U (en) * | 2013-03-14 | 2013-11-06 | 苏州强耀生物科技有限公司 | 96-channel semiautomatic polypeptide synthesizer |
| CN204122110U (en) * | 2014-09-29 | 2015-01-28 | 骏马化纤股份有限公司 | For the nitrogen cycle device of solid phase polycondensation |
| CN205088163U (en) * | 2015-11-06 | 2016-03-16 | 河南工程学院 | Controllable polypeptide solid -phase synthesis system of temperature |
| CN208776625U (en) * | 2018-06-19 | 2019-04-23 | 南京肽业生物科技有限公司 | High-throughput Solid phase peptide synthssis device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109851657A (en) * | 2019-03-28 | 2019-06-07 | 东山腾新食品有限公司 | A kind of efficient high-flux polypeptide synthetic method and its device |
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Application publication date: 20181130 |