CN108911857A - A kind of multifunctional slow-release organic fungi-manure - Google Patents
A kind of multifunctional slow-release organic fungi-manure Download PDFInfo
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- CN108911857A CN108911857A CN201810905294.0A CN201810905294A CN108911857A CN 108911857 A CN108911857 A CN 108911857A CN 201810905294 A CN201810905294 A CN 201810905294A CN 108911857 A CN108911857 A CN 108911857A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/30—Layered or coated, e.g. dust-preventing coatings
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/30—Layered or coated, e.g. dust-preventing coatings
- C05G5/37—Layered or coated, e.g. dust-preventing coatings layered or coated with a polymer
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Fertilizers (AREA)
Abstract
The present invention provides a kind of multifunctional slow-release organic fungi-manure, the organic fungi-manure includes organic matter, the bacterium of useful plants growth, dopamine or its salt, the high-polymer molecule, sodium alginate and Ca2+.Then the present invention coats the high-polymer molecule on dopamine surface, finally by sodium alginate and Ca by the way that dopamine to be attached to the surface of organic matter and beneficial bacterium2+It is coated on the high-polymer molecule surface, to prepare a kind of organic fungi-manure that can keep soil liquid manure and slow release viable bacteria to a certain extent.The present invention is sustained organic fungi-manure, soil liquid manure can effectively be kept and slow release viable bacteria improves the disease-resistant anti-adversity ability of crop to improve the utilization efficiency of liquid manure and viable bacteria, the improvement soil fertility of sustainability, and realize the efficient recycling of biological organic waste material.Sustained release organic fungi-manure provided by the invention, preparation process is simple and environmentally-friendly, does not use organic solvent, is suitable for industrial mass production application.
Description
Technical field
The invention belongs to fertilizer development technical fields, and in particular to a kind of multi-functional sustained release organic fungi-manure.
Background technique
Organic fertilizer refers to that, from plant or animal residue, plant nutrient is simultaneous to be effectively improved soil physical chemistry for providing
With the organic fertilizer of biological property.Organic fertilizer nutrient is comprehensive, by itself decomposable process can improving soil fertility, for plant growth
Development needs.
Bacterial manure can improve the nutritional condition of crop as a kind of bio-feritlizer by the vital movement of wherein microorganism
Accelerate absorption and conversion, inhibition harmful microbe activity of nutrient etc. such as the nitrogen in fixed air with nutrient supply situation
It is a large amount of in agricultural production to use.
Water and fertilizer are two key factors of plant growth, and soil moisture holding is agricultural and horticultural field compound fertilizer
One of most important index improves survival rate of seedling to soil quality is improved, and plant growth is promoted to have positive effect.Water and
Fertilizer, uncontrollable release cause liquid manure to be lost, application efficiency and utilization rate are low so that crop can not effectively absorb fertilizer.
Summary of the invention
The present invention provides a kind of multi-functional sustained release organic fungi-manures.The present invention by by dopamine be attached to organic matter and
Then the surface of beneficial bacterium coats the high-polymer molecule on dopamine surface, finally by sodium alginate and Ca2+It is coated on high polymer point
Sublist face, so that a kind of sustained release organic fungi-manure that can effectively keep soil liquid manure and slow release viable bacteria is prepared, to improve
The utilization efficiency of soil liquid manure and microbial inoculum.
It is an object of the present invention to provide a kind of organic fungi-manure, the organic fungi-manure includes organic matter, useful plants life
Long bacterium, dopamine or its salt, the high-polymer molecule, sodium alginate and Ca2+。
Specifically, the organic fungi-manure further includes following 1) -5) described at least one：
1) organic matter includes that the bacterium that can be grown for the useful plants provides the nutriment of its metabolism or breeding；
2) organic matter includes stalk, dead leaves and/or withered grass；
3) bacterium bag of the useful plants growth includes bacillus subtilis；
4) the high-polymer molecule includes superabsorbent water the high-polymer molecule；Specifically, the superabsorbent water the high-polymer molecule includes
The high-polymer molecule as made of acrylamide and acroleic acid polymerization；
5) dopamine or its salt are covered on the surface of the bacterium of the organic matter and/or useful plants growth, the height
Polymers molecule is covered on the dopamine or the surface of its salt；The sodium alginate and Ca2+The high-polymer molecule surface.
Specifically, the bacterial manure is prepared by the following method：
It is added in the Tris-HCl buffer of pH value 8~8.5 after organic matter crushed to the sieve of 500 mesh or more, it would be beneficial to
The bacterium of plant growth, which is added, to be mixed, and adds Dopamine hydrochloride, 12h or more is stirred at room temperature, filter surface is attached with dopamine
Organic fungi-manure particle；
The organic fungi-manure particle is placed in reaction vessel, ultrapure water is added, leads to N2, the high-polymer molecule is added into solution
Monomer, crosslinking agent, catalyst, be protected from light and continue logical N2, it stirs at room temperature, then reaction vessel is transferred in ice bath and is stirred,
Initiator is added, is transferred in closed mold, polymerize at room temperature for 24 hours after stirring, product be crushed into 200 meshes, obtain surface
Coat the organic bacteria fat particles of super Water-absorbing high polymer；
Water is added after sodium alginate is mixed with the organic bacteria fat particles that above-mentioned surface coats super Water-absorbing high polymer, obtains
Mixed solution；Mixed solution is added drop-wise to CaCl2In aqueous solution；It filters and is simultaneously rinsed with deionized water, dried to constant weight to obtain the final product.
It is a further object to provide a kind of preparation method of bacterial manure, the method includes：Organic matter is crushed
It being added in the Tris-HCl buffer of pH value 8~8.5 after crossing the sieve of 500 mesh or more, it would be beneficial to the bacterium of plant growth, which is added, to be mixed,
Add Dopamine hydrochloride, 12h or more be stirred at room temperature, filter surface is attached with the organic fungi-manure particle of dopamine；
The organic fungi-manure particle is placed in reaction vessel, ultrapure water is added, leads to N2, the high-polymer molecule is added into solution
Monomer, crosslinking agent, catalyst, be protected from light and continue logical N2, it stirs at room temperature, then reaction vessel is transferred in ice bath and is stirred,
Initiator is added, is transferred in closed mold, polymerize at room temperature for 24 hours after stirring, product be crushed into 200 meshes, obtain surface
Coat the organic bacteria fat particles of super Water-absorbing high polymer；
Water is added after sodium alginate is mixed with the organic bacteria fat particles that above-mentioned surface coats super Water-absorbing high polymer, obtains
Mixed solution；Mixed solution is added drop-wise to CaCl2In aqueous solution；It filters and is simultaneously rinsed with deionized water, dried to constant weight to obtain the final product.
Specifically, the method also includes following 1) -5) described at least one：
1) organic matter includes that the bacterium that can be grown for the useful plants provides the nutriment of its metabolism or breeding；
2) organic matter includes stalk, dead leaves, and/or withered grass；
3) bacterium bag of the useful plants growth includes bacillus subtilis；
4) the high-polymer molecule includes superabsorbent water the high-polymer molecule；Specifically, the superabsorbent water the high-polymer molecule includes
The high-polymer molecule as made of acrylamide and acroleic acid polymerization；
5) organic matter be crushed into 500 meshes.
Specifically, the method includes：
Stalk, dead leaves, withered grass etc. be crushed into 500 meshes, obtain debirs powder；Take debirs powder described in 50g
End plus 1L water are added 10g bacillus subtilis powder end, mix to obtain debirs bacterium solution；1.5g Tris is added in debirs bacterium
In liquid, the debirs bacterium solution pH value is adjusted with 6MHCl and obtains Tris-HCl buffer solution to 8~8.5.It is more that 5g hydrochloric acid is added
Bar amine, is stirred at room temperature 12h or more, filter surface package is attached with the organic fungi-manure particle of dopamine；The organic fungi-manure is micro-
Grain is made of the debirs powder and bacillus subtilis；
1-5g organic fungi-manure particle is placed in the reaction vessel of 100ml, 50ml ultrapure water is added；Logical N20.5h removes it
In dissolved oxygen, be added into solution by a certain amount of monomeric acrylamide (AM), acrylic acid (AA), crosslinking agent Al (OH)3NPs
And catalyst TEMED, dosage are respectively AA 2.16g/100g H2O, AM19.1g/100g H2O、Al(OH)3NPs 5g/
100gH28 μ L/ of O and TEMED] 00gH2O is protected from light under tinfoil cladding and continues logical N2, quick magnetic agitation 15min at room temperature；
Then reaction vessel is transferred in ice bath and is slowly stirred 5min, initiator KPS is added, and (KPS dosage is 2g/100g H2O), stir
It is transferred quickly in closed mold, polymerize at room temperature for 24 hours after mixing 30s；Finally product is crushed, successively uses deionized water and second
Alcohol washing, room temperature are dried to constant weight, and by 200 meshes, obtain the organic bacteria fat particles that surface coats super Water-absorbing high polymer；
In mass ratio 1: 0.5~1: 2, the organic bacteria of super Water-absorbing high polymer is coated by sodium alginate and with above-mentioned surface
Fat particles mixing, takes 1-5g mixture to be added in 50ml water, obtains mixed solution；Mixed solution is slowly dropped to 0.5~1wt%
CaCl2In aqueous solution；The volume ratio of mixed solution and calcium chloride water is 1: 4；Calcium chloride crosslinker solution is filtered and is used in combination
Deionized water is rinsed to remove excessive calcium chloride, then dries product to constant weight at 37 DEG C to obtain the final product；
In the method, method after each compound is zoomed in or out together in proportion, also in protection model of the invention
Within enclosing.
Of the invention a further object is provides the present invention organic fungi-manure that any the method is prepared.
It is also another object of the present invention to provide the applications of any organic fungi-manure of the present invention.
Specifically, the application includes following 1) -4) described at least one：
1) sustained release or controlled release product as the bacterium of useful plants growth；
2) product as useful plants growth；
3) as Ecological Distribution of Soil Microorganisms or the adjusting product of nutrition condition；
4) as the sustained release of fertilizer or controlled release product.
The plant includes crops；
The bacterium bag of the useful plants growth includes bacillus subtilis.
It is also another object of the present invention to provide the applications of any the method for the present invention.
Specifically, the application includes following 1) -4) described at least one：
1) application in the product or its Related product that preparation has the bacterium of sustained release or the growth of controlled release useful plants；
2) application in the product or its Related product that preparation has useful plants growth；
3) there is the application in the product or its Related product for adjusting Ecological Distribution of Soil Microorganisms or nutrition condition in preparation；
4) application in the product or its Related product that preparation has sustained release or release and release control fertilizer.
The plant includes crops；
One kind provided by the invention, has at least the following advantages：
(1) present invention sustained release organic fungi-manure, by cladding organic matter and beneficial microbe, to improve the nutritional condition of crop
With nutrient supply situation, inhibit harmful microbe activity, to improve the disease-resistant anti-adversity ability of crop；In addition, organic matter is not
It only can be used as fertilizer in itself, realize refuse reclamation, additionally it is possible to promote beneficial microorganism to breed, beneficial microorganism is largely fast
Speed breeding can promote the degradation of organic matter and the release of nutrient again in turn, improve the utilization efficiency of organic matter.
(2) sustained release organic fungi-manure provided by the invention, preparation process is simple and environmentally-friendly, does not use organic solvent, is suitable for
Industrial mass production application.
(3) present invention sustained release organic fungi-manure, can effectively keep soil liquid manure and slow release viable bacteria, specific real at one
It applies in mode, high polymer of the present invention has selected super absorbent polymer, which can be absorbed greater than tens times of its weight to several
Thousand times of water prepares slow-release compound fertilizer, Ke Yiyou in the super absorbent polymer (AA-co-AM) of organic fungi-manure surface coating
The moisture and nutrient of effect saved in soil improves soil moisture to slow down water evaporation rate and nutrient release rate
With the utilization efficiency of nutrient.
(4) in a specific embodiment, with the raising of temperature, the super absorbent polymer that the present invention selects is shown
The enhancing of water absorbing capacity, to achieve the purpose that the variation intelligent control fertilizer performance according to environment, to slow down 35 DEG C or more
Under hot environment, the rapid evaporation and release of nutrient, moisture, improve the utilization rate of liquid manure in soil and fertilizer.In addition, with temperature
Raising, super absorbent polymer shows the decline of water holding capacity, this performance is conducive under hot environment, intelligent control sustained release
Organic fungi-manure discharges a certain amount of liquid manure, reduces the temperature of crop ambient enviroment, and crop is helped to resist hot environment.
(5) sodium alginate and Ca2+It is cross-linked to form three-dimensional netted " eggshell " structure, wraps up coating superabsorbent water polymerization therein
The organic fungi-manure kernel of object achievees the purpose that slow release fertilizer using the variation of inside and outside osmotic pressure.As super absorbent polymer absorbs water
Swelling, " eggshell " inside water pressure are also gradually increased, when " eggshell " inside water pressure is greater than the hydraulic pressure of external soil or environment, in
The organic fungi-manure kernel of absorbed water dissolution is discharged into outside with water, and a part of organic fungi-manure kernel of release is by soil
Parsing utilizes, and the hydraulic pressure of external soil increases, and high polymer again wraps up the water in soil, that is, organic fungi-manure kernel absorption into seaweed
Sour sodium and Ca2+It is cross-linked to form in three-dimensional netted " eggshell " structure, repeatedly, achievees the purpose that slow release liquid manure.
Specific embodiment
Made in following embodiments, experimental method is conventional method unless otherwise specified.
Material as used in the following examples etc., is commercially available unless otherwise specified.
Following embodiments and its illustrate for explanation and understanding the present invention, do not constitute to improper limit of the invention
It is fixed.
The preparation of embodiment 1, multifunctional slow-release fertilizer
(1) surface package is attached with the preparation of the organic fungi-manure particle of dopamine
Stalk, dead leaves, withered grass etc. be crushed into 500 meshes, obtain debirs powder；Take debirs powder described in 50g
End plus 1L water are added 10g bacillus subtilis powder end, mix to obtain debirs bacterium solution；1.5g Tris is added in debirs bacterium
In liquid, the debirs bacterium solution pH value is adjusted with 6MHCl and obtains Tris-HCl buffer solution to 8~8.5.It is more that 5g hydrochloric acid is added
Bar amine, is stirred at room temperature 12h or more, filter surface package is attached with the organic fungi-manure particle of dopamine, the organic fungi-manure is micro-
Grain is made of the debirs powder and bacillus subtilis.
(2) superabsorbent water the high-polymer molecule material (AA-co-AM) is coated in organic fungi-manure microparticle surfaces
1-5g organic fungi-manure particle is placed in the reaction vessel of 100ml, 50ml ultrapure water is added；Logical N20.5h removes it
In dissolved oxygen, be added into solution by a certain amount of monomeric acrylamide (AM), acrylic acid (AA), crosslinking agent Al (OH)3NPs
And catalyst TEMED, dosage are respectively AA 2.16g/100g H2O, AM19.1g/100g H2O、Al(OH)3NPs 5g/
100gH28 μ L/100gH of O and TEMED2O is protected from light under tinfoil cladding and continues logical N2, quick magnetic agitation 15min at room temperature.
Then reaction vessel is transferred in ice bath and is slowly stirred 5min, initiator KPS is added, and (KPS dosage is 2g/100g H2O), stir
It is transferred quickly in closed mold, polymerize at room temperature for 24 hours after mixing 30s.Finally product is crushed, successively uses deionized water and second
Alcohol washing, room temperature are dried to constant weight, and by 200 meshes, obtain the organic bacteria fat particles that surface coats super Water-absorbing high polymer.
(3) it is sustained the preparation of organic fungi-manure
In mass ratio 1: 0.5~1: 2, sodium alginate and above-mentioned surface are coated to the organic fungi-manure of super Water-absorbing high polymer
Grain mixing, takes 1-5g mixture to be added in 50ml water, obtains mixed solution；Mixed solution is slowly dropped to 0.5~1wt%'s
CaCl2In aqueous solution.The volume ratio of mixed solution and calcium chloride water is 1: 4.After a period of time, calcium chloride is crosslinked molten
Liquid filtering is simultaneously rinsed with deionized water to remove excessive calcium chloride, is then dried product to constant weight at 37 DEG C.
The performance of embodiment 2, multi-functional sustained release organic fungi-manure
(1) water suction and water retention property test of organic fungi-manure are sustained
The multi-functional sustained release organic fungi-manure prepared according to 1 the method for embodiment is taken, tests 15 DEG C, 25 DEG C, 35 respectively
DEG C when, the sustained release organic fungi-manure is to the water retention after the absorbability (g/g) and 12h of 0.9wt%NaC1 aqueous solution
(wt%), test result is as shown in table 1.
Table 1
Table 1 the result shows that, 1 the method for embodiment preparation multi-functional sustained release organic fungi-manure, water suction, water retention property it is excellent
Different, and as the temperature rises, water absorbing capacity is in rising trend, and therefore, which can effectively avoid soil, in fertilizer
Liquid manure excessive evaporation or release, especially at temperature higher summer and noon, so, which is suitble to early
The application of rice, winter wheat and the biggish crop planting area of the morning and evening temperature difference.It is provided by the invention slow at noon summer of high temperature
Releasing organic fungi-manure can also automatically save to a certain extent in soil and its water of package, fertilizer, viable bacteria are not lost, and keep the soil water
Fertilizer, the utilization efficiency for improving fertilizer, microbial inoculum.
In addition, as the temperature rises, the water absorbing capacity of the multi-functional sustained release organic fungi-manure of 1 the method for embodiment preparation
While power is in rising trend, water holding capacity is on a declining curve, and it is synthermal that water absorbing capacity rising is conducive to sustained release organic fungi-manure
Liquid manure evaporation rate caused by increasing accelerates to compete, and reduces the evaporation waste of liquid manure, meanwhile, sustained release organic fungi-manure is retained energy
The decline of power is conducive to be sustained organic fungi-manure slow-released part moisture and organic fungi-manure into soil, crop periphery is effectively reduced
Temperature is conducive to plant resistant hot environment.
(2) viable bacteria release experiment
The multi-functional sustained release organic fungi-manure 6g prepared according to 1 the method for embodiment is taken, is equally divided into 3 parts, respectively at
15 DEG C, 25 DEG C, place 10 days in 35 DEG C of environment (humidity keeps 40% or less), then detect different temperatures different standing times
Under, it is sustained the viable bacteria burst size of organic fungi-manure.
Plate detection method detects viable bacteria and discharges quantity measuring method：
1) in 1.2 micron membrane filter of suction funnel middle berth, the sample after placement is placed on filter membrane, is filtered, side is taken out when suction filtration
Vacuum side is into funnel plus sterile water, the total volume of sterile water are 10 times of sample quality；The step does not discharge viable bacteria for filtering off
Surface package be attached with the sustained release organic fungi-manure of sodium alginate and the high-polymer molecule；
2) collect filtrate, be added bead, stand 20min, on rotary shaker 200r/min vibrate 30min to get
Mother liquor bacteria suspension；
3) it draws the above-mentioned mother liquor bacteria suspension of 5.0ml respectively with Sterile pipette to be added in 45ml sterile water, is by volume 1
: 10 ratio is serially diluted, and a series of dilution bacteria suspensions of different extension rates are respectively obtained；
4) each sample takes 3 dilutions for being continuously suitable for, and draws different dilution bacteria suspensions respectively with Sterile pipette
0.1ml is added on solid agar medium, and the bacteria suspension of different dilutions is uniformly applied to solid with sterile glass scraper respectively
On agar medium；Each dilution is repeated 3 times, while making blank control with sterile water, is trained in 37 DEG C of constant incubator
Support 12-36h；
5) bacterium colony is counted calculates with viable count purpose in sample：According to national standards the method in GB20287-2006 into
Row.
Testing result is as shown in table 2.Table 2 the result shows that, the sustained release organic bacteria being prepared according to 1 the method for embodiment
Fertilizer can effectively discharge viable bacteria, and as the temperature rises, and the burst size of viable bacteria also gradually increases, but rate of release is to slow down
's.This is the experimental results showed that sustained release organic fungi-manure provided by the invention is very suitable to the fertilising of early rice and winter wheat.And in height
The summer of temperature, sustained release organic fungi-manure provided by the invention can slow down the release of the viable bacteria of its package to a certain degree.Because of crop at this time
Slow growth itself does not need excessive fertilizer, and sustained release organic fungi-manure viable bacteria rate of release provided by the invention slows down, centainly
It is exactly the utilization efficiency for improving viable bacteria in degree.
Table 2
Embodiments of the present invention above described embodiment only expresses, the description thereof is more specific and detailed, but can not
Therefore limitations on the scope of the patent of the present invention are interpreted as, as long as skill obtained in the form of equivalent substitutions or equivalent transformations
Art scheme should all be fallen within the scope and spirit of the invention.
Claims (10)
1. a kind of organic fungi-manure, which is characterized in that the organic fungi-manure includes organic matter, the bacterium of useful plants growth, dopamine
Or its salt, the high-polymer molecule, sodium alginate and Ca2+。
2. organic fungi-manure according to claim 1, which is characterized in that the organic fungi-manure further includes following 1) -5) in institute
At least one stated：
1) organic matter includes that the bacterium that can be grown for the useful plants provides the nutriment of its metabolism or breeding；
2) organic matter includes stalk, dead leaves and/or withered grass；
3) bacterium bag of the useful plants growth includes bacillus subtilis；
4) the high-polymer molecule includes superabsorbent water the high-polymer molecule；Specifically, the superabsorbent water the high-polymer molecule includes by third
The high-polymer molecule made of acrylamide and acroleic acid polymerization；
5) dopamine or its salt are covered on the surface of the bacterium of the organic matter and/or useful plants growth, the high polymer
Molecule is covered on the dopamine or the surface of its salt；The sodium alginate and Ca2+The high-polymer molecule surface.
3. according to claim 1 and/or bacterial manure described in 2, which is characterized in that the bacterial manure is prepared by the following method：
It is added in the Tris-HCl buffer of pH value 8~8.5 after organic matter crushed to the sieve of 500 mesh or more, it would be beneficial to plant
The bacterium of growth, which is added, to be mixed, and adds Dopamine hydrochloride, 12h or more is stirred at room temperature, filter surface is attached with having for dopamine
Machine bacterial manure particle；
The organic fungi-manure particle is placed in reaction vessel, ultrapure water is added, leads to N2, the list of the high-polymer molecule is added into solution
Body, crosslinking agent, catalyst are protected from light and continue logical N2, stir at room temperature, then reaction vessel be transferred in ice bath and is stirred, be added
Initiator, is transferred in closed mold after stirring, polymerize at room temperature for 24 hours, and product crushed 200 meshes, obtains surface cladding
The organic bacteria fat particles of super Water-absorbing high polymer；
Water is added after sodium alginate is mixed with the organic bacteria fat particles that above-mentioned surface coats super Water-absorbing high polymer, is mixed
Solution；Mixed solution is added drop-wise to CaCl2In aqueous solution；It filters and is simultaneously rinsed with deionized water, dried to constant weight to obtain the final product.
4. a kind of preparation method of bacterial manure, which is characterized in that the method includes：Organic matter crushed to the sieve of 500 mesh or more
It is added in the Tris-HCl buffer of pH value 8~8.5 afterwards, it would be beneficial to which the bacterium of plant growth, which is added, to be mixed, and hydrochloric acid DOPA is added
12h or more is stirred at room temperature in amine, filter surface is attached with the organic fungi-manure particle of dopamine；
The organic fungi-manure particle is placed in reaction vessel, ultrapure water is added, leads to N2, the list of the high-polymer molecule is added into solution
Body, crosslinking agent, catalyst are protected from light and continue logical N2, stir at room temperature, then reaction vessel be transferred in ice bath and is stirred, be added
Initiator, is transferred in closed mold after stirring, polymerize at room temperature for 24 hours, and product crushed 200 meshes, obtains surface cladding
The organic bacteria fat particles of super Water-absorbing high polymer；
Water is added after sodium alginate is mixed with the organic bacteria fat particles that above-mentioned surface coats super Water-absorbing high polymer, is mixed
Solution；Mixed solution is added drop-wise to CaCl2In aqueous solution；It filters and is simultaneously rinsed with deionized water, dried to constant weight to obtain the final product.
5. according to the method described in claim 4, it is characterized in that, the method also includes following 1) -5) described at least
It is a kind of：
1) organic matter includes that the bacterium that can be grown for the useful plants provides the nutriment of its metabolism or breeding；
2) organic matter includes stalk, dead leaves, and/or withered grass；
3) bacterium bag of the useful plants growth includes bacillus subtilis；
4) the high-polymer molecule includes superabsorbent water the high-polymer molecule；Specifically, the superabsorbent water the high-polymer molecule includes by third
The high-polymer molecule made of acrylamide and acroleic acid polymerization；
5) organic matter be crushed into 500 meshes.
6. the organic fungi-manure being prepared by claim 4 and/or 5 the methods.
7. the application of organic fungi-manure described in any organic fungi-manure of claim 1-3 and/or claim 6.
8. application according to claim 7, which is characterized in that the application includes following 1) -4) described at least one
Kind：
1) sustained release or controlled release product as the bacterium of useful plants growth；
2) product as useful plants growth；
3) as Ecological Distribution of Soil Microorganisms or the adjusting product of nutrition condition；
4) as the sustained release of fertilizer or controlled release product.
9. the application of claim 4 and/or 5 the methods.
10. application according to claim 9, which is characterized in that the application includes following 1) -4) described at least one
Kind：
1) application in the product or its Related product that preparation has the bacterium of sustained release or the growth of controlled release useful plants；
2) application in the product or its Related product that preparation has useful plants growth；
3) there is the application in the product or its Related product for adjusting Ecological Distribution of Soil Microorganisms or nutrition condition in preparation；
4) application in the product or its Related product that preparation has sustained release or release and release control fertilizer.
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| Publication number | Priority date | Publication date | Assignee | Title |
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