CN108905630A - A kind of rapid serum extractor - Google Patents
A kind of rapid serum extractor Download PDFInfo
- Publication number
- CN108905630A CN108905630A CN201811003529.3A CN201811003529A CN108905630A CN 108905630 A CN108905630 A CN 108905630A CN 201811003529 A CN201811003529 A CN 201811003529A CN 108905630 A CN108905630 A CN 108905630A
- Authority
- CN
- China
- Prior art keywords
- blood
- serum
- cylinder type
- hollow shell
- separation membrane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
Abstract
The present invention relates to a kind of rapid serum extractors, it is characterised in that is made of cylinder type hollow shell (1), sealing piston (2), sampling head (3) and blood separation membrane piece (4)；Wherein cylinder type hollow shell (1) is serum collection main body, and the upper end is inserted by sealing piston (2) and guarantees air-tightness, and lower end is connected with sampling head (3)；Blood separation membrane piece (4) is fixed on cylinder type hollow shell (1) bottom.Utilize the technology of UF membrane, vacuum power is generated by sampling head (3) by drawing sealing piston (2), and whole blood is pumped into cocurrent through blood Separation membrane (4), using Separation membrane (4) nanoscale duct in blood haemocyte and fibrinogen shut off, only serum is allowed to reach cylinder type hollow shell (1) through diaphragm, completes the acquisition of serum.The apparatus structure is simple, and it is convenient to operate, and can realize the extraction of pure serum in whole blood as quick as thought, substantially increase the efficiency of medical diagnosis on disease in hospital, provides the technological means of a kind of blood preseration and sample backtracking.
Description
Technical field
The present invention relates to a kind of rapid serum extractor, which uses seperation film for main element, passes through the sieve of film
It is allocated as with being completely removed leucocyte, red blood cell, blood platelet and the fibrinogen in whole blood and do not destroy eucaryotic cell structure to mention
Pure serum is taken out, the analysis and long term storage for blood.
Background technique
In modern medical service, the inspection of blood is one of most important technology of clinical diagnosis.Generally, due to the self-solidifying of blood
Mechanism, relevant biochemical analysis are required to extract serum from blood as analysis sample, meanwhile, serum after extraction compared to
Whole blood, storage time will greatly extend, the backtracking of convenience sample.Currently, the serum separation method generally used in hospital is
Each component is layered by different revolving speeds and is collected based on the quality difference of each component in blood by centrifugal filtration.However, blood
Haemocyte in liquid is very fragile, and the breakage of cell wall easily occurs at high speed, causes haemolysis, but the slow-speed of revolution
It is difficult for each group to be kept completely separate again.Therefore, quick, the lossless and thorough serum extractive technique of separation will be real with important medical treatment
Trample meaning and large market prospect.
Membrane separation technique is a kind of emerging isolation technics in situ, and efficiently, the characteristic of low energy consumption and low cost becomes
The Hot spots for development of separation field at present.It is applied at present in the existing part commercialization achievement of medical field, such as Rend dialysis.So
And the use of most of film is required based on finished Components and device, at present on the market still not accordingly based on membrane technology
Serum extraction element.As can developing a kind of inexpensive, high-throughput membrane type serum extractor, then traditional centrifugation skill can be solved
The quick nondestructive of the defects of time-consuming for art, easy haemolysis, the separation in situ and serum of realizing blood extracts, and dramatically speeds up blood testing
Period improves the accuracy of detection.
Summary of the invention
The object of the present invention is to provide a kind of rapid serum extractor, a kind of serum of the rapidly extracting serum from whole blood is mentioned
Device is taken, to improve the long-term preservability of the efficiency of blood testing and blood sample in clinical treatment.The serum extractor structure is simple, at
This it is lower, it can be achieved that manually and be automatically brought into operation, whole blood sample is separated immediately suitable for blood collection procedure, remove blood in
Red blood cell, leucocyte, blood platelet and fibrinogen, extract pure serum.The device is easy to operate, it is easy to clean and
Disinfection has good medical prospect.
The technical scheme is that：A kind of rapid serum extractor, it is characterised in that by cylinder type hollow shell 1, close
Piston 2, sampling head 3 and blood separation membrane piece 4 is sealed to form；Wherein cylinder type hollow shell 1 be serum collection main body, the upper end by
Sealing piston 2 is inserted into and guarantees air-tightness, and lower end is connected with sampling head 3；Blood separation membrane piece 4 is fixed on cylinder type hollow shell
The diameter of 1 bottom of body, blood separation membrane piece 4 is identical as the internal diameter of cylinder type hollow shell 1, it is ensured that it is closely stuck in shell bottom
Portion.Vacuum power is generated by sampling head by drawing sealing piston, whole blood is pumped into cocurrent through blood Separation membrane, utilizes seperation film
Piece nanoscale duct in blood haemocyte and fibrinogen shut off, only allow serum through diaphragm reach cylinder type hollow
Shell completes the acquisition of serum.
It is preferred that the material of the cylinder type hollow shell 1 is glass, plastics, resin or ceramics.The cylindrical empty
1 diameter of heart shell is 1-3cm, length 4-10cm；The sealing piston 2 is matched with 1 size of cylinder type hollow shell to protect
Air-tightness is demonstrate,proved, while can be plugged up and down.
It is preferred that the material of sampling head 3 is metal, glass, plastics, resin or ceramics.It is preferred that the material of blood separation membrane piece 4
For：Ceramics, stainless steel, gold, silver, polysulfones, polyether sulfone, polyethylene, polypyrrole, dopamine, graphene or carbon nanotube.Sampling head
3 are connected 1 with cylinder type hollow shell.
It is preferred that the diameter of blood separation membrane piece 4 is 1-3cm, with a thickness of 0.1-1cm.4 structure of blood separation membrane piece is chip
Film, membrane aperture 100-500nm.Blood separation membrane piece 4 is fixed on cylinder type hollow shell lower end, not mobile with sealing piston.
The extractor can be using the technology of UF membrane to red blood cell, leucocyte, blood platelet and the fiber in fresh blood
Proteinogen shuts off, and only allows serum through the duct of film and is collected, and the rapid serum that can be used for clinical blood chemical examination mentions
It takes and the long-time storage of blood sample.When work, make to generate negative pressure formation draft in shell by the twitch of sealing piston, it will
Blood sucks from sampling head and contacts blood parting surface membrane surface, since the pore size of Separation membrane will be lower than haemocyte and fibre
Fibrillarin is former, sieves principle according to size, these blood constitutents are trapped in membrane surface, and smaller serum then can be fast
Speed penetrates fenestra, reaches enclosure interior and is collected storage.
Beneficial effect：
The present invention is based on membrane separation technique, a kind of quick, lossless serum extractor is designed and prepared.The device is with blood
Seperation film is core component, by nanoscale UF membrane duct by red blood cell, leucocyte, blood platelet and the fiber egg in whole blood
White original is all shut off, and pure serum is only allowed to be collected by fenestra road.The apparatus structure is simple, operation is convenient, and by
In in situ, the lossless stalling characteristic using film, can anti-Hemostatic Oral Liquid cause the breakage of haemocyte, obtained blood during the separation process
Pure cleanliness is high, pollution-free, substantially increases the efficiency of medical diagnosis on disease in hospital, provides a kind of blood preseration and sample backtracking
Technological means.
Detailed description of the invention
Fig. 1 is a kind of structure drawing of device of rapid serum extractor, in figure：1- cylinder type hollow shell；2- sealing piston；
3- sampling head；4- blood separation membrane piece.
Specific embodiment
Embodiment 1
As shown in Figure 1：A kind of rapid serum extractor, by cylinder type hollow shell 1, sealing piston 2, sampling head 3 and blood
Liquid Separation membrane 4 forms；Wherein cylinder type hollow shell 1 is serum collection main body, and the upper end is inserted into and is guaranteed by sealing piston 2
Air-tightness, lower end are connected with sampling head 3；Blood separation membrane piece 4 is fixed on 1 bottom of cylinder type hollow shell, blood separation membrane
The diameter of piece 4 is identical as the internal diameter of cylinder type hollow shell 1, it is ensured that it is closely stuck in housing bottom.Pass through drawing sealing piston
Vacuum power is generated by sampling head, whole blood is pumped into cocurrent through blood Separation membrane, since the size of haemocyte in blood is all larger than 2 μ
M, thus using Separation membrane nanoscale duct in blood haemocyte and fibrinogen shut off, only allow serum penetrate film
Piece reaches cylinder type hollow shell, completes the acquisition of serum.
The working method of the serum extractor is sealing piston 2 to be inserted into cylinder type hollow shell 1, and be pushed into bottom, will
In the new blood that the insertion of sampling head 3 is extracted, drawing sealing piston 2 generates negative pressure, and blood is pumped into cylinder type hollow shell 1,
And contact blood separation membrane piece 4 in blood haemocyte and fibrinogen shut off, remaining serum passed through blood
Separation membrane 4 reaches storage in 1 cavity of cylinder type hollow shell.
Embodiment 2
The device of the invention is with embodiment 1, to the preferred ruler of one of which of blood separated in synchronization verifying attachment of the invention
Very little and material is described in detail.The material of cylinder type hollow shell 1 is glass, a diameter of 1cm, length 4cm；Sampling head
3 material is stainless steel；The material of blood separation membrane piece 4 is aluminium oxide, diameter 1cm, with a thickness of 0.1cm.Blood separation membrane
4 structure of piece is chip film, membrane aperture 500nm.
Embodiment 3
The device of the invention is with embodiment 1, to the preferred ruler of one of which of blood separated in synchronization verifying attachment of the invention
Very little and material is described in detail.The material of cylinder type hollow shell 1 is shellac, a diameter of 3cm, length 10cm；Sampling
First 3 material is zirconia ceramics；The material of blood separation membrane piece 4 is polyether sulfone, diameter 3cm, with a thickness of 1cm.Blood point
It is chip film, membrane aperture 10nm from 4 structure of diaphragm.
Embodiment 4
The device of the invention is with embodiment 1, to the preferred ruler of one of which of blood separated in synchronization verifying attachment of the invention
Very little and material is described in detail.The material of cylinder type hollow shell 1 is aluminium oxide ceramics, a diameter of 2cm, length 6cm；
The material of sampling head 3 is polyethylene；The material of blood separation membrane piece 4 is stainless steel, diameter 2cm, with a thickness of 0.5cm.Blood
4 structure of Separation membrane is chip film, membrane aperture 100nm.
Above-described embodiment 2-4, using whole blood as raw material, it is as follows that the serum extracted extracts performance：
| Red blood cell content in serum | 0 |
| Leucocyte content in serum | 0 |
| Platelet content in serum | 0 |
| Hemoglobin content in serum | 0g/L |
| Detect relative standard deviation | <5% |
Four embodiments described above are only the preferred embodiment of the present invention, before not departing from the technology of the present invention principle
It puts, being correspondingly improved and replacing also should be regarded as protection scope of the present invention.
Claims (7)
1. a kind of rapid serum extractor, it is characterised in that by cylinder type hollow shell (1), sealing piston (2), sampling head (3)
It is formed with blood separation membrane piece (4)；Wherein cylinder type hollow shell (1) is serum collection main body, and the upper end is by sealing piston (2)
It is inserted into and guarantees air-tightness, lower end is connected with sampling head (3)；Blood separation membrane piece (4) is fixed on cylinder type hollow shell (1)
Bottom.
2. rapid serum extractor according to claim 1, it is characterised in that：The cylinder type hollow shell (1)
Material is glass, plastics, resin or ceramics.
3. rapid serum extractor according to claim 1, it is characterised in that：The cylinder type hollow shell (1) is straight
Diameter is 1-3cm, length 4-10cm.
4. rapid serum extractor according to claim 1, it is characterised in that：The material of sampling head (3) is metal, glass
Glass, plastics, resin or ceramics.
5. rapid serum extractor according to claim 1, it is characterised in that：The material of blood separation membrane piece (4) is：Pottery
Porcelain, stainless steel, gold, silver, polysulfones, polyether sulfone, polyethylene, polypyrrole, dopamine, graphene or carbon nanotube.
6. rapid serum extractor according to claim 1, it is characterised in that：The diameter of blood separation membrane piece (4) is 1-
3cm, with a thickness of 0.1-1cm.
7. blood separated in synchronization verifying attachment according to claim 1, it is characterised in that：Blood separation membrane piece (4) structure
For chip film, membrane aperture 100-500nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811003529.3A CN108905630A (en) | 2018-08-30 | 2018-08-30 | A kind of rapid serum extractor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811003529.3A CN108905630A (en) | 2018-08-30 | 2018-08-30 | A kind of rapid serum extractor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108905630A true CN108905630A (en) | 2018-11-30 |
Family
ID=64406390
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811003529.3A Pending CN108905630A (en) | 2018-08-30 | 2018-08-30 | A kind of rapid serum extractor |
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| CN (1) | CN108905630A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0763769A (en) * | 1993-08-24 | 1995-03-10 | Asahi Fiber Glass Co Ltd | Method and device for removing solid component from humor |
| JP2004344874A (en) * | 2002-11-19 | 2004-12-09 | Sekisui Chem Co Ltd | Separation membrane of plasma or serum and filter apparatus using separation membrane of plasma or serum |
| CN1714292A (en) * | 2002-11-19 | 2005-12-28 | 积水化学工业株式会社 | Plasma or serum separation membrane and filter apparatus including the plasma or serum separation membrane |
| JP2007333578A (en) * | 2006-06-15 | 2007-12-27 | Sekisui Chem Co Ltd | Blood separation member |
| CN105307758A (en) * | 2013-06-27 | 2016-02-03 | 曼·胡默尔有限公司 | Ceramic whole blood hollow fiber membrane filter medium and use thereof for separating blood plasma / serum from whole blood |
| CN209549188U (en) * | 2018-08-30 | 2019-10-29 | 南京工业大学 | A kind of rapid serum extractor |
-
2018
- 2018-08-30 CN CN201811003529.3A patent/CN108905630A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0763769A (en) * | 1993-08-24 | 1995-03-10 | Asahi Fiber Glass Co Ltd | Method and device for removing solid component from humor |
| JP2004344874A (en) * | 2002-11-19 | 2004-12-09 | Sekisui Chem Co Ltd | Separation membrane of plasma or serum and filter apparatus using separation membrane of plasma or serum |
| CN1714292A (en) * | 2002-11-19 | 2005-12-28 | 积水化学工业株式会社 | Plasma or serum separation membrane and filter apparatus including the plasma or serum separation membrane |
| JP2007333578A (en) * | 2006-06-15 | 2007-12-27 | Sekisui Chem Co Ltd | Blood separation member |
| CN105307758A (en) * | 2013-06-27 | 2016-02-03 | 曼·胡默尔有限公司 | Ceramic whole blood hollow fiber membrane filter medium and use thereof for separating blood plasma / serum from whole blood |
| CN209549188U (en) * | 2018-08-30 | 2019-10-29 | 南京工业大学 | A kind of rapid serum extractor |
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