CN108902947A - A kind of Jerusalem artichoke probiotics ferment and preparation method thereof - Google Patents

A kind of Jerusalem artichoke probiotics ferment and preparation method thereof Download PDF

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Publication number
CN108902947A
CN108902947A CN201810806059.8A CN201810806059A CN108902947A CN 108902947 A CN108902947 A CN 108902947A CN 201810806059 A CN201810806059 A CN 201810806059A CN 108902947 A CN108902947 A CN 108902947A
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China
Prior art keywords
fermentation
jerusalem artichoke
probiotics
ferment
preparation
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CN201810806059.8A
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Chinese (zh)
Inventor
李恒
李中洋
李�雨
王春阳
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Yang Sheng (nan Zhao) Biological Technology Co Ltd
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Yang Sheng (nan Zhao) Biological Technology Co Ltd
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Priority to CN201810806059.8A priority Critical patent/CN108902947A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof

Abstract

The invention belongs to ferment production technical fields, and in particular to a kind of Jerusalem artichoke probiotics ferment and preparation method thereof.The present invention is using Jerusalem artichoke, dragon fruit, hawthorn, Chinese yam as primary raw material, by fermenting three times.The nutritional ingredient of raw material of the present invention is more abundant in scientific and reasonable collocation, under the fermentation of probiotics, the type, dosage of strict control probiotics and fermentation duration simultaneously, guarantee the fermentation of raw material more sufficiently, depth, finally obtain a kind of good mouthfeel, Jerusalem artichoke probiotics ferment that can be hypoglycemic.

Description

A kind of Jerusalem artichoke probiotics ferment and preparation method thereof
Technical field
The invention belongs to ferment production technical fields, and in particular to a kind of Jerusalem artichoke probiotics ferment and preparation method thereof.
Background technique
Ferment, which refers to, ferments to fruit and vegetable by microorganism, a kind of liquid containing bioactive ingredients of extraction.Above-mentioned life Object active constituent includes enzyme but is not limited to enzyme, including active antioxidants, biomass element etc., from fermentation participate in bacterium and Food materials for fermentation.
The ferment rationally made, the active substance of plant extracted are from food materials, and participation bacterium of fermenting is even more people The component part of body microecosystem.Therefore, the side effect of ferment is almost nil, powerful effect, minimum side effect, in recent years Come always in explosive increase trend.
Jerusalem artichoke is a kind of asteraceae helianthus perennial root herbaceous plant, edible, cook or cook gruel, salted white, solarization System.The existing further exploitation of Jerusalem artichoke has Jerusalem artichoke beverage (CN102258209A), Jerusalem artichoke wine (CN104531431A), but these are produced Product are not enough the extraction of nutriment ingredient in raw material, and application range is smaller.
Summary of the invention
In view of this, solving biography the purpose of the present invention is to provide a kind of Jerusalem artichoke probiotics ferment and preparation method thereof The narrow problem of system Jerusalem artichoke application approach.
The technical scheme adopted by the invention is as follows:
A kind of preparation method of Jerusalem artichoke probiotics ferment, includes the following steps:
S1 is by Jerusalem artichoke 550-750KG, dragon fruit 50-100KG, hawthorn 100-150KG, Chinese yam 50-100KG and brown granulated sugar 50KG is added in Zymolysis Equipment;
S2 continuously adds saccharomycete 5-10g and lactobacillus fermenti 5-10g into Zymolysis Equipment and carries out just fermentation, fermentation time It is 5-7 months;Just after fermentation be added lactobacillus paracasei 1-5g, lactobacillus plantarum 1-5g, Lactobacillus rhamnosus 1-5g into Second of fermentation of row, fermentation time 7-9 months;For the second time after fermentation be added lactobacillus acidophilus 5-10g, acetic acid bacteria 1-5g after Continuous third time fermentation 3-5 months;
After tri- stage fermentation of S3, detection pH stablizes between 2.4-3.6, and when alcoholic strength < 0.5%, fermentation is completed;
The fermentation liquid that fermentation is completed is filtered by S4;
S5 stands purifying:By filtered fermentation liquid stand purify, when alcoholic strength < 0.5%, lactic acid bacteria sum >=1 × 106It purifies and completes when CFU/ml, obtain Jerusalem artichoke probiotics enzyme stoste.
In the S2 when just fermentation, periodic agitation, progress aerobic fermentation, second of fermentation and third time are sent out in fermentation process When ferment, isolation air carries out anaerobic fermentation.
Zymolysis Equipment is fermenter.
Raw material is added before Zymolysis Equipment in S1, first passes through cleaning, drying, disinfection, crushing.
Using Jerusalem artichoke probiotics ferment made from method made above.
The progress of Jerusalem artichoke probiotics enzyme stoste is filling to obtain finished product.
Filling condition:Control Cleanliness reaches 100,000 grades of prescribed requirements, shop worker now washes your hands before entering workshop, It changes one's clothes, sterilize, strictly operated by the operating instruction of bottle placer and Cover whirling Machine, the filling amount check at any time in filling progress, The links such as seal quality, it is ensured that product complies with standard technical requirements.Before filling the container afterwards by equipment and pipeline carry out thoroughly cleaning and Disinfection;Filling is bottled, bottle and bottle cap cleaning:Bottle first uses pure water reverse-flush, then is dried up with clean compressed air, lid It is carried out disinfection processing with disinfection cabinet, guarantee reaches sterility requirements.
It is preferred that following technical scheme:In S2, fermenting bucket is 850, and each 1200 liters of fermentation ladle capacity (adds every time Enter raw material total amount 1000kg or so), electronic scale 2,1 ton of every fermenter charge, fermentation temperature is room temperature, and workshop sealing is protected Cleaning, normal temperature fermentation are held, fermentation time is 24 months.
In S5, standing purifying tank is 50, and it is food grade plastic using material that each standing purifying tankage size, which is 30 tons, Material.
When filling, the model TD-8 of bottle placer, the quantity of bottle placer is 2 sets;The model TD-SP1 of Cover whirling Machine, Cover whirling Machine Quantity be 2 sets, filling speed be 2000 bottles/hour, bottle device be 30ml vial.
When bottle and bottle cap clean, bottle device 2 are managed;Full-automatic inverting bottle cleaning machine 2, the model of full-automatic inverting bottle cleaning machine CP-15;Disinfection cabinet 1, the model ZTP-390 of disinfection cabinet.
Compared with prior art, the method have the benefit that:
The present invention using Jerusalem artichoke, dragon fruit, hawthorn, Chinese yam as primary raw material, take in scientific and reasonable by the nutritional ingredient of raw material It mixes more abundant, water is not added in preparation process, sufficiently ensured nutriment in ferment and activity substance content, dense Degree, under the fermentation of probiotics, while the type of strict control probiotics, dosage and fermentation duration, guarantee raw material Fermentation more sufficiently, depth, fermentation process is divided into three phases and is separately added into different strains according to the characteristic of different phase, Fermentation is set to have level more reasonable;Especially secondary fermentation stage, spontaneous fermentation are not further added by sugar source, finally make raw material In nutriment, active material, antioxidant can discharge to decomposite completely and, improve existing ferment using raw material Add ferment fermentation efficiency caused by the production method of water by fermentation or fruit juice fermentation low, active material, nutrient composition content are low, with And the technical problems such as health-care effect is not significant.It finally obtains a kind of good mouthfeel, Jerusalem artichoke probiotics ferment that can be hypoglycemic, increases Jerusalem artichoke probiotics ferment nutritive value.
Jerusalem artichoke probiotics ferment produced by the present invention is free of any food additives, safe and healthy, has no toxic side effect, can put The heart is enjoyed, and various people are suitable for;Solve that traditional Jerusalem artichoke application approach is narrow, product form Single-issue.
Specific embodiment
Illustrate a specific embodiment of the invention below with reference to embodiment, but following embodiment is used only to be described in detail The present invention does not limit the scope of the invention in any way.
Raw material is added before fermenter through over cleaning, drying, ultraviolet disinfection, crushing in following embodiment.
Embodiment 1:A kind of preparation method of Jerusalem artichoke probiotics ferment, includes the following steps:
Jerusalem artichoke 750KG, dragon fruit 50KG, hawthorn 100KG, Chinese yam 50KG and brown granulated sugar 50KG are added in fermenter S1;
S2 continuously adds saccharomycete 10g and lactobacillus fermenti 10g into fermenter and just ferment, in fermentation process daily Stirring carries out aerobic fermentation, and fermentation time is 6 months;Lactobacillus paracasei 5g, lactobacillus plantarum are just added after fermentation 5g, Lactobacillus rhamnosus 5g carry out second and ferment, and fermentation time 8 months;Lactobacillus acidophilus is added after fermentation for the second time 10g, acetic acid bacteria 5g continue third time fermentation 4 months;
When second of fermentation and third time are fermented, fermentation process fermenter overlay film sealing, isolation air carries out anaerobic fermentation;
After tri- stage fermentation of S3, detection pH stablizes between 2.4-3.6, and when alcoholic strength < 0.5%, fermentation is completed;
The fermentation liquid that fermentation is completed is filtered by S4;
S5 stands purifying:By filtered fermentation liquid stand purify, when alcoholic strength < 0.5%, lactic acid bacteria sum >=1 × 106It purifies and completes when CFU/ml, obtain Jerusalem artichoke probiotics enzyme stoste.
Embodiment 2:A kind of preparation method of Jerusalem artichoke probiotics ferment, includes the following steps:
Fermenter is added in Jerusalem artichoke 550KG, dragon fruit 100KG, hawthorn 150KG, Chinese yam 100KG and brown granulated sugar 50KG by S1 In;
S2 continuously adds saccharomycete 5g and lactobacillus fermenti 10g into fermenter and just ferment, in fermentation process daily Stirring carries out aerobic fermentation, and fermentation time is 5-7 months;Lactobacillus paracasei 1g, lactobacillus plantarum are just added after fermentation 5g, Lactobacillus rhamnosus 1g carry out second and ferment, and fermentation time 7-9 months;Acidophilus cream bar is added after fermentation for the second time Bacterium 5g, acetic acid bacteria 5g continue third time fermentation 3-5 months;
When second of fermentation and third time are fermented, fermentation process fermenter overlay film sealing, isolation air carries out anaerobic fermentation
After tri- stage fermentation of S3, detection pH stablizes between 2.4-3.6, and when alcoholic strength < 0.5%, fermentation is completed;
The fermentation liquid that fermentation is completed is filtered by S4;
S5 stands purifying:By filtered fermentation liquid stand purify, when alcoholic strength < 0.5%, lactic acid bacteria sum >=1 × 106It purifies and completes when CFU/ml, obtain Jerusalem artichoke probiotics enzyme stoste.
Embodiment 3:A kind of preparation method of Jerusalem artichoke probiotics ferment, includes the following steps:
Jerusalem artichoke 600KG, dragon fruit 80KG, hawthorn 120KG, Chinese yam 80KG and brown granulated sugar 50KG are added in fermenter S1;
S2 continuously adds saccharomycete 8g and lactobacillus fermenti 8g into fermenter and carries out just fermentation, stirs daily in fermentation process It mixes, carry out aerobic fermentation, fermentation time is 6 months;Just after fermentation be added lactobacillus paracasei 3g, lactobacillus plantarum 3g, Lactobacillus rhamnosus 3g carries out second and ferments, and fermentation time 8 months;For the second time after fermentation be added lactobacillus acidophilus 8g, Acetic acid bacteria 3g continues third time fermentation 4 months;
When second of fermentation and third time are fermented, fermentation process fermenter overlay film sealing, isolation air carries out anaerobic fermentation
After tri- stage fermentation of S3, detection pH stablizes between 2.4-3.6, and when alcoholic strength < 0.5%, fermentation is completed;
The fermentation liquid that fermentation is completed is filtered by S4;
S5 stands purifying:By filtered fermentation liquid stand purify, when alcoholic strength < 0.5%, lactic acid bacteria sum >=1 × 106It purifies and completes when CFU/ml, obtain Jerusalem artichoke probiotics enzyme stoste.
Embodiment 4:A kind of preparation method of Jerusalem artichoke probiotics ferment, includes the following steps:
Fermenter is added in Jerusalem artichoke 700KG, dragon fruit 100KG, hawthorn 100KG, Chinese yam 100KG and brown granulated sugar 50KG by S1 In;
S2 continuously adds saccharomycete 10g and lactobacillus fermenti 5g into fermenter and just ferment, in fermentation process daily Stirring carries out aerobic fermentation, and fermentation time is 5-7 months;Lactobacillus paracasei 5g, lactobacillus plantarum are just added after fermentation 1g, Lactobacillus rhamnosus 5g carry out second and ferment, and fermentation time 7-9 months;Acidophilus cream bar is added after fermentation for the second time Bacterium 10g, acetic acid bacteria 1g continue third time fermentation 3-5 months;
When second of fermentation and third time are fermented, fermentation process fermenter overlay film sealing, isolation air carries out anaerobic fermentation
After tri- stage fermentation of S3, detection pH stablizes between 2.4-3.6, and when alcoholic strength < 0.5%, fermentation is completed;
The fermentation liquid that fermentation is completed is filtered by S4;
S5 stands purifying:By filtered fermentation liquid stand purify, when alcoholic strength < 0.5%, lactic acid bacteria sum >=1 × 106It purifies and completes when CFU/ml, obtain Jerusalem artichoke probiotics enzyme stoste.
After embodiment 1-4 obtains Jerusalem artichoke probiotics enzyme stoste, microbiological indicator, again need to be detected by national standard GB/T31121 Metal indicator is up to standard, other indexs reach professional standard T/CBFIA08003-2016, and then Jerusalem artichoke probiotics enzyme stoste is through special It is piped to racking room, it is filling to obtain Jerusalem artichoke probiotics ferment finished product.
Effect experiment:
This embodiment is the hyperglycemia model using big white mouse, enumerates by the preparation of 1-3 method of the embodiment of the present invention The blood sugar decreasing effect of Jerusalem artichoke probiotics ferment.
Experiment content:The Jerusalem artichoke probiotics ferment prepared in Example 1-3 is as test sample, using FORA Fu Er blood Sugar determination instrument measures blood glucose value.Jerusalem artichoke probiotics ferment mean intake is calculated according to using the average magnitude of discharge reduction.
Experiment screening weight keeps being fitted under rearing conditions same case at male white rat 160 of 185-195g Answering property is raised three days, is then grouped into 4 groups at random;The Jerusalem artichoke probiotics ferment prepared in 1 feeding embodiment 1 of experimental group;Experiment Organize Jerusalem artichoke probiotics ferment after diluting in 2 feeding embodiments 2, the Jerusalem artichoke probiotics ferment prepared in 3 feeding embodiment 3 of experimental group Element;Blank group big white mouse feeding normal drinking water.To the continuous feeding of four groups of big white mouse 30 days, and periodic measurement blood glucose value was compared Compared with specific experiment result see the table below.
Influence of the Jerusalem artichoke probiotics ferment to big white mouse blood glucose
Before feeding:Blank group, experimental group 1, experimental group 2, blood glucose value verifies modeling without apparent difference between experimental group 3 Success;Feeding 15 days, 30 days, experimental group 1, experimental group 2, experimental group 3 and blank group comparison big white mouse blood glucose value had significant drop It is low, illustrate that the Jerusalem artichoke probiotics ferment of embodiment 1-3 preparation has effect of lowering blood sugar, and Jerusalem artichoke ferment obtained by embodiment 1 Blood sugar decreasing effect is best.
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, this field is common Other modifications or equivalent replacement that technical staff makes technical solution of the present invention, without departing from technical solution of the present invention Spirit and scope, be intended to be within the scope of the claims of the invention.

Claims (5)

1. a kind of preparation method of Jerusalem artichoke probiotics ferment, which is characterized in that include the following steps:
S1 is by Jerusalem artichoke 550-750KG, dragon fruit 50-100KG, 50 KG of hawthorn 100-150KG, Chinese yam 50-100KG and brown granulated sugar It is added in Zymolysis Equipment;
S2 continuously adds saccharomycete 5-10g and lactobacillus fermenti 5-10g into Zymolysis Equipment and carries out just fermentation, fermentation time 5- 7 months;Lactobacillus paracasei 1-5g, lactobacillus plantarum 1-5g, Lactobacillus rhamnosus 1-5g are just added after fermentation and carries out the Secondary fermentation, fermentation time 7-9 months;Lactobacillus acidophilus 5-10g, acetic acid bacteria 1-5g is added after fermentation for the second time and continues the Fermentation 3-5 months three times;
After tri- stage fermentation of S3, detection pH stablizes between 2.4-3.6, and when alcoholic strength < 0.5%, fermentation is completed;
The fermentation liquid that fermentation is completed is filtered by S4;
S5 stands purifying:Filtered fermentation liquid is stood and is purified, as alcoholic strength < 0.5%, lactic acid bacteria sum >=1 × 106CFU/ It purifies and completes when ml, obtain Jerusalem artichoke probiotics enzyme stoste.
2. the preparation method of Jerusalem artichoke probiotics ferment according to claim 1, it is characterised in that:In the S2 when just fermentation, When periodic agitation, progress aerobic fermentation, second of fermentation and third time are fermented in fermentation process, completely cuts off air and carry out anaerobism hair Ferment.
3. the preparation method of Jerusalem artichoke probiotics ferment according to claim 1, it is characterised in that:Zymolysis Equipment is fermentation Bucket.
4. the preparation method of Jerusalem artichoke probiotics ferment according to claim 1, it is characterised in that:Fermentation is added in raw material in S1 Before equipment, cleaning, drying, disinfection, crushing are first passed through.
5. using Jerusalem artichoke probiotics ferment made from any one of claim 1-4 preparation method.
CN201810806059.8A 2018-07-20 2018-07-20 A kind of Jerusalem artichoke probiotics ferment and preparation method thereof Pending CN108902947A (en)

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103039883A (en) * 2013-01-16 2013-04-17 周礼红 Yangtao lucid ganoderma composite ferment and preparation method thereof
CN106578794A (en) * 2016-12-01 2017-04-26 李恒 Fermented fruit and vegetable juice beverage preparation process
CN106942742A (en) * 2017-03-30 2017-07-14 四川久润泰科技有限公司 A kind of preparation method of fig ferment
CN107019213A (en) * 2017-04-21 2017-08-08 湖南双晟科技信息咨询有限公司 A kind of water chestnut ferment and preparation method thereof
CN107348272A (en) * 2017-06-01 2017-11-17 扬生(南召)生物科技有限公司 A kind of probiotics fruits and vegetables enzyme beverage and preparation method thereof
CN107874249A (en) * 2017-12-06 2018-04-06 青海丹噶尔农业产业化有限责任公司 A kind of manufacture method of pectase
CN107981342A (en) * 2017-12-12 2018-05-04 贵州德龙食品科技有限公司 A kind of production technology of dragon fruit ferment in good taste

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103039883A (en) * 2013-01-16 2013-04-17 周礼红 Yangtao lucid ganoderma composite ferment and preparation method thereof
CN106578794A (en) * 2016-12-01 2017-04-26 李恒 Fermented fruit and vegetable juice beverage preparation process
CN106942742A (en) * 2017-03-30 2017-07-14 四川久润泰科技有限公司 A kind of preparation method of fig ferment
CN107019213A (en) * 2017-04-21 2017-08-08 湖南双晟科技信息咨询有限公司 A kind of water chestnut ferment and preparation method thereof
CN107348272A (en) * 2017-06-01 2017-11-17 扬生(南召)生物科技有限公司 A kind of probiotics fruits and vegetables enzyme beverage and preparation method thereof
CN107874249A (en) * 2017-12-06 2018-04-06 青海丹噶尔农业产业化有限责任公司 A kind of manufacture method of pectase
CN107981342A (en) * 2017-12-12 2018-05-04 贵州德龙食品科技有限公司 A kind of production technology of dragon fruit ferment in good taste

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Address after: 474650 Taiwan Huayang Longsheng Science and Technology Industrial Park, Nanzhao County, Nanyang City, Henan Province

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Application publication date: 20181130