CN108902630A - A kind of prevacuum autoclaving method for sterilization of liquids - Google Patents

Abstract

The present invention provides a kind of prevacuum autoclaving methods for sterilization of liquids, pass sequentially through forevacuum, heating, sterilizing, steps of exhausting, make in closed container steam be rapidly achieved saturation state quickly to heat liquid, it sterilizes after fluid temperature reaches scheduled temperature, it is sufficiently heated to make to be sterilized article, achievees the purpose that sterilizing.The present invention enables temperature fully to penetrate into the container equipped with liquid in 110~120 DEG C of water vapour by way of making vacuumize process before heating, has the function that quickly to kill microorganism.Since the pressure of this method is no more than 100kPa, it is not belonging to the scope of pressure vessel, greatly improves the safety used.

Description

A pre-vacuum pressure steam sterilization method for liquid sterilization

technical field

The invention relates to the field of liquid sterilization, in particular to a pre-vacuum pressure steam sterilization method for liquid sterilization suitable for agricultural tissue culture or food industry.

Background technique

High-pressure steam sterilization technology has the advantages of simple operation, reliable sterilization effect, and no need to add additional disinfectants. In addition to the pharmaceutical industry, it is also widely used in agricultural tissue culture, food processing and production, and biotechnology research.

For containers with a working pressure greater than 100kPa (120°C) and a volume of more than 30 liters, they are special equipment pressure vessels. From the design, manufacture and use, the user must follow the requirements of TSG21-2016 "Safety Technical Supervision Regulations for Stationary Pressure Vessels". Most of the pressure steam sterilizers currently on the market use a sterilization temperature of 121°C or above, and a working pressure of more than 100kPa, which needs to be produced and used in accordance with the regulations of TSG 21-2016.

At present, liquid sterilization generally adopts the high-pressure steam down-discharge type 121°C (110kPa), 20 minutes sterilization specification. The principle of sterilization is: put high-temperature and high-pressure water vapor into a closed container, use the characteristic that cold air is heavier than steam, press the cold air on the lower part of the container, open the valve at the bottom of the container, and the pressure will discharge the cold air downwards ( This process is called decooling), then close the valve, and then re-introduce high-temperature and high-pressure steam. At this time, the saturated steam heats up the liquid in the container (the liquid is in a bottle or container), and the bottle When the liquid inside boils, the cold air remaining in the bottle can be discharged upwards out of the bottle. When the temperature reaches the predetermined sterilization temperature, the sterilization timer will be carried out. After the timing is completed, the steam in the container will be discharged, and the sterilization is completed. The temperature (pressure) and time curve of the sterilization process is shown in Figure 1. The high temperature during the sterilization process may cause the active components of the solution to decompose. If the sterilization temperature can be lowered, it is more conducive to the preservation of the active components of the solution.

Contents of the invention

For the above problems, the invention provides a kind of pre-vacuum pressure steam sterilization method for liquid sterilization, the technical scheme is as follows:

A pre-vacuum pressure steam sterilization method for liquid sterilization, through the steps of pre-vacuum, temperature rise, sterilization, and exhaust in sequence, so that the steam in the closed container can quickly reach a saturated state and quickly heat the liquid. When the temperature of the liquid reaches a predetermined Sterilize after the temperature is high, so that the sterilized items are fully heated to achieve the purpose of sterilization.

Preferably, in the pre-vacuum step, the pressure in the inner chamber of the airtight container is -50 ~ -90kPa.

Preferably, in the pre-vacuum step, the air in the bottle or vessel containing the liquid is sucked out.

Preferably, the temperature raising step is to feed high-temperature and high-pressure steam to make the liquid temperature reach 110-120° C., and the pressure in the inner chamber of the airtight container is 50-100 kPa.

Preferably, the sterilization time in the sterilization step is 15-40 minutes.

The pre-vacuum pressure steam sterilization method for liquid sterilization provided by the present invention is to make the water vapor at a temperature of 110-120°C fully penetrate into the container containing the liquid by vacuumizing before the temperature rises to achieve Quickly kills microorganisms. Because the pressure of the method does not exceed 100kPa, it does not belong to the category of pressure vessels, greatly improving the safety of use.

Advantage 1: The use of pre-vacuum sterilization is a change in the sterilization of the traditional tissue culture industry, which can achieve rapid, energy-saving, environmentally friendly, and reliable sterilization.

Advantage 2: The pressure is low, which reduces the requirements for the selection of raw materials, the selection of components, and the structural design, and accelerates industrialization on the premise of ensuring the sterilization effect, saving energy and reducing consumption, and reducing costs.

Advantage 3: By lowering the sterilization temperature, the nutrient content of the medium can be effectively protected and the tissue culture efficiency can be increased.

Advantage 4: Because the sterilization temperature is lowered, the heating time is reduced, which can save a lot of electric energy and reduce heat emission.

Advantage 5: Since it does not belong to the supervision of pressure vessels, the filing process can be simplified, and the operator does not need special equipment for filing and registration.

Description of drawings

The accompanying drawings are used to provide a further understanding of the present invention, and constitute a part of the description, and are used together with the embodiments of the present invention to explain the present invention, and do not constitute a limitation to the present invention. In the attached picture:

Fig. 1 is the curve diagram of temperature (pressure) and time in the existing down-row type sterilization process.

Fig. 2 is a graph of temperature (pressure) and time for pre-vacuum sterilization according to the embodiment of the present invention.

Detailed ways

The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

The invention relates to the technical field of liquid sterilization, in particular to the pressure steam sterilization method for culture liquid, solution and the like.

As shown in Figure 2, it is the temperature (pressure) and time curve diagram of the pre-vacuum sterilization process of the embodiment of the present invention. In the pre-vacuum process of the airtight container, the inner chamber pressure is pumped to -50～-90kPa, and the liquid can be housed simultaneously. The air in the bottle or container is pumped out, and then high-temperature and high-pressure steam is introduced. Due to the vacuum state, the steam quickly reaches saturation and heats the liquid rapidly. When the liquid temperature reaches the predetermined 110-120°C (50-100kPa), the sterilization timing is carried out 15 to 40 minutes, so that the sterilized items are fully heated to achieve the purpose of sterilization.

Although the embodiments of the present invention have been shown and described, those skilled in the art can understand that various changes, modifications and substitutions can be made to these embodiments without departing from the principle and spirit of the present invention. and modifications, the scope of the invention is defined by the appended claims and their equivalents.

Claims (5)

1. a kind of prevacuum autoclaving method for sterilization of liquids, it is characterised in that：

Forevacuum, heating, sterilizing, steps of exhausting are passed sequentially through, steam in closed container is made to be rapidly achieved saturation state to liquid Quickly heating, sterilizes after fluid temperature reaches scheduled temperature, and it is sufficiently heated to make to be sterilized article, reaches the mesh of sterilizing 's.

2. a kind of prevacuum autoclaving method for sterilization of liquids as described in claim 1, it is characterised in that：

It is -50~-90kPa by chamber pressure in closed container in the forevacuum step.

3. a kind of prevacuum autoclaving method for sterilization of liquids as claimed in claim 2, it is characterised in that：

In the forevacuum step, the air in bottle or vessel equipped with liquid is extracted out.

4. a kind of prevacuum autoclaving method for sterilization of liquids as described in claim 1, it is characterised in that：

The heating step is to be passed through high temperature and high pressure steam, so that fluid temperature is reached 110~120 DEG C, chamber pressure in closed container 50~100kPa.

5. a kind of prevacuum autoclaving method for sterilization of liquids as described in claim 1, it is characterised in that：

Sterilization time in the sterilization steps is 15~40 minutes.