CN108902630A - A kind of prevacuum autoclaving method for sterilization of liquids - Google Patents
A kind of prevacuum autoclaving method for sterilization of liquids Download PDFInfo
- Publication number
- CN108902630A CN108902630A CN201810908499.4A CN201810908499A CN108902630A CN 108902630 A CN108902630 A CN 108902630A CN 201810908499 A CN201810908499 A CN 201810908499A CN 108902630 A CN108902630 A CN 108902630A
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- China
- Prior art keywords
- sterilization
- liquids
- sterilizing
- forevacuum
- pressure
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/015—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with pressure variation, shock, acceleration or shear stress or cavitation
- A23L3/0155—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with pressure variation, shock, acceleration or shear stress or cavitation using sub- or super-atmospheric pressures, or pressure variations transmitted by a liquid or gas
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/04—Heat
- A61L2/06—Hot gas
- A61L2/07—Steam
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
Abstract
The present invention provides a kind of prevacuum autoclaving methods for sterilization of liquids, pass sequentially through forevacuum, heating, sterilizing, steps of exhausting, make in closed container steam be rapidly achieved saturation state quickly to heat liquid, it sterilizes after fluid temperature reaches scheduled temperature, it is sufficiently heated to make to be sterilized article, achievees the purpose that sterilizing.The present invention enables temperature fully to penetrate into the container equipped with liquid in 110~120 DEG C of water vapour by way of making vacuumize process before heating, has the function that quickly to kill microorganism.Since the pressure of this method is no more than 100kPa, it is not belonging to the scope of pressure vessel, greatly improves the safety used.
Description
Technical field
The present invention relates to sterilization of liquids fields, more particularly to are suitable for agriculture one of tissue culture or food industry and are used for liquid
The prevacuum autoclaving method of body sterilizing.
Background technique
High pressure steam sterilization technology has the advantages that easy to operate, sterilization effect is reliable, does not need to add additional disinfectant,
Other than pharmaceuticals industry, also have a wide range of applications in fields such as agriculture tissue culture, food processing and production, biotechnology research.
It is greater than the container of 30 liters of volume of 100kPa (120 DEG C) or more for operating pressure, is to belong to special equipment pressure appearance
Device.From design, manufacture and use all users must be according to TSG21-2016《Fixed safe technology of pressure vessel Supervision Code》
Requirement.Pressure steam sterilizer majority on the market uses 121 DEG C or more of sterilising temp at present, and operating pressure is more than
100kPa needs to carry out by the regulation of TSG 21-2016 to produce and using operation.
Sterilization of liquids generallys use 121 DEG C (110kPa) of high steam lower-discharging type, the specification of sterilizing in 20 minutes at present.It goes out
Bacterium principle is：It is passed through high-temperature high-pressure steam in closed container, the feature using cold air than steam weight, by cold air pressure
In the lower part of container, the valve of container bottom is opened, cold air is discharged downwards (this process is known as arranging cold), turned off by pressure
Valve, is then passed through the steam of high temperature and pressure again, reaches the steam of saturation state at this time, to the liquid (liquid being placed in container
Body is in bottle or vessel) in bottle when liquid boiling bottle can be discharged in the cold air remained in bottle by heat temperature raising upwards
Outside, when temperature reaches predetermined sterilising temp, when carrying out sterilometer, the steam in container is discharged after timing, has been sterilized
At.Sterilization process temperature (pressure), time plot are shown in that Fig. 1, the high temperature of sterilization process may result in the effective component of solution
It decomposes, if the preservation that sterilising temp is more advantageous to solution effective component can be reduced.
Summary of the invention
In view of the above problems, the present invention provides a kind of prevacuum autoclaving method for sterilization of liquids, skill
Art scheme is as follows：
A kind of prevacuum autoclaving method for sterilization of liquids, passes sequentially through forevacuum, heating, sterilizing, row
Jia bombardier is rapid, so that steam is rapidly achieved saturation state and quickly heats to liquid, when fluid temperature reaches scheduled temperature
It sterilizes after degree, it is sufficiently heated to make to be sterilized article, achievees the purpose that sterilizing.
It preferably, is -50~-90kPa by chamber pressure in closed container in the forevacuum step.
Preferably, in the forevacuum step, the air in bottle or vessel equipped with liquid is extracted out.
Preferably, the heating step is to be passed through high temperature and high pressure steam, so that fluid temperature is reached 110~120 DEG C, closed appearance
50~100kPa of chamber pressure in device.
Preferably, the sterilization time in the sterilization steps is 15~40 minutes.
Provided by the present invention for sterilization of liquids prevacuum autoclaving method by being vacuumized before heating
The mode of processing enables temperature fully to penetrate into the container equipped with liquid in 110~120 DEG C of water vapour, reaches quick
Kill the effect of microorganism.Since the pressure of this method is no more than 100kPa, it is not belonging to the scope of pressure vessel, greatly improving makes
Safety.
Advantage 1：It is sterilized using forevacuum formula, is that the sterilizing to traditional tissue culture industry is changed, can reach quick, energy saving, ring
It protects, and it is reliable to sterilize.
Advantage 2：Pressure is lower, reduces selection to raw material, to the type selecting of component, require structure design etc.,
Accelerate industrialization under the premise of guaranteeing sterilization effect, it is energy-saving, reduce cost.
Advantage 3：By reducing sterilising temp, it can be effectively protected the nutritional ingredient of culture medium, increase tissue culture efficiency.
Advantage 4：Because sterilising temp reduces, the heating-up time is reduced, a large amount of electric energy can be saved, heat is reduced and distributes.
Advantage 5：Due to being not belonging to pressure vessel supervision, process of putting on record can simplify, operator puts on record without special equipment
Registration.
Detailed description of the invention
Attached drawing is used to provide further understanding of the present invention, and constitutes part of specification, with reality of the invention
It applies example to be used to explain the present invention together, not be construed as limiting the invention.In the accompanying drawings：
Fig. 1 is existing lower-discharging type sterilization process temperature (pressure), time plot.
Fig. 2 is using forevacuum of embodiment of the present invention sterilising temp (pressure), time plot.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
The present invention relates to sterilization of liquids technical fields, especially the pressuresteam sterilization mode for culture solution, solution etc..
It is illustrated in figure 2 forevacuum sterilization process temperature of the embodiment of the present invention (pressure), time plot, in closed container
Interior chamber pressure is evacuated to -50~-90kPa, while the air in bottle or vessel equipped with liquid can taken out by forevacuum process
Out, then it is passed through high temperature and high pressure steam, due to vacuum state, steam is rapidly achieved saturation state and quickly heats to liquid, works as liquid
It after temperature reaches scheduled 110~120 DEG C (50~100kPa), carries out when sterilometer 15~40 minutes, makes to be sterilized article and fill
Divide and be heated, achievees the purpose that sterilizing.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (5)
1. a kind of prevacuum autoclaving method for sterilization of liquids, it is characterised in that：
Forevacuum, heating, sterilizing, steps of exhausting are passed sequentially through, steam in closed container is made to be rapidly achieved saturation state to liquid
Quickly heating, sterilizes after fluid temperature reaches scheduled temperature, and it is sufficiently heated to make to be sterilized article, reaches the mesh of sterilizing
's.
2. a kind of prevacuum autoclaving method for sterilization of liquids as described in claim 1, it is characterised in that：
It is -50~-90kPa by chamber pressure in closed container in the forevacuum step.
3. a kind of prevacuum autoclaving method for sterilization of liquids as claimed in claim 2, it is characterised in that：
In the forevacuum step, the air in bottle or vessel equipped with liquid is extracted out.
4. a kind of prevacuum autoclaving method for sterilization of liquids as described in claim 1, it is characterised in that：
The heating step is to be passed through high temperature and high pressure steam, so that fluid temperature is reached 110~120 DEG C, chamber pressure in closed container
50~100kPa.
5. a kind of prevacuum autoclaving method for sterilization of liquids as described in claim 1, it is characterised in that：
Sterilization time in the sterilization steps is 15~40 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810908499.4A CN108902630A (en) | 2018-08-10 | 2018-08-10 | A kind of prevacuum autoclaving method for sterilization of liquids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810908499.4A CN108902630A (en) | 2018-08-10 | 2018-08-10 | A kind of prevacuum autoclaving method for sterilization of liquids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108902630A true CN108902630A (en) | 2018-11-30 |
Family
ID=64404984
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810908499.4A Withdrawn CN108902630A (en) | 2018-08-10 | 2018-08-10 | A kind of prevacuum autoclaving method for sterilization of liquids |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108902630A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112790325A (en) * | 2021-02-01 | 2021-05-14 | 广东省农业科学院蚕业与农产品加工研究所 | Nutrition-enhanced stabilized rice bran rapid processing method |
| CN113876969A (en) * | 2021-08-16 | 2022-01-04 | 广州市豪尔生医疗设备有限公司 | Pre-vacuum sub-high pressure steam sterilization process for liquid culture medium |
| US11395860B2 (en) | 2019-03-05 | 2022-07-26 | Grifols Worldwide Operations Limited | Method of preparing containers for blood-derived products |
-
2018
- 2018-08-10 CN CN201810908499.4A patent/CN108902630A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11395860B2 (en) | 2019-03-05 | 2022-07-26 | Grifols Worldwide Operations Limited | Method of preparing containers for blood-derived products |
| CN112790325A (en) * | 2021-02-01 | 2021-05-14 | 广东省农业科学院蚕业与农产品加工研究所 | Nutrition-enhanced stabilized rice bran rapid processing method |
| CN113876969A (en) * | 2021-08-16 | 2022-01-04 | 广州市豪尔生医疗设备有限公司 | Pre-vacuum sub-high pressure steam sterilization process for liquid culture medium |
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| PB01 | Publication | ||
| PB01 | Publication | ||
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| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20181130 |