CN108893407A - The flow-compensated system and method for plankton sampler - Google Patents
The flow-compensated system and method for plankton sampler Download PDFInfo
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- CN108893407A CN108893407A CN201810684001.0A CN201810684001A CN108893407A CN 108893407 A CN108893407 A CN 108893407A CN 201810684001 A CN201810684001 A CN 201810684001A CN 108893407 A CN108893407 A CN 108893407A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
Abstract
The invention discloses the flow-compensated system and methods of plankton sampler, wherein method includes the following steps：After obtaining preset vol value, blower work time parameter is obtained according to preset vol value；It is worked according to working time state modulator blower, and calculates the flow volume of acquisition according to flow parameter after the flow parameter of the air on acquisition air flue in real time；Blower compensating parameter is obtained according to flow volume and preset vol value；The working condition that blower is controlled according to blower compensating parameter, until blower stops.The present invention calculates flow volume by flow parameter in real time, and after combining flow volume preset vol value to obtain blower compensating parameter, according to blower compensating parameter volumetric errors, to improve the accuracy of acquisition microorganism, air microbe concentration can be truly reflected, the requirement for meeting high accuracy can be widely applied to plankton sampling field.
Description
Technical field
The present invention relates to plankton sampling field more particularly to a kind of flow-compensated systems of plankton sampler
System and method.
Background technique
Plankton sampling, is to be collected the microbial aerosol of sparse distribution in air by certain method
It in the surface of limitation and the medium of small size, is analyzed in next step to carry out, such product is usually used in the inspection to air microbe
In survey.Existing plankton sample devices is not monitored in sampling to by the flow total volume of air flue, and only
It is certain section of time flow of measurement in conjunction with time calculating flow total volume, the flow that actually such sample mode can acquire is overall
It is long-pending totally to contain error with preset flow, so collected microbiological data also has error, it is unfavorable for air microbe
The true reflection of concentration, is unable to satisfy the requirement of high accuracy.
Summary of the invention
In order to solve the above-mentioned technical problem, the object of the present invention is to provide a kind of compensation systems for eliminating acquisition flow error
System.
It is a further object of the present invention to provide a kind of compensation methodes for eliminating acquisition flow error.
The technical scheme adopted by the invention is that：
The flow-compensated system of plankton sampler, including control main module, microprocessor, communication module, blower
With the air flow sensor being arranged on air flue, the air flow sensor is connect with microprocessor, the microprocessor
It is connect by communication module with control main module, the control main module is connect with blower；
The air flow sensor sends flow parameter for acquiring the flow parameter of the air on air flue in real time
To microprocessor；
After the microprocessor is used to obtain blower work time parameter according to preset vol value, the blower working time is joined
Number is sent to control main module, and after the flow volume according to flow parameter calculating acquisition, according to flow volume and default body
Product value obtains blower compensating parameter, and blower compensating parameter is sent to control main module；
The control main module is used for the work shape according to blower work time parameter and blower compensating parameter control blower
State.
Further, the working condition of the blower is divided into three phases, and the three phases, which are followed successively by, accelerates starting period, even
Fast phase and even deceleration withholding period.
Further, the blower work time parameter includes blower work in the first time period and blower for accelerating the starting period
The second time period to work in the at the uniform velocity phase, described the step of blower work time parameter is obtained according to preset vol value, specially：
According to preset vol value and preset blower running parameter, calculates separately blower work and accelerating the first of the starting period
The second time period of period and work in the at the uniform velocity phase；
The preset blower running parameter includes blower work in the predetermined acceleration and blower work for accelerating the starting period
In the preset flow value of at the uniform velocity phase.
Further, the blower compensating parameter includes third period and acceleration of the blower work in even withholding period of slowing down
Degree, includes the following steps described the step of obtaining blower compensating parameter according to flow volume and preset vol value：
Flow volume is calculated according to flow parameter in real time, and when blower worked to even deceleration withholding period, in conjunction with flow body
Long-pending and preset vol value obtains compensation bulking value；
According to compensation bulking value and preset flow value obtain blower work it is even slow down withholding period the third period and plus
Speed.
Further, the blower compensating parameter further includes power adjustment parameters, it is described according to blower work time parameter and
Blower compensating parameter controls the step of working condition of blower, specially：
In first time period, blower is controlled according to predetermined acceleration and is accelerated in first time period；
In second time period, the operating power of blower is adjusted in real time according to power adjustment parameters, make the flow ginseng of acquisition
Number is equal to preset flow value；
Within the third period, uniformly accelerated motion is done according to Acceleration Control blower, until blower stops.
It further, further include control panel, the control panel is connect with control main module.
Another technical solution of the present invention is：
The flow-compensated method of plankton sampler, includes the following steps：
After obtaining preset vol value, blower work time parameter is obtained according to preset vol value；
It is worked according to working time state modulator blower, and in real time after the flow parameter of the air on acquisition air flue,
The flow volume of acquisition is calculated according to flow parameter；
Blower compensating parameter is obtained according to flow volume and preset vol value；
The working condition that blower is controlled according to blower compensating parameter, until blower stops.
Further, the working condition of the blower is divided into three phases, and the three phases are respectively to accelerate starting period, even
Fast phase and even deceleration withholding period.
Further, the blower work time parameter includes blower work in the first time period and blower for accelerating the starting period
The second time period to work in the at the uniform velocity phase, described the step of blower work time parameter is obtained according to preset vol value, specially：
According to preset vol value and preset blower running parameter, calculates separately blower work and accelerating the first of the starting period
The second time period of period and work in the at the uniform velocity phase；
The preset blower running parameter includes blower work in the predetermined acceleration and blower work for accelerating the starting period
In the preset flow value of at the uniform velocity phase.
Further, the blower compensating parameter includes third period and acceleration of the blower work in even withholding period of slowing down
Degree, includes the following steps described the step of obtaining blower compensating parameter according to flow volume and preset vol value：
Flow volume is calculated according to flow parameter in real time, and when blower worked to even deceleration withholding period, in conjunction with flow body
Long-pending and preset vol value obtains compensation bulking value；
According to compensation bulking value and preset flow value obtain blower work it is even slow down withholding period the third period and plus
Speed.
The beneficial effects of the invention are as follows：The present invention calculates flow volume by flow parameter in real time, and combines flow volume
After preset vol value obtains blower compensating parameter, according to blower compensating parameter volumetric errors, to improve the essence of acquisition microorganism
Exactness can be truly reflected air microbe concentration, meet the requirement of high accuracy.
Detailed description of the invention
Fig. 1 is the structural block diagram of the flow-compensated system of plankton sampler of the present invention；
Fig. 2 is the schematic diagram that air mass flow is acquired in three phases；
Fig. 3 is the step flow chart of the flow-compensated method of plankton sampler of the present invention.
Specific embodiment
Embodiment one
As shown in Figure 1, the flow-compensated system of plankton sampler, including control main module, microprocessor, communication
Module, blower and the air flow sensor being arranged on air flue, the air flow sensor is connect with microprocessor, described
Microprocessor is connect by communication module with control main module, and the control main module is connect with blower；
The air flow sensor sends flow parameter for acquiring the flow parameter of the air on air flue in real time
To microprocessor；
After the microprocessor is used to obtain blower work time parameter according to preset vol value, the blower working time is joined
Number is sent to control main module, and after the flow volume according to flow parameter calculating acquisition, according to flow volume and default body
Product value obtains blower compensating parameter, and blower compensating parameter is sent to control main module；
The control main module is used for the work shape according to blower work time parameter and blower compensating parameter control blower
State.
The working principle of above-mentioned compensation system is：The air flow sensor is arranged on air flue, for acquiring in real time
By the air mass flow of air flue.User pre-sets preset vol value, and the preset vol value is the air to be acquired
Bulking value, microprocessor calculate blower work time parameter according to preset vol value, control main module according to working time parameter
Control blower works, meanwhile, microprocessor acquires the flow parameter of the air on air flue in real time, and and is joined according to flow
After number calculates the flow volume of acquisition, blower compensating parameter is obtained according to flow volume and preset vol value, controls main mould root tuber
According to the working condition of blower compensating parameter control blower, until blower stops.Calculate the air of acquisition in real time by microprocessor
Flow volume, and combine preset vol to obtain blower compensating parameter, then control blower work by blower compensating parameter, to make
Collected flow volume improves the accuracy for improving acquisition microorganism, it is ensured that subsequent experiment as preset vol value
The accuracy of data；Since this compensation system can be truly reflected air microbe concentration, the requirement of high accuracy is met.
It is further used as preferred embodiment, the working condition of the blower is divided into three phases, the three phases
It is followed successively by and accelerates starting period, at the uniform velocity phase and even deceleration withholding period.
Blower is accelerating the starting period to accelerate, and blower moves with uniform velocity in the at the uniform velocity phase, and blower is in even deceleration withholding period
Uniformly accelerated motion is done, until blower stops.
It is further used as preferred embodiment, the blower work time parameter includes that blower work is accelerating the starting period
First time period and blower work second time period in the at the uniform velocity phase, it is described that the blower working time is obtained according to preset vol value
The step of parameter, specially：
According to preset vol value and preset blower running parameter, calculates separately blower work and accelerating the first of the starting period
The second time period of period and work in the at the uniform velocity phase；
The preset blower running parameter includes blower work in the predetermined acceleration and blower work for accelerating the starting period
In the preset flow value of at the uniform velocity phase.
In the at the uniform velocity phase, acquiring air mass flow by control blower is preset flow value, which is that user is preparatory
The value of setting, according to different environment, different demands, user can set different flow values.According to preset vol value and
Preset blower running parameter calculates first time period and second time period can pass through in the present embodiment there are many mode
Following manner is realized, can be calculated at the first time in conjunction with predetermined acceleration and the corresponding blower operating rate of preset flow value
Section；Preset vol value is obtained into quotient divided by preset flow value, then quotient is subtracted into first time period and obtains second time period.
It is further used as preferred embodiment, the blower compensating parameter includes blower work in even deceleration withholding period
Third period and acceleration, described the step of blower compensating parameter is obtained according to flow volume and preset vol value, including with
Lower step：
Flow volume is calculated according to flow parameter in real time, and when blower worked to even deceleration withholding period, in conjunction with flow body
Long-pending and preset vol value obtains compensation bulking value；
According to compensation bulking value and preset flow value obtain blower work it is even slow down withholding period the third period and plus
Speed.
It is further used as preferred embodiment, the blower compensating parameter further includes power adjustment parameters, the basis
The step of working condition of blower work time parameter and blower compensating parameter control blower, specially：
In first time period, blower is controlled according to predetermined acceleration and is accelerated in first time period；
In second time period, the operating power of blower is adjusted in real time according to power adjustment parameters, make the flow ginseng of acquisition
Number is equal to preset flow value；
Within the third period, uniformly accelerated motion is done according to Acceleration Control blower, until blower stops.
It is further used as preferred embodiment, further includes control panel, the control panel is connect with control main module.
User can input preset value, such as preset flow value and preset vol value etc. by control panel, facilitate user to system
Control operation.
In conjunction with Fig. 2, now above system is explained in detail.
The flow parameter of air on acquisition air flue in real time, the flow volume of acquisition is calculated according to flow parameter, is calculated public
Formula is as follows：Wherein QCollection capacityFor the flow volume of acquisition,To accelerate
The flow volume of starting period and at the uniform velocity phase acquisition will accelerate starting period and at the uniform velocity phase to be divided into multistage in time, calculates every section
Flow volume, wherein VnFor every section of average flow rate value, which is obtained by air flow sensor, TnFor each time
Section.As shown in Fig. 2, TaFor some period for accelerating the starting period, TbAnd TcFor two periods of at the uniform velocity phase.At the uniform velocity interim,
The sampling flow of air is preset flow value, the flow parameter of air is acquired in real time, when microprocessor determines the flow of acquisition
Parameter is not equal to preset flow value, then sends corresponding power adjustment parameters and give control main module, to make the flow of acquisition
Parameter is equal to preset flow value, such as when the flow parameter of acquisition is less than preset flow value, send adjust high-power information to
Main module is controlled, the power of blower is turned up according to information for control main module.This is because the gas in air is flowing, such as
When occurring strong wind in environment, the flow of acquisition can be indirectly influenced.In Fig. 2, (tl, t2) is first time period, and (t2, t3) is
Second time period, (t3, t4) are the third period.In the present embodiment, the value of (tl, t3) period is preset vol value and pre-
If the quotient of flow value, (tl, the t2) period can be obtained according to predetermined acceleration and preset flow value, obtain indirectly (t2,
T3) the period.When controlling blower and working to t3, compensation bulking value is obtained in conjunction with flow volume and preset vol value, further according to
It compensates bulking value and obtains third period (t3, t4) and acceleration of the blower work in even withholding period of slowing down.Wherein, Tn+1×Vn+1
To compensate bulking value, Tn+1For third period, Vn+1For the average flow rate of third period, in conjunction with compensation bulking value and stream is preset
Magnitude can obtain third period, Vn+1For the quotient for compensating bulking value and third period, V is being calculatedn+1Afterwards, wind can be calculated
Decel slope k=2 × V of the machine in the third periodn+1÷Tn+1, the k is acceleration of the blower work in even withholding period of slowing down
Degree, the acceleration value are adjusted according to the size of compensation bulking value, as shown in Fig. 2, when compensation bulking value is larger, (t3,
T4) increase, slope reduces, i.e., acceleration declines.By above-mentioned compensation, the flow volume of acquisition can be made to be equal to preset vol
Value improves the accuracy of acquisition microorganism, can be truly reflected air microbe concentration, meet the requirement of high accuracy.
In addition, above system, when acquiring plankton, using solid impact method, the air-flow of acquisition passes through narrow spray
Mouth forms high-speed flow directive culture medium, and after gas turns round along culture medium, aerosol is by inertial impaction and is adhered to culture
Base.The acquisition method compares other processing methods, and sampling volume is accurate, and particle-capture rate is high, light small and exquisite, is very suitable to move
Dynamic detection.
Above-mentioned compensation system is sampled applied to plankton, can meet following standard：1, GMP drug and food produces matter
Measure management regulation；2, the biological pollution control of the toilet ISO14698-1/2 and relevant control environment；3,GB/T 16293-2010
The test method of medical industry toilet (area) flcating germ.
Example two
As shown in figure 3, the flow-compensated method of plankton sampler, includes the following steps：
S1, after obtaining preset vol value, blower work time parameter is obtained according to preset vol value.
Wherein, the blower work time parameter includes blower work in first time period and the fanman for accelerating the starting period
Second time period of the work in the at the uniform velocity phase, described the step of blower work time parameter is obtained according to preset vol value, specially：Root
According to preset vol value and preset blower running parameter, blower work is calculated separately in the first time period and work for accelerating the starting period
Make the second time period in the at the uniform velocity phase.
S2, it is worked according to working time state modulator blower, and acquires the flow parameter of the air on air flue in real time
Afterwards, the flow volume of acquisition is calculated according to flow parameter.
S3, blower compensating parameter is obtained according to flow volume and preset vol value.
Wherein, the blower compensating parameter includes third period and acceleration of the blower work in even withholding period of slowing down,
Described the step of blower compensating parameter is obtained according to flow volume and preset vol value, including step S31~S32：
S31, flow volume calculated according to flow parameter in real time, and when blower worked to even deceleration withholding period, in conjunction with stream
It measures volume and preset vol value obtains compensation bulking value.
S32, blower work is obtained according to compensation bulking value and preset flow value in the third period of even withholding period of slowing down
And acceleration.
S4, the working condition that blower is controlled according to blower compensating parameter, until blower stops.The working condition of the blower
It is divided into three phases, the three phases are respectively to accelerate starting period, at the uniform velocity phase and even deceleration withholding period.
The above method calculates flow volume by flow parameter in real time, and flow volume preset vol value is combined to obtain wind
After machine compensating parameter, according to blower compensating parameter volumetric errors, so that the accuracy of acquisition microorganism is improved, it can be truly anti-
Air microbe concentration is reflected, the requirement of high accuracy is met.
In addition, the above method, when acquiring plankton, using solid impact method, the air-flow of acquisition passes through narrow spray
Mouth forms high-speed flow directive culture medium, and after gas turns round along culture medium, aerosol is by inertial impaction and is adhered to culture
Base.The acquisition method compares other processing methods, and sampling volume is accurate, and particle-capture rate is high, light small and exquisite, is very suitable to move
Dynamic detection.
Above-mentioned compensation method is sampled applied to plankton, can meet following standard：1, GMP drug and food produces matter
Measure management regulation；2, the biological pollution control of the toilet ISO14698-1/2 and relevant control environment；3,GB/T 16293-2010
The test method of medical industry toilet (area) flcating germ.
It is to be illustrated to preferable implementation of the invention, but the invention is not limited to the implementation above
Example, those skilled in the art can also make various equivalent variations on the premise of without prejudice to spirit of the invention or replace
It changes, these equivalent deformations or replacement are all included in the scope defined by the claims of the present application.
Claims (10)
1. the flow-compensated system of plankton sampler, which is characterized in that including controlling main module, microprocessor, communication
Module, blower and the air flow sensor being arranged on air flue, the air flow sensor is connect with microprocessor, described
Microprocessor is connect by communication module with control main module, and the control main module is connect with blower；
Flow parameter is sent to micro- by the air flow sensor for acquiring the flow parameter of the air on air flue in real time
Processor；
After the microprocessor is used to obtain blower work time parameter according to preset vol value, by blower work time parameter hair
After sending the flow volume to control main module, and according to flow parameter calculating acquisition, according to flow volume and preset vol value
Blower compensating parameter is obtained, and blower compensating parameter is sent to control main module；
The control main module is used for the working condition according to blower work time parameter and blower compensating parameter control blower.
2. the flow-compensated system of plankton sampler according to claim 1, which is characterized in that the blower
Working condition is divided into three phases, and the three phases, which are followed successively by, accelerates starting period, at the uniform velocity phase and even deceleration withholding period.
3. the flow-compensated system of plankton sampler according to claim 2, which is characterized in that the fanman
It include the second time period that blower work works in the first time period and blower for accelerating the starting period in the at the uniform velocity phase as time parameter,
Described the step of blower work time parameter is obtained according to preset vol value, specially：
According to preset vol value and preset blower running parameter, blower work is calculated separately in the first time for accelerating the starting period
The second time period of section and work in the at the uniform velocity phase；
The preset blower running parameter includes that blower work works in the predetermined acceleration and blower for accelerating the starting period even
The preset flow value of fast phase.
4. the flow-compensated system of plankton sampler according to claim 3, which is characterized in that the blower is mended
Repaying parameter includes third period and acceleration of the blower work in even withholding period of slowing down, described according to flow volume and default body
Product value obtains the step of blower compensating parameter, includes the following steps：
Flow volume is calculated according to flow parameter in real time, and when blower worked to even decelerations withholding period, in conjunction with flow volume with
Preset vol value obtains compensation bulking value；
Third period and acceleration of the blower work in even withholding period of slowing down are obtained according to compensation bulking value and preset flow value.
5. the flow-compensated system of plankton sampler according to claim 4, which is characterized in that the blower is mended
Repaying parameter further includes power adjustment parameters, the work according to blower work time parameter and blower compensating parameter control blower
The step of state, specially：
In first time period, blower is controlled according to predetermined acceleration and is accelerated in first time period；
In second time period, the operating power of blower is adjusted in real time according to power adjustment parameters, make the flow parameter etc. of acquisition
In preset flow value；
Within the third period, uniformly accelerated motion is done according to Acceleration Control blower, until blower stops.
6. the flow-compensated system of plankton sampler according to claim 1, which is characterized in that further include control
Panel, the control panel are connect with control main module.
7. the flow-compensated method of plankton sampler, which is characterized in that include the following steps：
After obtaining preset vol value, blower work time parameter is obtained according to preset vol value；
It is worked according to working time state modulator blower, and in real time after the flow parameter of the air on acquisition air flue, according to
Flow parameter calculates the flow volume of acquisition；
Blower compensating parameter is obtained according to flow volume and preset vol value；
The working condition that blower is controlled according to blower compensating parameter, until blower stops.
8. the flow-compensated method of plankton sampler according to claim 7, which is characterized in that the blower
Working condition is divided into three phases, and the three phases are respectively to accelerate starting period, at the uniform velocity phase and even deceleration withholding period.
9. the flow-compensated method of plankton sampler according to claim 8, which is characterized in that the fanman
It include the second time period that blower work works in the first time period and blower for accelerating the starting period in the at the uniform velocity phase as time parameter,
Described the step of blower work time parameter is obtained according to preset vol value, specially：
According to preset vol value and preset blower running parameter, blower work is calculated separately in the first time for accelerating the starting period
The second time period of section and work in the at the uniform velocity phase；
The preset blower running parameter includes that blower work works in the predetermined acceleration and blower for accelerating the starting period even
The preset flow value of fast phase.
10. the flow-compensated method of plankton sampler according to claim 9, which is characterized in that the blower
Compensating parameter includes third period and acceleration of the blower work in even withholding period of slowing down, described according to flow volume and default
Bulking value obtains the step of blower compensating parameter, includes the following steps：
Flow volume is calculated according to flow parameter in real time, and when blower worked to even decelerations withholding period, in conjunction with flow volume with
Preset vol value obtains compensation bulking value；
Third period and acceleration of the blower work in even withholding period of slowing down are obtained according to compensation bulking value and preset flow value.
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