CN108888639A - A kind of preparation method of Thesium chinese effective component - Google Patents
A kind of preparation method of Thesium chinese effective component Download PDFInfo
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- CN108888639A CN108888639A CN201810741165.2A CN201810741165A CN108888639A CN 108888639 A CN108888639 A CN 108888639A CN 201810741165 A CN201810741165 A CN 201810741165A CN 108888639 A CN108888639 A CN 108888639A
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- preparation
- thesium chinese
- thesium
- eluate
- resin column
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/12—Drugs for disorders of the urinary system of the kidneys
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The invention discloses a kind of preparation methods of Thesium chinese effective component, and steps are as follows：Thesium chinese herb is cut into segment, is cleaned, is decocted 2-4 times with distilled water, each 2.5-3.5h, collecting decoction, stirring, 23-25h is stood after cooling, supernatant through macroporous resin column adsorb 2 times, purifying water washing to eluate it is colourless after, resin column successively use volume fraction be 15%, 25%, 35%, 45%, 55% ethanol gradient elution, 55% ethanol eluate is recovered under reduced pressure, through colloidal silica dispersion, upper silica gel column chromatography is followed successively by 35 with volume ratio:1,24:1,18:1,6:1,1:1 methylene chloride-methanol elution, collects 6:1 eluent, decompression concentration to obtain the final product.This method is easy, quick, easy to operate, and the Thesium chinense of preparation has good Renoprotective Effect, has huge market prospects.
Description
Technical field
The present invention relates to a kind of preparation methods of Thesium chinese effective component.
Background technique
Thesium chinese（Thesium chinensis Turcz）For the perennial parasitic draft of Santalaceae Thesium, alias is thin
Moss, a pine, Green Dragon grass etc., are often attached on the root of other plant and grow, distributed more widely in China, and the main place of production is peace
Emblem, Zhejiang and Hubei etc. save.Thesium chinese is cold in nature, acrid flavour, puckery, and return lung, kidney two warp have the effect of clearing heat and detoxicating, to invigorate the kidney and stop nocturnal emission, tool
Have it is good it is anti-inflammatory, antipyretic, analgesia, antibacterial the effects of.
Summary of the invention
The purpose of the present invention is to provide a kind of preparation methods of Thesium chinese effective component.
The present invention is realized by following technical solution：
A kind of preparation method of Thesium chinese effective component, includes the following steps：Thesium chinese herb is cut into segment, is cleaned, with steaming
Distilled water decocts 2-4 times, each 2.5-3.5h, collecting decoction, and stirring stands 23-25h after cooling, and supernatant is through macroporous resin column
Absorption 2 times, purifying water washing to eluate it is colourless after, resin column successively use volume fraction be 15%, 25%, 35%, 45%, 55% second
55% ethanol eluate is recovered under reduced pressure in alcohol gradient elution, and through colloidal silica dispersion, upper silica gel column chromatography is followed successively by 35 with volume ratio:1,
24:1,18:1,6:1,1:1 methylene chloride-methanol elution, collects 6:1 eluent, decompression concentration to obtain the final product.
Preferably, it in the preparation method, is decocted 2-4 times with distilled water.
Preferably, in the preparation method, each 2.5-3.5h.
Preferably, it in the preparation method, is stood for 24 hours after cooling.
Preferably, in the preparation method, 1kg Thesium chinese is extracted with 9-11L solvent.
Preferably, in the preparation method, the macroreticular resin is AB-8 type.
The technology of the present invention effect：
This method is easy, quick, easy to operate, and the Thesium chinense of preparation has good Renoprotective Effect, has huge
Market prospects.
Specific embodiment
Essentiality content of the invention is specifically introduced below with reference to embodiment.
Embodiment 1
A kind of preparation method of Thesium chinese effective component, includes the following steps：Thesium chinese herb is cut into segment, is cleaned, with steaming
Distilled water decocts 3 times, each 3h, collecting decoction, and stirring is stood for 24 hours after cooling, and supernatant is through AB-8 type macroporous resin column absorption 2
It is secondary, purifying water washing to eluate it is colourless after, resin column successively use volume fraction be 15%, 25%, 35%, 45%, 55% ethanol gradient
Elution, is recovered under reduced pressure 55% ethanol eluate, through colloidal silica dispersion, upper silica gel column chromatography is followed successively by 35 with volume ratio:1,24:1,
18:1,6:1,1:1 methylene chloride-methanol elution, collects 6:1 eluent, decompression concentration to obtain the final product.
Wherein, 1kg Thesium chinese is extracted with 10L solvent.
Embodiment 2
A kind of preparation method of Thesium chinese effective component, includes the following steps：Thesium chinese herb is cut into segment, is cleaned, with steaming
Distilled water decocts 2 times, each 2.5h, collecting decoction, stirring, and 23h is stood after cooling, and supernatant is adsorbed through AB-8 type macroporous resin column
2 times, purifying water washing to eluate it is colourless after, resin column successively use volume fraction be 15%, 25%, 35%, 45%, 55% ethyl alcohol ladder
Degree elution, is recovered under reduced pressure 55% ethanol eluate, through colloidal silica dispersion, upper silica gel column chromatography is followed successively by 35 with volume ratio:1,24:1,
18:1,6:1,1:1 methylene chloride-methanol elution, collects 6:1 eluent, decompression concentration to obtain the final product.
Wherein, 1kg Thesium chinese is extracted with 9L solvent.
Embodiment 3
A kind of preparation method of Thesium chinese effective component, includes the following steps：Thesium chinese herb is cut into segment, is cleaned, with steaming
Distilled water decocts 4 times, each 3.5h, collecting decoction, stirring, and 25h is stood after cooling, and supernatant is adsorbed through AB-8 type macroporous resin column
2 times, purifying water washing to eluate it is colourless after, resin column successively use volume fraction be 15%, 25%, 35%, 45%, 55% ethyl alcohol ladder
Degree elution, is recovered under reduced pressure 55% ethanol eluate, through colloidal silica dispersion, upper silica gel column chromatography is followed successively by 35 with volume ratio:1,24:1,
18:1,6:1,1:1 methylene chloride-methanol elution, collects 6:1 eluent, decompression concentration to obtain the final product.
Wherein, 1kg Thesium chinese is extracted with 11L solvent.
This method is easy, quick, easy to operate, and the Thesium chinense of preparation has good Renoprotective Effect, has
Huge market prospects.
Claims (6)
1. a kind of preparation method of Thesium chinese effective component, it is characterised in that include the following steps：Thesium chinese herb is cut into small
Section is cleaned, and is decocted 2-4 times, each 2.5-3.5h with distilled water, collecting decoction, and stirring stands 23-25h, supernatant after cooling
Through macroporous resin column adsorb 2 times, purifying water washing to eluate it is colourless after, resin column successively use volume fraction be 15%, 25%,
35%, 55% ethanol eluate is recovered under reduced pressure in 45%, 55% ethanol gradient elution, and through colloidal silica dispersion, upper silica gel column chromatography uses volume
Than being followed successively by 35:1,24:1,18:1,6:1,1:1 methylene chloride-methanol elution, collects 6:1 eluent, decompression concentration are
?.
2. preparation method according to claim 1, it is characterised in that：It is decocted 2-4 times with distilled water.
3. preparation method according to claim 2, it is characterised in that：Each 2.5-3.5h.
4. preparation method according to claim 1, it is characterised in that：It is stood for 24 hours after cooling.
5. preparation method according to claim 1, it is characterised in that：1kg Thesium chinese is extracted with 9-11L solvent.
6. preparation method according to claim 1, it is characterised in that：The macroreticular resin is AB-8 type.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810741165.2A CN108888639A (en) | 2018-07-08 | 2018-07-08 | A kind of preparation method of Thesium chinese effective component |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810741165.2A CN108888639A (en) | 2018-07-08 | 2018-07-08 | A kind of preparation method of Thesium chinese effective component |
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| Publication Number | Publication Date |
|---|---|
| CN108888639A true CN108888639A (en) | 2018-11-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810741165.2A Withdrawn CN108888639A (en) | 2018-07-08 | 2018-07-08 | A kind of preparation method of Thesium chinese effective component |
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| CN (1) | CN108888639A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115947770A (en) * | 2022-12-26 | 2023-04-11 | 安徽省森湶谷药业股份有限公司 | Novel thesine compound and application thereof |
-
2018
- 2018-07-08 CN CN201810741165.2A patent/CN108888639A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115947770A (en) * | 2022-12-26 | 2023-04-11 | 安徽省森湶谷药业股份有限公司 | Novel thesine compound and application thereof |
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Application publication date: 20181127 |