CN111214527A - Preparation method of flavonoid component in radix rhapontici - Google Patents
Preparation method of flavonoid component in radix rhapontici Download PDFInfo
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- CN111214527A CN111214527A CN201811414717.5A CN201811414717A CN111214527A CN 111214527 A CN111214527 A CN 111214527A CN 201811414717 A CN201811414717 A CN 201811414717A CN 111214527 A CN111214527 A CN 111214527A
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- radix rhapontici
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The invention discloses a preparation method of flavonoid components in radix rhapontici, which comprises the following steps: pulverizing radix Rhapontici of Yuzhou, extracting with 85-95% ethanol flow for 2-4 times (1.5-2.5 hr each time), filtering, mixing extractive solutions, and concentrating under reduced pressure to obtain extract; dissolving the extract with appropriate amount of water, sequentially extracting with petroleum ether, chloroform, ethyl acetate and water saturated n-butanol to obtain chloroform fraction; separating chloroform part with silica gel column chromatography, sequentially eluting with petroleum ether-acetone at volume ratio of 52:1, 34:1, 21:1, and 5:1, collecting 21:1 eluate, and concentrating. The preparation method provided by the invention is simple and feasible, can enrich flavonoid components in the radix rhapontici, has a remarkable anti-tumor effect, and can be used as an effective part of the radix rhapontici to be applied to medicine preparation.
Description
Technical Field
The invention relates to a preparation method of flavonoid components in radix rhapontici.
Background
Yuzhou uniflower swisscentaury root is the dry root of the Lansium hybridum head or the Lansium huadong Lanceolatum head of the Compositae, is cold in nature and bitter in taste, has the effects of clearing heat and removing toxicity, eliminating carbuncles, promoting lactation, relaxing tendons and dredging collaterals, and is commonly used for treating acute mastitis, swelling and pain, carbuncle, sore back, scrofula and sore, galactostasis, and damp arthralgia and spasm. Removing impurities, cleaning, moistening, slicing, and sun drying. The product is round or round-like thick tablet. The outer skin is grayish yellow to grayish brown. The section skin part is brown, and the wood part is in yellow-black radial texture. Light smell, slightly astringent taste. The Echinacea purpurea is distributed in the Tianshan area of Xinjiang; the HUAdong Lanceolata is distributed in Liaoning (south), Shandong, Henan, Anhui, Jiangsu, Fujian, Taiwan, and Guangxi.
Disclosure of Invention
The invention aims to provide a preparation method of flavonoid components in radix rhapontici.
The invention is realized by the following technical scheme:
a preparation method of flavonoid components in radix rhapontici comprises the following steps: pulverizing radix Rhapontici of Yuzhou, extracting with 85-95% ethanol flow for 2-4 times (1.5-2.5 hr each time), filtering, mixing extractive solutions, and concentrating under reduced pressure to obtain extract; dissolving the extract with appropriate amount of water, sequentially extracting with petroleum ether, chloroform, ethyl acetate and water saturated n-butanol to obtain chloroform fraction; separating chloroform part with silica gel column chromatography, sequentially eluting with petroleum ether-acetone at volume ratio of 52:1, 34:1, 21:1, and 5:1, collecting 21:1 eluate, and concentrating.
Preferably, in the preparation method, the ethanol flow with the volume fraction of 85-95% is used for extracting for 2-4 times.
Preferably, in the preparation method, 1.5-2.5 hours are carried out each time.
Preferably, in the preparation method, 1kg of the radix rhapontici is extracted by 7-9L of solvent.
Preferably, in the preparation method, 1g of the extract is dissolved by 4-6mL of water.
The invention has the technical effects that:
the preparation method provided by the invention is simple and feasible, can enrich flavonoid components in the radix rhapontici, has a remarkable anti-tumor effect, and can be used as an effective part of the radix rhapontici to be applied to medicine preparation.
Detailed Description
The following describes the substance of the present invention with reference to the examples.
Example 1
A preparation method of flavonoid components in radix rhapontici comprises the following steps: pulverizing radix Rhapontici of Yuzhou, extracting with 90% ethanol flow for 3 times (2 hr each time), filtering, mixing extractive solutions, and concentrating under reduced pressure to obtain extract; dissolving the extract with appropriate amount of water, sequentially extracting with petroleum ether, chloroform, ethyl acetate and water saturated n-butanol to obtain chloroform fraction; separating chloroform part with silica gel column chromatography, sequentially eluting with petroleum ether-acetone at volume ratio of 52:1, 34:1, 21:1, and 5:1, collecting 21:1 eluate, and concentrating.
Wherein, 1kg of Yuzhou uniflower swisscentaury root is extracted by 8L of solvent, and 1g of extract is dissolved by 5mL of water.
Example 2
A preparation method of flavonoid components in radix rhapontici comprises the following steps: pulverizing radix Rhapontici of Yuzhou, extracting with 85% ethanol flow for 2 times (1.5 hr each time), filtering, mixing extractive solutions, and concentrating under reduced pressure to obtain extract; dissolving the extract with appropriate amount of water, sequentially extracting with petroleum ether, chloroform, ethyl acetate and water saturated n-butanol to obtain chloroform fraction; separating chloroform part with silica gel column chromatography, sequentially eluting with petroleum ether-acetone at volume ratio of 52:1, 34:1, 21:1, and 5:1, collecting 21:1 eluate, and concentrating.
Wherein, 1kg of Yuzhou uniflower swisscentaury root is extracted by 7L of solvent, and 1g of extract is dissolved by 4mL of water.
Example 3
A preparation method of flavonoid components in radix rhapontici comprises the following steps: pulverizing radix Rhapontici of Yuzhou, extracting with 95% ethanol flow for 4 times (2.5 hr each time), filtering, mixing extractive solutions, and concentrating under reduced pressure to obtain extract; dissolving the extract with appropriate amount of water, sequentially extracting with petroleum ether, chloroform, ethyl acetate and water saturated n-butanol to obtain chloroform fraction; separating chloroform part with silica gel column chromatography, sequentially eluting with petroleum ether-acetone at volume ratio of 52:1, 34:1, 21:1, and 5:1, collecting 21:1 eluate, and concentrating.
Wherein, 1kg of Yuzhou uniflower swisscentaury root is extracted by 9L of solvent, and 1g of extract is dissolved by 6mL of water.
The preparation method provided by the invention is simple and feasible, can enrich flavonoid components in the radix rhapontici, has a remarkable anti-tumor effect, and can be used as an effective part of the radix rhapontici to be applied to medicine preparation.
Claims (5)
1. A preparation method of flavonoid components in radix rhapontici is characterized by comprising the following steps: pulverizing radix Rhapontici of Yuzhou, extracting with 85-95% ethanol flow for 2-4 times (1.5-2.5 hr each time), filtering, mixing extractive solutions, and concentrating under reduced pressure to obtain extract; dissolving the extract with appropriate amount of water, sequentially extracting with petroleum ether, chloroform, ethyl acetate and water saturated n-butanol to obtain chloroform fraction; separating chloroform part with silica gel column chromatography, sequentially eluting with petroleum ether-acetone at volume ratio of 52:1, 34:1, 21:1, and 5:1, collecting 21:1 eluate, and concentrating.
2. The method of claim 1, wherein: extracting with 85-95% ethanol stream for 2-4 times.
3. The method of claim 1, wherein: each time for 1.5-2.5 h.
4. The method of claim 1, wherein: 1kg of radix Rhapontici is extracted with 7-9L of solvent.
5. The method of claim 1, wherein: 1g of the extract is dissolved by 4-6mL of water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811414717.5A CN111214527A (en) | 2018-11-26 | 2018-11-26 | Preparation method of flavonoid component in radix rhapontici |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811414717.5A CN111214527A (en) | 2018-11-26 | 2018-11-26 | Preparation method of flavonoid component in radix rhapontici |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111214527A true CN111214527A (en) | 2020-06-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811414717.5A Pending CN111214527A (en) | 2018-11-26 | 2018-11-26 | Preparation method of flavonoid component in radix rhapontici |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111214527A (en) |
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2018
- 2018-11-26 CN CN201811414717.5A patent/CN111214527A/en active Pending
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20200602 |
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| WD01 | Invention patent application deemed withdrawn after publication |