CN108969551A - The preparation method of lipid-loweringing ingredient in a kind of dichromatism golden wave - Google Patents
The preparation method of lipid-loweringing ingredient in a kind of dichromatism golden wave Download PDFInfo
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- CN108969551A CN108969551A CN201810741251.3A CN201810741251A CN108969551A CN 108969551 A CN108969551 A CN 108969551A CN 201810741251 A CN201810741251 A CN 201810741251A CN 108969551 A CN108969551 A CN 108969551A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/06—Antihyperlipidemics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The invention discloses a kind of preparation methods of lipid-loweringing ingredient in dichromatism golden wave, steps are as follows: dichromatism golden wave medicinal material is ground, is that 60-70% EtOH Sonicate extracts 2-4 times with volume fraction, each 30-40min, combined extract, extracting solution are concentrated under reduced pressure to give medicinal extract；Petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol are successively used after medicinal extract is dissolved with suitable quantity of water, and acetic acid ethyl ester extract is concentrated under reduced pressure to obtain；By acetic acid ethyl ester extract large pore resin absorption column chromatographic isolation, successively with pure water, the ethanol gradient elution of volume fraction 10%, 30%, 50%, 70%, 95%, collect 70% alcohol elution, it is separated again through silica gel column chromatography, successively eluted with the chloroform-methanol of volume ratio 35:1,25:1,15:1,5:1, it collects 15:1 elution fraction, is concentrated to obtain the final product.The preparation method is simple and easy to operate, can effectively extract lipid-loweringing ingredient in dichromatism golden wave.
Description
Technical field
The present invention relates to a kind of preparation methods of lipid-loweringing ingredient in dichromatism golden wave.
Background technique
Dichromatism golden wave also known as coreopsis tinctoria are annual herb plant Coreopsis tinctoriaSanvitalia procumbens
Lam. dry capitulum, golden pheasant Chrysanthemum herbaceos perennial, class resist cold drought-enduring, require soil not tight, light, but resistance to
Half yin, it is adaptable, there is stronger resistance to sulfur dioxide, originates in the ornamental plant of North America.The common cultivation in China various regions.Have
The effect of clearing heat and detoxicating, activating microcirculation and removing stasis medicinal, stomach function regulating invigorating the spleen.Modern pharmacological studies have shown that dichromatism golden wave has anti-inflammatory, decompression, drop
Rouge, hypoglycemic, anticoagulation, anti-aging, it is antiviral the effects of.
Summary of the invention
The purpose of the present invention is to provide a kind of preparation methods of lipid-loweringing ingredient in dichromatism golden wave.
The present invention is realized by following technical solution:
The preparation method of lipid-loweringing ingredient in a kind of dichromatism golden wave includes the following steps: to grind dichromatism golden wave medicinal material, crosses 3
Number sieve, with volume fraction be 60-70% EtOH Sonicate extract 2-4 time, each 30-40min, combined extract, extracting solution depressurize it is dense
Contracting obtains medicinal extract；Petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol, decompression are successively used after medicinal extract is dissolved with suitable quantity of water
It is concentrated to give acetic acid ethyl ester extract；By acetic acid ethyl ester extract large pore resin absorption column chromatographic isolation, pure water, volume are successively used
The ethanol gradient elution of score 10%, 30%, 50%, 70%, 95% is collected 70% alcohol elution, then is separated through silica gel column chromatography,
It is successively eluted with the chloroform-methanol of volume ratio 35:1,25:1,15:1,5:1, collects 15:1 elution fraction, be concentrated to obtain the final product.
It preferably, is that 65% EtOH Sonicate extracts 3 times with volume fraction in the preparation method.
Preferably, in the preparation method, each 35min.
Preferably, in the preparation method, 1kg dichromatism golden wave is extracted using 7-9L solvent.
Preferably, in the preparation method, the large pore resin absorption column is AB-8 type.
The technology of the present invention effect:
The preparation method is simple and easy to operate, can effectively extract lipid-loweringing ingredient in dichromatism golden wave, can be prepared on a large scale.
Specific embodiment
Essentiality content of the invention is specifically introduced below with reference to embodiment.
Embodiment 1
The preparation method of lipid-loweringing ingredient in a kind of dichromatism golden wave includes the following steps: to grind dichromatism golden wave medicinal material, crosses 3
Number sieve, with volume fraction be 65% EtOH Sonicate extract 3 times, each 35min, combined extract, extracting solution is concentrated under reduced pressure to give leaching
Cream；Petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol are successively used after medicinal extract is dissolved with suitable quantity of water, and second is concentrated under reduced pressure to obtain
Acetoacetic ester extract；By acetic acid ethyl ester extract AB-8 type large pore resin absorption column chromatographic isolation, successively with pure water, volume point
The ethanol gradient elution of number 10%, 30%, 50%, 70%, 95% is collected 70% alcohol elution, then is separated through silica gel column chromatography, according to
The secondary chloroform-methanol with volume ratio 35:1,25:1,15:1,5:1 elutes, and collects 15:1 elution fraction, is concentrated to obtain the final product.
Wherein, 1kg dichromatism golden wave is extracted using 8 solvents.
Embodiment 2
The preparation method of lipid-loweringing ingredient in a kind of dichromatism golden wave includes the following steps: to grind dichromatism golden wave medicinal material, crosses 3
Number sieve, with volume fraction be 60% EtOH Sonicate extract 2 times, each 30min, combined extract, extracting solution is concentrated under reduced pressure to give leaching
Cream；Petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol are successively used after medicinal extract is dissolved with suitable quantity of water, and second is concentrated under reduced pressure to obtain
Acetoacetic ester extract；By acetic acid ethyl ester extract AB-8 type large pore resin absorption column chromatographic isolation, successively with pure water, volume point
The ethanol gradient elution of number 10%, 30%, 50%, 70%, 95% is collected 70% alcohol elution, then is separated through silica gel column chromatography, according to
The secondary chloroform-methanol with volume ratio 35:1,25:1,15:1,5:1 elutes, and collects 15:1 elution fraction, is concentrated to obtain the final product.
Wherein, 1kg dichromatism golden wave is extracted using 7L solvent.
Embodiment 3
The preparation method of lipid-loweringing ingredient in a kind of dichromatism golden wave includes the following steps: to grind dichromatism golden wave medicinal material, crosses 3
Number sieve, with volume fraction be 70% EtOH Sonicate extract 4 times, each 40min, combined extract, extracting solution is concentrated under reduced pressure to give leaching
Cream；Petroleum ether, ethyl acetate and water saturated extracting n-butyl alcohol are successively used after medicinal extract is dissolved with suitable quantity of water, and second is concentrated under reduced pressure to obtain
Acetoacetic ester extract；By acetic acid ethyl ester extract AB-8 type large pore resin absorption column chromatographic isolation, successively with pure water, volume point
The ethanol gradient elution of number 10%, 30%, 50%, 70%, 95% is collected 70% alcohol elution, then is separated through silica gel column chromatography, according to
The secondary chloroform-methanol with volume ratio 35:1,25:1,15:1,5:1 elutes, and collects 15:1 elution fraction, is concentrated to obtain the final product.
Wherein, 1kg dichromatism golden wave is extracted using 9L solvent.
The preparation method is simple and easy to operate, can effectively extract lipid-loweringing ingredient in dichromatism golden wave, can be prepared on a large scale.
Claims (5)
1. the preparation method of lipid-loweringing ingredient in a kind of dichromatism golden wave, it is characterised in that include the following steps: dichromatism golden wave
Medicinal material is ground, and crosses No. 3 sieves, is that 60-70% EtOH Sonicate extracts 2-4 times, each 30-40min with volume fraction, combined extract,
Extracting solution is concentrated under reduced pressure to give medicinal extract；After medicinal extract is dissolved with suitable quantity of water successively with petroleum ether, ethyl acetate and it is water saturated just
Acetic acid ethyl ester extract is concentrated under reduced pressure to obtain in butanol, before immunoassay；By acetic acid ethyl ester extract large pore resin absorption column chromatographic isolation, according to
Secondary pure water, the ethanol gradient elution of volume fraction 10%, 30%, 50%, 70%, 95% collect 70% alcohol elution, then through silicon
Rubber column gel column chromatographic isolation is successively eluted with the chloroform-methanol of volume ratio 35:1,25:1,15:1,5:1, collects 15:1 elution fraction,
It is concentrated to obtain the final product.
2. preparation method according to claim 1, it is characterised in that: extracted 3 times with volume fraction for 65% EtOH Sonicate.
3. preparation method according to claim 2, it is characterised in that: each 35min.
4. preparation method according to claim 1, it is characterised in that: 1kg dichromatism golden wave is mentioned using 7-9L solvent
It takes.
5. preparation method according to claim 1, it is characterised in that: the large pore resin absorption column is AB-8 type.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810741251.3A CN108969551A (en) | 2018-07-08 | 2018-07-08 | The preparation method of lipid-loweringing ingredient in a kind of dichromatism golden wave |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810741251.3A CN108969551A (en) | 2018-07-08 | 2018-07-08 | The preparation method of lipid-loweringing ingredient in a kind of dichromatism golden wave |
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| Publication Number | Publication Date |
|---|---|
| CN108969551A true CN108969551A (en) | 2018-12-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810741251.3A Withdrawn CN108969551A (en) | 2018-07-08 | 2018-07-08 | The preparation method of lipid-loweringing ingredient in a kind of dichromatism golden wave |
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| CN (1) | CN108969551A (en) |
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2018
- 2018-07-08 CN CN201810741251.3A patent/CN108969551A/en not_active Withdrawn
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Application publication date: 20181211 |