CN1088750C - Method for brewing health-care beer by secodary fermentation - Google Patents
Method for brewing health-care beer by secodary fermentation Download PDFInfo
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- CN1088750C CN1088750C CN99112741A CN99112741A CN1088750C CN 1088750 C CN1088750 C CN 1088750C CN 99112741 A CN99112741 A CN 99112741A CN 99112741 A CN99112741 A CN 99112741A CN 1088750 C CN1088750 C CN 1088750C
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- cordyceps militaris
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Abstract
The present invention relates to a method which utilizes fungus fermentation liquor, such as cordyceps, etc., as a nutrient source of beer yeast to prepare beer which has health care efficiency in a secondary fermentation mode. The method uses malt wort or malt mixed juice used for preparing beer as a culture medium for the first fermentation. Fungus, such as mycelium of cordyceps, etc., is fermented and cultured in deep liquid in advance. After the first fermentation is completed, fermentation liquid is separated and saccharified, a pH value is adjusted, and the secondary fermentation of the beer yeast is carried out. Products of health beer which contains nutrient components of the cordyceps are prepared. The method adopts raw material of beer to prepare fungus, such as cordyceps, etc., and cordyceps beer, resources are fully utilized, and the beer has the health care function for enhancing the non-specificity immunity of a human body.
Description
The present invention relates to a kind of method of utilizing brewing health-care beer by secodary fermentation.
Beer is as a kind of popular consumer goods, because its fragrant hardship is clearly agreeable to the taste, alcoholic strength is low and well received.The production method of traditional various beer is all made as single microorganism one time fermentation method by cereuisiae fermentum.Its mode of production is:
At first the ratio that requires according to beer production is boiled saccharification with Fructus Hordei Germinatus and auxiliary material, and preparation wort (or Fructus Hordei Germinatus mixing juice) adds in the boiling pot, transfers PH, regulates concentration.Be heated to and boil, add the hops several times.Boiled 90 minutes, and be about to feed liquid precipitation, cooling, remove reduce phlegm and internal heat, cold sludge.Logical simultaneously sterile air makes the feed liquid oxygen level reach manufacturing requirements.
After feed liquid is gone into fermentor tank, add yeast and carry out the normal pressure fermentation.Regularly survey pol, survey the product temperature, survey di-acetyl.When di-acetyl is reduced to 0.15ppm/L, will product temperature drop to 5 ℃, kept this temperature 24 hours, row's yeast secondary.Afterwards again will product temperature drop to 0 ℃, kept this temperature 24 hours, row's yeast 2～3 times.Kept pressure, temperature storage wine 15 days, and filtered, make finished beer through filter.
On the other hand Cordyceps microorganisms such as Cordyceps militaris (L.) Link., glossy ganoderma, hedgehog hydnum, needle mushroom be China's preciousness medicine, eat dual-purpose fungi.Except that itself having very high value, their nutrient solution also contains many nutritive ingredients.Wherein contain fungus polysaccharides such as Cordyceps polysaccharide, cordycepin and cordycepic acid in Cordyceps militaris (L.) Link. (Cordycepsmilitaris (L.) Link) fermented liquid, it is a kind of wide spectrum immunopotentiating agent, can improve cellular immunization, strengthen humoral immunization, and the effect that suppresses tumour is arranged.Some fungus polysaccharide can also strengthen human body to various bacteriums, virus, fungi and parasitic resistibility.Cor pulmonale and coronary heart disease all there are certain curative effect, have very high medicinal health value.But because Cordyceps militaris (L.) Link. is cultivated small scale, and its purpose is mainly used to cultivate thalline, and fermented liquid is not generally cooked utilization, therefore also do not have suitability for industrialized production.
Purpose of the present invention is exactly that cultivation and beer production with fungies such as Cordyceps militaris (L.) Link. combines, and forms a kind of production method of new health-care beer.
The present invention is to be that raw material is cultivated radicula byssoideas such as Cordyceps militaris (L.) Link. with beer production with wort or Fructus Hordei Germinatus mixing juice, after finishing deep fermentation, fermented liquid is through separating, replenish links such as wort, adjusting concentration, accent PH, carry out the Secondary Fermentation of cereuisiae fermentum, produce the health-care beer product that contains the caterpillar fungus nutritire composition.
The present invention at first prepares wort (or Fructus Hordei Germinatus mixing juice) according to the ratio that beer production requires.Sterilization is cooled to 20～26 ℃, pumps into ventilation fermentation tank, inoculation 5～20% Cordyceps militaris spawns (calculating) with the wort amount, and ventilation stir culture fermentation 72～96 hours, centrifugation, separating the clear liquid that obtains is Chinese caterpillar fungus one time fermentation liquid.
In one time fermentation, to solve two problems.Because beer mostly is big suitability for industrialized production, the scale of Cordyceps militaris (L.) Link. one time fermentation must match with the industrial scale of beer on the one hand.The beer production of Secondary Fermentation is the liquid fermenting production process on the other hand.This just requires one time fermentation adapting with Secondary Fermentation aspect industrial scale and the production status two.The present invention is applied in the Cordyceps militaris (L.) Link. one time fermentation with the deep fermentation technology and cultivates the continuous ventilating enlarged culturing for this reason.Not only solved the problems referred to above, and made the production of Cordyceps militaris (L.) Link. realize industrialization.
One time fermentation separates the Cordyceps militaris (L.) Link. fungus filament after finishing with fermented liquid.Cordyceps militaris (L.) Link. can be used as medicine or healthcare products raw material after treatment.
Cordyceps militaris (L.) Link. one time fermentation clear liquid after the separation is transferred PH, regulates concentration.Be heated to and boil, add the hops several times.Boiled 90 minutes, and be about to feed liquid precipitation, cooling, remove reduce phlegm and internal heat, cold sludge.Logical simultaneously sterile air makes the feed liquid oxygen level reach manufacturing requirements.
After feed liquid is gone into fermentor tank, add yeast and carry out the normal pressure fermentation.Regularly survey pol, sealed cans nature increasing temperature and pressure then, when di-acetyl is reduced to 0.15ppm/L, will product temperature drop to 5 ℃, kept this temperature 24 hours, arrange the yeast secondary.Afterwards again will product temperature drop to 0 ℃, kept this temperature 24 hours, row's yeast 2～3 times.Kept pressure, temperature storage wine 15 days, and filtered, make finished product Cordyceps militaris (L.) Link. beer through diatomite filter.
The present invention makes full use of the unnecessary nutritive ingredient in the wort, can not only obtain a large amount of Cordyceps militaris (L.) Link. fungus filaments, and polysaccharide that Cordyceps militaris (L.) Link. generates in when fermentation etc. is secreted in the fermented liquid human body beneficial's composition, and allow cordyceps sinensis fermentation liquor obtain fully effectively utilizing, carry out Secondary Fermentation.Make Chinese caterpillar fungus beer not only have the local flavor and the function of beer, but also contain the composition that Cordyceps militaris (L.) Link. polysaccharide etc. can improve the human body non-specific immunity, can improve function of human body and health level after drinking.This method also adopts the deep fermentation technology to produce Cordyceps militaris (L.) Link., make it to adapt with brewing industry production, and the industrialization of producing for Cordyceps militaris (L.) Link. self provides new mode.
This method has added the aerobic fermentation of Cordyceps militaris (L.) Link. on the basis of original beer technology.Make the polysaccharide that beer product is rich in can increase immunologic function, have obvious health care effect.Enforcement of the present invention needs to be added with aerobe fermentation equipment on original production equipment, increase controlled variable.Cost is than the traditional technology height.But the present invention makes full use of raw material, and the one-time process process can be produced Chinese caterpillar fungus and two kinds of products of Chinese caterpillar fungus beer.Two kinds of products have very high added value and market potential.
Specific embodiment is as follows:
1, produce 3 tons of worts, sterilization is cooled to 22 ℃, pump into ventilation fermentation tank after, inoculate 10% Cordyceps militaris (L.) Link. thalline (calculating) with the wort amount, ventilate and stirred fermentation culture 72 hours, centrifugation, separating the clear liquid that obtains is Chinese caterpillar fungus one time fermentation liquid.One time fermentation liquid is added boiling pot.
Transfer about PH to 5.4, transfer wort concentration.Being heated to boils promptly adds Bitters and spends 0.7 ‰, when boiling 80 minutes, adds Bitters again and spends 0.3 ‰.Boil about 10 minutes, make original wort concentration reach 12%, be about to feed liquid and add the vortex settling bath.Precipitate 30 minutes, begin to cool down.The material temperature control is at 6.5～7.0 ℃, and logical simultaneously sterile air makes the feed liquid oxygen level reach 8mg/L.
After feed liquid is gone into fermentor tank, add yeast 1% and carry out the normal pressure fermentation.Survey pol every day once, sealed cans when pol reduces to 5.5%～6%.Secondary Fermentation liquid is warming up to 12 ℃ naturally, boosts to 0.01～0.14MPa, when di-acetyl is reduced to 0.15ppm/L, with every day 0.3 ℃ cooling rate will product temperature drop to 5 ℃, kept this temperature 24 hours, row's yeast secondary.Afterwards will product temperature drop to 0 ℃, kept this temperature 24 hours, row's yeast 2～3 times.Keep pressure 0.05～0.08MPa, temperature is filtered through diatomite filter to-1～0 ℃ of storage wine 15 days, makes Cordyceps militaris (L.) Link. beer.
2, produce 3 tons of worts, sterilization is cooled to 20 ℃, pump into ventilation fermentation tank after, inoculate 20% Cordyceps militaris (L.) Link. thalline (calculating) with the wort amount, ventilate and stirred fermentation culture 96 hours, centrifugation, separating the clear liquid that obtains is Chinese caterpillar fungus one time fermentation liquid.One time fermentation liquid is added boiling pot.
Transfer about PH to 5.4, transfer wort concentration.Being heated to boils promptly adds Bitters and spends 0.7 ‰, when boiling 80 minutes, adds Bitters again and spends 0.3 ‰.Boil about 10 minutes, make original wort concentration reach 12%, be about to feed liquid and add the vortex settling bath.Precipitate 30 minutes, begin to cool down.The material temperature control is at 6.5～7.0 ℃, and logical simultaneously sterile air makes the feed liquid oxygen level reach 8mg/L.
After feed liquid is gone into fermentor tank, add yeast 1% and carry out the normal pressure fermentation.Survey pol every day once, sealed cans when pol reduces to 5.5%～6%.Secondary Fermentation liquid is warming up to 12 ℃ naturally, boosts to 0.01～0.14MPa, when di-acetyl is reduced to 0.15ppm/L, with every day 0.3 ℃ cooling rate will product temperature drop to 5 ℃, kept this temperature 24 hours, row's yeast secondary.Afterwards will product temperature drop to 0 ℃, kept this temperature 24 hours, row's yeast 2～3 times.Keep pressure 0.05～0.08MPa, temperature is filtered through diatomite filter to-1～0 ℃ of storage wine 15 days, makes Cordyceps militaris (L.) Link. beer.
3, produce 3 tons of worts, sterilization is cooled to 26 ℃, pump into ventilation fermentation tank after, inoculate 5% Cordyceps militaris (L.) Link. thalline (calculating) with the wort amount, ventilate and stirred fermentation culture 84 hours, centrifugation, separating the clear liquid that obtains is Chinese caterpillar fungus one time fermentation liquid.One time fermentation liquid is added boiling pot.
Transfer about PH to 5.4, transfer wort concentration.Being heated to boils promptly adds Bitters and spends 0.7 ‰, when boiling 80 minutes, adds Bitters again and spends 0.3 ‰.Boil about 10 minutes, make original wort concentration reach 12%, be about to feed liquid and add the vortex settling bath.Precipitate 30 minutes, begin to cool down.The material temperature control is at 6.5～7.0 ℃, and logical simultaneously sterile air makes the feed liquid oxygen level reach 8mg/L.
After feed liquid is gone into fermentor tank, add yeast 1% and carry out the normal pressure fermentation.Survey pol every day once, sealed cans when pol reduces to 5.5%～6%.Secondary Fermentation liquid is warming up to 12 ℃ naturally, boosts to 0.01～0.14MPa, when di-acetyl is reduced to 0.15ppm/L, with every day 0.3 ℃ cooling rate will product temperature drop to 5 ℃, kept this temperature 24 hours, row's yeast secondary.Afterwards will product temperature drop to 0 ℃, kept this temperature 24 hours, row's yeast 2～3 times.Keep pressure 0.05～0.08MPa, temperature is filtered through diatomite filter to-1～0 ℃ of storage wine 15 days, makes Cordyceps militaris (L.) Link. beer.
Claims (2)
1, a kind of method of producing health-care beer, it is characterized by: wort or the Fructus Hordei Germinatus mixing juice used with beer production are nutrition source, cultivate the Cordyceps militaris (L.) Link. thalline earlier, finish the Cordyceps militaris (L.) Link. fermentation, promptly after the fermentation for the first time, fermented liquid separates, and replenishes wort, adjusts concentration, adjusts PH, be prepared into beer raw material, carry out beer yeast fermenting, promptly the health-care beer product that contains the Cordyceps militaris (L.) Link. polysaccharide component is produced in fermentation for the second time.
2, according to the method for the described production health-care beer of claim 1, it is characterized by: cultivate the mycelial method of Cordyceps militaris (L.) Link. earlier, promptly adopt and inoculate 5%～20% Cordyceps militaris (L.) Link. in the wort, ventilate in 20～26 ℃ and cultivated 72～96 hours, deep fermentation is produced the Cordyceps militaris (L.) Link. fungus filament.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99112741A CN1088750C (en) | 1999-03-11 | 1999-03-11 | Method for brewing health-care beer by secodary fermentation |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99112741A CN1088750C (en) | 1999-03-11 | 1999-03-11 | Method for brewing health-care beer by secodary fermentation |
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| CN1266889A CN1266889A (en) | 2000-09-20 |
| CN1088750C true CN1088750C (en) | 2002-08-07 |
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| CN99112741A Expired - Fee Related CN1088750C (en) | 1999-03-11 | 1999-03-11 | Method for brewing health-care beer by secodary fermentation |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101020872B (en) * | 2006-02-13 | 2011-01-26 | 富阳 | Aweto beer and its making process |
| CN101565662B (en) * | 2008-04-23 | 2012-01-11 | 济南百脉泉啤酒饮品有限公司 | Preparation method of beer by secondary fermentation in vessel |
| CN101560268B (en) * | 2009-05-31 | 2013-11-20 | 江西济民可信集团有限公司 | Cs-4 fermentation mycelium polysaccharide and preparation method and applications thereof |
| CN105018262A (en) * | 2014-04-15 | 2015-11-04 | 温州大学 | Cordyceps militaris draft beer and brewing process thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1101571A (en) * | 1993-10-15 | 1995-04-19 | 张显科 | Technology for producing cordyceps militaris series products |
| CN1112839A (en) * | 1995-04-10 | 1995-12-06 | 李江 | Prepn. method of medical liquor for improving sexual function |
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1999
- 1999-03-11 CN CN99112741A patent/CN1088750C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1101571A (en) * | 1993-10-15 | 1995-04-19 | 张显科 | Technology for producing cordyceps militaris series products |
| CN1112839A (en) * | 1995-04-10 | 1995-12-06 | 李江 | Prepn. method of medical liquor for improving sexual function |
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| CN1266889A (en) | 2000-09-20 |
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