CN108872341B - A kind of preparation and application of the alpha-fetoprotein optical electro-chemistry competition immunologic pattern sensor based on Lazer's structure - Google Patents
A kind of preparation and application of the alpha-fetoprotein optical electro-chemistry competition immunologic pattern sensor based on Lazer's structure Download PDFInfo
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Abstract
The present invention discloses the preparation and application of a kind of alpha-fetoprotein optical electro-chemistry competition immunologic pattern sensor based on Lazer's structure.The construction method of the sensing interface is using titanium dioxide mesomorphic as probe base and fixed alpha-fetoprotein antibody (Ab), using bismuth sulfide as electrode basement, fixed alpha-fetoprotein antigen, a kind of sensor of Lazer's structure is constructed by the specific binding between antigen and antibody.The electrode for being modified with alpha-fetoprotein antigen is put into the mixed solution containing various concentration free antigen and a certain amount of label probe, free target antigen and immobilized antigen competitive binding label probe are made.Under illumination condition, the electrode for combining probe can generate the photoelectric current of some strength, realize the amplification to current signal by Lazer's structure.And with the increase of free target concentration, the quantity of immobilized antigen bonding probes reduces photo-current intensity and also reduces therewith.The photoelectric analysis method for alpha-fetoprotein can be set up based on the phenomenon.
Description
Technical field
The invention belongs to new function materials and bio-sensing detection technique field, and in particular to one kind is tied based on Lazerization
The preparation and application of the alpha-fetoprotein optical electro-chemistry competition immunologic pattern sensor of structure.
Background technique
Optical electro-chemistry (PEC) detection is using light as excitation signal, using photoelectric current as detection signal, by using not
Energy with form makes to excite and detection signal is not interfere with each other as excitation signal and detection signal, thus background signal is lower,
It can get higher sensitivity.In the building process of optical electro-chemistry sensor, response of the selection of light-sensitive material for signal
It is most important, in presently used material, TiO2Nano material is because of its unique photocatalytic activity, nontoxicity, excellent chemistry
And physical stability, become the ideal material of photocatalysis and optical electro-chemistry sensor.TiO2The sight crystal that is situated between is that crystal is sub- single
What first ordered arrangement was constituted, compared to traditional TiO2Monocrystalline, TiO2Mesomorphic has more excellent solar energy conversion and catalytic
Can, PEC performance can be significantly improved.However, TiO2Forbidden bandwidth is larger, can only be by ultraviolet excitation, therefore in visible region light
Photoelectric transformation efficiency is lower.Bi2S3There is excellent photoelectric sensing characteristic compared with semiconductor as a kind of narrow band gap, by itself and TiO2Coupling
Conjunction can expand light absorption wavelength range, promote the separation of photo-generate electron-hole pair, to improve photoelectric current transfer efficiency.
Alpha-fetoprotein (AFP), major plasma proteins and primary as human liver, yolk bag and Gastrointestinal Tract of Fetus
The high specific of liver cancer and highly sensitive tumor markers.The raising of AFP concentration is typically considered prediction and examines in adult blood plasma
Break the early stage indexs of certain Cancerous diseases, including hepatocellular carcinoma, entoderm cancer, carcinoma of testis, teratoma, yolk sac carcinoma, oophoroma
With Gastric cancer with liver metastasis etc..Therefore the sensitive detection method for developing a kind of alpha-fetoprotein has become the side that researchers are studied
To.Immunoassay analysis has good sensitivity and specificity, is usually used in rapid quantitative detection object.This method
Core is mainly antibody and corresponding antigen, through specific recognition site in conjunction with detection substance.And it is most of traditional
PEC immunologic detection method only introduces single light active material, and there is certain limitations, such as light to inhale in actual application
Receipts are limited in scope, and can not effectively prevent the compound of photo-generate electron-hole pair, and photoelectric current transfer efficiency is low etc..The sensor draws
Enter two kinds of light active materials, constructing a kind of Lazer's structure can not only effectively contain that photohole-electronics pair is compound, expand
Light abstraction width takes full advantage of luminous energy, improves photoelectric current transfer efficiency to realize further putting to photo-signal
The big and highly sensitive detection to alpha-fetoprotein.In addition, the sensor two kinds of light active materials are kept completely separate and pass through antigen with
Immune response between antibody combines, and the construction process that this " signal is opened " type sensing modes simplify sensor also subtracts
Interference between small different component.
TiO2Mesomorphic can fix more antibody as probe base, big specific surface area；High-crystallinity and orderly
Three meso-hole structures are able to achieve the multiple reflections to light, to enhance the response of photoelectric current.Bi simultaneously2S3Nanometer sheet is as substrate
Material is fixed on glassy carbon electrode surface using its good electric conductivity, light induced electron can be made preferably to be transmitted to electrode surface.This
When two kinds of light active materials can be combined by the immune response between original antibody and construct a kind of Lazer's structure,
Photo-signal is further amplified in realization.By the free alpha-fetoprotein standard solution of various concentration and a certain number of labels
Probe mixes and the electrode for being modified with alpha-fetoprotein is put into the mixed liquor, makes the alpha-fetoprotein and free first tire egg of immobilization
White competitive binding label probe, with being continuously increased for free alpha-fetoprotein concentration, the position of immobilization alpha-fetoprotein bonding probes
Point gradually decreases, and photocurrent response also reduces therewith, and linear to realize the Gao Ling to alpha-fetoprotein in a certain range
Quick detection, the successful building of the sensor provide platform for the detection of alpha-fetoprotein.
Summary of the invention
The object of the present invention is to provide a kind of alpha-fetoprotein optical electro-chemistry competitive immunoassay side based on Lazer's structure
Method and application.
To realize goal of the invention, the present invention adopts the following technical scheme: a kind of first tire based on Lazer's structure
Albumen optical electro-chemistry competitive immunoassay method, includes the following steps:
(1) pretreatment of glass-carbon electrode (GCE): GCE mechanical grinding first on the chamois leather for be covered with alumina powder polishes,
Remained on surface powder is washed away with secondary water, then moves into ultrasonic water bath and cleans, until cleaning up, finally sequentially uses ethyl alcohol, it is dilute
Acid and water thoroughly wash；
(2) Bi2S3The preparation of/IL/AFP modified electrode: the Bi for being 3mg/mL by 3 μ L concentration2S3Nanometer sheet is suspended in dry
Net glassy carbon electrode surface is dried under infrared lamp, is cooled to room temperature；It then is 1mg/ in above-mentioned 3 μ L concentration of electrode face finish
The ionic liquid (IL) of mL, is equally dried under infrared lamp, is cooled to room temperature to get Bi is arrived2S3/ IL modified electrode；Then, will
The electrode modified is immersed in containing 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride (EDC), N- hydroxysuccinimidyl
50min is activated in the mixed solution of acid imide (NHS)；Finally, it is 0.01ng/mL first tire that 7 μ L concentration are added dropwise on modified electrode
Albumen (AFP) (being purchased from Beijing BISS company) solution is simultaneously incubated for 50min, is allowed to be integrated to electrode by amido bond with EDC/NHS
Surface；Then extra AFP is removed with the phosphate buffer solution of pH7.0, then electrode is immersed into the BSA 1 that concentration is 1.0 wt.%
H, on enclosed-electrode surface nonspecific activity site, washes away after surface residual liquid to get to Bi2S3/ IL/AFP modified electrode；
(3) Bi2S3/IL/AFP/Ab-TiO2The preparation of-PEI modified electrode: firstly, being 3mg/mL's in 50 μ L concentration
TiO2The polyethyleneimine (PEI) that 5 μ L concentration are 10 mg/mL is added dropwise in mesomorphic and stirs 1 ~ 2 min, and freezes a few hours；It will
Mixed liquor is centrifuged 10 min and obtains sediment with 50 μ L milli-Q waters and dilute；Then, 10 μ L 5 are added in the solution
Wt.% glutaraldehyde is incubated for 1h at 4 DEG C；On this basis, by AFP monoclonal antibody (be purchased from Beijing BISS company) with it is above-mentioned molten
Liquid is that 1:4 ratio mixes and is incubated for 2h in refrigerator by volume；Finally, the mixture is centrifuged 10 min, supernatant is taken out,
Sediment is dissolved with 50 μ L ultrapure waters to get Ab-TiO is arrived2- PEI label probe；By AFP antigen (being purchased from Beijing BISS company)
Be configured to the standard solution of various concentration and with a certain number of Ab-TiO2The mixing of-PEI label probe；Finally by Bi2S3/IL/
AFP modified electrode immerses above-mentioned mixed solution, is incubated for 50min under 4 °C, makes Ab-TiO using competitive reaction2- PEI label is visited
Needle is incorporated in fixed electrode surface；Electrode surface residual solution and at room temperature oneself are rinsed with the phosphate buffer solution of pH7.0
It so dries to get Bi is arrived2S3/IL/AFP/Ab-TiO2- PEI modified electrode；(4) detection of alpha-fetoprotein: three-electrode system is used
It is measured, with GCE/Bi2S3/ IL/AFP modified electrode is working electrode, and Ag/AgCl is reference electrode, and platinum electrode is auxiliary
Electrode is detected using optical electro-chemistry work station, and setting voltage is 0.1V, carries out switch lamp, the list of xenon lamp transmitting every 10s
Coloured light excitation light source is filtered using preceding by monochromator；In the PBS buffer solution of pH 7.0, by optical electro-chemistry work station into
Row detection 1 × 10-6A series of alpha-fetoprotein standard solution of various concentrations between ng/mL -10 ng/mL is opened by record
The different current signals generated before and after turning off the light draw working curve；Testing sample solution is carried out instead of alpha-fetoprotein standard solution
Detection, the result of detection are checked in by working curve.
Above-mentioned Bi2S3The preparation of nanometer sheet:
By 0.00375 mol Bi (NO3)3· 5H2O(is purchased from Shenyang newly photochemical factory) it is dissolved with 25 mL ethyl alcohol, stirring
20 min obtained solution A；By 0.005625 mol Na2S is dissolved in 30 mL deionized waters, stirs 10 min, and B solution is made, will
Solution B is slowly added to solution A；When generating a large amount of black suspension objects, 0.032 mol urea (CO (NH is added2)2) and 20 mL go
Obtained mixed liquor is simultaneously sealed in autoclave by ionized water, keeps the temperature 12h under 180 °C；Then, filter mixed liquor obtains black
Solid product is washed with deionized water and dries in air；Finally by Bi obtained above2S3Nanometer sheet is dissolved in N, N- bis-
In methylformamide (DMF), and dilute the solution for being configured to various concentration.
Above-mentioned TiO2The preparation of mesomorphic material:
Firstly, 0.25g polyvinylpyrrolidone (PVP) is dissolved and is dispersed in 25mL HNO3In solution, 1g 12 is added
Sodium alkyl sulfate (SDS) stirs several minutes；Then, 0.5mL tetraisopropoxy titanium (TIP) is added in the above solution (purchased from south
Jing Pinning coupling agent Co., Ltd) and stir under 70 °C 50 h；Then, mixed liquor is centrifuged to obtain sediment, uses distilled water
It is thoroughly cleaned with ethyl alcohol, the dry diel at 60 DEG C；Finally, above-mentioned product to be calcined to about 120 min under 400 °C, to go
Except residual organic matter, pure TiO can be prepared by2Mesomorphic.
A kind of alpha-fetoprotein optical electro-chemistry based on Lazer's structure of the present invention competes immunosensor, including work
Make electrode, platinum electrode be to electrode and Ag/ AgCl is reference electrode, which is characterized in that the working electrode uses
Bi2S3/ IL/AFP/Ab modified electrode, 1 be prepared by the method for following step) glass-carbon electrode polishing: GCE exists first
It is covered with mechanical grinding on the chamois leather of alumina powder to polish, washes away remained on surface powder with secondary water, then move into ultrasonic water bath
Cleaning finally sequentially uses ethyl alcohol, diluted acid and water thoroughly wash until cleaning up；2) Bi2S3/IL/AFP/Ab-TiO2- PEI is repaired
Adorn the preparation of electrode: the Bi for being 3mg/mL by 3 μ L concentration2S3Nanometer sheet hanging drop is applied to clean glassy carbon electrode surface, red
It dries, is cooled to room temperature under outer lamp；Then in the ionic liquid (IL) that above-mentioned 3 μ L concentration of electrode face finish is 1mg/mL, together
Sample is dried under infrared lamp, is cooled to room temperature to get Bi is arrived2S3/ IL modified electrode；Then the electrode modified is immersed in and is contained
There are 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride (EDC), the mixing of n-hydroxysuccinimide (NHS) molten
50min is activated in liquid；Finally, it is 0.01ng/mL alpha-fetoprotein (AFP) (purchased from Beijing that 7 μ L concentration are added dropwise on above-mentioned electrode
BISS company) solution and it is incubated for 50min, it is allowed to be integrated to electrode surface by amido bond with EDC/NHS；Then with pH7.0's
Phosphate buffer solution removes extra AFP, then electrode is immersed concentration for 1 h of BSA of 1.0 wt.%, on enclosed-electrode surface
Nonspecific activity site is washed away after surface residual liquid to get to Bi2S3/ IL/AFP modified electrode；It is 3mg/ in 50 μ L concentration
The TiO of mL2The polyethyleneimine (PEI) that 5 μ L concentration are 10 mg/mL is added dropwise in mesomorphic and stirs 1 ~ 2 min, and it is small to freeze number
When；10 min of mixed liquor centrifugation are obtained into sediment with 50 μ L milli-Q waters and are diluted；Then, 10 μ L are added in the solution
5 wt.% glutaraldehydes are incubated for 1h at 4 DEG C；On this basis, by AFP monoclonal antibody (be purchased from Beijing BISS company) with it is above-mentioned
Solution is that 1:4 ratio mixes and is incubated for 2h in refrigerator by volume；Finally, the mixture is centrifuged 10 min, supernatant is taken out
Liquid arrives Ab-TiO with 50 μ L ultrapure waters dissolution sediment2- PEI label probe；AFP antigen is configured to various concentration
Standard solution and with a certain number of Ab-TiO2The mixing of-PEI label probe；Finally by Bi2S3On/IL/AFP modified electrode immerses
Mixed solution is stated, is incubated for 50min under 4 °C, makes Ab-TiO using competitive reaction2- PEI label probe is incorporated in fixed electrode table
Face；With the phosphate buffer solution of pH7.0 rinse electrode surface residual solution and at room temperature naturally dry to get to Bi2S3/
IL/AFP/Ab-TiO2- PEI modified electrode.
A kind of alpha-fetoprotein optical electro-chemistry competition immunologic pattern sensor based on Lazer's structure of the present invention is answered
With, which is characterized in that steps are as follows: 1) being measured using three-electrode system, with Bi2S3/IL/AFP/Ab-TiO2- PEI modification
Electrode is working electrode, and Ag/AgCl is reference electrode, and platinum electrode is auxiliary electrode, is examined using optical electro-chemistry work station
It surveys, setting voltage is 0.1V, carries out switch lamp every 10s, the monochromatic light excitation light source of xenon lamp transmitting is using preceding by monochromator mistake
Filter；2) in the PBS buffer solution of pH7.0, detection 1 × 10 is carried out by optical electro-chemistry work station-6Ng/mL -10ng/mL it
Between the alpha-fetoprotein standard solution of different solubility a series of drawn by the different current signals generated before and after record switch lamp
Working curve processed；Testing sample solution replaces alpha-fetoprotein standard solution to be detected, and the result of detection can pass through working curve
It checks in.
Remarkable advantage of the invention are as follows:
(1)TiO2Mesomorphic can fix more antibody as probe base, big specific surface area；High-crystallinity and orderly
Three-dimensional meso-hole structure be able to achieve the multiple reflections to light, to enhance the response of photoelectric current.
(2) electron-hole pair can not only be hindered by constructing a kind of Lazer's structure using two different optical active substances
It is compound, and expand light abstraction width and take full advantage of luminous energy, improve photoelectric current transfer efficiency to realize to photoelectricity
Stream signal be further amplified and the highly sensitive detection to alpha-fetoprotein.
(3) two kinds of light active materials are kept completely separate and pass through antigen in conjunction with the immune response between antibody by the sensor
To together, the construction process that this " signal is opened " type sensing modes simplify sensor also reduces the interference between different component.
Detailed description of the invention
Fig. 1 is the preparation process schematic diagram of the signal-on type optical electro-chemistry sensor of alpha-fetoprotein of the present invention.
Fig. 2 A is various concentration 1 × 10-6Ng/mL -10 ng/mL(a-h) alpha-fetoprotein standard solution, sensing electrode
Photocurrent response figure.
Fig. 2 B is the photocurrent response of sensing electrode and the linear relationship chart of alpha-fetoprotein concentration of standard solution.
Specific embodiment
The present invention is further illustrated by the following examples, but protection scope of the present invention is not limited to following reality
Apply example.
Embodiment 1
It is a kind of based on Lazer's structure alpha-fetoprotein optical electro-chemistry competition immunologic pattern sensor preparation (such as Fig. 1 institute
Show):
(1) pretreatment of glass-carbon electrode (GCE): GCE mechanical grinding first on the chamois leather for be covered with alumina powder is thrown
Light washes away remained on surface powder with secondary water, then moves into ultrasonic water bath and clean, until cleaning up, finally sequentially uses ethyl alcohol,
Diluted acid and water thoroughly wash；
(2) Bi that 3 μ L concentration are 3mg/mL is added dropwise2S3Nanometer sheet suspension is in clean glassy carbon electrode surface, infrared lamp
Lower drying, is cooled to room temperature；
(3) by 3 μ L concentration be 1mg/mL ionic liquid (IL) drop coating in above-mentioned electrode surface, dried under infrared lamp,
It is cooled to room temperature；
(4) electrode modified is immersed in containing 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride
(EDC), 50min is activated in the mixed solution of n-hydroxysuccinimide (NHS)；
(5) it is 0.01ng mL that 7 μ L concentration are added dropwise on above-mentioned electrode-1Alpha-fetoprotein (AFP) is (public purchased from Beijing BISS
Department) solution and it is incubated for 50min, it is allowed to be integrated to electrode surface by amido bond with EDC/NHS；
(6) extra AFP is removed with the phosphate buffer solution of pH 7.0, then it is 1.0 wt.%'s that electrode, which is immersed concentration,
1 h of BSA, on enclosed-electrode surface nonspecific activity site, washes away after surface residual liquid to get to Bi2S3/ IL/AFP modification
Electrode；
(7) in the TiO that 50 μ L concentration are 3mg/mL2The polyethyleneimine that 5 μ L concentration are 10 mg/mL is added dropwise in mesomorphic
(PEI) 1 ~ 2 min is stirred, and freezes a few hours；Mixed liquor is centrifuged 10 min and obtains sediment with 50 μ L milli-Q waters simultaneously
Dilution；Then, 10 μ L, 5 wt.% glutaraldehyde is added in the solution and is incubated for 1h at 4 DEG C；On this basis, by AFP monoclonal
Antibody (be purchased from Beijing BISS company) and above-mentioned solution are that 1:4 ratio mixes and is incubated for 2h in refrigerator by volume；Finally, will
The mixture is centrifuged 10 min, takes out supernatant, arrives Ab-TiO with 50 μ L ultrapure waters dissolution sediment2- PEI label is visited
Needle；By AFP antigen (be purchased from Beijing BISS company) be configured to various concentration standard solution and with a certain number of Ab-TiO2-
The mixing of PEI label probe；Finally by Bi2S3/ IL/AFP modified electrode immerses above-mentioned mixed solution, and 50min is incubated under 4 °C, benefit
Make Ab-TiO with competitive reaction2- PEI label probe is incorporated in fixed electrode surface；Electricity is rinsed with the phosphate buffer solution of pH7.0
Pole surface residual solution and at room temperature naturally dry to get arrive Bi2S3/IL/AFP/Ab-TiO2- PEI modified electrode.
Embodiment 2
A kind of application of the alpha-fetoprotein optical electro-chemistry competition immunologic pattern sensor based on Lazer's structure, steps are as follows:
(1) it is measured using three-electrode system, with Bi made from embodiment 12S3/IL/AFP/Ab-TiO2- PEI modification
Electrode is working electrode, and Ag/AgCl is reference electrode, and platinum electrode is to be detected to electrode using optical electro-chemistry work station,
Setting voltage is 0.1V, carries out switch lamp every 10s, the monochromatic light excitation light source of xenon lamp transmitting is filtered using preceding by monochromator；
(2) in the PBS buffer solution of pH 7.0, detection 1 × 10 is carried out by optical electro-chemistry work station-6 ng/mL–
A series of alpha-fetoprotein standard solution of various concentrations of 10 ng/mL is believed by the different electric currents generated before and after record switch lamp
Number, draw working curve.Fig. 2 A is various concentration 1 × 10-6Ng/mL -10ng/mL(a-h) alpha-fetoprotein standard solution, sensing
The photocurrent response of electrode.Fig. 2 B is the photocurrent response of sensing electrode and the linear relationship of alpha-fetoprotein concentration of standard solution
Figure.
(3) alpha-fetoprotein standard solution is replaced to detect testing sample solution, the result of detection can pass through work song
Line checks in.
Embodiment 3
Bi described in embodiment 12S3The preparation of nanometer sheet:
By 0.00375 mol Bi (NO3)3· 5H2O(is purchased from Shenyang newly photochemical factory) it is dissolved with 25 mL ethyl alcohol, stirring
20 min obtained solution A；By 0.005625 mol Na2S is dissolved in 30 mL deionized waters, stirs 10 min, and B solution is made, will
Solution B is slowly added to solution A；When generating a large amount of black suspension objects, 0.032 mol urea (CO (NH is added2)2) and 20 mL go
Obtained mixed liquor is simultaneously sealed in autoclave by ionized water, keeps the temperature 12h under 180 °C；Then, filter mixed liquor obtains black
Solid product is washed with deionized water and dries in air；Finally by Bi obtained above2S3Nanometer sheet is dissolved in N, N- bis-
In methylformamide (DMF), and dilute the solution for being configured to various concentration.
TiO described in embodiment 12The preparation of mesomorphic:
Firstly, 0.25g polyvinylpyrrolidone (PVP) is dissolved and is dispersed in 25mL HNO3In solution, 1g 12 is added
Sodium alkyl sulfate (SDS) stirs several minutes；Then, 0.5mL tetraisopropoxy titanium (TIP) is added in the above solution (purchased from south
Jing Pinning coupling agent Co., Ltd) and stir under 70 °C 50 h；Then, mixed liquor is centrifuged to obtain sediment, uses distilled water
It is thoroughly cleaned with ethyl alcohol, the dry diel at 60 DEG C；Finally, above-mentioned product to be calcined to about 120 min under 400 °C, to go
Except residual organic matter, pure TiO can be prepared by2Mesomorphic.
Claims (4)
1. a kind of preparation method of the alpha-fetoprotein optical electro-chemistry competition immunologic pattern sensor based on Lazer's structure, feature exist
In, comprising the following steps:
(1) pretreatment of glass-carbon electrode: glass-carbon electrode mechanical grinding first on the chamois leather for be covered with alumina powder polishes, with two
Remained on surface powder is removed in secondary washing, then moves into ultrasonic water bath and clean, until clean up, finally sequentially uses ethyl alcohol, diluted acid and
Water thoroughly washs；
(2) Bi2S3The preparation of/IL/AFP modified electrode: the Bi for being 3mg/mL by 3 μ L concentration2S3Nanometer sheet hanging drop is applied to
Clean glassy carbon electrode surface is dried under infrared lamp, is cooled to room temperature；Then it is in above-mentioned 3 μ L concentration of electrode face finish
The ionic liquid (IL) of 1mg/mL, Drying and cooling arrives Bi to room temperature under infrared lamp2S3/ IL modified electrode；Then, it will repair
The electrode adornd is immersed in containing 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride (EDC), N- hydroxysuccinimidyl acyl
50min is activated in the mixed solution of imines (NHS)；Finally, it is 0.01ng/mL first tire egg that 7 μ L concentration are added dropwise on modified electrode
White (AFP) solution is simultaneously incubated for 50min, is allowed to and 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride/N- hydroxyl
Succinimide is integrated to electrode surface by amido bond；Then extra AFP is removed with the phosphate buffer solution of pH7.0, then
Electrode is immersed into 1 h of BSA that concentration is 1.0 wt.%, surface residual is washed away in nonspecific activity site on enclosed-electrode surface
After liquid to get arrive Bi2S3/ IL/AFP modified electrode；
(3) Bi2S3/IL/AFP/Ab-TiO2The preparation of-PEI modified electrode: firstly, in the TiO that 50 μ L concentration are 3mg/mL2It is situated between
The polyethyleneimine (PEI) that 5 μ L concentration are 10 mg/mL is added dropwise in crystalline substance and stirs 1 ~ 2 min, and freezes a few hours；By mixed liquor
10 min are centrifuged to obtain sediment with 50 μ L milli-Q waters and dilute；Then, 10 μ L, 5 wt.% penta 2 is added in the solution
Aldehyde is incubated for 1h at 4 DEG C；On this basis, by AFP monoclonal antibody and above-mentioned solution be by volume 1:4 ratio mix and
2h is incubated in refrigerator；Finally, by the mixture be centrifuged 10 min, take out supernatant, with 50 μ L ultrapure waters dissolution sediment to get
To Ab-TiO2- PEI label probe；By AFP antigen be configured to various concentration standard solution and with a certain number of Ab-TiO2-
The mixing of PEI label probe；Finally by Bi2S3/ IL/AFP modified electrode immerses above-mentioned mixed solution, and 50min is incubated under 4 °C, benefit
Make Ab-TiO with competitive reaction2- PEI label probe is incorporated in fixed electrode surface；Electricity is rinsed with the phosphate buffer solution of pH7.0
Pole surface residual solution and at room temperature naturally dry to get arrive Bi2S3/IL/AFP/Ab-TiO2- PEI modified electrode.
2. the method according to claim 1, wherein the Bi2S3Nanometer sheet is prepared by the following method: will
0.00375 mol Bi(NO3)3· 5H2O is dissolved with 25 mL ethyl alcohol, stirs 20 min obtained solution A；By 0.005625 mol
Na2S is dissolved in 30 mL deionized waters, stirs 10 min, and solution B is slowly added to solution A by obtained solution B；It is a large amount of black when generating
When color suspended matter, 0.032 mol urea (CO (NH is added2)2) and 20 mL deionized waters and be sealed in obtained mixed liquor
In autoclave, 12h is kept the temperature under 180 °C；Then, filter mixed liquor obtains black solid product, is washed with deionized water and in sky
It is dry in gas；Finally by Bi obtained above2S3Nanometer sheet is dissolved in n,N-Dimethylformamide (DMF), and dilutes preparation
At the solution of required concentration.
3. the method according to claim 1, wherein the TiO2Mesomorphic is prepared by the following method: firstly, will
0.25g polyvinylpyrrolidone (PVP) dissolves and is dispersed in 25mL HNO3In solution, 1g lauryl sodium sulfate is added
(SDS), it stirs several minutes；Then, 0.5mL tetraisopropoxy titanium (TIP) is added in the above solution and stirs 50 under 70 °C
h；Then, mixed liquor is centrifuged to obtain sediment, is thoroughly cleaned with distilled water and ethyl alcohol, the dry diel at 60 DEG C；Finally,
Above-mentioned product is calcined to about 120 min under 400 °C, to remove residual organic matter, can be prepared by pure TiO2Mesomorphic.
4. a kind of alpha-fetoprotein optical electro-chemistry based on Lazer's structure competes immunosensor, including working electrode, platinum filament electricity
It is extremely reference electrode to electrode and Ag/ AgCl, which is characterized in that the working electrode uses Bi2S3/IL/AFP/Ab-
TiO2- PEI modified electrode, the Bi2S3/IL/AFP/Ab-TiO2Made of-PEI modified electrode is prepared by the following method: 1)
The polishing of glass-carbon electrode: glass-carbon electrode mechanical grinding first on the chamois leather for be covered with alumina powder polishes, and is washed away with secondary water
Remained on surface powder, then move into ultrasonic water bath and clean, until cleaning up, ethyl alcohol is finally sequentially used, diluted acid and water are thoroughly washed
It washs；2) Bi2S3/IL/AFP/Ab-TiO2The preparation of-PEI modified electrode: the Bi for being 3mg/mL by 3 μ L concentration2S3Nanometer sheet suspends
It dries, is cooled to room temperature under clean glassy carbon electrode surface, infrared lamp；Then it is in above-mentioned 3 μ L concentration of electrode face finish
The ionic liquid (IL) of 1mg/mL, is equally dried under infrared lamp, is cooled to room temperature to get Bi is arrived2S3/ IL modified electrode；With
Afterwards, the electrode modified is immersed in containing 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride (EDC), N- hydroxyl
50min is activated in the mixed solution of base succinimide (NHS)；Finally, it is 0.01ng/ that 7 μ L concentration are added dropwise on modified electrode
ML alpha-fetoprotein (AFP) solution is simultaneously incubated for 50min, be allowed to 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride/
N-hydroxysuccinimide is integrated to electrode surface by amido bond；Then it is extra to be removed with the phosphate buffer solution of pH7.0
AFP, then electrode is immersed into 1 h of BSA that concentration is 1.0 wt.%, table is washed away in nonspecific activity site on enclosed-electrode surface
After the residual solution of face to get arrive Bi2S3/ IL/AFP modified electrode；In the TiO that 50 μ L concentration are 3mg/mL25 μ L are added dropwise in mesomorphic
The polyethyleneimine (PEI) that concentration is 10 mg/mL stirs 1 ~ 2 min, and freezes a few hours；Mixed liquor is centrifuged 10 min to obtain
It with 50 μ L milli-Q waters and is diluted to sediment；Then, 10 μ L, 5 wt.% glutaraldehyde is added in the solution to be incubated at 4 DEG C
1h；On this basis, AFP monoclonal antibody and above-mentioned solution are the mixing of 1:4 ratio by volume and are incubated for 2h in refrigerator；
Finally, the mixture is centrifuged 10 min, supernatant is taken out, arrives Ab-TiO with 50 μ L ultrapure waters dissolution sediment2-PEI
Label probe；By AFP antigen be configured to various concentration standard solution and with a certain number of Ab-TiO2- PEI label probe is mixed
It closes；Finally by Bi2S3/ IL/AFP modified electrode immerses above-mentioned mixed solution, is incubated for 50min under 4 °C, is made using competitive reaction
Ab-TiO2- PEI label probe is incorporated in fixed electrode surface；Electrode surface residual solution is rinsed with the phosphate buffer solution of pH7.0
And at room temperature naturally dry to get arrive Bi2S3/IL/AFP/Ab-TiO2- PEI modified electrode.
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| CN110244061B (en) * | 2019-07-17 | 2022-09-27 | 福建师范大学 | Zearalenone multichannel signal detection immunoassay method based on spiral carbon nanotube photothermal effect |
| CN110308286B (en) * | 2019-07-24 | 2022-08-30 | 福建师范大学 | Enhanced thyroglobulin electrochemiluminescence immunosensor based on photothermal release signals |
| CN110361533A (en) * | 2019-08-02 | 2019-10-22 | 福建师范大学 | A kind of interlayer type prostate-specific antigen optical electro-chemistry immunoassay method based on signals in situ amplification |
| CN111796009A (en) * | 2020-07-20 | 2020-10-20 | 济南大学 | Design and synthesis of triphenylamine probe for formaldehyde selective photoelectrochemical sensing |
| CN112964875B (en) * | 2021-02-26 | 2022-08-30 | 福建师范大学 | Human papillomavirus 16 type E6 protein multi-mode immunoassay method based on multifunctional clinical vaginal swab |
| CN113514522A (en) * | 2021-04-26 | 2021-10-19 | 济南大学 | Novel dye-sensitized semiconductor composite material electrode |
| CN114137217A (en) * | 2021-11-30 | 2022-03-04 | 中国科学院长春光学精密机械与物理研究所 | FRET-based homogeneous immunoassay method and detection composition |
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