CN110308286A - One kind being based on the enhanced thyroglobulin electrochemiluminescimmunosensor immunosensor of photo-thermal release signal - Google Patents
One kind being based on the enhanced thyroglobulin electrochemiluminescimmunosensor immunosensor of photo-thermal release signal Download PDFInfo
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- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
Abstract
The present invention discloses a kind of based on the enhanced thyroglobulin electrochemiluminescimmunosensor immunosensor of photo-thermal release signal.The construction method of the sensor is fixed thyroglobulin antibody using silica-based titanium dioxide magnetic bead as substrate, mesoporous silicon oxide to adsorb bipyridyl ruthenium marks thyroglobulin as signal probe, constructs a kind of competitive type immunosensor by specific recognition between antigen and antibody.The electrode for modifying antibody is put into the mixed solution containing various concentration free antigen and a certain amount of signal probe, free target antigen and signal probe competitive binding antibody are made, the electrode for combining signal probe can generate certain luminous intensity.Under the irradiation of 808nm infrared laser, sensing interface is brought rapidly up, and the bipyridyl ruthenium release of mesoporous silicon oxide absorption, luminous signal is significantly increased with temperature raising, to realize signal amplification.And as the increase of free target concentration, the quantity of antibody binding signal probe are reduced, luminous intensity also reduces therewith, can set up the Electrochemiluminescprocess process method to thyroglobulin based on the phenomenon.
Description
Technical field
The invention belongs to new function materials and bio-sensing detection technique field, and in particular to one kind is discharged based on photo-thermal
Detection method of the signal enhancing type electrochemiluminescimmunosensor immunosensor to thyroglobulin.
Background technique
Electrochemiluminescimmunosensor immunosensor combines with immunoassay electrogenerated chemiluminescence method (ECL) available
In clinical diagnosis, have many advantages, such as that high sensitivity, high specificity, selectivity are good, easy to operate.It is introduced into ECL system anti-altogether
It answers agent that can significantly increase its luminous signal intensity, effectively improves the sensitivity of immunosensor.Present invention firstly provides tetrems
Five amine of alkene significantly enhances ECL in conjunction with mesoporous silicon oxide photo-thermal release action as the novel coreaction reagent of bipyridyl ruthenium
Signal realizes the highly sensitive detection to thyroid cancer marker.
Tetraethylenepentamine (TEPA) is a kind of fatty amine, has that toxicity is low, good biocompatibility, to be easy to immobilization etc. excellent
Point.Bipyridyl ruthenium Ru (bpy)3 2+Classical coreaction reagent such as tripropyl amine (TPA) (TPA), the amino that surface is not dissociated, thus nothing
The direct functionalization immune complex of method greatly limits the application that TPA shines in ECL solid phase.Compared with TPA, the trip of the both ends TEPA
From amino be easily functionalized, as bipyridyl ruthenium coreaction reagent have and the comparable ECL humidification of TPA.Currently, base
It is widely used in the signal amplification strategy of mesoporous material in electrogenerated chemiluminescence sensory field.Mesoporous silicon oxide
(MPS) there is unique meso-hole structure and excellent performance, Ru (bpy)3 2+The compound (Ru@MPS) of MPS is adulterated as immune
The ideal mark of analysis, because its easy preparation and good dissolubility, excellent specific surface area are conducive to adsorb a large amount of Ru
(bpy)3 2+, can be effectively conjugated with immune molecule and promote ECL signal.In addition, silica-based titanium dioxide magnetic bead (MB) has magnetism
As base material, the binding force with sensing interface can be enhanced, and enhance ECL signal.Under 808 nm laser irradiations, building
The enhanced electrochemiluminescimmunosensor immunosensor of photo-thermal release signal, highly sensitive inspection for thyroid cancer marker TG
It surveys.Specifically, the bipyridyl ruthenium of mesoporous silicon oxide absorption discharges under light and heat condition, and burst size is significant because temperature increases to be increased
Add, to enhance ECL signal, and in a linear relationship with TG concentration in a certain range.This electricity enhanced based on photo-thermal
It causes chemiluminescence immunoassay sensor to improve the sensitivity and accuracy of detection, there is very important answer in terms of clinical diagnosis
With value and practical significance.
Summary of the invention
The object of the present invention is to provide one kind based on the enhanced electrochemiluminescimmunosensor immunosensor of photo-thermal release signal and
Preparation method and detection method to thyroglobulin.
To realize goal of the invention, the present invention adopts the following technical scheme:
(1) pretreatment of GCE: GCE mechanical grinding first on the chamois leather for be covered with alumina powder polishes, and washes away table with secondary water
Face residual powder, then move into ultrasonic water bath and clean, until cleaning up, ethyl alcohol is finally sequentially used, diluted acid and water thoroughly wash；
(2) MPS-Ru (bpy)3 2+The preparation of/TEPA/TG/Ab/MB modified electrode: magnetic bead (MB) powder of 5 mg is dissolved in 1 mL
Then 20 wt.% ethyl alcohol take 3 μ L MB solution drop coatings in clean glassy carbon electrode surface, dry in an oven, be cooled to room
Temperature, then take the 3 water-soluble drop-coateds of μ L 1mM mercaptoethylmaine on the electrode, 4 °C of lower 40 min of functionalization；The 10 μ L concentration ratios are taken to be
4:1's contains 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride (EDC), n-hydroxysuccinimide (NHS)
Mixed solution is added in 1ng/mL thyroglobulin antibody (Ab) solution, and 40 min are activated at 4 DEG C；By above-mentioned modification electricity
Pole is immersed in the Ab solution after activation, and 50 min are incubated under 4 °C；Then removed with the phosphate buffer solution (PBS) of pH 7.4
Electrode is finally immersed 1 h of BSA aqueous solution that concentration is 1.0 wt.% by extra Ab, and non-specificity is living on enclosed-electrode surface
Property site, wash away after surface residual liquid to get to Ab/MB modified electrode；TG is configured to the standard solution difference of various concentration
With MPS-Ru (bpy)3 2+/ TEPA/TG label probe solution is mixed to form mixed solution, and the immersion of Ab/MB modified electrode is above-mentioned
Mixed solution is incubated for 50 min under 4 °C, makes MPS-Ru (bpy) by competitive reaction3 2+/ TEPA/TG label probe is incorporated in
Modified electrode surface；With the PBS of pH 7.4 rinse electrode surface residual solution and at room temperature naturally dry to get to MPS-
Ru(bpy)3 2+/ TEPA/TG/Ab/MB modified electrode；
(detection of 3 thyroglobulins: being measured using three-electrode system, with MPS-Ru (bpy)3 2+/TEPA/TG/Ab/MB
Modified electrode is working electrode, and Ag/AgCl is reference electrode, and platinum electrode is auxiliary electrode, to first on electrochemical workstation
Shape gland globulin is detected, and electrochemical workstation is connected with chemiluminescence detector and the high pressure of photomultiplier tube is arranged
For 700 V, cyclic voltammetry scan potential range is 0 ~ 1.6 V, 0.15 V/s of sweep speed；In the PBS of pH 8.0, pass through
Electrogenerated chemiluminescence method, to 1 × 10-1 μg/mL~1×10-7A series of thyroglobulin standard of various concentrations of μ g/mL
The electrochemiluminescence signal intensity that solution generates on modified electrode is measured, and draws working curve；Sample to be tested is molten
Liquid replaces thyroglobulin standard solution to be detected, and the result of detection is checked in by working curve.
The preparation of above-mentioned mesoporous silicon oxide (MPS):
It weighs 0.5 g cetyl trimethylammonium bromide (CTAB) to be dissolved in 240 mL ultrapure waters, 3.5 mL, 2.0 M hydrogen is added
Sodium hydroxide solution, be sufficiently stirred it is lower mixed solution is warming up to 80 °C, the positive silicon of 2.5 mL is slowly added dropwise in 30 min of insulation reaction
Acetoacetic ester (TEOS) then continues to be stirred to react 2 h under 80 °C, which is centrifuged 10 min, gained is precipitated with ultrapure
Water and ethyl alcohol respectively wash three times, and white solid is obtained after vacuum drying；Finally, 0.5 g obtained solid is taken to be added to 50 mL methanol
In the concentrated hydrochloric acid mixed solution of 2.5 mL, 37 wt %, 16 h are heated to reflux, filtering gained precipitating uses ultrapure water and first respectively
Alcohol washs three times, and under 60 °C of vacuum in dry removal duct remaining solvent to get arriving mesoporous silicon oxide；
Mesoporous silicon oxide adsorbs bipyridyl ruthenium [MPS-Ru (bpy)3 2+] preparation:
Firstly, by the bipyridyl ruthenium [Ru of 10 mM of mesoporous silicon oxide (MPS) aqueous solution and 50 μ L of 50 μ L, 10 mg/mL
(bpy)3 2+] solution shakes up 6 h of absorption, mixed liquor is centrifuged 10 min and obtains sediment with 100 μ L milli-Q waters and dilutes；
Then, it is added 10 μ L 3- aminopropyl triethoxysilane (APTES) functionalization, 12 h in the solution, and with milli-Q water,
It is added at 4 DEG C of 10 μ L, 5 wt.% glutaraldehyde in the solution and is crosslinked 1 h；
Mesoporous silicon oxide adsorbs bipyridyl ruthenium labelled antigen [MPS-Ru (bpy)3 2+/ TEPA/TG] preparation:
Take the above-mentioned [MPS-Ru (bpy) of 50 μ L3 2+] solution addition 30 μ L, 1 ng/mL thyroglobulin standard solution (TG)
In the mixed solution of 20 μ L, 1 mM tetraethylenepentamine (TEPA), 50 min are reacted at 4 DEG C, and with the BSA of 1.0 wt.%
Aqueous solution closing；Finally, the mixture is centrifuged 10 min, MPS-Ru is arrived with the ultrapure water dispersion sediment of 50 μ L
(bpy)3 2+/ TEPA/TG label probe solution is dispersed with the PBS of pH 7.4, adds 20 μ L BSA aqueous solutions, places 4 °C
Under the conditions of it is spare.
A kind of electrochemiluminescimmunosensor immunosensor enhanced based on photo-thermal release signal of the present invention, including work
Make electrode, platinum electrode be to electrode and Ag/AgCl is reference electrode, which is characterized in that the working electrode uses MPS-
Ru(bpy)3 2+/ TEPA/TG/Ab/MB modified electrode, the MPS-Ru (bpy)3 2+/ TEPA/TG/Ab/MB modified electrode is under
What the method for stating was prepared: 1) polishing of glass-carbon electrode: glass-carbon electrode is mechanical first on the chamois leather for be covered with alumina powder to be beaten
Grinding and polishing light washes away remained on surface powder with secondary water, then moves into ultrasonic water bath and clean, until cleaning up, finally sequentially uses
Ethyl alcohol, diluted acid and water thoroughly wash；2) MPS-Ru (bpy)3 2+The preparation of/TEPA/TG/Ab/MB modified electrode: by the magnetic of 5 mg
Pearl (MB) powder is dissolved in 1 mL, 20 wt.% ethyl alcohol, then, takes 3 μ L MB solution drop coatings in clean glassy carbon electrode surface,
It dries, is cooled to room temperature in baking oven, then take the 3 water-soluble drop-coateds of μ L 1mM mercaptoethylmaine on the electrode, 4 °C of lower functionalization 40
min；Take 10 μ L concentration than containing 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride (EDC), N- hydroxyl for 4:1
The mixed solution of base succinimide (NHS) is added in 1ng/mL thyroglobulin antibody (Ab) solution, is activated at 4 DEG C
40 min；Above-mentioned modified electrode is immersed in the Ab solution after activation, 50 min are incubated under 4 °C；Then use the phosphorus of pH 7.4
Acid buffering solution (PBS) removes extra Ab, and electrode is finally immersed 1 h of BSA aqueous solution that concentration is 1.0 wt.%, closing electricity
Nonspecific activity site in pole surface is washed away after surface residual liquid to get to Ab/MB modified electrode；TG is configured to difference
The standard solution of concentration respectively with MPS-Ru (bpy)3 2+/ TEPA/TG label probe solution is mixed to form mixed solution, and by Ab/
MB modified electrode immerses above-mentioned mixed solution, and 50 min are incubated under 4 °C, make MPS-Ru (bpy) by competitive reaction3 2+/
TEPA/TG label probe is incorporated in modified electrode surface；Electrode surface residual solution is rinsed with the PBS of pH 7.4 and in room temperature condition
Lower naturally dry to get arrive MPS-Ru (bpy)3 2+/ TEPA/TG/Ab/MB modified electrode.
A kind of enhanced electrochemiluminescimmunosensor immunosensor of photo-thermal release signal that is based on of the present invention is to thyroid gland
The detection method of globulin, which is characterized in that steps are as follows: 1) being measured using three-electrode system, with MPS-Ru
(bpy)3 2+/ TEPA/TG/Ab/MB modified electrode is working electrode, and Ag/AgCl is reference electrode, and platinum electrode is auxiliary electrode,
Thyroglobulin is detected on electrochemical workstation, electrochemical workstation is connected and incited somebody to action with chemiluminescence detector
The high pressure of photomultiplier tube is set as 700 V, and cyclic voltammetry scan potential range is 0 ~ 1.6 V, 0.15 V/s of sweep speed；
2) in the PBS of pH 8.0, by electrogenerated chemiluminescence method, to 1 × 10-1 μg/mL~1×10-7A series of differences of μ g/mL
The electrochemiluminescence signal intensity that the thyroglobulin standard solution of concentration generates on modified electrode is measured, and is drawn
Working curve；Thyroglobulin standard solution is replaced to detect testing sample solution, the result of detection passes through work song
Line checks in.
Remarkable advantage of the invention are as follows:
(1) it has been put forward for the first time novel coreaction reagent of the tetraethylenepentamine as bipyridyl ruthenium, has significantly enhanced ECL signal, it is real
The highly sensitive detection to thyroid cancer marker is showed.
(2) MPS has unique meso-hole structure and excellent performance, Ru (bpy)3 2+Adulterate the compound (Ru@MPS) of MPS
As the ideal mark of immunoassay, because its easy preparation and good dissolubility, excellent specific surface area are conducive to adsorb
A large amount of Ru (bpy)3 2+, can be effectively conjugated with immune molecule and promote ECL signal.
(3) under the irradiation of 808nm infrared laser, sensing interface is brought rapidly up, the connection pyrrole of mesoporous silicon oxide absorption
The release of pyridine ruthenium, luminous signal are significantly increased with temperature raising, to realize signal amplification.
Detailed description of the invention
Fig. 1 is that mesoporous silicon oxide adsorbs bipyridyl ruthenium (a) and (b) and mesoporous silicon oxide before illumination after illumination
(c) ultraviolet-visible spectrogram.
Fig. 2 A is immune sensing electrode in various concentration 1 × 10-1 μg/mL~1×10-7μ g/mL(a-g) thyroid gland ball
The electrogenerated chemiluminescence response diagram of protein standard solution.
Fig. 2 B responds linear with thyroglobulin concentration of standard solution for the electrogenerated chemiluminescence of immune sensing electrode
Relational graph.
Specific embodiment
The present invention is further illustrated by the following examples, but protection scope of the present invention is not limited to following reality
Apply example.
Embodiment 1
A kind of preparation method based on the enhanced electrochemiluminescimmunosensor immunosensor of photo-thermal release signal:
(1) pretreatment of glass-carbon electrode: glass-carbon electrode mechanical grinding first on the chamois leather for be covered with alumina powder polishes, with two
Remained on surface powder is removed in secondary washing, then moves into ultrasonic water bath and clean, until clean up, finally sequentially uses ethyl alcohol, diluted acid and
Water thoroughly washs；
(2) by the bipyridyl ruthenium [Ru of 10 mM of mesoporous silicon oxide (MPS) aqueous solution and 50 μ L of 50 μ L, 10 mg/mL
(bpy)3 2+] solution shakes up 6 h of absorption, mixed liquor is centrifuged 10 min and obtains sediment with 100 μ L milli-Q waters and dilutes；
Then, it is added 10 μ L 3- aminopropyl triethoxysilane (APTES) functionalization, 12 h in the solution, and with milli-Q water,
It is added at 4 DEG C of 10 μ L, 5 wt.% glutaraldehyde in the solution and is crosslinked 1 h, mesoporous silicon oxide absorption bipyridyl ruthenium can be obtained
[MPS-Ru(bpy)3 2+]；
(3) above-mentioned [MPS-Ru (bpy) of 50 μ L is taken3 2+] solution addition 30 μ L, 1 ng/mL thyroglobulin standard solution
(TG) and in the mixed solution of 20 μ L, 1 mM tetraethylenepentamine (TEPA), 50 min are reacted at 4 DEG C, and with 1.0 wt.%
BSA aqueous solution closing；Finally, the mixture is centrifuged 10 min, MPS- is arrived with the ultrapure water dispersion sediment of 50 μ L
Ru(bpy)3 2+/ TEPA/TG label probe solution is dispersed with the PBS of pH 7.4, adds 20 μ L BSA aqueous solutions, places 4 °
It is spare under the conditions of C.
(4) magnetic bead (MB) powder of 5 mg is dissolved in 1 mL, 20 wt.% ethyl alcohol, then, take 3 μ L MB solution drop coatings in
Clean glassy carbon electrode surface, is dried in an oven, is cooled to room temperature, then takes the 3 water-soluble drop-coateds of μ L 1mM mercaptoethylmaine in electricity
On extremely, 4 °C of lower 40 min of functionalization；Take 10 μ L concentration than containing 1- (3- dimethylamino-propyl) -3- ethyl carbon for 4:1
Diimmonium salt hydrochlorate (EDC), n-hydroxysuccinimide (NHS) mixed solution be added to 1ng/mL thyroglobulin antibody
(Ab) in solution, 40 min are activated at 4 DEG C；Above-mentioned modified electrode is immersed in the Ab solution after activation, is incubated under 4 °C
50 min then remove extra Ab with the phosphate buffer solution (PBS) of pH 7.4, and it is 1.0 that electrode, which is finally immersed concentration,
1 h of BSA aqueous solution of wt.%, on enclosed-electrode surface nonspecific activity site, washes away after surface residual liquid to get to Ab/
MB modified electrode；
(5) by thyroglobulin antibody (TG) be configured to the standard solution of various concentration respectively with MPS- made from step (3)
Ru(bpy)3 2+The mixing of/TEPA/TG label probe solution, and Ab/MB modified electrode made from step (4) is immersed into above-mentioned mixing
Solution is incubated for 50 min under 4 °C, makes MPS-Ru (bpy) by competitive reaction3 2+/ TEPA/TG label probe is incorporated in modification
Electrode surface；With the PBS of pH 7.4 rinse electrode surface residual solution and at room temperature naturally dry to get to MPS-Ru
(bpy)3 2+/ TEPA/TG/Ab/MB modified electrode；
Above-mentioned magnetic bead (MB) used is silica-based titanium dioxide magnetic bead, is bought from Xiamen Pu Ruimai lattice Biotechnology Co., Ltd,
Tetraethylenepentamine (TEPA) is bought from Aladdin reagent (Shanghai) Co., Ltd., thyroglobulin antibody (Ab) and thyroid gland ball
Protein antibodies (TG) are purchased from the silent picogram Biotechnology Co., Ltd in Wuhan.
Embodiment 2
The preparation of mesoporous silicon oxide used in embodiment 1 (MPS): 0.5 g cetyl trimethylammonium bromide (CTAB) is weighed
It is dissolved in 240 mL ultrapure waters, 3.5 mL, 2.0 M sodium hydroxide solution is added, be sufficiently stirred and lower mixed solution be warming up to 80
°C, 30 min of insulation reaction is slowly added dropwise 2.5 mL ethyl orthosilicates (TEOS), then continues to be stirred to react 2 under 80 °C
The mixture is centrifuged 10 min by h, and gained precipitating ultrapure water and ethyl alcohol respectively wash three times, and it is solid that white is obtained after vacuum drying
Body；Finally, 0.5 g obtained solid is taken to be added in the concentrated hydrochloric acid mixed solution of 50 mL methanol and 2.5 mL 37wt. %, heat
Flow back 16 h, and filtering gained precipitating is washed three times with ultrapure water and methanol respectively, and under 60 °C of vacuum in dry removal duct
Remaining solvent to get arrive mesoporous silicon oxide.
Above-mentioned cetyl trimethylammonium bromide (CTAB) used, ethyl orthosilicate (TEOS) are bought from Chinese medicines group
Learn reagent Co., Ltd.
Embodiment 3
One kind is based on the enhanced thyroglobulin electrochemiluminescimmunosensor immunosensor of photo-thermal release signal to thyroid gland ball egg
White to be detected, steps are as follows:
(1) it is measured using three-electrode system, with MPS-Ru made from embodiment 1 (bpy)3 2+/ TEPA/TG/Ab/MB modification
Electrode is working electrode, and Ag/AgCl is reference electrode, and platinum electrode is auxiliary electrode, to thyroid gland on electrochemical workstation
Globulin is detected, and electrochemical workstation is connected with chemiluminescence detector and sets the high pressure of photomultiplier tube to
700 V, cyclic voltammetry scan potential range are 0 ~ 1.6 V, 0.15 V/s of sweep speed；(2) in the PBS of pH 8.0, pass through
Electrogenerated chemiluminescence method, to 1 × 10-1 μg/mL~1×10-7A series of thyroglobulin standard of various concentrations of μ g/mL
The electrochemiluminescence signal intensity that solution generates on modified electrode is measured, and draws working curve；
(3) thyroglobulin standard solution is replaced to detect testing sample solution, the result of detection passes through working curve
It checks in.
Claims (5)
1. a kind of preparation method based on the enhanced thyroglobulin electrochemiluminescimmunosensor immunosensor of photo-thermal release signal,
Characterized by comprising the following steps:
(1) pretreatment of glass-carbon electrode (GCE): glass-carbon electrode mechanical grinding first on the chamois leather for be covered with alumina powder is thrown
Light washes away remained on surface powder with secondary water, then moves into ultrasonic water bath and clean, until cleaning up, finally sequentially uses ethyl alcohol,
Diluted acid and water thoroughly wash；
(2) MPS-Ru (bpy)3 2+The preparation of/TEPA/TG/Ab/MB modified electrode: magnetic bead (MB) powder of 5 mg is dissolved in 1 mL
Then 20 wt.% ethyl alcohol take 3 μ L MB solution drop coatings in clean glassy carbon electrode surface, dry in an oven, be cooled to room
Temperature, then take the 3 water-soluble drop-coateds of μ L 1mM mercaptoethylmaine on the electrode, 4 °C of lower 40 min of functionalization；The 10 μ L concentration ratios are taken to be
4:1's contains 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride (EDC), n-hydroxysuccinimide (NHS)
Mixed solution is added in 1ng/mL thyroglobulin antibody (Ab) solution, and 40 min are activated at 4 DEG C；By above-mentioned modification electricity
Pole is immersed in the Ab solution after activation, and 50 min are incubated under 4 °C；Then removed with the phosphate buffer solution (PBS) of pH 7.4
Electrode is finally immersed 1 h of BSA aqueous solution that concentration is 1.0 wt.% by extra Ab, and non-specificity is living on enclosed-electrode surface
Property site, wash away after surface residual liquid to get to Ab/MB modified electrode；Thyroglobulin standard solution (TG) is configured to
The standard solution of various concentration respectively with MPS-Ru (bpy)3 2+/ TEPA/TG label probe solution is mixed to form mixed solution, and
Ab/MB modified electrode is immersed into above-mentioned mixed solution, 50 min are incubated under 4 °C, MPS-Ru is made by competitive reaction
(bpy)3 2+/ TEPA/TG label probe is incorporated in modified electrode surface；With the PBS of pH 7.4 rinse electrode surface residual solution and
Under room temperature naturally dry to get arrive MPS-Ru (bpy)3 2+/ TEPA/TG/Ab/MB modified electrode；
(4) detection of thyroglobulin: being measured using three-electrode system, with MPS-Ru (bpy)3 2+/TEPA/TG/Ab/
MB modified electrode is working electrode, and Ag/AgCl is reference electrode, and platinum electrode is auxiliary electrode, right on electrochemical workstation
Thyroglobulin is detected, and electrochemical workstation is connected with chemiluminescence detector and sets the high pressure of photomultiplier tube
700 V are set to, cyclic voltammetry scan potential range is 0 ~ 1.6 V, 0.15 V/s of sweep speed；In the PBS of pH 8.0, lead to
Electrogenerated chemiluminescence method is crossed, to 1 × 10-1 μg/mL~1×10-7A series of thyroglobulin mark of various concentrations of μ g/mL
The electrochemiluminescence signal intensity that quasi- solution generates on modified electrode is measured, and draws working curve；By sample to be tested
Solution replaces thyroglobulin standard solution to be detected, and the result of detection is checked in by working curve.
2. the method according to claim 1, wherein the mesoporous silicon oxide (MPS) is by following methods system
It is standby: to weigh 0.5 g cetyl trimethylammonium bromide (CTAB) and be dissolved in 240 mL ultrapure waters, 3.5 mL, 2.0 M hydrogen is added
Sodium hydroxide solution, be sufficiently stirred it is lower mixed solution is warming up to 80 °C, the positive silicon of 2.5 mL is slowly added dropwise in 30 min of insulation reaction
Acetoacetic ester (TEOS) then continues to be stirred to react 2 h under 80 °C, which is centrifuged 10 min, gained is precipitated with ultrapure
Water and ethyl alcohol respectively wash three times, and white solid is obtained after vacuum drying；Finally, 0.5 g obtained solid is taken to be added to 50 mL methanol
In the concentrated hydrochloric acid mixed solution of 2.5 mL 37wt%, 16 h are heated to reflux, filtering gained precipitating uses ultrapure water and methanol respectively
Washing three times, and under 60 °C of vacuum in dry removal duct remaining solvent to get arriving mesoporous silicon oxide.
3. the method according to claim 1, wherein the MPS-Ru (bpy)3 2+/ TEPA/TG label probe
Solution is prepared by the following method: 1) mesoporous silicon oxide adsorbs bipyridyl ruthenium [MPS-Ru (bpy)3 2+] preparation: firstly, by 50
Bipyridyl ruthenium [the Ru (bpy) of 10 mM of mesoporous silicon oxide (MPS) aqueous solution and 50 μ L of 10 mg/mL of μ L3 2+] solution shakes
10 min of mixed liquor centrifugation are obtained sediment with 100 μ L milli-Q waters and diluted by 6 h of even absorption；Then, in the solution
It is added 10 μ L 3- aminopropyl triethoxysilane (APTES) functionalization, 12 h, and with milli-Q water, 10 is added in the solution
1 h is crosslinked at 4 DEG C of 5 wt.% glutaraldehyde of μ L；2) mesoporous silicon oxide adsorbs bipyridyl ruthenium labelled antigen [MPS-Ru
(bpy)3 2+/ TEPA/TG] preparation: take the above-mentioned [MPS-Ru (bpy) of 50 μ L3 2+] solution addition 30 μ L, 1 ng/mL thyroid gland
In globulin standard solution (TG) and the mixed solution of 20 μ L, 1 mM tetraethylenepentamine (TEPA), 50 are reacted at 4 DEG C
Min, and closed with the BSA aqueous solution of 1.0 wt.%；Finally, the mixture is centrifuged 10 min, it is heavy with the 50 ultrapure water dispersions of μ L
Starch to get arrive MPS-Ru (bpy)3 2+/ TEPA/TG label probe solution is dispersed with the PBS of pH 7.4, adds 20 μ L
BSA aqueous solution, it is spare under the conditions of placing 4 °C.
4. a kind of thyroglobulin immunosensor enhanced based on mesoporous silicon oxide photo-thermal release signal, including work
It is reference electrode that electrode, platinum electrode, which are to electrode and Ag/AgCl, which is characterized in that the working electrode uses MPS-Ru
(bpy)3 2+/ TEPA/TG/Ab/MB modified electrode, the MPS-Ru (bpy)3 2+/ TEPA/TG/Ab/MB modified electrode is by following
What method was prepared: 1) polishing of glass-carbon electrode: glass-carbon electrode mechanical grinding first on the chamois leather for be covered with alumina powder
Polishing, washes away remained on surface powder with secondary water, then move into ultrasonic water bath and clean, until cleaning up, finally sequentially uses second
Alcohol, diluted acid and water thoroughly wash；2) MPS-Ru (bpy)3 2+The preparation of/TEPA/TG/Ab/MB modified electrode: by the magnetic bead of 5 mg
(MB) powder is dissolved in 1 mL, 20 wt.% ethyl alcohol, then, takes 3 μ L MB solution drop coatings in clean glassy carbon electrode surface, is drying
It dries, is cooled to room temperature in case, then take 3 μ L, the 1 water-soluble drop-coated of mM mercaptoethylmaine on the electrode, 4 °C of lower functionalization 40
min；Take 10 μ L concentration than containing 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride (EDC), N- for 4:1
The mixed solution of HOSu NHS (NHS) is added in 1 ng/mL thyroglobulin antibody (Ab) solution, at 4 DEG C
Activate 40 min；Above-mentioned modified electrode is immersed in the Ab solution after activation, 50 min are incubated under 4 °C；Then use pH 7.4
Phosphate buffer solution (PBS) remove extra Ab, electrode is finally immersed into 1 h of BSA aqueous solution that concentration is 1.0 wt.%, envelope
Nonspecific activity site on electrode surface is closed, is washed away after surface residual liquid to get to Ab/MB modified electrode；TG is configured to
The standard solution of various concentration respectively with MPS-Ru (bpy)3 2+/ TEPA/TG label probe solution is mixed to form mixed solution, and
Ab/MB modified electrode is immersed into above-mentioned mixed solution, 50 min are incubated under 4 °C, MPS-Ru is made by competitive reaction
(bpy)3 2+/ TEPA/TG label probe is incorporated in modified electrode surface；With the PBS of pH 7.0 rinse electrode surface residual solution and
Under room temperature naturally dry to get arrive MPS-Ru (bpy)3 2+/ TEPA/TG/Ab/MB modified electrode.
5. a kind of enhanced electrochemiluminescimmunosensor immunosensor of photo-thermal release signal that is based on as claimed in claim 4 is to thyroid gland
The detection method of globulin, which is characterized in that steps are as follows: 1) being measured using three-electrode system, with MPS-Ru
(bpy)3 2+/ TEPA/TG/Ab/MB modified electrode is working electrode, and Ag/AgCl is reference electrode, and platinum electrode is auxiliary electrode,
Thyroglobulin is detected on electrochemical workstation, electrochemical workstation is connected and incited somebody to action with chemiluminescence detector
The high pressure of photomultiplier tube is set as 700 V, and cyclic voltammetry scan potential range is 0 ~ 1.6 V, 0.15 V/s of sweep speed；
2) in the PBS of pH 8.0, by electrogenerated chemiluminescence method, to 1 × 10-1 μg/mL~1×10-7A series of differences of μ g/mL
The electrochemiluminescence signal intensity that the thyroglobulin standard solution of concentration generates on modified electrode is measured, and is drawn
Working curve；Thyroglobulin standard solution is replaced to detect testing sample solution, the result of detection passes through work song
Line checks in.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110716040A (en) * | 2019-10-31 | 2020-01-21 | 福建师范大学 | Preparation and application of MXene nanosheet photo-thermal amplification-based adjacent hybridization dual-mode immunosensor |
| CN112964875A (en) * | 2021-02-26 | 2021-06-15 | 福建师范大学 | Human papillomavirus 16 type E6 protein multi-mode immunoassay method based on multifunctional clinical vaginal swab |
| CN112986561A (en) * | 2021-02-26 | 2021-06-18 | 福建师范大学 | Multimode immune instant analysis excited by nano titanium carbide hybrid |
| CN113125740A (en) * | 2021-05-17 | 2021-07-16 | 郑州安图生物工程股份有限公司 | Method for identifying magnetic particle activation efficiency in chemiluminescence immunoassay technology |
| CN113484383A (en) * | 2021-07-05 | 2021-10-08 | 中国科学院长春应用化学研究所 | Nano particle film and preparation method and application thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017173453A1 (en) * | 2016-04-01 | 2017-10-05 | The Brigham And Women's Hospital, Inc. | Stimuli-responsive nanoparticles for biomedical applications |
| CN108318477A (en) * | 2018-02-05 | 2018-07-24 | 福建省妇幼保健院 | Based on TiO2Electrogenerated chemiluminescence probe prepared by metal organic frame and its competitive type immuno-sensing method to vomitoxin |
| CN108872341A (en) * | 2018-06-30 | 2018-11-23 | 福建师范大学 | A kind of preparation and application of the alpha-fetoprotein optical electro-chemistry competition immunologic pattern sensor based on Lazer's structure |
| CN109738496A (en) * | 2019-01-22 | 2019-05-10 | 重庆医科大学 | One kind is for detecting monocyte chemoattractant protein-1 novel electrochemical Biosensors preparation method |
| CN109781714A (en) * | 2019-03-04 | 2019-05-21 | 福建师范大学 | Detection method of the Ratio-type electrochemiluminescimmunosensor immunosensor based on photo-thermal enhancing to ovarian cancer markers |
| CN109799229A (en) * | 2018-12-24 | 2019-05-24 | 济南大学 | One kind being based on [Ru (bpy)3]2+The preparation method of the immunosensor of/Au@NiFe MOFs |
| CN109975375A (en) * | 2019-04-11 | 2019-07-05 | 福建师范大学 | A kind of zearalenone detection method based on signal enhancing type polymer functionalization red schorl phase titanium dioxide mesomorphic |
-
2019
- 2019-07-24 CN CN201910670775.2A patent/CN110308286B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017173453A1 (en) * | 2016-04-01 | 2017-10-05 | The Brigham And Women's Hospital, Inc. | Stimuli-responsive nanoparticles for biomedical applications |
| CN108318477A (en) * | 2018-02-05 | 2018-07-24 | 福建省妇幼保健院 | Based on TiO2Electrogenerated chemiluminescence probe prepared by metal organic frame and its competitive type immuno-sensing method to vomitoxin |
| CN108872341A (en) * | 2018-06-30 | 2018-11-23 | 福建师范大学 | A kind of preparation and application of the alpha-fetoprotein optical electro-chemistry competition immunologic pattern sensor based on Lazer's structure |
| CN109799229A (en) * | 2018-12-24 | 2019-05-24 | 济南大学 | One kind being based on [Ru (bpy)3]2+The preparation method of the immunosensor of/Au@NiFe MOFs |
| CN109738496A (en) * | 2019-01-22 | 2019-05-10 | 重庆医科大学 | One kind is for detecting monocyte chemoattractant protein-1 novel electrochemical Biosensors preparation method |
| CN109781714A (en) * | 2019-03-04 | 2019-05-21 | 福建师范大学 | Detection method of the Ratio-type electrochemiluminescimmunosensor immunosensor based on photo-thermal enhancing to ovarian cancer markers |
| CN109975375A (en) * | 2019-04-11 | 2019-07-05 | 福建师范大学 | A kind of zearalenone detection method based on signal enhancing type polymer functionalization red schorl phase titanium dioxide mesomorphic |
Non-Patent Citations (3)
| Title |
|---|
| NAN WANG等: "Fabrication of Composite Beads Based on Calcium Alginate and Tetraethylenepentamine-Functionalized MIL-101 for Adsorption of Pb(II) from Aqueous Solutions", 《POLYMERS》 * |
| 宋世昊等: "基于纳米TiO_2/壳聚糖复合膜的电化学免疫传感器制备及其在铁蛋白检测中的应用", 《湖北大学学报(自然科学版)》 * |
| 曾莉等: "石墨烯修饰的电化学免疫传感器在肿瘤标志物检测中的应用", 《检验医学与临床》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110716040A (en) * | 2019-10-31 | 2020-01-21 | 福建师范大学 | Preparation and application of MXene nanosheet photo-thermal amplification-based adjacent hybridization dual-mode immunosensor |
| CN110716040B (en) * | 2019-10-31 | 2022-06-14 | 福建师范大学 | Preparation and application of MXene nanosheet photo-thermal amplification-based adjacent hybridization dual-mode immunosensor |
| CN112964875A (en) * | 2021-02-26 | 2021-06-15 | 福建师范大学 | Human papillomavirus 16 type E6 protein multi-mode immunoassay method based on multifunctional clinical vaginal swab |
| CN112986561A (en) * | 2021-02-26 | 2021-06-18 | 福建师范大学 | Multimode immune instant analysis excited by nano titanium carbide hybrid |
| CN113125740A (en) * | 2021-05-17 | 2021-07-16 | 郑州安图生物工程股份有限公司 | Method for identifying magnetic particle activation efficiency in chemiluminescence immunoassay technology |
| CN113125740B (en) * | 2021-05-17 | 2022-03-01 | 郑州安图生物工程股份有限公司 | Method for identifying magnetic particle activation efficiency in chemiluminescence immunoassay technology |
| CN113484383A (en) * | 2021-07-05 | 2021-10-08 | 中国科学院长春应用化学研究所 | Nano particle film and preparation method and application thereof |
| CN113484383B (en) * | 2021-07-05 | 2022-10-28 | 中国科学院长春应用化学研究所 | Nano particle film and preparation method and application thereof |
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