CN105606681A - Preparation method and application of biosensor built based on gold and copper-multiwalled carbon nanotube-manganese dioxide - Google Patents
Preparation method and application of biosensor built based on gold and copper-multiwalled carbon nanotube-manganese dioxide Download PDFInfo
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Abstract
The invention relates to a preparation method and application of a biosensor built based on gold and copper-multiwalled carbon nanotube-manganese dioxide, and belongs to the technical field of novel function materials and biosensing detection. Based on the gold and copper-multiwalled carbon nanotube-manganese dioxide, the gold and copper-multiwalled carbon nanotube-manganese dioxide has good electrochemical catalysis capacity and electron transfer capacity to hydrogen peroxide, therefore, the sensitivity of the biosensor is apparently improved, and great significance for CA125 detection is achieved.
Description
Technical field
Preparation method and the application of a kind of biology sensor building based on golden copper-multi-walled carbon nano-tubes-manganese dioxide of the present invention. Specifically adopt the golden copper-multi-walled carbon nano-tubes-manganese dioxide with good electrical chemical catalysis performance, prepare the sensor of a kind of CA125 of detection, belong to new function material and bio-sensing detection technique field.
Background technology
CA125 is that detected from ovarian epithelial carcinoma antigen by Bast etc. nineteen eighty-three can be by a kind of glycoprotein of monoclonal antibody OC125 combination, derive from embryonic development period coelomic epithelium, in normal ovarian tissue, do not exist, therefore be most commonly in epithelial ovarian tumor (serous tumor) patient's serum, the sensitiveness of its diagnosis is higher, but specificity is poor. In mucus ovarian neoplasm, do not exist. 80% patients with epithelial ovarian tumor change of serum C A125 raises, but the early-stage cases of nearly half do not raise, therefore not separately for the early diagnosis of epithelial ovarian cancer. 90% patients serum CA125 is relevant with course advancement, detects and curative effect evaluation therefore be used for the state of an illness.
At present electrochemical immunosensor has been widely used in the detection of CA125 because that electrochemical immunosensor has is highly sensitive, selectively good, simple in structure, easy and simple to handle, be easy to miniaturization, can continuous, rapid automatized detection analysis etc. series of advantages. Wherein, due to the interference that unmarked electrochemical immunosensor can be directly used in the identifying of detectable antigens antibody and avoid label to bring, obtained paying close attention to more widely.
Golden copper-multi-walled carbon nano-tubes-manganese dioxide is modified glass-carbon electrode surface by the present invention, build unmarked electrochemical immunosensor, first, gold copper-multi-walled carbon nano-tubes-manganese dioxide has good catalytic effect to hydrogen peroxide, secondly, golden copper-multi-walled carbon nano-tubes-manganese dioxide has good electron transfer capacity and large specific area, last, gold copper-multi-walled carbon nano-tubes-manganese dioxide has good biocompatibility, can effectively fix a large amount of antibody. The method has produced good electrochemical signals in testing process, can be used for the analysis of CA125. The method has that cost is low, highly sensitive, specificity is good, detect the advantages such as quick, and preparation process is comparatively simple, provides new way for effectively detecting at present CA125.
Summary of the invention
One of object of the present invention is to build unmarked type biology sensor based on golden copper-multi-walled carbon nano-tubes-manganese dioxide.
Two of object of the present invention is in highly sensitive, the specific detection of CA125 by this unmarked type biosensor application.
Technical scheme of the present invention is as follows
1.A kind of preparation method of the biology sensor building based on golden copper-multi-walled carbon nano-tubes-manganese dioxide
(1) carry out polishing with the alumina powder foot couple glass-carbon electrode of 1.0,0.3,0.05 μ m successively, ultrasonic cleaning in ultra-pure water and ethanol respectively, nitrogen dries up;
(2) drip at electrode surface golden copper-multi-walled carbon nano-tubes-manganese dioxide aqueous solution that 6 μ L concentration are 1 ~ 2mg/mL, dry;
(3) continue the CA125 antibody-solutions that is 5 ~ 20 μ g/mL by 6 μ L concentration and be added drop-wise to modified electrode surface, in 4 DEG C of refrigerators, hatch 1h, clean up;
(4) seal non-specific avtive spot with the bovine serum albumin solution that 3 μ L concentration are 5 ~ 20mg/mL, in 4 DEG C of refrigerators, hatch 1h, clean up;
(5) CA125 of a series of variable concentrations that are 0.0003 ~ 30ng/mL by 6 μ L concentration is used for the specific recognition with antibody, under room temperature, hatches 1h, cleans up, and in 4 DEG C of refrigerators, stores for future use.
The preparation of gold copper-multi-walled carbon nano-tubes-manganese dioxide
In the 20mL aqueous solution, add copper sulphate and the natrium citricum that 50 μ L concentration are 0.1mol/L, adding rapidly subsequently 1mL concentration is the sodium borohydride solution of 0.95mg/mL, after 15min, add the chlorauric acid solution that 50 μ L concentration are 0.1mol/L, continue to stir 20min, obtain gold copper nano-particle solution;
50 ~ 100mg potassium permanganate is dissolved in the water of 50mL, adds after 0.1g multi-walled carbon nano-tubes, mixture is reacted to 12h in the oil bath of 100 DEG C, centrifuge washing, vacuum drying, obtains multi-walled carbon nano-tubes-manganese dioxide;
In 10mL absolute ethyl alcohol, disperse 0.1 ~ 0.3g multi-walled carbon nano-tubes-manganese dioxide, ultrasonic 5h, adds 0.2 ~ 0.4mL3-aminopropyl triethoxysilane, 70 DEG C of backflow 1.5h, and centrifuge washing, vacuum drying, makes amination multi-walled carbon nano-tubes-manganese dioxide;
8 ~ 12mg amination multi-walled carbon nano-tubes-manganese dioxide is joined in 16 ~ 25mL gold copper nano-particle solution, concussion 12h, centrifuge washing, vacuum drying, makes golden copper-multi-walled carbon nano-tubes-manganese dioxide.
The detection method of CA125
(1) use electrochemical workstation to test with three-electrode system, saturated calomel electrode is reference electrode, and platinum electrode is auxiliary electrode, and prepared sensor is working electrode, in the PBS that is 6.8, tests in the pH of 10mL value;
(2) select chronoamperometry to detect CA125, be set to-0.4V of input voltage, sampling interval is set to 0.1s, is set to 400s running time;
(3) after background current tends towards stability, in PBS, inject the hydrogen peroxide solution that 10 μ L concentration are 5mol/L every 50s, then record current over time, drawing curve;
(4) testing sample solution is replaced CA125 standard liquid detect.
Useful achievement of the present invention
(1) the present invention adopts golden copper-multi-walled carbon nano-tubes-manganese dioxide to have good biocompatibility, by forming golden nitrogen key chemical bonding to glass-carbon electrode surface.
(2) golden copper-multi-walled carbon nano-tubes-manganese dioxide that the present invention adopts has large specific area, can effectively fix a large amount of antibody.
(3) golden copper-multi-walled carbon nano-tubes-manganese dioxide that the present invention adopts has superior electrochemical catalysis performance, the sensitivity that has improved biology sensor to hydrogen peroxide.
(4) golden copper-multi-walled carbon nano-tubes-manganese dioxide that the present invention adopts has the transmission efficiency of good electronics, the sensitivity that has further improved biology sensor.
(5) the present invention's detection for CA125 by the unmarked type biology sensor of preparation, detectability is low, and the range of linearity is wide, can realize simple, quick, sensitive and specific detection.
Detailed description of the invention
Embodiment 1A kind of preparation method of the biology sensor building based on golden copper-multi-walled carbon nano-tubes-manganese dioxide
(1) carry out polishing with the alumina powder foot couple glass-carbon electrode of 1.0,0.3,0.05 μ m successively, ultrasonic cleaning in ultra-pure water and ethanol respectively, nitrogen dries up;
(2) drip at electrode surface golden copper-multi-walled carbon nano-tubes-manganese dioxide aqueous solution that 6 μ L concentration are 1mg/mL, dry;
(3) continue the CA125 antibody-solutions that is 5 μ g/mL by 6 μ L concentration and be added drop-wise to modified electrode surface, in 4 DEG C of refrigerators, hatch 1h, clean up;
(4) seal non-specific avtive spot with the bovine serum albumin solution that 3 μ L concentration are 5mg/mL, in 4 DEG C of refrigerators, hatch 1h, clean up;
(5) CA125 of a series of variable concentrations that are 0.0003 ~ 30ng/mL by 6 μ L concentration is used for the specific recognition with antibody, under room temperature, hatches 1h, cleans up, and in 4 DEG C of refrigerators, stores for future use.
Embodiment 2A kind of preparation method of the biology sensor building based on golden copper-multi-walled carbon nano-tubes-manganese dioxide
(1) carry out polishing with the alumina powder foot couple glass-carbon electrode of 1.0,0.3,0.05 μ m successively, ultrasonic cleaning in ultra-pure water and ethanol respectively, nitrogen dries up;
(2) drip at electrode surface golden copper-multi-walled carbon nano-tubes-manganese dioxide aqueous solution that 6 μ L concentration are 1.5mg/mL, dry;
(3) continue the CA125 antibody-solutions that is 10 μ g/mL by 6 μ L concentration and be added drop-wise to modified electrode surface, in 4 DEG C of refrigerators, hatch 1h, clean up;
(4) seal non-specific avtive spot with the bovine serum albumin solution that 3 μ L concentration are 10mg/mL, in 4 DEG C of refrigerators, hatch 1h, clean up;
(5) CA125 of a series of variable concentrations that are 0.0003 ~ 30ng/mL by 6 μ L concentration is used for the specific recognition with antibody, under room temperature, hatches 1h, cleans up, and in 4 DEG C of refrigerators, stores for future use.
Embodiment 3A kind of preparation method of the biology sensor building based on golden copper-multi-walled carbon nano-tubes-manganese dioxide
(1) carry out polishing with the alumina powder foot couple glass-carbon electrode of 1.0,0.3,0.05 μ m successively, ultrasonic cleaning in ultra-pure water and ethanol respectively, nitrogen dries up;
(2) drip at electrode surface golden copper-multi-walled carbon nano-tubes-manganese dioxide aqueous solution that 6 μ L concentration are 2mg/mL, dry;
(3) continue the CA125 antibody-solutions that is 20 μ g/mL by 6 μ L concentration and be added drop-wise to modified electrode surface, in 4 DEG C of refrigerators, hatch 1h, clean up;
(4) seal non-specific avtive spot with the bovine serum albumin solution that 3 μ L concentration are 20mg/mL, in 4 DEG C of refrigerators, hatch 1h, clean up;
(5) CA125 of a series of variable concentrations that are 0.0003 ~ 30ng/mL by 6 μ L concentration is used for the specific recognition with antibody, under room temperature, hatches 1h, cleans up, and in 4 DEG C of refrigerators, stores for future use.
Embodiment 4The preparation of gold copper-multi-walled carbon nano-tubes-manganese dioxide
In the 20mL aqueous solution, add copper sulphate and the natrium citricum that 50 μ L concentration are 0.1mol/L, adding rapidly subsequently 1mL concentration is the sodium borohydride solution of 0.95mg/mL, after 15min, add the chlorauric acid solution that 50 μ L concentration are 0.1mol/L, continue to stir 20min, obtain gold copper nano-particle solution;
50mg potassium permanganate is dissolved in the water of 50mL, adds after 0.1g multi-walled carbon nano-tubes, mixture is reacted to 12h in the oil bath of 100 DEG C, centrifuge washing, vacuum drying, obtains multi-walled carbon nano-tubes-manganese dioxide;
In 10mL absolute ethyl alcohol, disperse 0.1g multi-walled carbon nano-tubes-manganese dioxide, ultrasonic 5h, adds 0.2mL3-aminopropyl triethoxysilane, 70 DEG C of backflow 1.5h, and centrifuge washing, vacuum drying, makes amination multi-walled carbon nano-tubes-manganese dioxide;
8mg amination multi-walled carbon nano-tubes-manganese dioxide is joined in 16mL gold copper nano-particle solution, concussion 12h, centrifuge washing, vacuum drying, makes golden copper-multi-walled carbon nano-tubes-manganese dioxide.
Embodiment 5The preparation of gold copper-multi-walled carbon nano-tubes-manganese dioxide
In the 20mL aqueous solution, add copper sulphate and the natrium citricum that 50 μ L concentration are 0.1mol/L, adding rapidly subsequently 1mL concentration is the sodium borohydride solution of 0.95mg/mL, after 15min, add the chlorauric acid solution that 50 μ L concentration are 0.1mol/L, continue to stir 20min, obtain gold copper nano-particle solution;
75mg potassium permanganate is dissolved in the water of 50mL, adds after 0.1g multi-walled carbon nano-tubes, mixture is reacted to 12h in the oil bath of 100 DEG C, centrifuge washing, vacuum drying, obtains multi-walled carbon nano-tubes-manganese dioxide;
In 10mL absolute ethyl alcohol, disperse 0.2g multi-walled carbon nano-tubes-manganese dioxide, ultrasonic 5h, adds 0.3mL3-aminopropyl triethoxysilane, 70 DEG C of backflow 1.5h, and centrifuge washing, vacuum drying, makes amination multi-walled carbon nano-tubes-manganese dioxide;
10mg amination multi-walled carbon nano-tubes-manganese dioxide is joined in 20mL gold copper nano-particle solution, concussion 12h, centrifuge washing, vacuum drying, makes golden copper-multi-walled carbon nano-tubes-manganese dioxide.
Embodiment 6The preparation of gold copper-multi-walled carbon nano-tubes-manganese dioxide
In the 20mL aqueous solution, add copper sulphate and the natrium citricum that 50 μ L concentration are 0.1mol/L, adding rapidly subsequently 1mL concentration is the sodium borohydride solution of 0.95mg/mL, after 15min, add the chlorauric acid solution that 50 μ L concentration are 0.1mol/L, continue to stir 20min, obtain gold copper nano-particle solution;
100mg potassium permanganate is dissolved in the water of 50mL, adds after 0.1g multi-walled carbon nano-tubes, mixture is reacted to 12h in the oil bath of 100 DEG C, centrifuge washing, vacuum drying, obtains multi-walled carbon nano-tubes-manganese dioxide;
In 10mL absolute ethyl alcohol, disperse 0.3g multi-walled carbon nano-tubes-manganese dioxide, ultrasonic 5h, adds 0.4mL3-aminopropyl triethoxysilane, 70 DEG C of backflow 1.5h, and centrifuge washing, vacuum drying, makes amination multi-walled carbon nano-tubes-manganese dioxide;
12mg amination multi-walled carbon nano-tubes-manganese dioxide is joined in 25mL gold copper nano-particle solution, concussion 12h, centrifuge washing, vacuum drying, makes golden copper-multi-walled carbon nano-tubes-manganese dioxide.
Embodiment 7The detection method of CA125
(1) use electrochemical workstation to test with three-electrode system, saturated calomel electrode is reference electrode, and platinum electrode is auxiliary electrode, and prepared sensor is working electrode, in the PBS that is 6.8, tests in the pH of 10mL value;
(2) select chronoamperometry to detect CA125, be set to-0.4V of input voltage, sampling interval is set to 0.1s, is set to 400s running time;
(3) after background current tends towards stability, in PBS, inject the hydrogen peroxide solution that 10 μ L concentration are 5mol/L every 50s, then record current over time, drawing curve;
(4) testing sample solution is replaced CA125 standard liquid detect;
(5) this biology sensor is 0.0003 ~ 30ng/mL to the CA125 detection range of linearity, detectability 0.15pg/mL.
Claims (3)
1. a preparation method for the biology sensor building based on golden copper-multi-walled carbon nano-tubes-manganese dioxide, is characterized in that, step is as follows:
(1) carry out polishing with the alumina powder foot couple glass-carbon electrode of 1.0,0.3,0.05 μ m successively, ultrasonic cleaning in ultra-pure water and ethanol respectively, nitrogen dries up;
(2) drip at electrode surface golden copper-multi-walled carbon nano-tubes-manganese dioxide aqueous solution that 6 μ L concentration are 1 ~ 2mg/mL, dry;
(3) continue the CA125 antibody-solutions that is 5 ~ 20 μ g/mL by 6 μ L concentration and be added drop-wise to modified electrode surface, in 4 DEG C of refrigerators, hatch 1h, clean up;
(4) seal non-specific avtive spot with the bovine serum albumin solution that 3 μ L concentration are 5 ~ 20mg/mL, in 4 DEG C of refrigerators, hatch 1h, clean up;
(5) CA125 of a series of variable concentrations that are 0.0003 ~ 30ng/mL by 6 μ L concentration is used for the specific recognition with antibody, under room temperature, hatches 1h, cleans up, and in 4 DEG C of refrigerators, stores for future use.
2. the preparation method of a kind of biology sensor building based on golden copper-multi-walled carbon nano-tubes-manganese dioxide as claimed in claim 1, the preparation of described golden copper-multi-walled carbon nano-tubes-manganese dioxide, is characterized in that, step is as follows:
In the 20mL aqueous solution, add copper sulphate and the natrium citricum that 50 μ L concentration are 0.1mol/L, adding rapidly subsequently 1mL concentration is the sodium borohydride solution of 0.95mg/mL, after 15min, add the chlorauric acid solution that 50 μ L concentration are 0.1mol/L, continue to stir 20min, obtain gold copper nano-particle solution;
50 ~ 100mg potassium permanganate is dissolved in the water of 50mL, adds after 0.1g multi-walled carbon nano-tubes, mixture is reacted to 12h in the oil bath of 100 DEG C, centrifuge washing, vacuum drying, obtains multi-walled carbon nano-tubes-manganese dioxide;
In 10mL absolute ethyl alcohol, disperse 0.1 ~ 0.3g multi-walled carbon nano-tubes-manganese dioxide, ultrasonic 5h, adds 0.2 ~ 0.4mL3-aminopropyl triethoxysilane, 70 DEG C of backflow 1.5h, and centrifuge washing, vacuum drying, makes amination multi-walled carbon nano-tubes-manganese dioxide;
8 ~ 12mg amination multi-walled carbon nano-tubes-manganese dioxide is joined in 16 ~ 25mL gold copper nano-particle solution, concussion 12h, centrifuge washing, vacuum drying, makes golden copper-multi-walled carbon nano-tubes-manganese dioxide.
3. the detection method of a kind of biology sensor building based on golden copper-multi-walled carbon nano-tubes-manganese dioxide that prepared by preparation method as claimed in claim 1 to CA125, is characterized in that, step is as follows:
(1) use electrochemical workstation to test with three-electrode system, saturated calomel electrode is reference electrode, and platinum electrode is auxiliary electrode, and prepared sensor is working electrode, in the PBS that is 6.8, tests in the pH of 10mL value;
(2) select chronoamperometry to detect CA125, be set to-0.4V of input voltage, sampling interval is set to 0.1s, is set to 400s running time;
(3) after background current tends towards stability, in PBS, inject the hydrogen peroxide solution that 10 μ L concentration are 5mol/L every 50s, then record current over time, drawing curve;
(4) testing sample solution is replaced CA125 standard liquid detect.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106442994A (en) * | 2016-09-14 | 2017-02-22 | 山东理工大学 | Preparation method and application of electrochemical immunosensor based on Ag@Au nanocomposite |
| CN108246292A (en) * | 2017-12-15 | 2018-07-06 | 安阳师范学院 | Nanogold/manganese dioxide/graphene-carbon nano tube three-dimensional structure nano-complex and the hydrogen peroxide sensor with its making |
| CN109115855A (en) * | 2018-11-07 | 2019-01-01 | 山东理工大学 | A kind of preparation method and application for the electrochemical immunosensor detecting Alzheimer's disease marker |
| CN109126822A (en) * | 2018-09-25 | 2019-01-04 | 中南大学 | A kind of carbon nanotube-gold copper composite material and preparation method and application |
| CN112067676A (en) * | 2020-08-03 | 2020-12-11 | 南昌大学 | Preparation and detection method of sensor for detecting inorganic phosphate in fresh water |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104391113A (en) * | 2014-12-17 | 2015-03-04 | 济南大学 | Preparation method and application of biosensor built based on gold copper nanowires and 3-mercaptopropyltriethoxysilane graphene |
| CN104407138A (en) * | 2014-11-12 | 2015-03-11 | 济南大学 | Preparation method and application of biosensor based on pine-branch-shaped nano-copper |
| CN104569427A (en) * | 2014-12-30 | 2015-04-29 | 山东理工大学 | Preparation method and application of immunosensor established based on manganese dioxide loaded silver nanoparticle multiwalled carbon nanotube |
| CN104897757A (en) * | 2015-04-29 | 2015-09-09 | 济南大学 | Production method and application of PdNi alloy/nitrogen doped graphene nanoribbon dual-amplification immunosensor |
| CN105044341A (en) * | 2015-07-03 | 2015-11-11 | 济南大学 | Preparation method and applications of electrodeposited gold and gold compound stomach cancer marker CA724 biosensor |
-
2015
- 2015-12-27 CN CN201510987739.0A patent/CN105606681B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104407138A (en) * | 2014-11-12 | 2015-03-11 | 济南大学 | Preparation method and application of biosensor based on pine-branch-shaped nano-copper |
| CN104391113A (en) * | 2014-12-17 | 2015-03-04 | 济南大学 | Preparation method and application of biosensor built based on gold copper nanowires and 3-mercaptopropyltriethoxysilane graphene |
| CN104569427A (en) * | 2014-12-30 | 2015-04-29 | 山东理工大学 | Preparation method and application of immunosensor established based on manganese dioxide loaded silver nanoparticle multiwalled carbon nanotube |
| CN104897757A (en) * | 2015-04-29 | 2015-09-09 | 济南大学 | Production method and application of PdNi alloy/nitrogen doped graphene nanoribbon dual-amplification immunosensor |
| CN105044341A (en) * | 2015-07-03 | 2015-11-11 | 济南大学 | Preparation method and applications of electrodeposited gold and gold compound stomach cancer marker CA724 biosensor |
Non-Patent Citations (3)
| Title |
|---|
| ÇAGRI CEYLAN BAKIR等: "Electrocatalytic reduction of oxygen on bimetallic copper–gold nanoparticles–multiwalled carbon nanotube modified glassy carbon electrode in alkaline solution", 《JOURNAL OF ELECTROANALYTICAL CHEMISTRY》 * |
| CHARLOTTE L. BRACEY等: "Application of copper–gold alloys in catalysis: current status and future perspectives", 《CHEM.SOC.REV.》 * |
| MENG-CHE TU等: "Immunosensor based on carbon nanotube/manganese dioxide electrochemical tags", 《ANALYTICA CHIMICA ACTA》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106442994A (en) * | 2016-09-14 | 2017-02-22 | 山东理工大学 | Preparation method and application of electrochemical immunosensor based on Ag@Au nanocomposite |
| CN106442994B (en) * | 2016-09-14 | 2018-01-23 | 山东理工大学 | A kind of preparation method and application of the electrochemical immunosensor based on Ag@Au nano composite materials |
| CN108246292A (en) * | 2017-12-15 | 2018-07-06 | 安阳师范学院 | Nanogold/manganese dioxide/graphene-carbon nano tube three-dimensional structure nano-complex and the hydrogen peroxide sensor with its making |
| CN109126822A (en) * | 2018-09-25 | 2019-01-04 | 中南大学 | A kind of carbon nanotube-gold copper composite material and preparation method and application |
| CN109126822B (en) * | 2018-09-25 | 2021-05-07 | 中南大学 | Carbon nanotube-gold copper alloy composite material and preparation method and application thereof |
| CN109115855A (en) * | 2018-11-07 | 2019-01-01 | 山东理工大学 | A kind of preparation method and application for the electrochemical immunosensor detecting Alzheimer's disease marker |
| CN112067676A (en) * | 2020-08-03 | 2020-12-11 | 南昌大学 | Preparation and detection method of sensor for detecting inorganic phosphate in fresh water |
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