CN104931698A - Preparation method and application of NP-NiGd@Au-based gastric cancer marker gold nano-cluster electrogenerated chemiluminescence sensor - Google Patents
Preparation method and application of NP-NiGd@Au-based gastric cancer marker gold nano-cluster electrogenerated chemiluminescence sensor Download PDFInfo
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Abstract
The invention relates to a preparation method and application of an NP-NiGd@Au-based gastric cancer marker gold nano-cluster electrogenerated chemiluminescence sensor, and belongs to the field of electrochemiluminescence sensors. The gold nano-cluster is taken as an electrochemical luminescence signal source; carrier content of antibody is increased due to excellent biocompatibility and large specific surface area of the nano porous material NP-NiGd@Au; graphene oxide is used for immobilizing the gold nano-cluster AuNCs@BSA to serve as a secondary antibody marker. Detection of gastric cancer marker is realized based on different electrochemical luminescence signal strengths of to-be-detected objects of different concentrations.
Description
Technical field
The present invention relates to a kind of preparation method and application of the stomach cancer marker gold nanoclusters Electrochemiluminescsensor sensor based on NP-NiGdAu.Be specifically related to a kind of AuNCsBSA as luminescent material, the bio-compatibility utilizing nano-porous materials NP-NiGdAu excellent and large specific surface area increase the supported quantity of antibody as base material.With GO/AuNCsBSA as two anti-labels, belong to electrochemiluminescence detection technique field.
Background technology
Cancer of the stomach is in its generation and evolution, and stomach cancer cell can produce the materials such as enzyme, isoenzymes, hormone and immunoglobulin (Ig) usually, and these materials relevant to cancer of the stomach are called as stomach cancer marker.These marks do not exist only in cell or cell surface, but also blood or body cavity can be secreted into, therefore stomach cancer marker can be measured in body fluid or mucous membrane tissue, for early warning or auxiliary diagnosis, the analysis course of disease, guiding treatment, monitoring recurrence or transfer, the judging prognosis etc. of cancer of the stomach.
The analytical approach of current detection stomach cancer marker mainly contains radio immunoassay, enzyme-linked immunosorbent assay and RNA isolation kit, but the reagent term of validity used is short, has radioactive contamination, the shortcomings such as sense cycle is long, and sensitivity is low, complex steps.In order to overcome the shortcoming of above traditional analysis, the present invention devises a kind of high specificity, highly sensitive, no radioactivity pollute, operates fast and convenient electrogenerated chemiluminescence immune analysis method.
Summary of the invention
The object of the invention is for existing stomach cancer marker detection method Problems existing, there is provided a kind of simple and quick reliably based on the preparation method and application of the electrochemical luminous sensor of NP-NiGdAu and GO/AuNCsBSA, realize quick, sensitive, special, the efficient detection to stomach cancer marker.
technical scheme of the present invention is as follows:
Based on a preparation method for the stomach cancer marker gold nanoclusters Electrochemiluminescsensor sensor of NP-NiGdAu, comprise the following steps:
(1) glass-carbon electrode of diameter 4 mm is taken turns doing polishing with 1.0 μm, 0.3 μm, 0.05 μm aluminum oxide polishing powder successively, clean with ultrapure water;
(2) painting 6 μ L, 0.5 ~ 2 mg mL is dripped
-1capture antibody base material NP-NiGdAu/Ab
1solution and electrode surface, 4 DEG C are saved to drying;
(3) drip 4 μ L, massfraction is the bovine serum albumin solution of 1 ~ 3%, with the nonspecific activity site on enclosed-electrode surface, rinse electrode surface by the PBS solution of pH6.4 ~ 8.4,4 DEG C are dried;
(4) drip the determinand antigen of 6 μ L variable concentrations, hatch 30 min at 4 DEG C, rinse electrode surface by the PBS solution of pH6.4 ~ 8.4,4 DEG C are dried;
(5) the anti-label GO/AuNCsBSA/Ab of painting 6 μ L bis-is dripped
2solution, rinse electrode surface by the PBS solution of pH6.4 ~ 8.4,4 DEG C are dried.
2. capture antibody base material NP-NiGdAu/Ab
1the preparation of solution
(1) preparation of NP-NiGdAu
NiGdAl alloy is put into the sodium hydroxide solution of 1.5 ~ 2.5 excessive mol/L, at room temperature chemical corrosion 32 ~ 74 h, centrifuge washing is to neutral, and in vacuum drying chamber, dry 12 ~ 48 h, obtain nanoporous NiGd;
By 80 ~ 120 mL, massfraction be 0.005 ~ 0.015% chlorauric acid solution boil, add 2 ~ 3 mL, massfraction is the trisodium citrate aqueous solution of 0.5 ~ 1.5%, add hot reflux 10 ~ 20 min, treat that solution colour becomes claret, solution is cooled to room temperature, the golden nanometer particle obtained, keeps in Dark Place at 4 DEG C.
The NP-NiGd of 1 ~ 3 mg is distributed in 1 mL ultrapure water, add 0.5 ~ 1.5 mL golden nanometer particle, the mixed solution obtained at room temperature vibrates 12 ~ 32 h, centrifuging, remove excessive golden nanometer particle, and product NP-NiGdAu is re-dispersed in 1 mL ultrapure water, obtained base material NP-NiGdAu solution;
(2) capture antibody base material NP-NiGdAu/Ab
1the preparation of solution
In base material NP-NiGdAu solution, add the determinand antibody A b of 10 ~ 100 μ L, 1 mg/mL
1solution, vibrate hatching 24 h at 4 DEG C, the determinand antibody A b that centrifugal removing is excessive
1, product is distributed in the PBS solution of 1 mL, pH 7.4, obtained capture antibody base material NP-NiGdAu/Ab
1solution, for subsequent use at being stored in 4 DEG C.
3. two anti-label GO/AuNCsBSA/Ab
2the preparation of solution
(1) preparation of graphene oxide GO
0.2 ~ 0.4 g graphite and 1.5 ~ 2.1 g potassium permanganate are joined in the 500 mL there-necked flasks with magneton, add the concentrated sulphuric acid and strong phosphoric acid mixed liquor that the volume ratio mixed in advance is 9:1, 6 ~ 24 h are reacted at 30 ~ 70 DEG C, after reaction terminates, sample is poured on the ice cube of 30 ~ 50 mL frozen in advance, 200 ~ 400 μ L are added under stirring slowly, massfraction is the hydrogen peroxide of 30%, reaction 20 ~ 40 min, centrifuging, and use the hydrochloric acid solution of 0.2 mol/L successively, ethanol washs three times, finally use ether centrifuge washing once, the solid obtained vacuum drying at 35 DEG C, obtain the pressed powder of brown color,
(2) preparation of gold nanoclusters AuNCsBSA
0.3 ~ 0.8 mmol gold chloride ethanolic solution and 0.5 mmol mercapto succinic acid MSA are mixed with 100 mL methyl alcohol by the method for logical nitrogen bubble, dropwise add 20 ~ 30 mL with vigorous stirring, the sodium borohydride solution of the fresh configuration of 0.1 ~ 0.3 mol/L, the dark-brown precipitation generated, centrifuging is also washed with water/ethanolic solution that volume ratio is 1:4, dry under vacuo, obtain MSAAuNCs solid;
MSAAuNCs and BSA is that the ratio of 1:10 adds in 5 mL water with mass ratio, stirs 3 ~ 8 min, stirring 6 ~ 8 h at being 12,37 DEG C with the sodium hydrate regulator solution pH of 1 ~ 3 mol/L, and the product obtained is dialysed 24 h, and freeze drying obtains AuNCsBSA;
(3) two anti-label GO/AuNCsBSA/Ab
2the preparation of solution
The graphene oxide GO of 1 ~ 3 mg and the AuNCsBSA of 2 mg is added in 2 mL ultrapure waters, vibration 32 ~ 72 h, centrifugal and be re-dispersed into 1 mL, in the PBS buffer solution of pH7.4, add 0.05 ~ 0.15 mg determinand antibody A b
2, at 4 DEG C, hatch 12 ~ 32 h, centrifugal and be re-dispersed into 1 mL, in the PBS buffer solution of pH 7.4, be stored in 4 DEG C for subsequent use.
4. the detection method of determinand
(1) three-electrode system of electrochemical workstation is used to test, Ag/AgCl electrode is as contrast electrode, platinum electrode is to electrode, prepared electrochemical luminous sensor is working electrode, electrochemical workstation and chemiluminescence detector are linked together the high pressure of photomultiplier is set to 800 V, cyclic voltammetry scan potential range is-1.8V ~-0.6V, and sweep speed is 0.1 V/s;
(2) containing in the PBS solution of 26 ~ 46 mmol/L potassium persulfates at 10 mL, pH 6.4 ~ 8.4, by electrochemical luminescence system, the electrochemical luminescence signals intensity that the determinand antigen of variable concentrations is produced is detected, drawing curve;
(3) determinand antigen is replaced by testing sample solution to detect.
5. determinand described above, is selected from one of following gastric cancer antigen: CEA, CA199, CA50, CA724, CA195, CA242, CYFRA2H.
useful achievement of the present invention
(1) electrochemical luminous sensor preparation method, is luminescent material with AuNCsBSA, utilizes the optical property that AuNCsBSA is good, and the sensor of structure has higher sensitivity;
(2) using NP-NiGdAu as capture antibody substrate, GO/AuNCsBSA is as two anti-labels, and the advantages such as NP-NiGdAu and GO/AuNCsBSA bio-compatibility is good, and specific surface area is large effectively increase the supported quantity of antibody.
(3) electrochemical luminous sensor prepared of the present invention is for the detection of stomach cancer marker, simple to operate, and fast, signal response wide ranges, can realize simple, quick, sensitive, specific detection in reaction.
embodiment 1a kind of preparation method of the stomach cancer marker gold nanoclusters Electrochemiluminescsensor sensor based on NP-NiGdAu
(1) glass-carbon electrode of diameter 4 mm is taken turns doing polishing with 1.0 μm, 0.3 μm, 0.05 μm aluminum oxide polishing powder successively, clean with ultrapure water;
(2) painting 6 μ L, 0.5 mg mL is dripped
-1capture antibody base material NP-NiGdAu/Ab
1solution and electrode surface, 4 DEG C are saved to drying;
(3) drip 4 μ L, massfraction is the bovine serum albumin solution of 1%, with the nonspecific activity site on enclosed-electrode surface, rinse electrode surface by the PBS solution of pH 6.4,4 DEG C are dried;
(4) drip the determinand antigen of 6 μ L variable concentrations, hatch 30 min at 4 DEG C, rinse electrode surface by the PBS solution of pH 6.4,4 DEG C are dried;
(5) the anti-label GO/AuNCsBSA/Ab of painting 6 μ L bis-is dripped
2solution, rinse electrode surface by the PBS solution of pH 6.4,4 DEG C are dried.
embodiment 2a kind of preparation method of the stomach cancer marker gold nanoclusters Electrochemiluminescsensor sensor based on NP-NiGdAu
(1) glass-carbon electrode of diameter 4 mm is taken turns doing polishing with 1.0 μm, 0.3 μm, 0.05 μm aluminum oxide polishing powder successively, clean with ultrapure water;
(2) painting 6 μ L, 1 mg mL is dripped
-1capture antibody base material NP-NiGdAu/Ab
1solution and electrode surface, 4 DEG C are saved to drying;
(3) drip 4 μ L, massfraction is the bovine serum albumin solution of 2%, with the nonspecific activity site on enclosed-electrode surface, rinse electrode surface by the PBS solution of pH 7.4,4 DEG C are dried;
(4) drip the determinand antigen of 6 μ L variable concentrations, hatch 30 min at 4 DEG C, rinse electrode surface by the PBS solution of pH 7.4,4 DEG C are dried;
(5) the anti-label GO/AuNCsBSA/Ab of painting 6 μ L bis-is dripped
2solution, rinse electrode surface by the PBS solution of pH 7.4,4 DEG C are dried.
embodiment 3a kind of preparation method of the stomach cancer marker gold nanoclusters Electrochemiluminescsensor sensor based on NP-NiGdAu
(1) glass-carbon electrode of diameter 4 mm is taken turns doing polishing with 1.0 μm, 0.3 μm, 0.05 μm aluminum oxide polishing powder successively, clean with ultrapure water;
(2) painting 6 μ L, 2 mg mL are dripped
-1capture antibody base material NP-NiGdAu/Ab
1solution and electrode surface, 4 DEG C are saved to drying;
(3) drip 4 μ L, massfraction is the bovine serum albumin solution of 3%, with the nonspecific activity site on enclosed-electrode surface, rinse electrode surface by the PBS solution of pH 8.4,4 DEG C are dried;
(4) drip the determinand antigen of 6 μ L variable concentrations, hatch 30 min at 4 DEG C, rinse electrode surface by the PBS solution of pH 8.4,4 DEG C are dried;
(5) the anti-label GO/AuNCsBSA/Ab of painting 6 μ L bis-is dripped
2solution, rinse electrode surface by the PBS solution of pH 8.4,4 DEG C are dried.
embodiment 4capture antibody base material NP-NiGdAu/Ab
1the preparation of solution
(1) preparation of NP-NiGdAu
NiGdAl alloy is put into the sodium hydroxide solution of 1.5 excessive mol/L, at room temperature chemical corrosion 32 h, centrifuge washing is to neutral, and dry 12 h in vacuum drying chamber, obtain nanoporous NiGd;
By 80 mL, massfraction be 0.005% chlorauric acid solution boil, add 2 mL, massfraction is the trisodium citrate aqueous solution of 0.5%, adds hot reflux 10 min, treats that solution colour becomes claret, solution is cooled to room temperature, the golden nanometer particle obtained, keeps in Dark Place at 4 DEG C.
The NP-NiGd of 1 mg is distributed in 1 mL ultrapure water, add 0.5 mL golden nanometer particle, the mixed solution obtained at room temperature vibrates 12 h, centrifuging, remove excessive golden nanometer particle, and product NP-NiGdAu is re-dispersed in 1 mL ultrapure water, obtained base material NP-NiGdAu solution;
(2) capture antibody base material NP-NiGdAu/Ab
1the preparation of solution
In base material NP-NiGdAu solution, add the determinand antibody A b of 10 μ L, 1 mg/mL
1solution, vibrate hatching 24 h at 4 DEG C, the determinand antibody A b that centrifugal removing is excessive
1, product is distributed in the PBS solution of 1 mL, pH 7.4, obtained capture antibody base material NP-NiGdAu/Ab
1solution, for subsequent use at being stored in 4 DEG C.
embodiment 5capture antibody base material NP-NiGdAu/Ab
1the preparation of solution
(1) preparation of NP-NiGdAu
NiGdAl alloy is put into the sodium hydroxide solution of 2 excessive mol/L, at room temperature chemical corrosion 48 h, centrifuge washing is to neutral, and dry 32 h in vacuum drying chamber, obtain nanoporous NiGd;
By 100 mL, massfraction be 0.01% chlorauric acid solution boil, add 2.5 mL, massfraction is the trisodium citrate aqueous solution of 1%, adds hot reflux 15 min, treats that solution colour becomes claret, solution is cooled to room temperature, the golden nanometer particle obtained, keeps in Dark Place at 4 DEG C.
The NP-NiGd of 2 mg is distributed in 1 mL ultrapure water, add 1 mL golden nanometer particle, the mixed solution obtained at room temperature vibrates 24 h, centrifuging, remove excessive golden nanometer particle, and product NP-NiGdAu is re-dispersed in 1 mL ultrapure water, obtained base material NP-NiGdAu solution;
(2) capture antibody base material NP-NiGdAu/Ab
1the preparation of solution
In base material NP-NiGdAu solution, add the determinand antibody A b of 50 μ L, 1 mg/mL
1solution, vibrate hatching 24 h at 4 DEG C, the determinand antibody A b that centrifugal removing is excessive
1, product is distributed in the PBS solution of 1 mL, pH 7.4, obtained capture antibody base material NP-NiGdAu/Ab
1solution, for subsequent use at being stored in 4 DEG C.
embodiment 6capture antibody base material NP-NiGdAu/Ab
1the preparation of solution
(1) preparation of NP-NiGdAu
NiGdAl alloy is put into the sodium hydroxide solution of 2.5 excessive mol/L, at room temperature chemical corrosion 74 h, centrifuge washing is to neutral, and dry 48 h in vacuum drying chamber, obtain nanoporous NiGd;
By 120 mL, massfraction be 0.015% chlorauric acid solution boil, add 3 mL, massfraction is the trisodium citrate aqueous solution of 1.5%, adds hot reflux 20 min, treats that solution colour becomes claret, solution is cooled to room temperature, the golden nanometer particle obtained, keeps in Dark Place at 4 DEG C.
The NP-NiGd of 3 mg is distributed in 1 mL ultrapure water, add 1.5 mL golden nanometer particles, the mixed solution obtained at room temperature vibrates 32 h, centrifuging, remove excessive golden nanometer particle, and product NP-NiGdAu is re-dispersed in 1 mL ultrapure water, obtained base material NP-NiGdAu solution;
(2) capture antibody base material NP-NiGdAu/Ab
1the preparation of solution
In base material NP-NiGdAu solution, add the determinand antibody A b of 100 μ L, 1 mg/mL
1solution, vibrate hatching 24 h at 4 DEG C, the determinand antibody A b that centrifugal removing is excessive
1, product is distributed in the PBS solution of 1 mL, pH 7.4, obtained capture antibody base material NP-NiGdAu/Ab
1solution, for subsequent use at being stored in 4 DEG C.
embodiment 7two anti-label GO/AuNCsBSA/Ab
2the preparation of solution
(1) preparation of graphene oxide GO
0.2 g graphite and 1.5 g potassium permanganate are joined in the 500 mL there-necked flasks with magneton, add the concentrated sulphuric acid and strong phosphoric acid mixed liquor that the volume ratio mixed in advance is 9:1, 6 h are reacted at 30 DEG C, after reaction terminates, sample is poured on the ice cube of 30 mL frozen in advance, 200 μ L are added under stirring slowly, massfraction is the hydrogen peroxide of 30%, react 20 min, centrifuging, and use the hydrochloric acid solution of 0.2 mol/L successively, ethanol washs three times, finally use ether centrifuge washing once, the solid obtained vacuum drying at 35 DEG C, obtain the pressed powder of brown color,
(2) preparation of gold nanoclusters AuNCsBSA
0.3 mmol gold chloride ethanolic solution and 0.5 mmol mercapto succinic acid MSA are mixed with 100 mL methyl alcohol by the method for logical nitrogen bubble, dropwise add 20 mL with vigorous stirring, the sodium borohydride solution of the fresh configuration of 0.1 mol/L, the dark-brown precipitation generated, centrifuging is also washed with water/ethanolic solution that volume ratio is 1:4, dry under vacuo, obtain MSAAuNCs solid;
MSAAuNCs and BSA is that the ratio of 1:10 adds in 5 mL water with mass ratio, stirs 3 min, stirs 6 hs at being 12,37 DEG C with the sodium hydrate regulator solution pH of 1 mol/L, and the product obtained is dialysed 24 h, and freeze drying obtains AuNCsBSA;
(3) two anti-label GO/AuNCsBSA/Ab
2the preparation of solution
Added in 2 mL ultrapure waters by the graphene oxide GO of 1 mg and the AuNCsBSA of 2 mg, vibrate 32 h, centrifugal and be re-dispersed into 1 mL, in the PBS buffer solution of pH7.4, adds 0.05 mg determinand antibody A b
2, at 4 DEG C, hatch 12 h, centrifugal and be re-dispersed into 1 mL, in the PBS buffer solution of pH 7.4, be stored in 4 DEG C for subsequent use.
embodiment 8two anti-label GO/AuNCsBSA/Ab
2the preparation of solution
(1) preparation of graphene oxide GO
0.3 g graphite and 1.7 g potassium permanganate are joined in the 500 mL there-necked flasks with magneton, add the concentrated sulphuric acid and strong phosphoric acid mixed liquor that the volume ratio mixed in advance is 9:1, 18 h are reacted at 50 DEG C, after reaction terminates, sample is poured on the ice cube of 40 mL frozen in advance, 300 μ L are added under stirring slowly, massfraction is the hydrogen peroxide of 30%, react 30 min, centrifuging, and use the hydrochloric acid solution of 0.2 mol/L successively, ethanol washs three times, finally use ether centrifuge washing once, the solid obtained vacuum drying at 35 DEG C, obtain the pressed powder of brown color,
(2) preparation of gold nanoclusters AuNCsBSA
0.5 mmol gold chloride ethanolic solution and 0.5 mmol mercapto succinic acid MSA are mixed with 100 mL methyl alcohol by the method for logical nitrogen bubble, dropwise add 25 mL with vigorous stirring, the sodium borohydride solution of the fresh configuration of 0.2 mol/L, the dark-brown precipitation generated, centrifuging is also washed with water/ethanolic solution that volume ratio is 1:4, dry under vacuo, obtain MSAAuNCs solid;
MSAAuNCs and BSA is that the ratio of 1:10 adds in 5 mL water with mass ratio, stirs 5 min, stirs 7 hs at being 12,37 DEG C with the sodium hydrate regulator solution pH of 2 mol/L, and the product obtained is dialysed 24 h, and freeze drying obtains AuNCsBSA;
(3) two anti-label GO/AuNCsBSA/Ab
2the preparation of solution
Added in 2 mL ultrapure waters by the graphene oxide GO of 2 mg and the AuNCsBSA of 2 mg, vibrate 48 h, centrifugal and be re-dispersed into 1 mL, in the PBS buffer solution of pH7.4, adds 0.1 mg determinand antibody A b
2, at 4 DEG C, hatch 24 h, centrifugal and be re-dispersed into 1 mL, in the PBS buffer solution of pH 7.4, be stored in 4 DEG C for subsequent use.
embodiment 9two anti-label GO/AuNCsBSA/Ab
2the preparation of solution
(1) preparation of graphene oxide GO
0.4 g graphite and 2.1 g potassium permanganate are joined in the 500 mL there-necked flasks with magneton, add the concentrated sulphuric acid and strong phosphoric acid mixed liquor that the volume ratio mixed in advance is 9:1, 24 h are reacted at 70 DEG C, after reaction terminates, sample is poured on the ice cube of 50 mL frozen in advance, 400 μ L are added under stirring slowly, massfraction is the hydrogen peroxide of 30%, react 40 min, centrifuging, and use the hydrochloric acid solution of 0.2 mol/L successively, ethanol washs three times, finally use ether centrifuge washing once, the solid obtained vacuum drying at 35 DEG C, obtain the pressed powder of brown color,
(2) preparation of gold nanoclusters AuNCsBSA
0.8 mmol gold chloride ethanolic solution and 0.5 mmol mercapto succinic acid MSA are mixed with 100 mL methyl alcohol by the method for logical nitrogen bubble, dropwise add 30 mL with vigorous stirring, the sodium borohydride solution of the fresh configuration of 0.3 mol/L, the dark-brown precipitation generated, centrifuging is also washed with water/ethanolic solution that volume ratio is 1:4, dry under vacuo, obtain MSAAuNCs solid;
MSAAuNCs and BSA is that the ratio of 1:10 adds in 5 mL water with mass ratio, stirs 8 min, stirs 8 hs at being 12,37 DEG C with the sodium hydrate regulator solution pH of 3 mol/L, and the product obtained is dialysed 24 h, and freeze drying obtains AuNCsBSA;
(3) two anti-label GO/AuNCsBSA/Ab
2the preparation of solution
Added in 2 mL ultrapure waters by the graphene oxide GO of 3 mg and the AuNCsBSA of 2 mg, vibrate 72 h, centrifugal and be re-dispersed into 1 mL, in the PBS buffer solution of pH7.4, adds 0.15 mg determinand antibody A b
2, at 4 DEG C, hatch 32 h, centrifugal and be re-dispersed into 1 mL, in the PBS buffer solution of pH 7.4, be stored in 4 DEG C for subsequent use.
embodiment 10the detection of CEA
(1) three-electrode system of electrochemical workstation is used to test, Ag/AgCl electrode is as contrast electrode, platinum electrode is to electrode, prepared electrochemical luminous sensor is working electrode, electrochemical workstation and chemiluminescence detector are linked together the high pressure of photomultiplier is set to 800 V, cyclic voltammetry scan potential range is-1.8V ~-0.6V, and sweep speed is 0.1 V/s;
(2) containing in the PBS solution of 26 ~ 46 mmol/L potassium persulfates at 10 mL, pH 6.4 ~ 8.4, by electrochemical luminescence system, the electrochemical luminescence signals intensity that the CEA antigen of variable concentrations is produced is detected, drawing curve;
(3) detected instead of CEA antigen by testing sample solution, recording the range of linearity is 0.1 pg/mL ~ 5 ng/mL, detects and is limited to 0.03 pg/mL.
embodiment 11the detection of CA199
(1) three-electrode system of electrochemical workstation is used to test, Ag/AgCl electrode is as contrast electrode, platinum electrode is to electrode, prepared electrochemical luminous sensor is working electrode, electrochemical workstation and chemiluminescence detector are linked together the high pressure of photomultiplier is set to 800 V, cyclic voltammetry scan potential range is-1.8V ~-0.6V, and sweep speed is 0.1 V/s;
(2) containing in the PBS solution of 26 ~ 46 mmol/L potassium persulfates at 10 mL, pH 6.4 ~ 8.4, by electrochemical luminescence system, the electrochemical luminescence signals intensity that the CA199 antigen of variable concentrations is produced is detected, drawing curve;
(3) detected instead of CA199 antigen by testing sample solution, recording the range of linearity is 0.1 pg/mL ~ 5 ng/mL, detects and is limited to 0.03 pg/mL.
embodiment 12the detection of CA50
(1) three-electrode system of electrochemical workstation is used to test, Ag/AgCl electrode is as contrast electrode, platinum electrode is to electrode, prepared electrochemical luminous sensor is working electrode, electrochemical workstation and chemiluminescence detector are linked together the high pressure of photomultiplier is set to 800 V, cyclic voltammetry scan potential range is-1.8V ~-0.6V, and sweep speed is 0.1 V/s;
(2) containing in the PBS solution of 26 ~ 46 mmol/L potassium persulfates at 10 mL, pH 6.4 ~ 8.4, by electrochemical luminescence system, the electrochemical luminescence signals intensity that the CA50 antigen of variable concentrations is produced is detected, drawing curve;
(3) detected instead of CA50 antigen by testing sample solution, recording the range of linearity is 0.1 pg/mL ~ 5 ng/mL, detects and is limited to 0.03 pg/mL.
Claims (5)
1., based on a preparation method for the stomach cancer marker gold nanoclusters Electrochemiluminescsensor sensor of NP-NiGdAu, it is characterized in that, comprise the following steps:
(1) glass-carbon electrode of diameter 4 mm is taken turns doing polishing with 1.0 μm, 0.3 μm, 0.05 μm aluminum oxide polishing powder successively, clean with ultrapure water;
(2) painting 6 μ L, 0.5 ~ 2 mg mL is dripped
-1capture antibody base material NP-NiGdAu/Ab
1solution and electrode surface, 4 DEG C are saved to drying;
(3) drip 4 μ L, massfraction is the bovine serum albumin solution of 1 ~ 3%, with the nonspecific activity site on enclosed-electrode surface, rinse electrode surface by the PBS solution of pH6.4 ~ 8.4,4 DEG C are dried;
(4) drip the determinand antigen of 6 μ L variable concentrations, hatch 30 min at 4 DEG C, rinse electrode surface by the PBS solution of pH6.4 ~ 8.4,4 DEG C are dried;
(5) the anti-label GO/AuNCsBSA/Ab of painting 6 μ L bis-is dripped
2solution, rinse electrode surface by the PBS solution of pH6.4 ~ 8.4,4 DEG C are dried.
2. the preparation method of a kind of stomach cancer marker gold nanoclusters Electrochemiluminescsensor sensor based on NP-NiGdAu as claimed in claim 1, capture antibody base material NP-NiGdAu/Ab
1solution, is characterized in that, preparation process is as follows:
(1) preparation of NP-NiGdAu
NiGdAl alloy is put into the sodium hydroxide solution of 1.5 ~ 2.5 excessive mol/L, at room temperature chemical corrosion 32 ~ 74 h, centrifuge washing is to neutral, and in vacuum drying chamber, dry 12 ~ 48 h, obtain nanoporous NiGd;
By 80 ~ 120 mL, massfraction be 0.005 ~ 0.015% chlorauric acid solution boil, add 2 ~ 3 mL, massfraction is the trisodium citrate aqueous solution of 0.5 ~ 1.5%, add hot reflux 10 ~ 20 min, treat that solution colour becomes claret, solution is cooled to room temperature, the golden nanometer particle obtained, keeps in Dark Place at 4 DEG C;
The NP-NiGd of 1 ~ 3 mg is distributed in 1 mL ultrapure water, add 0.5 ~ 1.5 mL golden nanometer particle, the mixed solution obtained at room temperature vibrates 12 ~ 32 h, centrifuging, remove excessive golden nanometer particle, and product NP-NiGdAu is re-dispersed in 1 mL ultrapure water, obtained base material NP-NiGdAu solution;
(2) capture antibody base material NP-NiGdAu/Ab
1the preparation of solution
In base material NP-NiGdAu solution, add the determinand antibody A b of 10 ~ 100 μ L, 1 mg/mL
1solution, vibrate hatching 24 h at 4 DEG C, the determinand antibody A b that centrifugal removing is excessive
1, product is distributed in the PBS solution of 1 mL, pH 7.4, obtained capture antibody base material NP-NiGdAu/Ab
1solution, for subsequent use at being stored in 4 DEG C.
3. the preparation method of a kind of stomach cancer marker gold nanoclusters Electrochemiluminescsensor sensor based on NP-NiGdAu as claimed in claim 1, two anti-label GO/AuNCsBSA/Ab
2solution, is characterized in that, preparation process is as follows:
(1) preparation of graphene oxide GO
0.2 ~ 0.4 g graphite and 1.5 ~ 2.1 g potassium permanganate are joined in the 500 mL there-necked flasks with magneton, add the concentrated sulphuric acid and strong phosphoric acid mixed liquor that the volume ratio mixed in advance is 9:1, 6 ~ 24 h are reacted at 30 ~ 70 DEG C, after reaction terminates, sample is poured on the ice cube of 30 ~ 50 mL frozen in advance, 200 ~ 400 μ L are added under stirring slowly, massfraction is the hydrogen peroxide of 30%, reaction 20 ~ 40 min, centrifuging, and use the hydrochloric acid solution of 0.2 mol/L successively, ethanol washs three times, finally use ether centrifuge washing once, the solid obtained vacuum drying at 35 DEG C, obtain the pressed powder of brown color,
(2) preparation of gold nanoclusters AuNCsBSA
0.3 ~ 0.8 mmol gold chloride ethanolic solution and 0.5 mmol mercapto succinic acid MSA are mixed with 100 mL methyl alcohol by the method for logical nitrogen bubble, dropwise add 20 ~ 30 mL with vigorous stirring, the sodium borohydride solution of the fresh configuration of 0.1 ~ 0.3 mol/L, the dark-brown precipitation generated, centrifuging is also washed with water/ethanolic solution that volume ratio is 1:4, dry under vacuo, obtain MSAAuNCs solid;
MSAAuNCs and BSA is that the ratio of 1:10 adds in 5 mL water with mass ratio, stirs 3 ~ 8 min, stirring 6 ~ 8 h at being 12,37 DEG C with the sodium hydrate regulator solution pH of 1 ~ 3 mol/L, and the product obtained is dialysed 24 h, and freeze drying obtains AuNCsBSA;
(3) two anti-label GO/AuNCsBSA/Ab
2the preparation of solution
The graphene oxide GO of 1 ~ 3 mg and the AuNCsBSA of 2 mg is added in 2 mL ultrapure waters, vibration 32 ~ 72 h, centrifugal and be re-dispersed into 1 mL, in the PBS buffer solution of pH7.4, add 0.05 ~ 0.15 mg determinand antibody A b
2, at 4 DEG C, hatch 12 ~ 32 h, centrifugal and be re-dispersed into 1 mL, in the PBS buffer solution of pH 7.4, be stored in 4 DEG C for subsequent use.
4. a kind of preparation method of the stomach cancer marker gold nanoclusters Electrochemiluminescsensor sensor based on NP-NiGdAu for preparing of preparation method as claimed in claim 1, it is characterized in that, the electrochemical luminous sensor of preparation is used for the detection of determinand, and detecting step is as follows:
(1) three-electrode system of electrochemical workstation is used to test, Ag/AgCl electrode is as contrast electrode, platinum electrode is to electrode, prepared electrochemical luminous sensor is working electrode, electrochemical workstation and chemiluminescence detector are linked together the high pressure of photomultiplier is set to 800 V, cyclic voltammetry scan potential range is-1.8V ~-0.6V, and sweep speed is 0.1 V/s;
(2) containing in the PBS solution of 26 ~ 46 mmol/L potassium persulfates at 10 mL, pH 6.4 ~ 8.4, by electrochemical luminescence system, the electrochemical luminescence signals intensity that the determinand antigen of variable concentrations is produced is detected, drawing curve;
(3) determinand antigen is replaced by testing sample solution to detect.
5. the preparation method of a kind of stomach cancer marker gold nanoclusters Electrochemiluminescsensor sensor based on NP-NiGdAu as described in Claims 1 to 4, described determinand, is selected from one of following gastric cancer antigen: CEA, CA199, CA50, CA724, CA195, CA242, CYFRA2H.
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| CN114384053B (en) * | 2022-01-13 | 2023-09-08 | 山东大学 | Flash and glow adjustable near infrared chemiluminescence system with nano gold clusters as luminophors |
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