CN107589166B - One kind being based on TiO2It is situated between and sees the label-free type zearalenone optical electro-chemistry detection method and sensor of crystal - Google Patents
One kind being based on TiO2It is situated between and sees the label-free type zearalenone optical electro-chemistry detection method and sensor of crystal Download PDFInfo
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Abstract
The present invention discloses a kind of based on TiO2It is situated between and sees the label-free type zearalenone optical electro-chemistry detection method and sensor of crystal.This method utilizes gold cone (Au cone), the rutile TiO of polysulfide violet (PTH) sensitization2It is situated between and sees crystal (RTM) as photoelectric activity base material, and is used for immobilization zearalenone antibody (Ab)；The stability and good electric conductivity of surface plasma effect and polysulfide violet based on gold cone, the composite material of itself and RTM can accelerate the transfer velocity of light induced electron and improve photo-signal, work as zearalenone, that is the zearalenone antibody (Ab) of antigen and immobilization is immunoreacted, due to space steric effect, photosignal obviously weakens.Based on the phenomenon, the method established can be realized to zearalenone concentration 1 × 10‑6Highly sensitive detection within the scope of the ng/mL of ng/mL ~ 10.
Description
Technical field
The invention belongs to new function materials and bio-sensing detection technique field, and in particular to one kind is based on TiO2It is situated between and sees
The label-free type zearalenone optical electro-chemistry detection method and sensor of crystal.
Background technique
Zearalenone (Zearalenone) is also known as F-2 toxin, is the metabolite of Gibberella zeae bacterium, divides extensively
It is distributed in contaminated cereal and agricultural and sideline product, dairy produce, especially in corn and its fabricated product.Zearalenone has
Reproductive and developmental toxicity, immunotoxicity and strong Teratogenesis toxicity etc., can also impact endocrine, and may induce swollen
Tumor.Currently, the bioassay method of detection zearalenone mainly has enzyme-linked immunosorbent assay, electrochemistry and fluorescence spectrum
Deng.However, the sensitivity of these methods further enhances and explores a low background signal, favorable reproducibility, wide response
The sensing platform of range is still an urgent demand of zearalenone measurement.
Optical electro-chemistry (PEC) detection is the one kind set up based on photoelectrochemical process and chemical/biological identification process
New analysis method.This method, using photoelectric current as detection signal, has high sensitivity, response fast using light as excitation signal
The advantages that speed, equipment are simple and are easily miniaturized, providing one kind for fields such as clinical diagnosis, environmental monitoring and food safeties has by force
Power means.Photoelectrochemical assay uses light as excitation signal, and detection is electric signal, makees by using various forms of energy
For excitation signal and detection signal, makes to excite and detection signal is not interfere with each other, thus background signal is lower, can get higher spirit
Sensitivity.The realization of the type of photoelectric material, performance and photoelectrochemical process has direct and close relationship, photoelectric material itself
PhotoelectrochemicalProperties Properties, preparation method, composite effect, pattern control, charge-conduction rate etc. for the suitable of photoelectrochemical process
Benefit is realized and is had a major impact.
TiO2Nano material because of its unique photocatalytic activity, nontoxicity, excellent chemically and physically stability, make its at
For the ideal material of photocatalysis and optical electro-chemistry sensor.The performance of titanium dioxide is generally by crystal form, grain size, crystal face, knot
The influence of brilliant degree, specific surface area, micro-structure etc..TiO2The sight crystal that is situated between is that crystal subunit ordered arrangement is constituted, compared to biography
The TiO of system2Monocrystalline, TiO2It is situated between and sees crystal with more excellent solar energy conversion and catalytic performance, PEC performance can be significantly improved.
However, TiO2Forbidden bandwidth is larger, can only be therefore lower in visible region photoelectric conversion efficiency by ultraviolet excitation.
Summary of the invention
The TiO that the purpose of the present invention is be sensitized based on polysulfide violet2Be situated between the label-free type optical electro-chemistry sensor for seeing crystal
Prepare and be based on TiO2It is situated between and sees the label-free type zearalenone optical electro-chemistry detection method of crystal.
To realize that goal of the invention, one kind of the invention are based on TiO2It is situated between and sees the label-free type zearalenone photoelectricity of crystal
Chemical detection method adopts the following technical scheme that
(1) pretreatment of glass-carbon electrode (GCE): GCE mechanical grinding first on the chamois leather for be covered with alumina powder polishes,
Remained on surface powder is washed away with secondary water, then moves into ultrasonic water bath and cleans, until clean up, finally sequentially with ethyl alcohol, dilute
Acid and water thoroughly wash；
(2) rutile that 4 μ L concentration are 3mg/ml the preparation of PTH/Au cone/RTM/Ab/ZEN modified electrode: is added dropwise
Type TiO2Sight crystal (RTM) suspension that is situated between is dried under clean glassy carbon electrode surface, infrared lamp, is cooled to room temperature, then will
3.5 μ L gold bore solution drop coating to the electrode surface of modified, and Drying and cooling is to room temperature under infrared lamp；Electrode immersion is contained again
In the hac buffer of 4.8mmol/mL thionine pH=4.5, in the potential range of -0.4 ~ 1.2V, with the speed of 50Mv/s
Scan round 55 is enclosed, and realizes the modification of polysulfide violet (PTH), is taken out, is dried at room temperature for repairing to get to PTH/Au cone/RTM
Adorn electrode；Modified electrode is incubated for 50 under 37 °C in the zearalenone antibody A b solution that 5 μ L concentration are 1 mg/mL
Min then removes extra zearalenone antibody A b using the phosphate buffer solution of pH 7.5, then electrode is immersed 20 μ L
Concentration is 1 h of bovine serum albumin(BSA) (BSA) of 1.0 wt.%, and surface is washed away in nonspecific activity site on enclosed-electrode surface
After residual solution to get arrive PTH/Au cone/RTM/Ab modified electrode；Electrode is immersed to the zearalenone of 5 μ L various concentrations
25min is incubated under 37 °C in (ZEN, also referred to as Ag) standard solution；Electrode surface is rinsed simultaneously with the phosphate buffer solution of pH 7.5
Naturally dry at room temperature obtains PTH/Au cone/RTM/ Ab/ZEN modified electrode；
(3) detection of zearalenone: being measured using three-electrode system, with PTH/Au cone/RTM/Ab/ZEN
Modified electrode is working electrode, and Ag/AgCl is reference electrode, and platinum electrode is to be carried out to electrode using optical electro-chemistry work station
Detection, setting voltage are -0.1V, carry out switch lamp every 10s, the monochromatic light excitation light source of xenon lamp transmitting is using preceding by monochromator
Filtering；In the PBS buffer solution of pH 7.5, detection 1 × 10 is carried out by optical electro-chemistry work station-6 ng/mL~10 ng/mL
A series of zearalenone standard solution of various concentrations is drawn by the different current signals generated before and after record switch lamp
Working curve processed；Testing sample solution replaces zearalenone standard solution to be detected, and the result of detection can pass through work
Curve checks in.
Above-mentioned rutile TiO2Sight crystal (RTM) that is situated between is prepared by the following method:
0.5 g neopelex (SDBS) is dissolved in 25 mL, 2.2 mol/mL HNO3In solution, 15 points are stirred
Clock.Then 0.5 mL isopropyl titanate (IV) is added, 48 h are stirred under 80 °C.Then, products therefrom be centrifuged and with ultrapure water,
After ethanol washing 4 ~ 5 times, the dry 12h under 60 °C.It is remaining organic to remove that above-mentioned product calcines 1h in 400 °C of air
Rutile TiO is made in object2It is situated between and sees crystal.
It is of the present invention a kind of based on TiO2It is situated between and sees the label-free type zearalenone optical electro-chemistry sensor of crystal,
It is to electrode and Ag/ AgCl including working electrode, platinum electrode is reference electrode, which is characterized in that the working electrode is adopted
With PTH/Au cone/RTM/Ab/ZEN modified electrode, it is prepared by the method for following step: 1) polishing of glass-carbon electrode:
Glass-carbon electrode mechanical grinding first on the chamois leather for be covered with alumina powder polishes, and washes away remained on surface powder with secondary water, then
It moves into ultrasonic water bath and cleans, until cleaning up, finally sequentially thoroughly washed with ethyl alcohol, diluted acid and water；2) PTH/Au
The preparation of cone/RTM/Ab/ZEN modified electrode: 4 μ L concentration of dropwise addition are 3mg/mL rutile TiO2Being situated between, it is outstanding to see crystal (RTM)
Supernatant liquid is dried under clean glassy carbon electrode surface, infrared lamp, is cooled to room temperature, and 3.5 μ L gold are then bored solution drop coating to repairing
The electrode surface adornd, Drying and cooling is to room temperature under infrared lamp；Electrode is immersed again and contains 4.8mmol/mL thionine pH=4.5
Hac buffer in, in the potential range of -0.4 ~ 1.2V, with 55 circle of the speed loop of 50Mv/s scanning, take out,
It is dried at room temperature to get PTH/Au cone/RTM modified electrode is arrived；By modified electrode in the jade that 5 μ L concentration are 1 mg/mL
50 min are incubated under 37 °C in Zearlenone antibody A b solution, it is extra then to remove using the phosphate buffer solution of pH 7.5
Zearalenone antibody A b, then electrode is immersed into 1 h of BSA that 20 μ L concentration are 1.0 wt.%, on enclosed-electrode surface
Nonspecific activity site is washed away after surface residual liquid to get to PTH/Au cone/RTM/Ab modified electrode；Electrode is immersed
25min is incubated under 37 °C in zearalenone (ZEN, also referred to as Ag) standard solution of 5 μ L various concentrations；With pH's 7.5
Phosphate buffer solution rinses electrode surface and at room temperature naturally dry, obtains PTH/Au cone/RTM/ Ab/ZEN and repairs
Adorn electrode.
The invention has the characteristics that gold cone is used as carrier, it is possible to provide big surface area, high adsorption capacity and signal is put
Greatly.It is a kind of good electron mediator containing the thionine dyes molecule there are two amino, under acid condition, electropolymerization thionine list
Body molecule can form the porous poly- composite membrane of height (PTH).The compound membrane stability of this porous polysulfide violet (PTH) is good, not easily runs off, compares
Surface area is big, can be used as excellent fixation matrix, enhances the adsorbance of antibody, improves the sensitivity of immunosensor.The present invention
Pass through a simple one-step synthesis method rutile-type be situated between see crystal (RTM) and introduce a typical sensitizer polysulfide violet come into
The photoelectric conversion efficiency of one step raising RTM.Antigen forms space steric effect, will significantly reduce in conjunction with antibody specificity
Photo-signal.Photo-signal and zearalenone concentration are linear in a certain range, realize zearalenone
Highly sensitive detection.
Remarkable advantage of the invention are as follows:
(1) rutile TiO being sensitized with polysulfide violet2The sight crystal that is situated between is photoelectric activity base material, rutile TiO2It is situated between
Crystal porosity with higher is seen, the introducing of poly-dopamine enhances the transmission speed of electronics, with individual conventional Ti O2It is single
Brilliant material is compared, and the sensitivity of photoelectric conversion efficiency and sensor is effectively improved.
(2) antigen forms space steric effect in conjunction with antibody specificity, and sensor obtained is realized to Gibberella zeae
The super sensitivity detection of ketenes.
Detailed description of the invention
Fig. 1 is of the present invention based on TiO2It is situated between and sees the preparation process schematic diagram of the optical electro-chemistry sensor of crystal.
The SEM that Fig. 2A is RTM schemes.
The TEM figure and SEAD figure that Fig. 2 B is RTM.
Fig. 3 A is the impedance diagram of electrode structure.
Fig. 3 B is electrode structure photocurrent response figure.
Fig. 4 is in various concentration 1 × 10-6The ng/mL(a-h of ng/mL ~ 10) zearalenone standard solution the case where
Under, the photocurrent response figure of sensing electrode.
Specific embodiment
The present invention is further illustrated by the following examples, but protection scope of the present invention is not limited to following reality
Apply example.
Embodiment 1
One kind being based on TiO2It is situated between and sees the preparation method (as shown in Figure 1) of the label-free type optical electro-chemistry sensor of crystal:
(1) pretreatment of glass-carbon electrode: glass-carbon electrode mechanical grinding first on the chamois leather for be covered with alumina powder polishes,
Remained on surface powder is washed away with secondary water, then moves into ultrasonic water bath and cleans, until clean up, finally sequentially with ethyl alcohol, dilute
Acid and water thoroughly wash；
(2) rutile TiO prepared by 2 method of embodiment that 4 μ L concentration are 3 mg/ml is added dropwise2Being situated between, it is outstanding to see crystal (RTM)
Supernatant liquid is dried under clean glassy carbon electrode surface, infrared lamp, is cooled to room temperature；
(3) 3.5 μ L gold are bored into solution drop coating to the electrode surface of modified, Drying and cooling is to room temperature under infrared lamp；It is real
The cone solution of nanogold used in testing provides for Foochow Jie Sheng nano material Co., Ltd.
(4) electrode is immersed in the hac buffer containing 4.8mmol/mL thionine pH=4.5 again, in -0.4 ~ 1.2V
Potential range in, with 55 circle of the speed loop of 50Mv/s scanning, realize the modification of polysulfide violet (PTH).It then takes out, in room
Temperature is dried to arrive PTH/Au cone/RTM modified electrode down；Modified electrode is red in the corn that 5 μ L concentration are 1 mg/mL
50 min are incubated under 37 °C in mould ketenes antibody A b solution, then remove extra corn using the phosphate buffer solution of pH 7.5
Zeranol antibody A b, then electrode is immersed into 1 h of bovine serum albumin(BSA) (BSA) that 20 μ L concentration are 1.0 wt.%, enclosed-electrode
Nonspecific activity site on surface is washed away after surface residual liquid to get to PTH/Au cone/RTM/Ab modified electrode.
(5) electrode is immersed in zearalenone (ZEN, also referred to as Ag) standard solution of 5 μ L various concentrations under 37 °C
It is incubated for 25min；Electrode surface and at room temperature naturally dry are rinsed with the phosphate buffer solution of pH 7.5, obtains PTH/Au
Cone/RTM/ Ab/ZEN modified electrode；Impedance and the photocurrent response for testing electrode, respectively as shown in Fig. 3 A and Fig. 3 B.
Embodiment 2
Rutile TiO2It is situated between and sees the preparation of crystal (RTM):
0.5 g neopelex (SDBS) is dissolved in 25 mL, 2.2 mol/ml HNO3In solution, 15 points are stirred
Clock.Then 0.5 mL isopropyl titanate (IV) is added, 48 h are stirred under 80 °C.Then, products therefrom is centrifuged and with ultrapure
Water, after ethanol washing 4 ~ 5 times, the dry 12h under 60 °C.It is remaining to remove that above-mentioned product calcines 1h in 400 °C of air
Rutile TiO is made in organic matter2It is situated between and sees crystal.Electron emission scanning electron microscope (SEM) figure of RTM, such as Fig. 2A institute
Show, RTAM is the porous structure of 90 ~ 130 nm sizes.Transmission electron microscope (TEM) figure and selective electron diffraction (SAED) figure of RTM,
As shown in Figure 2 B, show rutile TiO2The formation of mesomorphic.
Embodiment 3
One kind being based on TiO2It is situated between and sees detection side of the label-free optical electro-chemistry sensor for zearalenone of crystal
Method, steps are as follows:
(1) it is measured using three-electrode system, using PTH/Au cone/RTM/Ab/ZEN modified electrode as working electrode,
Ag/AgCl is reference electrode, and platinum electrode is to be detected to electrode using optical electro-chemistry work station, setting voltage is-
0.1V carries out switch lamp every 10s, and the monochromatic light excitation light source of xenon lamp transmitting is filtered using preceding by monochromator；
(2) in the PBS buffer solution of pH 7.5, detection 1 × 10 is carried out by optical electro-chemistry work station-6 ng/mL~10
A series of zearalenone standard solution of various concentrations of ng/mL is believed by the different electric currents generated before and after record switch lamp
Number, working curve is drawn, Fig. 4 is the photocurrent response of sensing electrode and the linear relationship of zearalenone concentration of standard solution
Figure；
(3) zearalenone standard solution is replaced to detect testing sample solution, the result of detection can pass through work
It is checked in as curve.
In this experiment, gold cone is used as carrier, it is possible to provide big surface area, high adsorption capacity and signal amplification.Contain
Thionine dyes molecule there are two amino is a kind of good electron mediator, under acid condition, electropolymerization thionine monomer molecule
The porous poly- composite membrane of height can be formed.This porous compound membrane stability of polysulfide violet is good, not easily runs off, large specific surface area, can be used as
Excellent fixation matrix, enhances the adsorbance of antibody, improves the sensitivity of immunosensor.The present invention passes through one simple one
Footwork titania type, which is situated between, to be seen crystal (RTM) and introduces a typical sensitizer polysulfide violet to further increase the light of RTM
Photoelectric transformation efficiency.Antigen forms space steric effect, will significantly reduce photo-signal in conjunction with antibody specificity.Photoelectricity
It flows signal and zearalenone concentration is linear in a certain range, realize the highly sensitive detection of zearalenone.
Claims (3)
1. one kind is based on TiO2It is situated between and sees the label-free type zearalenone optical electro-chemistry detection method of crystal, which is characterized in that packet
Include following steps:
1) pretreatment of glass-carbon electrode: glass-carbon electrode mechanical grinding first on the chamois leather for be covered with alumina powder polishes, with two
Remained on surface powder is removed in secondary washing, then moves into ultrasonic water bath and clean, until clean up, finally sequentially with ethyl alcohol, diluted acid and
Water thoroughly washs；
2) polysulfide violet/gold cone/rutile TiO2It is situated between and sees crystal/zearalenone antibody/zearalenone modified electrode
Preparation: the rutile TiO that 4 μ L concentration are 3mg/ml is added dropwise2Jie's sight Crystal suspensions are infrared in clean glassy carbon electrode surface
It dries, is cooled to room temperature under lamp, then dry 3.5 μ L gold cone solution drop coating under infrared lamp to the electrode surface of modified
It is cooled to room temperature；Electrode is immersed again in the hac buffer of pH=4.5 containing 4.8mmol/mL thionine, in -0.4 ~ 1.2V
Potential range in, with 55 circle of the speed loop of 50Mv/s scanning, realize the modification of polysulfide violet；It then takes out, does at room temperature
It is dry to get arrive polysulfide violet/gold cone/rutile TiO2It is situated between and sees crystal modified electrode；By this modified electrode in 5 μ L concentration be 1
50 min are incubated under 37 °C in the zearalenone antibody-solutions of mg/mL, are then gone using the phosphate buffer solution of pH 7.5
1 h of bovine serum albumin(BSA) that 20 μ L concentration are 1.0 wt.%, envelope are immersed except extra zearalenone antibody, then by electrode
Nonspecific activity site on electrode surface is closed, is washed away after surface residual liquid to get to polysulfide violet/gold cone/rutile TiO2
It is situated between and sees crystal/zearalenone antibody modification electrode；The zearalenone standard that this electrode is immersed 5 μ L various concentrations is molten
25min is incubated under 37 °C in liquid；Electrode surface is rinsed with the phosphate buffer solution of pH 7.5 and is dried in the air naturally at room temperature
It is dry, obtain polysulfide violet/gold cone/rutile TiO2It is situated between and sees crystal/zearalenone antibody/zearalenone modification electricity
Pole；
3) detection of zearalenone: being measured using three-electrode system, with polysulfide violet/gold cone/rutile TiO2It is situated between and sees
Crystal/zearalenone antibody/zearalenone modified electrode is working electrode, and Ag/AgCl is reference electrode, platinum filament electricity
It extremely to electrode, is detected using optical electro-chemistry work station, setting voltage is -0.1V, carries out switch lamp, xenon lamp every 10s
The monochromatic light excitation light source of transmitting is filtered using preceding by monochromator；In the PBS buffer solution of pH 7.5, pass through optical electro-chemistry
Work station carries out detection 1 × 10-6A series of zearalenone standard solution of various concentrations of the ng/mL of ng/mL ~ 10, passes through
The different current signals generated before and after switch lamp are recorded, working curve is drawn；Testing sample solution replaces zearalenone mark
Quasi- solution is detected, and the result of detection can be checked in by working curve.
2. according to claim 1 be based on TiO2It is situated between and sees the label-free type zearalenone optical electro-chemistry detection side of crystal
Method, which is characterized in that the rutile TiO2The sight crystal that is situated between is prepared by the following method: 0.5 g neopelex
It is dissolved in 25 mL, 2.2 mol/mL HNO3In solution, stir 15 minutes；Then 0.5mL isopropyl titanate is added, is stirred under 80 °C
It mixes 48 h and obtains crude product in solution；Then, gained crude product in solution is centrifuged and with after ultrapure water, ethanol washing 4 ~ 5 time, at 60 °C
Lower dry 12h purified product；Above-mentioned purified product calcines 1h in 400 °C of air to remove remaining organic matter, and golden red is made
Stone-type TiO2It is situated between and sees crystal.
3. one kind is based on TiO2It is situated between and sees the label-free type zearalenone optical electro-chemistry sensor of crystal, including working electrode, platinum
It is reference electrode that silk electrode, which is to electrode and Ag/ AgCl, which is characterized in that the working electrode is using polysulfide violet/gold cone gold
Red stone-type TiO2It is situated between and sees crystal/zearalenone antibody/zearalenone modified electrode, by the method system of following step
It is standby to form:
1) polishing of glass-carbon electrode: glass-carbon electrode mechanical grinding first on the chamois leather for be covered with alumina powder polishes, and use is secondary
Remained on surface powder is removed in washing, then moves into ultrasonic water bath and clean, until cleaning up, finally sequentially uses ethyl alcohol, diluted acid and water
Thoroughly washing；
2) polysulfide violet/gold cone/rutile TiO2It is situated between and sees crystal/zearalenone antibody/zearalenone modified electrode
Preparation: 4 μ L concentration of dropwise addition are 3mg/mL rutile TiO2Jie's sight Crystal suspensions are infrared in clean glassy carbon electrode surface
It dries, is cooled to room temperature under lamp, then dry 3.5 μ L gold cone solution drop coating under infrared lamp to the electrode surface of modified
It is cooled to room temperature；Electrode is immersed again in the hac buffer of pH=4.5 containing 4.8mmol/mL thionine, in -0.4 ~ 1.2V
Potential range in, with 55 circle of the speed loop of 50Mv/s scanning, take out, be dried at room temperature for get bored to polysulfide violet/gold/
Rutile TiO2It is situated between and sees crystal modified electrode；This modified electrode is resisted in the zearalenone that 5 μ L concentration are 1 mg/mL
50 min are incubated in liquid solution under 37 °C, then remove extra zearalenone using the phosphate buffer solution of pH 7.5
Antibody, then this electrode is immersed into 1 h of bovine serum albumin(BSA) that 20 μ L concentration are 1.0 wt.%, it is non-specific on enclosed-electrode surface
Property active site, wash away after surface residual liquid to get to polysulfide violet/gold cone/rutile TiO2It is situated between and sees crystal/Gibberella zeae alkene
Ketone antibody modification electrode；This electrode is immersed in the zearalenone standard solution of 5 μ L various concentrations and is incubated for 25 under 37 °C
min；Rinse electrode surface and at room temperature naturally dry with the phosphate buffer solution of pH 7.5, obtain polysulfide violet/gold bore/
Rutile TiO2It is situated between and sees crystal/zearalenone antibody/zearalenone modified electrode.
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| CN108802015B (en) * | 2018-06-13 | 2020-08-21 | 福建省妇幼保健院 | Preparation of zearalenone electrochemiluminescence sensor based on peptide sensor |
| CN109932407B (en) * | 2019-04-10 | 2021-01-29 | 福建省妇幼保健院 | Sandwich type prostate specific antigen photoelectrochemical detection method based on in-situ signal amplification |
| CN109975375B (en) * | 2019-04-11 | 2021-07-02 | 福建师范大学 | Zearalenone detection method based on signal enhancement type polymer functionalized rutile phase titanium dioxide mesocrystal |
| CN111077201B (en) * | 2019-12-23 | 2022-03-22 | 江苏大学 | Preparation method of photoelectrochemical aptamer sensor for monitoring zearalenone |
| CN112505115A (en) * | 2020-12-17 | 2021-03-16 | 东北农业大学 | Preparation and detection method of three-dimensional photosensitive electrode for detecting phospholipids in crude oil |
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