CN108866216A - It is a kind of for identifying the primer combination of probe of mycobacterium abscessus and Marseille mycobacteria - Google Patents
It is a kind of for identifying the primer combination of probe of mycobacterium abscessus and Marseille mycobacteria Download PDFInfo
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- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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- C12Q1/686—Polymerase chain reaction [PCR]
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Abstract
The invention discloses a kind of primer combination of probe for identifying mycobacterium abscessus and Marseille mycobacteria.Single stranded DNA shown in sequence 1- sequence 4 of the primer combination of probe provided by the invention by sequence table forms.The present invention provides a kind of mycobacterium abscessus and Marseille mycobacteria identification method, the differentiation to mycobacterium abscessus and Marseille mycobacteria is rapidly completed from molecular level, have the characteristics that it is easy, quickly, high specific and high sensitivity, thus solve existing molecular engineering can not accurate, Rapid identification mycobacterium abscessus compound group the characteristics of.
Description
Technical field
The present invention relates to a kind of primer combination of probe for identifying mycobacterium abscessus and Marseille mycobacteria.
Background technique
Non-tuberculous mycobacteria (nontuberculous mycobacteria, NTM) refer to remove and Mycobacterium leprae with
Outer all mycobacteria strains.NTM is widely present in the water in natural environment, soil and aerosol, most of for rotten object
Bacterial parasite, only small part is to human body cause illness.Epidemiological analysis in recent years shows that the disease incidence of global NTM disease is in increase
Gesture, be more even previously considered to be non-pathogenic NTM, also have pathogenic report.The all previous tuberculosis epidemiology in China
Survey data shows, NTM separation rate from the 4.9% of nineteen ninety rise to 2000 11.1%, be further increased within 2010
22.9%, which implies that China NTM is in the situation obviously risen.The reason of increasing for NTM disease disease incidence, may with it is immune
The low crowd of power increases, environmental exposure increases and aging of population is related, how to face the new challenge of mycobacterial diseases, is
The severe project that the mankind face at present.Most common in all pathogenic NTM is to belong to the compound group of mycobacterium abscessus and bird is intracellular
The compound group of mycobacteria.
The compound group of mycobacterium abscessus is also mostly invalid to common antibacterials to a line antituberculotic natural drug resistance,
Therapeutic scheme is usually using macrolide antibiotics such as clarithromycin as the multiple medicine scheme for combining of core, and cure rate is only about
60%, therefore be the key points and difficulties that NTM disease is treatment.The pathogenic compound group of mycobacterium abscessus mainly includes abscess branch bar
Bacterium and Marseille mycobacteria, although showing very strong similitude in terms of biology, two kinds of strains to clarithromycin not
With resistant characterization, therefore, early stage completes the drug resistance analysis of identification and clarithromycin to two kinds of mycobacterias for improving
The cure rate of non-tuberculous mycobacteria is significant.
Summary of the invention
The object of the present invention is to provide a kind of primer combination of probe for identifying mycobacterium abscessus and Marseille mycobacteria.
The present invention provides primer combination of probe, are made of primers F 1, primer R1, probe P1 and probe P2；
The primers F 1 is following (a1) or (a2)：
(a1) single strand dna shown in the sequence 1 of sequence table；
(a2) sequence 1 is had by the substitution and/or deletion and/or addition of one or several nucleotide and with sequence 1
The DNA molecular of identical function；
The primer R1 is following (a3) or (a4)：
(a3) single strand dna shown in the sequence 2 of sequence table；
(a4) sequence 2 is had by the substitution and/or deletion and/or addition of one or several nucleotide and with sequence 2
The DNA molecular of identical function；
The probe P1 is following (a5) or (a6)：
(a5) single strand dna shown in the sequence 3 of sequence table；
(a6) sequence 3 is had by the substitution and/or deletion and/or addition of one or several nucleotide and with sequence 3
The DNA molecular of identical function；
The probe P2 is following (a7) or (a8)：
(a7) single strand dna shown in the sequence 4 of sequence table；
(a8) sequence 4 is had by the substitution and/or deletion and/or addition of one or several nucleotide and with sequence 4
The DNA molecular of identical function.
The probe P1 is modified by fluorophor first and quenching group；
The probe P2 is modified by fluorophor second and quenching group；
Fluorophor first and fluorophor second are different fluorophors.
Fluorophor first is FAM fluorophor；
Fluorophor second is HEX fluorophor.
The quenching group is BHQ1 quenching group.
The purposes of any description above primer combination of probe is any one of following (b1)-(b4):
(b1) identify mycobacterium abscessus and Marseille mycobacteria；
(b2) it detects in sample to be tested and whether contains mycobacterium abscessus or Marseille mycobacteria；
(b3) kit for identifying mycobacterium abscessus and Marseille mycobacteria is prepared；
(b4) preparation for detect in sample to be tested whether the kit containing mycobacterium abscessus or Marseille mycobacteria.
The present invention also protects the application of the primer combination of probe, for any one of following (b1)-(b4):
(b1) identify mycobacterium abscessus and Marseille mycobacteria；
(b2) it detects in sample to be tested and whether contains mycobacterium abscessus or Marseille mycobacteria；
(b3) kit for identifying mycobacterium abscessus and Marseille mycobacteria is prepared；
(b4) preparation for detect in sample to be tested whether the kit containing mycobacterium abscessus or Marseille mycobacteria.
The present invention also protects the kit containing the primer combination of probe；The purposes of the kit be following (c1) or
(c2)：
(c1) identify mycobacterium abscessus and Marseille mycobacteria；
(c2) it detects in sample to be tested and whether contains mycobacterium abscessus or Marseille mycobacteria.
The present invention also protects the preparation method of the kit, includes the steps that individually packing each primed probe.
The present invention also protects the system containing any description above primer combination of probe or the kit；The system
Purposes is following (c1) or (c2)：
(c1) identify mycobacterium abscessus and Marseille mycobacteria；
(c2) it detects in sample to be tested and whether contains mycobacterium abscessus or Marseille mycobacteria.
The system also includes identify instrument required for mycobacterium abscessus and Marseille mycobacteria using quantitative PCR.
The present invention also protects a kind of method for identifying mycobacterium abscessus and Marseille mycobacteria, includes the following steps：With
The genomic DNA of strain to be tested is template, quantitative fluorescent PCR is carried out using the primer combination of probe, if fluorophor first
Channel shows positive findings and fluorophor second channel shows positive findings, and strain to be tested is mycobacterium abscessus, if fluorescence
Group first channel shows positive findings and fluorophor second channel shows that negative findings, strain to be tested are Marseille mycobacteria.
The present invention also protect in a kind of detection sample to be tested whether the side containing mycobacterium abscessus or Marseille mycobacteria
Method includes the following steps：Using the total DNA of sample to be tested as template, carried out using the primer combination of probe of any description above glimmering
Fluorescent Quantitative PCR, if fluorophor first channel shows positive findings and fluorophor second channel shows positive findings, sample to be tested
In in contain mycobacterium abscessus；If fluorophor first channel shows positive findings and the negative knot of fluorophor second channel display
Fruit, in sample to be tested in contain Marseille mycobacteria；If fluorophor first channel shows negative findings and fluorophor second is logical
Road shows negative findings, and Marseille mycobacteria is not contained in sample to be tested and does not contain mycobacterium abscessus.
Any description above positive findings concretely value >=36 Ct (or undet)；
Any description above negative findings concretely Ct value<36.
Any description above quantitative fluorescent PCR reaction system is concretely：Primers F 1, primer R1, probe P1, probe P2 are each
1 μ l, PCR buffer+dNTPs+Mg2+(PCR mix) 25 μ l, 4 μ l, RNase Free H of DNA profiling2O12μl.Each primer exists
Concentration in system is 500nM, and concentration of each probe in system is 250nM.DNA adds to reaction in the form of DNA solution
In system, the concentration of DNA is 10ng/ μ l in DNA solution.
Any description above quantitative fluorescent PCR response procedures are concretely：95 DEG C of initial denaturation 2min, 95 DEG C are denaturalized 5 seconds, and 58
DEG C annealing 20 seconds, 72 DEG C extend 20 seconds, totally 40 circulation.
Any description above strain to be tested concretely mycobacterium abscessus, mycobacterium avium, Mycobacterium intracellulare, bear Sa
This mycobacteria or Marseille mycobacteria reference culture.
The present invention provides a kind of mycobacterium abscessus and Marseille mycobacteria identification method, is rapidly completed pair from molecular level
The differentiation of mycobacterium abscessus and Marseille mycobacteria has the characteristics that easy, quick, high specific and high sensitivity, thus
Solve existing molecular engineering can not accurate, Rapid identification mycobacterium abscessus compound group the characteristics of.
Detailed description of the invention
Fig. 1 is to contain Marseille mycobacteria amplification figure in sample, the channel FAM amplification curve only occurs.
Fig. 2 is to contain mycobacterium abscessus amplification figure in sample, the channel FAM and HEX amplification curve occurs.
Specific embodiment
Embodiment below facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments
Method is unless otherwise specified conventional method.Test material as used in the following examples is unless otherwise specified certainly
What routine biochemistry reagent shop was commercially available.Quantitative test in following embodiment is respectively provided with three repeated experiments, as a result makes even
Mean value.
Embodiment 1, the design of primer combination of probe
A large amount of sequence analyses are carried out to mycobacterium abscessus and Marseille mycobacteria, are compared, and to mycobacterium abscessus and
The influence factor of Marseille mycobacteria clarithromycin pharmaceutical sensibility is analyzed, and is obtained a set of for identifying abscess branch bar
The primer combination of probe of bacterium and Marseille mycobacteria, as follows：
Primers F 1 (sequence 1 of sequence table)：5'-GCGACGCCAGTGGGGCTGGT-3'；
Primer R1 (sequence 2 of sequence table)：5'-CGCCGCCTGATCACCAGCAC-3'；
Probe P1 (sequence 3 of sequence table)：5'-GCGGATCGTCGCCGAATCCGGT-3'
Probe P2 (sequence 4 of sequence table) 5 '-CGCCCTACCAAGTCACCAGCG-3 '.
5 ' the ends of probe P1 are modified using FAM fluorophor, and 3 ' ends are modified using BHQ1 quenching group.
5 ' the ends of probe P2 are modified using HEX fluorophor, and 3 ' ends are modified using BHQ1 quenching group.
Probe P1 can be detected simultaneously by mycobacterium abscessus and Marseille mycobacteria, and probe P2 is only capable of detecting abscess
Mycobacteria.
The foundation of embodiment 2, detection method
1, the total DNA of sample to be tested is extracted.
2, the total DNA obtained using step 1 carries out fluorescence quantitative PCR detection as template.
Quantitative fluorescent PCR reaction system：Each 1 μ l, PCR buffer+dNTPs+ of primers F 1, primer R1, probe P1, probe P2
Mg2+(PCR mix) 25 μ l, 4 μ l, RNase Free H of DNA profiling2O 12μl。
Concentration of each primer in system is 500nM, and concentration of each probe in system is 250nM.
DNA is added in reaction system in the form of DNA solution, and the concentration of DNA is 10ng/ μ l in DNA solution.
Quantitative fluorescent PCR response procedures：95 DEG C of initial denaturation 2min, 95 DEG C are denaturalized 5 seconds, and 58 DEG C are annealed 20 seconds, 72 DEG C of extensions
20 seconds, totally 40 recycled.
Positive controls and negative control group are set simultaneously.
Mycobacterium abscessus reference culture (ATCC19977) and Marseille mycobacteria standard bacteria is respectively adopted in positive controls
The genomic DNA of strain (CIP108297 is purchased from Pasteur Institut) is as template (DNA concentration 106Copy/ml).
Negative control group is using deionized water negative controls as template.
3, after completing step 2, testing result is analyzed as follows：
If value >=36 Ct (or undet), result is feminine gender；If Ct value<36, result is the positive.
Negative control Ct value answers >=36, and positive control Ct value is answered<25.
When FAM signal is the positive, while HEX signal is positive, there are mycobacterium abscessus (Fig. 2) in sample to be tested；
When FAM signal is the positive, while HEX signal is negative, there are Marseille mycobacteria (Fig. 1) in sample to be tested；
When FAM signal is feminine gender, while HEX signal is negative, in sample to be tested there is no Marseille mycobacteria and not
There are mycobacterium abscessus.
Because two kinds of bacterium of clinic can not exist simultaneously, therefore the above method can be used to detect and identify the abscess in sample point
Branch bacillus and Marseille mycobacteria.
Embodiment 3, reference culture detection
Strain to be tested：Mycobacterium abscessus (ATCC19977), mycobacterium avium (ATCC25291), Mycobacterium intracellulare
(ATCC13950), (CIP108297 is purchased from method for mycobacterium kansasii (ATCC12478) and Marseille mycobacteria reference culture
State's Institute Pasteur).
It is detected using the method in embodiment 2, the results showed that, sample to be tested is mycobacterium avium, branch bar intracellular
When bacterium and mycobacterium kansasii, FAM signal is feminine gender, while HEX signal is feminine gender；When sample to be tested is mycobacterium abscessus
When, FAM signal is the positive, while HEX signal is the positive；When sample to be tested is Marseille mycobacteria reference culture, FAM signal
For the positive, while HEX signal is feminine gender.
The above method has good specificity.
Embodiment 4, actual sample detection
Sample to be tested is：50 parts of mycobacterium abscessus, 30 parts of Marseille mycobacterias, 10 parts of mycobacterium aviums, 10 points it is intracellular
Mycobacteria, 10 parts of mycobacterium kansasiis and 10 parts of mycobacterium tuberculosis.
Sample to be tested is disclosed in following document：In Vitro Activity of Clofazimine against Non-
tuberculous Mycobacteriaisolated in Beijing,China.Luo J,Yu X,Jiang G,Fu Y,Huo
F,Ma Y,Wang F,Shang Y,Liang Q,Xue Y,Huang H.Antimicrob Agents Chemother.2018
May 14.pii:AAC.00072-18.doi:10.1128/AAC.00072-18.；The public can be attached from the Capital University of Medical Sciences
BJ Chest Science Hospital obtains.
It is detected using the method in embodiment 2, the results are shown in Table 1.
1 testing result of table
Using method detection mycobacterium abscessus of the invention and Marseille mycobacteria, as a result accurately the above results show
Reliably, specificity is good.
Embodiment 5, minimum detectability detection
Strain to be tested：(CIP108297 is purchased from for mycobacterium abscessus (ATCC19977) and Marseille mycobacteria reference culture
Pasteur Institut).
The genomic DNA of strain to be tested is extracted, and doubling dilution is carried out to it, is detected with the method in embodiment 2,
The results are shown in Table 2.
2 testing result of table
The above results show the above method for DNA copy number in sample 102Sample have good sensitivity,
It can correctly detect mycobacterium abscessus and Marseille mycobacteria.
Sequence table
<110>Attached BJ Chest Science Hospital of the Capital University of Medical Sciences
Beijing Tuberculosis and Thoracic Tumor Research Institute
<120>It is a kind of for identifying the primer combination of probe of mycobacterium abscessus and Marseille mycobacteria
<160> 4
<170> SIPOSequenceListing 1.0
<210> 1
<211> 20
<212> DNA
<213>Artificial sequence (Artificial Sequence)
<400> 1
gcgacgccag tggggctggt 20
<210> 2
<211> 20
<212> DNA
<213>Artificial sequence (Artificial Sequence)
<400> 2
cgccgcctga tcaccagcac 20
<210> 3
<211> 22
<212> DNA
<213>Artificial sequence (Artificial Sequence)
<400> 3
gcggatcgtc gccgaatccg gt 22
<210> 4
<211> 21
<212> DNA
<213>Artificial sequence (Artificial Sequence)
<400> 4
cgccctacca agtcaccagc g 21
Claims (10)
1. primer combination of probe is made of primers F 1, primer R1, probe P1 and probe P2；
The primers F 1 is following (a1) or (a2)：
(a1) single strand dna shown in the sequence 1 of sequence table；
(a2) have by sequence 1 by the substitution and/or deletion and/or addition of one or several nucleotide and with sequence 1 identical
The DNA molecular of function；
The primer R1 is following (a3) or (a4)：
(a3) single strand dna shown in the sequence 2 of sequence table；
(a4) have by sequence 2 by the substitution and/or deletion and/or addition of one or several nucleotide and with sequence 2 identical
The DNA molecular of function；
The probe P1 is following (a5) or (a6)：
(a5) single strand dna shown in the sequence 3 of sequence table；
(a6) have by sequence 3 by the substitution and/or deletion and/or addition of one or several nucleotide and with sequence 3 identical
The DNA molecular of function；
The probe P2 is following (a7) or (a8)：
(a7) single strand dna shown in the sequence 4 of sequence table；
(a8) have by sequence 4 by the substitution and/or deletion and/or addition of one or several nucleotide and with sequence 4 identical
The DNA molecular of function.
2. primer combination of probe as described in claim 1, it is characterised in that：
The probe P1 is modified by fluorophor first and quenching group；
The probe P2 is modified by fluorophor second and quenching group；
Fluorophor first and fluorophor second are different fluorophors.
3. primer combination of probe as claimed in claim 2, it is characterised in that：
Fluorophor first is FAM fluorophor；
Fluorophor second is HEX fluorophor.
4. the application of any primer combination of probe of claims 1 to 3, for any one of following (b1)-(b4):
(b1) identify mycobacterium abscessus and Marseille mycobacteria；
(b2) it detects in sample to be tested and whether contains mycobacterium abscessus or Marseille mycobacteria；
(b3) kit for identifying mycobacterium abscessus and Marseille mycobacteria is prepared；
(b4) preparation for detect in sample to be tested whether the kit containing mycobacterium abscessus or Marseille mycobacteria.
5. the kit containing any primer combination of probe of claims 1 to 3；The purposes of the kit is as follows
(c1) or (c2)：
(c1) identify mycobacterium abscessus and Marseille mycobacteria；
(c2) it detects in sample to be tested and whether contains mycobacterium abscessus or Marseille mycobacteria.
6. the preparation method of kit described in claim 5 includes the steps that individually packing each primed probe.
7. the system containing kit described in the claims 1 to 3 any primer combination of probe or claim 5；Institute
The purposes for stating system is following (c1) or (c2)：
(c1) identify mycobacterium abscessus and Marseille mycobacteria；
(c2) it detects in sample to be tested and whether contains mycobacterium abscessus or Marseille mycobacteria.
8. system as claimed in claim 7, it is characterised in that：The system also includes identify abscess branch using quantitative PCR
Instrument required for bacillus and Marseille mycobacteria.
9. a kind of method for identifying mycobacterium abscessus and Marseille mycobacteria, includes the following steps：With the gene of strain to be tested
Group DNA is template, quantitative fluorescent PCR is carried out using any primer combination of probe of Claims 2 or 3, if fluorescent base
The channel Tuan Jia shows positive findings and fluorophor second channel shows positive findings, and strain to be tested is mycobacterium abscessus, if
Fluorophor first channel shows positive findings and fluorophor second channel shows that negative findings, strain to be tested are Marseille branch bar
Bacterium.
10. in a kind of detection sample to be tested whether the method containing mycobacterium abscessus or Marseille mycobacteria, including walk as follows
Suddenly：Using the total DNA of sample to be tested as template, fluorescent quantitation is carried out using any primer combination of probe of Claims 2 or 3
PCR, if fluorophor first channel shows positive findings and fluorophor second channel and shows positive findings, in sample to be tested in contain
There is mycobacterium abscessus；If fluorophor first channel shows positive findings and fluorophor second channel shows negative findings, to
Contain Marseille mycobacteria in test sample sheet；If fluorophor first channel shows negative findings and fluorophor second channel is shown
Negative findings without containing Marseille mycobacteria and do not contain mycobacterium abscessus in sample to be tested.
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| CN201810756530.7A CN108866216A (en) | 2018-07-11 | 2018-07-11 | It is a kind of for identifying the primer combination of probe of mycobacterium abscessus and Marseille mycobacteria |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109652572A (en) * | 2019-01-29 | 2019-04-19 | 首都医科大学附属北京胸科医院 | It is a kind of for detecting the primer combination of probe of mycobacterium abscessus and Marseille mycobacteria clarithromycin pharmaceutical sensibility |
| CN110938702A (en) * | 2019-11-28 | 2020-03-31 | 北京市结核病胸部肿瘤研究所 | Method for detecting mycobacterium avium by real-time fluorescent PCR and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102634575A (en) * | 2012-03-26 | 2012-08-15 | 中国人民解放军第三O九医院 | Rapid identification method and kit of novel mycobacterium strain |
| CN104561245A (en) * | 2013-10-16 | 2015-04-29 | 复旦大学 | Rapid identification method and kit for MTBC (mycobacterium tuberculosis complex) |
-
2018
- 2018-07-11 CN CN201810756530.7A patent/CN108866216A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102634575A (en) * | 2012-03-26 | 2012-08-15 | 中国人民解放军第三O九医院 | Rapid identification method and kit of novel mycobacterium strain |
| CN104561245A (en) * | 2013-10-16 | 2015-04-29 | 复旦大学 | Rapid identification method and kit for MTBC (mycobacterium tuberculosis complex) |
Non-Patent Citations (2)
| Title |
|---|
| TOIDI A等: "Reinstating mycobacterium massiliense and mycobacterium bolletii as species of the Mycobacterium acscessus complex", 《INT J SYST EVOL MICROBIOL》 * |
| 张智健等: "脓肿分枝杆菌复合群的研究进展", 《中国防痨杂志》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109652572A (en) * | 2019-01-29 | 2019-04-19 | 首都医科大学附属北京胸科医院 | It is a kind of for detecting the primer combination of probe of mycobacterium abscessus and Marseille mycobacteria clarithromycin pharmaceutical sensibility |
| CN110938702A (en) * | 2019-11-28 | 2020-03-31 | 北京市结核病胸部肿瘤研究所 | Method for detecting mycobacterium avium by real-time fluorescent PCR and application thereof |
| CN110938702B (en) * | 2019-11-28 | 2022-11-01 | 北京市结核病胸部肿瘤研究所 | Method for detecting mycobacterium avium by real-time fluorescent PCR and application thereof |
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