CN108865788A - One kind splitting formula leakproof out and quantifies surface sampling culture dish - Google Patents

Abstract

Formula leakproof out, which is split, the invention discloses one kind quantifies surface sampling culture dish, the culture dish includes ware bottom and ware lid, it can split set on the transversal crack mouth of the first ware downside wall and longitudinal crack mouth when stressed, it is detached from movable ware downside wall, the height of the first ware downside wall is caused to be lower than the plane of solid medium, therefore the operation of surface microorganism sampling is easier.In addition, the second ware downside wall can prevent culture medium from overflowing from ware bottom, what graduation mark can indicate agar medium pours into volume.

Description

One kind splitting formula leakproof out and quantifies surface sampling culture dish

Technical field

Formula leakproof out, which is split, the present invention relates to a kind of experiment equipment, especially one kind quantifies surface sampling culture dish.

Background technique

Culture dish is one of most widely used experiment equipment in biology, is usually used in the objects such as packaging material for food, tableware The culture of body surface microorganism is tested, and the sampling of body surface microorganism usually wipes determinand body surface with sterile cotton swab Cotton swab or filter paper are perhaps transferred in buffer with aseptic filter paper abutting object under test surface later and are eluted, to reach by face To the purpose of sampling.But cotton swab or filter paper can not discharge microorganism collected completely, be easy to cause Bacterial diversity Incomplete culture；In addition the sampling of body surface microorganism needs sterile working, and cotton swab or filter paper are carrying out microorganism Acquisition and elution during, be easy pollution by environmental microorganism, be extremely difficult to the requirement of sterile working；These disadvantages It will cause the deviation of body surface microorganism testing result.In addition, the operating process of the above method is cumbersome, inefficiency, hold Easily cause the waste of human resources.When the culture dish pours into agar medium, the volume of pouring into of culture medium is usually operator Depending on empirically, therefore the agar medium of different personnel pours into volume and may have larger difference, to influence relevant experiment As a result.

Summary of the invention

For disadvantages mentioned above, the purpose of the present invention is to provide the formula leakproofs out of splitting that a kind of structure is simple and convenient to operate to determine Scale surface sample culture dish.The implementation of the culture dish can simplify the sampling process of body surface microorganism, improve test result Accuracy, improve working efficiency.

Surface sampling culture dish is quantified for splitting formula leakproof out, to reach the purpose, the technical solution adopted by the present invention is that： One kind splitting formula leakproof out and quantifies surface sampling culture dish, the ware lid being covered on ware bottom including ware bottom and relaxation, it is characterised in that： The ware lid include tegmental wall and at least one from tegmental wall around the ware lid side wall holded up, the ware bottom includes bottom wall and at least One from bottom wall around the first ware downside wall holded up；The first ware downside wall is equipped at least one circle transversal crack mouth, institute The transversal crack choma stated around described first ware downside wall one week, the circle plane of the transversal crack mouth and the bottom wall it is flat Face is parallel；The first ware downside wall is divided into movable ware downside wall and fixed ware downside wall by the transversal crack mouth； The coupling part of the movable ware downside wall and the fixation ware downside wall is transversal crack wall, the transversal crack wall Thickness is less than the first ware downside wall；The movable ware downside wall be equipped at least one perpendicular to the transversal crack mouth longitudinal direction The movable ware downside wall of crackle mouth, longitudinal crack mouth two sides is connected by longitudinal crack wall, the thickness of the longitudinal crack wall Degree is less than the first ware downside wall；The diameter of the ware lid side wall is greater than the first ware downside wall；The diameter of the bottom wall is greater than The tegmental wall；The height of the activity ware downside wall and the sum of the height of the fixed ware downside wall, are less than the ware lid side wall Height；The culture dish further includes that at least one surround the second ware downside wall holded up from bottom wall, the second ware downside wall Height is less than the ware lid side wall, and the diameter of the second ware downside wall is greater than ware lid side wall, the first ware downside wall and the second ware bottom Side wall is located at the same side of the bottom wall；The first ware downside wall is additionally provided at least one graduation mark, the graduation mark Around the first ware downside wall one week, the circular flat of the graduation mark was parallel with the plane of the bottom wall.

Compared with the prior art, the beneficial effects of the invention are as follows：The longitudinal crack wall and transversal crack wall of the culture dish Thickness very little, be easy to rupture under external force, when applying force to the movable ware downside wall, the first ware bottom can be made Side wall splits at longitudinal crack mouth and transversal crack mouth, with removal activity ware downside wall, and then makes the height of the first ware downside wall Degree decreases below the plane of solid medium, and the solid culture base plane is directly covered in determinand body surface when use Face can reach the purpose of sampling microorganisms from surface of object.Therefore the structure of the culture dish is simple, easy to use, Ke Yijian The process for changing sampling microorganisms from surface of object overcomes the problems, such as to be easy to be polluted by environmental microorganism in sampling process, improve simultaneously Test result accuracy and working efficiency.In addition, the second ware downside wall can prevent culture medium from overflowing from ware bottom, graduation mark can refer to That shows agar medium pours into volume.

Detailed description of the invention

Illustrate technical solution of the present invention in order to clearer, it below will be to attached drawing needed in embodiment description Make brief introduction, it should be apparent that, drawings in the following description are only some embodiments of the invention, general for this field For logical technical staff, without creative efforts, it can also be obtained according to these attached drawings other attached drawings.

Fig. 1 is the structural schematic diagram for splitting formula leakproof out and quantifying surface sampling culture dish；Fig. 2 is the movable ware downside wall Cross-sectional view；Fig. 3 is the partial enlargement diagram of the first ware downside wall；Respectively number is respectively in figure：

1 is ware lid, and 2 be ware bottom, and 3 be tegmental wall, and 4 be bottom wall, and 5 be ware lid side wall, and 6 be the second ware downside wall, and 7 be the first ware bottom side Wall, 71 be movable ware downside wall, and 72 be transversal crack mouth, and 73 be transversal crack wall, and 74 be longitudinal crack mouth, and 75 be longitudinal crack Wall, 76 be fixed ware downside wall, and 13 be gap, and 22 be graduation mark.

Specific embodiment

It is right below in conjunction with the drawings and specific embodiments in order to be more clear the objectives, technical solutions, and advantages of the present invention The present invention is described in further detail.It should be appreciated that described embodiment is only used to explain the present invention, it is all to be based on Simplified or equivalent variations, all belongs to the scope of protection of the present invention made by technical solution of the present invention.

In the discussion of specific embodiment, it is described split formula leakproof out and quantify the shape of surface sampling culture dish preferably select Circle, still, those skilled in the art should be understood that it is suitable for such as ellipse, rectangle or other suitable Shape for culture dish.

Formula leakproof out is split the invention discloses one kind and quantifies surface sampling culture dish, including ware bottom 2 and relaxation are covered in ware Ware lid 1 on bottom 2, the ware lid 1 includes tegmental wall 3 and at least one surround the ware lid side wall 5 holded up from tegmental wall 3, described Ware bottom 2 includes bottom wall 4 and at least one surround the first ware downside wall 7 holded up from bottom wall 4；The first ware downside wall 7 is set There is at least one circle transversal crack mouth 72, the transversal crack mouth 72 around described first ware downside wall 7 one weeks, split by the transverse direction The circle plane of line mouth 72 is parallel with the plane of the bottom wall 4；The first ware downside wall 7 is by the transversal crack mouth 72 It is divided into movable ware downside wall 71 and fixed ware downside wall 76；The movable ware downside wall 71 and the fixation ware downside wall 76 coupling part is transversal crack wall 73, and the thickness of the transversal crack wall 73 is less than the first ware downside wall 7；The activity Ware downside wall 71 be equipped at least one perpendicular to the transversal crack mouth 72 longitudinal crack mouth 74,74 liang of the longitudinal crack mouth The movable ware downside wall 71 of side is connected by longitudinal crack wall 75, and the thickness of the longitudinal crack wall 75 is less than the first ware downside wall 7.Features described above splits the basic structure that formula leakproof out quantifies surface sampling culture dish described in having determined, wherein 75 He of longitudinal crack wall The thickness very little of transversal crack wall 73 is easy to rupture under external force, makes the first ware downside wall 7 in longitudinal crack mouth 74 and cross It splits to crackle mouth 72, movable ware downside wall 71 can be detached from from fixed ware downside wall 76 at this time.And the first ware downside wall 7 The space formed with bottom wall 4 is as culture inner cavity, when cultivating inner cavity by the enough agar mediums of injection（The culture medium Liquid level is higher than the transversal crack mouth 72）, after the culture medium solidification, apply force to the movable ware downside wall 71, so that the first ware downside wall 7 is split at longitudinal crack mouth 74 and transversal crack mouth 72, with removal activity ware downside wall 71, into And the height of fixed ware downside wall 76 is made to decrease below the plane of the agar medium, by the agar culture when use Base plane is directly covered in object under test surface, that is, can reach the purpose of sampling microorganisms from surface of object, avoid using cotton swab Son or filter paper carry out the tedious steps eluted again after microorganism acquisition, greatly simplify the mistake of sampling microorganisms from surface of object Journey, and overcome and be easy the problem of being polluted by environmental microorganism in sampling process, at the same improve test result accuracy and Working efficiency.

It is split in the embodiment that formula leakproof out quantifies surface sampling culture dish described, the diameter of the bottom wall 4 is greater than described The diameter of tegmental wall 3, the setting of this feature can be limited when ware lid 1 and the fastening of ware bottom 2, and ware lid side wall 5 can only be supported in bottom wall 4.In addition, the height of the activity ware downside wall 71 and the sum of the height of the fixed ware downside wall 76, are less than the ware lid side The height of wall 5, this feature can be limited to be existed centainly between activity ware downside wall 71 and tegmental wall 3 when ware lid 1 and the fastening of ware bottom 2 Gap, prevent the agar medium from contacting with tegmental wall 3.

The culture dish further includes that at least one surround the second ware downside wall 6 holded up, the first ware downside wall from bottom wall 4 7 and second ware downside wall 6 be located at the same side of the bottom wall 4.The diameter of the ware lid side wall 5 is greater than the first ware downside wall 7 Diameter, but less than the diameter of the second ware downside wall 6, this feature is limited when the ware lid 1 and the ware bottom 2 fastening, ware lid Side wall 5 is fastened on the centre of the first ware downside wall 7 and the second ware downside wall 6 just.In addition, the height of the second ware downside wall 6 is small In the ware lid side wall 5, this feature can prevent the second ware downside wall 6 from hindering the unlatching of the ware lid 1 with ware bottom 2 Or it fastens.First ware downside wall 7 and the second ware downside wall 6 and bottom wall 4 form gap 13, the gap 13 can collect to The agar medium of spill and leakage, prevents the culture medium from overflowing from ware bottom 2 when injecting agar medium in culture dish.

The first ware downside wall 7 is additionally provided at least one graduation mark 22, and the graduation mark 22 is around the first ware bottom Side wall 7 one weeks, the circular flat of the graduation mark 22 was parallel with the plane of the bottom wall 4；The graduation mark 22 can indicate Agar medium pours into volume, to avoid pouring into the difference of volume because of personal experience's difference bring.

Above by description of listed embodiment, the basic ideas and basic principles of the present invention are elaborated.Obviously, institute The embodiment of description is only a part of the embodiments of the present invention, and non-limiting exhaustion.Based on the embodiments of the present invention, originally Field those of ordinary skill all other embodiment obtained under the premise of not making the creative labor, belongs to this hair Bright protection scope.

Claims (1)

1. one kind, which splits formula leakproof out, quantifies surface sampling culture dish, special including the ware lid that ware bottom and relaxation are covered on ware bottom Sign is：The ware lid includes tegmental wall and at least one surround the ware lid side wall holded up from tegmental wall, and the ware bottom includes bottom Wall is at least one from bottom wall around the first ware downside wall holded up；The first ware downside wall is equipped at least one circle and laterally splits Line mouth, the transversal crack choma around described first ware downside wall one week, the circle plane of the transversal crack mouth with it is described The plane of bottom wall is parallel；The first ware downside wall is divided into movable ware downside wall and fixed ware by the transversal crack mouth Downside wall；The coupling part of the movable ware downside wall and the fixation ware downside wall is transversal crack wall, the transverse direction The thickness of crackle wall is less than the first ware downside wall；The movable ware downside wall is equipped at least one perpendicular to the transversal crack The longitudinal crack mouth of mouth, the movable ware downside wall of longitudinal crack mouth two sides are connected by longitudinal crack wall, and the longitudinal direction is split The thickness of line wall is less than the first ware downside wall；The diameter of the ware lid side wall is greater than the first ware downside wall；The bottom wall Diameter is greater than the tegmental wall；The height of the activity ware downside wall and the sum of the height of the fixed ware downside wall, are less than described The height of ware lid side wall；The culture dish further includes that at least one surround the second ware downside wall holded up, the second ware from bottom wall The height of downside wall is less than the ware lid side wall, and the diameter of the second ware downside wall is greater than ware lid side wall, the first ware downside wall and Second ware downside wall is located at the same side of the bottom wall；The first ware downside wall is additionally provided at least one graduation mark, described Graduation mark around the first ware downside wall one week, the circular flat of the graduation mark is parallel with the plane of the bottom wall.