CN108863882A - A method of astaxanthin is extracted using haematococcus pluvialis - Google Patents
A method of astaxanthin is extracted using haematococcus pluvialis Download PDFInfo
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- CN108863882A CN108863882A CN201811108620.1A CN201811108620A CN108863882A CN 108863882 A CN108863882 A CN 108863882A CN 201811108620 A CN201811108620 A CN 201811108620A CN 108863882 A CN108863882 A CN 108863882A
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- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C403/00—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone
- C07C403/24—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone having side-chains substituted by six-membered non-aromatic rings, e.g. beta-carotene
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
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Abstract
The invention discloses a kind of methods for extracting astaxanthin using haematococcus pluvialis, and steps are as follows：Haematococcus pluvialis powder is taken, sulfonic acid is added, is sent into autoclave handles together, then it puts into grinder, lye is added after milled processed, adjusts pH, is then sonicated, filtration, filter cake is taken, filter cake is washed, chloroform, ethyl alcohol and potassium hydroxide are added into filter cake C, homogenization, it stands, takes lower layer's chloroform phase, silica gel column chromatography purifying is sent into after vacuum concentration, then deionized water is added, after being uniformly mixed, stand, centrifugal treating, the solid matter that centrifugation is obtained is with being dried with nitrogen.The method that the present invention extracts astaxanthin using haematococcus pluvialis, can greatly improve the yield of astaxanthin, and the content of astaxanthin is high in product obtained, may advantageously facilitate the popularization and application of astaxanthin, have important market value and social value.
Description
Technical field
The present invention relates to Astaxanthin extraction technical field, specifically a kind of side that astaxanthin is extracted using haematococcus pluvialis
Method.
Background technique
Astaxanthin also known as astaxanthin, Astaxanthin are the highests of a Carotenoids and carotenogenesis
Rank product, is in rediance, and chemical structure is similar to beta carotene.And beta carotene, lutein, canthaxanthin, tomato red
Element etc. is all the intermediate product of carotenogenesis, therefore in nature, astaxanthin has strongest inoxidizability.It deposits extensively
It is in the feather of living nature, especially shrimp, crab, fish, frond, yeast and birds that content is higher, is main in marine organisms body
One of carotenoid.
Astaxanthin can have anti-oxidant, anti-aging, antitumor, prevention heart and brain in conjunction with protein and in green, blue in vivo
The effect of vascular diseases.Microalgae of the astaxanthin from referred to as haematococcus clothing, this kind of seaweed pole in polar ocean environment
Horn of plenty, and be a kind of natural carotenoid (from yellow to red any one, including carrotene and xanthopicrin).
Astaxanthin is one of xanthopicrin race of carotenoid section.Xanthopicrin helps to prevent VitAVitE and others
Carotenoid aoxidizes.Xanthopicrin is that antioxidant effect is strongest in all carotenoid, 10 times stronger than beta carotene,
It is 100 times stronger than vitamin E.
It is confirmed through nutritionist, astaxanthin can be across the barrier from blood to brain, so astaxanthin is to brain, maincenter
Nervous system and eyes can all play a protective role.There are also other effects for astaxanthin：Physical endurance is improved, muscle damage is reduced
Risk；Alleviate asthenopia symptom, improves visual sensitivity；It is supplemented by inside and reduces wrinkle；Alleviate the disease of pigment over-deposit
Shape (i.e. usually said senile plaque)；Cell factor is adjusted, the expression of inflammatory cytokine and chemokines is inhibited；Improve
Stomach health alleviates infection or inflammation that helicobacter pylori causes.
The production of astaxanthin has artificial synthesized and biological acquisition two ways.Not only price is high for artificial synthesized astaxanthin
It is expensive, and with natural astaxanthin structure, function, application and in terms of significant difference.Therefore it is shrimp blueness that biology, which obtains,
The research hotspot of plain preparation field.
The biological source of natural astaxanthin generally has 3 kinds：Waste, phaffiafhodozyma and the Yu Sheng of processing of aquatic products industry
Haematococcus.Wherein, content astaxanthin is lower in waste, and extraction is costly, is unsuitable for being mass produced.Natural
Astaxanthin in phaffiarhodozyma average content is also only 0.40%.In contrast, Determination of Astaxanthin in Haematococcus Pluvialis content is but up to
1.5%~3.0%, therefore it is counted as " concentrate " of natural astaxanthin.Astaxanthin is extracted in research from haematococcus pluvialis
Method has important market value and social value.The existing method that astaxanthin is extracted from haematococcus pluvialis is generally existing
The low defect of yield, influences the popularization and application of astaxanthin.
Summary of the invention
The purpose of the present invention is to provide a kind of methods for extracting astaxanthin using haematococcus pluvialis, to solve above-mentioned background
The problem of being proposed in technology.
To achieve the above object, the present invention provides the following technical solutions：
A method of astaxanthin being extracted using haematococcus pluvialis, steps are as follows：
1) haematococcus pluvialis powder is taken, sulfonic acid is added, is then sent into autoclave together, in 40-50Mpa, 6-10 DEG C
Lower processing 20-30min, discharging obtain mixture A；
2) mixture A is put into grinder, milled processed 50-60min, is discharged, obtain mixture B；
3) to lye will be added in mixture B, adjusting pH is 7.0-7.5, is then fed into processor for ultrasonic wave, at ultrasound
40-45min is managed, filtration takes filter cake, and filter cake is washed 2-3 times, obtains mixture C；
4) chloroform, ethyl alcohol and potassium hydroxide are added into mixture C, is then fed into high pressure homogenizer, at homogeneous
32-35min is managed, mixture D is obtained；
5) mixture D is stood at 4 DEG C 10-15h, takes lower layer's chloroform phase, and be concentrated in vacuo at 45-50 DEG C,
Obtain mixture E；
6) by mixture E be sent into silica gel column chromatography purifying, elution when, first rinsed with n-hexane it is colourless to efflux, so
It is eluted afterwards with n-hexane-acetone mixture, obtains mixture F；
7) deionized water is added into mixture F, after being uniformly mixed, 24-30h is stood at -4 DEG C, is then carried out
Centrifugal treating, the solid matter that centrifugation is obtained is with being dried with nitrogen.
As a further solution of the present invention：In step 1), the sulfonic acid is the methanesulfonic acid of concentration 32%.
As further scheme of the invention：In step 1), the dosage of the sulfonic acid is haematococcus pluvialis powder total weight
1.5-2.0 times.
As further scheme of the invention：In step 3), the lye is the sodium hydroxide solution of concentration 20%.
As further scheme of the invention：In step 4), the dosage of the chloroform is mixture C total volume
80-90%.
As further scheme of the invention：In step 4), the dosage of the ethyl alcohol is the 20- of mixture C total volume
25%.
As further scheme of the invention：In step 4), the dosage of the potassium hydroxide is that haematococcus pluvialis powder is total
The 31-35% of weight.
As further scheme of the invention：In step 4), the pressure of the high pressure homogenizer is first to use 15-
The pressure treatment 20min of 16Mpa, then use the pressure treatment 12-15min of 5.0-5.5Mpa.
As further scheme of the invention：In step 6), when being eluted using n-hexane-acetone mixture,
Successively use volume ratio for 5:1,3:1,1:1 n-hexane-acetone mixture is eluted.
As further scheme of the invention：In step 7), the revolving speed of the centrifugal treating is 4500-4800rpm.
Compared with prior art, the beneficial effects of the invention are as follows：
The method that the present invention extracts astaxanthin using haematococcus pluvialis, can greatly improve the yield of astaxanthin, be obtained
Product in astaxanthin content it is high, may advantageously facilitate the popularization and application of astaxanthin, with important market value and social valence
Value.
Specific embodiment
Technical solution of the present invention is described in more detail With reference to embodiment.
Embodiment 1
A method of astaxanthin being extracted using haematococcus pluvialis, steps are as follows：
1) haematococcus pluvialis powder is taken, sulfonic acid is added, is then sent into autoclave, is handled at 40Mpa, 6 DEG C together
20min, discharging obtain mixture A, wherein the sulfonic acid is the methanesulfonic acid of concentration 32%, and the dosage of the sulfonic acid is that rain life is red
1.5 times of ball algae powder total weight；
2) mixture A is put into grinder, milled processed 50min, is discharged, obtain mixture B；
3) to lye will be added in mixture B, adjusting pH is 7.0, is then fed into processor for ultrasonic wave, is ultrasonically treated
40min, filtration, takes filter cake, and filter cake is washed 2 times, obtains mixture C, wherein the lye is the sodium hydroxide of concentration 20%
Solution；
4) chloroform, ethyl alcohol and potassium hydroxide are added into mixture C, is then fed into high pressure homogenizer, at homogeneous
32min is managed, mixture D is obtained, wherein the dosage of the chloroform is the 80% of mixture C total volume, the use of the ethyl alcohol
Amount is the 20% of mixture C total volume, and the dosage of the potassium hydroxide is the 31% of haematococcus pluvialis powder total weight, the high pressure
The pressure of homogenizer is the pressure treatment 20min for first using 15Mpa, then uses the pressure treatment 12min of 5.0Mpa；
5) mixture D is stood at 4 DEG C 10h, takes lower layer's chloroform phase, and be concentrated in vacuo at 45 DEG C, is mixed
Close object E；
6) by mixture E be sent into silica gel column chromatography purifying, elution when, first rinsed with n-hexane it is colourless to efflux, so
It is eluted afterwards with n-hexane-acetone mixture, obtains mixture F, wherein washed using n-hexane-acetone mixture
When de-, successively use volume ratio for 5:1,3:1,1:1 n-hexane-acetone mixture is eluted；
7) deionized water is added into mixture F, after being uniformly mixed, stands at -4 DEG C for 24 hours, be then centrifuged
Processing, the solid matter that centrifugation is obtained is with being dried with nitrogen, wherein the revolving speed of the centrifugal treating is 4500rpm.
Embodiment 2
A method of astaxanthin being extracted using haematococcus pluvialis, steps are as follows：
1) haematococcus pluvialis powder is taken, sulfonic acid is added, is then sent into autoclave, is handled at 42Mpa, 6 DEG C together
23min, discharging obtain mixture A, wherein the sulfonic acid is the methanesulfonic acid of concentration 32%, and the dosage of the sulfonic acid is that rain life is red
1.6 times of ball algae powder total weight；
2) mixture A is put into grinder, milled processed 52min, is discharged, obtain mixture B；
3) to lye will be added in mixture B, adjusting pH is 7.1, is then fed into processor for ultrasonic wave, is ultrasonically treated
43min, filtration, takes filter cake, and filter cake is washed 2 times, obtains mixture C, wherein the lye is the sodium hydroxide of concentration 20%
Solution；
4) chloroform, ethyl alcohol and potassium hydroxide are added into mixture C, is then fed into high pressure homogenizer, at homogeneous
32min is managed, mixture D is obtained, wherein the dosage of the chloroform is the 85% of mixture C total volume, the use of the ethyl alcohol
Amount is the 25% of mixture C total volume, and the dosage of the potassium hydroxide is the 32% of haematococcus pluvialis powder total weight, the high pressure
The pressure of homogenizer is the pressure treatment 20min for first using 15Mpa, then uses the pressure treatment 13min of 5.3Mpa；
5) mixture D is stood at 4 DEG C 11h, takes lower layer's chloroform phase, and be concentrated in vacuo at 45 DEG C, is mixed
Close object E；
6) by mixture E be sent into silica gel column chromatography purifying, elution when, first rinsed with n-hexane it is colourless to efflux, so
It is eluted afterwards with n-hexane-acetone mixture, obtains mixture F, wherein washed using n-hexane-acetone mixture
When de-, successively use volume ratio for 5:1,3:1,1:1 n-hexane-acetone mixture is eluted；
7) deionized water is added into mixture F, after being uniformly mixed, 25h is stood at -4 DEG C, is then centrifuged
Processing, the solid matter that centrifugation is obtained is with being dried with nitrogen, wherein the revolving speed of the centrifugal treating is 4600rpm.
Embodiment 3
A method of astaxanthin being extracted using haematococcus pluvialis, steps are as follows：
1) haematococcus pluvialis powder is taken, sulfonic acid is added, is then sent into autoclave, is handled at 45Mpa, 8 DEG C together
25min, discharging obtain mixture A, wherein the sulfonic acid is the methanesulfonic acid of concentration 32%, and the dosage of the sulfonic acid is that rain life is red
1.8 times of ball algae powder total weight；
2) mixture A is put into grinder, milled processed 55min, is discharged, obtain mixture B；
3) to lye will be added in mixture B, adjusting pH is 7.3, is then fed into processor for ultrasonic wave, is ultrasonically treated
42min, filtration, takes filter cake, and filter cake is washed 3 times, obtains mixture C, wherein the lye is the sodium hydroxide of concentration 20%
Solution；
4) chloroform, ethyl alcohol and potassium hydroxide are added into mixture C, is then fed into high pressure homogenizer, at homogeneous
34min is managed, mixture D is obtained, wherein the dosage of the chloroform is the 85% of mixture C total volume, the use of the ethyl alcohol
Amount is the 23% of mixture C total volume, and the dosage of the potassium hydroxide is the 33% of haematococcus pluvialis powder total weight, the high pressure
The pressure of homogenizer is the pressure treatment 20min for first using 15.5Mpa, then uses the pressure treatment 14min of 5.3Mpa；
5) mixture D is stood at 4 DEG C 12h, takes lower layer's chloroform phase, and be concentrated in vacuo at 48 DEG C, is mixed
Close object E；
6) by mixture E be sent into silica gel column chromatography purifying, elution when, first rinsed with n-hexane it is colourless to efflux, so
It is eluted afterwards with n-hexane-acetone mixture, obtains mixture F, wherein washed using n-hexane-acetone mixture
When de-, successively use volume ratio for 5:1,3:1,1:1 n-hexane-acetone mixture is eluted；
7) deionized water is added into mixture F, after being uniformly mixed, 28h is stood at -4 DEG C, is then centrifuged
Processing, the solid matter that centrifugation is obtained is with being dried with nitrogen, wherein the revolving speed of the centrifugal treating is 4700rpm.
Embodiment 4
A method of astaxanthin being extracted using haematococcus pluvialis, steps are as follows：
1) haematococcus pluvialis powder is taken, sulfonic acid is added, is then sent into autoclave, is handled at 47Mpa, 10 DEG C together
25min, discharging obtain mixture A, wherein the sulfonic acid is the methanesulfonic acid of concentration 32%, and the dosage of the sulfonic acid is that rain life is red
1.9 times of ball algae powder total weight；
2) mixture A is put into grinder, milled processed 57min, is discharged, obtain mixture B；
3) to lye will be added in mixture B, adjusting pH is 7.5, is then fed into processor for ultrasonic wave, is ultrasonically treated
45min, filtration, takes filter cake, and filter cake is washed 2 times, obtains mixture C, wherein the lye is the sodium hydroxide of concentration 20%
Solution；
4) chloroform, ethyl alcohol and potassium hydroxide are added into mixture C, is then fed into high pressure homogenizer, at homogeneous
35min is managed, mixture D is obtained, wherein the dosage of the chloroform is the 85% of mixture C total volume, the use of the ethyl alcohol
Amount is the 20% of mixture C total volume, and the dosage of the potassium hydroxide is the 34% of haematococcus pluvialis powder total weight, the high pressure
The pressure of homogenizer is the pressure treatment 20min for first using 16Mpa, then uses the pressure treatment 14min of 5.5Mpa；
5) mixture D is stood at 4 DEG C 13h, takes lower layer's chloroform phase, and be concentrated in vacuo at 48 DEG C, is mixed
Close object E；
6) by mixture E be sent into silica gel column chromatography purifying, elution when, first rinsed with n-hexane it is colourless to efflux, so
It is eluted afterwards with n-hexane-acetone mixture, obtains mixture F, wherein washed using n-hexane-acetone mixture
When de-, successively use volume ratio for 5:1,3:1,1:1 n-hexane-acetone mixture is eluted；
7) deionized water is added into mixture F, after being uniformly mixed, 28h is stood at -4 DEG C, is then centrifuged
Processing, the solid matter that centrifugation is obtained is with being dried with nitrogen, wherein the revolving speed of the centrifugal treating is 4800rpm.
Embodiment 5
A method of astaxanthin being extracted using haematococcus pluvialis, steps are as follows：
1) haematococcus pluvialis powder is taken, sulfonic acid is added, is then sent into autoclave, is handled at 50Mpa, 10 DEG C together
30min, discharging obtain mixture A, wherein the sulfonic acid is the methanesulfonic acid of concentration 32%, and the dosage of the sulfonic acid is that rain life is red
2.0 times of ball algae powder total weight；
2) mixture A is put into grinder, milled processed 60min, is discharged, obtain mixture B；
3) to lye will be added in mixture B, adjusting pH is 7.5, is then fed into processor for ultrasonic wave, is ultrasonically treated
45min, filtration, takes filter cake, and filter cake is washed 3 times, obtains mixture C, wherein the lye is the sodium hydroxide of concentration 20%
Solution；
4) chloroform, ethyl alcohol and potassium hydroxide are added into mixture C, is then fed into high pressure homogenizer, at homogeneous
35min is managed, mixture D is obtained, wherein the dosage of the chloroform is the 90% of mixture C total volume, the use of the ethyl alcohol
Amount is the 25% of mixture C total volume, and the dosage of the potassium hydroxide is the 35% of haematococcus pluvialis powder total weight, the high pressure
The pressure of homogenizer is the pressure treatment 20min for first using 16Mpa, then uses the pressure treatment 15min of 5.5Mpa；
5) mixture D is stood at 4 DEG C 15h, takes lower layer's chloroform phase, and be concentrated in vacuo at 50 DEG C, is mixed
Close object E；
6) by mixture E be sent into silica gel column chromatography purifying, elution when, first rinsed with n-hexane it is colourless to efflux, so
It is eluted afterwards with n-hexane-acetone mixture, obtains mixture F, wherein washed using n-hexane-acetone mixture
When de-, successively use volume ratio for 5:1,3:1,1:1 n-hexane-acetone mixture is eluted；
7) deionized water is added into mixture F, after being uniformly mixed, 30h is stood at -4 DEG C, is then centrifuged
Processing, the solid matter that centrifugation is obtained is with being dried with nitrogen, wherein the revolving speed of the centrifugal treating is 4800rpm.
Haematococcus pluvialis is extracted using 1-5 extracting method of the embodiment of the present invention, wherein the shrimp of haematococcus pluvialis powder
Green cellulose content is 1.85%.After extracting, in product obtained, the content of astaxanthin is 10.2-11.5%, astaxanthin
Recovery rate is 94.6-97.3%.Wherein, the content astaxanthin in the content astaxanthin and product in haematococcus pluvialis powder is all made of
Light absorption value method is measured (referring to West Asia promise Imtech).
The method that the present invention extracts astaxanthin using haematococcus pluvialis, can greatly improve the yield of astaxanthin, be obtained
Product in astaxanthin content it is high, may advantageously facilitate the popularization and application of astaxanthin, with important market value and social valence
Value.
Better embodiment of the invention is explained in detail above, but the present invention is not limited to above-mentioned embodiment party
Formula within the knowledge of one of ordinary skill in the art can also be without departing from the purpose of the present invention
Various changes can be made.
Claims (10)
1. a kind of method for extracting astaxanthin using haematococcus pluvialis, which is characterized in that steps are as follows：
1) haematococcus pluvialis powder is taken, sulfonic acid is added, is then sent into autoclave together, at 40-50Mpa, 6-10 DEG C
20-30min is managed, discharging obtains mixture A；
2) mixture A is put into grinder, milled processed 50-60min, is discharged, obtain mixture B；
3) to lye will be added in mixture B, adjusting pH is 7.0-7.5, is then fed into processor for ultrasonic wave, is ultrasonically treated
40-45min, filtration, takes filter cake, and filter cake is washed 2-3 times, obtains mixture C；
4) chloroform, ethyl alcohol and potassium hydroxide are added into mixture C, is then fed into high pressure homogenizer, homogenization 32-
35min obtains mixture D；
5) mixture D is stood at 4 DEG C 10-15h, takes lower layer's chloroform phase, and be concentrated in vacuo at 45-50 DEG C, obtained
Mixture E；
6) mixture E is sent into silica gel column chromatography purifying and is first rinsed with n-hexane colourless to efflux in elution, then used
N-hexane-acetone mixture is eluted, and mixture F is obtained；
7) deionized water is added into mixture F, after being uniformly mixed, 24-30h is stood at -4 DEG C, is then centrifuged
Processing, the solid matter that centrifugation is obtained is with being dried with nitrogen.
2. the method according to claim 1 for extracting astaxanthin using haematococcus pluvialis, which is characterized in that in step 1),
The sulfonic acid is the methanesulfonic acid of concentration 32%.
3. the method according to claim 2 for extracting astaxanthin using haematococcus pluvialis, which is characterized in that in step 1),
The dosage of the sulfonic acid is 1.5-2.0 times of haematococcus pluvialis powder total weight.
4. the method according to claim 1 for extracting astaxanthin using haematococcus pluvialis, which is characterized in that in step 3),
The lye is the sodium hydroxide solution of concentration 20%.
5. the method according to claim 1 to 4 for extracting astaxanthin using haematococcus pluvialis, which is characterized in that step
4) in, the dosage of the chloroform is the 80-90% of mixture C total volume.
6. the method according to claim 5 for extracting astaxanthin using haematococcus pluvialis, which is characterized in that in step 4),
The dosage of the ethyl alcohol is the 20-25% of mixture C total volume.
7. the method according to claim 6 for extracting astaxanthin using haematococcus pluvialis, which is characterized in that in step 4),
The dosage of the potassium hydroxide is the 31-35% of haematococcus pluvialis powder total weight.
8. the method according to claim 7 for extracting astaxanthin using haematococcus pluvialis, which is characterized in that in step 4),
The pressure of the high pressure homogenizer be first use 15-16Mpa pressure treatment 20min, then using 5.0-5.5Mpa pressure at
Manage 12-15min.
9. the method according to claim 1 for extracting astaxanthin using haematococcus pluvialis, which is characterized in that in step 6),
When being eluted using n-hexane-acetone mixture, successively use volume ratio for 5:1,3:1,1:1 n-hexane-acetone is mixed
Liquid is closed to be eluted.
10. the method according to claim 1 for extracting astaxanthin using haematococcus pluvialis, which is characterized in that in step 7),
The revolving speed of the centrifugal treating is 4500-4800rpm.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111440106A (en) * | 2020-05-06 | 2020-07-24 | 云南爱尔康生物技术有限公司 | Method for adsorbing and separating natural astaxanthin ester |
| CN111778170A (en) * | 2019-04-03 | 2020-10-16 | 中国农业大学 | Bacillus belgii and application thereof |
| CN111778227A (en) * | 2019-04-03 | 2020-10-16 | 中国农业大学 | Astaxanthin esterase and preparation method of astaxanthin monomer |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101904873A (en) * | 2010-08-05 | 2010-12-08 | 郭景龙 | Medicament taking haematococcus pluvialis as raw material |
| CN103232375A (en) * | 2013-04-03 | 2013-08-07 | 大连医诺生物有限公司 | Novel high-efficiency extraction process for astaxanthin in Haematococcus pluvialis |
| CN106467479A (en) * | 2016-08-30 | 2017-03-01 | 中国科学院水生生物研究所 | A kind of method extracting astaxanthin from Haematocoocus Pluvialls |
-
2018
- 2018-09-21 CN CN201811108620.1A patent/CN108863882A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101904873A (en) * | 2010-08-05 | 2010-12-08 | 郭景龙 | Medicament taking haematococcus pluvialis as raw material |
| CN103232375A (en) * | 2013-04-03 | 2013-08-07 | 大连医诺生物有限公司 | Novel high-efficiency extraction process for astaxanthin in Haematococcus pluvialis |
| CN106467479A (en) * | 2016-08-30 | 2017-03-01 | 中国科学院水生生物研究所 | A kind of method extracting astaxanthin from Haematocoocus Pluvialls |
Non-Patent Citations (2)
| Title |
|---|
| 蔡小连等: "从雨声红球藻中提取虾青素的皂化工艺优化", 《山东化工》 * |
| 赵晓燕等: "雨生红球藻中虾青素的研究进展", 《食品研究与开发》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111778170A (en) * | 2019-04-03 | 2020-10-16 | 中国农业大学 | Bacillus belgii and application thereof |
| CN111778227A (en) * | 2019-04-03 | 2020-10-16 | 中国农业大学 | Astaxanthin esterase and preparation method of astaxanthin monomer |
| CN111778170B (en) * | 2019-04-03 | 2022-03-29 | 中国农业大学 | Bacillus belgii and application thereof |
| CN111440106A (en) * | 2020-05-06 | 2020-07-24 | 云南爱尔康生物技术有限公司 | Method for adsorbing and separating natural astaxanthin ester |
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