CN108850549A - A kind of defatted rice bran Multi-strain fermentation technology - Google Patents

A kind of defatted rice bran Multi-strain fermentation technology Download PDF

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Publication number
CN108850549A
CN108850549A CN201810660404.1A CN201810660404A CN108850549A CN 108850549 A CN108850549 A CN 108850549A CN 201810660404 A CN201810660404 A CN 201810660404A CN 108850549 A CN108850549 A CN 108850549A
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CN
China
Prior art keywords
rice bran
defatted rice
strain
composite bacteria
fermentation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810660404.1A
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Chinese (zh)
Inventor
姚远
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tianzhu County Senyuan Rice Industry LLC
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Tianzhu County Senyuan Rice Industry LLC
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Publication date
Application filed by Tianzhu County Senyuan Rice Industry LLC filed Critical Tianzhu County Senyuan Rice Industry LLC
Priority to CN201810660404.1A priority Critical patent/CN108850549A/en
Publication of CN108850549A publication Critical patent/CN108850549A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/21Streptococcus, lactococcus
    • A23V2400/249Thermophilus
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Abstract

The present invention relates to a kind of defatted rice bran Multi-strain fermentation technologies, include the following steps:(1) defatted rice bran is crushed to 40-80 mesh, 100-120 degrees Celsius sterilizing 10-30 seconds;(2) pure water is added into treated defatted rice bran, water content is set to reach 20-40%, composite bacteria is accessed thereto, the mass percent that inoculum concentration accounts for defatted rice bran is 0.5%-1.5%, control temperature 30-40 degrees Celsius fermentation 12-24 hours, the composite bacteria is by trichodermaharzianum, geotrichum candidum, streptococcus thermophilus according to 2-3:1-2:The ratio of 1-2 forms;(3) product after fermentation is lower than 12% in 40-55 degrees Celsius of drying of temperature to water content, defatted rice bran tunning is made after crushing.The animals such as the nonprotein nitrogen (such as small peptide, free amino acid) of defatted rice bran can be improved by composite bacteria fermentation technique by the present invention can utilize the content of protein sources, reduce crude fiber level, and improve polyoses content.

Description

A kind of defatted rice bran Multi-strain fermentation technology
Technical field
The invention belongs to technical field of biological fermentation, more particularly, to a kind of defatted rice bran Multi-strain fermentation technology.
Background technique
For the annual paddy total output in China at 200,000,000 tons or so, by-product of the rice bran as Rice producing is annual to produce about 1800 Ten thousand tons of rice bran accounts for about the 10% of brown rice weight.Rice bran is mainly made of kind of a skin, megarchidium layer, aleurone and embryo.Due in rice bran Containing the environmental contaminants in various enzymes and mill processes, it quickly can souring corruption.Therefore, most of rice bran can be by honest and clean at once Valence is sold as feed or fertilizer.Although there are these disadvantages for rice bran, due to rice bran nutrition rich in (sugar 33.5-53.5%, protein 11.5-17.2%, grease 12.8-22.6% and minerals and vitamin etc.), also it is rich in phenol The active components of plants such as substance are a kind of " nutrient sources of being bestowed by heaven ".
Defatted rice bran is using the byproduct of rice bran production rice bran oil, and skimming processes make the lipase in rice bran lose work Property, the undesirable substances such as fungi, the bacterium in rice bran are eliminated, the natural nutrient component of rice bran is remained.Most domestic at present Defatted rice bran is only used as feedstuff use, and added value is lower, and protein component therein fails to be reprocessed and integrated benefit With.Rice bran protein is to generally acknowledge quality plant protein, and essential amino acid Compositional balance is reasonable, close to FAO/WHO recommendation pattern; Wherein lysine content is higher, is that other vegetable proteins are incomparable, and rice bran protein biological value is very high, and nutritive value can be with Egg, cow's milk compare favourably, and digestibility is up to 90% or more.Rice bran protein is mainly by albumin, globulin, alcohol soluble protein and paddy Four part of albumen composition, rice bran protein affect dissolubility in aqueous solution, belong to molten because itself containing more disulfide bond The lower protein of solution property.
It is increasingly taken seriously, can be comprehensively utilized using reasonable technique to mention to the comprehensive utilization of rice bran resource Its added value of height.By the research to defatted rice bran Multi-strain fermentation technology, can be applied in rice by-product deep processing, energy Enough significantly improve the nutritive value and biology availability of defatted rice bran.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of defatted rice bran Multi-strain fermentation technology, it can be used for rice pair In product deep processing, the nutritive value and biology availability of defatted rice bran can be significantly improved.
The present invention is achieved by the following technical programs.
The present invention provides a kind of defatted rice bran Multi-strain fermentation technology, which is characterized in that includes the following steps:
(1) defatted rice bran is crushed to 40-80 mesh, 100-120 degrees Celsius sterilizing 10-30 seconds;
(2) pure water is added into treated defatted rice bran, so that water content is reached 20-40%, accesses thereto compound Strain, the mass percent that inoculum concentration accounts for defatted rice bran is 0.5%-1.5%, controls temperature in 30-40 degrees Celsius of fermentation 12-24 Hour, the composite bacteria is by trichodermaharzianum, geotrichum candidum, streptococcus thermophilus according to 2-3:1-2:The ratio of 1-2 forms;
(3) product after fermentation is lower than 12% in 40-55 degrees Celsius of drying of temperature to water content, degreasing is made after crushing Rice bran tunning.
Further, the composite bacteria is by trichodermaharzianum, geotrichum candidum, streptococcus thermophilus according to 2:1:1 ratio group At.
Further, the number of viable of each strain is respectively in the composite bacteria:Trichodermaharzianum not less than 1 × 107Cfu/mL, geotrichum candidum are not less than 1 × 107Cfu/mL, streptococcus thermophilus are not less than 1 × 108cfu/mL。
Further, in the composite bacteria single culture used from each strain commercially available single active bacteria formulation, or Person passes through routine culture, the microbial inoculum of the numerous preparation of expansion using any known bacterial strain for being accredited as corresponding strain through microbiology.
Compared with prior art, the beneficial effect that the present invention obtains includes:
Nonprotein nitrogen (such as the small peptide, free ammonia of defatted rice bran can be improved by composite bacteria fermentation technique by the present invention Base acid) etc. animals can utilize the contents of protein sources, reduce crude fiber level, and improve polyoses content, to be inhaled for animal digestion It receives nutrient therein and provides good basis.Also, by comparison, it was found that the composite bacteria being made of three kinds of strains relative to The combination of single culture or two kinds of strains achieves more significant progress effect, shows that these three strains are de- for fermenting There is synergistic effect, it may be possible to since these three strains can promote mutually bacterial activity to more in fermentation for rouge rice bran It is related that the biodegradable enzymes such as protease, cellulase, pectase are generated at a high level.
Specific embodiment
Technical solution of the present invention is described further below by specific embodiment, but following specific embodiments do not have to In the limitation present invention.It is all within spirit of that invention and principle, any modification, equivalent replacement, improvement and so on should all include Within protection scope of the present invention.
Embodiment 1
A kind of defatted rice bran Multi-strain fermentation technology, which is characterized in that include the following steps:
(1) defatted rice bran is crushed to 60 mesh, 110 degrees Celsius sterilize 20 seconds;
(2) pure water is added into treated defatted rice bran, so that water content is reached 30%, accesses compound bacteria thereto Kind, the mass percent that inoculum concentration accounts for defatted rice bran is 1.0%, and control temperature is fermented 18 hours at 35 degrees Celsius, described compound Strain is by trichodermaharzianum, geotrichum candidum, streptococcus thermophilus according to 2:1:1 ratio composition;
(3) product after fermentation is lower than 12% in 50 degrees Celsius of dryings of temperature to water content, degreasing rice is made after crushing Chaff tunning.
The number of viable of each strain is respectively in the composite bacteria:Trichodermaharzianum is not less than 1 × 107Cfu/mL, Bai Di It is mould to be not less than 1 × 107Cfu/mL, streptococcus thermophilus are not less than 1 × 108cfu/mL;Single culture used is next in the composite bacteria From in the commercially available single active bacteria formulation of each strain.
Embodiment 2
A kind of defatted rice bran Multi-strain fermentation technology, other than composite bacteria used only includes trichodermaharzianum, other steps Suddenly embodiment 1 is same as with parameter.
Embodiment 3
A kind of defatted rice bran Multi-strain fermentation technology, other than composite bacteria used only includes geotrichum candidum, other steps Embodiment 1 is same as with parameter.
Embodiment 4
A kind of defatted rice bran Multi-strain fermentation technology, other than composite bacteria used only includes streptococcus thermophilus, other Step and parameter are same as embodiment 1.
Embodiment 5
A kind of defatted rice bran Multi-strain fermentation technology, in addition to composite bacteria used is by trichodermaharzianum, geotrichum candidum according to 2:1 Ratio composition except, other steps and parameter are same as embodiment 1.
Embodiment 6
A kind of defatted rice bran Multi-strain fermentation technology, in addition to composite bacteria used be by trichodermaharzianum, streptococcus thermophilus by According to 2:Except 1 ratio composition, other steps and parameter are same as embodiment 1.
Embodiment 7
A kind of defatted rice bran Multi-strain fermentation technology, in addition to composite bacteria used be by geotrichum candidum, streptococcus thermophilus according to 1:Except 1 ratio composition, other steps and parameter are same as embodiment 1.
Embodiment 8
Using defatted rice bran made from embodiment 1-7 as 7 test groups, as a control group by raw material defatted rice bran, measurement Nutrient content in this 8 groups of samples, measuring method is using nutrient measuring method well known to field of fodder.Measurement result is referring to following table 1。
As shown in Table 1, the nonprotein nitrogen (such as small peptide, free amino acid) of defatted rice bran can be improved by strain fermentation Equal animals can utilize the content of protein sources, reduce crude fiber level, and improve polyoses content, to absorb wherein for animal digestion Nutrient provide good basis.Also, by comparison, it was found that the composite bacteria being made of three kinds of strains is relative to single bacterium The combination of kind or two kinds of strains achieves more significant progress effect, shows these three strains for the defatted rice bran that ferments For there is synergistic effect, it may be possible to since these three strains can promote mutually bacterial activity to higher level in fermentation The biodegradable enzymes such as real estate raw albumen enzyme, cellulase, pectase are related.
Concrete scheme and main feature of the invention has been shown and described above, and to a certain extent by test data Embody advantages of the present invention.It should be understood by those skilled in the art that the present invention is not limited to the above embodiments, above-mentioned reality It applies and only illustrates concrete scheme of the invention described in example and specification, in the premise for not departing from spirit and scope of the invention Under, various changes and improvements may be made to the invention, and these changes and improvements all fall within the protetion scope of the claimed invention.This hair Bright claimed range is defined by the appending claims and its equivalent thereof.

Claims (4)

1. a kind of defatted rice bran Multi-strain fermentation technology, which is characterized in that include the following steps:
(1) defatted rice bran is crushed to 40-80 mesh, 100-120 degrees Celsius sterilizing 10-30 seconds;
(2) pure water is added into treated defatted rice bran, so that water content is reached 20-40%, accesses composite bacteria thereto, Inoculum concentration account for defatted rice bran mass percent be 0.5%-1.5%, control temperature 30-40 degrees Celsius fermentation 12-24 hours, The composite bacteria is by trichodermaharzianum, geotrichum candidum, streptococcus thermophilus according to 2-3:1-2:The ratio of 1-2 forms;
(3) product after fermentation is lower than 12% in 40-55 degrees Celsius of drying of temperature to water content, defatted rice bran is made after crushing Tunning.
2. defatted rice bran Multi-strain fermentation technology as described in claim 1, which is characterized in that the composite bacteria is by healthy and free from worry Trichoderma, geotrichum candidum, streptococcus thermophilus are according to 2:1:1 ratio composition.
3. defatted rice bran Multi-strain fermentation technology as described in claim 1, which is characterized in that each strain in the composite bacteria Number of viable be respectively:Trichodermaharzianum is not less than 1 × 107Cfu/mL, geotrichum candidum are not less than 1 × 107Cfu/mL, thermophilus Bacterium is not less than 1 × 108cfu/mL。
4. defatted rice bran Multi-strain fermentation technology as described in claim 1, which is characterized in that list used in the composite bacteria One strain from each strain commercially available single active bacteria formulation, or using through microbiology be accredited as corresponding strain it is any The bacterial strain known passes through routine culture, the microbial inoculum of the numerous preparation of expansion.
CN201810660404.1A 2018-06-25 2018-06-25 A kind of defatted rice bran Multi-strain fermentation technology Pending CN108850549A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101161113A (en) * 2007-10-29 2008-04-16 大连工业大学 Abstraction of pine nut oil, residual oil cake food after abstracting and its preparing method
CN103976133A (en) * 2013-12-18 2014-08-13 中国农业大学 Cashew nut protein powder and preparation method thereof
CN105639390A (en) * 2015-12-19 2016-06-08 铁力市四宝生物科技有限公司 Nutlet electuary and preparation technology thereof
CN105886423A (en) * 2016-01-20 2016-08-24 江苏盐城源耀生物科技有限公司 Preparation method of biofermentation rice bran meal
CN106805182A (en) * 2017-02-28 2017-06-09 钱灿星 One kind fills blood beauty treatment walnut powder and preparation method thereof
CN107198015A (en) * 2017-04-24 2017-09-26 杭州研青生物科技有限公司 The method that double bacterium semidry method fermentation defatted rice brans obtain active matter

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101161113A (en) * 2007-10-29 2008-04-16 大连工业大学 Abstraction of pine nut oil, residual oil cake food after abstracting and its preparing method
CN103976133A (en) * 2013-12-18 2014-08-13 中国农业大学 Cashew nut protein powder and preparation method thereof
CN105639390A (en) * 2015-12-19 2016-06-08 铁力市四宝生物科技有限公司 Nutlet electuary and preparation technology thereof
CN105886423A (en) * 2016-01-20 2016-08-24 江苏盐城源耀生物科技有限公司 Preparation method of biofermentation rice bran meal
CN106805182A (en) * 2017-02-28 2017-06-09 钱灿星 One kind fills blood beauty treatment walnut powder and preparation method thereof
CN107198015A (en) * 2017-04-24 2017-09-26 杭州研青生物科技有限公司 The method that double bacterium semidry method fermentation defatted rice brans obtain active matter

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Application publication date: 20181123