CN108850549A - A kind of defatted rice bran Multi-strain fermentation technology - Google Patents
A kind of defatted rice bran Multi-strain fermentation technology Download PDFInfo
- Publication number
- CN108850549A CN108850549A CN201810660404.1A CN201810660404A CN108850549A CN 108850549 A CN108850549 A CN 108850549A CN 201810660404 A CN201810660404 A CN 201810660404A CN 108850549 A CN108850549 A CN 108850549A
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- Prior art keywords
- rice bran
- defatted rice
- strain
- composite bacteria
- fermentation
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Links
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 64
- 235000009566 rice Nutrition 0.000 title claims abstract description 64
- 238000000855 fermentation Methods 0.000 title claims abstract description 31
- 230000004151 fermentation Effects 0.000 title claims abstract description 31
- 238000005516 engineering process Methods 0.000 title claims abstract description 18
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 63
- 241000894006 Bacteria Species 0.000 claims abstract description 30
- 239000002131 composite material Substances 0.000 claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 244000168141 Geotrichum candidum Species 0.000 claims abstract description 11
- 235000017388 Geotrichum candidum Nutrition 0.000 claims abstract description 11
- 241000194020 Streptococcus thermophilus Species 0.000 claims abstract description 11
- 241000223260 Trichoderma harzianum Species 0.000 claims abstract description 11
- 238000001035 drying Methods 0.000 claims abstract description 4
- 239000002054 inoculum Substances 0.000 claims abstract description 4
- 230000001954 sterilising effect Effects 0.000 claims abstract description 3
- 239000000203 mixture Substances 0.000 claims description 9
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 238000009472 formulation Methods 0.000 claims description 3
- 239000002068 microbial inoculum Substances 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 241000223259 Trichoderma Species 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 abstract description 10
- 108090000623 proteins and genes Proteins 0.000 abstract description 10
- 238000000034 method Methods 0.000 abstract description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 6
- 241001465754 Metazoa Species 0.000 abstract description 5
- 239000000835 fiber Substances 0.000 abstract description 3
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 3
- 150000001413 amino acids Chemical class 0.000 abstract description 2
- -1 small peptide Chemical compound 0.000 abstract description 2
- 235000018102 proteins Nutrition 0.000 description 9
- 235000015097 nutrients Nutrition 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- 239000006227 byproduct Substances 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 230000000050 nutritive effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 108010059892 Cellulase Proteins 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 229940106157 cellulase Drugs 0.000 description 2
- 238000005238 degreasing Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 108010064851 Plant Proteins Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 235000019774 Rice Bran oil Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 108010050181 aleurone Proteins 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000021329 brown rice Nutrition 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 235000020247 cow milk Nutrition 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000021118 plant-derived protein Nutrition 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000008165 rice bran oil Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/249—Thermophilus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The present invention relates to a kind of defatted rice bran Multi-strain fermentation technologies, include the following steps:(1) defatted rice bran is crushed to 40-80 mesh, 100-120 degrees Celsius sterilizing 10-30 seconds;(2) pure water is added into treated defatted rice bran, water content is set to reach 20-40%, composite bacteria is accessed thereto, the mass percent that inoculum concentration accounts for defatted rice bran is 0.5%-1.5%, control temperature 30-40 degrees Celsius fermentation 12-24 hours, the composite bacteria is by trichodermaharzianum, geotrichum candidum, streptococcus thermophilus according to 2-3:1-2:The ratio of 1-2 forms;(3) product after fermentation is lower than 12% in 40-55 degrees Celsius of drying of temperature to water content, defatted rice bran tunning is made after crushing.The animals such as the nonprotein nitrogen (such as small peptide, free amino acid) of defatted rice bran can be improved by composite bacteria fermentation technique by the present invention can utilize the content of protein sources, reduce crude fiber level, and improve polyoses content.
Description
Technical field
The invention belongs to technical field of biological fermentation, more particularly, to a kind of defatted rice bran Multi-strain fermentation technology.
Background technique
For the annual paddy total output in China at 200,000,000 tons or so, by-product of the rice bran as Rice producing is annual to produce about 1800
Ten thousand tons of rice bran accounts for about the 10% of brown rice weight.Rice bran is mainly made of kind of a skin, megarchidium layer, aleurone and embryo.Due in rice bran
Containing the environmental contaminants in various enzymes and mill processes, it quickly can souring corruption.Therefore, most of rice bran can be by honest and clean at once
Valence is sold as feed or fertilizer.Although there are these disadvantages for rice bran, due to rice bran nutrition rich in (sugar
33.5-53.5%, protein 11.5-17.2%, grease 12.8-22.6% and minerals and vitamin etc.), also it is rich in phenol
The active components of plants such as substance are a kind of " nutrient sources of being bestowed by heaven ".
Defatted rice bran is using the byproduct of rice bran production rice bran oil, and skimming processes make the lipase in rice bran lose work
Property, the undesirable substances such as fungi, the bacterium in rice bran are eliminated, the natural nutrient component of rice bran is remained.Most domestic at present
Defatted rice bran is only used as feedstuff use, and added value is lower, and protein component therein fails to be reprocessed and integrated benefit
With.Rice bran protein is to generally acknowledge quality plant protein, and essential amino acid Compositional balance is reasonable, close to FAO/WHO recommendation pattern;
Wherein lysine content is higher, is that other vegetable proteins are incomparable, and rice bran protein biological value is very high, and nutritive value can be with
Egg, cow's milk compare favourably, and digestibility is up to 90% or more.Rice bran protein is mainly by albumin, globulin, alcohol soluble protein and paddy
Four part of albumen composition, rice bran protein affect dissolubility in aqueous solution, belong to molten because itself containing more disulfide bond
The lower protein of solution property.
It is increasingly taken seriously, can be comprehensively utilized using reasonable technique to mention to the comprehensive utilization of rice bran resource
Its added value of height.By the research to defatted rice bran Multi-strain fermentation technology, can be applied in rice by-product deep processing, energy
Enough significantly improve the nutritive value and biology availability of defatted rice bran.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of defatted rice bran Multi-strain fermentation technology, it can be used for rice pair
In product deep processing, the nutritive value and biology availability of defatted rice bran can be significantly improved.
The present invention is achieved by the following technical programs.
The present invention provides a kind of defatted rice bran Multi-strain fermentation technology, which is characterized in that includes the following steps:
(1) defatted rice bran is crushed to 40-80 mesh, 100-120 degrees Celsius sterilizing 10-30 seconds;
(2) pure water is added into treated defatted rice bran, so that water content is reached 20-40%, accesses thereto compound
Strain, the mass percent that inoculum concentration accounts for defatted rice bran is 0.5%-1.5%, controls temperature in 30-40 degrees Celsius of fermentation 12-24
Hour, the composite bacteria is by trichodermaharzianum, geotrichum candidum, streptococcus thermophilus according to 2-3:1-2:The ratio of 1-2 forms;
(3) product after fermentation is lower than 12% in 40-55 degrees Celsius of drying of temperature to water content, degreasing is made after crushing
Rice bran tunning.
Further, the composite bacteria is by trichodermaharzianum, geotrichum candidum, streptococcus thermophilus according to 2:1:1 ratio group
At.
Further, the number of viable of each strain is respectively in the composite bacteria:Trichodermaharzianum not less than 1 ×
107Cfu/mL, geotrichum candidum are not less than 1 × 107Cfu/mL, streptococcus thermophilus are not less than 1 × 108cfu/mL。
Further, in the composite bacteria single culture used from each strain commercially available single active bacteria formulation, or
Person passes through routine culture, the microbial inoculum of the numerous preparation of expansion using any known bacterial strain for being accredited as corresponding strain through microbiology.
Compared with prior art, the beneficial effect that the present invention obtains includes:
Nonprotein nitrogen (such as the small peptide, free ammonia of defatted rice bran can be improved by composite bacteria fermentation technique by the present invention
Base acid) etc. animals can utilize the contents of protein sources, reduce crude fiber level, and improve polyoses content, to be inhaled for animal digestion
It receives nutrient therein and provides good basis.Also, by comparison, it was found that the composite bacteria being made of three kinds of strains relative to
The combination of single culture or two kinds of strains achieves more significant progress effect, shows that these three strains are de- for fermenting
There is synergistic effect, it may be possible to since these three strains can promote mutually bacterial activity to more in fermentation for rouge rice bran
It is related that the biodegradable enzymes such as protease, cellulase, pectase are generated at a high level.
Specific embodiment
Technical solution of the present invention is described further below by specific embodiment, but following specific embodiments do not have to
In the limitation present invention.It is all within spirit of that invention and principle, any modification, equivalent replacement, improvement and so on should all include
Within protection scope of the present invention.
Embodiment 1
A kind of defatted rice bran Multi-strain fermentation technology, which is characterized in that include the following steps:
(1) defatted rice bran is crushed to 60 mesh, 110 degrees Celsius sterilize 20 seconds;
(2) pure water is added into treated defatted rice bran, so that water content is reached 30%, accesses compound bacteria thereto
Kind, the mass percent that inoculum concentration accounts for defatted rice bran is 1.0%, and control temperature is fermented 18 hours at 35 degrees Celsius, described compound
Strain is by trichodermaharzianum, geotrichum candidum, streptococcus thermophilus according to 2:1:1 ratio composition;
(3) product after fermentation is lower than 12% in 50 degrees Celsius of dryings of temperature to water content, degreasing rice is made after crushing
Chaff tunning.
The number of viable of each strain is respectively in the composite bacteria:Trichodermaharzianum is not less than 1 × 107Cfu/mL, Bai Di
It is mould to be not less than 1 × 107Cfu/mL, streptococcus thermophilus are not less than 1 × 108cfu/mL;Single culture used is next in the composite bacteria
From in the commercially available single active bacteria formulation of each strain.
Embodiment 2
A kind of defatted rice bran Multi-strain fermentation technology, other than composite bacteria used only includes trichodermaharzianum, other steps
Suddenly embodiment 1 is same as with parameter.
Embodiment 3
A kind of defatted rice bran Multi-strain fermentation technology, other than composite bacteria used only includes geotrichum candidum, other steps
Embodiment 1 is same as with parameter.
Embodiment 4
A kind of defatted rice bran Multi-strain fermentation technology, other than composite bacteria used only includes streptococcus thermophilus, other
Step and parameter are same as embodiment 1.
Embodiment 5
A kind of defatted rice bran Multi-strain fermentation technology, in addition to composite bacteria used is by trichodermaharzianum, geotrichum candidum according to 2:1
Ratio composition except, other steps and parameter are same as embodiment 1.
Embodiment 6
A kind of defatted rice bran Multi-strain fermentation technology, in addition to composite bacteria used be by trichodermaharzianum, streptococcus thermophilus by
According to 2:Except 1 ratio composition, other steps and parameter are same as embodiment 1.
Embodiment 7
A kind of defatted rice bran Multi-strain fermentation technology, in addition to composite bacteria used be by geotrichum candidum, streptococcus thermophilus according to
1:Except 1 ratio composition, other steps and parameter are same as embodiment 1.
Embodiment 8
Using defatted rice bran made from embodiment 1-7 as 7 test groups, as a control group by raw material defatted rice bran, measurement
Nutrient content in this 8 groups of samples, measuring method is using nutrient measuring method well known to field of fodder.Measurement result is referring to following table
1。
As shown in Table 1, the nonprotein nitrogen (such as small peptide, free amino acid) of defatted rice bran can be improved by strain fermentation
Equal animals can utilize the content of protein sources, reduce crude fiber level, and improve polyoses content, to absorb wherein for animal digestion
Nutrient provide good basis.Also, by comparison, it was found that the composite bacteria being made of three kinds of strains is relative to single bacterium
The combination of kind or two kinds of strains achieves more significant progress effect, shows these three strains for the defatted rice bran that ferments
For there is synergistic effect, it may be possible to since these three strains can promote mutually bacterial activity to higher level in fermentation
The biodegradable enzymes such as real estate raw albumen enzyme, cellulase, pectase are related.
Concrete scheme and main feature of the invention has been shown and described above, and to a certain extent by test data
Embody advantages of the present invention.It should be understood by those skilled in the art that the present invention is not limited to the above embodiments, above-mentioned reality
It applies and only illustrates concrete scheme of the invention described in example and specification, in the premise for not departing from spirit and scope of the invention
Under, various changes and improvements may be made to the invention, and these changes and improvements all fall within the protetion scope of the claimed invention.This hair
Bright claimed range is defined by the appending claims and its equivalent thereof.
Claims (4)
1. a kind of defatted rice bran Multi-strain fermentation technology, which is characterized in that include the following steps:
(1) defatted rice bran is crushed to 40-80 mesh, 100-120 degrees Celsius sterilizing 10-30 seconds;
(2) pure water is added into treated defatted rice bran, so that water content is reached 20-40%, accesses composite bacteria thereto,
Inoculum concentration account for defatted rice bran mass percent be 0.5%-1.5%, control temperature 30-40 degrees Celsius fermentation 12-24 hours,
The composite bacteria is by trichodermaharzianum, geotrichum candidum, streptococcus thermophilus according to 2-3:1-2:The ratio of 1-2 forms;
(3) product after fermentation is lower than 12% in 40-55 degrees Celsius of drying of temperature to water content, defatted rice bran is made after crushing
Tunning.
2. defatted rice bran Multi-strain fermentation technology as described in claim 1, which is characterized in that the composite bacteria is by healthy and free from worry
Trichoderma, geotrichum candidum, streptococcus thermophilus are according to 2:1:1 ratio composition.
3. defatted rice bran Multi-strain fermentation technology as described in claim 1, which is characterized in that each strain in the composite bacteria
Number of viable be respectively:Trichodermaharzianum is not less than 1 × 107Cfu/mL, geotrichum candidum are not less than 1 × 107Cfu/mL, thermophilus
Bacterium is not less than 1 × 108cfu/mL。
4. defatted rice bran Multi-strain fermentation technology as described in claim 1, which is characterized in that list used in the composite bacteria
One strain from each strain commercially available single active bacteria formulation, or using through microbiology be accredited as corresponding strain it is any
The bacterial strain known passes through routine culture, the microbial inoculum of the numerous preparation of expansion.
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CN201810660404.1A CN108850549A (en) | 2018-06-25 | 2018-06-25 | A kind of defatted rice bran Multi-strain fermentation technology |
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CN101161113A (en) * | 2007-10-29 | 2008-04-16 | 大连工业大学 | Abstraction of pine nut oil, residual oil cake food after abstracting and its preparing method |
CN103976133A (en) * | 2013-12-18 | 2014-08-13 | 中国农业大学 | Cashew nut protein powder and preparation method thereof |
CN105639390A (en) * | 2015-12-19 | 2016-06-08 | 铁力市四宝生物科技有限公司 | Nutlet electuary and preparation technology thereof |
CN105886423A (en) * | 2016-01-20 | 2016-08-24 | 江苏盐城源耀生物科技有限公司 | Preparation method of biofermentation rice bran meal |
CN106805182A (en) * | 2017-02-28 | 2017-06-09 | 钱灿星 | One kind fills blood beauty treatment walnut powder and preparation method thereof |
CN107198015A (en) * | 2017-04-24 | 2017-09-26 | 杭州研青生物科技有限公司 | The method that double bacterium semidry method fermentation defatted rice brans obtain active matter |
-
2018
- 2018-06-25 CN CN201810660404.1A patent/CN108850549A/en active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101161113A (en) * | 2007-10-29 | 2008-04-16 | 大连工业大学 | Abstraction of pine nut oil, residual oil cake food after abstracting and its preparing method |
CN103976133A (en) * | 2013-12-18 | 2014-08-13 | 中国农业大学 | Cashew nut protein powder and preparation method thereof |
CN105639390A (en) * | 2015-12-19 | 2016-06-08 | 铁力市四宝生物科技有限公司 | Nutlet electuary and preparation technology thereof |
CN105886423A (en) * | 2016-01-20 | 2016-08-24 | 江苏盐城源耀生物科技有限公司 | Preparation method of biofermentation rice bran meal |
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Application publication date: 20181123 |