CN108849257A - A kind of Cordyceps militaris science cultural method - Google Patents
A kind of Cordyceps militaris science cultural method Download PDFInfo
- Publication number
- CN108849257A CN108849257A CN201811121479.9A CN201811121479A CN108849257A CN 108849257 A CN108849257 A CN 108849257A CN 201811121479 A CN201811121479 A CN 201811121479A CN 108849257 A CN108849257 A CN 108849257A
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- Prior art keywords
- culture
- cordyceps militaris
- strain
- cultural method
- culture medium
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention discloses a kind of Cordyceps militaris science cultural method, includes the following steps：Good quality strain is selected to be inoculated with, the culture sill of needs is mixed and is configured to culture medium, inorganic selenium is added into culture medium, and phosphate, then it after culture medium being placed at 110 DEG C of temperature the 15-20min that sterilizes, is cooled to room temperature, on the culture medium after the original strain of selection to be inoculated into sterilization treatment, inoculated culture bottle is placed in dark culture 6-10d under the conditions of 22-25 DEG C of temperature, humidity 40 one 55% produce, until mycelium covers with entire media surface.A kind of Cordyceps militaris science cultural method of the present invention, easy to operate, sterilization effect is good, improves the growth rate of strain.
Description
Technical field
The present invention relates to cordyceps sinensis to cultivate field, more particularly to a kind of Cordyceps militaris science cultural method.
Background technique
Cordyceps militaris, also known as northern Chinese caterpillar Fungus, Cordceps militaris is Ascomycotina, ergot Zoopagales, Clavicipitaceae, Cordyceps
Type sepecies are to belong to not of the same race and a kind of medicinal fungi having long enjoyed a good reputation with cordyceps sinensis.Can all herbal medicine, have it is special
Medical value and nourishing effects.Contain cordycepic acid, cordycepin, Cordyceps sinensis polysaccharide, superoxide dismutase SOD and trace elements of selenium
Equal various bioactivators, can promote body metabolism, improve the immune function of body, using traditional artificial cultivation, be
Cordyceps Militaris is injected into silkworm chrysalis living, simulating natural condition obtains Cordyceps militaris.But it cultivates in this way, injection technique
It is difficult to control, is easy pollution, low output, general peasant household is difficult to operate.
Summary of the invention
It is easy to operate the invention mainly solves the technical problem of providing a kind of Cordyceps militaris science cultural method, sterilizing effect
Fruit is good, improves the growth rate of strain.
In order to solve the above technical problems, one technical scheme adopted by the invention is that：A kind of Cordyceps militaris science cultural method,
Include the following steps：
1）The selection of bacterial strain：Good quality strain is selected to be inoculated with；
2）Prepare culture medium：The culture sill of needs is mixed and is configured to culture medium, is added into culture medium inorganic
Selenium and phosphate are cooled to room temperature after culture medium is then placed at 110 DEG C of temperature the 15-20min that sterilizes；
3）The activation of strain：On culture medium after the original strain of selection to be inoculated into sterilization treatment, mixing time 20-
25min；
4）Parent species cultivation：Inoculated culture bottle is placed in dark culture 6- under the conditions of 22-25 DEG C of temperature, humidity 40 one 55% produce
10d, until mycelium covers with entire media surface.
In one embodiment of the invention, the culture sill is by more property albumen navel 10g, glucose 50g, phosphoric acid hydrogen
Dipotassium 1g, magnesium sulfate 0.5g, silkworm chrysalis 30g living, auxin 0.5 μ g, agar 20g add water 1000mL to be mixed.
In one embodiment of the invention, the culture sill removes the peel fresh Ma Ling by albumen dried meat 40g, glucose 40g
Potato 100g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.5g, beef extract 10g, 0.5 μ g of auxin, bat moth larva living (grind) 30g, fine jade
Rouge 20g, Alpine Meadow leachate 10OOmL are mixed.
In one embodiment of the invention, the inorganic selenium is selenite.
In one embodiment of the invention, the phosphate includes disodium-hydrogen, one of sodium dihydrogen phosphate or
Two kinds.
In one embodiment of the invention, when strain inoculated, combustible medium is enclosed in the bottle mouth position setting one for being put into strain,
It lights combustible medium and forms sealing ring.
The beneficial effects of the invention are as follows：A kind of Cordyceps militaris science cultural method of the present invention, easy to operate, sterilization effect is good,
Improve the growth rate of strain.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiments of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's all other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
The embodiment of the present invention includes：
In order to solve the above technical problems, one technical scheme adopted by the invention is that：A kind of Cordyceps militaris science cultural method, packet
Include following steps：
1）The selection of bacterial strain：Good quality strain is selected to be inoculated with；
2）Prepare culture medium：The culture sill of needs is mixed and is configured to culture medium, is added into culture medium inorganic
Selenium and phosphate are cooled to room temperature after culture medium is then placed at 110 DEG C of temperature the 15-20min that sterilizes；
3）The activation of strain：On culture medium after the original strain of selection to be inoculated into sterilization treatment, mixing time 20-
25min；
4）Parent species cultivation：Inoculated culture bottle is placed in dark culture 6- under the conditions of 22-25 DEG C of temperature, humidity 40 one 55% produce
10d, until mycelium covers with entire media surface.
Among the above, the culture sill is by more property albumen navel 10g, glucose 50g, dipotassium hydrogen phosphate 1g, magnesium sulfate
0.5g, silkworm chrysalis 30g living, auxin 0.5 μ g, agar 20g add water 1000mL to be mixed.
Among the above, the culture sill removes the peel fresh potato 100g, phosphoric acid hydrogen two by albumen dried meat 40g, glucose 40g
Potassium 1g, magnesium sulfate 0.5g, beef extract 10g, 0.5 μ g of auxin, bat moth larva living (grind) 30g, agar 20g, Alpine Meadow
Leachate 10OOmL is mixed.
Among the above, the inorganic selenium is selenite.
Among the above, the phosphate includes one or both of disodium-hydrogen, sodium dihydrogen phosphate.
Among the above, it when strain inoculated, in the one circle combustible medium of bottle mouth position setting for being put into strain, lights combustible medium and is formed
Sealing ring.
The beneficial effect of Cordyceps militaris science cultural method of the present invention is：A kind of Cordyceps militaris science cultural method of the present invention, behaviour
Make simply, sterilization effect is good, improves the growth rate of strain.
The above description is only an embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair
Equivalent structure or equivalent flow shift made by bright description is applied directly or indirectly in other relevant technology necks
Domain is included within the scope of the present invention.
Claims (6)
1. a kind of Cordyceps militaris science cultural method, which is characterized in that include the following steps：
The selection of bacterial strain：Good quality strain is selected to be inoculated with；
Prepare culture medium：The culture sill of needs is mixed and is configured to culture medium, inorganic selenium is added into culture medium,
And phosphate is cooled to room temperature after culture medium is then placed at 110 DEG C of temperature the 15-20min that sterilizes；
The activation of strain：On culture medium after the original strain of selection to be inoculated into sterilization treatment, mixing time 20-25min；
Parent species cultivation：Inoculated culture bottle is placed in dark culture 6-10d under the conditions of 22-25 DEG C of temperature, humidity 40 one 55% produce,
Until mycelium covers with entire media surface.
2. Cordyceps militaris science cultural method according to claim 1, which is characterized in that the culture sill is by more property
Albumen navel 10g, glucose 50g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.5g, silkworm chrysalis 30g living, auxin 0.5 μ g, agar 20g add
Water 1000mL is mixed.
3. Cordyceps militaris science cultural method according to claim 1, which is characterized in that the culture sill is by albumen
Dried meat 40g, glucose 40g remove the peel fresh potato 100g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.5g, beef extract 10g, 0.5 μ of auxin
G, bat moth larva living (grind) 30g, agar 20g, and Alpine Meadow leachate 10OOmL is mixed.
4. Cordyceps militaris science cultural method according to claim 1, which is characterized in that the inorganic selenium is selenite.
5. Cordyceps militaris science cultural method according to claim 1, which is characterized in that the phosphate includes phosphoric acid one
Hydrogen sodium, one or both of sodium dihydrogen phosphate.
6. Cordyceps militaris science cultural method according to claim 1, which is characterized in that when strain inoculated, be put into strain
Bottle mouth position setting one circle combustible medium, light combustible medium formed sealing ring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811121479.9A CN108849257A (en) | 2018-09-26 | 2018-09-26 | A kind of Cordyceps militaris science cultural method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811121479.9A CN108849257A (en) | 2018-09-26 | 2018-09-26 | A kind of Cordyceps militaris science cultural method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108849257A true CN108849257A (en) | 2018-11-23 |
Family
ID=64324658
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811121479.9A Pending CN108849257A (en) | 2018-09-26 | 2018-09-26 | A kind of Cordyceps militaris science cultural method |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104164367A (en) * | 2014-08-01 | 2014-11-26 | 沁阳市西向食用菌研究所 | Dried silkworm cordyceps militaris and culture method thereof |
| CN105838624A (en) * | 2016-04-26 | 2016-08-10 | 贵州省烟草公司贵阳市公司 | Cordycepsmilitaris(L.)Link fruiting body culture method capable of preventing mixed fungus pollution |
| CN106191173A (en) * | 2016-07-21 | 2016-12-07 | 内江师范学院 | A kind of method improving Cordyceps militaris fermenting and producing yield of Cordycepin |
| CN107604026A (en) * | 2017-10-18 | 2018-01-19 | 甘肃省科学院生物研究所 | A kind of method for improving cordyceps liquid fermentation cordycepin output |
| CN107603888A (en) * | 2017-10-10 | 2018-01-19 | 南通市新丝路蚕业有限公司 | A kind of producing method for seed of Cordyceps militaris strain |
-
2018
- 2018-09-26 CN CN201811121479.9A patent/CN108849257A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104164367A (en) * | 2014-08-01 | 2014-11-26 | 沁阳市西向食用菌研究所 | Dried silkworm cordyceps militaris and culture method thereof |
| CN105838624A (en) * | 2016-04-26 | 2016-08-10 | 贵州省烟草公司贵阳市公司 | Cordycepsmilitaris(L.)Link fruiting body culture method capable of preventing mixed fungus pollution |
| CN106191173A (en) * | 2016-07-21 | 2016-12-07 | 内江师范学院 | A kind of method improving Cordyceps militaris fermenting and producing yield of Cordycepin |
| CN107603888A (en) * | 2017-10-10 | 2018-01-19 | 南通市新丝路蚕业有限公司 | A kind of producing method for seed of Cordyceps militaris strain |
| CN107604026A (en) * | 2017-10-18 | 2018-01-19 | 甘肃省科学院生物研究所 | A kind of method for improving cordyceps liquid fermentation cordycepin output |
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Application publication date: 20181123 |