CN108841838A - A kind of neomorph and its application controlling rice low amylose content - Google Patents
A kind of neomorph and its application controlling rice low amylose content Download PDFInfo
- Publication number
- CN108841838A CN108841838A CN201810743434.9A CN201810743434A CN108841838A CN 108841838 A CN108841838 A CN 108841838A CN 201810743434 A CN201810743434 A CN 201810743434A CN 108841838 A CN108841838 A CN 108841838A
- Authority
- CN
- China
- Prior art keywords
- rice
- gene
- seq
- round
- chu
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Nutrition Science (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of content of amylose in rice neomorph Wxc39, belong to field of biotechnology.The gene contains the gene order as shown in SEQ ID No.1 and the coding region sequence as shown in SEQ ID No.2 and SEQ ID No.3 in the 129th to 139 nucleotide deletion of the gene coding region hydratase gene rice Wx exon 2.The invention also discloses a kind of Wxc39Encoded albumen contains the amino acid sequence as shown in SEQ ID No.4.Amylose content control gene of the invention can also be applied to the breeding of low amylose new rice variety.
Description
One, technical field
The present invention relates to a kind of neomorph Wx for controlling rice low amylose contentC39And its application, belong to molecule
Genetic arts.
Two, background technique
Amylose content is the important factor in order of rice cooking and eating quality.In general, amylose content
20% or more rice variety poor eating, amylose food flavor below 15%~20% are preferable.In recent years, amylose content
Between 5%~12% soft rice, due to its rice have it is soft without it is rotten, sweet it is tasty and refreshing, puffing is good, high resilience, it is cold after not
It is easily hardened, the advantages that retrogradation degree is small, and there are extensive market (Zhang Changquan etc., Scientia Agricultura Sinica, 2016,49 (22):
4267-4283)。
The soft rice plantation in China is distributed mainly on southwest, especially Yunnan.Soft rice product are just planted in history in Yunnan Province
Kind, character gene is in wild rice caused by natural variation, and is retained by the food custom of the local common people.Wherein with
The milli people, milli wind are Typical Representative, are the good merchantable brands paid tribute to imperial court in the successive dynasties, are after liberation listed in the high-quality first-class rice of country, commonly use with
It entertains distinguished guests (Liu et al., Plant Molecular Biology, 2009,71 (6):609-626).In Yunnan, cultivate
A series of high-quality soft rice kinds such as silver-colored light, cloud round-grained rice 20, cloud round-grained rice 29, No. 4 excellent, the Chu's round-grained rice 39 of cloud round-grained rice.According to statistics, China in recent years
Soft rice is mainly distributed on southwest, accounts for the 53% of the soft rice total amount in China, followed by Central China and South China, East China, northeast, China
The ground such as north, northwest cultivated area is less.As Jiangsu, northeast are to the pay attention to day by day of soft rice breeding, development, it is contemplated that East China and northeast
The area in area will increase dramatically, and shared ratio can also increase.
Chu's round-grained rice 39 is the soft rice material that we obtain from Yunnan Screening germplasm, and the kind is at present in the production of Yunnan
It promotes and applies.The rice apparent haziness milky of Chu's round-grained rice 39, research finds that frameshit occurs for the Wx gene of the kind through the invention
Mutation, but amylose content still is able to reach 5% or so, and rice matter belongs to soft rice type between glutinous rice and glutinous rice.Benefit
With new soft rice resource, new low amylose content controlling gene is found, it is soft for enriching soft rice controlling gene library and parsing
Rice Forming Mechanism has great theoretical and practical significance.
Three, summary of the invention
Technical problem：
The present invention is intended to provide a kind of neomorph and its application for controlling rice low amylose content, can control
It is applied in rice low amylose content breeding.
Technical solution：
The present invention provides a kind of content of amylose in rice related gene Wxc39, it is EU770319 in gene accession number
The gene coding region rice Waxy exon 2 the 129th to 139 nucleotide deletion, contain as shown in SEQ ID No.1
Gene order and the coding region sequence as shown in SEQ ID No.2.
The invention also discloses a kind of Wxc39Encoded albumen contains the amino acid sequence as shown in SEQ ID No.3
Column.
Gene of the present invention can be applied in rice varieties improvement.
Beneficial effect：
There has been no the reports of the Waxy gene allelic variation at present.The research of the invention finds that Chu's round-grained rice 39 is in gene accession number
The nucleotide deletion (Fig. 3) of gene coding region Waxy (Wx) the 129th to 139 of EU770319, sequence such as SEQ ID NO.1 institute
Show.The mutation causes the translation frameshift of transcription product, also results in protein translation and terminates in advance.Therefore, the Waxy of Chu's round-grained rice 39
Gene is in WxbThe new allelic variation occurred in type basis, is named as Wxc39。
The present invention provides a kind of relevant protein of content of amylose in rice and its encodes the gene Wx of the proteinc39。
The gene encodes one and combines the starch synthase gene GBSS I on amylum body, is the major regulatory base of amylose content
Cause plays key effect to the cooking and eating quality of rice.Carry Wxc39The rice plant of homozygous genotype, amylose content
5% or so, belong to soft rice type.
Research finds that frameshift mutation occurs for the Wx gene of the kind through the invention, but amylose content still is able to reach
To 5% or so, rice matter belongs to soft rice type between glutinous rice and glutinous rice.Using new soft rice resource, new low straight chain is found
Content of starch controlling gene has important theory and practice for enriching soft rice controlling gene library and the soft rice Forming Mechanism of parsing
Meaning.
Utilize the tied F of cenospecies of Chu's round-grained rice 39 and Su Ken 1182Germination extracts blade genome, to part single plant
Wx gene carries out sequencing discovery, shows as milky single plant and all carries Wxc39Wxc39Genotype shows as translucent single plant
Carry WxbWxc39And WxbWxbTwo kinds of genotype, Wxc39Gene isolates completely with endosperm milky phenotype.Utilize hybridization, backcrossing
Etc. conventional technical means can cultivate new soft rice material by the gene transformation into other rice varieties.
Four, Detailed description of the invention
Fig. 1 rice appearance diagram
Fig. 2 rice cross section I2- KI dyeing
A is the sectional view that is unstained, B I2Sectional view after-KI dyeing
Fig. 3 Wx gene order compares
Nipponbare is OryzasativaLcv.Nipponbare, and Chujing39 is Chu's round-grained rice 39
The Endosperm appaearance phenotype of Fig. 4 different generations
Fig. 5 Wxc39Expression of the allele in different tissues
White columns indicate OryzasativaLcv.Nipponbare, and black bar indicates Chu's round-grained rice 39
Five, specific embodiment
Embodiment of the present invention is described in detail below in conjunction with specific implementation step.It is not specified in implementation steps
Actual conditions person, the condition that routinely condition or manufacturer suggest carry out.Production firm person is not specified in agents useful for same and instrument,
For the conventional products obtained can be bought by market.Specific implementation step is as follows：
1, test material
Suyunuo, Chu's round-grained rice 39, southern round-grained rice 9108 and Su Ken 118 are public material, Jiangsu Province's agricultural germplasm resource bank
It can provide free.Chu's round-grained rice 39 is the soft rice material that we obtain from Yunnan Screening germplasm.Particular reference is：Wu Jinglun
Deng, Jiangsu's agriculture science, 1992, (3):6-8；Xu plus equality, agricultural science and technology communication, 2016, (05):167-1；Wang Cailin etc.,
Jiangsu's agriculture science, 2013,41 (09):86-88；Yang Jie etc., Jiangsu's agriculture science, 2017,45 (21):79-81.
2, rice appearance and amylose content determination
Seed is removed into glume and aleurone, discovery 39 rice of Chu's round-grained rice is opaque, muddy milky is presented, close to glutinous rice
Kind Su Yu glutinous (Fig. 1), 118 rice of Su Ken are translucent.The I of rice2The discovery of-KI coloration result, glutinous rice variety Suyunuo dye
It is brown after color, and darkviolet (Fig. 2) is presented in Chu's round-grained rice 39, southern round-grained rice 9108 and Su Ken 118.Further compare Chu's round-grained rice 39, glutinous rice,
Soft rice and the discovery of the amylose content of japonica rice, amylose content of the Chu's round-grained rice 39 in Nanjing and Chuxiong is respectively 4.5% He
4.7%, it is higher than glutinous rice variety Suyunuo (amylose content 2.7%), lower than soft rice kind south round-grained rice 9108, (amylose contains
9.3%) and japonica rice variety Su Ken 118 (amylose content 13.9%) (table 1) amount is.Therefore, Chu's round-grained rice 39 belongs to low straight chain
Content of starch kind, in 39 amylose content of Chu's round-grained rice that Yunnan and Jiangsu Province are planted in 4-5%.
1 amylose content of table
3, genetic analysis
It is female parent with Chu's round-grained rice 39, Su Ken 118 is the combination of male parent preparing hybrid, harvests F0；By F0After plantation, seed F is harvested1,
Observe F0And F1Endosperm phenotype.F0Endosperm be it is translucent, show it is translucent relative to milky be it is dominant.To 498 F1Harvest kind
Son endosperm carry out phenotypic evaluation, discovery 370 show as it is translucent, discovery 128 show as milky, through Chi-square Test
(χ2=0.0825<χ2 0.05,1), it is translucent to meet 3 with milky segregation ratio:1, show 39 endosperm of Chu's round-grained rice milky phenotype be by
The recessive character of single karyogene control.
4, gene cloning
Wx gene is the major gene resistance of adjusting and controlling rice content of starch, we are first sequenced it.It is extracted with CTAB method
The genome of 39 blade of Chu's round-grained rice.Using the genomic DNA of extraction as template, PCR amplification is carried out.Expanding upstream primer used is 5 '-
Cggtgcccaacagaaaccaca-3 ', downstream primer 5 '-cacccagaagagtacaacat-3 '.PCR system is DNA mould
Plate (20ng μ L-1) 2 μ L, 10 × PCRbuffer 2 μ L, MgCl2(5mmol L-1) 2 μ L, dNTP (2mmol L-1) 2 μ L, upstream is drawn
Object (2 μm of ol L-1) 2 μ L, downstream primer (2 μm of ol L-1) 2 μ L, Taq archaeal dna polymerase (5U μ L-1) 0.2 μ L, ddH2O 7.8μL。
Amplification condition is 94 DEG C of 5min；94 DEG C of 30s, 55 DEG C of 30s, 72 DEG C of 4min, 35 circulations；72 DEG C of extension 10min, reaction was completed.
PCR is carried out in EppendorfMastercycle thermal cycler.Amplified production is separated through agarose gel electrophoresis, gel extraction
Purpose band is sent to the sequencing of Nanjing Qing Ke Biotechnology Co., Ltd.Using BioXM2.6 software by 39 sequence of Chu's round-grained rice and data
Gene accession number is that the Wx gene of the OryzasativaLcv.Nipponbare of EU770319 carries out sequence alignment in library, in the Wx gene first for finding Chu's round-grained rice 39
The first base containing son is T, belongs to WxbType.In addition, Chu's round-grained rice 39 is in the nucleotide deletion of the gene coding region Wx the 129th to 139
(Fig. 3), sequence is as shown in SEQ ID NO.1.The mutation causes the translation frameshift of transcription product, also results in protein translation and mentions
Preceding termination.There has been no the reports of the Wx gene allelic variation at present.Therefore, the Wx gene of Chu's round-grained rice 39 is in WxbIn type basis
The new allelic variation occurred, is named as Wxc39。
5、Wxc39Gene is isolated with endosperm milky character
Sequencing result shows that Chu's round-grained rice 39 carries Wxc39Wxc39Genotype, Su Ken 118 carry WxbWxbGenotype.Utilize Chu's round-grained rice
The tied F of cenospecies of 39 and Su Ken 1182Germination extracts blade genome, carries out sequencing hair to the Wx gene of part single plant
It is existing, it shows as milky single plant and all carries Wxc39Wxc39Genotype shows as translucent single plant and carries WxbWxc39With
WxbWxbTwo kinds of genotype, Wxc39Gene isolates completely with endosperm milky phenotype, further confirms Wxc39Gene is Chu's round-grained rice
The controlling gene of 39 endosperm milky characters.Using conventional technical means such as hybridization, backcrossings, by the gene transformation to other rice
In kind, new soft rice material can be cultivated.
6, the Wx of Chu's round-grained rice 39c39Gene transcripts analysis
The RNA in 39 endosperm of Chu's round-grained rice and reverse transcription are extracted into cDNA.Using cDNA as template, PCR amplification is carried out.Used in amplification
Upstream primer is 5 '-ACCATTCCTTCAGTTCTTTGT-3 ', downstream primer 5 '-TCAAGGAGCAGCCACGTTCT-3 '.
PCR system is DNA profiling (20ng μ L-1) 2 μ L, 10 × PCRbuffer 2 μ L, MgCl2(5mmol L-1) 2 μ L, dNTP (2mmol
L-1) 2 μ L, upstream primer (2 μm of ol L-1) 2 μ L, downstream primer (2 μm of ol L-1) 2 μ L, Taq archaeal dna polymerase (5U μ L-1)0.2μ
L, ddH2O 7.8μL.Amplification condition is 94 DEG C of 5min；94 DEG C of 30s, 55 DEG C of 30s, 72 DEG C of 3.5min, 35 circulations；72 DEG C of extensions
10min, reaction was completed.PCR is carried out in Eppendorf Mastercycle thermal cycler.Amplified production is through Ago-Gel
Electrophoretic separation, gel extraction purpose band are sent to the sequencing of Nanjing Qing Ke Biotechnology Co., Ltd.It will using BioXM2.6 software
The cDNA sequence of Chu's round-grained rice 39 is compared with the gene order of Chu round-grained rice 39, and the Wx gene of discovery Chu's round-grained rice 39 can generate two kinds of transcriptions productions
Object, respectively as shown in SEQ ID NO.2 and SEQ ID NO.3.SEQ ID NO.2 transcript overall length is 3118bp, in first
It is not cut off containing son.SEQ ID NO.3 transcript overall length is 1997bp, and First Intron has been sheared.The Wx gene of Chu's round-grained rice 39
Splice mode and reported OryzasativaLcv.Nipponbare (Wxb) identical (Wang etc., Plant Journal, 1995,7 (4):613-622), it cuts
The difference for cutting product is only the missing of 39 the 129th to 139 nucleotide in code area of Chu's round-grained rice.The difference leads to the Wx base of Chu's round-grained rice 39
The translation of cause terminates in advance, and amino acid sequence is as shown in SEQ ID NO.4.
7, the Wx of Chu's round-grained rice 39c39The tissue expression spectrum analysis of gene
The sword-like leave of Chu's round-grained rice 39 and OryzasativaLcv.Nipponbare, the RNA of the endosperm of the young fringe and pustulation period that are not in the milk are extracted, reverse transcription is
CDNA, using OsActin-1 as reference gene, with the expression quantity of quantitative PCR identification Wx gene.As shown in figure 5, in Chu's round-grained rice 39 and day
During this is fine, Wx gene is all the expression quantity highest in endosperm, is approximately 90 times of expression quantity in sword-like leave.Compared with OryzasativaLcv.Nipponbare, Chu
Differential expression of the round-grained rice 39Wx gene in sword-like leave and the endosperm of pustulation period be not significant, and significantly drops in the expression not being in the milk in young fringe
It is low, show that the missing of the 129th to 139 nucleotide does not influence expression quantity of the Wx gene in endosperm.
SEQ ID NO.1
Wxc39Gene DNA：
accattccttcagttctttgtctatctcaagacacaaataactgcagtctctctctctctctctctctctctctctc
tctctgcttcacttctctgcttgtgttgttctgttgttcatcaggaagaacatctgcaagttatacatatatgttta
taattctttgtttcccctcttattcagatcgatcacatgcatctttcattgctcgtttttccttacaagtagtctca
tacatgctaatttctgtaaggtgttgggctggaaattaattaattaattaattgacttgccaagatccatatatatg
tcctgatattaaatcttcgttcgttatgtttggttaggctgatcaatgttattctagagtctagagaaacacaccca
ggggttttccaactagctccacaagatggtgggctagctgacctagatttgaagtctcactccttataattatttta
tattagatcattttctaatattcgtgtctttttttattctagagtctagatcttgtgttcaactctcgttaaatcat
gtctctcgccactggagaaacagatcaggagggtttattttgggtataggtcaaagctaagattgaaattcacaaat
agtaaaatcagaatccaaccaattttagtagccgagttggtcaaaggaaaatgtatatagctagatttattgttttg
gcaaaaaaaaatctgaatatgcaaaatacttgtatatctttgtattaagaagatgaaaataagtagcagaaaattaa
aaaatggattatatttcctgggctaaaagaattgttgatttggcacaattaaattcagtgtcaaggttttgtgcaag
aattcagtgtgaaggaatagattctcttcaaaacaatttaatcattcatctgatctgctcaaagctctgtgcatctc
cgggtgcaacggccaggatatttattgtgcagtaaaaaaatgtcatatcccctagccacccaagaaactgctcctta
agtccttataagcacatatggcattgtaatatatatgtttgagttttagcgacaatttttttaaaaacttttggtcc
tttttatgaacgttttaagtttcactgtctttttttttcgaattttaaatgtagcttcaaattctaatccccaatcc
aaattgtaataaacttcaattctcctaattaacatcttaattcatttatttgaaaaccagttcaaattcttttaggc
tcaccaaaccttaaacaattcaattcagtgcagagatcttccacagcaacagctagacaaccaccatgtcggctctc
accacgtcccagctcgccacctcggccaccggcttcggcatcgccgacaggtcggcgccgtcgtcgctgctccgcca
cgggttccagggcctcaagccccgcagccccgccggcggcgctcagcgtgacgaccagcgcgcgcgcgacgcccaag
cagcagcggtcggtgcagcgtggcagccggaggttcccctccgtcgtcgtgtacgccaccggcgccggcatgaacgt
cgtgttcgtcggcgccgagatggccccctggagcaagaccggcggcctcggtgacgtcctcggtggcctcccccctg
ccatggctgtaagcacacacaaacttcgatcgctcgtcgtcgctgaccgtcgtcgtcttcaactgttcttgatcatc
gcattggatggatgtgtaatgttgtgttcttgtgttctttgcaggcgaatggccacagggtcatggtgatctctcct
cggtacgaccagtacaaggacgcttgggataccagcgttgtggctgaggtaggagcatatgcgtgatcagatcatca
caagatcgattagctttagatgatttgttacatttcgcaagattttaacccaagtttttgtggtgcaattcattgca
gatcaaggttgcagacaggtacgagagggtgaggtttttccattgctacaagcgtggagtcgaccgtgtgttcatcg
accatccgtcattcctggagaaggtggagtcatcattagtttaccttttttgtttttactgaattattaacagtgca
tttagcagttggactgagcttagcttccactggtgatttcaggtttggggaaagaccggtgagaagatctacggacc
tgacactggagttgattacaaagacaaccagatgcgtttcagccttctttgccaggtcagtgattacttctatctga
tgatggttggaagcatcacgagtttaccatagtatgtatggattcataactaattcgtgtattgatgctacctgcag
gcagcactcgaggctcctaggatcctaaacctcaacaacaacccatacttcaaaggaacttatggtgagttacaatt
gatctcaagatcttataactttcttcgaaggaatccatgatgatcagactaattccttccggtttgttactgacaac
aggtgaggatgttgtgttcgtctgcaacgactggcacactggcccactggcgagctacctgaagaacaactaccagc
ccaatggcatctacaggaatgcaaaggtctatgcttgttcttgccataccaactcaaatctgcatgcacactgcatt
ctgttcagaaactgactgtctgaatctttttcactgcaggttgctttctgcatccacaacatctcctaccagggccg
tttcgctttcgaggattaccctgagctgaacctctccgagaggttcaggtcatccttcgatttcatcgacgggtatg
agtaagattctaagagtaacttactgtcaattcgccatatatcgattcaatccaagatccttttgagctgacaaccc
tgcactactgtccatcgttcaaatccggttaaatttcaggtatgacacgccggtggagggcaggaagatcaactgga
tgaaggccggaatcctggaagccgacagggtgctcaccgtgagcccgtactacgccgaggagctcatctccggcatc
gccaggggatgcgagctcgacaacatcatgcggctcaccggcatcaccggcatcgtcaacggcatggacgtcagcga
gtgggatcctagcaaggacaagtacatcaccgccaagtacgacgcaaccacggtaagaacgaatgcattcttcacaa
gatatgcaatctgaattttctttgaaaaagaaattatcatctgtcacttcttgattgattctgacaaggcaagaatg
agtgacaaatttcaggcaatcgaggcgaaggcgctgaacaaggaggcgttgcaggcggaggcgggtcttccggtcga
caggaaaatcccactgatcgcgttcatcggcaggctggaggaacagaagggccctgacgtcatggccgccgccatcc
cggagctcatgcaggaggacgtccagatcgttcttctggtataatataatacactacaagacacacttgcacgatat
gccaaaaattcagaacaaattcagtggcaaaaaaaaaactcgaatattagggaaggacctaataatatcaaataatt
agaaggggtgaggctttgaacccagatcgtctagtccaccaccttgtggagttagccggaagacctctgagcatttc
tcaattcagtggcaaatgatgtgtataattttgatccgtgtgtgtttcagggtactggaaagaagaagttcgagaag
ctgctcaagagcatggaggagaagtatccgggcaaggtgagggccgtggtgaagttcaacgcgccgcttgctcatct
catcatggccggagccgacgtgctcgccgtccccagccgcttcgagccctgtggactcatccagctgcaggggatga
gatacggaacggtatacaatttccatctatcaattcgattgttcgatttcatctttgtgcaatgcaatgcaattgca
aatgcaaatgcatgatgattttccttgttgatttctccagccctgtgcttgcgcgtccaccggtgggctcgtggaca
cggtcatcgaaggcaagactggtttccacatgggccgtctcagcgtcgacgtaagcctatacatttacataacaatc
agatatgacacatcctaataccgataagtcggtacactactacacatttacatggttgctggttatatggttttttt
ggcagtgcaaggtggtggagccaagcgacgtgaagaaggtggcggccaccctgaagcgcgccatcaaggtcgtcggc
acgccggcgtacgaggagatggtcaggaactgcatgaaccaggacctctcctggaaggtataaattacgaaacaaat
ttaacccaaacatatactatatactccctccgcttctaaatattcaacgccgttgtcttttttaaatatgtttgacc
attcgtcttattaaaaaaattaaataattataaattcttttcctatcatttgattcattgttaaatatacttatatg
tatacatatagttttacatatttcataaaattttttgaacaagacgaacggtcaaacatgtgctaaaaagttaacgg
tgtcgaatattcagaaacggagggagtataaacgtcttgttcagaagttcagagattcacctgtctgatgctgatga
tgattaattgtttgcaacatggatttcaggggcctgcgaagaactgggagaatgtgctcctgggcctgggcgtcgcc
ggcagcgcgccggggatcgaaggcgacgagatcgcgccgctcgccaaggagaacgtggctgctccttgaagagcctg
agatctacatatggagtgattaattaatatagcagtatatggatgagagacgaatgaaccagtggtttgtttgttgt
agtgaatttgtagctatagccaa
SEQ ID NO.2
Wxc39cDNA-1：
accattccttcagttctttgtctatctcaagacacaaataactgcagtctctctctctctctctctctctctctctc
tctctctgcttcacttctctgcttgtgttgttctgttgttcatcaggaagaacatctgcaagttatacatatatgtt
tataattctttgtttcccctcttattcagatcgatcacatgcatctttcattgctcgtttttccttacaagtagtct
catacatgctaatttctgtaaggtgttgggctggaaattaattaattaattaattgacttgccaagatccatatata
tgtcctgatattaaatcttcgttcgttatgtttggttaggctgatcaatgttattctagagtctagagaaacacacc
caggggttttccaactagctccacaagatggtgggctagctgacctagatttgaagtctcactccttataattattt
tatattagatcattttctaatattcgtgtctttttttattctagagtctagatcttgtgttcaactctcgttaaatc
atgtctctcgccactggagaaacagatcaggagggtttattttgggtataggtcaaagctaagattgaaattcacaa
atagtaaaatcagaatccaaccaattttagtagccgagttggtcaaaggaaaatgtatatagctagatttattgttt
tggcaaaaaaaaatctgaatatgcaaaatacttgtatatctttgtattaagaagatgaaaataagtagcagaaaatt
aaaaaatggattatatttcctgggctaaaagaattgttgatttggcacaattaaattcagtgtcaaggttttgtgca
agaattcagtgtgaaggaatagattctcttcaaaacaatttaatcattcatctgatctgctcaaagctctgtgcatc
tccgggtgcaacggccaggatatttattgtgcagtaaaaaaatgtcatatcccctagccacccaagaaactgctcct
taagtccttataagcacatatggcattgtaatatatatgtttgagttttagcgacaatttttttaaaaacttttggt
cctttttatgaacgttttaagtttcactgtctttttttttcgaattttaaatgtagcttcaaattctaatccccaat
ccaaattgtaataaacttcaattctcctaattaacatcttaattcatttatttgaaaaccagttcaaattcttttag
gctcaccaaaccttaaacaattcaattcagtgcagagatcttccacagcaacagctagacaaccaccatgtcggctc
tcaccacgtcccagctcgccacctcggccaccggcttcggcatcgccgacaggtcggcgccgtcgtcgctgctccgc
cacgggttccagggcctcaagccccgcagccccgccggcggcgctcagcgtgacgaccagcgcgcgcgcgacgccca
agcagcagcggtcggtgcagcgtggcagccggaggttcccctccgtcgtcgtgtacgccaccggcgccggcatgaac
gtcgtgttcgtcggcgccgagatggccccctggagcaagaccggcggcctcggtgacgtcctcggtggcctcccccc
tgccatggctgcgaatggccacagggtcatggtgatctctcctcggtacgaccagtacaaggacgcttgggatacca
gcgttgtggctgagatcaaggttgcagacaggtacgagagggtgaggtttttccattgctacaagcgtggagtcgac
cgtgtgttcatcgaccatccgtcattcctggagaaggtttggggaaagaccggtgagaagatctacggacctgacac
tggagttgattacaaagacaaccagatgcgtttcagccttctttgccaggcagcactcgaggctcctaggatcctaa
acctcaacaacaacccatacttcaaaggaacttatggtgaggatgttgtgttcgtctgcaacgactggcacactggc
ccactggcgagctacctgaagaacaactaccagcccaatggcatctacaggaatgcaaaggttgctttctgcatcca
caacatctcctaccagggccgtttcgctttcgaggattaccctgagctgaacctctccgagaggttcaggtcatcct
tcgatttcatcgacgggtatgacacgccggtggagggcaggaagatcaactggatgaaggccggaatcctggaagcc
gacagggtgctcaccgtgagcccgtactacgccgaggagctcatctccggcatcgccaggggatgcgagctcgacaa
catcatgcggctcaccggcatcaccggcatcgtcaacggcatggacgtcagcgagtgggatcctagcaaggacaagt
acatcaccgccaagtacgacgcaaccacggcaatcgaggcgaaggcgctgaacaaggaggcgttgcaggcggaggcg
ggtcttccggtcgacaggaaaatcccactgatcgcgttcatcggcaggctggaggaacagaagggccctgacgtcat
ggccgccgccatcccggagctcatgcaggaggacgtccagatcgttcttctgggtactggaaagaagaagttcgaga
agctgctcaagagcatggaggagaagtatccgggcaaggtgagggccgtggtgaagttcaacgcgccgcttgctcat
ctcatcatggccggagccgacgtgctcgccgtccccagccgcttcgagccctgtggactcatccagctgcaggggat
gagatacggaacgccctgtgcttgcgcgtccaccggtgggctcgtggacacggtcatcgaaggcaagactggtttcc
acatgggccgtctcagcgtcgactgcaaggtggtggagccaagcgacgtgaagaaggtggcggccaccctgaagcgc
gccatcaaggtcgtcggcacgccggcgtacgaggagatggtcaggaactgcatgaaccaggacctctcctggaaggg
gcctgcgaagaactgggagaatgtgctcctgggcctgggcgtcgccggcagcgcgccggggatcgaaggcgacgaga
tcgcgccgctcgccaaggagaacgtggctgctccttga
SEQ ID NO.3
Wxc39cDNA-2：
accattccttcagttctttgtctatctcaagacacaaataactgcagtctctctctctctctctctctctctctctc
tctctctgcttcacttctctgcttgtgttgttctgttgttcatcaggaagaacatctgcaagtgcagagatcttcca
cagcaacagctagacaaccaccatgtcggctctcaccacgtcccagctcgccacctcggccaccggcttcggcatcg
ccgacaggtcggcgccgtcgtcgctgctccgccacgggttccagggcctcaagccccgcagccccgccggcggcgct
cagcgtgacgaccagcgcgcgcgcgacgcccaagcagcagcggtcggtgcagcgtggcagccggaggttcccctccg
tcgtcgtgtacgccaccggcgccggcatgaacgtcgtgttcgtcggcgccgagatggccccctggagcaagaccggc
ggcctcggtgacgtcctcggtggcctcccccctgccatggctgcgaatggccacagggtcatggtgatctctcctcg
gtacgaccagtacaaggacgcttgggataccagcgttgtggctgagatcaaggttgcagacaggtacgagagggtga
ggtttttccattgctacaagcgtggagtcgaccgtgtgttcatcgaccatccgtcattcctggagaaggtttgggga
aagaccggtgagaagatctacggacctgacactggagttgattacaaagacaaccagatgcgtttcagccttctttg
ccaggcagcactcgaggctcctaggatcctaaacctcaacaacaacccatacttcaaaggaacttatggtgaggatg
ttgtgttcgtctgcaacgactggcacactggcccactggcgagctacctgaagaacaactaccagcccaatggcatc
tacaggaatgcaaaggttgctttctgcatccacaacatctcctaccagggccgtttcgctttcgaggattaccctga
gctgaacctctccgagaggttcaggtcatccttcgatttcatcgacgggtatgacacgccggtggagggcaggaaga
tcaactggatgaaggccggaatcctggaagccgacagggtgctcaccgtgagcccgtactacgccgaggagctcatc
tccggcatcgccaggggatgcgagctcgacaacatcatgcggctcaccggcatcaccggcatcgtcaacggcatgga
cgtcagcgagtgggatcctagcaaggacaagtacatcaccgccaagtacgacgcaaccacggcaatcgaggcgaagg
cgctgaacaaggaggcgttgcaggcggaggcgggtcttccggtcgacaggaaaatcccactgatcgcgttcatcggc
aggctggaggaacagaagggccctgacgtcatggccgccgccatcccggagctcatgcaggaggacgtccagatcgt
tcttctgggtactggaaagaagaagttcgagaagctgctcaagagcatggaggagaagtatccgggcaaggtgaggg
ccgtggtgaagttcaacgcgccgcttgctcatctcatcatggccggagccgacgtgctcgccgtccccagccgcttc
gagccctgtggactcatccagctgcaggggatgagatacggaacgccctgtgcttgcgcgtccaccggtgggctcgt
ggacacggtcatcgaaggcaagactggtttccacatgggccgtctcagcgtcgactgcaaggtggtggagccaagcg
acgtgaagaaggtggcggccaccctgaagcgcgccatcaaggtcgtcggcacgccggcgtacgaggagatggtcagg
aactgcatgaaccaggacctctcctggaaggggcctgcgaagaactgggagaatgtgctcctgggcctgggcgtcgc
cggcagcgcgccggggatcgaaggcgacgagatcgcgccgctcgccaaggagaacgtggctgctccttga
SEQ ID NO.4
Wxc39The amino acid sequence of coding：
MSALTTSQLATSATGFGIADRSAPSSLLRHGFQGLKPRSPAGGAQRDDQRARDAQAAAVGAAWQPEVPL
RRRVRHRRRHERRVRRRRDGPLEQDRRPR
Sequence table
<110>Jiangsu Province Agriculture Science Institute
Yi Autonomous Prefecture of Chuxiong Academy of Agricultural Sciences
<120>A kind of neomorph and its application controlling rice low amylose content
<141> 2018-06-21
<160> 6
<170> SIPOSequenceListing 1.0
<210> 1
<211> 4874
<212> DNA
<213>Rice (Oryza sativa)
<400> 1
accattcctt cagttctttg tctatctcaa gacacaaata actgcagtct ctctctctct 60
ctctctctct ctctctctct ctgcttcact tctctgcttg tgttgttctg ttgttcatca 120
ggaagaacat ctgcaagtta tacatatatg tttataattc tttgtttccc ctcttattca 180
gatcgatcac atgcatcttt cattgctcgt ttttccttac aagtagtctc atacatgcta 240
atttctgtaa ggtgttgggc tggaaattaa ttaattaatt aattgacttg ccaagatcca 300
tatatatgtc ctgatattaa atcttcgttc gttatgtttg gttaggctga tcaatgttat 360
tctagagtct agagaaacac acccaggggt tttccaacta gctccacaag atggtgggct 420
agctgaccta gatttgaagt ctcactcctt ataattattt tatattagat cattttctaa 480
tattcgtgtc tttttttatt ctagagtcta gatcttgtgt tcaactctcg ttaaatcatg 540
tctctcgcca ctggagaaac agatcaggag ggtttatttt gggtataggt caaagctaag 600
attgaaattc acaaatagta aaatcagaat ccaaccaatt ttagtagccg agttggtcaa 660
aggaaaatgt atatagctag atttattgtt ttggcaaaaa aaaatctgaa tatgcaaaat 720
acttgtatat ctttgtatta agaagatgaa aataagtagc agaaaattaa aaaatggatt 780
atatttcctg ggctaaaaga attgttgatt tggcacaatt aaattcagtg tcaaggtttt 840
gtgcaagaat tcagtgtgaa ggaatagatt ctcttcaaaa caatttaatc attcatctga 900
tctgctcaaa gctctgtgca tctccgggtg caacggccag gatatttatt gtgcagtaaa 960
aaaatgtcat atcccctagc cacccaagaa actgctcctt aagtccttat aagcacatat 1020
ggcattgtaa tatatatgtt tgagttttag cgacaatttt tttaaaaact tttggtcctt 1080
tttatgaacg ttttaagttt cactgtcttt ttttttcgaa ttttaaatgt agcttcaaat 1140
tctaatcccc aatccaaatt gtaataaact tcaattctcc taattaacat cttaattcat 1200
ttatttgaaa accagttcaa attcttttag gctcaccaaa ccttaaacaa ttcaattcag 1260
tgcagagatc ttccacagca acagctagac aaccaccatg tcggctctca ccacgtccca 1320
gctcgccacc tcggccaccg gcttcggcat cgccgacagg tcggcgccgt cgtcgctgct 1380
ccgccacggg ttccagggcc tcaagccccg cagccccgcc ggcggcgctc agcgtgacga 1440
ccagcgcgcg cgcgacgccc aagcagcagc ggtcggtgca gcgtggcagc cggaggttcc 1500
cctccgtcgt cgtgtacgcc accggcgccg gcatgaacgt cgtgttcgtc ggcgccgaga 1560
tggccccctg gagcaagacc ggcggcctcg gtgacgtcct cggtggcctc ccccctgcca 1620
tggctgtaag cacacacaaa cttcgatcgc tcgtcgtcgc tgaccgtcgt cgtcttcaac 1680
tgttcttgat catcgcattg gatggatgtg taatgttgtg ttcttgtgtt ctttgcaggc 1740
gaatggccac agggtcatgg tgatctctcc tcggtacgac cagtacaagg acgcttggga 1800
taccagcgtt gtggctgagg taggagcata tgcgtgatca gatcatcaca agatcgatta 1860
gctttagatg atttgttaca tttcgcaaga ttttaaccca agtttttgtg gtgcaattca 1920
ttgcagatca aggttgcaga caggtacgag agggtgaggt ttttccattg ctacaagcgt 1980
ggagtcgacc gtgtgttcat cgaccatccg tcattcctgg agaaggtgga gtcatcatta 2040
gtttaccttt tttgttttta ctgaattatt aacagtgcat ttagcagttg gactgagctt 2100
agcttccact ggtgatttca ggtttgggga aagaccggtg agaagatcta cggacctgac 2160
actggagttg attacaaaga caaccagatg cgtttcagcc ttctttgcca ggtcagtgat 2220
tacttctatc tgatgatggt tggaagcatc acgagtttac catagtatgt atggattcat 2280
aactaattcg tgtattgatg ctacctgcag gcagcactcg aggctcctag gatcctaaac 2340
ctcaacaaca acccatactt caaaggaact tatggtgagt tacaattgat ctcaagatct 2400
tataactttc ttcgaaggaa tccatgatga tcagactaat tccttccggt ttgttactga 2460
caacaggtga ggatgttgtg ttcgtctgca acgactggca cactggccca ctggcgagct 2520
acctgaagaa caactaccag cccaatggca tctacaggaa tgcaaaggtc tatgcttgtt 2580
cttgccatac caactcaaat ctgcatgcac actgcattct gttcagaaac tgactgtctg 2640
aatctttttc actgcaggtt gctttctgca tccacaacat ctcctaccag ggccgtttcg 2700
ctttcgagga ttaccctgag ctgaacctct ccgagaggtt caggtcatcc ttcgatttca 2760
tcgacgggta tgagtaagat tctaagagta acttactgtc aattcgccat atatcgattc 2820
aatccaagat ccttttgagc tgacaaccct gcactactgt ccatcgttca aatccggtta 2880
aatttcaggt atgacacgcc ggtggagggc aggaagatca actggatgaa ggccggaatc 2940
ctggaagccg acagggtgct caccgtgagc ccgtactacg ccgaggagct catctccggc 3000
atcgccaggg gatgcgagct cgacaacatc atgcggctca ccggcatcac cggcatcgtc 3060
aacggcatgg acgtcagcga gtgggatcct agcaaggaca agtacatcac cgccaagtac 3120
gacgcaacca cggtaagaac gaatgcattc ttcacaagat atgcaatctg aattttcttt 3180
gaaaaagaaa ttatcatctg tcacttcttg attgattctg acaaggcaag aatgagtgac 3240
aaatttcagg caatcgaggc gaaggcgctg aacaaggagg cgttgcaggc ggaggcgggt 3300
cttccggtcg acaggaaaat cccactgatc gcgttcatcg gcaggctgga ggaacagaag 3360
ggccctgacg tcatggccgc cgccatcccg gagctcatgc aggaggacgt ccagatcgtt 3420
cttctggtat aatataatac actacaagac acacttgcac gatatgccaa aaattcagaa 3480
caaattcagt ggcaaaaaaa aaactcgaat attagggaag gacctaataa tatcaaataa 3540
ttagaagggg tgaggctttg aacccagatc gtctagtcca ccaccttgtg gagttagccg 3600
gaagacctct gagcatttct caattcagtg gcaaatgatg tgtataattt tgatccgtgt 3660
gtgtttcagg gtactggaaa gaagaagttc gagaagctgc tcaagagcat ggaggagaag 3720
tatccgggca aggtgagggc cgtggtgaag ttcaacgcgc cgcttgctca tctcatcatg 3780
gccggagccg acgtgctcgc cgtccccagc cgcttcgagc cctgtggact catccagctg 3840
caggggatga gatacggaac ggtatacaat ttccatctat caattcgatt gttcgatttc 3900
atctttgtgc aatgcaatgc aattgcaaat gcaaatgcat gatgattttc cttgttgatt 3960
tctccagccc tgtgcttgcg cgtccaccgg tgggctcgtg gacacggtca tcgaaggcaa 4020
gactggtttc cacatgggcc gtctcagcgt cgacgtaagc ctatacattt acataacaat 4080
cagatatgac acatcctaat accgataagt cggtacacta ctacacattt acatggttgc 4140
tggttatatg gtttttttgg cagtgcaagg tggtggagcc aagcgacgtg aagaaggtgg 4200
cggccaccct gaagcgcgcc atcaaggtcg tcggcacgcc ggcgtacgag gagatggtca 4260
ggaactgcat gaaccaggac ctctcctgga aggtataaat tacgaaacaa atttaaccca 4320
aacatatact atatactccc tccgcttcta aatattcaac gccgttgtct tttttaaata 4380
tgtttgacca ttcgtcttat taaaaaaatt aaataattat aaattctttt cctatcattt 4440
gattcattgt taaatatact tatatgtata catatagttt tacatatttc ataaaatttt 4500
ttgaacaaga cgaacggtca aacatgtgct aaaaagttaa cggtgtcgaa tattcagaaa 4560
cggagggagt ataaacgtct tgttcagaag ttcagagatt cacctgtctg atgctgatga 4620
tgattaattg tttgcaacat ggatttcagg ggcctgcgaa gaactgggag aatgtgctcc 4680
tgggcctggg cgtcgccggc agcgcgccgg ggatcgaagg cgacgagatc gcgccgctcg 4740
ccaaggagaa cgtggctgct ccttgaagag cctgagatct acatatggag tgattaatta 4800
atatagcagt atatggatga gagacgaatg aaccagtggt ttgtttgttg tagtgaattt 4860
gtagctatag ccaa 4874
<210> 3
<211> 3118
<212> DNA
<213>Rice (Oryza sativa)
<400> 3
accattcctt cagttctttg tctatctcaa gacacaaata actgcagtct ctctctctct 60
ctctctctct ctctctctct ctctgcttca cttctctgct tgtgttgttc tgttgttcat 120
caggaagaac atctgcaagt tatacatata tgtttataat tctttgtttc ccctcttatt 180
cagatcgatc acatgcatct ttcattgctc gtttttcctt acaagtagtc tcatacatgc 240
taatttctgt aaggtgttgg gctggaaatt aattaattaa ttaattgact tgccaagatc 300
catatatatg tcctgatatt aaatcttcgt tcgttatgtt tggttaggct gatcaatgtt 360
attctagagt ctagagaaac acacccaggg gttttccaac tagctccaca agatggtggg 420
ctagctgacc tagatttgaa gtctcactcc ttataattat tttatattag atcattttct 480
aatattcgtg tcttttttta ttctagagtc tagatcttgt gttcaactct cgttaaatca 540
tgtctctcgc cactggagaa acagatcagg agggtttatt ttgggtatag gtcaaagcta 600
agattgaaat tcacaaatag taaaatcaga atccaaccaa ttttagtagc cgagttggtc 660
aaaggaaaat gtatatagct agatttattg ttttggcaaa aaaaaatctg aatatgcaaa 720
atacttgtat atctttgtat taagaagatg aaaataagta gcagaaaatt aaaaaatgga 780
ttatatttcc tgggctaaaa gaattgttga tttggcacaa ttaaattcag tgtcaaggtt 840
ttgtgcaaga attcagtgtg aaggaataga ttctcttcaa aacaatttaa tcattcatct 900
gatctgctca aagctctgtg catctccggg tgcaacggcc aggatattta ttgtgcagta 960
aaaaaatgtc atatccccta gccacccaag aaactgctcc ttaagtcctt ataagcacat 1020
atggcattgt aatatatatg tttgagtttt agcgacaatt tttttaaaaa cttttggtcc 1080
tttttatgaa cgttttaagt ttcactgtct ttttttttcg aattttaaat gtagcttcaa 1140
attctaatcc ccaatccaaa ttgtaataaa cttcaattct cctaattaac atcttaattc 1200
atttatttga aaaccagttc aaattctttt aggctcacca aaccttaaac aattcaattc 1260
agtgcagaga tcttccacag caacagctag acaaccacca tgtcggctct caccacgtcc 1320
cagctcgcca cctcggccac cggcttcggc atcgccgaca ggtcggcgcc gtcgtcgctg 1380
ctccgccacg ggttccaggg cctcaagccc cgcagccccg ccggcggcgc tcagcgtgac 1440
gaccagcgcg cgcgcgacgc ccaagcagca gcggtcggtg cagcgtggca gccggaggtt 1500
cccctccgtc gtcgtgtacg ccaccggcgc cggcatgaac gtcgtgttcg tcggcgccga 1560
gatggccccc tggagcaaga ccggcggcct cggtgacgtc ctcggtggcc tcccccctgc 1620
catggctgcg aatggccaca gggtcatggt gatctctcct cggtacgacc agtacaagga 1680
cgcttgggat accagcgttg tggctgagat caaggttgca gacaggtacg agagggtgag 1740
gtttttccat tgctacaagc gtggagtcga ccgtgtgttc atcgaccatc cgtcattcct 1800
ggagaaggtt tggggaaaga ccggtgagaa gatctacgga cctgacactg gagttgatta 1860
caaagacaac cagatgcgtt tcagccttct ttgccaggca gcactcgagg ctcctaggat 1920
cctaaacctc aacaacaacc catacttcaa aggaacttat ggtgaggatg ttgtgttcgt 1980
ctgcaacgac tggcacactg gcccactggc gagctacctg aagaacaact accagcccaa 2040
tggcatctac aggaatgcaa aggttgcttt ctgcatccac aacatctcct accagggccg 2100
tttcgctttc gaggattacc ctgagctgaa cctctccgag aggttcaggt catccttcga 2160
tttcatcgac gggtatgaca cgccggtgga gggcaggaag atcaactgga tgaaggccgg 2220
aatcctggaa gccgacaggg tgctcaccgt gagcccgtac tacgccgagg agctcatctc 2280
cggcatcgcc aggggatgcg agctcgacaa catcatgcgg ctcaccggca tcaccggcat 2340
cgtcaacggc atggacgtca gcgagtggga tcctagcaag gacaagtaca tcaccgccaa 2400
gtacgacgca accacggcaa tcgaggcgaa ggcgctgaac aaggaggcgt tgcaggcgga 2460
ggcgggtctt ccggtcgaca ggaaaatccc actgatcgcg ttcatcggca ggctggagga 2520
acagaagggc cctgacgtca tggccgccgc catcccggag ctcatgcagg aggacgtcca 2580
gatcgttctt ctgggtactg gaaagaagaa gttcgagaag ctgctcaaga gcatggagga 2640
gaagtatccg ggcaaggtga gggccgtggt gaagttcaac gcgccgcttg ctcatctcat 2700
catggccgga gccgacgtgc tcgccgtccc cagccgcttc gagccctgtg gactcatcca 2760
gctgcagggg atgagatacg gaacgccctg tgcttgcgcg tccaccggtg ggctcgtgga 2820
cacggtcatc gaaggcaaga ctggtttcca catgggccgt ctcagcgtcg actgcaaggt 2880
ggtggagcca agcgacgtga agaaggtggc ggccaccctg aagcgcgcca tcaaggtcgt 2940
cggcacgccg gcgtacgagg agatggtcag gaactgcatg aaccaggacc tctcctggaa 3000
ggggcctgcg aagaactggg agaatgtgct cctgggcctg ggcgtcgccg gcagcgcgcc 3060
ggggatcgaa ggcgacgaga tcgcgccgct cgccaaggag aacgtggctg ctccttga 3118
<210> 4
<211> 1995
<212> DNA
<213>Rice (Oryza sativa)
<400> 4
accattcctt cagttctttg tctatctcaa gacacaaata actgcagtct ctctctctct 60
ctctctctct ctctctctct ctctgcttca cttctctgct tgtgttgttc tgttgttcat 120
caggaagaac atctgcaagt gcagagatct tccacagcaa cagctagaca accaccatgt 180
cggctctcac cacgtcccag ctcgccacct cggccaccgg cttcggcatc gccgacaggt 240
cggcgccgtc gtcgctgctc cgccacgggt tccagggcct caagccccgc agccccgccg 300
gcggcgctca gcgtgacgac cagcgcgcgc gcgacgccca agcagcagcg gtcggtgcag 360
cgtggcagcc ggaggttccc ctccgtcgtc gtgtacgcca ccggcgccgg catgaacgtc 420
gtgttcgtcg gcgccgagat ggccccctgg agcaagaccg gcggcctcgg tgacgtcctc 480
ggtggcctcc cccctgccat ggctgcgaat ggccacaggg tcatggtgat ctctcctcgg 540
tacgaccagt acaaggacgc ttgggatacc agcgttgtgg ctgagatcaa ggttgcagac 600
aggtacgaga gggtgaggtt tttccattgc tacaagcgtg gagtcgaccg tgtgttcatc 660
gaccatccgt cattcctgga gaaggtttgg ggaaagaccg gtgagaagat ctacggacct 720
gacactggag ttgattacaa agacaaccag atgcgtttca gccttctttg ccaggcagca 780
ctcgaggctc ctaggatcct aaacctcaac aacaacccat acttcaaagg aacttatggt 840
gaggatgttg tgttcgtctg caacgactgg cacactggcc cactggcgag ctacctgaag 900
aacaactacc agcccaatgg catctacagg aatgcaaagg ttgctttctg catccacaac 960
atctcctacc agggccgttt cgctttcgag gattaccctg agctgaacct ctccgagagg 1020
ttcaggtcat ccttcgattt catcgacggg tatgacacgc cggtggaggg caggaagatc 1080
aactggatga aggccggaat cctggaagcc gacagggtgc tcaccgtgag cccgtactac 1140
gccgaggagc tcatctccgg catcgccagg ggatgcgagc tcgacaacat catgcggctc 1200
accggcatca ccggcatcgt caacggcatg gacgtcagcg agtgggatcc tagcaaggac 1260
aagtacatca ccgccaagta cgacgcaacc acggcaatcg aggcgaaggc gctgaacaag 1320
gaggcgttgc aggcggaggc gggtcttccg gtcgacagga aaatcccact gatcgcgttc 1380
atcggcaggc tggaggaaca gaagggccct gacgtcatgg ccgccgccat cccggagctc 1440
atgcaggagg acgtccagat cgttcttctg ggtactggaa agaagaagtt cgagaagctg 1500
ctcaagagca tggaggagaa gtatccgggc aaggtgaggg ccgtggtgaa gttcaacgcg 1560
ccgcttgctc atctcatcat ggccggagcc gacgtgctcg ccgtccccag ccgcttcgag 1620
ccctgtggac tcatccagct gcaggggatg agatacggaa cgccctgtgc ttgcgcgtcc 1680
accggtgggc tcgtggacac ggtcatcgaa ggcaagactg gtttccacat gggccgtctc 1740
agcgtcgact gcaaggtggt ggagccaagc gacgtgaaga aggtggcggc caccctgaag 1800
cgcgccatca aggtcgtcgg cacgccggcg tacgaggaga tggtcaggaa ctgcatgaac 1860
caggacctct cctggaaggg gcctgcgaag aactgggaga atgtgctcct gggcctgggc 1920
gtcgccggca gcgcgccggg gatcgaaggc gacgagatcg cgccgctcgc caaggagaac 1980
gtggctgctc cttga 1995
<210> 5
<211> 98
<212> PRT
<213>Rice (Oryza sativa)
<400> 5
Met Ser Ala Leu Thr Thr Ser Gln Leu Ala Thr Ser Ala Thr Gly Phe
1 5 10 15
Gly Ile Ala Asp Arg Ser Ala Pro Ser Ser Leu Leu Arg His Gly Phe
20 25 30
Gln Gly Leu Lys Pro Arg Ser Pro Ala Gly Gly Ala Gln Arg Asp Asp
35 40 45
Gln Arg Ala Arg Asp Ala Gln Ala Ala Ala Val Gly Ala Ala Trp Gln
50 55 60
Pro Glu Val Pro Leu Arg Arg Arg Val Arg His Arg Arg Arg His Glu
65 70 75 80
Arg Arg Val Arg Arg Arg Arg Asp Gly Pro Leu Glu Gln Asp Arg Arg
85 90 95
Pro Arg
<210> 5
<211> 21
<212> DNA
<213>Artificial sequence (Artificial Sequence)
<400> 5
cggtgcccaa cagaaaccac a 21
<210> 6
<211> 20
<212> DNA
<213>Artificial sequence (Artificial Sequence)
<400> 6
cacccagaag agtacaacat 20
Claims (5)
1. a kind of content of amylose in rice neomorph, gene Wxc39Gene accession number is the rice Waxy of EU770319
129th to 139 nucleotide deletion of gene coding region exon 2；The Wxc39Its nucleotide sequence of gene such as SEQ
Shown in ID No.1.
2. gene according to claim 1, it is characterised in that：Gene Wxc39Containing there are two transcription products, respectively such as SEQ
Shown in ID No.2 and SEQ ID No.3.
3. the albumen of coded by said gene of any of claims 1 or 2, it is characterised in that：Its amino acid sequence such as SEQ ID No.4
It is shown.
4. application of the gene of any of claims 1 or 2 in paddy rice cross breeding breeding and breed improvement.
5. application according to claim 4, which is characterized in that refer to answering in terms of controlling rice low amylose content
With.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810743434.9A CN108841838B (en) | 2018-07-09 | 2018-07-09 | Novel allele for controlling low amylose content of rice and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810743434.9A CN108841838B (en) | 2018-07-09 | 2018-07-09 | Novel allele for controlling low amylose content of rice and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108841838A true CN108841838A (en) | 2018-11-20 |
| CN108841838B CN108841838B (en) | 2021-08-17 |
Family
ID=64195824
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810743434.9A Active CN108841838B (en) | 2018-07-09 | 2018-07-09 | Novel allele for controlling low amylose content of rice and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108841838B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111197034A (en) * | 2020-01-08 | 2020-05-26 | 江苏省农业科学院 | Wx mutant protein based on gene editing technology and application of gene thereof in plant breeding |
| CN111424036A (en) * | 2020-03-16 | 2020-07-17 | 华中农业大学 | New rice Wx allele and application thereof in breeding |
| CN114438101A (en) * | 2022-03-10 | 2022-05-06 | 江苏省农业科学院 | Allele with transparent rice appearance and low amylose content and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101792805A (en) * | 2010-03-18 | 2010-08-04 | 江苏省农业科学院 | Molecular markers of paddy endosperm low amylose content gene Wx-mq |
| CN105087573A (en) * | 2015-09-14 | 2015-11-25 | 扬州大学 | Method for identifying rice Wx-mw gene and application of rice Wx-mw gene in high-quality rice breeding |
-
2018
- 2018-07-09 CN CN201810743434.9A patent/CN108841838B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101792805A (en) * | 2010-03-18 | 2010-08-04 | 江苏省农业科学院 | Molecular markers of paddy endosperm low amylose content gene Wx-mq |
| CN105087573A (en) * | 2015-09-14 | 2015-11-25 | 扬州大学 | Method for identifying rice Wx-mw gene and application of rice Wx-mw gene in high-quality rice breeding |
Non-Patent Citations (3)
| Title |
|---|
| SAMART WANCHANA等: "Duplicated coding sequence in the waxy allele of tropical glutinous rice (Oryza sativa L.)", 《PLANT SCIENCE》 * |
| 熊华等: "《现代糯稻生产技术》", 31 March 2017, 湖北科学技术出版社 * |
| 程式华等: "《现代中国水稻》", 31 January 2007, 金盾出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111197034A (en) * | 2020-01-08 | 2020-05-26 | 江苏省农业科学院 | Wx mutant protein based on gene editing technology and application of gene thereof in plant breeding |
| CN111424036A (en) * | 2020-03-16 | 2020-07-17 | 华中农业大学 | New rice Wx allele and application thereof in breeding |
| CN111424036B (en) * | 2020-03-16 | 2021-11-02 | 华中农业大学 | New rice Wx allele and application thereof in breeding |
| CN114438101A (en) * | 2022-03-10 | 2022-05-06 | 江苏省农业科学院 | Allele with transparent rice appearance and low amylose content and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108841838B (en) | 2021-08-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wang et al. | Fine mapping and candidate gene analysis of purple pericarp gene Pb in rice (Oryza sativa L.) | |
| CN102057045B (en) | transgenic sugar beet plants | |
| US10611808B2 (en) | Isolated polypeptides and polynucleotides encoding same for generating plants with increased cuticlar water permeability | |
| EP3004356B1 (en) | Wheat stem rust resistance gene | |
| CN108841838A (en) | A kind of neomorph and its application controlling rice low amylose content | |
| CN102816243A (en) | Application of rice transcription factor Os06g08400 genes | |
| CN103865937B (en) | Cytoplasmic male sterility restorer gene in rice and application thereof | |
| CN104946660B (en) | DNA subsequence of maize pollen postmeiotic development control gene Ms30 and protein encoded by same | |
| CN112080515B (en) | UP gene and application thereof in plant improvement | |
| US11732014B2 (en) | Modulation of Solanaceae fruit ripening | |
| CN107949273A (en) | Fruit has the tomato plants of yellow and red section | |
| CN108531645B (en) | Functional marker of low amylose content gene wx-C39 and application thereof | |
| CN114381543A (en) | Molecular marker for detecting SNP locus of cucumber Cspsy1 gene and application thereof | |
| CN108441571A (en) | Application of the corn molecular labeling in identifying and regulating and controlling corn rough dwarf resistance trait | |
| KR101805164B1 (en) | Method for determining orange color of vegetable fruit | |
| CN106793762A (en) | Tomato plants with pink gloss fruit | |
| CN101845085B (en) | Wild soybean AP1 type protein and coding gene and application thereof | |
| CN111235168B (en) | Receptor kinase SlSRK2B participating in lycopene synthesis and application thereof | |
| CN114574622A (en) | SSR molecular marker combination for identifying teal new strain No.1, fingerprint spectrum and application of SSR molecular marker combination | |
| CN114438101A (en) | Allele with transparent rice appearance and low amylose content and application thereof | |
| CN108977414B (en) | Artificially synthesized mutant of beta-carotene ketolase and coding sequence and application thereof | |
| CN114134157A (en) | Application of IbSAP15 gene in regulation and control of leaf type and flower type of sweet potato | |
| CN104878006B (en) | Aleurone is specifically expressed, adjusts gene and its application of size of plant seed and starch quality | |
| WO2005072515A1 (en) | High lycopene tomato varieties and use thereof | |
| CN113151295A (en) | Rice temperature-sensitive male sterile gene OsFMS1 and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |