CN108841752A - The application of bacillus megaterium BM22 and its gemma liquid preparation in prevention and treatment cyclamen black root - Google Patents
The application of bacillus megaterium BM22 and its gemma liquid preparation in prevention and treatment cyclamen black root Download PDFInfo
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Abstract
The invention discloses the application of bacillus megaterium BM22 and its gemma liquid preparation in prevention and treatment cyclamen black root, the bacillus megaterium BM22 has carried out preservation, depositary institution：China General Microbiological culture presevation administrative center, deposit number are CGMCC No.15070.The bacillus megaterium BM22 gemma liquid preparation is strain by bacillus megaterium BM22, obtains liquid preparation through seed culture, fermented and cultured.The brood-gemma that bacillus megaterium BM2 of the present invention has resistance strong is suitable for merchandized handling, and has disease prevention growth-promoting dual function.
Description
Technical field
The invention belongs to microorganisms technical fields, and it is celestial in prevention and treatment to be related to bacillus megaterium BM22 and its gemma liquid preparation
Visitor carrys out the application in black root.
Background technique
Cyclamen (Cyclamen persicum Mill), alias radish Malus spectabilis, cyclamen, rabbit flower, product hat, bonfire
Flower turns over valve lotus, is Primulaceae, Cyclamen herbaceos perennial, blade at the top of stem tuber by bearing, heart-shaped, oval or kidney
Shape, blade have serration, and blade face green has white or grey milky spot, blade back green or kermesinus, and petiole is longer, bronzing.
The happiness of cyclamen property is warm, is afraid of sweltering heat, grows under nice and cool environment and in rich humus-containing fertile sandy loam best.It is more resistance to
Cold, being resistant to 0 DEG C of low temperature will not endure cold.Autumn to the 2nd year spring is its season of growth, summer semidormancy, the life that winter is suitable for
For long temperature between 12~16 DEG C, when Accelerate bloom, is not to be exceeded 18~22 DEG C, and 0 DEG C or more plant will enter suspend mode, 35 DEG C with
Upper plant is perishable, dead, and winter can be low temperature resistant, but 5 DEG C or less then slow growths, and pattern is dim, blooms few.Winter supplement two
Carbon oxide gas can promote growth and bloom.In growth period, it is desirable that with the humid air and sunshine abundance environment.
Cyclamen black root is also known as seedling maize ear rot, is a kind of serious plant disease on cyclamen, causes root system to rot, whole strain is dead
It dies.Germ invades from root, and diseased plant branch root rot disappears, and main root rots in dark brown, aerial part blade pumping expansion evening, exhibition
It is withered, sagging to open leaf.Diseased plant is easily concurrent with soft rot, and stem tuber softening is caused to be rotted.It is mould by the root string strain of Deuteromycotina
(Thielaviopsis basicola (Berk.etBr.) Ferr.) causes, and germ is overwintering in the soil with invalid body, and next year exists
Field carries out First aggression.111 conidiums of soil band and chlamydospore every gram of field.Germ is passed by water flow or irrigation water
Broadcast sprawling, soil moisture, continuous cropping, plant morbidity is heavier.The disease mainly chemical prevention at present, but environmentally friendly, people and animals
Safety, sustainable use biological control be not directed to.
Summary of the invention
The technical problem to be solved by the present invention is to, in the defects of prevention and treatment cyclamen black root, provide for the prior art
The application of bacillus megaterium BM22 and its liquid preparation in prevention and treatment cyclamen black root.
The present invention is realized especially by following technical scheme：
Bacillus megaterium (Bacillus megaterium) BM22 bacterial strain of the invention was used on June 15th, 2017
Dilution plating procedure is located away from Sichuan Dayi and looks up to heaven nest medicine field health Eucommia ulmoides Oliv. leaves, is preserved on December 15th, 2017
China Committee for Culture Collection of Microorganisms's common micro-organisms center of Institute of Microorganism, Academia Sinica (CGMCC), address
Positioned at Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, deposit number is CGMCC No.15070.
The single colonie that bacillus megaterium BM22 of the present invention has cultivated 2d on NA plating medium is creamy white, round,
Rough surface, edge waviness shape is opaque, inviscid.
The 16S rDNA sequence of bacillus megaterium BM22 of the present invention is as shown in SEQ ID NO.1.
The present invention is bacterium by bacillus megaterium BM22 using bacillus megaterium BM22 as the liquid preparation of active constituent
Kind, liquid preparation is obtained through seed culture, fermented and cultured.
It is of the present invention using bacillus megaterium BM22 as number containing living spores in the liquid preparation of strain not less than 1 ×
109cfu/g。
It is of the present invention to be prepared by the following method by the gemma liquid preparation of strain of bacillus megaterium BM22：
1) primary inclined plane seed：Beef extract-peptone slant medium is made, after being inoculated with bacillus megaterium BM22, culture
First order seed is made；
2) secondary liquid seed：Nutrition meat culture solution is contained in triangular flask under the control of logical oxygen, in nothing after high pressure sterilization
Bacterium state is inoculated with the inclined-plane bacillus megaterium BM22 seed, and bacillus megaterium BM22 liquid seeds are made in shaken cultivation；
3) fermentation culture is contained in triangular flask under the control of logical oxygen after high pressure sterilization, is inoculated with huge bud in germ-free condition
Gemma liquid preparation is made after inoculum concentration is the 15%, shaken cultivation 4 days of total liquid volume in spore bacillus liquid seeds.
The beef extract-peptone slant medium is：Beef extract 5g, peptone 10g, NaCl 3.5g, agar 18g,
Water 1000mL.
The nutrition meat culture solution is：Beef extract 7g, peptone 10g, NaCl 5g, cyclamen root skin maceration extract
1000mL。
The fermentation culture is：Maize flour 0.5-0.6%, cornstarch 0.08~0.2%, wheat bran 0.9~
1.2%, glucose 0.18~0.54%, soybean cake powder 0.05~0.15%, soy meal 0.2~0.5%, KH2PO40.005~
0.006%, (NH4)2SO40.006~0.008%, MnSO40.004~0.005%, CaCO30.0005~0.002%,
MgSO4·7H2The cyclamen root maceration extract of O 0.005~0.006% and surplus, pH 7.0~7.5.
Application of the bacillus megaterium BM22 of the present invention in prevention and treatment cyclamen black root.
Application of the bacillus megaterium BM22 gemma liquid preparation of the present invention in prevention and treatment cyclamen black root, tool
Body is to carry out the application of spraying or pouring root in seedling stage or in growing process.
For the liquid preparation in the application for the treatment of cyclamen black root, liquid preparation dilution is by liquid preparation system
It is 1 × 10 at concentration9Then cfu/mL dilutes application again.
Beneficial effects of the present invention are：
Compared with the technical solution of existing cyclamen black root, biocontrol microorganisms bacillus megaterium that the present invention screens
(Bacillus megaterium) BM22 bacterial strain, with the strong gemma of resistance, commodity shelf-life is long, to environment fitness
By force, commercial preparation can be produced into；The nuisanceless prevention and control of bacillus megaterium (Bacillus megaterium) BM22 bacterial strain are celestial
Visitor carrys out black root, and promotes Growth of Cyclamen.
Detailed description of the invention
Fig. 1 is bacillus megaterium BM22rDNA-ITS sequence pcr amplification product electrophoretogram of the present invention；
Fig. 2 is the phylogenetic tree of the bacterial strain BM22 based on 16S rDNA sequence construct.
Specific embodiment
Technical solution of the present invention is further described below with reference to embodiment, it is as described below, it is only to of the invention
Preferred embodiment not limits the present invention, any person skilled in the art possibly also with
The technology contents of the disclosure above are changed or are modified as the equivalent embodiment changed on an equal basis.It is all without departing from the present invention program
Hold, according to the technical essence of the invention any simple modification, equivalent change and modification made to the above embodiment, all falls within this
In the protection scope of invention.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
The separation and identification of 1 bacillus megaterium BM22 of embodiment
Separation, purifying and the preservation of 1.1 endogenetic bacterias
Healthy Eucommia ulmoides Oliv. leaves are taken in Sichuan Dayi nest medicine field Cortex Eucommiae black spot generating region of looking up to heaven.The sample sterile water adopted back
It rinses well, weighs 1.0g tissue, 1-2min is impregnated in 70% alcohol, then sterilize 3- with 1-3% liquor natrii hypochloritis
5min, sterile water washing is several after, absorption 100 μ L of last time cleaning solution smearing NA plate (beef extract 3g, peptone 10g,
Sodium chloride 5g, agar powder 15-20g, distilled water 1000mL, pH 7.0 mix 121 DEG C of high pressure sterilization 30min after packing), 27 DEG C
Dark culturing for 24 hours, for the control of surface sterilization, checks whether surface sterilization is thorough.
The bark tissue of surface sterilization is shredded, sterile mortar is put into, quartz sand and the 10mL that sterilizing is added are sterile
Water is fully ground, and stands 30min, dilution 10-1, 10-2, 10-3, 10-4, 10-5Totally 5 gradients draw 100 μ L 10 respectively-3,
10-4, 10-5This 3 gradient lapping liquids are respectively coated NA plate (beef extract 3g, peptone 10g, sodium chloride 5g, agar powder 15-
20g, distilled water 1000mL, pH 7.0 mix 121 DEG C of high pressure sterilization 30min after packing), 3 repetitions of each processing, 27 DEG C of trainings
48h is supported, the apparent single colonie of picking colony morphological differences enters primary dcreening operation.
According to the size of bacterium colony, color, whether protrusion, whether edge feature, surface are smooth, the difference of transparency etc.
Different, for picking single colonie in the flat lining out purifying of NA, the inclined-plane NA of transferring after purification is spare in 4 DEG C of preservations.It obtains altogether raw thin in 36
Bacterium.
Plate antagonism of 1.2 endogenetic bacterias to cyclamen black root germ (Thielaviopsis basicola)
Tablet face-off method：In the cyclamen black root germ (Thielaviopsis basicola) of culture dish center inoculation 7d
Bacteria cake (diameter 6mm) picks the endophyte of culture 2d, in the symmetrical two sides of germ at the germ cake about 3cm with oese respectively
Each standardized filament, 28 DEG C of cultures, to be inoculated with the plate of germ only as control, the bacterium colony of measurement germ is raw when covering with ware wait compare
Long diameter, every processing are repeated 3 times.Bacterium colony growth inhibition ratio is calculated as follows：
Bacterium colony growth inhibition ratio=(the control net growth diameter of bacterium colony-net growth diameter of processing bacterium colony)/control bacterium colony is given birth to only
Long diameter × 100%.
Table 1 is that have preferable antagonism to cyclamen black root germ (Thielaviopsis basicola) in 32 plants of endogenetic bacterias
The bacterial strain of effect, wherein BM22 inhibiting rate reaches 100%.
Antagonism (7d) of 1 endogenetic bacteria of table to cyclamen black root germ
The Identification of Species of raw Antagonistic Fungi in 1.3
It is huge in conjunction with physiological and biochemical index (table 2) and molecular biology identification endophyte BM22 through morphological observation
Bacillus.
2 bacterial strain BM22 physiological and biochemical index of table
The single colonie milky of the BM22 bacterial strain of 2d has been cultivated on NA plating medium, round, rough surface, edge wave
Line shape, it is opaque, it is inviscid.
Molecular biology identification (16S rDNA)：By extracting, DNA of bacteria carries out PCR amplification and electrophoresis, recovery product are sent
It is sequenced to biotech company；To be sequenced column in GenBank database it has been reported that sequence carry out homology BLAST point
Analysis, and multiple sequence comparison is carried out with Clustalx (1.83) software, then sent out with the adjacent method building system in Mega4.0 software
Tree is educated, determines status of the BM22 bacterial strain in microflora auxology.It is 949bp's that molecular biology identification, which obtains length,
The 16S rDNA sequence of BM22 bacterial strain is submitted GenBank database and carries out BLAST analysis, chosen wherein by DNA fragmentation (Fig. 1)
The higher bacterial 16 S rDNA sequence of homology therewith, and multiple matching arrangement analysis is carried out with Clustalx software, use Mega
It analyzes software building phylogenetic tree (Fig. 2).As can be seen that BM22 bacterial strain and JX286698 are with 99% 16SrRNA gene core
Nucleotide sequence similitude and higher value support of bootstrapping gather for 1 branch, and apart from each other with other bacillus, show BM22
It is nearest with the affiliation of Bacillus megaterium.
Based on features above, above-mentioned its classification naming of BM22 bacterial strain is bacillus megaterium (Bacillus
Megaterium) BM22 is stored in Institute of Microorganism, Academia Sinica's Chinese microorganism strain on December 15th, 2017
Preservation administration committee common micro-organisms center, deposit number are CGMCC No.15070.
The preparation of 2 bacillus megaterium BM22 gemma liquid agent of embodiment
The preparation of 1.1 gemma liquid agents
Liquid preparation is made by seed culture, fermented and cultured in the bacillus megaterium BM22 obtained according to embodiment 1,
Especially by following methods：
Conventional method makes beef extract-peptone slant medium (beef extract 5g, peptone 10g, NaCl 3.5g, agar
18g, water 1000mL), after being inoculated with bacillus megaterium, first order seed is made in culture.
Nutrition meat culture solution (beef extract 7g, peptone 10g, NaCl 5g, Cortex Magnoliae Officinalis root skin maceration extract 1000mL) is through high pressure
After sterilizing, under the control of logical oxygen, it is contained in the bottling of 300mL triangular flask ratio in 100mL liquid, is inoculated with huge gemma in germ-free condition
Bacillus megaterium liquid seeds are made in bacillus inclined-plane seed, 2 inclined-plane seeds of every bottle of inoculation, shaken cultivation.Wherein by 10g
Cyclamen root system boils 10 minutes in 1000mL water, filtering, and obtained filtrate is cyclamen root maceration extract.
Gemma liquid fermentation：Maize flour 0.5-0.6%, cornstarch 0.08~0.2%, wheat bran 0.9~1.2%, grape
Sugar 0.18~0.54%, soybean cake powder 0.05~0.15%, soy meal 0.2~0.5%, KH2PO40.005~0.006%,
(NH4)2SO40.006~0.008%, MnSO40.004~0.005%, CaCO30.0005~0.002%, MgSO4·7H2O
0.005~0.006% and surplus cyclamen root maceration extract, pH 7.0~7.5.Culture solution is after high pressure sterilization, in logical oxygen
It is contained in triangular flask under control, is inoculated with bacillus megaterium liquid seeds in germ-free condition, inoculum concentration is the 15% of total liquid volume,
After shaken cultivation 4 days, gemma liquid agent is made, number containing living spores is not less than 1 × 109cfu/g.The microbial inoculum can dilute when in use
Liquid is to cyclamen nursery stock pouring root.
Preparation saves：Bottled preparation is stored at room temperature 1 year or low temperature (4 DEG C) does not influence biological and ecological methods to prevent plant disease, pests, and erosion and growth-promoting effect in 2 years.
3 pouring root of embodiment applies disease prevention growth-promoting experiment
Different onset degree is divided to be diluted to various concentration pouring root soil 100mL/ with BM22 gemma liquid preparation in spring
Strain is each primary, statistical result after 30 days.Preventive effect and growth-promoting are shown in (table 3-6).It is slight to occur, with 1000 times of liquid for economic concentration, in
Degree occurs with 800 times of liquid as economic concentration, and severe occurs with 50 times to stoste as economic concentration.
Table 3BM22 gemma liquid preparation biological and ecological methods to prevent plant disease, pests, and erosion cyclamen black root
4 BM22 bacterial strain of table promotes Growth of Cyclamen (1 year raw seedling, slight generating region)
5 BM22 bacterial strain of table promotes Growth of Cyclamen (1 year raw seedling, moderate generating region)
6 BM22 bacterial strain of table promotes Growth of Cyclamen (1 year raw seedling, severe generating region)
In conclusion biocontrol microorganisms commodity shelf-life is long, it is that biological agent is commercialized and successfully gives birth to by force to environment fitness
Anti- premise and guarantee.The Bacillus megateriumBM22 bacterial strain for the control cyclamen black root that the present invention filters out, tool
There is the brood-gemma that resistance is strong, Storage is good, resistant to high temperatures, low temperature, resistance to drying, is suitable for merchandized handling, and application once may be used
It is permanently effective, can continue disease-control, cost is greatly reduced.The Bacillus megateriumBM22 bacterial strain pair that the present invention filters out
Cyclamen has disease prevention growth-promoting dual function.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Sequence table
<110>Sichuan Agricultural University
<120>The application of bacillus megaterium BM22 and its gemma liquid preparation in prevention and treatment cyclamen black root
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 949
<212> DNA
<213>Bacillus megaterium (Bacillus megaterium)
<400> 1
caggctgcgg ggctatacat gcagtcgagc gaactgatta gaagcttgct tctatgacgt 60
tagcggcgga cgggtgagta acacgtgggc aacctgcctg taagactggg ataacttcgg 120
gaaaccgaag ctaataccgg ataggatctt ctccttcatg ggagatgatt gaaagatggt 180
ttcggctatc acttacagat gggcccgcgg tgcattagct agttggtgag gtaacggctc 240
accaaggcaa cgatgcatag ccgacctgag agggtgatcg gccacactgg gactgagaca 300
cggcccagac tcctacggga ggcagcagta gggaatcttc cgcaatggac gaaagtctga 360
cggagcaacg ccgcgtgagt gatgaaggct ttcgggtcgt aaaactctgt tgttagggaa 420
gaacaagtac gagagtaact gctcgtacct tgacggtacc taaccagaaa gccacggcta 480
actacgtgcc agcagccgcg gtaatacgta ggtggcaagc gttatccgga attattgggc 540
gtaaagcgcg cgcaggcggt ttcttaagtc tgatgtgaaa gcccacggct caaccgtgga 600
gggtcattgg aaactgggga acttgagtgc agaagagaaa agcggaattc cacgtgtagc 660
ggtgaaatgc gtagagatgt ggaggaacac cagtggcgaa ggcggctttt tggtctgtaa 720
ctgacgctga ggcgcgaaag cgtggggagc aaacaggatt agataccctg gtagtccacg 780
ccgtaaacga tgagtgctaa gtgttagagg gtttccgccc tttagtgctg cagctaacgc 840
attaagcact ccgcctgggg agtacggtcg caagactgaa actcaaagga attgacgggg 900
gcccgcacaa gcggtggagc atgtggttta attcgaagca acgcgaaga 949
Claims (9)
1. application of the bacillus megaterium BM22 in prevention and treatment cyclamen black root.
2. application according to claim 1, which is characterized in that the bacillus megaterium BM22 has carried out preservation, protects
Hide unit：China General Microbiological culture presevation administrative center, deposit number are CGMCC No.15070.
3. application according to claim 1, which is characterized in that the 16S rDNA sequence of the bacillus megaterium BM22
As shown in SEQ ID NO.1.
4. application of the bacillus megaterium BM22 gemma liquid preparation described in claim 1 in prevention and treatment cyclamen black root.
5. application according to claim 4, which is characterized in that number containing living spores is not less than in the gemma liquid preparation
1×109cfu/g。
6. application according to claim 4, which is characterized in that the gemma liquid preparation is prepared by the following method：
1) primary inclined plane seed：Beef extract-peptone slant medium is made, after being inoculated with bacillus megaterium BM22, culture is made
First order seed；
2) secondary liquid seed：Nutrition meat culture solution is contained in triangular flask under the control of logical oxygen, in sterile shape after high pressure sterilization
State is inoculated with the inclined-plane bacillus megaterium BM22 seed, and bacillus megaterium BM22 liquid seeds are made in shaken cultivation；
3) fermentation culture is contained in triangular flask under the control of logical oxygen after high pressure sterilization, is inoculated with huge gemma bar in germ-free condition
Gemma liquid preparation is made after inoculum concentration is the 15%, shaken cultivation 4 days of total liquid volume in bacterium BM22 liquid seeds.
7. application according to claim 6, which is characterized in that the beef extract-peptone slant medium is：Beef
Cream 5g, peptone 10g, NaCl 3.5g, agar 18g, water 1000mL.
8. application according to claim 6, which is characterized in that the nutrition meat culture solution is：Beef extract 7g, albumen
Peptone 10g, NaCl 5g, cyclamen root skin maceration extract 1000mL.
9. application according to claim 6, which is characterized in that the fermentation culture is：Maize flour 0.5-0.6%,
Cornstarch 0.08~0.2%, wheat bran 0.9~1.2%, glucose 0.18~0.54%, soybean cake powder 0.05~0.15%, greatly
Bean powder 0.2~0.5%, KH2PO40.005~0.006%, (NH4)2SO40.006~0.008%, MnSO40.004~
0.005%, CaCO30.0005~0.002%, MgSO4·7H2O 0.005~0.006% and the leaching of the cyclamen root of surplus
Stain liquid, pH 7.0~7.5.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114107071A (en) * | 2021-12-14 | 2022-03-01 | 山西省农业科学院园艺研究所 | Method for separating and purifying pathogenic bacteria of cyclamen wilt |
| CN114891852A (en) * | 2022-05-12 | 2022-08-12 | 广州萃普生物科技有限公司 | Method for obtaining high-content cyclamen saponin fermentation liquor, fermentation liquor and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103160455A (en) * | 2013-03-27 | 2013-06-19 | 杭州保安康生物技术有限公司 | Preparation method of spore preparation of bacillus coagulans |
| CN105875645A (en) * | 2015-12-23 | 2016-08-24 | 鹤壁市禾盛生物科技有限公司 | Preparing method of biological agent for preventing and controlling tobacco root black rot |
| CN107858304A (en) * | 2017-11-24 | 2018-03-30 | 四川农业大学 | A kind of Methylotrophic bacillus B18 and its liquid preparation and application |
-
2018
- 2018-07-06 CN CN201810738540.8A patent/CN108841752B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103160455A (en) * | 2013-03-27 | 2013-06-19 | 杭州保安康生物技术有限公司 | Preparation method of spore preparation of bacillus coagulans |
| CN105875645A (en) * | 2015-12-23 | 2016-08-24 | 鹤壁市禾盛生物科技有限公司 | Preparing method of biological agent for preventing and controlling tobacco root black rot |
| CN107858304A (en) * | 2017-11-24 | 2018-03-30 | 四川农业大学 | A kind of Methylotrophic bacillus B18 and its liquid preparation and application |
Non-Patent Citations (3)
| Title |
|---|
| 易龙 等: "烟草根黑腐病拮抗内生细菌的筛选及其抑菌作用", 《微生物学通报》 * |
| 朱天辉 主编: "《园林植物病理学》", 28 February 2003 * |
| 窦彦霞 等: "不同来源根串珠霉分子遗传多样性分析", 《中国植物病理学2015年学术年会论文集》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114107071A (en) * | 2021-12-14 | 2022-03-01 | 山西省农业科学院园艺研究所 | Method for separating and purifying pathogenic bacteria of cyclamen wilt |
| CN114891852A (en) * | 2022-05-12 | 2022-08-12 | 广州萃普生物科技有限公司 | Method for obtaining high-content cyclamen saponin fermentation liquor, fermentation liquor and application thereof |
| CN114891852B (en) * | 2022-05-12 | 2023-10-03 | 广州萃普生物科技有限公司 | Method for obtaining high-content cyclamen saponin fermentation liquor, fermentation liquor and application thereof |
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