CN108838158A - Micropore plate cleaning device for immunoassay experiment and use method - Google Patents
Micropore plate cleaning device for immunoassay experiment and use method Download PDFInfo
- Publication number
- CN108838158A CN108838158A CN201810778043.0A CN201810778043A CN108838158A CN 108838158 A CN108838158 A CN 108838158A CN 201810778043 A CN201810778043 A CN 201810778043A CN 108838158 A CN108838158 A CN 108838158A
- Authority
- CN
- China
- Prior art keywords
- rocker arm
- microwell plate
- motor
- filling head
- connect
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/08—Cleaning involving contact with liquid the liquid having chemical or dissolving effect
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/10—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration
Abstract
A cleaning device for a microporous plate used in an immunoassay experiment and a using method thereof. The automatic liquid filling machine comprises a machine frame (12), wherein the machine frame (12) is connected with a mixing motor (6) and a rocker arm motor (7) and is fixed through screws, the rocker arm motor (7) is connected with a rocker arm (2), the rocker arm (2) is connected with a rocker arm synchronizing wheel (11), the mixing motor (6) is connected with a mixing tray (5) with an eccentric wheel, the mixing tray (5) is connected with a magnet frame (4), the top of the machine frame (12) is connected with a liquid filling head moving motor (9), and the liquid filling head moving motor (9) is connected with a liquid filling head (3). The invention is used for cleaning the microporous plate in the immunoassay experiment.
Description
Technical field：
The present invention relates to a kind of microwell plate cleaning devices and application method in immunoassay experiment.
Background technique：
Immunoassay is to detect various substances using antigen and antibody specific association reaction（Drug, hormone, protein, micro- life
Object etc.）Analysis method；Enzyme-linked immune analytic method therein, chemiluminescence immune analysis method and polymerase chain reaction
In be directed to using microwell plate as reaction carriers in the form of, require the unbonded substance of cleaning removal in experimentation.It passes
The main wash mode of system is that injection needle filling washing lotion is impregnated, and then absorbs washing lotion with aspirating needle again.The shortcomings that this mode is：
If aspirating needle too far, may remain many washing lotions apart from bottom hole, if aspirating needle may closely touch very much apart from bottom hole
The conjugate of hole wall.Experimental result can be all influenced in this way, and in addition in the process of cleaning, can touch reaction liquid can also for aspirating needle
The consequence of cross contamination can be brought.It is that the centrifugal force generated using microwell plate rotation is removed washing lotion there are also a kind of cleaning method
Fall, such device solves only the removing problem of washing lotion, and the liquid that centrifugal force is thrown away will disperse surrounding, is unfavorable for clear
It is clean；Huge centrifugal force also makes the trim outstanding problem of load, and using effect is unsatisfactory in concrete application.
Summary of the invention：
The object of the present invention is to provide a kind of elimination effect consistency is good, debugging is simple, and easily operated one kind is for immune point
Microwell plate cleaning device and application method in analysis experiment.
Above-mentioned purpose is realized by following technical scheme：
A kind of microwell plate cleaning device in immunoassay experiment, composition include:Rack（12）, the rack（12）
Connection mixes motor（6）And Rocker arm-motor（7）And it is fixed by screw, the Rocker arm-motor（7）Connecting rocker arm（2）, described
Rocker arm（2）Connecting rocker arm synchronizing wheel（11）, the mixing motor（6）Connection has the mixing pallet of eccentric wheel（5）, described
Mixing pallet（5）Connect magnet frame（4）, the rack（12）Top connect filling head mobile motor（9）, the note
Liquid head mobile motor（9）Connect filling head（3）.
A kind of microwell plate cleaning device in immunoassay experiment, the rack（12）Connect waste liquid storehouse
（8）, the rack（12）Top connect shield（1）, the rack（12）Top connect washing lotion slot（11）, described
Magnet frame（4）Connect magnetic receiver.
A kind of microwell plate cleaning device in immunoassay experiment, the magnetic receiver includes pedestal
（13）, the pedestal（13）Connect left otic placode（14）With auris dextra plate（15）, the pedestal（13）The insertion magnet frame
（4）Cylindrical magnet, the pedestal（13）Connect microwell plate（16）, the pedestal（13）With the microwell plate（16）
It is provided with one group of micro- jack communicated（17）, micro- jack（17）Laterally it is parallel to each other, is vertically parallel to each other, the jack
（17）The corresponding filling head（3）.
A kind of application method for the microwell plate cleaning device in immunoassay experiment, rocker arm as shield side,
Pedestal and microwell plate are put into and mixed in pallet, by filling head mobile motor, filling head is moved on microwell plate, filling head
On have 8 injection needles, every fluid injection will once inject a certain amount of liquid into 8 holes, 12 times mobile in this way, fill 12 column,
96 holes of namely entire microwell plate；After filling integral plate, filling head is moved to initial position, and starting mixes motor, passes through
The eccentric wheel for mixing tray bottom is driven, the circular motion of entire microwell plate is realized, mixes well the liquid in micro- jack, stop
It only mixes, static certain time, if being the coating substance with magnetic particle in hole, magnetic frame can be close to the bottom of micropore,
Magnetic particle is firmly attracted to the bottom of micropore, then by rocker arm as on microwell plate, fixing rocker arm and microwell plate, micropore
Plate is also fixed together with magnet frame, is started Rocker arm-motor, rocker arm is quickly rotated 180 degree counterclockwise, the liquid meeting in microwell plate
It flow to the waste liquid storehouse of bottom with inertia, repeats the above process several times, unbonded substance is separated, the effect of cleaning is reached
Fruit.
A kind of application method for the microwell plate cleaning device in immunoassay experiment, during last
Rocker arm is rotated clockwise to certain angle, it is then ripe fastly to rotate by a certain angle counterclockwise, it is repeated several times, makes in micropore in this way
Residual liquid is less；Finally rocker arm 180 degree clockwise is unlocked locking device, cleaned by microwell plate as mixing in pallet
Journey is completed.
Beneficial effect：
1. syringe needle is not contacted with reaction liquid when fluid injection of the present invention, by rocker arm that microwell plate is quick without cross-contamination issue
Overturning, so that washing lotion be cleared out.
Filling head of the present invention is driven by filling head mobile motor, can carry out fluid injection to all holes inside microwell plate,
The position of fluid injection is accurate, and fluid injection effect is good.
The present invention solves in the prior art that residual liquid consistency is poor, destroys coating substance, aspirating needle haptoreaction liquid
Cross contamination caused by body and centrifugal device the problems such as structure is complicated, and stability is poor.
The rotation for the fast powerful that Rocker arm-motor of the invention is able to drive within the scope of rocker arm progress 180 degree facilitates cooperation to infuse
Liquid head uses, and using effect is good.
Detailed description of the invention：
Attached drawing 1 is the structural schematic diagram of this product.
Attached drawing 2 is the overall structure diagram of this product.
Attached drawing 3 is the structural schematic diagram of magnetic receiver in attached drawing 1.
Specific embodiment：
Below in conjunction with attached drawing of the invention, technical scheme in the embodiment of the invention is clearly and completely described.
Embodiment 1：
A kind of microwell plate cleaning device in immunoassay experiment, composition include:Rack 12, the rack 12 connect
It mixes motor 6 and Rocker arm-motor 7 and is fixed by screw, 7 connecting rocker arm 2 of Rocker arm-motor, the connection of rocker arm 2 is shaken
Arm synchronizing wheel 11, the mixing pallet 5 for mixing the connection of motor 6 and having eccentric wheel, the mixing pallet 5 connect magnet frame
4, the top of the rack 12 connects filling head mobile motor 9, and the filling head mobile motor 9 connects filling head 3.
Embodiment 2：
A kind of microwell plate cleaning device in immunoassay experiment described in embodiment 1, the rack 12 connect waste liquid
Storehouse 8, the top of the rack 12 connect shield 1, and the top of the rack 12 connects washing lotion slot 11, the magnet frame
4 connection magnetic receivers.
Embodiment 3：
A kind of microwell plate cleaning device in immunoassay experiment as described in example 2, the magnetic receiver includes pedestal
13, the pedestal 13 connects left otic placode 14 and auris dextra plate 15, and the pedestal 13 is inserted into the cylindrical type magnetic of the magnet frame 4
Iron, the pedestal 13 connect microwell plate 16, and the pedestal 13 is provided with one group of micro- jack communicated with the microwell plate 16
17, micro- jack 17 is laterally parallel to each other, is vertically parallel to each other, the corresponding filling head 3 of the jack 17.
Embodiment 4：
A kind of application method for the microwell plate cleaning device in immunoassay experiment, rocker arm is as shield side, the bottom of by
Seat and microwell plate, which are put into, to be mixed in pallet, and by filling head mobile motor, filling head is moved on microwell plate, is had on filling head
8 injection needles, every fluid injection will once inject a certain amount of liquid into 8 holes, 12 times mobile in this way, fill 12 column, also
It is 96 holes of entire microwell plate；After filling integral plate, filling head is moved to initial position, and starting mixes motor, passes through drive
The eccentric wheel for mixing tray bottom, realizes the circular motion of entire microwell plate, mixes well the liquid in micro- jack, stops mixed
Even, static certain time, if being the coating substance with magnetic particle in hole, magnetic frame can be close to the bottom of micropore, by magnetic
Particle is firmly attracted to the bottom of micropore,（If not containing the coating substance of magnetic particle, addition magnet frame can not had to）So
Rocker arm is also fixed as rocker arm and microwell plate, microwell plate on microwell plate, is fixed with magnet frame together afterwards, starting rocker arm electricity
Rocker arm is quickly rotated 180 degree by machine counterclockwise, and the liquid in microwell plate can flow to the waste liquid storehouse of bottom with inertia, is repeated above-mentioned
Process several times, unbonded substance is separated, achievees the effect that cleaning.
Embodiment 5：
A kind of application method for the microwell plate cleaning device in immunoassay experiment as described in example 4, in last mistake
Rocker arm is rotated clockwise to certain angle in journey（Generally less than 30 degree）, then ripe fastly to rotate by a certain angle counterclockwise（Micropore
Straight down）, it is repeated several times in this way, keeps the residual liquid in micropore less；Finally by rocker arm 180 degree clockwise, by microwell plate
As for mixing in pallet, locking device is unlocked, cleaning process is completed.
Embodiment 2：
A kind of microwell plate cleaning device in immunoassay experiment described in above-described embodiment, including filling head, filling head
Mobile motor, rocker arm, rocker motion motor, magnet frame mix pallet, the stepper motor for mixing（Mix motor）, protection
Cover, waste liquid box.
Wherein filling head is driven by filling head mobile motor, can carry out fluid injection to all holes inside microwell plate.
Wherein the quantity of the injection needle on filling head is not limited only to quantity shown in figure.
The rotation for the fast powerful that wherein Rocker arm-motor can drive rocker arm to carry out within the scope of 180 degree.
Motor is wherein mixed by eccentric wheel structure drive pallet, can make liquid in each of microwell plate hole into
The mixing of row Scroll-tupe.
Wherein arranged and the consistent magnetic column of microwell plate hole count, the top of each magnetic column and microwell plate inside magnet frame
Bottom hole is to be close to, and the diameter of the two is close.
Wherein magnet frame is only applicable in the method for magnetic particle coating technique, and magnet can not be used in other forms
Frame.
The wherein cylindrical magnet quantity in the hole count in microwell plate and corresponding magnetic receiver, is not limited only to 96,
It is also applied for other quantity.
Wherein the number of teeth of synchronous rocker wheel is greater than the synchronization tooth number of Rocker arm-motor, the purpose for the arrangement is that increasing rocker arm
Torsion keeps washing lotion separation more thorough.
Claims (5)
1. a kind of microwell plate cleaning device in immunoassay experiment, composition include:Rack（12）, it is characterized in that:Institute
The rack stated（12）Connection mixes motor（6）And Rocker arm-motor（7）And it is fixed by screw, the Rocker arm-motor（7）Connection
Rocker arm（2）, the rocker arm（2）Connecting rocker arm synchronizing wheel（11）, the mixing motor（6）Connection has the mixing of eccentric wheel
Pallet（5）, the mixing pallet（5）Connect magnet frame（4）, the rack（12）Top connect filling head mobile motor
（9）, the filling head mobile motor（9）Connect filling head（3）.
2. a kind of microwell plate cleaning device in immunoassay experiment according to claim 1, it is characterized in that:Institute
The rack stated（12）Connect waste liquid storehouse（8）, the rack（12）Top connect shield（1）, the rack（12）'s
Top connects washing lotion slot（11）, the magnet frame（4）Connect magnetic receiver.
3. a kind of microwell plate cleaning device in immunoassay experiment according to claim 2, it is characterized in that:Institute
The magnetic receiver stated includes pedestal（13）, the pedestal（13）Connect left otic placode（14）With auris dextra plate（15）, the pedestal
（13）The insertion magnet frame（4）Cylindrical magnet, the pedestal（13）Connect microwell plate（16）, the pedestal
（13）With the microwell plate（16）It is provided with one group of micro- jack communicated（17）, micro- jack（17）Laterally be parallel to each other,
Vertically it is parallel to each other, the jack（17）The corresponding filling head（3）.
4. a kind of application method for the microwell plate cleaning device in immunoassay experiment, it is characterized in that:Rocker arm is as protection
Side is covered, pedestal and microwell plate are put into and mixed in pallet, by filling head mobile motor, filling head is moved to microwell plate
On, there are 8 injection needles on filling head, every fluid injection will once inject a certain amount of liquid into 8 holes, and it is 12 times mobile in this way,
Fill 12 column, that is, 96 holes of entire microwell plate；After filling integral plate, filling head is moved to initial position, and starting mixes
Motor mixes the eccentric wheel of tray bottom by driving, realizes the circular motion of entire microwell plate, fill the liquid in micro- jack
Divide and mix, stops mixing, static certain time, if being the coating substance with magnetic particle in hole, magnetic frame can be close to micro-
Magnetic particle is firmly attracted to the bottom of micropore by the bottom in hole, then by rocker arm as on microwell plate, fix rocker arm with it is micro-
Orifice plate, microwell plate are also fixed together with magnet frame, start Rocker arm-motor, and rocker arm is quickly rotated 180 degree counterclockwise, in microwell plate
Liquid the waste liquid storehouse of bottom can be flow to inertia, repeat the above process several times, unbonded substance separated, reaches clear
The effect washed.
5. a kind of application method for the microwell plate cleaning device in immunoassay experiment according to claim 4,
It is characterized in:Rocker arm is rotated clockwise to certain angle during last, it is then ripe fastly to rotate by a certain angle counterclockwise, this
Sample is repeated several times, and keeps the residual liquid in micropore less；Finally by rocker arm 180 degree clockwise, by microwell plate as mixing pallet
In, locking device is unlocked, cleaning process is completed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810778043.0A CN108838158A (en) | 2018-07-16 | 2018-07-16 | Micropore plate cleaning device for immunoassay experiment and use method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810778043.0A CN108838158A (en) | 2018-07-16 | 2018-07-16 | Micropore plate cleaning device for immunoassay experiment and use method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108838158A true CN108838158A (en) | 2018-11-20 |
Family
ID=64197700
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810778043.0A Pending CN108838158A (en) | 2018-07-16 | 2018-07-16 | Micropore plate cleaning device for immunoassay experiment and use method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108838158A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0712821A (en) * | 1993-06-24 | 1995-01-17 | Matsushita Electric Ind Co Ltd | Sample mixing device and fluorescence detector using the device |
| CN2463077Y (en) * | 2001-01-22 | 2001-12-05 | 上海新波生物技术有限公司 | Motion machinery structure of automatic microporous washing machine |
| CN101319976A (en) * | 2008-07-03 | 2008-12-10 | 清华大学 | Micro-pore plate type electromagnetic separator |
| CN202155327U (en) * | 2011-05-18 | 2012-03-07 | 闫国超 | ELISA plate washing machine |
| CN202943067U (en) * | 2012-12-18 | 2013-05-22 | 博奥赛斯（天津）生物科技有限公司 | Microporous plate washing and dehydrating device |
| CN105092867A (en) * | 2015-09-11 | 2015-11-25 | 苏州浩欧博生物医药有限公司 | Full-automatic integrated structure for treating magnetic particles in chemical luminescence immune assay |
| CN106925559A (en) * | 2017-03-10 | 2017-07-07 | 上海市儿童医院 | A kind of micropore cleaning device for EUSA |
| CN208810703U (en) * | 2018-07-16 | 2019-05-03 | 精准（天津）生物科技有限公司 | A micropore board belt cleaning device for among immunoassay experiment |
-
2018
- 2018-07-16 CN CN201810778043.0A patent/CN108838158A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0712821A (en) * | 1993-06-24 | 1995-01-17 | Matsushita Electric Ind Co Ltd | Sample mixing device and fluorescence detector using the device |
| CN2463077Y (en) * | 2001-01-22 | 2001-12-05 | 上海新波生物技术有限公司 | Motion machinery structure of automatic microporous washing machine |
| CN101319976A (en) * | 2008-07-03 | 2008-12-10 | 清华大学 | Micro-pore plate type electromagnetic separator |
| CN202155327U (en) * | 2011-05-18 | 2012-03-07 | 闫国超 | ELISA plate washing machine |
| CN202943067U (en) * | 2012-12-18 | 2013-05-22 | 博奥赛斯（天津）生物科技有限公司 | Microporous plate washing and dehydrating device |
| CN105092867A (en) * | 2015-09-11 | 2015-11-25 | 苏州浩欧博生物医药有限公司 | Full-automatic integrated structure for treating magnetic particles in chemical luminescence immune assay |
| CN106925559A (en) * | 2017-03-10 | 2017-07-07 | 上海市儿童医院 | A kind of micropore cleaning device for EUSA |
| CN208810703U (en) * | 2018-07-16 | 2019-05-03 | 精准（天津）生物科技有限公司 | A micropore board belt cleaning device for among immunoassay experiment |
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