CN108822068A - A method of preparing wild blueberry anthocyanidin - Google Patents
A method of preparing wild blueberry anthocyanidin Download PDFInfo
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- CN108822068A CN108822068A CN201810562891.8A CN201810562891A CN108822068A CN 108822068 A CN108822068 A CN 108822068A CN 201810562891 A CN201810562891 A CN 201810562891A CN 108822068 A CN108822068 A CN 108822068A
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- blueberry
- pomace
- temperature
- anthocyanidin
- raw material
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
Abstract
The invention discloses a kind of method for preparing wild blueberry anthocyanidin, method is:Remaining pomace is granulated after ensiling, drying, crushing after blueberry is squeezed the juice, then blueberry pomace particle is subjected to low temperature extraction by solvent of ethyl alcohol, it is handled again using equipment such as filtering, centrifugations, then concentration is carried out to treatment fluid using ceramic membrane and nanofiltration membrane, concentrate is concentrated using purification with macroreticular resin, molecular distillation, is finally freezed to obtain anthocyanidin product again.The present invention uses remaining pomace for raw material, can adequately utilize resource;Blueberry pomace is granulated, greatly extend raw material storage time, effectively avoid pomace because extraction not in time due to cause to rot;Low temperature extraction can effectively reduce influence of the Extracting temperature to anthocyanidin, reduce content loss;Of the invention entire extraction, concentration, purifying, drying process carry out at a lower temperature, while reducing high temperature influences anthocyanidin, also reduce the use to the energy, improving yield reduces cost.
Description
Technical field
The invention belongs to field of natural organic chemistry, it is related to remaining pomace after one kind is squeezed the juice using blueberry and is spent as raw material preparation
The method of green element.
Background technique
Blueberry (Blueberry) scientific name blueberry, belongs to Ericaceae blueberry platymiscium, is grown on to the north of China Daxing'an Mountainrange
Blueberry among virgin forest is referred to as " beep persimmon ", and blueberry can resist subzero 50 DEG C of severe cold, and root is grown in frozen soil throughout the year,
Fruit is blue, and pulp is fine and smooth, and fruity is sour-sweet, unique flavor, full of nutrition, and it is rare rare wild fruit that value is high.
Anthocyan ingredient is rich in blueberry.Anthocyanidin is a kind of water colo(u)r, can be changed with the soda acid of cell liquid
Become color.For cell liquid in acid then partially red, cell liquid is then partially blue in alkalinity, and anthocyanidin is constitute petal and fruit color main
One of pigment.Anthocyanidin is the nutritional supplement of pure natural anti-aging physiologically playing the part of important role, anthocyanidin, research
It is proved to be mankind nowadays and finds that most effective anioxidant phytochemicals, its antioxygenic property are higher by 50 times than vitamin E, than dimension
Raw element C is higher by 20 times.It is 100% to the biological effectiveness of human body, can be detected in blood within 20 minutes after taking,
To enhancing eyesight, anti-eye strain, cranial nerve aging is delayed to have significant effect, while to the blood capillary as caused by diabetes
Pipe disease also has preferable therapeutic effect, moreover it is possible to enhance a variety of diseases such as cardio-pulmonary function, prevention senile dementia.
The raw material blueberry resource that all comes from blueberry, but utilize of anthocyanidin in blueberry but phase not to the utmost is extracted at present
Together, some is using blueberry fresh fruit as raw material, and some is using blueberry freeze fruit as raw material, and some is using blueberry pomace as raw material, and some is with blueberry
Fruit juice is raw material.Anthocyanidin is extracted using blueberry fresh fruit or blueberry freeze fruit as raw material, is the main method for preparing anthocyanidin at present, is
Vast manufacturing enterprise is used.Mainly blueberry is squeezed the juice in the method that blueberry juice prepares anthocyanidin as raw material, then utilizes indigo plant
Certain kind of berries juice prepares anthocyanidin, such as the Chinese patent of 106380458 A of publication number CN, discloses a kind of extraction of anthocyanidin in blueberry
Method solves conventional organic solvents extraction method and needs higher Extracting temperature, be easy to cause part thermal sensitivity in extraction process
The loss of ingredient, and organic solvent is easy the problems such as remaining, dissolving poor selectivity.The insufficient place of this method is to remain after squeezing the juice
The waste of resource also is be easy to cause if cannot efficiently use containing a large amount of anthocyan substance in remaining blueberry pomace.With
Blueberry pomace is the Chinese patent for method such as 106349205 A of publication number CN that raw material prepares anthocyanidin, describes one kind to squeeze
Blueberry pomace after juice is the method that raw material prepares anthocyanidin, and it is insufficient to solve blueberry anthocyanin DNA purity in the prior art
Technical problem.But this method also has insufficient place, if remaining blueberry pomace cannot be used for blueberry flower as early as possible after juicing
The extraction of green element, can generate phenomena such as rancid of rotting after placing a period of time, will affect the quality for extracting product blueberry anthocyanin,
It will also result in the waste of resource.
Mainly there are solvent extraction method, microwave method, supercritical fluid extraction etc. to the research of the extraction of anthocyanidin at present, one
As be all to be extracted under the conditions of temperature above room temperature, such as the Chinese patent of 101914304 A of Publication No. CN, use
Extracting temperature be 40~65 DEG C;The Chinese patent of 106975014 A of Publication No. CN, Extracting temperature are 35~40 DEG C.Cyanine
Plain class belongs to heat-sensitive substance, and high temperature easily causes its content loss, therefore easily takes low temperature to carry out when extraction.
Summary of the invention
During overcoming using blueberry pomace as raw material extraction blueberry anthocyanin, due to raw material indigo plant
What certain kind of berries pomace used causes the production processes such as the wasting of resources for rotting rancid and high temperature extraction that anthocyanidin is caused to lose not in time
The shortcomings that, provide it is a kind of be granulated using pomace, low temperature extracts, the blueberry anthocyanin preparation method of cryogenic purincation.
The present invention after blueberry is squeezed the juice first remaining pomace be granulated after ensiling, drying, crushing, then by blueberry
Pomace particle carries out low temperature extraction by solvent of ethyl alcohol, then is handled using equipment such as filtering, centrifugations, and ceramic membrane is then used
With nanofiltration membrane to treatment fluid carry out concentration, concentrate using purification with macroreticular resin, molecular distillation be concentrated, finally again into
Row freezing obtains the blueberry extract product containing anthocyanidin.
The technical proposal of the invention is realized in this way, it includes the following steps:
(1)Remaining pomace puts it into silage silo as raw material after being squeezed the juice using blueberry, and a certain amount of ensiling agent is added and is store
Hiding;
(2)By step(1)Raw material after ensiling is dried;
(3)By step(2)Obtained dry raw material is crushed, and is then granulated, and pomace particle is obtained;
(4)By step(3)Obtained in particle be put into extraction tank, using ethyl alcohol as Extraction solvent, low-temperature circulating extract;
(5)By step(4)After obtained extracting solution is using the equipment removal large granular impurity such as filtering, centrifugation, successively using ceramics
Film and nanofiltration membrane carry out concentration, obtain concentrate;
(6)By step(5)Obtained concentrate is purified using macroporous absorbent resin, collects the eluent containing anthocyanidin;
(7)By step(6)The eluent of collection is concentrated using molecular distillation, obtains concentrate;
(8)By step(7)Obtained concentrate carries out vacuum freeze drying, obtains the finished powder containing anthocyanidin.
The preferable technical solution of the present invention is:In the step (1), the main component of ensiling agent is lactic acid bacteria, can also
Pectase, cellulase etc. are added on this basis.The usage amount of ensiling agent be blueberry pomace per ton be added ensiling agent 10~
20g, every 100g ensiling agent are uniformly sprayed on blueberry pomace surface after dissolving activation with 20kg clear water, the ensiling time is 80~96 small
When.
The preferable technical solution of the present invention is:The step(2)In, it is dried using flotation dryer, hot-blast stove provides
Hot wind, hot blast temperature are 220~250 DEG C, and dry fruit pulp water point is less than 10%.
The preferable technical solution of the present invention is:The step(3)In, raw material is crushed to mesh number less than 40 mesh, and use is cold
Wind cools down to particle.
The preferable technical solution of the present invention is:The step(4)In, the ratio of particle and ethyl alcohol is 1:3~4(m/v),
The volumetric concentration of ethanol solution is 80~90%, and the temperature that low temperature extracts is 10~15 DEG C, and circulation is extracted, and is extracted 2 times, every all over 60
Minute.
The preferable technical solution of the present invention is:The step(5)In, the film of 150~200nm is selected in the aperture of ceramic membrane
Element, the temperature of ceramic membrane treatment fluid are 15~20 DEG C, and pressure is 0.2~0.4MPa, and membrane flux is 60~100L/m2*h.It receives
The molecular cut off of filter membrane selects 200~400Dal, and the temperature of nanofiltration membrane treatment liquid is 15~20 DEG C, and pressure is 0.3~
0.5MPa, membrane flux are 8~12L/m2*h.The volumetric concentration of ethanol solution is less than 1% in obtained concentrate.
The preferable technical solution of the present invention is:The step(7)In, the pressure of molecular distillation is 20~30Pa, evaporation
Temperature be 30~35 DEG C, 0~10 DEG C of condensation temperature, 1.0~2.0L/min of material flow.
The preferable technical solution of the present invention is:The step(8)In, control vacuum degree is in 10~13Pa, temperature when freezing
Degree is -50~-40 DEG C, and freeze-drying is to moisture content below 5%.
Advantages of the present invention has the following aspects:
(1)Remaining pomace can adequately utilize resource, reduce the wave of resource after the present invention uses blueberry to squeeze the juice for starting material
Take;
(2)Blueberry pomace is granulated by the present invention, can greatly extend the storage time of raw material, effectively avoid blueberry pomace because
That extracts causes to rot not in time, reduces the waste of resource and reduces the pollution of environment;
(3)The present invention is extracted using low temperature, can effectively reduce influence of the Extracting temperature to anthocyan substance, reduces content
Loss;
(4)Of the invention entire extraction, concentration, purifying, drying process carry out at a lower temperature as far as possible, can reduce height
While temperature influences anthocyan substance, moreover it is possible to reduce the use to the energy, improve yield, reduce cost.
Specific embodiment
Embodiment 1
The ensiling agent of 1.5g is added, after ensiling agent 0.3kg clear water dissolution activation uniformly in pomace 100kg after taking blueberry to squeeze the juice
It is sprayed on blueberry pomace surface, pomace is covered with black plastic foil after seething uniformly, then covers straw mat, weight in pressure, when ensiling
Between be 90 hours.Blueberry pomace after ensiling is dried using flotation dryer, and hot-blast stove provides hot wind, and hot blast temperature is
230 ± 5 DEG C, air blower air-supply, moisture is 8.7% in pomace after drying.Dry fruit is collected using feeder in flotation dryer outlet
Slag send to pulverizer through closed conduit and carries out being crushed to 40 mesh, and blueberry pomace then is made through pipeline to granulator
Grain, then cooled down using cold wind to particle, obtain blueberry pomace particle 58.05kg.
Blueberry pomace particle 58.05kg is put into extractor, 200 liters of ethanol solution, 10 DEG C that volumetric concentration is 80% are added
Under conditions of low temperature extract, the liquid in extractor is extracted out from the tap hole of extractor using diaphragm pump, then from extractor
Filling opening is squeezed into, and circulation is extracted, and extraction time is 60 minutes, collects extracting solution.Then according still further to said extracted process and parameter
It extracts 1 time again.It extracts 2 times in total, and 2 times extracting solutions is merged into obtain 375L solution.
Extracting solution after merging is first filtered using plate filter(200 mesh of filter cloth), then carried out using ceramic membrane
The membrane component of 150nm is selected in refined filtration, the aperture of ceramic membrane, and the temperature for the treatment of fluid is 15 DEG C, pressure 0.2MPa, and membrane flux is
60L/m2*h collects ceramic membrane permeate.Then ceramic membrane permeate is concentrated using nanofiltration membrane, the retention point of nanofiltration membrane
Son amount selection 200Dal, the temperature for the treatment of fluid is 15 DEG C, pressure 0.3MPa, membrane flux 8L/m2*h.By repeatedly to concentration
Crossing nanofiltration membrane after addition purified water in liquid makes the percentage by volume of ethanol solution in finally obtained concentrate less than 1%, obtains
10.74kg concentrate.Concentrate is purified using macroporous absorbent resin, collects the ethanol eluate containing anthocyanidin.By second
Alcohol eluen is distilled using molecular distillation equipment, and the pressure of molecular distillation is 20Pa, and evaporating temperature is 30 DEG C, condensation temperature
0 DEG C, material flow 1.0L/min, obtain molecular distillation concentrate 2.08kg.It is cold that molecular distillation concentrate is finally put into vacuum
It is dried in lyophilizer, control vacuum degree is 10Pa, and temperature is -50 DEG C, and freeze-drying to moisture content is 4.22%.?
Weight to blueberry pomace extract is 0.625kg, and wherein the purity of anthocyanidin is 38.41%.
Embodiment 2
The ensiling agent of 2.0g is added, after ensiling agent 0.4kg clear water dissolution activation uniformly in pomace 100kg after taking blueberry to squeeze the juice
It is sprayed on blueberry pomace surface, pomace is covered with black plastic foil after seething uniformly, then covers straw mat, weight in pressure, when ensiling
Between be 80 hours.Blueberry pomace after ensiling is dried using flotation dryer, and hot-blast stove provides hot wind, and hot blast temperature is
240 ± 5 DEG C, air blower air-supply, moisture is 8.4% in pomace after drying.Dry fruit is collected using feeder in flotation dryer outlet
Slag send to pulverizer through closed conduit and carries out being crushed to 30 mesh, and blueberry pomace then is made through pipeline to granulator
Grain, then cooled down using cold wind to particle, obtain blueberry pomace particle 57.86kg.
Blueberry pomace particle 57.86kg is put into extractor, 230 liters of ethanol solution, 15 DEG C that volumetric concentration is 90% are added
Under conditions of low temperature extract, the liquid in extractor is extracted out from the tap hole of extractor using diaphragm pump, then from extractor
Filling opening is squeezed into, and circulation is extracted, and extraction time is 60 minutes, collects extracting solution.Then according still further to said extracted process and parameter
It extracts 1 time again.It extracts 2 times in total, and 2 times extracting solutions is merged into obtain 433L solution.
Extracting solution after merging is first filtered using plate filter(200 mesh of filter cloth), then carried out using ceramic membrane
The membrane component of 200nm is selected in refined filtration, the aperture of ceramic membrane, and the temperature for the treatment of fluid is 18 DEG C, pressure 0.3MPa, and membrane flux is
80L/m2*h collects ceramic membrane permeate.Then ceramic membrane permeate is concentrated using nanofiltration membrane, the retention point of nanofiltration membrane
Son amount selection 300Dal, the temperature for the treatment of fluid is 18 DEG C, pressure 0.4MPa, membrane flux 10L/m2*h.By repeatedly to dense
Crossing nanofiltration membrane after addition purified water in contracting liquid makes the percentage by volume of ethanol solution in finally obtained concentrate less than 1%, obtains
11.12kg concentrate.Concentrate is purified using macroporous absorbent resin, collects the ethanol eluate containing anthocyanidin.By second
Alcohol eluen is distilled using molecular distillation equipment, and the pressure of molecular distillation is 30Pa, and evaporating temperature is 35 DEG C, condensation temperature
10 DEG C, material flow 2.0L/min, obtain molecular distillation concentrate 2.31kg.It is cold that molecular distillation concentrate is finally put into vacuum
It is dried in lyophilizer, control vacuum degree is 13Pa, and temperature is -40 DEG C, and freeze-drying to moisture content is 4.18%.?
Weight to blueberry pomace extract is 0.651kg, and wherein the purity of anthocyanidin is 37.06%.
Embodiment 3
The ensiling agent of 1.2g is added, after ensiling agent 0.24kg clear water dissolution activation uniformly in pomace 100kg after taking blueberry to squeeze the juice
It is sprayed on blueberry pomace surface, pomace is covered with black plastic foil after seething uniformly, then covers straw mat, weight in pressure, when ensiling
Between be 96 hours.Blueberry pomace after ensiling is dried using flotation dryer, and hot-blast stove provides hot wind, and hot blast temperature is
220 ± 5 DEG C, air blower air-supply, moisture is 9.4% in pomace after drying.Dry fruit is collected using feeder in flotation dryer outlet
Slag send to pulverizer through closed conduit and carries out being crushed to 40 mesh, and blueberry pomace then is made through pipeline to granulator
Grain, then cooled down using cold wind to particle, obtain blueberry pomace particle 58.50kg.
Blueberry pomace particle 58.50kg is put into extractor, 180 liters of ethanol solution, 13 DEG C that volumetric concentration is 85% are added
Under conditions of low temperature extract, the liquid in extractor is extracted out from the tap hole of extractor using diaphragm pump, then from extractor
Filling opening is squeezed into, and circulation is extracted, and extraction time is 60 minutes, collects extracting solution.Then according still further to said extracted process and parameter
It extracts 1 time again.It extracts 2 times in total, and 2 times extracting solutions is merged into obtain 338L solution.
Extracting solution after merging is first filtered using plate filter(200 mesh of filter cloth), then carried out using ceramic membrane
The membrane component of 200nm is selected in refined filtration, the aperture of ceramic membrane, and the temperature for the treatment of fluid is 20 DEG C, pressure 0.4MPa, and membrane flux is
100L/m2*h collects ceramic membrane permeate.Then ceramic membrane permeate is concentrated using nanofiltration membrane, the retention of nanofiltration membrane
Molecular weight selects 400Dal, and the temperature for the treatment of fluid is 20 DEG C, pressure 0.5MPa, membrane flux 12L/m2*h.By repeatedly to
Crossing nanofiltration membrane after addition purified water in concentrate makes the percentage by volume of ethanol solution in finally obtained concentrate less than 1%, obtains
To 10.98kg concentrate.Concentrate is purified using macroporous absorbent resin, collects the ethanol eluate containing anthocyanidin.It will
Ethanol eluate is distilled using molecular distillation equipment, and the pressure of molecular distillation is 25Pa, and evaporating temperature is 33 DEG C, condensation temperature
5 DEG C, material flow 1.5L/min of degree, obtains molecular distillation concentrate 2.11kg.Molecular distillation concentrate is finally put into vacuum
It is dried in freeze drier, control vacuum degree is 10Pa, and temperature is -50 DEG C, and freeze-drying to moisture content is 4.59%.
The weight for obtaining blueberry pomace extract is 0.629kg, and wherein the purity of anthocyanidin is 38.23%.
Claims (11)
1. a kind of method for preparing wild blueberry anthocyanidin, which is characterized in that including following steps:
(1)Remaining pomace puts it into silage silo as raw material after being squeezed the juice using blueberry, and a certain amount of ensiling agent is added and is store
Hiding;
(2)By step(1)Raw material after ensiling is dried;
(3)By step(2)Obtained dry raw material is crushed, and is then granulated, and pomace particle is obtained;
(4)By step(3)Obtained in particle be put into extraction tank, using ethyl alcohol as Extraction solvent, low-temperature circulating extract;
(5)By step(4)After obtained extracting solution is using the equipment removal large granular impurity such as filtering, centrifugation, successively using ceramics
Film and nanofiltration membrane carry out concentration, obtain concentrate;
(6)By step(5)Obtained concentrate is purified using macroporous absorbent resin, collects the eluent containing anthocyanidin;
(7)By step(6)The eluent of collection is concentrated using molecular distillation, obtains concentrate;
(8)By step(7)Obtained concentrate carries out vacuum freeze drying, obtains the finished powder containing anthocyanidin.
2. a kind of method for preparing wild blueberry anthocyanidin according to claim 1, it is characterised in that:The step
(1) in, the main component of ensiling agent is lactic acid bacteria, can also add pectase, cellulase etc. on this basis.
3. the usage amount of ensiling agent is that 10~20g of ensiling agent is added in blueberry pomace per ton, every 100g ensiling agent is molten with 20kg clear water
Blueberry pomace surface is uniformly sprayed on after solution activation, the ensiling time is 80~96 hours.
4. a kind of method for preparing wild blueberry anthocyanidin according to claim 1, it is characterised in that:The step
(2)In, it is dried using flotation dryer, hot-blast stove provides hot wind, and hot blast temperature is 220~250 DEG C, and dry fruit pulp water point is small
In 10%.
5. a kind of method for preparing wild blueberry anthocyanidin according to claim 1, it is characterised in that:The step
(3)In, raw material is crushed to mesh number less than 40 mesh, is cooled down using cold wind to particle.
6. a kind of method for preparing wild blueberry anthocyanidin according to claim 1, it is characterised in that:The step
(4)In, the ratio of particle and ethyl alcohol is 1:3~4(m/v), the volumetric concentration of ethanol solution is 80~90%, the temperature that low temperature extracts
Degree is 10~15 DEG C, and circulation is extracted, and is extracted 2 times, every all over 60 minutes.
7. a kind of method for preparing wild blueberry anthocyanidin according to claim 1, it is characterised in that:The step
(5)In, the membrane component of 150~200nm is selected in the aperture of ceramic membrane, and the temperature of ceramic membrane treatment fluid is 15~20 DEG C, and pressure is
0.2~0.4MPa, membrane flux are 60~100L/m2*h。
8. the molecular cut off of nanofiltration membrane selects 200~400Dal, the temperature of nanofiltration membrane treatment liquid is 15~20 DEG C, and pressure is
0.3~0.5MPa, membrane flux are 8~12L/m2*h。
9. the volumetric concentration of ethanol solution is less than 1% in obtained concentrate.
10. a kind of method for preparing wild blueberry anthocyanidin according to claim 1, it is characterised in that:The step
(7)In, the pressure of molecular distillation is 20~30Pa, and evaporating temperature is 30~35 DEG C, 0~10 DEG C of condensation temperature, material flow 1.0
~2.0L/min.
11. a kind of method for preparing wild blueberry anthocyanidin according to claim 1, it is characterised in that:The step
(8)In, for control vacuum degree in 10~13Pa, temperature is -50~-40 DEG C when freezing, freeze-drying to moisture content 5% with
Under.
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN109293618A (en) * | 2018-11-30 | 2019-02-01 | 黑龙江绿知都生物科技开发有限公司 | A kind of blueberry anthocyanin isolation and purification method |
CN110237226A (en) * | 2019-07-12 | 2019-09-17 | 四平三帆科技制药有限公司 | A kind of preparation method of traditional Chinese medicine plaster pain-relieving plaster for injury and rheumatism |
CN110669035A (en) * | 2019-10-07 | 2020-01-10 | 大兴安岭至臻尚品寒带生物技术有限公司 | Method for preparing anthocyanin from indigo fruit |
CN115466239A (en) * | 2022-09-02 | 2022-12-13 | 贵州成有王记善沅食品有限公司 | Extraction method of high-purity anthocyanin |
WO2022270684A1 (en) * | 2021-06-22 | 2022-12-29 | 주식회사 세이브앤코 | Method for extracting anthocyanin components for improving urinary tract health by using plant lactic acid bacteria and cranberries for promoting female reproductive health, and extract thereby |
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2018
- 2018-06-04 CN CN201810562891.8A patent/CN108822068A/en active Pending
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109293618A (en) * | 2018-11-30 | 2019-02-01 | 黑龙江绿知都生物科技开发有限公司 | A kind of blueberry anthocyanin isolation and purification method |
CN109293618B (en) * | 2018-11-30 | 2022-06-17 | 黑龙江绿知都生物科技开发有限公司 | Blueberry anthocyanin separation and purification method |
CN110237226A (en) * | 2019-07-12 | 2019-09-17 | 四平三帆科技制药有限公司 | A kind of preparation method of traditional Chinese medicine plaster pain-relieving plaster for injury and rheumatism |
CN110669035A (en) * | 2019-10-07 | 2020-01-10 | 大兴安岭至臻尚品寒带生物技术有限公司 | Method for preparing anthocyanin from indigo fruit |
WO2022270684A1 (en) * | 2021-06-22 | 2022-12-29 | 주식회사 세이브앤코 | Method for extracting anthocyanin components for improving urinary tract health by using plant lactic acid bacteria and cranberries for promoting female reproductive health, and extract thereby |
CN115466239A (en) * | 2022-09-02 | 2022-12-13 | 贵州成有王记善沅食品有限公司 | Extraction method of high-purity anthocyanin |
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