CN108815291B

CN108815291B - Composition for reducing body fat rate and preparation thereof

Info

Publication number: CN108815291B
Application number: CN201810713822.2A
Authority: CN
Inventors: 钱景; 杨敏; 刘雳; 瞿海斌; 程翼宇; 顾佳丽
Original assignee: CHIATAI QINGCHUNBAO PHARMACEUTICAL CO LTD
Current assignee: CHIATAI QINGCHUNBAO PHARMACEUTICAL CO LTD
Priority date: 2018-07-03
Filing date: 2018-07-03
Publication date: 2021-06-11
Anticipated expiration: 2038-07-03
Also published as: CN108815291A

Abstract

The invention discloses a traditional Chinese medicine composition and a preparation thereof, wherein the traditional Chinese medicine composition at least comprises the following components in parts by weight: 14-18 parts of radix rehmanniae recen, 4-8 parts of red ginseng and 2-4 parts of poria cocos. The traditional Chinese medicine composition can be prepared into one of the following pharmaceutical preparations after being added with pharmaceutically acceptable auxiliary materials: tablet, granule, capsule, oral liquid or soft extract. The traditional Chinese medicine composition and the preparation containing the composition can reduce the fat percentage of obese people, improve the body composition, increase the ratio of brown fat/white fat, and have positive effects on glycolipid metabolic disturbance accompanied by obesity.

Description

Composition for reducing body fat rate and preparation thereof

One, the technical field

The invention relates to a traditional Chinese medicine composition for reducing body fat rate and a preparation thereof.

Second, background Art

The development of the traditional Chinese medicine is improved for 30 years, the economic development of China is rapid, the living standard of people is increasingly improved, and the number of overweight and diabetic patients is greatly increased. The report of nutrition and chronic disease conditions of Chinese residents in 2015 shows that the overweight rate of adults 18 years old and older in China exceeds 30.1%, the obesity rate exceeds 11.9% and the diabetes prevalence rate reaches 11.6% (more than 90% of the adults belong to type II diabetes). It is not accurate to judge whether obesity is caused by weight or BMI value. To determine whether obesity is present, the criteria should be body fat percentage, i.e., the percentage of body fat in total body weight. Fat is essential for human body constitution, and too much or too little can affect health. For men, about 3-4% of body fat is essential fat, and for women, about 10-12% of body fat is essential fat, which affects health. While men are more than 25% and women are obese for more than 35%. The two components constituting body fat are subcutaneous fat and visceral fat, both of which are collectively called white fat. The former affects the appearance, and the latter surrounds organs such as the liver, which is the most dangerous fat. Obesity is often accompanied by insulin resistance, metabolic syndrome, cardiovascular disease, and the like due to the accumulation of large amounts of white fat. In recent years, studies have found that brown fat is still present in adults. Brown adipocytes have its uniqueness in terms of gene, structure and function compared to white fat. Mitochondria in cells in white adipose tissues are few, fat is stored in a form of single-chamber large fat droplets, and the fat storage device has the main function of serving as an energy source bank of an organism and storing energy for the organism; the brown adipose tissue cells contain a large number of mitochondria, store fat in the form of multi-cavity small fat droplets, and have the main function of maintaining the body temperature through a non-shivering form, namely burning fat to generate heat when the body is cold so as to adapt to the environment. The classical brown fat-regulated energy metabolism has important significance for regulating the blood sugar level and the blood fat level of the organism. Studies have shown that increasing the number and function of mouse brown adipocytes helps promote a lean, healthy phenotype.

At present, the products for losing weight on the market are various. Some limit caloric intake by controlling appetite, increasing satiety, but are prone to malnutrition if scientific guidance is lacking; some of the traditional Chinese medicine composition achieves the purpose of losing weight by promoting diarrhea, and has greater harm to body health; the fat-reducing drugs approved by the state on the market belong to lipase inhibitors, can only inhibit the digestion and absorption of fat, but can possibly cause insufficient intake of essential fatty acid. It is considered that obesity is caused by the fact that calories ingested exceed the metabolic needs of the body and are accumulated in the body in the form of fat, as is required for daily activities. The metabolic mode of the organism is regulated, so that on one hand, the metabolism can be promoted, and the redundant capacity can be consumed; on the other hand, the fat type is adjusted, the proportion of brown fat is increased, the proportion of white fat is reduced, and the purposes of losing weight and increasing health and benefiting benefit can be achieved.

Third, the invention

The invention aims to provide a traditional Chinese medicine composition for reducing body fat rate.

The technical scheme adopted by the invention is as follows:

a traditional Chinese medicine composition is characterized in that: the traditional Chinese medicine composition is at least prepared from the following raw materials in parts by weight: 14-18 parts of radix rehmanniae recen, 4-8 parts of red ginseng and 2-4 parts of poria cocos.

Further, the traditional Chinese medicine composition also comprises the following raw materials in parts by weight: 1-2 parts of cortex lycii radicis, 1-2 parts of radix ophiopogonis and 1-2 parts of radix asparagi.

Further, the traditional Chinese medicine composition is prepared from the following raw materials in parts by mass: 14-18 parts of radix rehmanniae recen, 2-4 parts of poria cocos, 1-2 parts of cortex lycii radicis, 1-2 parts of radix asparagi, 1-2 parts of radix ophiopogonis and 4-8 parts of red ginseng.

Still further, preferably, the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 16 parts of radix rehmanniae recen, 6 parts of red ginseng, 3 parts of tuckahoe, 1 part of radix ophiopogonis, 1 part of radix asparagi and 1 part of cortex lycii radicis.

The raw materials of the traditional Chinese medicine composition are decocted and extracted by water, and pharmaceutically acceptable auxiliary materials are added to prepare a pharmaceutical preparation of one of the following: tablets, granules, capsules, oral liquids or soft extracts; can also be used as active ingredient for other foods or medicines.

The traditional Chinese medicine composition or the pharmaceutical preparation provided by the invention is used for preparing a medicine for reducing the body fat rate. The dosage of the traditional Chinese medicine composition can be adjusted according to the dosage form characteristics, the age and the physiological state of a patient, the effective dosage of the medicine is 3.0-9.0 g/day in terms of crude drug, generally 30 days is taken as a treatment course, and the medicine can be continuously taken for 2-3 treatment courses.

The invention has the following beneficial effects: the traditional Chinese medicine composition and the preparation containing the composition can reduce the fat percentage of obese people, improve body composition preparation, increase the ratio of brown fat/white fat, and have positive influence on glycolipid metabolic disturbance accompanied by obesity.

Compared with the existing weight-losing medicines and health-care products, the composition has obvious advantages. Firstly, the food is safer, does not affect normal diet, and avoids malnutrition; the burden of the liver and the kidney can not be increased, and the function of the intestinal tract can not be damaged. Secondly, the distribution of adipose tissues in the body is adjusted, the proportion of brown adipose tissues is increased, the proportion of white adipose tissues is reduced, the proportion of skeletal muscles is increased, and weight rebound after weight loss is avoided.

Fourth, detailed description of the invention

The invention will be further described with reference to specific examples, but the scope of the invention is not limited thereto:

example 1

The formula is as follows: 165g of dried rehmannia root, 30g of red ginseng and 33g of tuckahoe.

The preparation method comprises the following steps: taking radix rehmanniae recen, radix asparagi and poria cocos according to the formula amount, adding water with the mass being 10 times of that of the radix rehmanniae recen, the radix asparagi and the poria cocos, decocting for 3 times, decocting for 2 hours for the first time, decocting for 1 hour for the second time and the third time respectively, combining the decoctions, filtering, concentrating the filtrate until the relative density is 1.30-1.40 (60 ℃), obtaining about 250g of extract, adding 200g of honey, and mixing uniformly to prepare the soft extract.

Example 2

The formula is as follows: 409g of dried rehmannia root, 150g of red ginseng and 77g of tuckahoe.

Taking decoction pieces in the formula amount, adding water with the mass being 10 times of that of the decoction pieces, decocting for 3 times, decocting for 2 hours for the first time, decocting for 1 hour for the second time and the third time respectively, combining the decoctions, filtering, concentrating the filtrate to obtain an extract with the relative density of 1.25-1.30 (60 ℃), adding a proper amount of dextrin, granulating, drying, and preparing into granules.

Example 3

The formula is as follows: 150g of radix rehmanniae recen, 55g of red ginseng, 33g of poria cocos, 9g of radix ophiopogonis, 9g of radix asparagi and 9g of cortex lycii radicis.

Taking radix rehmanniae, radix ophiopogonis, radix asparagi, cortex lycii radicis and poria cocos according to the formula amount, adding 8 times of water by mass, decocting for 3 times, decocting for 2 hours for the first time, decocting for 1 hour for the second time and the third time respectively, combining decoctions, filtering, concentrating the filtrate to the relative density of 1.30-1.40 (60 ℃), and obtaining the extract. Pulverizing Ginseng radix Rubri into fine powder, mixing with the extract and appropriate amount of starch, drying, granulating, tabletting, coating, and making into tablet. Or granulating, filling into capsule, and making into capsule.

Example 4

The formula is as follows: 150g of radix rehmanniae recen, 60g of red ginseng, 30g of poria cocos, 10g of radix ophiopogonis, 10g of radix asparagi and 8g of cortex lycii radicis.

The red ginseng slice with the formula amount is distilled by water vapor, and 20ml of aromatic water is collected for standby. Adding the radix rehmanniae, the radix astragali, the poria cocos and the radix asparagi into the distilled red ginseng slices according to the formula, adding 10 times of water by mass, decocting for 3 times, decocting for 2 hours for the first time, decocting for 1 hour for the second time and the third time respectively, mixing the decoctions, filtering, concentrating the filtrate until the relative density is 1.10-1.18 (80 ℃), adding ethanol into the obtained extract until the volume concentration of the ethanol is 60%, precipitating, and concentrating the supernatant to remove the ethanol. Adding sweetener, pH regulator and antiseptic into the extract, filtering, bottling, sterilizing, and making into oral liquid.

Example 5: effect of the composition on body weight and fat distribution of naturally aging mice

1. Test method

C57BL/6 mice, male, 6-8 weeks old, purchased from Shanghai Si Laike laboratory animals, Inc., 18 months old, from Experimental animals technologies, Inc., of Wei Tongli, Beijing. The experimental animals are raised in the cleaning grade of the experimental animal center of Zhejiang university.

Grouping: the experimental mice were divided into 4 groups of 6 weeks old (light year control, n ═ 6), 6 weeks old/composition group (light year administration, n ═ 6), 18 months old (aged control, n ═ 5), 18 months old/composition group (aged administration, n ═ 5), respectively. Administration: an extract was prepared as in example 1. The dose was 0.45g/kg/d (corresponding to 0.9g crude drug) and the administration was continued for 8 weeks. After 8 weeks of dosing, mice were sacrificed by cervical dislocation. White adipose tissues at the groin and brown adipose tissues at the scapula of each group of mice were taken and weighed. The brown/white fat ratio and the brown fat percentage (brown fat/body weight) were calculated. Mean ± standard deviation of experimental data, data processing and plotting using GraphPad Prime software, and comparisons between groups using one-way ANOVA (one-way ANOVA), p <0.05 was statistically significant.

2. Results of the experiment

2.1 mouse weight changes

Each group of mice was administered for 8 weeks continuously, and body weights were weighed weekly, and the results are shown in Table 1. The weight of mice in the young group is generally in an upward trend, and the weight of mice in the old group is in a downward trend. The composition has slight weight reducing effect on animals.

TABLE 1 weight change (unit g) for each group of mice

2.2 mouse fat distribution Change

The changes in the fat distribution of the mice in each group are summarized in Table 2. Compared with the mice of 6-8 weeks, the mass ratio (%) of white fat and the mass ratio of brown fat/white fat of the mice of 18 months were significantly increased and decreased (p < 0.05). The composition increased the content of brown fat and the brown fat mass ratio (brown fat/body weight, g/g%) in young and old mice compared to control mice. The difference in the young group was statistically significant (p < 0.01); the individual differences among the aged groups are large, and although the population tends to rise, the statistical differences are not existed.

TABLE 2 fat distribution Change in groups of mice

Note: p <0.05, p <0.01, compared to light year controls;^#p＜0.05,^##p is less than 0.01, compared with the aged control group.

Example 6: effect of compositions on body fat percentage of obesity models

1. Experimental methods

6-8 week-old SPF grade C57BL/6 male mice 30, weighing 18-20g, were purchased from Shanghai Rui Temous Biotech, Inc., license number SCXK (Shanghai) 2016-0001.

Mice were acclimatized one week later and divided into 4 groups, namely a conventional diet group (ND or a, n ═ 5), a conventional diet composition group (ND-K or C, n ═ 5), a high-fat diet group (HFD or a, n ═ 10), and a high-fat diet composition group (HFD-K or C, n ═ 10). Granules of the composition (dry granules before tabletting) were prepared in the manner of example 3. The conventional feed is provided by the Experimental animal center in Zhejiang province, and the execution standard GB 14924.1-2001; high fat diet (D12492, Open Source Diets available from Beijing Special technology Ltd.) had an energy ration of 60% fat, 20% protein, and 20% carbohydrate.

The conventional diet composition group (ND-K) and the high fat diet composition group (HFD-K) were gavaged with 0.45g/kg/d of the aqueous composition solution daily, and the conventional diet group (ND) and the high fat diet group (HFD) were gavaged with the same amount of distilled water daily. Animals were free to ingest water and mice weight was recorded weekly for 1 meal. After 12 weeks of intervention, the weight of the mice is weighed after fasting for 4h, blood is taken from the eyeballs, and the blood serum is separated and refrigerated. Tissues such as visceral fat (epididymis, perirenal and mesentery), subcutaneous fat (limbs), brown fat (scapular region), muscles (left hind limb gastrocnemius and soleus) are weighed. Mean ± standard deviation of experimental data, data processing and mapping using GraphPad Prime software, with t-test or rank-sum test for comparisons between groups, with p <0.05 being statistically significant.

2. Results of the experiment

2.1 mouse weight changes

The weight changes of the mice in each group are summarized in Table 3. The obesity model is successfully modeled on the 17 th day, the body weight of the mice in the HFD group is higher than 2 standard deviations of the mice in the ND group, the difference is statistically significant, and the body weight of the HFD group is obviously higher than that of the mice in the ND group, and the model is stable. At the end of the experiment, the HFD group was significantly heavier than the ND group; compared with the HFD group, the mice in the HFD-K group have reduced body weight and have significant difference.

TABLE 3 weekly body weight changes (g) for each group of mice

Note: p <0.05, p <0.01, compared to normal feed group; # p <0.05, # p <0.01 compared to the high-fat diet group.

2.2 changes in body composition of mice

The results of the weight of the mouse fat and skeletal muscle and the weight ratio of each are summarized in Table 4. Compared with the ND group, the absolute values of visceral fat, subcutaneous fat and brown fat of the mice in the HFD group are all obviously increased. The percentage of each adipose tissue in the body weight is increased to different degrees, and subcutaneous fat > visceral fat > brown fat are sequentially changed from high to low. The composition can significantly reduce the percentage of visceral and subcutaneous fat in obese mice without reducing healthy brown adipose tissue.

The absolute weight of skeletal muscle of mice in the HFD group was not significantly changed, but the percentage of body weight was significantly decreased, i.e., the relative amount was significantly decreased, as compared with the ND group. The composition can significantly improve the percentage of skeletal muscle of obese mice.

TABLE 4 variation of body composition of mice in each group

2.3 Biochemical index Change in blood of mouse

The results of biochemical blood tests of the mice in each group are summarized in Table 5. Compared with the ND group, the blood sugar of the HFD group is obviously increased. The composition can obviously reduce the blood sugar content of obese mice, and has no adverse effect on liver and kidney functions.

TABLE 5 Biochemical index changes in blood of mice in each group

Claims

1. The application of a traditional Chinese medicine composition in preparing a medicine for adjusting the fat proportion is characterized in that: the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 14-18 parts of radix rehmanniae recen, 4-8 parts of red ginseng and 2-4 parts of poria cocos.

2. The use of claim 1, wherein: the traditional Chinese medicine composition is prepared from the raw materials in a water extraction mode.

3. An application of a traditional Chinese medicine preparation in preparing a medicine for adjusting fat proportion is characterized in that: the traditional Chinese medicine preparation consists of a traditional Chinese medicine composition and pharmaceutically acceptable auxiliary materials; the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 14-18 parts of radix rehmanniae recen, 4-8 parts of red ginseng and 2-4 parts of poria cocos.

4. Use according to claim 3, characterized in that: the preparation is tablets, granules, capsules, oral liquid or soft extract.

5. The application of a traditional Chinese medicine composition in preparing a medicine for adjusting the fat proportion is characterized in that: the traditional Chinese medicine composition is prepared from the following raw materials in parts by mass: 14-18 parts of radix rehmanniae recen, 2-4 parts of poria cocos, 1-2 parts of cortex lycii radicis, 1-2 parts of radix asparagi, 1-2 parts of radix ophiopogonis and 4-8 parts of red ginseng.

6. The use of claim 5, wherein: the traditional Chinese medicine composition is prepared from the following raw materials in parts by mass: 16 parts of radix rehmanniae recen, 6 parts of red ginseng, 3 parts of tuckahoe, 1 part of radix ophiopogonis, 1 part of radix asparagi and 1 part of cortex lycii radicis.

7. The use of claim 5, wherein: the traditional Chinese medicine composition is prepared from the raw materials in a water extraction mode.

8. An application of a traditional Chinese medicine preparation in preparing a medicine for adjusting fat proportion is characterized in that: the traditional Chinese medicine preparation consists of a traditional Chinese medicine composition and pharmaceutically acceptable auxiliary materials; the traditional Chinese medicine composition is prepared from the following raw materials in parts by mass: 14-18 parts of radix rehmanniae recen, 2-4 parts of poria cocos, 1-2 parts of cortex lycii radicis, 1-2 parts of radix asparagi, 1-2 parts of radix ophiopogonis and 4-8 parts of red ginseng.

9. The use of claim 8, wherein: the traditional Chinese medicine composition is prepared from the following raw materials in parts by mass: 16 parts of radix rehmanniae recen, 6 parts of red ginseng, 3 parts of tuckahoe, 1 part of radix ophiopogonis, 1 part of radix asparagi and 1 part of cortex lycii radicis.

10. The use of claim 8, wherein: the preparation is tablets, granules, capsules, oral liquid or soft extract.