CN108795818A - A kind of multifunctional bio bacterial manure - Google Patents
A kind of multifunctional bio bacterial manure Download PDFInfo
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- CN108795818A CN108795818A CN201810708386.XA CN201810708386A CN108795818A CN 108795818 A CN108795818 A CN 108795818A CN 201810708386 A CN201810708386 A CN 201810708386A CN 108795818 A CN108795818 A CN 108795818A
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- bacterium solution
- bacterial manure
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- multifunctional bio
- strain
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The present invention provides a kind of multifunctional bio bacterial manure, including：First bacterium solution, for adsorbing the nitrate nitrogen in soil；With the second bacterium solution, for preventing root-knot nematode, the strain of the first bacterium solution is denitrifying bacteria and sporotrichum thermophile, and the strain of the second bacterium solution is：Bacillus subtilis and Paecilomyces lilacinus.When first bacterium solution and the second bacterium solution are co-administered, the effect of prevention root-knot nematode is much better than that the second bacterium solution is administered alone, and the synergistic effect between microorganism causes the effect for the bio-bacterial manure being co-administered to be promoted there are one great.
Description
Technical field
The present invention relates to a kind of bio-bacterial manure, more particularly to a kind of multifunctional bio bacterial manure.
Background technology
Bio-bacterial manure, also known as microbial manure are to cause crop to obtain specific fertilizer effect with the vital movement of microorganism
A kind of product, be in agricultural production use fertilizer one kind.Mainly by a large amount of beneficial microorganisms contained in bio-bacterial manure
Vital movement is completed.Only in the case that these beneficial microbes are in vigorous breeding and metabolism, substance conversion
It could constantly be formed with beneficial metabolic product.Therefore, whether beneficial microorganism type, vital movement are vigorous in microbial manure
It is the basis of its validity, is by the form of the essential elements such as nitrogen, phosphorus, potassium and based on how many rather than other fertilizer.
Currently, whole year chemical fertilizer total dosage in China's, up to 1.4 hundred million tons, equivalent purity nitrogen nutrient is up to 42,400,000 tons.Due to chemical fertilizer
For a long time, a large amount of to use, especially science, unreasonable blindness do not use, and cause the practical efficiency of chemical fertilizer very low.Loss
Nitrogenous fertilizer is increasingly severe to the pollution of air and underground water.In recent years, cyanobacteria is broken out in Taihu Lake, Chaohu and Dian Chi in succession, serious to endanger
And the safe drinking water and aquaculture of the periphery masses.Therefore, it is badly in need of providing a kind of bio-bacterial manure that can weaken nitrogen pollution, it is right
Safe drinking water and aquaculture are protected.
Root-knot nematode (Meloidogyne) is a kind of omnivorousness plant pathogeny line insect of height specialized form.Root-knot nematode master
Cause harm the roots of various vegetables, shows as lateral root and fibrous root more normally increases, and form spherical or circle on the fibrous root of young root
The white root nodule that taper differs in size, some are in beads shape.Killed strain overground part growth is short and small, slow, leaf color is abnormal, as a result
It is few, low output, or even plant is caused to do sth. in advance death.Therefore, it is very important for crops to the prevention of root-knot nematode.
Root-knot nematode will be prevented in the prior art not yet and weakens the bio-bacterial manure that nitrogen pollution is combined, and this
It is very important, because studies show that, the outburst rate of the usual root-knot nematode in location of nitrogen pollution can be than remaining location
It is high.
Invention content
In view of this, the main purpose of the present invention is to provide a kind of bio-bacterial manure, one side can prevent root knot line
On the other hand worm can weaken nitrogen pollution.
In order to achieve the above objectives, the technical proposal of the invention is realized in this way：The present invention provides a kind of multifunctional bio
Bacterial manure, which is characterized in that the multifunctional bio bacterial manure includes：First bacterium solution, for adsorbing the nitrate nitrogen in soil；With second
Bacterium solution, for preventing root-knot nematode.Further, the strain of first bacterium solution is denitrifying bacteria and sporotrichum thermophile.Into
One step, the strain of second bacterium solution is：Bacillus subtilis and Paecilomyces lilacinus.
Denitrifying bacteria is purchased from North Sea Qun Lin bioengineering Co., Ltd；Sporotrichum thermophile is purchased from Shanghai name strength biotechnology
Co., Ltd；Bacillus subtilis is purchased from North Sea Qun Lin bioengineering Co., Ltd；Paecilomyces lilacinus is purchased from Shandong Changtai biology
Science and Technology Ltd..
In one embodiment, the ratio of described two bacterium solutions is 1:2.In another embodiment, more work(
Energy bacterial manure can also include carrier.Other than bacteria-removing liquid, it is this to be used to prepare the required other materials of bio-bacterial manure and technology
Conventional material known to field technology personnel and technology.
Inventor makes us the discovery expected, and when the first bacterium solution and the second bacterium solution are co-administered, effect is much better than independent
Using the second bacterium solution, the synergistic effect between microorganism causes the effect for the bio-bacterial manure being co-administered there are one greatly carrying
It rises.
Specific implementation mode
The preparation of embodiment one, the first bacterium solution
The preparation method of first bacterium solution includes the following steps：
1. the preparation of denitrifying bacteria bacterium solution：
A. inclined-plane culture：Denitrifying bacteria original strain is aseptically inoculated on slant medium, at 37 DEG C
Under the conditions of cultivate 36 hours；
B. shaking table culture：The strain that step a is cultivated aseptically is inoculated in seed culture medium, in pH7.0,37
Under the conditions of DEG C, 200r/min shaking table cultures 16 hours；
C. fermentation tank culture：The strain that step b is cultivated aseptically is inoculated in liquid fermentation medium,
PH7.5, tank pressure 0.05Mpa, under the conditions of 65 DEG C, after culture 45 hours, viable count is more than 2.0 × 108Cfu/mL, 90% thalline at
For gemma tank at present, zymotic fluid is obtained.
Wherein, the formula of the slant medium used in step a is as follows：Sodium nitrate 0.15%, sodium acetate 0.1%, phosphoric acid
Potassium dihydrogen 0.1%, glucose 0.15%, magnesium sulfate 0.01%, aspartic acid 0.05%, ferric trichloride 0.005%, yeast extract
0.015%, agar 2.0%, surplus is water, pH value 7.0；The culture medium prescription used in step b and c is as follows：Sodium nitrate
0.2%, sodium acetate 0.1%, potassium dihydrogen phosphate 0.1%, glucose 0.15%, magnesium sulfate 0.02%, aspartic acid 0.05%, three
Iron chloride 0.01%, yeast extract 0.015%, surplus are water, pH value 7.2.
2. the preparation of sporotrichum thermophile bacterium solution：
A. inclined-plane culture：Sporotrichum thermophile original strain is aseptically inoculated on slant medium, at 37 DEG C
Under the conditions of cultivate 36 hours；
B. shaking table culture：The strain that step a is cultivated aseptically is inoculated in seed culture medium, in pH7.0,37
Under the conditions of DEG C, 150r/min shaking table cultures 16 hours；
C. fermentation tank culture：The strain that step b is cultivated aseptically is inoculated in liquid fermentation medium,
PH7.2, tank pressure 0.05Mpa, under the conditions of 37 DEG C, after culture 48 hours, viable count is more than 1.0 × 109Cfu/mL obtains zymotic fluid.
Wherein, the formula of the slant medium used in step a is as follows：Rice bran：Wheat bran：Corn flour：Dregs of beans：Water-retaining property tree
Glue=1:1.5:1.5:2.5:0.1 ratio mixing；The culture medium prescription used in step b and c is PDA culture medium and contains
The rare earth rush culture medium of lanthanum, cerium, its ratio be 1:0.0003.Denitrifying bacteria bacterium solution and sporotrichum thermophile bacterium solution are mixed to get
One bacterium solution.
The preparation of embodiment two, the second bacterium solution
1. the preparation of bacillus subtilis bacterium solution：
A. inclined-plane culture：Bacillus subtilis original strain is aseptically inoculated on slant medium, 37
It is cultivated 42 hours under the conditions of DEG C；
B. shaking table culture：The strain that step a is cultivated aseptically is inoculated in seed culture medium, in pH7.0,37
Under the conditions of DEG C, 150r/min shaking table cultures 18 hours；
C. fermentation tank culture：The strain that step b is cultivated aseptically is inoculated in liquid fermentation medium,
PH7.5, tank pressure 0.05Mpa, under the conditions of 37 DEG C, after culture 56 hours, viable count is more than 5.0 × 108Cfu/mL, 80% thalline at
For gemma tank at present, zymotic fluid is obtained.
Wherein, the formula of the slant medium used in step a is as follows：Glucose 15g, peptone 5g, yeast extract 5g, fine jade
Fat 15g and water 1000mL；The seed culture based formulas used in step b is as follows：Glucose 10g, beef extract 5g, yeast powder 5g,
Starch 10g, beancake powder 5g, KH2PO4 0.5g, MgSO4 0.2g and water 1000mL；The liquid fermentation medium used in step c
Formula is as follows：Corn flour 26kg, beancake powder 16kg, ammonium sulfate 4kg, glucose 8kg, yeast powder 2.5kg, peptone 1.7kg and
Antifoaming agent 50mL, adds water to 600L.
2. the preparation of Paecilomyces lilacinus bacterium solution：
A. Paecilomyces lilacinus original strain is aseptically inoculated on slant medium, is cultivated under the conditions of 28 DEG C
72 hours；
B. shaking table culture：The strain that step e is cultivated aseptically is inoculated in seed culture medium, in pH6.0,26
Under the conditions of DEG C, 160r/min shaking table cultures 24 hours；
C. fermentation tank culture：The step f strains cultivated aseptically are inoculated in liquid fermentation medium respectively,
PH6.0, tank pressure 0.05Mpa, under the conditions of 26 DEG C, fermentation is terminated after 72 hours in culture, solid fermentation is carried out and produces spore；
D. solid fermentation produces spore：Mycelium of the step c after fermentation tank culture is inoculated on solid fermentation culture medium,
Culture 168-180 hours stops fermentation when Paecilomyces lilacinus 90% produces spore；It is resuspended again with liquid fermentation medium and obtains bacterium
Liquid.
Wherein, the formula of the slant medium used in step a is as follows：Glucose 20g, murphy juice 200g, agar 20g and
Water 1000mL；The seed culture based formulas used in step b is as follows：White sugar 20g, peptone 5g, potassium dihydrogen phosphate 0.2g, sulfuric acid
Magnesium 0.2g, sodium chloride 2g and water 1000mL；The liquid fermentation medium formula used in step c is as follows：Starch 12kg, corn flour
3kg, beancake powder 1.2kg, white sugar 12kg, peptone 3kg, magnesium sulfate 0.12kg, sodium chloride 12kg, antifoaming agent 50mL, add water to
600L；Solid fermentation culture medium prescription in step d is as follows：Wheat bran, corn flour and beancake powder press 7:2:1 weight ratio mixing composition
Solid material in water is added to get solid fermentation culture medium, the weight ratio of solid material and water is 1:0.6.Bacillus subtilis bacterium
Liquid and the mixing of Paecilomyces lilacinus bacterium solution, obtain the second bacterium solution.
The field trial of embodiment 3, multifunctional bio bacterial manure
According to experimental design, it is divided into experimental group and control group.Experimental group is to add being suitble to for the first bacterium solution and the second bacterium solution
Fertilizer, the conventional selection that remaining fertilizer essential component is well known to those skilled in the art.Control group is the second bacterium solution, remaining fertilizer
The conventional selection that material essential component is well known to those skilled in the art.To ensure single factor test and avoiding error, the second bacterium is removed
Remaining ingredient other than liquid is consistent, and when field operates, and also takes the various operations for avoiding error.
With reference to the field in microbial manure field experiment technical regulation and fertilizer efficiency evaluation guide (NY/T 1536-2007)
Effect experiment design requirement.Experiment is divided into 3 groups, plants tomato, every group of cultivated area 20m2, every group is done 3 repetitions and tested.Plantation
Density is 3000 plants/acre, and insect pest preventing and controlling etc. are managed by Production of Large Fields requirement.
Experimental group A：Using the bio-bacterial manure comprising the first bacterium solution and the second bacterium solution, the ratio of the first bacterium solution and the second bacterium solution
It is 1:2, dosage 80kg per acre, remaining ingredient is conventional ingredient well known to those skilled in the art, such as (melon and fruit class is special for base manure
80kg/ mus of organic fertilizer, 12kg/ mus of ammonium bicarbonate, 30kg/ mus of calcium superphosphate, 17kg/ mus of potassium acid sulfate) mixing does base manure application；
Experimental group B：Using the bio-bacterial manure for including the second bacterium solution, dosage 80kg, remaining ingredient are art technology per acre
Conventional ingredient known to personnel, such as base manure (80kg/ mus of melon and fruit class fertilizer special for organic, 12kg/ mus of ammonium bicarbonate, calcium superphosphate 30kg/
Mu, 17kg/ mus of potassium acid sulfate) mixing does base manure application；
Experimental group C：For common fertilizer.
Take 10 plants of progress root knot condition surveys at random per cell when tomato is transplanted 60 days.Disease index, which calculates, uses 0~4
The grade scale of grade carries out severity Scaling, counts disease index, and calculate preventive effect.With reference to the grade scale of Xiao Yannong etc.：0 grade,
Without root knot；1 grade, there is the root system of root knot to account for the 1%~24% of full root system；2 grades, have the root system of root knot account for full root system 25%~
49%；3 grades, there is the root system of root knot to account for the 50%~74% of full root system；4 grades, have the root system of root knot account for full root system 75%~
100%.Control effect=[(disease index after blank control disease index-fertilizer treatment)/blank control disease index] ×
100%.The results are shown in Table 1 for it.In addition the case where specifically having investigated the tomato various sick state of an illness, the results are shown in Table 2.
Influence of the 1. 3 kinds of processing of table to root-knot nematode state of an illness index, yield
| Processing | Disease index | Control effect (%) | Per mu yield (Kg) | Rate of growth (%) |
| Experimental group A | 2.1 | 89.3% | 5083 | 23.73% |
| Experimental group B | 6.2 | 72.1% | 4763 | 15.94% |
| Experimental group C | 18.5 | 4108 |
As can be seen from the table, it is not added with the disease index highest of the experimental group C of bio-bacterial manure, per mu yield is minimum.
And it is added to the experimental group A of the first bacterium solution and the second bacterium solution obviously the effect in terms of control effect and rate of growth is all much better than to test
Group B.The experimental group A for demonstrating the first bacterium solution of addition and the second bacterium solution achieves good effect.
2 three kinds of table handles the influence that tomato disease occurs
The foregoing is only a preferred embodiment of the present invention, and the guarantor for being not intended to limit the present invention encloses.
Claims (6)
1. a kind of multifunctional bio bacterial manure, which is characterized in that the multifunctional bio bacterial manure includes：
First bacterium solution, for adsorbing the nitrate nitrogen in soil；With
Second bacterium solution, for preventing root-knot nematode.
2. multifunctional bio bacterial manure according to claim 1, which is characterized in that the strain of first bacterium solution is denitrification
Bacterium and sporotrichum thermophile.
3. multifunctional bio bacterial manure according to claim 2, which is characterized in that the strain of second bacterium solution is：Withered grass
Bacillus and Paecilomyces lilacinus.
4. the multifunctional bio bacterial manure according to claim 1-4, which is characterized in that the ratio of described two bacterium solutions is 1:2.
5. the multifunctional bio bacterial manure according to claim 1-4, which is characterized in that the multi-functional bacterial manure can also include
Carrier.
6. the multifunctional bio bacterial manure described in claim 1-5 is used to prevent the application of root-knot nematode.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1616648A (en) * | 2004-04-14 | 2005-05-18 | 北京天地新生力生物技术有限公司 | Fermentation and preparation for composite bacterial prepn and organic bacterial manure for preventing and controlling soil plant diseases and insect pests |
| CN101507409A (en) * | 2009-03-30 | 2009-08-19 | 王其传 | Disease-resistant, wireworm-resistant seedling raising ground substance and production method thereof |
| CN101861880A (en) * | 2010-06-24 | 2010-10-20 | 云南大学 | Microbial agent and application thereof |
| CN102154159A (en) * | 2010-12-29 | 2011-08-17 | 南京农业大学 | Antagonistic bacteria for preventing and controlling tomato root-knot nematodes and microorganism organic fertilizer produced by same |
| CN107759325A (en) * | 2017-11-03 | 2018-03-06 | 河北益微生物技术有限公司 | A kind of multifunctional biological fertilizer |
-
2018
- 2018-07-02 CN CN201810708386.XA patent/CN108795818A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1616648A (en) * | 2004-04-14 | 2005-05-18 | 北京天地新生力生物技术有限公司 | Fermentation and preparation for composite bacterial prepn and organic bacterial manure for preventing and controlling soil plant diseases and insect pests |
| CN101507409A (en) * | 2009-03-30 | 2009-08-19 | 王其传 | Disease-resistant, wireworm-resistant seedling raising ground substance and production method thereof |
| CN101861880A (en) * | 2010-06-24 | 2010-10-20 | 云南大学 | Microbial agent and application thereof |
| CN102154159A (en) * | 2010-12-29 | 2011-08-17 | 南京农业大学 | Antagonistic bacteria for preventing and controlling tomato root-knot nematodes and microorganism organic fertilizer produced by same |
| CN107759325A (en) * | 2017-11-03 | 2018-03-06 | 河北益微生物技术有限公司 | A kind of multifunctional biological fertilizer |
Non-Patent Citations (2)
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| 杨军玉主编: "《菜病虫害防治彩色图鉴》", 29 February 2016 * |
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