CN108783149A - A kind of production method of curcumin-ovalbumin compound anti-oxidation protein beverage - Google Patents

A kind of production method of curcumin-ovalbumin compound anti-oxidation protein beverage Download PDF

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Publication number
CN108783149A
CN108783149A CN201810474601.4A CN201810474601A CN108783149A CN 108783149 A CN108783149 A CN 108783149A CN 201810474601 A CN201810474601 A CN 201810474601A CN 108783149 A CN108783149 A CN 108783149A
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ovalbumin
curcumin
solution
production method
protein beverage
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CN201810474601.4A
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Inventor
王鹏
徐幸莲
吴长玲
周光宏
杜菲菲
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Nanjing Agricultural University
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Nanjing Agricultural University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/58Colouring agents
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

The present invention discloses a kind of production method of curcumin-ovalbumin compound anti-oxidation protein beverage, includes the following steps:(1) ovalbumin powder be hydrated, obtain concentration 4-8mg/mL ovalbumin solution;(2) curcumin and ovalbumin are pressed 1:Volume ratio mixed preparing curcumin-ovalbumin composite solution of 2-6.Using ovalbumin as carrier, embed curcumin, improve the bioavailability of fat-soluble polyphenol curcumin, form stable curcumin-ovalbumin composite solution, the production method for providing a kind of functional beverage containing Abundant protein source, to meet consumer to health, personalization, the pursuit of functionalization beverage.

Description

A kind of production method of curcumin-ovalbumin compound anti-oxidation protein beverage
Technical field
The invention belongs to protein beverage technical fields, and in particular to a kind of curcumin-ovalbumin compound anti-oxidation albumen The production method of beverage.
Background technology
With the development of the social economy, consumer persistently drops to the preference of high sugar content, high heat beverage, and it is healthy Change, personalized, functionalization beverage are the mainstream demands of future customer, therefore protein beverage will be with larger market potential.
Currently, protein beverage focuses mostly in vegetable protein beverage, such as soya-bean milk, coconut milk, almond milk class beverage, but egg white Protein beverage is less, and ovalbumin is a kind of protein extracted from egg, and, absorption high, easy to digest with nutritive value contains There is the features such as whole essential amino acids and a variety of active ingredients, is generally acknowledged one of human body protein replenishers.
Curcumin is a kind of fat-soluble polyphenol extracted from turmeric, has stronger physiology and pharmacological activity.Curcumin With anti-inflammatory, anti-oxidant, reducing blood lipid, inhibit type II diabetes complication, the inhibition biology such as thrombus and myocardial infarction, anticancer living Property.But it is insoluble under acid condition since curcumin is not soluble in water, it is unstable under neutral and alkaline condition, and greatly reduce Its absorptivity, this greatly limits the utilization of curcumin in the food industry.
Described in patent CN107897915A it is a kind of load curcumin lotus seed protein-pectin complex emulsions and its preparation Method needs to dissolve curcumin by oil phase, improves stability with multi-layer phase, process is complex, patent 201310121024.8 disclose a kind of health drink and preparation method thereof of curcumin and sea-buckthorn composition, by introducing in the beverage The additional emulsifier such as decentralized medium PVP K30 and Tween 80, to ensure the stable dispersion of curcumin in the beverage; Patent 201310036669.1 discloses a kind of edible oil constituent and its preparation method containing curcumin chemical compounds, utilizes edible oil The curcumin composition that organic solvent prepares stable state is substituted, but in this, as beverage raw material, the mouthfeel that beverage can be brought poor And smell.
Therefore in view of the above problems, according to the actual needs of factorial praluction, the present invention uses simple and effective method, with ovum Albumin is carrier, embeds curcumin, improves the bioavailability of fat-soluble polyphenol curcumin, form stable curcumin-ovum Albumin composite solution provides a kind of making side's method of anti-oxidant Chicken Albumin beverage, with meet consumer to health, Personalized, functionalization beverage pursuit.
Invention content
In response to the problems existing in the prior art, the purpose of the present invention is to provide a kind of compound antioxygens of curcumin-ovalbumin Change the production method of protein beverage.
To achieve the above object, the technical solution adopted by the present invention is:
A kind of production method of curcumin-ovalbumin compound anti-oxidation protein beverage, includes the following steps:(1) egg white Albumen powder be hydrated, obtain concentration 4-8mg/mL ovalbumin solution;(2) curcumin and the ovalbumin solution are pressed 1: Volume ratio mixed preparing curcumin-ovalbumin composite solution of 2-6.
Further, in the step (1) ovalbumin powder be hydrated the specific steps are:Ovalbumin powder is dissolved in steaming In distilled water, mixing time 2-3h.
Further, a concentration of 5mg/mL of the ovalbumin solution.
The curcumin a concentration of 0.2-3.2mg/mL, preferably 0.2mg/mL that the further step (2) is added.
Further, curcumin presses 1 with ovalbumin solution in the step (2):The volume ratio of 3-5 mixes, preferably 1:4.
Further, the step (2) the specific steps are:Using pH shift methods, complete egg white egg will be dissolved White pH value of solution is adjusted to 12, and curcumin is added, and adds proper amount of edible essence to non-stimulated smell, after reacting 20-30min, adjusts Back dissolving liquid is 7 to neutral environment pH, curcumin-ovalbumin composite solution is obtained, using pasteurization, by curcumin-ovum Albumin complex liquid is heated to 68~70 DEG C, and keeps the later rapid coolings of this temperature 30min to 4-5 DEG C, as curcumin-ovum Albumin compound anti-oxidation protein beverage.
The measurement of the combination effect of curcumin-ovalbumin, the specific steps are:Take the curcumin-ovalbumin compound Solution centrifuges, and measures the concentration of curcumin in supernatant, and joint efficiency is determined with the size of embedding rate, i.e. embedding rate=supernatant Curcumin concentration in curcumin concentration/composite solution in liquid measures composite solution inoxidizability using ABTS methods.
Compared with prior art, the beneficial effects of the invention are as follows:
(1) compared with prior art, the present invention using simple effective method, the molten of fat-soluble polyphenol curcumin is improved Xie Du and bioavailability form stable curcumin-ovalbumin compound anti-oxidation protein beverage, and it is single to improve tradition The nutritive value and bioactivity of protein beverage, and assign anti-oxidative beverage good color and luster, with meet consumer to health, Personalized, functionalization beverage pursuit.
(2) inoxidizability can be increased after ovalbumin hydrolysis, improve the antioxygenic property of protein beverage.
Specific implementation mode
Below in conjunction with embodiment, technical scheme of the present invention is clearly and completely described, it is clear that described Embodiment is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field All other embodiment that those of ordinary skill is obtained under the conditions of not making creative work, belongs to protection of the present invention Range.
Embodiment 1:
Present embodiments provide a kind of production method of curcumin-ovalbumin compound anti-oxidation protein beverage, including with Lower step:
(1) ovalbumin powder be hydrated, obtain concentration 4-8mg/mL ovalbumin solution;(2) by curcumin and egg white Albumen presses 1:Volume ratio mixed preparing curcumin-ovalbumin composite solution of 2-6, is as follows,
(1) hydration of ovalbumin:By 300mg, 400mg, 500mg, 600mg, 700mg, 800mg ovalbumin powder point It is not dissolved in 100m L distilled water, in stirring 2h on magnetic stirring apparatus, places 6h, this step is completed under 1-5 DEG C of environment.
(2) preparation of curcumin-ovalbumin composite solution:Using pH shift methods, complete 50mL ovum will be dissolved Albumin soln pH is adjusted to 12, and after keeping 30min, curcumin and ovalbumin solution press 1:0.2mg/mL is added in 4 volume ratios Curcumin, adds proper amount of edible essence to non-stimulated smell, after reacting 30min, then composite solution pH is adjusted to neutrality ring Border pH is 7, obtains curcumin-ovalbumin composite solution, using pasteurization, curcumin-ovalbumin complex liquid is added Heat keeps the later rapid coolings of this temperature 30min to 4-5 DEG C to 68~70 DEG C, as the compound antioxygen of curcumin-ovalbumin Change beverage.
After measured:After pH shifting processing, under different ovalbumin concentration conditions, the embedding effect of ovalbumin As shown in the table, in a concentration of 5mg/mL of ovalbumin, the embedding rate of curcumin-ovalbumin compound is maximum, reachable 61.7%, illustrate that embedding effect is best at this concentration.
Ovalbumin concentration (mg/mL) Embedding rate (%)
3 25.8
4 40.6
5 61.7
6 52.6
7 46.5
8 30.4
Embodiment 2:Investigate influence of the various concentration curcumin to the coating rate of ovalbumin
Influence of the various concentration curcumin to the coating rate of ovalbumin, specific steps are investigated on the basis of embodiment 1 It is as follows:
(1) hydration of ovalbumin:500mg ovalbumin powder is dissolved in 100mL distilled water, on magnetic stirring apparatus 2h is stirred, 6h is placed, this step is completed under 1-5 DEG C of environment.
(2) preparation of curcumin-ovalbumin composite solution:Using pH shift methods, complete 50mL ovum will be dissolved Albumin soln pH is adjusted to 12, and after keeping 30min, curcumin presses 1 with ovalbumin:Various concentration is added in 4 volume ratio Curcumin (0.2,0.4,0.8,1.6,3.2mg/mL), adds proper amount of edible essence to non-stimulated smell, after reacting 30min, It is 7 that composite solution pH, which is adjusted to neutrality environment pH, again, obtains curcumin-ovalbumin composite solution, using pasteurization, Curcumin-ovalbumin complex liquid is heated to 68~70 DEG C, and keeps the later rapid coolings of this temperature 30min to 4-5 DEG C, i.e., For curcumin-ovalbumin compound anti-oxidation beverage.
After measured:After pH shifting processing, under different curcumin concentration conditions, the embedding effect of ovalbumin is such as Shown in following table, in a concentration of 0.2mg/mL of curcumin, the embedding rate of curcumin-ovalbumin compound is maximum, reachable 61.7%, illustrate that embedding effect is best at this concentration.
Curcumin concentration (mg/mL) Embedding rate (%)
0.2 61.7
0.4 50.5
0.8 44.2
1.6 38.4
3.2 18.2
Embodiment 3:
Whether the activity that curcumin and ovalbumin compound rear curcumin are investigated on the basis of Examples 1 and 2 changes.
(1) hydration of ovalbumin:500mg ovalbumin powder is dissolved in 100mL distilled water, on magnetic stirring apparatus 2h is stirred, 6h is placed, this step is completed under 1-5 DEG C of environment.
(2) preparation of curcumin-ovalbumin composite solution:Using pH shift methods, complete 50mL ovum will be dissolved Albumin soln pH is adjusted to 12, and after keeping 30min, curcumin presses 1 with ovalbumin:0.2mg/mL turmerics are added in 4 volume ratios Element, adds proper amount of edible essence to non-stimulated smell, after reacting 30min, then composite solution pH is adjusted to neutrality environment pH It is 7, obtains curcumin-ovalbumin composite solution, using pasteurization, curcumin-ovalbumin complex liquid is heated to 68~70 DEG C, and keep the later rapid coolings of this temperature 30min to 4-5 DEG C, as curcumin-ovalbumin compound anti-oxidation is drunk Material.
After measured:It was found that the ABTS free radical scavenging activities of free curcumin and curcumin-ovalbumin compound are almost It is not different, as a concentration of 80 μ g/mL of curcumin, free radical scavenging activity is 32.20%.Show best in embodiment 1 Under reaction condition (whole pH7, curcumin concentration 0.2mg/mL), by curcumin and ovalbumin it is compound after have no effect on curcumin Activity has almost the same antioxidant effect with individual curcumin.
Embodiment 4
Curcumin-ovalbumin compound anti-oxidation protein beverage is prepared with the same preparation method of above-described embodiment.
(1) hydration of ovalbumin:500mg ovalbumin powder is dissolved in 100mL distilled water, on magnetic stirring apparatus 2h is stirred, 6h is placed, this step is completed under 1-5 DEG C of environment.
(2) preparation of curcumin-ovalbumin composite solution:Using pH shift methods, complete 50mL ovum will be dissolved Albumin soln pH is adjusted to 10,11,12 respectively, and after keeping 30min, curcumin presses 1 with ovalbumin:Ginger is added in 4 volume ratios Flavine (0.2mg/mL), adds proper amount of edible essence to non-stimulated smell, after reacting 30min, then composite solution pH is adjusted To 7, curcumin-egg protein composite solution is obtained, using pasteurization, curcumin-ovalbumin complex liquid is heated to 68 ~70 DEG C, and keep the later rapid coolings of this temperature 30min to 4-5 DEG C, as curcumin-ovalbumin compound anti-oxidation is drunk Material.
After measured:It was found that after pH shift processing, ovalbumin solution pH is different, and embedding rate and its inoxidizability have Certain difference (such as following table), ovalbumin pH be 12 when, embedding effect preferably and inoxidizability highest.
pH Embedding rate ABTS free radical scavenging activities
12 65.4 32.5
11 56.6 27.3
10 36.5 28.9
Embodiment 5:
In the above-described embodiments under conditions of optimal ovalbumin concentration, curcumin concentration, ovalbumin solution pH, adjust The volume ratio of curcumin and ovalbumin solution is respectively 1 in step (2):1,1:2,1:3,1:4,1:5,1:6,1:7, it surveys respectively Determine the embedding rate of curcumin-ovalbumin compound under the conditions of different volumes ratio, as shown in the table,
The volume ratio of curcumin and ovalbumin solution Coating rate (%)
1:1 20.8
1:2 38.6
1:3 50.6
1:4 61.7
1:5 48.5
1:6 42.8
1:7 25.4
In conjunction with table it is found that when the volume ratio of curcumin and ovalbumin solution is 1:4, the coating rate of ovalbumin is most Greatly.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace And modification, the scope of the present invention is defined by the appended.

Claims (6)

1. a kind of production method of curcumin-ovalbumin compound anti-oxidation protein beverage, which is characterized in that including following step Suddenly:(1) ovalbumin powder be hydrated, obtain concentration 4-8mg/mL ovalbumin solution;(2) by curcumin and the egg white egg White solution presses 1:Volume ratio mixed preparing curcumin-ovalbumin composite solution of 2-6.
2. a kind of production method of curcumin-ovalbumin compound anti-oxidation protein beverage as described in claim 1, feature Be, in the step (1) ovalbumin powder be hydrated the specific steps are:Ovalbumin powder is dissolved in distilled water, is stirred Time 2-3h.
3. a kind of production method of curcumin-ovalbumin compound anti-oxidation protein beverage as described in claim 1, feature It is, a concentration of 5mg/mL of ovalbumin solution obtained in the step (1).
4. a kind of production method of curcumin-ovalbumin compound anti-oxidation protein beverage as described in claim 1, feature It is, a concentration of 0.2-3.2mg/mL of curcumin that the step (2) is added.
5. a kind of production method of curcumin-ovalbumin compound anti-oxidation protein beverage as described in claim 1, feature It is, curcumin presses 1 with ovalbumin solution in the step (2):Volume ratio mixed preparing curcumin-ovalbumin of 3-5 Composite solution.
6. a kind of production method of curcumin-ovalbumin compound anti-oxidation protein beverage as described in claim 1, feature Be, the step (2) the specific steps are:Using pH shift methods, complete ovalbumin solution pH will be dissolved and adjusted To 12, curcumin is added, adds proper amount of edible essence and recalls to solution to neutrality after reacting 20-30min to non-stimulated smell Environment pH is 7, obtains curcumin-ovalbumin composite solution, curcumin-ovalbumin complex liquid is heated to 68~70 DEG C, And keep after this temperature 30min rapid cooling to 4-5 DEG C, as curcumin-ovalbumin compound anti-oxidation protein beverage.
CN201810474601.4A 2018-05-17 2018-05-17 A kind of production method of curcumin-ovalbumin compound anti-oxidation protein beverage Pending CN108783149A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110547464A (en) * 2019-09-23 2019-12-10 南京农业大学 Antioxidant emulsion capable of improving bioavailability of curcumin and preparation method and application thereof
CN112868978A (en) * 2021-03-15 2021-06-01 吉林大学 Curcumin nano-emulsion with fresh-keeping effect and preparation method thereof
CN113647607A (en) * 2021-08-06 2021-11-16 广州大学 Mineral-loaded ovalbumin-polyphenol nanoparticles and preparation method and application thereof
CN113693225A (en) * 2021-08-06 2021-11-26 广州大学 Ovalbumin-ferulic acid-polysaccharide compound emulsion as well as preparation method and application thereof

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110547464A (en) * 2019-09-23 2019-12-10 南京农业大学 Antioxidant emulsion capable of improving bioavailability of curcumin and preparation method and application thereof
CN112868978A (en) * 2021-03-15 2021-06-01 吉林大学 Curcumin nano-emulsion with fresh-keeping effect and preparation method thereof
CN113647607A (en) * 2021-08-06 2021-11-16 广州大学 Mineral-loaded ovalbumin-polyphenol nanoparticles and preparation method and application thereof
CN113693225A (en) * 2021-08-06 2021-11-26 广州大学 Ovalbumin-ferulic acid-polysaccharide compound emulsion as well as preparation method and application thereof
CN113693225B (en) * 2021-08-06 2023-12-08 广州大学 Ovalbumin-ferulic acid-polysaccharide compound emulsion and preparation method and application thereof
CN113647607B (en) * 2021-08-06 2023-12-22 广州大学 Mineral-loaded ovalbumin-polyphenol nano-particles and preparation method and application thereof

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