CN108782370B - Method for evaluating influence of object to be tested on physical exercise endurance and special equipment thereof - Google Patents
Method for evaluating influence of object to be tested on physical exercise endurance and special equipment thereof Download PDFInfo
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- CN108782370B CN108782370B CN201810930154.9A CN201810930154A CN108782370B CN 108782370 B CN108782370 B CN 108782370B CN 201810930154 A CN201810930154 A CN 201810930154A CN 108782370 B CN108782370 B CN 108782370B
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- 230000037080 exercise endurance Effects 0.000 title claims abstract description 15
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- 238000012360 testing method Methods 0.000 claims abstract description 70
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 24
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000001301 oxygen Substances 0.000 claims abstract description 24
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 24
- 238000012216 screening Methods 0.000 claims abstract description 21
- 238000002474 experimental method Methods 0.000 claims abstract description 19
- 230000001105 regulatory effect Effects 0.000 claims abstract description 16
- 230000002929 anti-fatigue Effects 0.000 claims abstract description 14
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
- CVSVTCORWBXHQV-UHFFFAOYSA-N creatine Chemical compound NC(=[NH2+])N(C)CC([O-])=O CVSVTCORWBXHQV-UHFFFAOYSA-N 0.000 description 12
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- UCMIRNVEIXFBKS-UHFFFAOYSA-N beta-alanine Chemical compound NCCC(O)=O UCMIRNVEIXFBKS-UHFFFAOYSA-N 0.000 description 8
- -1 di (2-ethyl) hexyl phthalate Chemical compound 0.000 description 8
- 244000042430 Rhodiola rosea Species 0.000 description 7
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K29/00—Other apparatus for animal husbandry
- A01K29/005—Monitoring or measuring activity, e.g. detecting heat or mating
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/042—Introducing gases into the water, e.g. aerators, air pumps
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Marine Sciences & Fisheries (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a method for evaluating influence of an object to be tested (anti-fatigue drugs, anti-fatigue nutrient substances and pollutants) on physical exercise endurance and special equipment thereof. The method comprises the following steps: 1. correcting the flow rate of the water body of the system; 2. screening experimental fish shoal; 3. swimming endurance test; 4. experimental data collection and result analysis. The special equipment comprises a water tank, a water pump, a container for fish swimming, a flow regulating valve, a flowmeter, a nitrogen cylinder, an oxygen cylinder, a refrigerator and a high-speed camera. The method for evaluating the exercise endurance of the organism can be used for early warning and judging of environmental water body abnormality, drug testing, exercise endurance evaluation of different varieties of small-sized fish in different water body environments, evaluation and testing of nutritional supplements and the like. The device can be assembled by itself, the occupied area is small, the parameters such as temperature, water flow rate, dissolved oxygen and the like can be controlled, the operation is simple and convenient, the cost is lower, and the application of related biological experiments is convenient.
Description
Technical Field
The invention relates to a method for evaluating influence of an object to be tested (anti-fatigue drugs, anti-fatigue nutrient substances and pollutants) on physical exercise endurance and special equipment thereof, and mainly relates to the field of biological experiments.
Background
Exercise endurance refers to the ability of an organism to bear the maximum exercise load, and relates to physiological load, exercise stress endurance and the like, which are important indexes for reflecting the health state of the organism. The method is suitable for evaluation, and is a powerful tool for researching the endurance of exogenous factors (such as environmental poison, medicine, nutrition and the like) to body movement. The fish endurance generally refers to the maximum continuous swimming capability of the fish, and common indexes for measuring the maximum continuous swimming capability of the fish are the maximum continuous swimming speed and the continuous swimming time of the fish, the maximum continuous swimming capability of the fish body can reflect the overall movement capability of the fish, and the fish usually swims for a period of time at maximum speed only when encountering natural enemies or counter-current so as to avoid the pursuit of the natural enemies or resist water flow. The maximum continuous swimming capacities of different fish species are different, and the fish can resist the flow velocity which is 3-10 times of the body length, for example, the zebra fish body is about 3 cm long, the maximum countercurrent flow velocity can resist about 30cm/s, and if the maximum continuous swimming flow velocity is exceeded, the fish only fatigues and is pushed away by water flow.
The dissolved oxygen amount, the temperature and different water environments in water have different influences on the continuous swimming ability of fish, and the good or bad influences can be quantified through data detection, so that a method for evaluating the effect of substances on the endurance of the body to movement is formed.
The current commercial measurement of the maximum continuous swimming capability of fish is made by Loligo company alone, and the principle is that in a large water tank, the rotation of a propeller is utilized to produce rapid vortex water flow, and then the fish is put into a swimming channel for countercurrent swimming. To correct the water flow rate, loligo uses laser to irradiate small fluorescent beads in the water, and uses high-speed CCD to capture images for calculation and correction. The whole set of equipment provided by Loligo company has huge volume, the overall market of hardware is about 22 ten thousand RMB, the cost is expensive, and the water flow of the open water body is unstable, which is not beneficial to the stable control of data in the experiment and influences the accuracy of the experimental data.
In addition, through network searching in different ways, the patent of the Yangtze river institute of aquatic science (CN 103202254B) and the patent of the university of three gorges (CN 204047610U) of China are designed for measuring swimming ability of large fishes, the volume is too large, and the maximum flow rate which can be manufactured is too low. Therefore, whether academic or commercial equipment is adopted, the important point to be overcome when the maximum continuous swimming capability of the fish is measured at present is that firstly, whether the equipment can be miniaturized or not, and the volume and the cost of the equipment are reduced. 2. How to correct the water flow velocity and obtain more accurate water linear flow velocity. 3. How to simultaneously adjust the dissolved oxygen and the temperature, and conveniently study the influence of the dissolved oxygen and the temperature on the maximum continuous swimming capability of the fish.
Disclosure of Invention
The invention overcomes the defects of the prior art, provides a method for evaluating the influence of an object to be tested on the exercise endurance of a body and special equipment thereof, and can be used for evaluating and testing the fatigue resistance of medicines, detecting the abnormal environment of a water body, toxicological experiments, evaluating and testing the endurance of different varieties of small-sized fishes, testing the influence of different dissolved oxygen and environmental temperature on the endurance of the fishes, evaluating and testing the using effect of nutritional supplements and the like.
The technical scheme of the invention is as follows:
An apparatus for evaluating endurance of a fish body, comprising a water tank, a water pump and a tubular container for fish to swim therein; the water inlet of the water pump is communicated with the water tank for pumping water from the water tank, the water outlet of the water pump is communicated with the water inlet of the tubular container through a water pipe, and the water outlet of the tubular container is communicated with the water tank for returning water to the water tank.
Preferably, the water pump is a submersible pump immersed in the water tank.
Preferably, the device for evaluating endurance of the fish body further comprises a regulating valve for switching on and off the waterway and regulating the flow, wherein the regulating valve is arranged on a water pipe which is communicated with the water outlet of the water pump and the water inlet of the tubular container.
Preferably, the tubular container is a hollow transparent round tube; the opening at one end of the hollow transparent round tube in the length direction is used as a water inlet, the opening at the other end of the hollow transparent round tube in the length direction is used as a water outlet, and the hollow transparent round tube is provided with openings at two ends and is closed; the diameter of the hollow transparent round tube is 6-10cm, and the length is 30-50cm; the hollow transparent round tube material is preferably an acrylic material.
Preferably, the device for evaluating endurance of the fish body further comprises a flowmeter for detecting flow and flow rate, wherein the flowmeter is arranged on a water pipe for communicating the water outlet of the tubular container with the water tank.
The preferable equipment for evaluating the endurance of the fish body further comprises a nitrogen cylinder and an oxygen cylinder, wherein air outlets of air outlet pipes on the nitrogen cylinder and the oxygen cylinder are immersed in water in the water tank so as to supply oxygen and nitrogen to the water in the water tank.
Preferably, the device for evaluating endurance of the fish body further comprises a high-speed camera, wherein the high-speed camera is arranged above or/and at the side of the tubular container, so as to record the experimental phenomena in the tubular container.
Preferably, the device for evaluating endurance of the fish body further comprises a refrigerator for cooling water in the water tank, wherein the refrigerator is used for cooling water in a fish pond or a fish tank.
The preferable device for evaluating the endurance of the fish body further comprises an interception net for soft interception of the fish, wherein the mesh of the interception net is a honeycomb (regular hexagon) mesh; the intercepting net is arranged at the water inlet and the water outlet of the tubular container respectively, so that the main purpose is to intercept the fatigued fish, and the intercepted fish is not damaged due to the plastic material.
Preferably, the device for evaluating the endurance of the fish body further comprises a temperature sensor for detecting the water temperature of the water in the water tank, and the detection end of the temperature sensor is immersed in the water tank.
The invention also discloses application of the device in evaluating the influence of the anti-fatigue drugs or the anti-fatigue nutrient substances on the exercise endurance of the organism.
The invention also discloses application of the device in evaluating the influence of pollutants on the exercise endurance of a body.
The invention also discloses application of the device in detecting endurance of small-sized fishes of different varieties.
The invention also discloses application of the device in detecting influence of dissolved oxygen and/or ambient temperature on endurance of fish bodies.
A method for evaluating the influence of an object to be tested (anti-fatigue drugs, anti-fatigue nutrient substances and pollutants) on the exercise endurance of an organism by adopting the equipment,
The method comprises the following steps:
Step 1: correcting the flow rate of the water body of the system: and (3) starting the device, adjusting the water flow control valve to start the water pump to generate water flow, and recording and testing a flow value corresponding to the frequency of the variable-frequency motor of the water pump to form a linear comparison graph (shown in figure 2) of the frequency and the water flow of the motor of the water pump. The proper water flow rate is to ensure that the small fish swimming can be kept in the hollow transparent round tube for a certain time, so that the fish cannot advance due to the fact that the flow rate is too large, and meanwhile, the flow rate cannot be too low, otherwise, the experimental time is too long. The mathematical operation formula of the linear flow velocity of the water body is as follows:
υ＝Q/A
wherein: the v is the waterline flow rate, and the unit is cm/s; q is the volume flow of the water pump, and the unit is L/min; a is the cross-sectional area of a cylinder in cm
Step 2: experimental fish swarm screening: before the experiment, putting the shoal of fish into a hollow transparent circular tube filled with water to maintain the anhydrous flow rate so as to adapt the fish to 3-5 minutes, slowly increasing the water flow rate to 1.4-6.8BL/sec (BL/sec is a unit for describing the swimming speed of the fish and means the body length/sec) for 1-3 minutes, slowly increasing the water flow rate to 1.9-7.6BL/sec for 10-15 minutes, and selecting the experimental fish capable of completing the training;
the preferred fish shoal in the step 2 is fish with the body length and the body width respectively smaller than the length and the width of the hollow transparent round tube, such as zebra fish, small catfish, crucian, and the like.
The method comprises the following steps: swimming endurance test:
Preparing or/and collecting water containing the object to be tested, placing the water into an independent experimental cylinder to serve as an experimental group, and setting normal water without the object to be tested as a control group. The number of fish in each group is 7-15. During the experiment, the experimental fishes are respectively placed into the respective experimental cylinders to be treated for 3-5 days, and the fishes in each group are not fed during the treatment period of the to-be-detected object. And (3) placing each group of experimental fish shoals after the treatment into a hollow transparent round tube filled with water respectively for the adaptation training in the step (2).
When the test is started, each group of fishes is respectively placed in the hollow transparent circular tube, the motor is adjusted to the frequency selected in the step 2 in a variable frequency manner, the swimming fatigue time is recorded, the maximum swimming time is recorded to be not more than 20 minutes, and the test time is preferably 7-12 minutes, so that the superiority of rapidness and convenience in the experiment is reflected.
The swimming fatigue is that the hollow transparent round tube keeps swimming in countercurrent until the swimming is pushed by water flow to stop for more than 3s by the interception net.
Preferred test substances in step 3 include, but are not limited to, anti-fatigue drugs, nutritional supplements, pollutants, and the like that have an effect on physical exercise tolerance.
Step 4: experimental data collection and result analysis: the water flow rate and the swimming fatigue time of each group of experimental fish were recorded during the experiment. And carrying out statistical analysis on the experimental group and the control group of the object to be detected by adopting a statistical tool so as to judge the influence of the object to be detected on the swimming performance of the fish body. If P between the two groups is less than 0.05, the object to be tested has a significant effect on the swimming movement capacity of the fish body.
The evaluation method can evaluate the anti-fatigue drugs, thereby being beneficial to screening experiment work of the anti-fatigue drugs, can early warn water body abnormal conditions, can evaluate and test nutritional supplements, can measure the endurance of different kinds of fishes, can evaluate the endurance of the fishes under different dissolved oxygen conditions and temperature conditions, and the like.
The invention has the beneficial effects that: the method and the special equipment for evaluating the influence of the object to be tested on the exercise endurance of the organism can be used for carrying out early warning judgment of the abnormality of the environmental water body, anti-fatigue test of the medicine, exercise endurance evaluation of different varieties of small-sized fish in different water body environments, evaluation test of nutritional supplements and the like. The evaluation method does not need to use the prior high-price measuring equipment, and the detection equipment is nearly ten times lower than the selling price of the conventional market; the special detection equipment in the method can be assembled by itself, is exquisite in design, small in occupied area, simple and convenient to operate and convenient to use; the device comprises the regulating valve, so that the flow and the flow rate of the water body can be accurately regulated, the stable linear flow rate of the water body is maintained, and the stability of a test is ensured; the device can simulate the influence of different dissolved oxygen environments and different temperature environments on the endurance of the fish body, and can detect and collect related data; a closed linear swimming channel is created in a main body test container in the device, and can accurately measure, regulate and record various environmental data relative to the water environment of an open water body or an annular channel, so that the accuracy and the reproducibility of the test are ensured. The evaluation method has the advantages of wide application field, obvious experimental result, convenient operation, short experimental period and good popularization prospect.
Drawings
FIG. 1 is a schematic diagram of a device for evaluating endurance of a fish body;
In the figure: 1 water tank, 2 submerged pump, 3 tubular container, 4 regulating valve, 5 flowmeter, 6 nitrogen cylinder, 7 oxygen cylinder, 8 high-speed camera, 9 refrigerator;
Fig. 2 is: a water pump motor frequency and water flow linear comparison chart;
fig. 3 is: bar graph of the effect of 0.1% ethanol solution on zebra fish endurance.
Fig. 4 is: bar graph of the effect of creatine and beta-alanine solutions on zebra fish endurance.
Fig. 5 is: histogram of comparison of maximum swimming endurance of zebra fish and catfish.
Fig. 6 is: bar graph of the effect of 1ppm ZnCl 2 solution on zebra fish endurance.
Fig. 7 is: bar graph of the effect of 1ppm PbCl 2 solution on zebra fish endurance.
Fig. 8 is: the effect of rhodiola rosea extract on zebra fish endurance influences the histogram of the results.
Fig. 9 is: bar graph of the effect of plasticizer di (2-ethyl) hexyl phthalate on zebra fish endurance.
Fig. 10 is: bar graph of the effect of nanofiller C60 on zebra fish endurance.
Detailed Description
Referring to fig. 1, fig. 1 is a schematic structural diagram of an apparatus for evaluating endurance of a fish body according to an embodiment of the present invention.
The application provides equipment for evaluating endurance of a fish body, which comprises a water tank, a water pump and a tubular container for fish to swim in;
The water inlet of the water pump is communicated with the water tank for pumping water from the water tank, the water outlet of the water pump is communicated with the water inlet of the tubular container through a water pipe, and the water outlet of the tubular container is communicated with the water tank for returning water to the water tank.
In one embodiment of the application, the water pump is a submersible pump submerged in the water in the tank.
In one embodiment of the application, the device for evaluating endurance of the fish body further comprises a regulating valve for switching on and off a waterway and regulating flow, wherein the regulating valve is arranged on a water pipe for communicating a water outlet of the water pump with a water inlet of the tubular container; the device comprises the regulating valve, can accurately regulate the water flow and the flow velocity, maintain the stable linear flow velocity of the water, and ensure the stability of the test.
In one embodiment of the application, the tubular container is a hollow transparent round tube; the opening at one end of the hollow transparent round tube in the length direction is used as a water inlet, the opening at the other end of the hollow transparent round tube in the length direction is used as a water outlet, and the hollow transparent round tube is provided with openings at two ends and is closed; therefore, a closed swimming channel is created in the transparent circular tube, and various environmental data can be accurately measured, regulated and recorded relative to an open water body environment, so that the accuracy and reproducibility of the test are ensured. The hollow transparent round tube has a diameter of 6-10cm and a length of 30-50cm.
In one embodiment of the application, the apparatus for evaluating endurance of fish body further comprises a flowmeter for detecting flow rate and flow velocity, wherein the flowmeter is arranged on a water pipe for communicating the water outlet of the tubular container with the water tank.
In an embodiment of the application, the device for evaluating endurance of the fish body further includes a nitrogen cylinder and an oxygen cylinder, wherein air outlets of air outlet pipes on the nitrogen cylinder and the oxygen cylinder are immersed in water in the water tank so as to supply oxygen and nitrogen to the water in the water tank; the nitrogen cylinder and the oxygen cylinder form dissolved oxygen amount regulating equipment, so that the influence of different dissolved oxygen environments on the endurance of the fish body can be simulated, and related data can be detected and collected.
In an embodiment of the application, the apparatus for evaluating endurance of a fish body further comprises a high-speed camera, wherein the high-speed camera is arranged above the tubular container to record the experimental phenomena in the tubular container.
In an embodiment of the application, the apparatus for evaluating endurance of a fish body further includes a refrigerator for cooling water in the water tank, wherein the refrigerator is used for cooling water in a fish pond or a fish tank;
the refrigerator is used as a water temperature adjusting device, can simulate the influence of different temperature environments on the endurance of the fish body, and can detect and collect related data;
The refrigerator is used for cooling water in a fish pond or a fish tank, is used for cooling water in the process of circulating water through the refrigerator in the prior art, has various types and structures, is commercially available, and can realize the control function without detailed description of the specific type and structure of the refrigerator;
As for the water heating in the water tank, the water heating function can be realized by referring to the water heating in the fish tank and placing a heating rod which is frequently used for the water heating of the fish tank in the prior art in the water tank.
In one embodiment of the application, the device for evaluating endurance of the fish body further comprises an interception net for soft interception of the fish, wherein the interception net is respectively arranged at the water inlet and the water outlet of the tubular container; the interception net is a porous net made of a material which does not damage the fish body (can not cause cutting or scratching), so that the fish can be prevented from being washed away, the fish body can not be cut or scratched, and the interception net is suitable for resting of the fish at the place. The mesh of the interception net is a honeycomb (regular hexagon) mesh.
In one embodiment of the application, the device for evaluating endurance of fish body further comprises a temperature sensor for detecting water temperature of water in the water tank, wherein a detection end of the temperature sensor is immersed in the water tank.
In one embodiment of the application, the device for evaluating endurance of fish body further comprises a sensor for detecting dissolved oxygen in water in the water tank, wherein a detection end of the dissolved oxygen sensor is immersed in the water tank.
Example 1: test of Effect of 0.1% ethanol solution on zebra fish endurance
1. Correcting the flow rate of the water body of the system: and starting the equipment, adjusting the water flow control valve to start the water pump to generate water flow, recording and testing a flow value corresponding to the frequency of the variable-frequency motor of the water pump, and observing, adjusting and correcting conditions through the flow control valve and the flowmeter.
2. Screening experimental zebra fish experimental fish groups: before the experiment, putting the zebra fish shoal into a hollow transparent circular tube filled with water, maintaining the anhydrous flow rate, enabling the zebra fish to adapt to the water for 3 minutes, gradually adjusting the water flow rate to 6.8BL/sec, adapting to 1 minute, gradually adjusting the water flow rate to 7.6BL/sec, and maintaining the water flow rate for 10 minutes, and screening the zebra fish experimental fish capable of completing the step.
3. Swimming endurance test: preparing 0.1% ethanol solution, designing a blank control group and a 0.1% ethanol solution experimental group, respectively changing the liquid in independent experimental cylinders into the two solutions, respectively placing two groups of zebra fish with equal quantity into the respective experimental cylinders for treating the to-be-detected object for 4 days, and feeding no fish during the treatment period of each group of fish for receiving the to-be-detected object. And (3) respectively placing each group of zebra fish after the treatment into a hollow transparent round tube filled with water for the adaptation training in the step (2). When the test of the continuous swimming time of the zebra fish is started, the motor is adjusted to the frequency corresponding to 7.6BL/sec in a variable frequency mode, and the swimming fatigue time of each fish is recorded.
4. And recording the swimming states of the two groups of zebra fishes in the test time by using a high-speed camera, and recording the continuous swimming time of the zebra fishes at the water flow rate of 7.6 BL/sec.
Analysis of the photographed video and specific experimental data can be seen as a result of the effect of 0.1% ethanol solution on zebra fish endurance (see fig. 3), as can be seen from fig. 3: the 0.1% ethanol solution has a great influence on the tolerance of zebra fish, and the swimming duration of the fish shoal can be reduced by 60%.
Example 2: effects of creatine and beta-alanine solutions on zebra fish endurance
1. Correcting the flow rate of the water body of the system: and starting the equipment, adjusting the water flow control valve to start the water pump to generate water flow, recording and testing a flow value corresponding to the frequency of the variable-frequency motor of the water pump, and observing, adjusting and correcting conditions through the flow control valve and the flowmeter.
2. Screening experimental zebra fish experimental fish groups: before the experiment, putting the zebra fish group into a hollow transparent circular tube filled with water, enabling the zebra fish to adapt for 3 minutes, gradually adjusting the water flow rate to 6.8BL/sec, adapting for 3 minutes, gradually adjusting the water flow rate to 7.6BL/sec, and maintaining for 10 minutes, and screening the zebra fish experimental fish capable of completing the step.
3. Swimming endurance test: the test liquids in independent test cylinders are respectively changed into three solutions, and zebra fish with equal quantity of the three groups are respectively put into the respective test cylinders for the treatment of the to-be-tested object for 4 days, and the fish of each group are not fed during the treatment period of the to-be-tested object. And (3) respectively placing each group of zebra fish after the treatment into a hollow transparent round tube filled with water for the adaptation training in the step (2). When the continuous swimming time test of the zebra fish shoal is started, the motor frequency conversion is adjusted to the frequency corresponding to 7.6BL/sec, and the swimming fatigue time of each fish is recorded.
4. Three groups of zebra fish swimming states in the test time are recorded by using a high-speed camera, and the continuous swimming time of the zebra fish at the water flow rate of 7.6BL/sec is recorded.
Analysis of the photographed video and specific experimental data can be seen as a result of the effect of 500. Mu.M creatine, 500. Mu.M beta. -alanine on zebra fish endurance (see FIG. 4), as can be seen from FIG. 4: the 500 mu M creatine and the 500 mu M beta-alanine can improve the endurance of the zebra fish, the continuous swimming time of the fish shoal is improved by 65% relative to the control group by using the 500 mu M creatine, the effect of the 500 mu M beta-alanine is better than that of the control group and the creatine group, and the continuous swimming time of the fish shoal is improved by more than 80% relative to the control group.
Example 3: comparison of maximum swimming endurance of zebra fish and catfish
1. Correcting the flow rate of the water body of the system: and starting the equipment, adjusting the water flow control valve to start the water pump to generate water flow, recording and testing a flow value corresponding to the frequency of the variable-frequency motor of the water pump, and observing, adjusting and correcting conditions through the flow control valve and the flowmeter.
2. Screening experimental zebra fish experimental fish groups:
Before the experiment, putting the zebra fish shoal into a hollow transparent circular tube filled with water, maintaining the anhydrous flow rate, enabling the zebra fish to adapt to 1 minute, gradually adjusting the water flow rate to 6.8BL/sec, adapting to 1 minute, gradually adjusting the water flow rate to 7.6BL/sec, and maintaining the water flow rate to 10 minutes, and screening the zebra fish experimental fish capable of completing the step.
Before the experiment, putting catfish shoal into a hollow transparent circular tube filled with water, maintaining the anhydrous flow rate, enabling catfish to adapt to 1 minute, gradually adjusting the water flow rate to 1.4BL/sec, adapting to 1 minute, gradually adjusting the water flow rate to 1.9BL/sec, and maintaining for 10 minutes, and screening catfish experimental fish capable of completing the step.
3. Swimming endurance test: the method comprises the steps of designing a zebra fish group and a catfish experimental group, respectively putting the two groups of zebra fish and catfish with equal quantity into respective experimental cylinders (all normal fish raising water) for 3 days, wherein the fishes of each group are not fed during the treatment period of receiving the to-be-detected object. And (3) respectively placing the zebra fish group and the catfish leases after the treatment into hollow transparent round tubes filled with water for the adaptation training in the step (2). When the continuous swimming time test of the experimental fish shoal is started, the zebra fish group adjusts the motor frequency conversion to the frequency corresponding to 7.6BL/sec, the catfish group adjusts the motor frequency conversion to the frequency corresponding to 1.9BL/sec, and the swimming fatigue time of each fish is recorded.
4. And recording the swimming states of the two groups of zebra fishes in the test time by using a high-speed camera, recording the continuous swimming time of the zebra fishes at the water flow rate of 7.6BL/sec, and recording the continuous swimming time of the catfishes at the water flow rate of 1.9 BL/sec.
The catfish was seen to have a 60% decrease in swimming duration relative to zebra fish (see figure 5) based on video recordings taken and specific experimental data analysis.
Example 4: effect of 1ppm ZnCl 2 solution on zebra fish endurance
1. Correcting the flow rate of the water body of the system: and starting the equipment, adjusting the water flow control valve to start the water pump to generate water flow, recording and testing a flow value corresponding to the frequency of the variable-frequency motor of the water pump, and observing, adjusting and correcting conditions through the flow control valve and the flowmeter.
2. Screening experimental zebra fish experimental fish groups: before the experiment, putting the zebra fish shoal into a hollow transparent circular tube filled with water, maintaining the anhydrous flow rate, enabling the zebra fish to adapt to the water for 3 minutes, gradually adjusting the water flow rate to 6.8BL/sec, adapting to 1 minute, gradually adjusting the water flow rate to 7.6BL/sec, and maintaining the water flow rate for 10 minutes, and screening the zebra fish experimental fish capable of completing the step.
3. Swimming endurance test: preparing 1ppm ZnCl 2 solution, designing a blank control group and a 1ppm ZnCl 2 solution experimental group, respectively changing the experimental liquid in independent experimental cylinders into the two solutions, respectively putting two groups of zebra fish with equal quantity into the respective experimental cylinders for 5 days, and not feeding the fish in each group during the treatment period of receiving the to-be-detected object. And (3) respectively placing each group of zebra fish after the treatment into a hollow transparent round tube filled with water for the adaptation training in the step (2). When the continuous swimming time test of the zebra fish shoal is started, the motor frequency conversion is adjusted to the frequency corresponding to 7.6BL/sec, and the swimming fatigue time of each fish is recorded.
4. And recording the swimming states of the two groups of zebra fishes in the test time by using a high-speed camera, and recording the continuous swimming time of the zebra fishes at the water flow rate of 7.6 BL/sec.
The effect of 1ppm ZnCl 2 solution on zebra fish endurance was analyzed according to the photographed video and specific experimental data (see FIG. 6), as can be seen from FIG. 6: the 1ppm ZnCl 2 solution has larger influence on the tolerance of zebra fish, and the swimming time of the fish shoal can be reduced by 50 percent.
Example 5: influence of 1ppm PbCl 2 on the endurance of zebra fish
1. Correcting the flow rate of the water body of the system: and starting the equipment, adjusting the water flow control valve to start the water pump to generate water flow, recording and testing a flow value corresponding to the frequency of the variable-frequency motor of the water pump, and observing, adjusting and correcting conditions through the flow control valve and the flowmeter.
2. Screening experimental zebra fish experimental fish groups: before the experiment, putting the zebra fish shoal into a hollow transparent circular tube filled with water, maintaining the anhydrous flow rate, enabling the zebra fish to adapt to the water for 3 minutes, gradually adjusting the water flow rate to 6.8BL/sec, adapting to 1 minute, gradually adjusting the water flow rate to 7.6BL/sec, and maintaining the water flow rate for 10 minutes, and screening the zebra fish experimental fish capable of completing the step.
3. Swimming endurance test: preparing 1ppm PbCl 2 solution, designing a blank control group and a 1ppm PbCl 2 solution experimental group, respectively changing the experimental liquid in independent experimental cylinders into the two solutions, respectively putting two groups of zebra fishes with equal quantity into the respective experimental cylinders for 4 days, and not feeding the fishes during the treatment period of the fishes to be tested. And (3) respectively placing each group of zebra fish after the treatment into a hollow transparent round tube filled with water for the adaptation training in the step (2). When the continuous swimming time test of the zebra fish shoal is started, the motor frequency conversion is adjusted to the frequency corresponding to 7.6BL/sec, and the swimming fatigue time of each fish is recorded.
4. And recording the swimming states of the two groups of zebra fishes in the test time by using a high-speed camera, and recording the continuous swimming time of the zebra fishes at the water flow rate of 7.6 BL/sec.
The effect of 1ppm PbCl 2 solution on zebra fish endurance was analyzed based on the recorded video and specific experimental data (see FIG. 7), as can be seen from FIG. 7: the solution 1ppmPbCl 2 has great influence on the endurance of zebra fish, and the swimming duration of the fish shoal can be reduced by 90 percent.
Example 6: influence of rhodiola rosea extract on zebra fish endurance
1. Correcting the flow rate of the water body of the system: and starting the equipment, adjusting the water flow control valve to start the water pump to generate water flow, recording and testing a flow value corresponding to the frequency of the variable-frequency motor of the water pump, and observing, adjusting and correcting conditions through the flow control valve and the flowmeter.
2. Screening experimental zebra fish experimental fish groups: before the experiment, putting the zebra fish shoal into a hollow transparent circular tube filled with water, maintaining the anhydrous flow rate, enabling the zebra fish to adapt to the water for 3 minutes, gradually adjusting the water flow rate to 6.8BL/sec, adapting to the water for 3 minutes, gradually adjusting the water flow rate to 7.6BL/sec, and maintaining the water for 10 minutes, and screening the zebra fish experimental fish capable of completing the step.
3. Swimming endurance test: the test liquid in the endurance testing equipment is respectively changed into the three solutions, and the three groups of zebra fishes with equal quantity are respectively put into the respective test cylinders for 4 days, and are not fed during the treatment period of the fish to be tested. And (3) respectively placing each group of zebra fish after the treatment into a hollow transparent round tube filled with water for the adaptation training in the step (2). When the continuous swimming time test of the zebra fish shoal is started, the motor frequency conversion is adjusted to the frequency corresponding to 7.6BL/sec, and the swimming fatigue time of each fish is recorded.
4. Three groups of zebra fish swimming states in the test time are recorded by using a high-speed camera, and the continuous swimming time of the zebra fish at the water flow rate of 7.6BL/sec is recorded.
The effect of rhodiola rosea on zebra fish endurance is analyzed according to the photographed video and specific experimental data (see fig. 8), and it can be seen from fig. 8: the rhodiola rosea can improve the endurance of zebra fish, the experimental group using 10ppm rhodiola rosea extract is improved by 70% relative to the continuous swimming time of the fish shoal of the control group, the effect of using 10ppm rhodiola rosea extract is better than that of the control group and the 10ppm rhodiola rosea extract, and the continuous swimming time of the fish shoal of the control group is improved by 85%.
Example 7: effect of plasticizer di (2-ethyl) hexyl phthalate on zebra fish endurance
1. Correcting the flow rate of the water body of the system: and starting the equipment, adjusting the water flow control valve to start the water pump to generate water flow, recording and testing a flow value corresponding to the frequency of the variable-frequency motor of the water pump, and observing, adjusting and correcting conditions through the flow control valve and the flowmeter.
2. Screening experimental zebra fish experimental fish groups: before the experiment, putting the zebra fish shoal into a hollow transparent circular tube filled with water, maintaining the anhydrous flow rate, enabling the zebra fish to adapt to the water for 3 minutes, gradually adjusting the water flow rate to 6.8BL/sec, adapting to the water for 3 minutes, gradually adjusting the water flow rate to 7.6BL/sec, and maintaining the water for 10 minutes, and screening the zebra fish experimental fish capable of completing the step.
3. Swimming endurance test: a blank, a test group of 1ppm di (2-ethyl) hexyl phthalate and a test group of 10ppm di (2-ethyl) hexyl phthalate were designed, the test liquids in the independent test cylinders were respectively changed to these three solutions, and three groups of zebra fish of equal number were respectively placed in the respective test cylinders for 4 days, and each group of fish was not fed during the treatment period in which the test object was received. And (3) respectively placing each group of zebra fish after the treatment into a hollow transparent round tube filled with water for the adaptation training in the step (2). When the continuous swimming time test of the zebra fish shoal is started, the motor frequency conversion is adjusted to the frequency corresponding to 7.6BL/sec, and the swimming fatigue time of each fish is recorded.
4. Three groups of zebra fish swimming states in the test time are recorded by using a high-speed camera, and the continuous swimming time of the zebra fish at the water flow rate of 7.6BL/sec is recorded.
The effect of the plasticizer di (2-ethyl) hexyl phthalate on zebra fish endurance was analyzed based on the recorded video and specific experimental data (see fig. 9), as can be seen from fig. 9: di (2-ethyl) hexyl phthalate reduced zebra fish endurance, 45% of the continuous swimming time for the control group fish population was reduced by the experimental group using 1ppm of di (2-ethyl) hexyl phthalate, and 70% of the continuous swimming time for the control group fish population was reduced by the experimental group using 10ppm of di (2-ethyl) hexyl phthalate. Zebra fish can have reduced endurance in the contaminant water.
Example 8: influence of nano fullerene C60 on zebra fish endurance
1. Correcting the flow rate of the water body of the system: and starting the equipment, adjusting the water flow control valve to start the water pump to generate water flow, recording and testing a flow value corresponding to the frequency of the variable-frequency motor of the water pump, and observing, adjusting and correcting conditions through the flow control valve and the flowmeter.
2. Screening experimental zebra fish experimental fish groups: before the experiment, putting the zebra fish shoal into a hollow transparent circular tube filled with water, maintaining the anhydrous flow rate, enabling the zebra fish to adapt to the water for 3 minutes, gradually adjusting the water flow rate to 6.8BL/sec, adapting to the water for 3 minutes, gradually adjusting the water flow rate to 7.6BL/sec, and maintaining the water for 10 minutes, and screening the zebra fish experimental fish capable of completing the step.
3. Swimming endurance test: a blank control group, an experimental group of 0.5ppm nano fullerene C60 and an experimental group of 2ppm nano fullerene C60 were designed, the test liquid in the endurance testing equipment was changed to the three solutions, and three groups of zebra fish with equal quantity were placed in the respective experimental cylinders for 4 days, and the fish of each group was not fed during the treatment of the test object. And (3) respectively placing each group of zebra fish after the treatment into a hollow transparent round tube filled with water for the adaptation training in the step (2). When the continuous swimming time test of the zebra fish shoal is started, the motor frequency conversion is adjusted to the frequency corresponding to 7.6BL/sec, and the swimming fatigue time of each fish is recorded.
4. Three groups of zebra fish swimming states in the test time are recorded by using a high-speed camera, and the continuous swimming time of the zebra fish at the water flow rate of 7.6BL/sec is recorded.
The effect of nano fullerene C60 on zebra fish endurance was analyzed according to the photographed videos and specific experimental data (see fig. 10), and it can be seen from fig. 10: nano-fullerene C60 reduced zebra fish endurance, 18% of the continuous swimming time of the fish population with the control group, and 70% of the continuous swimming time of the fish population with the control group, with the experimental group using 0.5ppm nano-fullerene C60. The endurance of the zebra fish in the pollutant water body is reduced, and the continuous swimming time is reduced along with the increase of the pollutant content.
Claims (5)
1.A method for evaluating the influence of a substance to be tested on the endurance of body movement is characterized in that,
An apparatus for evaluating endurance of a fish body is used, which comprises a water tank, a water pump, a tubular container for fish to swim in, a high-speed camera and a regulating valve for switching a waterway and regulating the flow;
The water inlet of the water pump is communicated with the water tank for pumping water from the water tank, the water outlet of the water pump is communicated with the water inlet of the tubular container through a water pipe, the water outlet of the tubular container is communicated with the water tank through a water pipe for returning water to the water tank, and an interception net for soft interception of fish is respectively arranged at the water inlet and the water outlet of the tubular container;
the regulating valve is arranged on a water pipe which is communicated with the water outlet of the water pump and the water inlet of the tubular container, and the flowmeter is arranged on the water pipe which is communicated with the water outlet of the tubular container and the water tank;
the high-speed camera is arranged above or/and at the side of the tubular container so as to carry out shooting record on experimental phenomena in the tubular container;
The tubular container is a hollow transparent round tube;
The method for evaluating the influence of the substance to be tested on the exercise endurance of the organism specifically comprises the following steps:
step 1: correcting the flow rate of the water body of the system;
Step 2: experimental fish swarm screening: before the experiment, putting the fish school into water in a tubular container to maintain the anhydrous flow rate to adapt the fish to 3-5 minutes, slowly increasing the water flow rate to 1.4-6.8 BL/sec for 1-3 minutes, slowly increasing the water flow rate to 1.9-7.6 BL/sec for 10-15 minutes, and selecting experimental fish capable of completing the training;
step 3: swimming endurance test: preparing or/and collecting water body containing the object to be detected as an experimental group, and setting normal water without the object to be detected as a control group; the number of the fishes in each group is 7-10, the experimental fishes are respectively put into independent experimental cylinders to be treated by the object to be tested for 3-5 days, and the fishes in each group are not fed during the treatment period of the object to be tested; placing each group of experimental fish shoals after treatment into water in a tubular container for adaptive training in the step 2; when starting to detect the continuous swimming time test of the experimental fish shoal, adjusting the motor frequency conversion to the frequency corresponding to the required flow, and recording the swimming fatigue time;
The swimming fatigue is that the swimming is kept in a countercurrent manner in a tubular container until the swimming is pushed by water flow to stop for more than 3s by an interception net;
Step 4: experimental data collection and result analysis: in the experimental process, the water flow rate and the swimming fatigue time of the experimental fish school are recorded, and a statistical tool is used for carrying out statistical analysis on an experimental group and a control group of the object to be detected so as to judge the influence of the object to be detected on the swimming movement capability of the fish body.
2. The method of evaluating the effect of a test substance on physical exercise tolerance of claim 1, wherein the test substance is an anti-fatigue drug, an anti-fatigue nutrient, or a contaminant.
3. The method for evaluating the effect of a substance to be tested on the endurance of a body of a person to exercise according to claim 1, wherein the mesh of the interception net is a honeycomb mesh, and the interception net is made of a plastic material of soft interception fish.
4. The method for evaluating the influence of a substance to be tested on the exercise endurance of a body according to claim 1, wherein the device for evaluating the endurance of a fish body further comprises a nitrogen cylinder and an oxygen cylinder, and air outlets of air outlet pipes on the nitrogen cylinder and the oxygen cylinder are immersed in water in the water tank so as to supply oxygen and nitrogen to the water in the water tank.
5. The method according to claim 4, wherein the apparatus for evaluating the endurance of the fish body further comprises a refrigerator for cooling water in the water tank, the refrigerator is a circulating water type refrigerator for cooling water in the fish pond or the fish tank, and a circulating water inlet and a circulating water outlet of the refrigerator are both communicated with the water tank to form a circulating water loop.
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| CN111802307B (en) * | 2020-07-29 | 2022-03-29 | 湖南工商大学 | Zebra fish training device |
| CN112129901B (en) * | 2020-08-31 | 2023-01-17 | 南京新环检测科技有限公司 | Method for evaluating sports nutritive value of food-derived anti-fatigue active ingredients |
| CN113080137A (en) * | 2021-04-02 | 2021-07-09 | 扬州大学 | Experimental method for researching nutrition factors for improving exercise endurance of training rat or mouse |
| CN113648298A (en) * | 2021-08-18 | 2021-11-16 | 上海市同仁医院 | Application of creatine in preparing composition for inhibiting white fat cell differentiation |
| CN114680069B (en) * | 2021-11-19 | 2022-12-06 | 杭州环特生物科技股份有限公司 | Zebra fish behavior and movement experimental device |
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