CN108782227A - A kind of breeding method of high grain weight blast resisting two-line hybrid rice restorer - Google Patents
A kind of breeding method of high grain weight blast resisting two-line hybrid rice restorer Download PDFInfo
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- CN108782227A CN108782227A CN201810719228.4A CN201810719228A CN108782227A CN 108782227 A CN108782227 A CN 108782227A CN 201810719228 A CN201810719228 A CN 201810719228A CN 108782227 A CN108782227 A CN 108782227A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The present invention discloses a kind of breeding method of high grain weight blast resisting two-line hybrid rice restorer, including with Yang No.6 rice for parent 1, with paddy plum, No. 4 hybridize to obtain F for parent 21For seed；F1It is returned with parent 1 for after shading treatment, obtains BC1F1For seed；Plant BC1F1For seed, selection can normally ear, is returned containing rice blast resistance gene and with 1 character similar individuals of parent and parent 1, obtain BC2F1For seed；Plant BC2F1Generation, selection obtain BC containing rice blast resistance gene and individual similar with 1 character of parent and the backcrossing of parent 13F1For seed；Plant BC3F1In generation, selection is containing rice blast resistance gene and individual selfing acquisition BC similar with 1 character of parent3F2For seed；Plant BC3F2In generation, selects the individual of mass of 1000 kernel 30g or more, plants BC3F3Homozygous, other economical characters and yield performance and the almost the same strain of parent 1 for selection target rice blast resistance gene.
Description
Technical field
The present invention relates to rice breeding technology field more particularly to a kind of high grain weight blast resisting two-line hybrid rice restorers
Breeding method.
Background technology
Rice is one of most important cereal crops in China, to ensureing that China's grain security plays an important roll.China's water
The improvement of rice varieties yield potentiality, after two important stages of breeding wheat for semidwarfness and heterosis utilization.Wherein, with hybrid rice
Breed of variety is that the heterosis utilization of core is still so far the important technical of rice varieties improvement, and occupies in the whole world leading
Status.Breeding of hybridized rice is divided into three line method, two line method, and two systems by Rescued virus due to not restricted, having combo free
Feature and receive numerous breeders and praised highly.Currently, the popularization and application of double-linear hybrid rice have accounted for China hybrid rice
The half of the country of kind cultivated area, and still in ascent stage.Therefore, reinforce improvement and the germplasm wound of two-line hybrid rice parent
It makes significant.
The rice blast caused by Pyricularia oryzae (Magnaporthe oryzae) is one of Main Rice Diseases, is seriously threatened
The safety in production of world food.Since the 1990s, Chinese rice blast year occurring area is in 3,800,000 hm2More than, directly
It connects and the loss of paddy year is caused to reach several hundred million kilograms.It was verified that being to control this disease most to pass through using resistant gene, cultivation disease-resistant variety
It helps effective method.But the number gene that rice seed is related to rice blast resistance is numerous, so far, at least 100 or so rice blasts
Sick resistant gene/site is positioned, and has 27 rice blast resistance genes to be cloned (national rice data center, http://
www.ricedata.com).Moreover, because the genetic complexity and mutability of rice blast biological strain, contain single resistance base
Will lose resistance after the kind plantation 3-5 of cause leads to production loss.Therefore, broad spectrum durable resistant gene is excavated and utilizes,
It is the key technology for cultivating durable resistance kind.
Pigm is the wide spectrum rice blast resistance gene that China scientist excavates from local varieties paddy No. 4 kinds of plum, application
There is good application effect in rice blast resistance improvement, but the gene often brings granule effect (Deng Y, Zhai simultaneously
K,Xie Z,Yang D,Zhu X,Liu J,Wang X,Qin P,Yang Y,Zhang G,Li Q,Zhang J,Wu S,
Milazzo J,Mao B,Wang E,Xie H,Tharreau D,He Z.Epigenetic regulation of
antagonistic receptors confers rice blast resistance with yield
balance.Science,2017,355:1-4), the promotion of variety yield potentiality is limited, while reducing the commodity of rice
Property.Using the target gene, the rice breed of high grain weight, blast resisting is formulated, there is significant application value.
Invention content
The purpose of the present invention is to provide a kind of breeding methods of high grain weight blast resisting two-line hybrid rice restorer, are bred as
Sizing strain polymerize 2 kinds of high grain weight, blast resisting characters, can be effectively used for strong advantage, the combination of blast resisting two-line hybrid rice
Cultivation.
A kind of breeding method of high grain weight blast resisting two-line hybrid rice restorer, including：
(1) it is parent 1 to select high grain weight, strong coordinate force two-line hybrid rice restorer, selects blast resisting place long-grained nonglutinous rice product
Kind is parent 2, is hybridized, and F is obtained1For seed.
(2) F is planted1Generation, and shading treatment is carried out, it is returned with parent 1 after heading, obtains BC1F1For seed.
(3) BC plants in big group1F1Generation, marker assisted selection carry target rice blast resistance gene and show heading it is normal,
It is returned with parent 1 with the most similar individual of recurrent parent, obtains BC2F1For seed.
(4) BC is planted2F1Generation, label selection carry target rice blast resistance gene and with recurrent parent economical character the most
Similar individual is returned with parent 1, obtains BC3F1For seed.
(5) BC is planted3F1Generation, label selection carry target rice blast resistance gene and with recurrent parent economical character the most
Similar individual selfing obtains BC3F2For seed.
(6) big group mixed planting BC3F2In generation, selects the individual of mass of 1000 kernel 30g or more, obtains the BC of different single plants3F3Generation
Seed.
(7) BC is planted3F3In generation, label selection target rice blast resistance gene is homozygous and other economical characters and yield show
With the almost the same strain of parent 1 to get high grain weight blast resisting two-line hybrid rice restorer.
In step (1), with parent 1 it is maternal, is that male parent hybridizes with parent 2；In step (2), with F1On behalf of it is maternal,
It is that male parent is returned with parent 1；In step (3), with BC1F1It is that male parent is returned on behalf of female parent, with parent 1；Step (4)
In, with BC2F1It is that male parent is returned on behalf of female parent, with parent 1.
Preferably, the parent 1 is Yang No.6 rice (code name of participating in the experiment, 9311).
Yang No.6 rice is that Inst. of Agricultural Science, Lixiahe Prefecture, Jiangsu Prov. cultivates.The kind is to Ministry of Agriculture's plant new product
Kind protects office to apply and authorize, kind power number：CAN20000091.8 can be purchased from Jiangsu gold soil Zhong Ye Co., Ltds.It should
Kind is the elite restorer lines of China's two-line hybrid rice, and prepared hybrid paddy rice has yield height, the excellent, wide adaptability of quality etc. excellent
Point.
Preferably, the parent 2 is paddy plum No. 4.
Paddy plum No. 4 is China place rice variety, carries rice blast resistance gene Pigm, is China's performance wide spectrum, lasting
The elite germplasm of rice blast resistance.It refers to：Deng Y,Zhai K,Xie Z,Yang D,Zhu X,Liu J,Wang X,Qin
P,Yang Y,Zhang G,Li Q,Zhang J,Wu S,Milazzo J,Mao B,Wang E,Xie H,Tharreau D,He
Z.Epigenetic regulation of antagonistic receptors confers rice blast
resistance with yield balance.Science,2017,355:1-4。
Yang No.6 rice and paddy plum No. 4 is selected to hybridize, be returned, binding molecule marker assisted selection and big mass screening are cultivated
High grain weight blast resisting two-line hybrid rice restorer.The strain induces identification through artificial infection and sick nursery, to tables such as seedling pest, fringe pests
Existing good resistance；Its mass of 1000 kernel is up to 30.5g, suitable with the Yang No.6 rice of mass of 1000 kernel 30.1g, overcomes and carries Pigm genes
The usually relatively low defect of rice material grain weight.
Preferably, in step (3), (4), (5), (7), the molecular labeling of assisted Selection is InDel 587.
587 primer sequences of InDel and PCR reaction operating method referring to：Pan Cunhong etc., one kind is for detecting paddy plum No. 4
The molecular labeling InDel 587 of blast resistant gene Pigm (t), the patent No.：ZL 201310428162.0
Preferably, in step (2), shading treatment refers to after transplanting one month, is surrounded in morning and evening black cloth and iron wire
Cover is by F1It is covered for plant, shut out the light irradiation, under summer high temperature, long-day conditions, keeps F1Receive light daily for plant
According to time 7-8 hour, 20 days shading treatment time.
Preferably, in step (3), BC plants in big group1F1In generation, refers to population size and is not less than 100 plants.
Preferably, in step (6), big group's mixed planting BC3F2In generation, refers to population size and is not less than 1000 plants.
Preferably, in step (6), big group's mixed planting BC3F2In generation, selects the individual of mass of 1000 kernel 30g or more, refers to a
Body list seedling is planted, and single plant is divided to examine or check mass of 1000 kernel.
Compared with prior art, beneficial effects of the present invention are：
(1) rice restorer that the present invention is bred as, the good characteristic with high grain weight, blast resisting, effective for strong excellent
The cultivation that gesture, blast resisting two-line hybrid rice combine.
(2) present invention is in F1In generation, carries out shading treatment, solves Yang No.6 rice and hybridizes for No. 4 with paddy plum, hybrid F1In high temperature, length
The problem do not eared under sunshine condition and cannot be returned.
(3) BC plants in the big group of the present invention1F1In generation, selection heading is normal and carries the individual of target rice blast resistance gene
It is returned, can break chain between slow breeding time gene and rice blast resistance gene, overcome burdensome chain effect.
(4) the big group mixed planting BC of the present invention3F2In generation, selects the individual selfing of mass of 1000 kernel 30g or more to form offspring's strain
System overcomes and carries the progeny material of wide spectrum rice blast ospc gene Pigm and usually show the defect of granule, improve yield potentiality with
The commodity performance of rice.
Description of the drawings
The breeding method schematic diagram of Fig. 1 high grains weight blast resisting two-line hybrid rice restorer
Specific implementation mode
With reference to embodiment, present invention is further described in detail.
1, breeding material：
(1) it is female parent with Yang No.6 rice.Yang No.6 rice is that Inst. of Agricultural Science, Lixiahe Prefecture, Jiangsu Prov. cultivates.The kind
Apply and authorize to Plant new variety protection office of the Ministry of Agriculture, kind power number：CAN20000091.8 can be purchased from Jiangsu gold
Soil Zhong Ye Co., Ltds.The kind is the elite restorer lines of China's two-line hybrid rice, prepared hybrid paddy rice have yield it is high,
The advantages that quality is excellent, wide adaptability.
(2) with paddy plum No. 4 for male parent.Paddy plum No. 4 is China place rice variety, carries rice blast resistance gene Pigm,
It is the elite germplasm of China's performance wide spectrum, lasting rice blast resistance.It refers to：Deng Y,Zhai K,Xie Z,Yang D,Zhu
X,Liu J,Wang X,Qin P,Yang Y,Zhang G,Li Q,Zhang J,Wu S,Milazzo J,Mao B,Wang E,
Xie H,Tharreau D,He Z.Epigenetic regulation of antagonistic receptors confers
rice blast resistance with yield balance.Science,2017,355:1-4。
2, the Breeding Process of high grain weight blast resisting two-line hybrid rice restorer is：
(1) 2008-2009 winter-spring seasons, Hainan Teng Qiao, with high grain weight two-line hybrid rice restorer Yang No.6 rice (parent 1)
For female parent, hybridize with blast resisting place rice variety paddy plum No. 4 (parents 2), obtains F1For 27, seed.
F is planted in (2) 2009 year summer, Jiangsu Yangzhou1Plant, with black cloth to F after transplanting one month1When plant shading treatment
Between 20 days (keep daily illumination 7-8 hour), with Yang No.6 rice (parent 1) be that male parent is returned 1 time after heading, acquisition is as more as possible
Obtain BC1F1135, seed.
BC is planted in (3) 2010 year summer, Jiangsu Yangzhou1F1For 123 plants of plant, the individual that can normally ear is selected, is utilized
Molecular labeling InDel 587 detects whether carrying Pigm genes, and final choice heading is normal, carries target rice blast resistance gene
And show and be returned with Yang No.6 rice (parent 1) for the most similar individual 3 plants with recurrent parent, obtain BC2F1For 56, seed.
(4) 2010-2011 winter-spring seasons, Hainan Teng Qiao plant BC2F1In 48 plants of generation, is examined using molecular labeling InDel 587
Whether survey carries Pigm genes, and selection carries target rice blast resistance gene and shows and recurrent parent the most similar individual 8
Strain is returned with Yang No.6 rice (parent 1), obtains BC3F1For 122, seed.
(5) 2011 year summer, Jiangsu Yangzhou detect whether to carry Pigm genes, selection using molecular labeling InDel 587
It carries target rice blast resistance gene and shows and obtain BC with the most similar individual 6 plants of recurrent parent, selfing3F2For seed.
(6) 2011-2012 winter-spring seasons, Hainan Teng Qiao, mixed planting BC3F2For 1500 plants of group, seed is harvested by single plant,
Mass of 1000 kernel is examined or check, 17 plants of the individual of mass of 1000 kernel 30g or more is selected, obtains BC3F3For seed.
BC is planted in (7) 2012 years Summer and winters, Jiangsu Yangzhou by strain3F3In generation, being detected using molecular labeling InDel 587 is
No carrying Pigm genes, selection target rice blast resistance gene is homozygous and other economical characters and yield show and Yang No.6 rice base
This consistent strain 2.Obtain the two-line hybrid rice restorer of high grain weight blast resisting.
Summer Jiangsu Yangzhou in 2013 and 2013-2014 winter-spring seasons Hainan Teng Qiao, two strains that this implementation is obtained
Carry out yield product than identification by strain, as a result the plantation of three replicated plots shows its yield level and production per 0.02 mu of plot area
It is suitable with control Yang No.6 rice to measure Components, mass of 1000 kernel is stablized at 30.5 grams or more (tables 1).
2013 and summer in 2014, Jiangsu Yangzhou, using the representative bacterial strain of separate sources, two that this implementation is obtained
A strain carries out the artificial infection idenfication of seedling pest and fringe pest, as a result shows and shows disease-resistant, is significantly better than control Yang No.6 rice (table
2)。
2015-2017 retransmits area in continuous 3 years throughout the year in rice blast such as Fujian Jianyang, Jinggangshan County, Jiangxi Province, Mt. Huang in Anhui,
As a result the natural inoculated identification that two strains that this implementation obtains are carried out with rice blast shows and shows disease-resistant, is significantly better than pair
According to Yang No.6 rice (table 3).
Technical scheme of the present invention and advantageous effect is described in detail in embodiment described above, it should be understood that
Above is only a specific embodiment of the present invention, it is not intended to restrict the invention, it is all to be done in the spirit of the present invention
Any modification, supplementary, and equivalent replacement etc., should all be included in the protection scope of the present invention.
1, table implements the yield and its components of the 2 high grain obtained weight blast resisting two-line hybrid rice restorer
2, table implements the 2 high grain obtained weight blast resisting two-line hybrid rice restorer rice blast artificial infection Resistant expression
3, table implements the 2 high grain obtained weight blast resisting two-line hybrid rice restorer natural rice blast and induces identification resistance table
It is existing
Claims (7)
1. a kind of breeding method of high grain weight blast resisting two-line hybrid rice restorer, which is characterized in that including：
(1) it is parent 1 to select high grain weight, strong coordinate force two-line hybrid rice restorer, select blast resisting place rice variety for
Parent 2, hybridized, and F is obtained1For seed；
(2) F1 generation is planted, and carries out shading treatment, is returned with parent 1 after heading, BC is obtained1F1For seed；
(3) BC plants in big group1F1In generation, selection heading is normal, rice blast resistance gene containing target, the most similar to recurrent parent
Individual with parent 1 be returned, obtain BC2F1For seed；
(4) BC is planted2F1Generation, select rice blast resistance gene containing target and with the most similar individual of recurrent parent economical character
It is returned with parent 1, obtains BC3F1For seed；
(5) BC is planted3F1Generation, select rice blast resistance gene containing target and with the most similar individual of recurrent parent economical character
Selfing obtains BC3F2For seed；
(6) big group mixed planting BC3F2In generation, selects the individual of mass of 1000 kernel 30g or more, obtains the BC of different single plants3F3For seed；
(7) BC is planted3F3Generation, selection target rice blast resistance gene homozygosis, other economical characters and yield performance and 1 base of parent
This consistent strain is to get high grain weight blast resisting two-line hybrid rice restorer.
2. the breeding method of high grain weight blast resisting two-line hybrid rice restorer according to claim 1, which is characterized in that
The parent 1 is Yang No.6 rice；Parent 2 is paddy plum No. 4.
3. the breeding method of high grain weight blast resisting two-line hybrid rice restorer according to claim 1 or 2, feature exist
In in step (1), with parent 1 being maternal, be that male parent hybridizes with parent 2；It is maternal, with parent with F1 generation in step (2)
Originally it 1 is returned for male parent；In step (3), with BC1F1It is that male parent is returned on behalf of female parent, with parent 1；In step (4), with
BC2F1It is that male parent is returned on behalf of female parent, with parent 1.
4. the breeding method of high grain weight blast resisting two-line hybrid rice restorer according to claim 2, which is characterized in that
In step (3), (4), (5), (7), target rice blast resistance gene is Pigm, and detection is labeled as InDel 587.
5. the breeding method of high grain weight blast resisting two-line hybrid rice restorer according to claim 1 or 2, feature exist
In in step (2), shading treatment refers to after transplanting one month, in the cover that morning and evening black cloth and iron wire surround by F1For plant
It covers, shut out the light irradiation, under summer high temperature, long-day conditions, keeps F1It is small to receive light application time 7-8 daily for plant
When, 20 days shading treatment time.
6. the breeding method of high grain weight blast resisting two-line hybrid rice restorer according to claim 1 or 2, feature exist
In in step (3), BC plants in big group1F1The population size in generation is not less than 100 plants.
7. the breeding method of high grain weight blast resisting two-line hybrid rice restorer according to claim 1 or 2, feature exist
In, in step (6), big group's mixed planting BC3F2The population size in generation is not less than 1000 plants.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103497996A (en) * | 2013-09-22 | 2014-01-08 | 江苏里下河地区农业科学研究所 | Molecular marker InDe1587 for detecting rice blast resistant gene Pigm(t) of rice #4 |
| CN104969855A (en) * | 2015-07-30 | 2015-10-14 | 江苏里下河地区农业科学研究所 | Method for cultivating rice breeding material with broad spectrum and lasting spike blast resistance |
-
2018
- 2018-07-03 CN CN201810719228.4A patent/CN108782227A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103497996A (en) * | 2013-09-22 | 2014-01-08 | 江苏里下河地区农业科学研究所 | Molecular marker InDe1587 for detecting rice blast resistant gene Pigm(t) of rice #4 |
| CN104969855A (en) * | 2015-07-30 | 2015-10-14 | 江苏里下河地区农业科学研究所 | Method for cultivating rice breeding material with broad spectrum and lasting spike blast resistance |
Non-Patent Citations (2)
| Title |
|---|
| 李进波等: "水稻抗稻瘟病基因Pi1 和Pi2 聚合系的获得及其抗性评价", 《安徽农业科学》 * |
| 殷得所等: "抗稻瘟病基因Pi9的STS连锁标记开发及在分子标记辅助育种中的应用", 《中国水稻科学》 * |
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Application publication date: 20181113 |