CN108760674A - A kind of terahertz time-domain spectroscopy detection device for detecting biological neural sample - Google Patents
A kind of terahertz time-domain spectroscopy detection device for detecting biological neural sample Download PDFInfo
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- CN108760674A CN108760674A CN201810561830.XA CN201810561830A CN108760674A CN 108760674 A CN108760674 A CN 108760674A CN 201810561830 A CN201810561830 A CN 201810561830A CN 108760674 A CN108760674 A CN 108760674A
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- 238000001514 detection method Methods 0.000 title claims abstract description 80
- 230000001537 neural effect Effects 0.000 title claims abstract description 49
- 238000001328 terahertz time-domain spectroscopy Methods 0.000 title claims abstract description 25
- 210000005036 nerve Anatomy 0.000 claims abstract description 142
- 238000001228 spectrum Methods 0.000 claims abstract description 19
- 239000001257 hydrogen Substances 0.000 claims abstract description 18
- 229910052739 hydrogen Inorganic materials 0.000 claims abstract description 18
- 238000007405 data analysis Methods 0.000 claims abstract description 13
- 238000004519 manufacturing process Methods 0.000 claims abstract description 11
- 238000004611 spectroscopical analysis Methods 0.000 claims abstract description 9
- 230000000694 effects Effects 0.000 claims abstract description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 42
- 229910052757 nitrogen Inorganic materials 0.000 claims description 21
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 13
- 229910052710 silicon Inorganic materials 0.000 claims description 13
- 239000010703 silicon Substances 0.000 claims description 13
- 230000006835 compression Effects 0.000 claims description 12
- 238000007906 compression Methods 0.000 claims description 12
- 229910001220 stainless steel Inorganic materials 0.000 claims description 11
- 239000010935 stainless steel Substances 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 8
- 238000005192 partition Methods 0.000 claims description 8
- 239000004698 Polyethylene Substances 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- -1 polyethylene Polymers 0.000 claims description 6
- 229920000573 polyethylene Polymers 0.000 claims description 6
- 208000002925 dental caries Diseases 0.000 claims description 5
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 4
- 229910052802 copper Inorganic materials 0.000 claims description 4
- 239000010949 copper Substances 0.000 claims description 4
- 230000005611 electricity Effects 0.000 claims description 4
- 230000000644 propagated effect Effects 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 abstract description 3
- 230000009931 harmful effect Effects 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 44
- 238000005516 engineering process Methods 0.000 description 20
- 239000003570 air Substances 0.000 description 12
- 238000010586 diagram Methods 0.000 description 8
- 230000008014 freezing Effects 0.000 description 4
- 238000007710 freezing Methods 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 239000012472 biological sample Substances 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000012080 ambient air Substances 0.000 description 2
- 230000001427 coherent effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000000149 penetrating effect Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000006303 photolysis reaction Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000002310 reflectometry Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3581—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using far infrared light; using Terahertz radiation
- G01N21/3586—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using far infrared light; using Terahertz radiation by Terahertz time domain spectroscopy [THz-TDS]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
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- Spectroscopy & Molecular Physics (AREA)
- Toxicology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The present invention relates to a kind of terahertz time-domain spectroscopy detection device for detecting biological neural sample, described device includes：Nerve samples low temperature environment manufacturing cell, for providing the low temperature environment needed for detection nerve samples terahertz light time spectrum, by the way that nerve samples are cooled to 77K, the freedom of motion of hydrogen bond is set to freeze, to evade harmful effect of the hydrogen bond of hydrone formation in sample to spectrum；THz wave generates unit, the broadband THz wave for generating detection nerve samples information；THz wave for detecting the broadband THz wave for carrying relevant information after nerve samples, and is converted to electric signal and obtains time-domain spectroscopy by THz wave detection unit；Data analysis unit, the time-domain spectroscopy data for being obtained to the THz wave detection unit carry out Fourier transform and obtain frequency domain spectra.The present invention can fundamentally evade the interference effect that contained humidity in biological neural sample forms hydrogen bond to terahertz signal, have highly sensitive, signal-to-noise ratio and the characteristics such as stability is good.
Description
Technical field
The present invention relates to a kind of detection devices detecting biological neural sample using terahertz time-domain spectroscopic technology, especially
It is that the detection device is designed according to basic physical principle, can fundamentally evades the hydrogen that hydrone is formed in biological neural sample
Key has many advantages, such as that high sensitivity, signal-to-noise ratio and stability are good to the interference effect of spectrum.
Background technology
Terahertz (Terahertz or THz) wave is commonly referred to as the electromagnetic wave that frequency is between 0.1THz-10THz,
Its photon energy is very low, will not cause biomolecule photodissociation, has extraordinary safety.Terahertz time-domain spectroscopy
(terahertz time domain spectroscopy, THz-TDS) technology is a kind of coherent detection technology, can be obtained simultaneously
The amplitude information and phase information for obtaining terahertz signal can directly obtain sample by carrying out Fourier transform to time waveform
The optical parameters such as absorption coefficient and refractive index.Terahertz time-domain spectroscopic technology is due to its highly sensitive, high s/n ratio and high score
The excellent specific properties such as resolution, have been widely used in the research of life science.Correlation theory and experimental study show biomolecule
Rotational energy level be located at 0.001-0.3cm-1, interior rotational level is located at 0.3-11cm-1, low-frequency vibration energy grade is located at 11-250cm-1, it is in terahertz wave band.Therefore, THz wave becomes the ideal tools for probing into biomolecule and life science, this is just profit
Possibility is provided with terahertz time-domain spectroscopic technology detection biological neural sample.
Terahertz time-domain spectroscopic technology the biomolecule detections such as protein, amino acid field obtained it is good development and
Using, and have been developed that and its Nondestructive Identification technology is carried out based on terahertz time-domain spectroscopic technology.But it is direct using the technology
When carrying out biological neural sample analysis, great difficulty is encountered.Due to containing a certain amount of hydrone in biological neural sample,
Hydrogen bond can be formed between hydrone, the rotation and vibration of hydrogen bond can generate very strong absorption to THz wave, lead to biological neural sample
The Terahertz characteristic absorption spectrum of product is interfered by hydrone, it is difficult to be identified.Meanwhile utilizing the Terahertz of femtosecond laser pump excitation
The case where intensity of wave is limited, and the interference of hydrone can even cause signal to be buried completely by background signal occurs.These factors make
It obtains and receives prodigious restriction using terahertz time-domain spectroscopic technology detection biological neural sample, limit the further of the technology
Development and widely application.Currently, this problem is directed to, both at home and abroad still without feasible solution.
Invention content
Present invention mainly solves existing technologies when existing terahertz time-domain spectroscopic technology detects biological neural sample to ask
Cryogenic freezing biological sample technology is introduced into terahertz time-domain spectroscopy detection technique by topic, is proposed a kind of for detecting life
The terahertz time-domain spectroscopy detection device of object nerve samples can solve to encounter when prior art detection biological neural sample tired
Difficulty, can fundamentally evade the interference effect brought by the hydrone in biological neural, and detection device design it is tight,
It is reliable and stable, easy to operate.
The present invention provides a kind of terahertz time-domain spectroscopy detection device for detecting biological neural sample, described devices
Including：Nerve samples low temperature environment manufacturing cell, THz wave generate unit, THz wave detection unit and data analysis list
Member, wherein
Nerve samples low temperature environment manufacturing cell for placing nerve samples, and provides detection nerve samples terahertz
Hereby low temperature environment required when spectrum makes the freedom of motion of hydrogen bond freeze, to advise by the way that nerve samples are cooled to 77K
Keep away interference effect of the hydrogen bond to spectrum of hydrone formation in sample；
The THz wave generates unit, the broadband THz wave for generating detection nerve samples information；
The THz wave detection unit, for detecting the broadband Terahertz for carrying relevant information after nerve samples
Wave, and THz wave is converted into electric signal and obtains time-domain spectroscopy；The THz wave generating unit and THz wave detection are single
The position of member setting should meet：The broadband THz wave that THz wave generating unit generates can be incident on after nerve samples by too
Hertz wave detection unit detects；
The data analysis unit, the time-domain spectroscopy for being obtained to the THz wave detection unit carry out Fourier and turn
Get frequency domain spectra, the data analysis unit and THz wave detection unit Electricity Federation in return.
Further, nerve samples low temperature environment manufacturing cell include compression pump, Dewar container for liquefied nitrogen bottle, corrugated stainless steel tubing,
Nerve samples pond mistress and nerve samples pond interior room, wherein
The air entry of the compression pump opens in air, the exhaust outlet of the compression pump and the Dewar container for liquefied nitrogen bottle into
Gas port is connected；
The liquid outlet of the Dewar container for liquefied nitrogen bottle is connected with the Single port of the corrugated stainless steel tubing, the stainless steel ripple
The another port of pipe is connected with chamber inlet in chamber inlet outside the nerve samples pond and the nerve samples pond simultaneously；
Be provided in the middle part of the nerve samples pond mistress for THz wave by central through hole；In the nerve samples pond
Room is threaded through outside the nerve samples pond indoor and vertical with central through hole；Nerve samples pond mistress's opening releases are in sky
In gas, the nerve samples pond interior room opening releases are in air.
Further, the THz wave generation unit includes：Femto-second laser, laser beam splitter mirror, THz wave transmitting
Device, off axis paraboloidal mirror and pump light, wherein
To the laser beam splitter mirror, laser is divided into the laser light incident of femto-second laser outgoing by the laser beam splitter
Pump light and detection light；
The pump light of the laser beam splitter mirror outgoing is incident on the THz wave transmitter, inspires broadband terahertz
Hereby wave；
Reflection of the broadband THz wave through the off axis paraboloidal mirror that the THz wave transmitter is launched, it is incident
To being placed on the nerve samples of nerve samples pond interior room；
Further, the THz wave detection unit includes：Detect light, stepping delay motor, terahertz wave detector,
First laser speculum, second laser speculum, third laser mirror, the 4th laser mirror and the 5th laser mirror,
Wherein,
The THz wave generates the detection light that laser beam splitter mirror is emitted in unit and is reflected through the first laser speculum
Afterwards, it is incident on the second laser speculum for being fixed on the stepping delay motor successively and is passed on third laser mirror
It broadcasts, the movement by changing stepping delay motor changes light path delay；
Reflection of the laser of the stepping delay motor outgoing successively through the 4th laser mirror and the 5th laser mirror
After be incident on terahertz wave detector, through THz wave generate unit outgoing broadband THz wave after nerve samples quilt
Terahertz wave detector receives；
Further, the data analysis unit includes：Signal transmssion line and computer, the terahertz wave detector with
Computer is connected by signal transmssion line.
Further, the nerve samples pond mistress includes two cavitys, is provided with for THz wave in the middle part of the cavity
By central through hole, chamber inlet outside nerve samples pond, nerve samples pond mistress outlet are provided on the cavity；It is described two
Nerve samples pond interior room frame is provided between cavity.
Further, nerve samples pond mistress's material is copper.
Further, in hollow structure, (a nerve samples pond interior room part is provided with circle to nerve samples pond interior room
Trepanning), nerve samples pond interior room is provided with chamber inlet and the outlet of nerve samples pond interior room, the nerve in nerve samples pond
The round hole that sample cell interior room opens up is nerve samples frame, and silicon chip partition, the silicon chip are provided in the nerve samples frame
Partition covers polyethylene window, chamber inlet enters in nerve samples pond for fixing nerve samples on the nerve samples
Liquid nitrogen and nerve samples are conducted heat by silicon chip partition contact, freeze nerve samples, polyethylene window is for fixed neural sample
Product.
Further, the nerve samples pond interior room material is silicon.
A kind of terahertz time-domain spectroscopy detection device for detecting biological neural sample provided by the invention, by low temperature cold
Freeze biological sample technology and be introduced into terahertz time-domain spectroscopy detection technique, freezes hydrogen bond degree of freedom using low temperature (77K)
Characteristic forms low temperature environment around biological neural sample first, then utilize liquid nitrogen and biological neural sample by silicon chip every
Disconnecting is touched and is freezed, and the interference brought by the rotation and vibration of water molecules hydrogen bond in biological neural sample is farthest evaded
It influences, considerably reduces the spectrum detection limit of biomolecule in biological neural sample, and then improve and utilize terahertz time-domain light
Spectral technology detects the sensitivity of biological neural sample.The drying nitrogen being discharged after freezing biological neural sample is directly discharged to test
In air environment residing for device, device ambient air humidity is reduced, to eliminate moisture in air to the bad of spectrum
It influences, further improves system signal noise ratio.Meanwhile the device reasonable design, simple in structure, cost is relatively low, in use not
Need frequently to safeguard that there is good stability.The detection device is applicable to biological neural sample detection field, fills up
Terahertz time-domain spectroscopic technology is in the application blank in the field, the characteristics such as simple, easily operated with device.Meanwhile the device
It can be extended in the biology sample detection field of other water content biggers, there is wide extension foreground.Use the present invention
It is good etc. with highly sensitive, signal-to-noise ratio and stability when the device of offer carries out tera-hertz spectra detection to biological neural sample
Characteristic.
Description of the drawings
Fig. 1 is provided in an embodiment of the present invention for detecting the terahertz time-domain spectroscopy detection device of biological neural sample
Structural schematic diagram；
Fig. 2 is nerve samples pond mistress's structural schematic diagram provided in an embodiment of the present invention；
Fig. 3 is nerve samples pond mistress's cross-sectional view provided in an embodiment of the present invention；
Fig. 4 is nerve samples pond interior room structural schematic diagram provided in an embodiment of the present invention；
Fig. 5 is nerve samples pond interior room cross-sectional view provided in an embodiment of the present invention；
Fig. 6 is that tryptophan provided in an embodiment of the present invention acts on forming hydrogen bond structure schematic diagram with hydrone.
Reference numeral is in figure：
1, nerve samples low temperature environment manufacturing cell；2, THz wave generates unit；3, THz wave detection unit；4, number
According to analytic unit；11, compression pump；12, Dewar container for liquefied nitrogen bottle, 13, corrugated stainless steel tubing；14, nerve samples pond mistress；15, neural
Sample cell interior room；21, femto-second laser；22, laser beam splitter mirror；23, THz wave transmitter；24, off axis paraboloidal mirror；25,
Pump light；31, light is detected；32, stepping delay motor；33, terahertz wave detector；34, first laser speculum；35, second
Laser mirror；36, third laser mirror；37, the 4th laser mirror；38, the 5th laser mirror；41, computer；
42, signal transmssion line；141, chamber inlet outside nerve samples pond；142, nerve samples pond interior room frame；143, nerve samples pond mistress
Outlet；144, bolt；145, central through hole；146, cavity；151, chamber inlet in nerve samples pond；152, nerve samples pond interior room
Outlet；153, nerve samples frame；154, silicon chip separates；155, polyethylene window.
Specific implementation mode
For make present invention solves the technical problem that, the technical solution that uses and the technique effect that reaches it is clearer, below
The present invention is described in further detail in conjunction with the accompanying drawings and embodiments.It is understood that specific implementation described herein
Example is used only for explaining the present invention rather than limitation of the invention.It also should be noted that for ease of description, attached drawing
In only the parts related to the present invention are shown rather than full content.
Embodiment one
Fig. 1 is provided in an embodiment of the present invention for detecting the terahertz time-domain spectroscopy detection device of biological neural sample
Structural schematic diagram, more clear intuitive in order to explain the content of present invention, we are by biological neural sample cell details in attached drawing
In show, specifically, Fig. 2 be nerve samples pond mistress's structural schematic diagram provided in an embodiment of the present invention, Fig. 3 be the present invention
Nerve samples pond mistress's cross-sectional view that embodiment provides, Fig. 4 are in nerve samples pond provided in an embodiment of the present invention
Cell structure schematic diagram, Fig. 5 are nerve samples pond interior room cross-sectional view provided in an embodiment of the present invention, and Fig. 6 is the present invention
The tryptophan that embodiment provides acts on forming hydrogen bond structure schematic diagram with hydrone；
As shown in Figure 1, the terahertz time-domain spectroscopy detection device provided in an embodiment of the present invention for biological neural sample
Including：Nerve samples low temperature environment manufacturing cell 1, THz wave generate unit 2, THz wave detection unit 3 and data analysis
Unit 4, wherein
Nerve samples low temperature environment manufacturing cell 1 for placing nerve samples, and provides detection nerve samples terahertz
Hereby low temperature environment required when spectrum makes the freedom of motion of hydrogen bond freeze, to advise by the way that nerve samples are cooled to 77K
Keep away interference effect of the hydrogen bond to spectrum of hydrone formation in sample；The THz wave generates unit 2, for generating detection god
Broadband THz wave through sample message；The THz wave detection unit 3 carries phase for detecting after nerve samples
The broadband THz wave of information is closed, and THz wave is converted into electric signal and obtains time-domain spectroscopy, the THz wave occurs
The position that unit 2 and THz wave detection unit 3 are arranged should meet：The broadband Terahertz that THz wave generating unit 2 generates
Wave energy is detected after being incident on nerve samples by THz wave detection unit, specifically, the THz wave generating unit 2 and terahertz
Hereby wave detection unit 3 is located at the both sides of sample cell；The data analysis unit 4, for being obtained to the THz wave detection unit 3
To time-domain spectroscopy carry out Fourier transform and obtain frequency domain spectra, the data analysis unit 4 and 3 electricity of THz wave detection unit
Connection.
Wherein, nerve samples low temperature environment manufacturing cell 1 includes compression pump 11, Dewar container for liquefied nitrogen bottle 12, corrugated stainless steel tubing
13, nerve samples pond mistress 14 and nerve samples pond interior room 15, wherein the air entry of the compression pump 11 opens in air,
The exhaust outlet of the compression pump 11 is connected with the air inlet of the Dewar container for liquefied nitrogen bottle 12；The liquid outlet of the Dewar container for liquefied nitrogen bottle 12
Be connected with the Single port of the corrugated stainless steel tubing 13, the another port of the corrugated stainless steel tubing 13 simultaneously with the nerve
The outer chamber inlet 141 of sample cell is connected with chamber inlet 151 in the nerve samples pond；The nerve samples pond mistress 14 is in hollow
Structure, the nerve samples pond mistress outlet 143 open in air.Nerve samples pond interior room outlet 152 is opened in sky
In gas；
The material of the nerve samples pond mistress 14 is copper, and the nerve samples pond mistress 14 includes solid by bolt 144
Two fixed cavitys 146, the middle part of the cavity 146 be provided with for THz wave by central through hole 145, set on the cavity
It is equipped with chamber inlet 141 and nerve samples pond mistress outlet 143 outside the nerve samples pond being connected to inside cavity；Described two cavitys
Between be provided with nerve samples pond interior room frame 142.
The material of nerve samples pond interior room 15 is silicon, and nerve samples pond interior room 15 is arranged in nerve samples pond
On room frame 142, nerve samples pond interior room 15 is in hollow structure, and nerve samples pond interior room 15 is provided with and hollow structure
Chamber inlet 151 and nerve samples pond interior room outlet 152, state and are provided in nerve samples pond interior room 15 in the nerve samples pond of connection
Nerve samples frame 153, the liquid nitrogen and nerve samples that chamber inlet 151 enters in nerve samples pond pass through 154 contact of silicon chip partition
It conducts heat, freezes nerve samples, polyethylene window 155 is for fixing nerve samples.
The THz wave generates unit 2：Femto-second laser 21, laser beam splitter mirror 22, THz wave transmitter 23,
Off axis paraboloidal mirror 24 and pump light 25, wherein the laser light incident that the femto-second laser 21 is emitted to the laser beam splitter mirror
22, laser is divided into pump light 25 and detection light 31 by the laser beam splitter；The pump light 25 that the laser beam splitter mirror 22 is emitted
It is incident on the THz wave transmitter 23, inspires broadband THz wave；The THz wave transmitter 23 is launched
Reflection of the broadband THz wave through the off axis paraboloidal mirror 24, be incident on the neural sample for being placed in nerve samples pond interior room 15
On product；
The THz wave detection unit 3 includes：Detect light 31, stepping delay motor 32, terahertz wave detector 33, the
One laser mirror 34, second laser speculum 35, third laser mirror 36, the 4th laser mirror 37 and the 5th laser are anti-
Penetrate mirror 38, wherein it is anti-through the first laser that the THz wave generates the detection light 31 that laser beam splitter mirror 22 is emitted in unit 2
After penetrating the reflection of mirror 34, it is incident on the second laser speculum 35 being fixed on the stepping delay motor 32 and third laser successively
It is propagated on speculum 36, the movement by changing stepping delay motor 32 changes light path delay；The stepping delay motor
The laser of 32 outgoing is incident on THz wave spy after the reflection of the 4th laser mirror 37 and the 5th laser mirror 38 successively
Device 33 is surveyed, the broadband THz wave being emitted through THz wave generation unit 2 is after nerve samples by terahertz wave detector
33 receive；
The data analysis unit 4 includes：Computer 41 and signal transmssion line 42, wherein the signal transmssion line 42
One end is connected with the terahertz wave detector 33, and the other end of the signal transmssion line 42 is connected with the computer 41
It is logical, carry out subsequent data processing.
In the above scheme, the parameter of each component is：11 adjustable pressure 1-20Kpa of compression pump；Corrugated stainless steel tubing 13
Internal diameter is 8mm, outer diameter 10mm；The capacity of Dewar container for liquefied nitrogen bottle 12 is 4L；17 size of nerve samples pond mistress is 160 × 160
The copper cuboid of × 80mm, wall thickness 2mm, a diameter of 60mm of central through hole, 142 length of nerve samples pond interior room frame are 160mm,
Width is 80mm, and unilateral thickness is 2mm, and entrance 141 and the internal diameter for exporting 143 are 6mm, and outer diameter is 8mm, and bolt 144 is adopted
With M6 specifications；Interior room 15 length in nerve samples pond is 160mm, width 80mm, thickness 4mm, and 181 internal diameter of entrance is 6mm, outside
Diameter is 8mm, and outlet 182 is the rectangular outlet of 60 × 1mm, nerve samples frame 183 a diameter of 60mm, depth 2mm, silicon chip partition
Thickness is 0.5mm；21 wavelength of femto-second laser is 800nm, pulsewidth 45fs, repetition rate 1KHz, energy 3.2mJ；The
One laser mirror 34, second laser speculum 35, third laser mirror 36, the 4th laser mirror 37 and the 5th laser are anti-
It is 2 inches to penetrate mirror diameter, is deielectric-coating speculum, and reflectivity is more than 99% at centre wavelength 800nm；Stepping delay electricity
32 single stepping of machine is 75nm, stroke range 155mm, maximum speed 500mm/s；THz wave transmitter 23 is based on femtosecond laser
The output of 0.1-10THz broadband THz waves may be implemented in the principle for inducing air plasma radiation THz wave；Terahertz
Wave detector 33, which is based on air Third-order nonlinearity, can realize 0.1-10THz broadband THz wave coherent detections.
Provided by the present invention for detect biological neural sample terahertz time-domain spectroscopy detection device at work, first
System light path part is protected with a ransparent organic glass cover, drying nitrogen is filled with into cloche, is waited in cloche
When relative humidity drops to 5% or less, compression pump 11 is opened, adjusts pressure so that the liquid nitrogen in Dewar container for liquefied nitrogen bottle 12 is respectively from god
Enter nerve samples pond mistress 14 and nerve samples through chamber inlet outside sample cell 141 and the entrance 151 of nerve samples pond interior room
In pond interior room 15, ensure that there is drying nitrogen discharge in the outlet 142 of nerve samples pond mistress outlet 143 and nerve samples pond interior room,
This state is kept, until when sample cell ambient humidity drops to 2% or less, by suitable biological neural sample to be measured
(radius is less than 60mm, and thickness is less than 2mm) is put on nerve samples frame 153, and covers polyethylene window 155.Femtosecond is opened to swash
The power supply of light device 21, stepping delay motor 32, THz wave transmitter 23 and terahertz wave detector 33 so that THz wave produces
Raw unit 3 and THz wave detection unit 4 are started to work, and the operation of control stepping delay motor 32 makes the THz wave of generation
Enter terahertz wave detector 33 after penetrating nerve samples, and THz wave is converted into electric signal and obtains time-domain spectroscopy, data
Enter the progress of computer 41 Fourier transform by signal transmssion line 42 and obtains frequency domain spectra.
Terahertz time-domain spectroscopy detection device provided in this embodiment for detecting biological neural sample, by cryogenic freezing
Biological sample technology is introduced into terahertz time-domain spectroscopy detection technique, freezes hydrogen bond freedom of motion using low temperature (77K)
Characteristic, form low temperature environment around biological neural, liquid nitrogen and biological neural sample then utilized to pass through silicon chip partition contact
It is freezed, has fundamentally evaded the bad shadow brought by the rotation and vibration of hydrogen bond in hydrone in biological neural sample
It rings, considerably reduces the spectrum detection limit of biomolecule in biological neural sample.What is be discharged after freezing biological neural sample is dry
In air environment residing for the experimental provision of dry nitrogen direct emission, to reduce device ambient air humidity, to exclude sky
Harmful effect of the hydrone to spectrum, further improves system signal noise ratio in gas.Meanwhile the device reasonable design, structure letter
Single, cost is relatively low, need not frequently safeguard in use, has good stability.The detection device is applicable to biology
Nerve samples detection field has filled up the blank that terahertz time-domain spectroscopic technology detects in the field, has the operation is stable, operation
The simple and autgmentability characteristics such as by force.
Finally it should be noted that：The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
Present invention has been described in detail with reference to the aforementioned embodiments, it will be understood by those of ordinary skill in the art that：It is to preceding
It states the technical solution recorded in each embodiment to modify, either which part or all technical features is equally replaced
It changes, the range for various embodiments of the present invention technical solution that it does not separate the essence of the corresponding technical solution.
Claims (9)
1. a kind of terahertz time-domain spectroscopy detection device for detecting biological neural sample, which is characterized in that including：Neural sample
Product low temperature environment manufacturing cell, THz wave generating unit, THz wave detection unit and data analysis unit, wherein
Nerve samples low temperature environment manufacturing cell for placing nerve samples, and provides detection nerve samples terahertz light
Low temperature environment needed for time spectrum, to make the freedom of motion of hydrogen bond freeze, evades sample by the way that nerve samples are cooled to 77K
Interference effect of the hydrogen bond that hydrone is formed in product to spectrum；
The THz wave generating unit, the broadband THz wave for generating detection nerve samples relevant information；
The THz wave detection unit, for detecting the broadband THz wave for carrying relevant information after nerve samples,
And THz wave is converted into electric signal and obtains time-domain spectroscopy；The THz wave generating unit and THz wave detection unit are set
The position set should meet：The broadband THz wave that THz wave generating unit generates can be incident on after nerve samples by Terahertz
Wave detection unit detects；
The data analysis unit, the time-domain spectroscopy for being obtained to the THz wave detection unit carry out Fourier transform and obtain
To frequency domain spectra, the data analysis unit and THz wave detection unit Electricity Federation.
2. the terahertz time-domain spectroscopy detection device according to claim 1 for detecting biological neural sample, feature
It is, nerve samples low temperature environment manufacturing cell (1) includes compression pump (11), Dewar container for liquefied nitrogen bottle (12), corrugated stainless steel tubing
(13), nerve samples pond mistress (14) and nerve samples pond interior room (15), wherein
The air entry of the compression pump (11) opens in air, exhaust outlet and the Dewar container for liquefied nitrogen bottle of the compression pump (11)
(12) air inlet is connected；
The liquid outlet of the Dewar container for liquefied nitrogen bottle (12) is connected with the Single port of the corrugated stainless steel tubing (13), the stainless steel
The another port of bellows (13) simultaneously with chamber inlet in chamber inlet (141) outside the nerve samples pond and the nerve samples pond
(151) it is connected；
Be provided in the middle part of the nerve samples pond mistress (14) for THz wave by central through hole (145)；The nerve sample
Product pond interior room (15) is threaded through in the nerve samples pond mistress (14), and vertical with central through hole (145)；The nerve samples
Pond mistress exports (143) and opens in air, and it is open in air that nerve samples pond interior room exports (152).
3. the terahertz time-domain spectroscopy detection device according to claim 1 for detecting biological neural sample, feature
It is, the THz wave generates unit (2) and includes：Femto-second laser (21), laser beam splitter mirror (22), THz wave transmitter
(23), off axis paraboloidal mirror (24) and pump light (25), wherein
The laser light incident of the femto-second laser (21) outgoing is to the laser beam splitter mirror (22), and the laser beam splitter is by laser
It is divided into pump light (25) and detection light (31)；
The pump light (25) of laser beam splitter mirror (22) outgoing is incident on the THz wave transmitter (23), inspires width
Frequency band THz wave；
Reflection of the broadband THz wave through the off axis paraboloidal mirror (24) that the THz wave transmitter (23) is launched,
It is incident on the nerve samples for being placed in nerve samples pond interior room (15).
4. the terahertz time-domain spectroscopy detection device according to claim 1 for detecting biological neural sample, feature
It is, the THz wave detection unit (3) includes：Detect light (31), stepping delay motor (32), terahertz wave detector
(33), first laser speculum (34), second laser speculum (35), third laser mirror (36), the 4th laser mirror
(37) and the 5th laser mirror (38), wherein
The THz wave generates the detection light (31) that laser beam splitter mirror (22) is emitted in unit (2) and is reflected through the first laser
After mirror (34) reflection, it is incident on the second laser speculum (35) for being fixed on the stepping delay motor (32) successively and third swashs
Light reflection mirror is propagated on (36), and the movement by changing stepping delay motor (32) changes light path delay；
The laser that the stepping delay motor (32) is emitted is successively through the 4th laser mirror (37) and the 5th laser mirror
(38) it is incident on terahertz wave detector (33) after reflection, the broadband Terahertz that unit (2) is emitted is generated through THz wave
Wave is received after nerve samples by terahertz wave detector (33).
5. the terahertz time-domain spectroscopy detection device according to claim 1 for detecting biological neural sample, feature
It is, the data analysis unit (4) includes：Computer (41) and signal transmssion line (42), wherein
The terahertz wave detector (33) is connected with computer (41) by signal transmssion line (42).
6. the terahertz time-domain spectroscopy detection device according to claim 1 for detecting biological neural sample, feature
It is, the nerve samples pond mistress (14) includes two cavitys, is provided with for THz wave in the middle part of the cavity
Heart through-hole (145), chamber inlet (141) outside nerve samples pond are provided on the cavity, and nerve samples pond mistress exports (143)；
Nerve samples pond interior room frame (142) is provided between described two cavitys.
7. the terahertz time-domain spectroscopy detection device according to claim 1 for detecting biological neural sample, feature
It is, nerve samples pond mistress (14) material is copper.
8. the terahertz time-domain spectroscopy detection device according to claim 1 for detecting biological neural sample, feature
It is, nerve samples pond interior room (15) is in hollow structure, and nerve samples pond interior room (15) is provided with nerve samples pond
Interior chamber inlet (151) and nerve samples pond interior room outlet (152), it is god to state the round hole that nerve samples pond interior room (15) opens up
Through specimen holder (153), silicon chip partition (154) is provided in the nerve samples frame, the silicon chip partition (154) is for fixed god
Through sample, polyethylene window (155) is covered on the nerve samples.
9. the terahertz time-domain spectroscopy detection device according to claim 1 for detecting biological neural sample, feature
It is, the nerve samples pond interior room (15) material is silicon.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112345485A (en) * | 2020-10-30 | 2021-02-09 | 东莞理工学院 | Temperature-controlled sample pool and method for detecting conformation transition temperature of biomolecule solution |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203244962U (en) * | 2013-05-07 | 2013-10-23 | 中国石油大学(北京) | Hydrate reactor suitable for direct measurement of terahertz spectrum |
| CN104181105A (en) * | 2013-05-23 | 2014-12-03 | 中国科学院大连化学物理研究所 | Sample tank used for observation of liquid oxygen fluorescence spectrum |
| CN204269534U (en) * | 2014-10-23 | 2015-04-15 | 中国石油大学(北京) | For the device of laying temperature controllable liquid sample in terahertz light spectrometry |
| CN104583759A (en) * | 2012-03-22 | 2015-04-29 | 银诺维姆科学组织 | New method of dynamic spectroscopy under physiological conditions |
| CN205103680U (en) * | 2015-11-03 | 2016-03-23 | 中国人民大学 | Permanent concentration liquid sample cell temperature regulating device of steam balanced type |
| CN105738315A (en) * | 2016-04-07 | 2016-07-06 | 绍兴文理学院 | Terahertz device for real-time monitoring of biomolecule composition and content and measuring method thereof |
-
2018
- 2018-06-04 CN CN201810561830.XA patent/CN108760674B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104583759A (en) * | 2012-03-22 | 2015-04-29 | 银诺维姆科学组织 | New method of dynamic spectroscopy under physiological conditions |
| CN203244962U (en) * | 2013-05-07 | 2013-10-23 | 中国石油大学(北京) | Hydrate reactor suitable for direct measurement of terahertz spectrum |
| CN104181105A (en) * | 2013-05-23 | 2014-12-03 | 中国科学院大连化学物理研究所 | Sample tank used for observation of liquid oxygen fluorescence spectrum |
| CN204269534U (en) * | 2014-10-23 | 2015-04-15 | 中国石油大学(北京) | For the device of laying temperature controllable liquid sample in terahertz light spectrometry |
| CN205103680U (en) * | 2015-11-03 | 2016-03-23 | 中国人民大学 | Permanent concentration liquid sample cell temperature regulating device of steam balanced type |
| CN105738315A (en) * | 2016-04-07 | 2016-07-06 | 绍兴文理学院 | Terahertz device for real-time monitoring of biomolecule composition and content and measuring method thereof |
Non-Patent Citations (1)
| Title |
|---|
| HWAYEONG CHEON 等: "Terahertz molecular resonance of cancer DNA", 《SCIENTIFIC REPORTS》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112345485A (en) * | 2020-10-30 | 2021-02-09 | 东莞理工学院 | Temperature-controlled sample pool and method for detecting conformation transition temperature of biomolecule solution |
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